[Histonet] AgNOR

[Teresa Dominguez]

From: "Teresa Dominguez" <mariatere <@t> infovia.com.ar>
To: "prasad sbr prasad" <pathoprasad <@t> rediffmail.com>
Sent: Friday, March 12, 2004 9:38 PM
Subject: Re: [Histonet] AgNOR


> Sorry If I'm late for this, but at least I tried to help someone.
> This is the AgNOR method I had in my notes.
> Solutions:
>                A) Ag. Nitrate Sol.
>                     AgNO3................50 Gr.
>                     Destilled Water...100 ml
>
>                 B) Jelly
>                     Dest. Water........100 ml.
>                     Jelly........................2 Gr
>
>                 C) Formic Acid
>                      Formic Acid ...........1 ml.
>                      Dest. Water.........100 ml.
> Method: 1- Desparaffinize and hydrate to destilled water
>               2-Wash in dest. water (2 changes)
>               3-Place in this solution for 30 minutes, room temperature:
>                                                 One part of 2% Jelly ,
>                                                 One part of 1% Formic
Acid,
>                                                  Two part of 50%Ag NO3
>               4- Wash in hot dest. water
>               5- Rinse in dest. water
>               6- Counterstain, if desire with 0,5% Light Green
>               7- Deshydrate in95% Alcohol, Absolute Alcohol,and clear in
> Xylene, two
>                   changes each.
>               8- Mount with Permount
>
> Results:
>               NORs, M's G1 period: Dark Brown or black
>               Cytoplasms: Green or light brown
>
> Good Luck!!!
>
>
> H.T Maria T Dominguez
> Anatomy Pathology Service
> Hospital Regional Río Grande,
> Río Grande, Tierra del Fuego, Argentina
> 54- 02964-422086/88 Ext. 143
> ----- Original Message -----
> From: "prasad sbr prasad" <pathoprasad <@t> rediffmail.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Saturday, March 06, 2004 12:26 AM
> Subject: [Histonet] AgNOR
>
>
> >
> >
> > Dear List,
> >
> >   I shall be thankful to you if you send me the detaails of AgNOR
staining
> and scoring methods.
> >
> > with regards,
> > Prasad.
> > Dr.CSBR.Prasad,MD.,
> > Al Hakeem Polyclinic,
> > PO.BOX: 34985,
> > Riyadh-11478,
> > Saudi Arabia.
> > ************************************************
> > ************************************************
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>