From Myri37 <@t> aol.com Thu Jul 1 03:07:02 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] Mc Neal's tetrachrome Message-ID: <07E27285.24B1AA8B.0005167B@aol.com> Hi everyone i have a recipe to make Mc neals tetrachrome (found on histonet) 0.5 g methylene blue 0.8 g azure A 0.1 g methyl?ne violet 250 ml methanol and 250 of glycerin, could someone tell me if it's 250 ml or 250 ?l or something else? i need to do it very soon, could you please answer me quickly. Thank you very much in advance. Myriam baali Natural implant From barbara.bublava <@t> meduniwien.ac.at Thu Jul 1 06:35:43 2004 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] silver stain Message-ID: <004e01c45f5f$8b35d7b0$1201a8c0@GERICHTS9XOZZ8> Hello everybody Is it true, that speciemens thought for silverstains have to be washed in distilled water between fixation and processing? Are there any special things to be done before silverstains? Regards Barbara, Vienna From jerry.santiago <@t> jax.ufl.edu Thu Jul 1 07:43:33 2004 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] We will miss him Message-ID: <921F55496EAE57458EF8B115C1E9DE6E0446D955@mail.jax.ufl.edu> It has been a sad time for the histology community with the loss of Steve Slap. He did so much for our field and no matter when you needed him, he was there to help out. We were one of the fortunate societies that had the opportunity to have Steve at our meetings. We asked and he delivered. He will always be remembered as a pioneer of the new generation of Histotechnology. Sincerely, Florida Society for Histotechnology From Myri37 <@t> aol.com Thu Jul 1 07:47:23 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] MacNeals tetrachrome Message-ID: <69E2BE8D.19F7AD2A.0005167B@aol.com> Hi everyone i have a recipe to make Mc neals tetrachrome (found on histonet) 0.5 g methylene blue 0.8 g azure A 0.1 g methyl?ne violet 250 ml methanol and 250 of glycerin, could someone tell me if it's 250 ml or 250 ?l or something else? i need to do it very soon, could you please answer me quickly. Thank you very much in advance. Myriam baali Natural implant From JQB7 <@t> CDC.GOV Thu Jul 1 08:02:27 2004 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] silver stain Message-ID: We have never washed specimens between fixation and processing and we do a lot of silver stains. We actually receive many of our specimens already in block form. What type of silver stains are you asking about regarding "special things" to be done? Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Bublava Sent: Thursday, July 01, 2004 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain Hello everybody Is it true, that speciemens thought for silverstains have to be washed in distilled water between fixation and processing? Are there any special things to be done before silverstains? Regards Barbara, Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Thu Jul 1 08:02:27 2004 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] silver stain Message-ID: We have never washed specimens between fixation and processing and we do a lot of silver stains. We actually receive many of our specimens already in block form. What type of silver stains are you asking about regarding "special things" to be done? Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Bublava Sent: Thursday, July 01, 2004 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain Hello everybody Is it true, that speciemens thought for silverstains have to be washed in distilled water between fixation and processing? Are there any special things to be done before silverstains? Regards Barbara, Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 08:52:43 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] weird brownish stuff in AEC slides Message-ID: <2056946547.1088689963971.JavaMail.osg@spnode33> I have yet another question, guys. I've been asking a lot of questions about blocking endogenous peroxidase,but now I have a new situation. I'm still using the ABC peroxidase method staining sheep placenta (formalin fixed, paraffin embedded) for eNOS and using AEC as a chromogen and gill's hematoxylin (blued with tap water and water with 2 or 3 drops of ammonia) as a counterstain. (btw, still blocking peroxidase with 30% peroxide in methanol, since my professors are away and haven't been able to order anything better) I have the lovely red and blue contrast in most parts of my slides, but I also am getting areas of brownish-black staining (kinda DAB colored, although I'm not using any DAB). I'm not exactly sure where the brown comes from this and whether its a problem with my AEC, my hematoxylin or something else. Anyone have any ideas? Thanks in advance, y'all have already been really helpful. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From dwalker <@t> selway.umt.edu Thu Jul 1 08:53:59 2004 From: dwalker <@t> selway.umt.edu (David Walker) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] Thank you Herb Message-ID: A big thank you to Herb and anyone else that helped to fix the overload, Huzzah!!! Dave -- David Walker, MS Microbiology Staff Scientist CEHS/CSFN School of Pharmacy and Allied Health Sciences University of Montana Missoula Montana (406) 243-2225 Fax (406) 243-2807 From mucram11 <@t> earthlink.net Thu Jul 1 09:12:17 2004 From: mucram11 <@t> earthlink.net (Pamela Marcum) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] An explanation of The Histonet Mail Loop Message-ID: <410-22004741141217765@earthlink.net> Herb and Linda, Thank you for getting us all back online and our e-mail in boxes clear again. The service provided by HistoNet is appreciated very much. I am sure we often drive you up the proverbial wall with our failures to unsubscribe and leave these out of office replies. You are very patient and supply us with a very good forum for discussion. Education and a place to ask questions is so important to all of us we sometimes take it for granted until it has a problem. Thanks Again, Pam Marcum > [Original Message] > From: Herb Hagler > To: > Date: 6/30/2004 6:34:49 PM > Subject: [Histonet] An explanation of The Histonet Mail Loop > > I wanted to thank those of you who have hung in there during the trials > the last 24 hrs. > > I wanted to let you know what happened: > > A person that everyone is familiar with now went on vacation and left > an "out of office" notice on their email account. This can be a huge > problem as we experienced first hand. Instead of a reply to the sender > of the email, they setup a reply to the histonet list. The reply to > list was posted, that message was sent from histonet to the persons > vacation reply, which replied to the list, which generated another > response.....millions of emails later everything slowed to a crawl. > > Please, please if you go on vacation and must leave a reply to tell > everyone that you are out of the office, remember to unsubscribe from > any list servers before you leave and give it a 30 minute test before > you finally go out the door so you don't become known and loved by so > many people and every server admin on the planet. > > I think that you get the picture. This was a big time mail loop which > in a matter of a few hours filled up everyones email quotas, filled our > admin message boxes, filled up the server, and all of this happened at > the end of the day here in Dallas during heavy rains when everyone that > could fix it was trying to get home. > > So we think things are quiet now. We are getting fewer bounces, so > would some of you gentle people please begin asking your questions > again about histology, employment and other interesting issues. > > Questions about why is my mail box full of junk messages, should be > obvious to everyone that something went wrong in geek land, and we have > identified the problem(99% confidence) and hopefully things will return > to normal by tomorrow afternoon when I switch the server from manual to > auto. > > Thanks and cheers, > Herb > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JQB7 <@t> CDC.GOV Thu Jul 1 08:02:27 2004 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] silver stain Message-ID: We have never washed specimens between fixation and processing and we do a lot of silver stains. We actually receive many of our specimens already in block form. What type of silver stains are you asking about regarding "special things" to be done? Jeanine Bartlett, HT(ASCP) Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Bublava Sent: Thursday, July 01, 2004 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain Hello everybody Is it true, that speciemens thought for silverstains have to be washed in distilled water between fixation and processing? Are there any special things to be done before silverstains? Regards Barbara, Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu Jul 1 09:16:11 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] weird brownish stuff in AEC slides References: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: <003b01c45f75$f5defae0$78065486@vdl220FAC> Is it brownish, as in a khaki color? If so, that usually is a phenomenon with AEC called metachromasia... a change in the precipitate color due to molecules of chromogen being in too close in proximity. Try diluting out your primary antibody, so the Ag/Ab complexes aren't so close together. Jan Shivers ----- Original Message ----- From: "SMITH,REBEKAH FELICIA" To: Sent: Thursday, July 01, 2004 8:52 AM Subject: [Histonet] weird brownish stuff in AEC slides > I have yet another question, guys. I've been asking a lot of > questions about blocking endogenous peroxidase,but now I have a > new situation. I'm still using the ABC peroxidase method staining > sheep placenta (formalin fixed, paraffin embedded) for eNOS and > using AEC as a chromogen and gill's hematoxylin (blued with tap > water and water with 2 or 3 drops of ammonia) as a counterstain. > (btw, still blocking peroxidase with 30% peroxide in methanol, > since my professors are away and haven't been able to order > anything better) I have the lovely red and blue contrast in most > parts of my slides, but I also am getting areas of brownish-black > staining (kinda DAB colored, although I'm not using any DAB). I'm > not exactly sure where the brown comes from this and whether its a > problem with my AEC, my hematoxylin or something else. Anyone have > any ideas? Thanks in advance, y'all have already been really > helpful. > > -- > SMITH,REBEKAH FELICIA > "You are a child of the universe, no less than the trees and the > stars > You have a right to be here and whether or not it is clear to you, > no doubt the universe is unfolding as it should. Therefore be at > peace with G-d, whatever you conceive Him to be. And whatever your > labors and aspirations,in the noisy confusion of life, keep peace > in your soul.