[Histonet] Pollak's trichrome

[Bryan Hewlett]

Hi Bruce,

You are not doing anything wrong!
Using the reagents as listed, collagen would be green not blue as the
results state.
This must be a typo, either the dye listed, Light green SF, should be
Aniline blue and the results would be correct, or the results should be
collagen green. your choice.
Drop an e-mail to Bryan Llewellyn the stains file owner and indicate this,
I'm sure he will clarify.

Regards,

Bryan

----- Original Message -----
From: "Bruce W Brodersen" <bbroders <@t> unlnotes.unl.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 24, 2004 2:42 PM
Subject: [Histonet] Pollak's trichrome


>
>
>
>
> We've tried using the procedure listed on:
>
http://members.pgonline.com/~bryand/StainsFile/stain/conektv/tri_pollak.htm
>
> We keep having collagen stain green rather than blue, as the procedure
> describes.  Any suggestions as to what we are doing wrong?
> Thanks.
>
> Bruce W. Brodersen, DVM, PhD
> University of Nebraska Veterinary Diagnostic Center
> Fair St. & E. Campus Loop
> Lincoln, NE  68583-0907
>
> voice (402) 472-1434
> FAX (402 472-3094
>
>
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