[Histonet] Re: Histonet Digest, Vol 4, Issue 1
a-lisowski <@t> northwestern.edu
a-lisowski <@t> northwestern.edu
Mon Mar 1 13:40:58 CST 2004
==============Original message text===============
On Mon, 01 Mar 2004 12:00:04 pm CST
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Today's Topics:
1. (no subject) (Paula Wilder)
2. Acetylcholinesterase -Kit? (Gudrun Lang)
3. Hi everyone! (Teresa Dominguez)
4. Prostate Needle Biopsies (RSRICHMOND <@t> aol.com)
5. RE: Hi everyone! (Weems, Joyce)
6. Inga Hansson and ED1 antibody (Sharon Cooperman)
7. Re: Prostate Needle Biopsies (NICK KIRK)
8. apoptosis (Inga Hansson)
9. histological detection of protein carbonyl groups (Shaumik Adhya)
10. rat probe for insitu on mouse tissue? (yangpw <@t> umich.edu)
11. MeCP2 antibody (Mollie Lange)
12. Going to Toronto!!! (George Cole)
13. RE: Going to Toronto!!! (Morken, Tim - Labvision)
14. RE: Going to Toronto!!! (Barry R Rittman)
----------------------------------------------------------------------
Message: 1
Date: Sun, 29 Feb 2004 18:21:12 +0000
From: "Paula Wilder" <histo20 <@t> hotmail.com>
Subject: [Histonet] (no subject)
To: ccdub <@t> earthlink.net, histonet <@t> pathology.swmed.edu
Message-ID: <BAY8-F48G0fkbxCsyQ30000ba53 <@t> hotmail.com>
Content-Type: text/plain; format=flowed
Hi Cindy!
We also receive quite a few prostate biopsies A-M, but mostly A-F. Anyway,
the policy at our institution is no more than 3 biopsy pieces per cassette,
so if an A prostate biopsy contained 7 cores, then the PAs would make two
cassettes, one with 3 and other with 4 pieces. As a tech, I find this a
little difficult, since both A blocks would have to be embedded together to
ensure that 7 pieces were indeed found. The PAs also "ink" the tips of each
biopsy with a color dye. We use yellow, black, blue and green. We found
that the red ink color bled out to other biopsies. We do this because we
sometimes accession several prostate cases back to back, and although they
are grossed at different work stations, this is another way to ensure that
the accessioning is correct and that patients did not inadvertently get
reversed.
Paula Wilder
St. Joseph Medical Center
Towson, MD. 21204
_________________________________________________________________
Find and compare great deals on Broadband access at the MSN High-Speed
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------------------------------
Message: 2
Date: Sun, 29 Feb 2004 20:16:55 +0100
From: "Gudrun Lang" <gudrun.lang <@t> aon.at>
Subject: [Histonet] Acetylcholinesterase -Kit?
To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001601c3fef8$98178a90$eeeea8c0 <@t> SERVER>
Content-Type: text/plain; charset="iso-8859-1"
Can anyone recommend a Kit to perform Acetylcholinesterase on rectum biopsies?
Can anyone tell me a substitute for iso-OMPA with this reaction?
thanks in advance
Gudrun Lang, Austria
------------------------------
Message: 3
Date: Sun, 29 Feb 2004 23:24:13 -0300
From: Teresa Dominguez <mariatere <@t> infovia.com.ar>
Subject: [Histonet] Hi everyone!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <000a01c3ff34$4bafcaa0$277f46c8 <@t> FAMILIA>
Content-Type: text/plain; charset=iso-8859-1
Hi All,
I am a new suscriber in this list, I work in a Pathology Lab
at the Hospital of the city where I live. I am glade to be here, and I
would like to share with all of you my experiences, and ask to you
whatever I need as an advice.
See you in the list messages, bye for now,
Maria
P.S: sorry if I write something wrong, I am still studying English...
