[Histonet] Von Kossa staining

Thu Jul 1 09:16:42 CDT 2004

Hello Fellow Histonetters!

 I have a quick question. I need to do a Von Kossa stain on some artery
vessels with calcium deposits present. I have embedded them in GMA not
MMA. I have a modified procedure for  bone to detect for calcium
deposits but  you remove the plastic. If anyone has a protocol they
would like to share for GMA it would be appreciated. Also, if anyone
knows of any other stains for GMA for calcium deposit detection it would
be greatly appreciated. Oh, I almost forgot the polymer I am using is
Technovit 7200.

Thank you,
 Tiffany