[Histonet] Flk-1 immunostaining protocol

[Wael Swelam]

Dear all,
I have been trying immunostaining for Flk-1 using different protocoles (Autoclave treatment using citrate buffer OR Tris-EDTA), enzyme treatment (proteinase K 5ug/ml); for Flk-1 monoclonal antibody (Santa cruse) i used 1:50 conc using Envesion detecting system. but i don't have the strong specific signal.
Can any one help
Thanks
Wael Swlam 
Ph.D student 
Niigata University,Japan