-Max Ehrmann,"Desiderata" > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Tiffany.L.Sheffield <@t> uth.tmc.edu Thu Jul 1 09:16:42 2004 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Tiffany L Sheffield) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] Von Kossa staining Message-ID: <40E41CC9.2D98F96F@uth.tmc.edu> Hello Fellow Histonetters! I have a quick question. I need to do a Von Kossa stain on some artery vessels with calcium deposits present. I have embedded them in GMA not MMA. I have a modified procedure for bone to detect for calcium deposits but you remove the plastic. If anyone has a protocol they would like to share for GMA it would be appreciated. Also, if anyone knows of any other stains for GMA for calcium deposit detection it would be greatly appreciated. Oh, I almost forgot the polymer I am using is Technovit 7200. Thank you, Tiffany From bhewlett <@t> cogeco.ca Thu Jul 1 10:11:30 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] weird brownish stuff in AEC slides References: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: <004201c45f7d$b1573650$6500a8c0@mainbox> Rebekah, I believe that what you are describing is classic so-called metachromasia of the AEC reaction product. Metachromasia of the AEC reaction product is seen as a progressive change in colour from the normal rose-red to red-brown, followed by brown, yellow-brown, then brownish-green to yellowish-green. Metachromasia occurs in areas of high enzyme density (concentration). Enzyme density is influenced by both the local antigen density and the amount of attached primary antibody that is the target for the detection reagents. Therefore, the concentration of the primary antibody directly implies the local enzyme concentration, on the basis of a given antigenic density in the section. At low concentrations of peroxidase enzyme, it has been proposed1 that the reaction ends in the formation of a stable polymeric complex of red colour (Wursters Red), since the decay to a precipitate occurs more rapidly than further enzymatic oxidation. High concentrations of peroxidase accelerate the oxido-reductive process to the extent that there is insufficient time for the stable red polymer to form and precipitate. Instead, further rapid oxidation results in the formation and precipitation of a yellowish-green quinone di-imine product. Intermediate colours are a result of both reactions. Metachromasia in high antigen density areas is more prone to occur at temperatures above 24?C. Slight cooling of the AEC working solution will often correct this. If metachromasia in high antigen density areas persists despite lowering the temperature, or if some antibodies consistently demonstrate the phenomenon, reduction of the detection threshold by further dilution of the primary antibody is necessary. We often see this metachromatic effect during the optimization process for new antibodies. Re-titration of the primary antibody to a lower concentration always restores the rose-red colour. Bryan Reference. 1 Koretz K, Leman J, Brandt I, M?ller P: Metachromasia of 3-amino-9-ethylcarbazole (AEC) and its prevention in immunoperoxidase techniques. Histochemistry 1987; 86: 471-478. ----- Original Message ----- From: "SMITH,REBEKAH FELICIA" To: Sent: Thursday, July 01, 2004 9:52 AM Subject: [Histonet] weird brownish stuff in AEC slides > I have yet another question, guys. I've been asking a lot of > questions about blocking endogenous peroxidase,but now I have a > new situation. I'm still using the ABC peroxidase method staining > sheep placenta (formalin fixed, paraffin embedded) for eNOS and > using AEC as a chromogen and gill's hematoxylin (blued with tap > water and water with 2 or 3 drops of ammonia) as a counterstain. > (btw, still blocking peroxidase with 30% peroxide in methanol, > since my professors are away and haven't been able to order > anything better) I have the lovely red and blue contrast in most > parts of my slides, but I also am getting areas of brownish-black > staining (kinda DAB colored, although I'm not using any DAB). I'm > not exactly sure where the brown comes from this and whether its a > problem with my AEC, my hematoxylin or something else. Anyone have > any ideas? Thanks in advance, y'all have already been really > helpful. > > -- > SMITH,REBEKAH FELICIA > "You are a child of the universe, no less than the trees and the > stars > You have a right to be here and whether or not it is clear to you, > no doubt the universe is unfolding as it should. Therefore be at > peace with G-d, whatever you conceive Him to be. And whatever your > labors and aspirations,in the noisy confusion of life, keep peace > in your soul.-Max Ehrmann,"Desiderata" > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Jul 1 10:16:40 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:39 2005 Subject: [Histonet] weird brownish stuff in AEC slides In-Reply-To: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: Rebekah, How old is your AEC and when do you make it up? I used to get tan-brown precipitate when I made up the AEC too far in advance. Also, have you changed lot numbers or vendors recently? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of SMITH,REBEKAH FELICIA Sent: Thursday, July 01, 2004 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weird brownish stuff in AEC slides I have yet another question, guys. I've been asking a lot of questions about blocking endogenous peroxidase,but now I have a new situation. I'm still using the ABC peroxidase method staining sheep placenta (formalin fixed, paraffin embedded) for eNOS and using AEC as a chromogen and gill's hematoxylin (blued with tap water and water with 2 or 3 drops of ammonia) as a counterstain. (btw, still blocking peroxidase with 30% peroxide in methanol, since my professors are away and haven't been able to order anything better) I have the lovely red and blue contrast in most parts of my slides, but I also am getting areas of brownish-black staining (kinda DAB colored, although I'm not using any DAB). I'm not exactly sure where the brown comes from this and whether its a problem with my AEC, my hematoxylin or something else. Anyone have any ideas? Thanks in advance, y'all have already been really helpful. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmontara <@t> temple.edu Thu Jul 1 10:17:27 2004 From: tmontara <@t> temple.edu (T. Montara) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Re: Histonet Digest, Vol 7, Issue 92 References: <200406301322.BQV17601@po-smtp2.temple.edu> Message-ID: <003e01c45f7e$8756b0a0$ae2bf79b@thunderbolt> ----- Original Message ----- From: To: Sent: Wednesday, June 30, 2004 9:22 AM Subject: Histonet Digest, Vol 7, Issue 92 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. 2 questions (Jennifer Sipes) > 2. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 3. Guinea pig mast cells: (tony.j.savage@gsk.com) > 4. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 5. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 6. Job Opportunities in Histology Latest Update 06/30/04 > (Pam Barker (extension 234)) > 7. Florida License (Terry Murphy) > 8. Cleaning Metal Cassettes (Billie Zimmerman) > 9. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 10. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 11. unscubscribe (Marnie Whiteside) > 12. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 13. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 14. Re: New immunostainer (CrochiereSteve@aol.com) > 15. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > 16. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 30 Jun 2004 05:02:20 -0700 (PDT) > From: Jennifer Sipes > Subject: [Histonet] 2 questions > To: histonet@lists.utsouthwestern.edu > Message-ID: <20040630120220.2710.qmail@web60609.mail.