[Histonet] Toluene substitute

[Chan Wai Kam]

Hi Histonetters,

Our lab uses toluene in the tissue processor and as we are unable to have an overhead hood for this equipment, I'm wondering if there is any available toluene substitute we could use for specimen clearing. Another problem with leaving the solutions (alcohol and solvent) in the processor(carousel type) is the rapid rate of evaporation from the buckets.  Would appreciate any advice.  

Thanks
Julee Chan
Musculoskeletal Tissue Lab
Orthopaedic Surgery
National University of Singapore