H.T Maria T Dominguez
Anatomy Pathology Service
Hospital Regional Río Grande,
Río Grande, Tierra del Fuego, Argentina
54- 02964-422086/88 Ext. 143
------------------------------
Message: 4
Date: Sun, 29 Feb 2004 22:23:47 EST
From: RSRICHMOND <@t> aol.com
Subject: [Histonet] Prostate Needle Biopsies
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1db.1b50c90c.2d7406c3 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Becky Garrison in Jacksonville FL observes:
>>Just this week, our Urology Center, in reviewing its QA measures, wanted to
know if it's OK for them to ink the specimen by color to reduce specimen
identification mixups. My first reaction was no because of concerns that
unknown
inks might interfere with fixation and ultimately IHC and the possibility of
crush artefact introduced by "non-pathology" hands.<<
I agree - no. I want them to handle the specimen as little as possible - the
ink needs to be added after fixation. They could use colored pens to mark the
outsides of the containers with a set of laboratory-prescribed colors that the
lab would use to select cassette and slide frosting colors.
I know that there are services that pre-ink the specimens, but I've never
actually seen it done.
Bob Richmond
Samurai Pathologist
Gastonia NC
------------------------------
Message: 5
Date: Sun, 29 Feb 2004 22:39:37 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Hi everyone!
To: 'Teresa Dominguez ' <mariatere <@t> infovia.com.ar>,
"'histonet-bounces <@t> lists.utsouthwestern.edu '"
<histonet-bounces <@t> lists.utsouthwestern.edu>,
"'histonet <@t> lists.utsouthwestern.edu '"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD63E1 <@t> exch2.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
Welcome, Maria!
Don't worry about your English. We're still studying too! And we still get
it wrong. Just hop in when you have a question.
Joyce
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Sent: 2/29/2004 9:24 PM
Subject: [Histonet] Hi everyone!
Hi All,
I am a new suscriber in this list, I work in a Pathology Lab
at the Hospital of the city where I live. I am glade to be here, and I
would like to share with all of you my experiences, and ask to you
whatever I need as an advice.
See you in the list messages, bye for now,
Maria
P.S: sorry if I write something wrong, I am still studying English...
H.T Maria T Dominguez
Anatomy Pathology Service
Hospital Regional Río Grande,
Río Grande, Tierra del Fuego, Argentina
54- 02964-422086/88 Ext.
143_______________________________________________
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------------------------------
Message: 6
Date: Sun, 29 Feb 2004 22:41:34 -0500
From: Sharon Cooperman <scoop <@t> mail.nih.gov>
Subject: [Histonet] Inga Hansson and ED1 antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <p06020408bc685dfd0b03@[156.40.160.118]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Hi. I'm writing to say thank you to Inga Hansson who told me about
using the ED1 antibody from Serotec for microglia on FFPE tissue with
citrate antigen retrieval. It also works great on bone marrow
macrophages and knowing about it saved me a huge amount of time. I
can't find Inga's email address to email her directly, so if you're
out there Inga, thanks.
--
Sharon Cooperman <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
------------------------------
Message: 7
Date: Mon, 1 Mar 2004 06:03:00 +0000 (GMT)
From: NICK KIRK <nick.kirk3 <@t> btopenworld.com>
Subject: Re: [Histonet] Prostate Needle Biopsies
To: RSRICHMOND <@t> aol.com, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040301060300.81766.qmail <@t> web86311.mail.ukl.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I agree with the sentiments below, it's difficult enough searching for a
translucent biopsy in a clear fluid, searching for a blue biopsy in a
blue fluid (or whatever colour the ink is) would be even more difficult.
We address the issue of prostate biopsies by giving the Urology
department a bag of biopsy sponges. When they take the needle cores they
then place them on a single damp biopsy sponge, when all the biopsies
have been taken, they then place the sponge into a formalin pot.
99% of the biopsies stay on the sponges and we only get the occasional
"stray", which is picked up easily, as we routinely check the pot and the
inside of the lid for any missed biopsies.
(It's surprising what will sometimes cling to a biopsy pot lid)
As for the other issues, well we routinely receive two biopsy pots, one
sampled from the left and the second from the right in annotated pots.