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > My name is Jennifer. I have recently taken on the task of doing histology for my lab. I have just 2 questions: > > 1. Does anyone have a protocol for Toludine Blue staining? > > 2. A girl in my lab is cryo fixing monkey lung. The procedure goes as follows: > Each lung is sectioned into 9 parts. Of the total 18 lung sections, only 5% of > them go for cryosectioning. They are interested in the the large airways. > She is having a problem with getting decent cuts. Currently, she is submerging the > small pieces into OCT overnight at 4 C then mounting them. Does anyone happen > to have a protocol better than that. I told her that that was the incorrest way, but she > said that was the protocol she was given. Please remember that the rest of the > tissue is going for Real Time PCR, RNA extraction, etc. > > I would greatly appreciate any information that you folks could give me. Thnak you for your time. > > > > > Jennifer K. Sipes > Sr. Laboratory Technician > Johns Hopkins University > Ross Research Building Rm 929 > 720 Rutland Avenue > Baltimore, MD 21205 > phone: 410-614-0131 > cell: 443-413-0853 > fax: 410-955-9677 > e-mail: jengirl1014@yahoo.com > > Jennifer; Here is a Toluidine Blue protocol. The first is an aqueous mount, the second can be mounted in a resin. Solution'\; Toluidine blue 0.5% in 20% ethanol 1. Deparaffinize to water. 2. Toluidine blue 5 to 20 minutes. 3. Wash briefly in tap water 4.Coverslip from water. These slides are not permanent. 1.Follow first three steps as above 2.Then two changes each of 95%, Absolute, Xylene each one minute. Mount in Permount Hope this helps Thelma > > > > > > --------------------------------- > Do you Yahoo!? > New and Improved Yahoo! Mail - 100MB free storage! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Wed, 30 Jun 2004 03:11:54 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Wed, 30 Jun 2004 10:51:20 +0100 > From: tony.j.savage@gsk.com > Subject: [Histonet] Guinea pig mast cells: > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > My group is trying to find a good method for demonstrating guinea pig mast > cells which appear morphologically different to human and rodent mast > cells. I have been unable to find an antibody which demonstrates guinea > pig mast cells and the metachromatic tinctorial methods show only weak > staining. Does anyone have a good method to share with us for > demonstrating guinea pig mast cells, tinctorially or > immunohistochemically? > Kind Regards, > Tony Savage > Histopathology Group > Asthma Biology Department. > RIRP CEDD. > GlaxoSmithKline Medicines Research Centre, > Gunnelswood Road, > STEVENAGE, > Hertfordshire. > SG1 2NY > tel. +44 (0)1438 764117 > fax. +44 (0)1438 764782 > email. Tony.J.Savage@gsk.com > mobile +44 07753609835 > http://ukdiscovery.gsk.com/histopathology/default.htm > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Wed, 30 Jun 2004 06:43:54 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 30 Jun 2004 01:35:54 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 29 Jun 2004 21:01:18 -0400 > From: Pam Barker (extension 234) > Subject: [Histonet] Job Opportunities in Histology Latest Update > 06/30/04 > To: Histonetters > Message-ID: > Content-Type: Text/Plain > > Hi Histonetters, I hope you have a Happy 4th of July. > > Here is the latest update on opportunities with some of my best clients throughout the US who are seeking Histology Supervisors, Histo Technologists and Histo Technicians. These are positions as direct employees of our client. There are fulltime 40 hour per week positions.. As a direct employee of one of our clients you will be provided with full benefits including Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative sign-on bonus. I have part time and temporary positions as well. > > I have supervisory, team lead and bench positions. These positions require HTL or HT certification or registry eligibility. > > Here are some of my HOTTEST Histo Tech and Histology Supervisor positions: > 1. Tampa Bay, FL Area - MOHS Tech > 2. Ft. Lauderdale, FL - Histo Tech > 3. North Central Georgia - Histo Tech > 4. Central Pennsylvania - Histo Tech multiple openings > 5. Western Pennsylvania - Histo Tech > 6. Central Alabama - Histology Supervisor > 7. Southern Arizona - Histo Tech multiple openings and locations > 8. Southern Maine - Immunohistochemistry Tech > 9. Southern Maine - Histo Tech > 10. Northern California - Histo Tech (1 full time and 1 part time) > 11. Western Missouri - Histo Tech > 12. Eastern North Carolina - Histo Tech > 13. Central Virginia - Histo Tech (TEMP) > > If you are interested in these jobs, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well. I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. > > Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. > > However, if you are interested in any of the jobs above, please call me. > > Happy 4th of July!! > > Pam - 800 466 9919 ext 234 > > --------------------------------------------------------- > Ategra Systems Inc > Specialists in Permanent & Contract Staffing > > Learn More About Ategra: > > > Pam Barker > Senior Lab Recruiter > Ategra Systems Inc > Specialists in Permanent & Contract Staffing > 7085 University Blvd. > Winter Park, FL 32792 > > VOICE: 407-671-5800 ext 234 > TOLLFREE: 800-466-9919 ext 234 > EMAIL: pam@ategra.com > > To Learn More About Ategra: > http://www.ategra.com > -------------------------------------------------------------------------- ------------------------ > If you received this by mistake, or if you wish not to hear from me, > please shoot me a mail to let me know and I'll not mail you again. > -------------------------------------------------------------------------- ------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Tue, 29 Jun 2004 16:10:08 -0700 (PDT) > From: Terry Murphy > Subject: [Histonet] Florida License > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20040629231008.88913.qmail@web52304.mail.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hi. > > I am working as a traveling temporary Histotech. My temp agency has an opportunity in FL and I do not have a FL License. Does anyone know if there is a way to obtain a temporary FL License? > > Thanks > > Terry Murphy > > > --------------------------------- > Do you Yahoo!? > Yahoo! Mail - 50x more storage than other providers! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 29 Jun 2004 14:49:45 -0400 > From: "Billie Zimmerman" > Subject: [Histonet] Cleaning Metal Cassettes > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I'd like to know the procedure for cleaning metal cassettes. > Thanks, > Billie Zimmerman > Medical College of GA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 30 Jun 2004 00:22:53 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Wed, 30 Jun 2004 05:38:53 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Wed, 30 Jun 2004 07:00:47 -0400 > From: "Marnie Whiteside" > Subject: [Histonet] unscubscribe > To: > Message-ID: > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Wed, 30 Jun 2004 01:35:54 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 13 > Date: Wed, 30 Jun 2004 02:53:53 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Tue, 29 Jun 2004 21:41:06 EDT > From: CrochiereSteve@aol.com > Subject: Re: [Histonet] New immunostainer > To: histo@sdspathology.com.au, histonet@lists.utsouthwestern.edu > Message-ID: <147.2d469ff0.2e137432@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > I'm quite happy with my new Nemesis from Biocare Medical. > It has the highest slide and reagent capacity that I could find and the > reagents are much less expensive than the othe company's which I had been using. > > > > Steven M. Crochiere, HT(ASCP) > Histology Supervisor > LifePath Partners @ Mercy Medical Center > Springfield, MA 01104 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 15 > Date: Wed, 30 Jun 2004 01:05:54 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > 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Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Wed, 30 Jun 2004 02:36:54 -0500 > From: Kathy Abels > Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > I will be out of the office starting 06/30/2004 and will not return until > 07/12/2004. > > I will respond to your message when I return. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > 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_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 7, Issue 92 > *************************************** From nperson211 <@t> comcast.net Thu Jul 1 10:18:04 2004 From: nperson211 <@t> comcast.net (nperson211@comcast.net) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] out of office postings Message-ID: <070120041518.25240.40E42B2C000A3745000062982200734840CECECD02019C9D0A9F02@comcast.net> Histonetters, Considering our recent experience with the "out of office" run amok, why don't we agree that sending an "out of office" posting to the Histonet is really unnecessary? Anyone who will be away will have already informed those people who actually need to know. We, as a group DO NOT need this information. I believe that our intrepid list administrators would be happy to know that we will refrain from this practice in the future. Nancy Lemke Hermelin Brain Tumor Center Henry Ford Hospital Detroit From JNocito <@t> Pathreflab.com Thu Jul 1 10:07:18 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Cornea special stains In-Reply-To: <45.fa0f236.2e14cf92@aol.com> Message-ID: Steven, Once in a while, a doctor orders a PAS for Fungus, but we don't perform these routinely. Joe Nocito -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of CrochiereSteve@aol.com Sent: Wednesday, June 30, 2004 9:23 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Cornea special stains Hi, Are there any "routine" special stains done on cornea transplants which have failed and been removed? I've started to get more than "one in a blue-moon" submitted to the lab and was wondering if there are any other stains to do other than H&E to show the cause for rejection. Thanks, Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From haldana <@t> unimoron.edu.ar Thu Jul 1 10:22:41 2004 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Manual for the vibratome Lancer series 1000 Message-ID: <001c01c45f7f$40689ea0$a904a8c0@um.edu> Does anybody have the manual for the old vibratome Lancer Series 1000? Thanks in advance. