[Histonet] Sudan Black B for lipofuscin?

[McCollough, Carol]

Greetings:

I have been asked to do a Sudan Black B targeting lipofuscin.  I know that lipofuscin can stain using oil red O, but am not familiar with using Sudan Black B for this purpose.  Is it an appropriate stain?  Is there a difference in protocol?

Thanks.

Regards -
Carol
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Carol B. McCollough, HT/HTL(ASCP)
Diagnostics & Histology Laboratory Manager
Oyster Disease Research Project
Fisheries Service
Maryland Department of Natural Resources
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, Maryland 21654
410-226-5193