Fwd: [Histonet] re: cryostat sectioning
Atoska S. Gentry
gentras <@t> vetmed.auburn.edu
Tue Mar 9 15:10:54 CST 2004
Please pardon my ignorance, but what kind of frozen tissues are you
sectioning on the sliding microtome, what brand & type sliding microtome
are you using, and how are you keeping your samples frozen for sectioning
on it? Thanks, Atoska
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>Hi Kathleen,
>
>I feel your pain. I've been cutting cryostat sections for almost 30
>years, and sometimes it can be completely maddening for no discernible
>reason.
>
>It could possibly be static as one Histonetter suggested. A Japanese
>fellow in our lab showed us that spraying Cytocool on the knife and roll
>plate really works. It's the ONLY thing that consistently works.
>
>Are your tissue blocks cryoprotected in sucrose? Have you tried a
>freshly made solution of your cryoprotectant in case the original was
>not made proplerly?
>
>We are a research lab, and we always cut our fixed frozen sections for
>immuno on a sliding microtome. We find this method much easier than the
>cryostat for fixed frozen. Do you have a sliding microtome available and
>have you tried it?
>
>Good luck,
>Mollie Lange
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Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL 36849
Phone# (334)844-5579 Fax# (334)844-5850
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