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html From katri <@t> cogeco.ca Thu Jul 1 10:32:31 2004 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] weird brownish stuff in AEC slides References: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: <001801c45f80$a0313ad0$6a9a9618@Katri> Hi Rebekah, One possibility is formalin pigment, which appears as a brownish black extracellular pigment, but it would be present also in your negative controls. Katri Katri Tuomala St.Joseph's Health Centre Hamilton, Ontario, Canada ----- Original Message ----- From: "SMITH,REBEKAH FELICIA" To: Sent: Thursday, July 01, 2004 9:52 AM Subject: [Histonet] weird brownish stuff in AEC slides > I have yet another question, guys. I've been asking a lot of > questions about blocking endogenous peroxidase,but now I have a > new situation. I'm still using the ABC peroxidase method staining > sheep placenta (formalin fixed, paraffin embedded) for eNOS and > using AEC as a chromogen and gill's hematoxylin (blued with tap > water and water with 2 or 3 drops of ammonia) as a counterstain. > (btw, still blocking peroxidase with 30% peroxide in methanol, > since my professors are away and haven't been able to order > anything better) I have the lovely red and blue contrast in most > parts of my slides, but I also am getting areas of brownish-black > staining (kinda DAB colored, although I'm not using any DAB). I'm > not exactly sure where the brown comes from this and whether its a > problem with my AEC, my hematoxylin or something else. Anyone have > any ideas? Thanks in advance, y'all have already been really > helpful. > > -- > SMITH,REBEKAH FELICIA > "You are a child of the universe, no less than the trees and the > stars > You have a right to be here and whether or not it is clear to you, > no doubt the universe is unfolding as it should. Therefore be at > peace with G-d, whatever you conceive Him to be. And whatever your > labors and aspirations,in the noisy confusion of life, keep peace > in your soul.-Max Ehrmann,"Desiderata" > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJJ <@t> Stowers-Institute.org Thu Jul 1 10:33:10 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Re: weird brownish stuff in AEC slides Message-ID: <1123426498-41872443@pathology.swmed.edu> Rebekah, Do you have a picture of the artifact? Could it be formalin pigment in the bloody areas of the sample? Do you see it in the negative control? P.S. Lordy I hope this doesn't repeat. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From ryaskovich <@t> dir.nidcr.nih.gov Thu Jul 1 10:35:16 2004 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Von Kossa staining Message-ID: <8F3AB322628548428A992EFB0E80F5D3A2F09B@nihexchange8.nih.gov> Tiffany, I have done Von-Kossa's on MMA thick about 200 microns or more specimens whole not mounted on slides. Didn't remove any plastic just dropped the specimens right into the silver solution and into a window in the sun. Got wonderful positive results I would run a test slide on the GMA slide first and see what you get. Good luck! Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neuronal Gene Expression Section > ---------- > From: Tiffany L Sheffield > Sent: Thursday, July 1, 2004 9:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Von Kossa staining > > <> > Hello Fellow Histonetters! > > I have a quick question. I need to do a Von Kossa stain on some artery > vessels with calcium deposits present. I have embedded them in GMA not > MMA. I have a modified procedure for bone to detect for calcium > deposits but you remove the plastic. If anyone has a protocol they > would like to share for GMA it would be appreciated. Also, if anyone > knows of any other stains for GMA for calcium deposit detection it would > be greatly appreciated. Oh, I almost forgot the polymer I am using is > Technovit 7200. > > Thank you, > Tiffany > > From info <@t> instrumedics.com Thu Jul 1 10:42:54 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Re: Histonet Digest, Vol 8, Issue 1 References: Message-ID: <017e01c45f82$134e43e0$6401a8c0@INSTRUMEDICS22> Felicia, The brownish stained structures perhaps are lipofuscin granules which are common in many tissues, especially from older animals. Bernice schiller@instrumedics.com ----- Original Message ----- From: To: Sent: Thursday, July 01, 2004 10:36 AM Subject: Histonet Digest, Vol 8, Issue 1 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > ---------------------------------------------------------------------------- ---- > Today's Topics: > > 1. Collette holder for an old MT5000 Ultramicrotome (Vickroy, Jim) > 2. Davidson's (Dorothy.L.Webb@HealthPartners.Com) > 3. Monkey lung question (Jennifer Sipes) > 4. Collette holder for an old MT5000 Ultramicrotome (Vickroy, Jim) > 5. Monkey lung question (Jennifer Sipes) > 6. Davidson's (Dorothy.L.Webb@HealthPartners.Com) > 7. Collette holder for an old MT5000 Ultramicrotome (Vickroy, Jim) > 8. Monkey lung question (Jennifer Sipes) > 9. Davidson's (Dorothy.L.Webb@HealthPartners.Com) > 10. RE: [BULK] - [Histonet] Repetitions (Connie McManus) > 11. Parasympathetic and Sympathetic (Cameron Hogan) > 12. An explanation of The Histonet Mail Loop (Herb Hagler) > 13. An explanation of The Histonet Mail Loop (Herb Hagler) > 14. RE: [BULK] - [Histonet] Repetitions (Connie McManus) > 15. Parasympathetic and Sympathetic (Cameron Hogan) > 16. Parasympathetic and Sympathetic (Cameron Hogan) > 17. Histo position in Southeast Florida (pathrm35@adelphia.net) > 18. Re: kathy abels (marsha r price) > 19. Cornea special stains (CrochiereSteve@aol.com) > 20. silver stain (Barbara Bublava) > 21. We will miss him (Santiago, Jerry) > 22. MacNeals tetrachrome (Myri37@aol.com) > 23. Mc Neal's tetrachrome (Myri37@aol.com) > 24. RE: silver stain (Bartlett, Jeanine) > 25. RE: silver stain (Bartlett, Jeanine) > 26. Cornea special stains (CrochiereSteve@aol.com) > 27. weird brownish stuff in AEC slides (SMITH,REBEKAH FELICIA) > 28. Thank you Herb (David Walker) > 29. RE: An explanation of The Histonet Mail Loop (Pamela Marcum) > 30. RE: silver stain (Bartlett, Jeanine) > 31. Re: weird brownish stuff in AEC slides (Jan Shivers) > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Thu Jul 1 10:49:34 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] silver stain Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111358@mius2.medlan.cam.ac.uk> Use very clean glassware and good quality distilled water and reagents. I have never washed in DW between fixation and processing but do wash your sections in distilled water prior to staining. It can't hurt to wash the samples in DW after fixation either. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Barbara Bublava [mailto:barbara.bublava@meduniwien.ac.at] Sent: Thursday, July 01, 2004 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain Hello everybody Is it true, that speciemens thought for silverstains have to be washed in distilled water between fixation and processing? Are there any special things to be done before silverstains? Regards Barbara, Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JColCLEFA <@t> aol.com Thu Jul 1 11:09:13 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Corneas Message-ID: <1db.255922ff.2e159129@aol.com> We usually do a PAS/ lt green (fungus ctl) for all corneas. We found an amebic cyst once. (pond swimming after xplant=bad idea. From rockbeki <@t> ufl.edu Thu Jul 1 11:19:58 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] weird brownish stuff in AEC slides Message-ID: <1740210325.1088698798127.JavaMail.osg@spnode33> I will try cooling the AEC a bit. I probably am using a kinda high concentration of eNOS antibody (10 ug antibody/ per 1ml goat serum), but when I try lower concentrations (I've tried 5ug, 2ug,2.5 ug,.25 ug, and .025 ug), I get too little staining. There is definitely a lot of peroxidase already present in this tissue, so I've been blocking for 1 hour in 1:10 30% peroxide to methanol. On Thu Jul 01 11:11:30 EDT 2004, Bryan Hewlett wrote: > Rebekah, > > I believe that what you are describing is classic so-called > metachromasia of > the AEC reaction product. > Metachromasia of the AEC reaction product is seen as a > progressive change in > colour from the normal rose-red to red-brown, followed by brown, > yellow-brown, then brownish-green to yellowish-green. > > Metachromasia occurs in areas of high enzyme density > (concentration). Enzyme > density is influenced by both the local antigen density and the > amount of > attached primary antibody that is the target for the detection > reagents. > Therefore, the concentration of the primary antibody directly > implies the > local enzyme concentration, on the basis of a given antigenic > density in the > section. > > > > At low concentrations of peroxidase enzyme, it has been proposed1 > that the > reaction ends in the formation of a stable polymeric complex of > red colour > (Wursters Red), since the decay to a precipitate occurs more > rapidly than > further enzymatic oxidation. > > High concentrations of peroxidase accelerate the oxido-reductive > process to > the extent that there is insufficient time for the stable red > polymer to > form and precipitate. Instead, further rapid oxidation results in > the > formation and precipitation of a yellowish-green quinone di-imine > product. > Intermediate