[Histonet] FW: [IHCRG] Very important question!!!!!!!!!!!!!!! !

Fri Mar 5 11:05:31 CST 2004

We had a very enlightening call from the Erbitux drug rep last week, and
from her statement, the drug will not even be offered to any patient without
documentation that the DAKO kit was used.  I have had to supply that
information to our research people who enter patients on the protocols
Possibly it might be due to the "newness" of this kit, but probably, from
Patti's numbers, reimbursement will be a big issue.  We were also advised to
use charge code 88342-22 to increase our reimbursement.

Jacquie Poteete MT(ASCP)QIHC, Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK
japoteete <@t> saintfrancis.com

> -----Original Message-----
> From:	Sebree Linda A. [SMTP:la.sebree <@t> hosp.wisc.edu]
> Sent:	Friday, March 05, 2004 8:14 AM
> To:	Histonet (E-mail)
> Subject:	[Histonet] FW: [IHCRG] Very important
> question!!!!!!!!!!!!!!!!
> 
> I thought these communiqués should be shared with the Histonet list
> server.
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Clinical & Research Laboratory
> DM223-VA
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX:  (608)262-7174
> 
> 
> 
> -----Original Message-----
> From: lualhati harkins [mailto:leharkins <@t> yahoo.com]
> Sent: Thursday, March 04, 2004 6:23 PM
> To: Patsy Ruegg; Rcartun <@t> harthosp.org; deb.vaneyck <@t> phci.org;
> ihcrg <@t> yahoogroups.com
> Cc: brian.kueck <@t> phci.org; david.ferguson <@t> phci.org;
> katherine.bayliss <@t> phci.org
> Subject: RE: [IHCRG] Very important question!!!!!!!!!!!!!!!!
> 
> 
> HI Patsy,
>  In addition to EGFr issue, i use Egfr antibody from Zymed and our
> pathologist like it. I also tried polyclonal  from Bio Care and it is very
> good too. Currently, we do not have plans to evaluate and EGFR kit, we are
> confident with our developed protocol for the EGFr antibody we are
> currently using. We did pretty good with The CAP Survey samples using
> EGFR. We placed confidently in good percent of agreement with results from
> other labs.
> Thank you,
> Loui
> 
> Patsy Ruegg <pruegg <@t> msn.com> wrote:
> Yes the rab. monoclonal egfr I am using is a sample from Lab Vision.  Have
> 
> you used it?  Any pointers?
> Patsy
> 
> 
> 
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 216
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> HP-303-644-4538
> 
> hm email pruegg <@t> msn.com
> wk email pruegg <@t> colobio.com
> 
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> ----Original Message Follows----
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> To: <pruegg <@t> msn.com>,<deb.vaneyck <@t> phci.org>, <ihcrg <@t> yahoogroups.com>
> CC: 
> <brian.kueck <@t> phci.org>,<david.ferguson <@t> phci.org>,<katherine.bayliss <@t> phci.o
> rg>
> Subject: RE: [IHCRG] Very important question!!!!!!!!!!!!!!!!
> Date: Thu, 04 Mar 2004 14:55:46 -0500
> 
> Yes, and it's very expensive!  We evaluated their EGFr kit a while ago
> along with two other mAbs (BioGenex and Zymed).  We actually prefer the
> Zymed antibody.  Patsy, is the rabbit mAb that you are using from Lab
> Vision?
> 
> Rich Cartun
> 
> >>> "Patsy Ruegg" <pruegg <@t> msn.com> 03/04/04 02:11PM >>>
> Deb,
> This is a very important issue and I hope that we all get fed back on
> this.
> I am using the rabbit monoclonal egfr antibody on  some research
> samples,
> but not the kit.  I have very little data yet to report.  Is DAKO
> selling
> the EGFR kit?
> Patsy
> 
> 
> 
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 216
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> HP-303-644-4538
> 
> hm email pruegg <@t> msn.com
> wk email pruegg <@t> colobio.com
> 
> This email is confidential and intended solely for the use of the
> Person(s)
> ('the intended recipient') to whom it was addressed. Any views or
> opinions
> presented are solely those of the author. It may contain information
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> Accordingly
> any dissemination, distribution, copying, or other use of this message,
> or
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> 
> 
> 
> ----Original Message Follows----
> From: "Van Eyck, Deb" <deb.vaneyck <@t> phci.org>
> To: "'ihcrg'" <ihcrg <@t> yahoogroups.com>
> CC: "Kueck, Brian D." <brian.kueck <@t> phci.org>,   "Bayliss, Katherine"
> <katherine.bayliss <@t> phci.org>,   "Ferguson, David J."
> <david.ferguson <@t> phci.org>
> Subject: [IHCRG] Very important question!!!!!!!!!!!!!!!!
> Date: Thu, 4 Mar 2004 10:26:38 -0600
> 
> Hi and I would like feedback from clinical folks out there--------have
> you
> started running your EGFR kits on patients yet????  How is the
> staining????
> I ran a number of random cases and seem to be getting darker staining
> and
> more cytoplasmic reaction than suggested.  My negs are great and
> clean-kit
> exactly followed.  My cervix is much darker than in picture in old
> guide and
> the diffuse cytoplasmic staining predominated most cases making the
> membrane
> staining hard to evaluate.  What is your experience?? Most of my cases
> are 6
> months or less tissue cases.
> 
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> ****************************************************************
> Please note: Protocol and technical information provided by the
> undersigned are developed on site with conditions limited to fixation of
> tissues,reagents,control tissues and execution of protocol which is
> characteristic only of our laboratory and therefore we do not bear neither
> responsibility nor liability for results outside of our laboratory. It is
> our courteous gesture to share
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> technologists.
> ******************************************************************
> Loui Harkins , M.S. QIHC
> Biologist, Immunohistochemistry/Path.& lab. Medicine
> DEpt. Of Veterans Affairs,700 South, 19th Street
> Birmingham, Alabama 35233
> (Research Consultant) Dept .of Surgery, Neurosurgery Division
> University of Alabama in Birmingham
> 205-933-8101 ext.6719; fax 205-558-4817 or 558-7034
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