Each request card details the number of cores in each pot. Then at the
macroscopic description phase, the cores are counted and any
discrepancies noted and detailed in the report itself.
All the cores from each pot are placed in a single cassette and processed
as per normal. The resultant blocks are then serially cut with as many
sections as possible on each slide. We stain slides 1, 3, 5, 7 and 9 with
H&E, sections 2 and 4 are unstained and kept and filed, sections 6 and 8
are placed onto coated slides and the majority of these are then
immunostained for high molecular weight cytokeratin.
However, we recently did an audit on this process due to the increasing
numbers and time consuming nature of the work, to see if this was an
effective method.
We stained the slides as per normal and gave slides 1, 3 and 5 to the
Pathologist to read and diagnose. They then looked at slides 7 and 9 to
see if these slides made any difference to their diagnosis. The idea
being that if slides 7 and 9 made no difference to their diagnosis we
would change our working practice accordingley.
The results showed overwhelmingly that slides 7 and 9 made no difference
to the diagnosis in over 98% of cases and in the remaining 2% they didn't
change the diagnosis from benign to malignant, just a different degree of
benign disease.
Due to this data, we are currently changing our working practice so that
we don't have to cut so many sections, this will be more cost and time
effective and should have little or no effect on the end diagnosis.
After all, if we don't cut so many sections, there will still be the
oppertunity to cut further sections should the pathologist be uncertain
of the diagnosis.
Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
RSRICHMOND <@t> aol.com wrote:
Becky Garrison in Jacksonville FL observes:
>>Just this week, our Urology Center, in reviewing its QA measures, wanted to
know if it's OK for them to ink the specimen by color to reduce specimen
identification mixups. My first reaction was no because of concerns that
unknown
inks might interfere with fixation and ultimately IHC and the possibility of
crush artefact introduced by "non-pathology" hands.<<
I agree - no. I want them to handle the specimen as little as possible - the
ink needs to be added after fixation. They could use colored pens to mark the
outsides of the containers with a set of laboratory-prescribed colors that the
lab would use to select cassette and slide frosting colors.
I know that there are services that pre-ink the specimens, but I've never
actually seen it done.
Bob Richmond
Samurai Pathologist
Gastonia NC
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 1 Mar 2004 10:38:38 +0100
From: Inga Hansson <Inga.Hansson <@t> neuro.uu.se>
Subject: [Histonet] apoptosis
To: histonet <@t> pathology.swmed.edu
Message-ID: <a05111b05bc68b4775bd9@[130.238.32.246]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Hi everyone!
Is anyone using Apoptag kit on mouse cryos and have a problem with weak signal?
Any suggestions would be appreciated!
Thanks
Inga
--
Inga Hansson
Dept. neuroscience,
div. neurobiology
PO Box 587, BMC
Husargatan 3
S-751 23 Uppsala
SWEDEN
Phone: +46(18) 471 4384
Fax: +46(18)559017
------------------------------
Message: 9
Date: Mon, 1 Mar 2004 13:29:09 +0000
From: Shaumik Adhya <shaumik.adhya <@t> ucl.ac.uk>
Subject: [Histonet] histological detection of protein carbonyl groups
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1078147749.40433aa5a1b07 <@t> www.webmail.ucl.ac.uk>
Content-Type: text/plain; charset=ISO-8859-1
Hi Histonetters,
Does anyone know of a stain for detecting carbonyl groups on proteins as a
result of protein oxidation? All the methods I can find deal with carbonyl
content of proteins in minced samples, not tissue sections. I'd be grateful
for any ideas.
