[Histonet] croystat sectioning

[Alan Bright]

Dear Kathleen,

It seem like you have a static problem, do you have an anti-static brush
or some other way of eliminating the static.?

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Kathleen A Spencer [mailto:kspencer <@t> utmem.edu] 
Sent: 08 March 2004 15:47
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] croystat sectioning


I have a  problem with cryostat sectioning that is driving me crazy. I
hope someone out there can help because we have exhausted our brains
coming up with solutions that have not helped. I am sectioning perfused
rat brain on a cryostat, 20u thick, for IHC on floating sections. I have
been doing this successfully for years. Now the problem is that they are
curling up into tight little rolls in the PBS, which make them
impossible to stain. They even curl as soon as they come off the knife
edge, so I can't even put them on slides. 
We have: tried several different formulas for the fix, 3 different
people doing the perfusion, 3 different kinds of PBS, warm PBS, cold
PBS, all possible knife angles, different knives, different side of the
knife, made the block colder, or warmer, thicker sections, thinner
sections, cut fast, cut slow, NOTHING HELPS! When we cut fresh frozen
brain to put on slides we do not have this problem. So, naturally we
think it is the fix, or the method, or the person doing the perfusion.
So, I had the expert do a perfusion and it still happened. At this point
I just wanted to go out and get drunk and I am not a drinking person. 
Can anyone help? All advice is appreciated.

Kathleen Spencer
Lab Manager
UTHSC


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