[Histonet] Faster luxol fast blue

[Barbara Stancel]

We preheat 45 ml of LFB for 20 seconds at full power in our microwave, place 
the slides in the stain and place in a 68C oven for 30 minutes. The rest of 
the stain is as printed in most manuals. Always works. Pathologists happy.

Histologically yours,

Barbara

Eastern Laboratory, Pathology
Athens, Georgia  30604


>From: Gayle Callis <gcallis <@t> montana.edu>
>To: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>, Histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Faster luxol fast blue
>Date: Tue, 09 Mar 2004 10:49:06 -0700
>
>Go to Sakura Finetek Histologic website, and search for Diane Sterchi's
>Modified Kluver-Barrera for Melin and Nerve Cells, unfortunately, I do not
>have the date nor issue.  Sections at 10 um thick.
>
>She did it in 3 hours following a preheating of Luxol fast blue to 60C,
>stain for 3 hrs in this hot LFB,  rinse in 95% ETOH to remove excess stain,
>rinse in distilled water, begin differentiation in 0.05% lithium carbonate,
>continue this step in 70% ethanol, until gray and white mater are
>distinguished.  This step is FAST! rinse well in distilled water,
>counterstain.
>
>Good luck.
>
>At 10:08 AM 3/9/2004 -0600, you wrote:
> >I have a doctor who needs a LFB stain today and the procedure I use
>requires that slides be left in the Luxol fast blue overnight.  Is there a
>way to speed that step up?
> >
> >Have a great day!!!!
> >
> >********************************************************************
> >Jordan W. Brod, HTL (ASCP)
> >Diagnostic Supervisor - Pathology
> >Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
> >jbrod <@t> tvmdl.tamu.edu
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Get a FREE online computer virus scan from McAfee when you click here. 
http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963