Shaumik
------------------------------
Message: 10
Date: Mon, 1 Mar 2004 10:31:03 -0500
From: yangpw <@t> umich.edu
Subject: [Histonet] rat probe for insitu on mouse tissue?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1078155063.40435737a8c60 <@t> mail.umich.edu>
Content-Type: text/plain; charset=ISO-8859-1
Good morning, everyone,
I have been doing double insitu on rat brain tissue (c-fos RNA rat probe
radioactive and enk RNA rat probe non-radioactive). And the staining works
fine. Recently, we are begining to try in situ on mouse brain tissue. Can I
just use rat RNA probes on mouse tissue? I have blasted the probes sequence,
they match rat c-fos/enk sequence very well, and match mouse c-fos/enk about
90%. Is this kind of match good enough to get good signal on mouse tissue? Does
anyone have any suggestion? Do I need to reduce the strigency of wash to retain
more signal in this case? If it is not good to use rat probe, can anyone
recommend any good mouse probe for c-fos and enkephalin? Thanks very much!
Pengwei Yang
BioPsychology Program
University of Michigan
Hi,
generally blast with a score of 90% would be a "go".
As far as stringency washes, extend time or/and temperature by few
degress so loosly bound probe will fell off. I have used probes designed
for human with primate tissue, rat probes with mouse tissue and have v.
good results.
Hope this helps
Andrew Lisowski
Director Molecular Technologies
Northwestern University
Chicago
a-lisowski <@t> northwestern.edu
------------------------------
Message: 11
Date: Mon, 01 Mar 2004 10:51:07 -0500
From: "Mollie Lange" <lange <@t> kennedykrieger.org>
Subject: [Histonet] MeCP2 antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s04315b4.087 <@t> gwia.kki.org>
Content-Type: text/plain; charset=US-ASCII
I am looking for a non-rabbit antibody to MeCP2 (methyl cpg binding
protein 2) for immunohistochemistry on frozen sections. Does anybody
know of one?
Thanks,
ML
------------------------------
Message: 12
Date: Mon, 1 Mar 2004 08:29:48 -0800
From: "George Cole" <georgecole <@t> ev1.net>
Subject: [Histonet] Going to Toronto!!!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c3ffaa$6ae08c90$034dbad0 <@t> hppav>
Content-Type: text/plain; charset="us-ascii"
Histonetters,
T O R O N T O !!!!
I have received an invitation to present a report on the new
methods of muscle and nerve biopsies that have been sent all over the
place----109 sent so far----at the National Histotechnology Convention
in Toronto next September. I will look forward to meeting those of you
who can come to that big get-together. I spoke at the 1978 convention in
San Francisco on silver techniques. That was a speech-only presentation
before the whole meeting. This time, there will he a lab set up for the
muscle and nerve confab. I'm already putting a video together that will
project on a large screen, and I mean to make most of it live so we can
get down to practical biopsy performance. It will be a good chance to
get acquainted, and to work out practical methods to improve the yield
of information from muscle and nerve biopsy tissues. It will be a three
hour lab. I'm already practicing my presentation on my wife. I've gone
through it 32 times already for her. My, It'll be nice to present to
folks that don't keep turning the TV up loud while I'm talking. I have
been reading every message on the Histonet since last July, when I
joined. And I have taken a cue from the messages you fellow techs send
to each other. I have reduced my total 3 hour presentation down to the
initial letters of every word----you know, like all of you immuno
processing techs ---it should be just an elegant up-to-the-minute
presentation. But truly folks, I will look forward to doing the best I
can to get some of these methods into public domain for the good of the
patients .I've been having the time of my life communicating with you
folks all over the world. Toronto will be the peek capper to my
histoteching. I already sleep hovering 14 inches over the
bed.---tension?--- naaaahhh---but If I can just bring it off in
Toronto, maybe I can look forward to coming in for a landing.------ See
you there, I hope!
georgecole <@t> ev1.net
------------------------------
Message: 13
Date: Mon, 1 Mar 2004 08:43:29 -0800
From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
Subject: RE: [Histonet] Going to Toronto!!!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<0556BE8AC5551E4E8AF6BB9E42509BA2177358 <@t> usca0082k08.labvision.apogent.com>
Content-Type: text/plain
George wrote:<<I'm already practicing my presentation on my wife. I've gone
through it 32 times already for her.>>
And only 29 weeks left 'till NSH.....