colours are a result of both reactions. > > > > Metachromasia in high antigen density areas is more prone to > occur at > temperatures above 24?C. > > Slight cooling of the AEC working solution will often correct > this. > > If metachromasia in high antigen density areas persists despite > lowering the > temperature, > > or if some antibodies consistently demonstrate the phenomenon, > reduction of > the detection > > threshold by further dilution of the primary antibody is > necessary. > > > > We often see this metachromatic effect during the optimization > process for > new antibodies. > > Re-titration of the primary antibody to a lower concentration > always > restores the rose-red colour. > > > > Bryan > > > > > > Reference. > > 1 Koretz K, Leman J, Brandt I, M?ller P: Metachromasia of > 3-amino-9-ethylcarbazole (AEC) and its prevention in > immunoperoxidase > techniques. Histochemistry 1987; 86: 471-478. > > > > > > > > > > ----- Original Message ----- > From: "SMITH,REBEKAH FELICIA" > To: > Sent: Thursday, July 01, 2004 9:52 AM > Subject: [Histonet] weird brownish stuff in AEC slides > > >> I have yet another question, guys. I've been asking a lot of >> questions about blocking endogenous peroxidase,but now I have a >> new situation. I'm still using the ABC peroxidase method staining >> sheep placenta (formalin fixed, paraffin embedded) for eNOS and >> using AEC as a chromogen and gill's hematoxylin (blued with tap >> water and water with 2 or 3 drops of ammonia) as a counterstain. >> (btw, still blocking peroxidase with 30% peroxide in methanol, >> since my professors are away and haven't been able to order >> anything better) I have the lovely red and blue contrast in most >> parts of my slides, but I also am getting areas of brownish-black >> staining (kinda DAB colored, although I'm not using any DAB). I'm >> not exactly sure where the brown comes from this and whether its >> a >> problem with my AEC, my hematoxylin or something else. Anyone >> have >> any ideas? Thanks in advance, y'all have already been really >> helpful. >> >> -- >> SMITH,REBEKAH FELICIA >> "You are a child of the universe, no less than the trees and the >> stars >> You have a right to be here and whether or not it is clear to >> you, >> no doubt the universe is unfolding as it should. Therefore be at >> peace with G-d, whatever you conceive Him to be. And whatever >> your >> labors and aspirations,in the noisy confusion of life, keep peace >> in your soul.-Max Ehrmann,"Desiderata" >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From kaburns <@t> med.unc.edu Thu Jul 1 11:34:51 2004 From: kaburns <@t> med.unc.edu (Kim Burns) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Monkey lung Message-ID: <000501c45f89$556d4800$0b2e1398@peds.med.unc.edu> You may want to consider devoting one lobe of the lung for frozen sections. You would tie off the lobe and then inlfate it with diuted OCT. Freeze the lobe over liquid nitrogen. The frozen lobe then can be sawed into slices and the areas of interest taken for frozen sectioning. We have obtained beautiful morphology of human lung ( large, small airway and parenchyma ) using this technique. Kimberlie Burns Cystic Fibrosis/Pulmonary Reseach Center The University of North Carolina at Chapel Hill From Pathologyarts <@t> aol.com Thu Jul 1 11:35:33 2004 From: Pathologyarts <@t> aol.com (Pathologyarts@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] looking for a specimen bottle Message-ID: <1d2.24f040a1.2e159755@aol.com> i'm looking for a prefilled bottle that is 3" tall and 1" wide. looked all over the world with no luck, anyone seen these anywhere? thanks for your help, curt From pruegg <@t> colobio.com Thu Jul 1 11:30:42 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Jan Minshew Message-ID: Jan if you are out there please contact me at pruegg@msn.com Thanks, Patsy From bhewlett <@t> cogeco.ca Thu Jul 1 10:11:30 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] weird brownish stuff in AEC slides References: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: <004201c45f7d$b1573650$6500a8c0@mainbox> Rebekah, I believe that what you are describing is classic so-called metachromasia of the AEC reaction product. Metachromasia of the AEC reaction product is seen as a progressive change in colour from the normal rose-red to red-brown, followed by brown, yellow-brown, then brownish-green to yellowish-green. Metachromasia occurs in areas of high enzyme density (concentration). Enzyme density is influenced by both the local antigen density and the amount of attached primary antibody that is the target for the detection reagents. Therefore, the concentration of the primary antibody directly implies the local enzyme concentration, on the basis of a given antigenic density in the section. At low concentrations of peroxidase enzyme, it has been proposed1 that the reaction ends in the formation of a stable polymeric complex of red colour (Wursters Red), since the decay to a precipitate occurs more rapidly than further enzymatic oxidation. High concentrations of peroxidase accelerate the oxido-reductive process to the extent that there is insufficient time for the stable red polymer to form and precipitate. Instead, further rapid oxidation results in the formation and precipitation of a yellowish-green quinone di-imine product. Intermediate colours are a result of both reactions. Metachromasia in high antigen density areas is more prone to occur at temperatures above 24?C. Slight cooling of the AEC working solution will often correct this. If metachromasia in high antigen density areas persists despite lowering the temperature, or if some antibodies consistently demonstrate the phenomenon, reduction of the detection threshold by further dilution of the primary antibody is necessary. We often see this metachromatic effect during the optimization process for new antibodies. Re-titration of the primary antibody to a lower concentration always restores the rose-red colour. Bryan Reference. 1 Koretz K, Leman J, Brandt I, M?ller P: Metachromasia of 3-amino-9-ethylcarbazole (AEC) and its prevention in immunoperoxidase techniques. Histochemistry 1987; 86: 471-478. ----- Original Message ----- From: "SMITH,REBEKAH FELICIA" To: Sent: Thursday, July 01, 2004 9:52 AM Subject: [Histonet] weird brownish stuff in AEC slides > I have yet another question, guys. I've been asking a lot of > questions about blocking endogenous peroxidase,but now I have a > new situation. I'm still using the ABC peroxidase method staining > sheep placenta (formalin fixed, paraffin embedded) for eNOS and > using AEC as a chromogen and gill's hematoxylin (blued with tap > water and water with 2 or 3 drops of ammonia) as a counterstain. > (btw, still blocking peroxidase with 30% peroxide in methanol, > since my professors are away and haven't been able to order > anything better) I have the lovely red and blue contrast in most > parts of my slides, but I also am getting areas of brownish-black > staining (kinda DAB colored, although I'm not using any DAB). I'm > not exactly sure where the brown comes from this and whether its a > problem with my AEC, my hematoxylin or something else. Anyone have > any ideas? Thanks in advance, y'all have already been really > helpful. > > -- > SMITH,REBEKAH FELICIA > "You are a child of the universe, no less than the trees and the > stars > You have a right to be here and whether or not it is clear to you, > no doubt the universe is unfolding as it should. Therefore be at > peace with G-d, whatever you conceive Him to be. And whatever your > labors and aspirations,in the noisy confusion of life, keep peace > in your soul.-Max Ehrmann,"Desiderata" > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Jul 1 10:16:40 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] weird brownish stuff in AEC slides In-Reply-To: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: Rebekah, How old is your AEC and when do you make it up? I used to get tan-brown precipitate when I made up the AEC too far in advance. Also, have you changed lot numbers or vendors recently? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of SMITH,REBEKAH FELICIA Sent: Thursday, July 01, 2004 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weird brownish stuff in AEC slides I have yet another question, guys. I've been asking a lot of questions about blocking endogenous peroxidase,but now I have a new situation. I'm still using the ABC peroxidase method staining sheep placenta (formalin fixed, paraffin embedded) for eNOS and using AEC as a chromogen and gill's hematoxylin (blued with tap water and water with 2 or 3 drops of ammonia) as a counterstain. (btw, still blocking peroxidase with 30% peroxide in methanol, since my professors are away and haven't been able to order anything better) I have the lovely red and blue contrast in most parts of my slides, but I also am getting areas of brownish-black staining (kinda DAB colored, although I'm not using any DAB). I'm not exactly sure where the brown comes from this and whether its a problem with my AEC, my hematoxylin or something else. Anyone have any ideas? Thanks in advance, y'all have already been really helpful. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JColCLEFA <@t> aol.com Thu Jul 1 11:09:13 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Corneas Message-ID: <1db.255922ff.2e159129@aol.com> We usually do a PAS/ lt green (fungus ctl) for all corneas. We found an amebic cyst once. (pond swimming after xplant=bad idea. