[Histonet] Quality of tissues: freeze - thaw - freeze
Daniel Eberhard
daniel.eberhard <@t> uni-bielefeld.de
Sat Mar 13 02:57:36 CST 2004
Dear Listers,
some month ago I prepared some mouse tissues for cryo-sectioning without
fixing the tissues before.
Now I have found out that usual fixing of sections is not efficient for
our purposes, and
whole mount fixation (e.g. with pFA) would have been much better.
Thus my question:
Have some of you ever thawed unfixed tissue blocks,
fixed the tissues and re-frozen them? I´m afraid the
integrity of the tissue might be spoiled and of course:
some of the tissues are valuable and unique.
Any hints are welcome
thanks
Daniel.
--
(-)-(-)
--------------------------- \"/ ---
Dr. Daniel Eberhard =V=
Developmental Biology
& Molecular Pathology
Graduate Programe on Pattern Formation
University of Bielefeld
D 33501 Bielefeld/Germany
FAX: xx49(0)521-106-5654 (-)-(-)
--------------------------- \"/ ---
=V=
More information about the Histonet
mailing list