Tim Morken
-----Original Message-----
From: George Cole [mailto:georgecole <@t> ev1.net]
Sent: Monday, March 01, 2004 8:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Going to Toronto!!!
Histonetters,
T O R O N T O !!!!
I have received an invitation to present a report on the new
methods of muscle and nerve biopsies that have been sent all over the
place----109 sent so far----at the National Histotechnology Convention in
Toronto next September. I will look forward to meeting those of you who
can come to that big get-together. I spoke at the 1978 convention in San
Francisco on silver techniques. That was a speech-only presentation before
the whole meeting. This time, there will he a lab set up for the muscle and
nerve confab. I'm already putting a video together that will project on a
large screen, and I mean to make most of it live so we can get down to
practical biopsy performance. It will be a good chance to get acquainted,
and to work out practical methods to improve the yield of information from
muscle and nerve biopsy tissues. It will be a three hour lab. I'm already
practicing my presentation on my wife. I've gone through it 32 times already
for her. My, It'll be nice to present to folks that don't keep turning the
TV up loud while I'm talking. I have been reading every message on the
Histonet since last July, when I joined. And I have taken a cue from the
messages you fellow techs send to each other. I have reduced my total 3
hour presentation down to the initial letters of every word----you know,
like all of you immuno processing techs ---it should be just an elegant
up-to-the-minute presentation. But truly folks, I will look forward to doing
the best I can to get some of these methods into public domain for the good
of the patients .I've been having the time of my life communicating with you
folks all over the world. Toronto will be the peek capper to my
histoteching. I already sleep hovering 14 inches over the
bed.---tension?--- naaaahhh---but If I can just bring it off in Toronto,
maybe I can look forward to coming in for a landing.------ See you there, I
hope!
georgecole <@t> ev1.net
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 1 Mar 2004 10:51:36 -0600
From: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] Going to Toronto!!!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<566FB0B522443D43AF02D2ADBE35A6F063598B <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
George
Here in Houston we have rules that apply if you are going to have your
wife as a critic of a scientific presentation that you are planning to
give:
1. She should have a cattle prod to indicate times when you are
talking too much.
2. The presentation should only be rehearsed a maximum of 3 times.
3. You owe her big!!
(Now I think of it these are rules that my wife instituted).
Good luck on your presentation.
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of George
Cole
Sent: Monday, March 01, 2004 10:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Going to Toronto!!!
Histonetters,
T O R O N T O !!!!
I have received an invitation to present a report on the new
methods of muscle and nerve biopsies that have been sent all over the
place----109 sent so far----at the National Histotechnology Convention
in Toronto next September. I will look forward to meeting those of you
who can come to that big get-together. I spoke at the 1978 convention in
San Francisco on silver techniques. That was a speech-only presentation
before the whole meeting. This time, there will he a lab set up for the
muscle and nerve confab. I'm already putting a video together that will
project on a large screen, and I mean to make most of it live so we can
get down to practical biopsy performance. It will be a good chance to
get acquainted, and to work out practical methods to improve the yield
of information from muscle and nerve biopsy tissues. It will be a three
hour lab. I'm already practicing my presentation on my wife. I've gone
through it 32 times already for her. My, It'll be nice to present to
folks that don't keep turning the TV up loud while I'm talking. I have
been reading every message on the Histonet since last July, when I
joined. And I have taken a cue from the messages you fellow techs send
to each other. I have reduced my total 3 hour presentation down to the
initial letters of every word----you know, like all of you immuno
processing techs ---it should be just an elegant up-to-the-minute
presentation. But truly folks, I will look forward to doing the best I
can to get some of these methods into public domain for the good of the
patients .I've been having the time of my life communicating with you
folks all over the world. Toronto will be the peek capper to my
histoteching. I already sleep hovering 14 inches over the
bed.---tension?--- naaaahhh---but If I can just bring it off in
Toronto, maybe I can look forward to coming in for a landing.------ See
you there, I hope!
georgecole <@t> ev1.net
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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