[Histonet] FW: flow citometry protocol

[Serrano del Pozo, Erika]

> Hi histotechs,
> 
> I'm working with injured mice muscle, so it´s full of inflammatory cells
> at 2-5 days after injury. I´d like to know if anyone of you knows how to
> separate these inflammatory cells from a whole muscle to perform a flow
> citometry assay or where can I find a protocol to do it. Since now I've
> been doing IHC in frozen tissue because I only needed to check there were
> macrophages and T-lymphocytes, but now I need to count them and I don't
> know how to obtain "clean" cells to perform the experiment, I couldn´t
> find anything on the internet.
> 
> Thanks in advance,
> 
> 
> 	           Erika Serrano
> 
>   Centre de Regulació Genòmica
> Differenciation and Cancer Programme
> 	              Barcelona 
> 
>