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathologyarts <@t> aol.com Thu Jul 1 11:35:33 2004 From: Pathologyarts <@t> aol.com (Pathologyarts@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] looking for a specimen bottle Message-ID: <1d2.24f040a1.2e159755@aol.com> i'm looking for a prefilled bottle that is 3" tall and 1" wide. looked all over the world with no luck, anyone seen these anywhere? thanks for your help, curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 11:19:58 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] weird brownish stuff in AEC slides Message-ID: <1740210325.1088698798127.JavaMail.osg@spnode33> I will try cooling the AEC a bit. I probably am using a kinda high concentration of eNOS antibody (10 ug antibody/ per 1ml goat serum), but when I try lower concentrations (I've tried 5ug, 2ug,2.5 ug,.25 ug, and .025 ug), I get too little staining. There is definitely a lot of peroxidase already present in this tissue, so I've been blocking for 1 hour in 1:10 30% peroxide to methanol. On Thu Jul 01 11:11:30 EDT 2004, Bryan Hewlett wrote: > Rebekah, > > I believe that what you are describing is classic so-called > metachromasia of > the AEC reaction product. > Metachromasia of the AEC reaction product is seen as a > progressive change in > colour from the normal rose-red to red-brown, followed by brown, > yellow-brown, then brownish-green to yellowish-green. > > Metachromasia occurs in areas of high enzyme density > (concentration). Enzyme > density is influenced by both the local antigen density and the > amount of > attached primary antibody that is the target for the detection > reagents. > Therefore, the concentration of the primary antibody directly > implies the > local enzyme concentration, on the basis of a given antigenic > density in the > section. > > > > At low concentrations of peroxidase enzyme, it has been proposed1 > that the > reaction ends in the formation of a stable polymeric complex of > red colour > (Wursters Red), since the decay to a precipitate occurs more > rapidly than > further enzymatic oxidation. > > High concentrations of peroxidase accelerate the oxido-reductive > process to > the extent that there is insufficient time for the stable red > polymer to > form and precipitate. Instead, further rapid oxidation results in > the > formation and precipitation of a yellowish-green quinone di-imine > product. > Intermediate colours are a result of both reactions. > > > > Metachromasia in high antigen density areas is more prone to > occur at > temperatures above 24?C. > > Slight cooling of the AEC working solution will often correct > this. > > If metachromasia in high antigen density areas persists despite > lowering the > temperature, > > or if some antibodies consistently demonstrate the phenomenon, > reduction of > the detection > > threshold by further dilution of the primary antibody is > necessary. > > > > We often see this metachromatic effect during the optimization > process for > new antibodies. > > Re-titration of the primary antibody to a lower concentration > always > restores the rose-red colour. > > > > Bryan > > > > > > Reference. > > 1 Koretz K, Leman J, Brandt I, M?ller P: Metachromasia of > 3-amino-9-ethylcarbazole (AEC) and its prevention in > immunoperoxidase > techniques. Histochemistry 1987; 86: 471-478. > > > > > > > > > > ----- Original Message ----- > From: "SMITH,REBEKAH FELICIA" > To: > Sent: Thursday, July 01, 2004 9:52 AM > Subject: [Histonet] weird brownish stuff in AEC slides > > >> I have yet another question, guys. I've been asking a lot of >> questions about blocking endogenous peroxidase,but now I have a >> new situation. I'm still using the ABC peroxidase method staining >> sheep placenta (formalin fixed, paraffin embedded) for eNOS and >> using AEC as a chromogen and gill's hematoxylin (blued with tap >> water and water with 2 or 3 drops of ammonia) as a counterstain. >> (btw, still blocking peroxidase with 30% peroxide in methanol, >> since my professors are away and haven't been able to order >> anything better) I have the lovely red and blue contrast in most >> parts of my slides, but I also am getting areas of brownish-black >> staining (kinda DAB colored, although I'm not using any DAB). I'm >> not exactly sure where the brown comes from this and whether its >> a >> problem with my AEC, my hematoxylin or something else. Anyone >> have >> any ideas? Thanks in advance, y'all have already been really >> helpful. >> >> -- >> SMITH,REBEKAH FELICIA >> "You are a child of the universe, no less than the trees and the >> stars >> You have a right to be here and whether or not it is clear to >> you, >> no doubt the universe is unfolding as it should. Therefore be at >> peace with G-d, whatever you conceive Him to be. And whatever >> your >> labors and aspirations,in the noisy confusion of life, keep peace >> in your soul.-Max Ehrmann,"Desiderata" >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Thu Jul 1 11:48:05 2004 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] looking for a specimen bottle In-Reply-To: <1d2.24f040a1.2e159755@aol.com> Message-ID: <002e01c45f8b$2e7a8210$8861ba92@padlspsu.psu.edu> Try Evergreen. I can't find their catalog right now but I know they have a web site. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathologyarts@aol.com Sent: Thursday, July 01, 2004 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for a specimen bottle i'm looking for a prefilled bottle that is 3" tall and 1" wide. looked all over the world with no luck, anyone seen these anywhere? thanks for your help, curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Thu Jul 1 12:04:54 2004 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: <001101c45f8d$8806c8f0$6c01a8c0@new001> What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group From haldana <@t> unimoron.edu.ar Thu Jul 1 10:22:41 2004 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Manual for the vibratome Lancer series 1000 Message-ID: <001c01c45f7f$40689ea0$a904a8c0@um.edu> Does anybody have the manual for the old vibratome Lancer Series 1000? Thanks in advance. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Thu Jul 1 10:35:16 2004 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Von Kossa staining Message-ID: <8F3AB322628548428A992EFB0E80F5D3A2F09B@nihexchange8.nih.gov> Tiffany, I have done Von-Kossa's on MMA thick about 200 microns or more specimens whole not mounted on slides. Didn't remove any plastic just dropped the specimens right into the silver solution and into a window in the sun. Got wonderful positive results I would run a test slide on the GMA slide first and see what you get. Good luck! Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neuronal Gene Expression Section > ---------- > From: Tiffany L Sheffield > Sent: Thursday, July 1, 2004 9:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Von Kossa staining > > <> > Hello Fellow Histonetters! > > I have a quick question. I need to do a Von Kossa stain on some artery > vessels with calcium deposits present. I have embedded them in GMA not > MMA. I have a modified procedure for bone to detect for calcium > deposits but you remove the plastic. If anyone has a protocol they > would like to share for GMA it would be appreciated. Also, if anyone > knows of any other stains for GMA for calcium deposit detection it would > be greatly appreciated. Oh, I almost forgot the polymer I am using is > Technovit 7200. > > Thank you, > Tiffany > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Thu Jul 1 11:53:32 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] CD31 on porcine Message-ID: Does anyone know of a CD31 or CD34 procedure that will work on ffpe pig tissue? Patsy From JNocito <@t> Pathreflab.com Thu Jul 1 10:07:18 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Cornea special stains In-Reply-To: <45.fa0f236.2e14cf92@aol.com> Message-ID: Steven, Once in a while, a doctor orders a PAS for Fungus, but we don't perform these routinely. Joe Nocito -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of CrochiereSteve@aol.com Sent: Wednesday, June 30, 2004 9:23 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Cornea special stains Hi, Are there any "routine" special stains done on cornea transplants which have failed and been removed? I've started to get more than "one in a blue-moon" submitted to the lab and was wondering if there are any other stains to do other than H&E to show the cause for rejection. Thanks, Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kzhong888 <@t> yahoo.com Thu Jul 1 12:16:34 2004 From: kzhong888 <@t> yahoo.com (dfs dsaf) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] service manual for IEC CTD harris cryostat needed for MOHS Message-ID: <20040701171634.5154.qmail@web41603.mail.yahoo.com> Dear histonetters: thanks to Gareth and Irene for your help in responding to my last posting. I seem to be stuck in the land of broken cryostats. Our second cryostat just broke down, it is a IEC CTD Harris cryostat. I urgently need the SERVICE manual for it. If any one has a copy i would galdly pay for copy and mailing costs. thanks histonetters kirk --------------------------------- Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! From mcauliff <@t> umdnj.edu Thu Jul 1 16:15:28 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Parasympathetic and Sympathetic In-Reply-To: <002801c45eee$4a296230$21848e9d@hsc.net.ou.edu> References: <002801c45eee$4a296230$21848e9d@hsc.net.ou.edu> Message-ID: <40E47EF0.7040800@umdnj.edu> Hi Cameron: Sympathetics release epinephrine/norepinephrine, parasympathetics release acetylcholine. I don't know if there are any parasympathetics directly innervating heart muscle, most (at least the ones that I know of) innervate the SA and AV nodes to slow the heart rate. Geoff Cameron Hogan wrote: > I am trying to see parasympathetic and sympathetic innervation in cardiac tissue. What is the best way to distinguish each? Thanks for help! > >Cameron >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From paseifer <@t> MIT.EDU Thu Jul 1 13:26:04 2004 From: paseifer <@t> MIT.EDU (Philip Seifert) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Re: [IHCRG] CD31 on porcine In-Reply-To: References: Message-ID: <1088706364.40e4573c61409@webmail.mit.edu> Patsy, We've succesfully used Serotec's antibody to Porcine CD31 on paraffin and resin embedded tissues. Here are some research article references; Nugent HM, Groothuis A, Seifert P, Guerraro JL, Nedelman M, Mohanakumar T, Edelman ER. Perivascular endothelial implants inhibit intimal hyperplasia in a model of arteriovenous fistulae: a safety and efficacy study in the pig. Journal of Vascular Research. 