I section fish on a regular basis and use the JB4 kit from Polysciences. I have experienced good results at 1-2 microns and stained with a PAS stain using metanil yellow as a counter stain. Feel free to email me any questions. Helen F. Wimer Wimer.Helen <@t> NMNH.SI.EDU Smithsonian Institution MSC NW Washington , DC 20560 (301) 238-3786