2002 Nov-Dec;39(6):524-33. Nugent HM, Edelman ER. Endothelial implants provide long-term control of vascular repair in a porcine model of arterial injury. Journal of Surgical Research. 2001 Aug. 99(2): 228-34. Use pressure cooker-Antigen retrieval for 20 minutes (High pH is optimal though section adherance is a problem) or low temp water bath for 2-3 hours @ 80oC. I would suggest you use Dakocytomation CSAII or Envision+ for detection antigenicity is low for porcine CD31 in 10%NBF fixed tissues. Regards, Philip Seifert, MS, HTL(ASCP) Research Associate Massachusetts Institute of Technology Biomedical Engineering Center Histology Unit Building 16-336 77 Massachusetts Avenue Cambridge, MA 02139 USA E| paseifer@mit.edu ****************************** This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copy of this message,or any attachment , is strictly prohibited. If you have received this message in error, please notify the original sender immediately by telephone (617.253.1569) or by return email and delete this message, along with any attachments, from your computer. Thank you Quoting Patsy Ruegg : > Does anyone know of a CD31 or CD34 procedure that will work on ffpe pig > tissue? > Patsy > > > > ------------------------ Yahoo! Groups Sponsor --------------------~--> > Make a clean sweep of pop-up ads. Yahoo! Companion Toolbar. > Now with Pop-Up Blocker. Get it for free! > http://us.click.yahoo.com/L5YrjA/eSIIAA/yQLSAA/asSolB/TM > --------------------------------------------------------------------~-> > > > Yahoo! Groups Links > > <*> To visit your group on the web, go to: > http://groups.yahoo.com/group/IHCRG/ > > <*> To unsubscribe from this group, send an email to: > IHCRG-unsubscribe@yahoogroups.com > > <*> Your use of Yahoo! Groups is subject to: > http://docs.yahoo.com/info/terms/ > > > From RSRICHMOND <@t> aol.com Thu Jul 1 13:46:50 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Davidson's fixative Message-ID: <1a4.25cdd33e.2e15b61a@aol.com> >>1500 ml. 37% formaldehyde 2250 ml. Absolut [sic] alcohol [but I wouldn't pay the beverage tax for premium vodka!] ? 75 ml. glacial acetic acid 2250 ml. tap water eosin to color (not powder)<< This formula for Davidson's fixative isn't real close to the "modified Davidson's fixative" used by Moore and Barr (see my Web page about it for references): two parts 37% formaldehyde three parts 100% (or 95%) alcohol three parts tap water one part glacial acetic acid eosin to color As John Kiernan pointed out on this list a long time ago, a rather wide range of composition of this type of fixative will give about identical results. The proprietary fixatives mentioned are indeed secret formulas. It seems absurd both from a scientific and a financial viewpoint to use them, but who among us fathometh the wisdom of Management? In the surgical pathology laboratory, my major use for Davidson's fixative is as a clearing fixative to find mesenteric lymph nodes in colon resection specimens. My personal opinion is that use of clearing fixatives in this situation is a standard of care. In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From nwoolf <@t> ucsd.edu Thu Jul 1 14:09:06 2004 From: nwoolf <@t> ucsd.edu (Nigel Woolf) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <5.1.0.14.2.20040701120425.01f6a528@popmail.ucsd.edu> I have had a problem using Pap pens with immunofluorescent IHC: ink leaches out and the secondary antibodies stick giving high background. Any suggested pen for this application? Thanks! NW -------------- next part -------------- --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 From convmcm <@t> cc.usu.edu Thu Jul 1 14:34:41 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Thank you Herb In-Reply-To: Message-ID: <000c01c45fa2$74cb2eb0$4a737b81@Cygnus> Ditto that Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Walker Sent: Thursday, July 01, 2004 6:54 AM To: Histonet Subject: [Histonet] Thank you Herb A big thank you to Herb and anyone else that helped to fix the overload, Huzzah!!! Dave -- David Walker, MS Microbiology Staff Scientist CEHS/CSFN School of Pharmacy and Allied Health Sciences University of Montana Missoula Montana (406) 243-2225 Fax (406) 243-2807 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tynchas <@t> aol.com Thu Jul 1 18:05:34 2004 From: tynchas <@t> aol.com (Dave) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] In-situ film zymography Message-ID: Has anyone tackled the procedure in the subject line? We are having difficulty of lifting the frozen tissue sections off the film after incubation and staining with comassie blue. We then tried mounting the sections to slides instead of the film and laid the film on top of the slides but that was unsuccessful. Any suggestions would be greatly appreciated. Dave McClister, HTL Medical University of South Carolina Division of Cardiothoracic Surgery 114 Doughty St Charleston, SC 29425 843-876-5178 From caroline.stott <@t> anatomy.otago.ac.nz Thu Jul 1 15:41:35 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Von Kossa staining Message-ID: <5.2.1.1.0.20040702083543.02927ec0@anatomy.otago.ac.nz> Hi Tiffany, I have used this procedure many a time. If you need a control, immerse one slide in 4.5% citrate buffer for 30-40 minutes. Wash all slides in distilled water Flood slides with 5% silver nitrate Expose to bright sunlight, or UV light for 20 minutes, or a 60-watt lamp (about 4-5 inches from the slides) for 30-60 minutes Wash in several changes of distilled water Flood slides with 5% sodium thiosulphate for 2-3 minutes Counterstain with neutral red, safranine or van Gieson. Clear and mount. Hope that helps Caroline Caroline Stott Histology Service Unit University of Otago PO Box 913 Dunedin, New Zealand Ph (03) 479 7152 Fax (03) 479 7136 From JNocito <@t> Pathreflab.com Thu Jul 1 10:16:40 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] weird brownish stuff in AEC slides In-Reply-To: <2056946547.1088689963971.JavaMail.osg@spnode33> Message-ID: Rebekah, How old is your AEC and when do you make it up? I used to get tan-brown precipitate when I made up the AEC too far in advance. Also, have you changed lot numbers or vendors recently? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of SMITH,REBEKAH FELICIA Sent: Thursday, July 01, 2004 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weird brownish stuff in AEC slides I have yet another question, guys. I've been asking a lot of questions about blocking endogenous peroxidase,but now I have a new situation. I'm still using the ABC peroxidase method staining sheep placenta (formalin fixed, paraffin embedded) for eNOS and using AEC as a chromogen and gill's hematoxylin (blued with tap water and water with 2 or 3 drops of ammonia) as a counterstain. (btw, still blocking peroxidase with 30% peroxide in methanol, since my professors are away and haven't been able to order anything better) I have the lovely red and blue contrast in most parts of my slides, but I also am getting areas of brownish-black staining (kinda DAB colored, although I'm not using any DAB). I'm not exactly sure where the brown comes from this and whether its a problem with my AEC, my hematoxylin or something else. Anyone have any ideas? Thanks in advance, y'all have already been really helpful. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Thu Jul 1 17:01:16 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Manual for the vibratome Lancer series 1000 Message-ID: That would be the Vibratome Classic, the original model, sold through Lancer. A manual will be sent from the factory to the address you have provided below. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hernan Aldana Marcos Sent: Thursday, July 01, 2004 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual for the vibratome Lancer series 1000 Does anybody have the manual for the old vibratome Lancer Series 1000? Thanks in advance. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JColCLEFA <@t> aol.com Thu Jul 1 11:09:13 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Corneas Message-ID: <1db.255922ff.2e159129@aol.com> We usually do a PAS/ lt green (fungus ctl) for all corneas. We found an amebic cyst once. (pond swimming after xplant=bad idea. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vbaker60 <@t> yahoo.com Thu Jul 1 17:24:50 2004 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Hydrophobic Pens In-Reply-To: <5.1.0.14.2.20040701120425.01f6a528@popmail.ucsd.edu> Message-ID: <20040701222450.72888.qmail@web50107.mail.yahoo.com> I gave up on those pens about 3 years ago. Now I use a 2X2 parafilm strip cut in half length wise and after applying antibody or reagent lay the parafilm over the slide, covering the tissue/cells. I have no leaks, and no dried out samples. Vikki Baker Institute for Cancer Prevention Valhalla, NY 10595 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From JNocito <@t> Pathreflab.com Thu Jul 1 10:07:18 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Cornea special stains In-Reply-To: <45.fa0f236.2e14cf92@aol.com> Message-ID: Steven, Once in a while, a doctor orders a PAS for Fungus, but we don't perform these routinely. Joe Nocito -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of CrochiereSteve@aol.com Sent: Wednesday, June 30, 2004 9:23 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Cornea special stains Hi, Are there any "routine" special stains done on cornea transplants which have failed and been removed? I've started to get more than "one in a blue-moon" submitted to the lab and was wondering if there are any other stains to do other than H&E to show the cause for rejection. Thanks, Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paseifer <@t> MIT.EDU Thu Jul 1 13:26:04 2004 From: paseifer <@t> MIT.EDU (Philip Seifert) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Re: [IHCRG] CD31 on porcine In-Reply-To: References: Message-ID: <1088706364.40e4573c61409@webmail.mit.edu> Patsy, We've succesfully used Serotec's antibody to Porcine CD31 on paraffin and resin embedded tissues. Here are some research article references; Nugent HM, Groothuis A, Seifert P, Guerraro JL, Nedelman M, Mohanakumar T, Edelman ER. Perivascular endothelial implants inhibit intimal hyperplasia in a model of arteriovenous fistulae: a safety and efficacy study in the pig. Journal of Vascular Research. 2002 Nov-Dec;39(6):524-33. Nugent HM, Edelman ER. Endothelial implants provide long-term control of vascular repair in a porcine model of arterial injury. Journal of Surgical Research. 2001 Aug. 99(2): 228-34. Use pressure cooker-Antigen retrieval for 20 minutes (High pH is optimal though section adherance is a problem) or low temp water bath for 2-3 hours @ 80oC. I would suggest you use Dakocytomation CSAII or Envision+ for detection antigenicity is low for porcine CD31 in 10%NBF fixed tissues. Regards, Philip Seifert, MS, HTL(ASCP) Research Associate Massachusetts Institute of Technology Biomedical Engineering Center Histology Unit Building 16-336 77 Massachusetts Avenue Cambridge, MA 02139 USA E| paseifer@mit.edu ****************************** This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copy of this message,or any attachment , is strictly prohibited. If you have received this message in error, please notify the original sender immediately by telephone (617.253.1569) or by return email and delete this message, along with any attachments, from your computer. Thank you Quoting Patsy Ruegg : > Does anyone know of a CD31 or CD34 procedure that will work on ffpe pig > tissue? > Patsy > > > > ------------------------ Yahoo! Groups Sponsor --------------------~--> > Make a clean sweep of pop-up ads. Yahoo! Companion Toolbar. > Now with Pop-Up Blocker. Get it for free! > http://us.click.yahoo.com/L5YrjA/eSIIAA/yQLSAA/asSolB/TM > --------------------------------------------------------------------~-> > > > Yahoo! Groups Links > > <*> To visit your group on the web, go to: > http://groups.yahoo.com/group/IHCRG/ > > <*> To unsubscribe from this group, send an email to: > IHCRG-unsubscribe@yahoogroups.com > > <*> Your use of Yahoo! Groups is subject to: > http://docs.yahoo.com/info/terms/ > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Thu Jul 1 11:53:32 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] CD31 on porcine Message-ID: Does anyone know of a CD31 or CD34 procedure that will work on ffpe pig tissue? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JColCLEFA <@t> aol.com Thu Jul 1 11:09:13 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Corneas Message-ID: <1db.255922ff.2e159129@aol.com> We usually do a PAS/ lt green (fungus ctl) for all corneas. We found an amebic cyst once. (pond swimming after xplant=bad idea. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paseifer <@t> MIT.EDU Thu Jul 1 13:26:04 2004 From: paseifer <@t> MIT.EDU (Philip Seifert) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Re: [IHCRG] CD31 on porcine In-Reply-To: References: Message-ID: <1088706364.40e4573c61409@webmail.mit.edu> Patsy, We've succesfully used Serotec's antibody to Porcine CD31 on paraffin and resin embedded tissues. Here are some research article references; Nugent HM, Groothuis A, Seifert P, Guerraro JL, Nedelman M, Mohanakumar T, Edelman ER. Perivascular endothelial implants inhibit intimal hyperplasia in a model of arteriovenous fistulae: a safety and efficacy study in the pig. Journal of Vascular Research. 2002 Nov-Dec;39(6):524-33. Nugent HM, Edelman ER. Endothelial implants provide long-term control of vascular repair in a porcine model of arterial injury. Journal of Surgical Research. 2001 Aug. 99(2): 228-34. Use pressure cooker-Antigen retrieval for 20 minutes (High pH is optimal though section adherance is a problem) or low temp water bath for 2-3 hours @ 80oC. I would suggest you use Dakocytomation CSAII or Envision+ for detection antigenicity is low for porcine CD31 in 10%NBF fixed tissues. Regards, Philip Seifert, MS, HTL(ASCP) Research Associate Massachusetts Institute of Technology Biomedical Engineering Center Histology Unit Building 16-336 77 Massachusetts Avenue Cambridge, MA 02139 USA E| paseifer@mit.edu ****************************** This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copy of this message,or any attachment , is strictly prohibited. If you have received this message in error, please notify the original sender immediately by telephone (617.253.1569) or by return email and delete this message, along with any attachments, from your computer. Thank you Quoting Patsy Ruegg : > Does anyone know of a CD31 or CD34 procedure that will work on ffpe pig > tissue? > Patsy > > > > ------------------------ Yahoo! Groups Sponsor --------------------~--> > Make a clean sweep of pop-up ads. Yahoo! Companion Toolbar. > Now with Pop-Up Blocker. Get it for free! > http://us.click.yahoo.com/L5YrjA/eSIIAA/yQLSAA/asSolB/TM > --------------------------------------------------------------------~-> > > > Yahoo! Groups Links > > <*> To visit your group on the web, go to: > http://groups.yahoo.com/group/IHCRG/ > > <*> To unsubscribe from this group, send an email to: > IHCRG-unsubscribe@yahoogroups.com > > <*> Your use of Yahoo! Groups is subject to: > http://docs.yahoo.com/info/terms/ > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:40 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 1 18:09:11 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Hydrophobic Pens Message-ID: <1609580574.1088723351482.JavaMail.osg@spnode33> I've been using Zymed pap pen (the thin kind) and usually it works fine. The only thing is if you're using a chromogen that can't be in xylene or alcohol (like AEC), if you put it on too thick, the pap pen marks might not rinse off that well.(I've been using AEC as of late,and I've found I can still use the pap pen, I just have to be careful of how much and rinse a lot with a couple changes of water after the AEC, or use a wash bottle to rinse.) If you're using DAB, the marks should disappear when you go up to xylene again. On Thu Jul 01 15:09:06 EDT 2004, Nigel Woolf wrote: > I have had a problem using Pap pens with immunofluorescent IHC: > ink leaches out and the secondary antibodies stick giving high > background. Any suggested pen for this application? Thanks! NW > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.713 / Virus Database: 469 - Release Date: 6/30/2004 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jul 1 18:12:06 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Tracking Outgoing Blocks and Slides Message-ID: Paula, We are on Meditech. When a block(s) or slide(s) are sent out the person sending out the materials adds an electronic marker (we use "POC" for pathology outside consult) into the marker data section of the pathology report. When the materials are returned the marker is removed. On a regular basis, usually weekly the lead transcriptionist runs a search for all outstanding POC markers which are greater out than two weeks and those not having the materials returned are followed up with a phone call. Works very well for us. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Paula Lucas [mailto:plucas@biopath.org] Sent: Thursday, July 01, 2004 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking Outgoing Blocks and Slides What type of tracking system do you use for blocks and slides that go out to the pathologists or other institutions for consultations and such? I want to make sure that whatever is going out comes back. We need a better plan than our current method. Thanks in advance. Paula Lucas Bio-Path Medial Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Thu Jul 1 20:48:40 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] I have turned the list back to automatic Message-ID: All, Things look quiet so I an resuming automatic processing of list messages. If I detect any problems I will resume manual control. There may be a few double messages that came out this afternoon, I am hoping that all messages don't get doubled up. If they continue after 22:00 Thursday, I will take the list back to manual control in the morning. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From herb.hagler <@t> UTSouthwestern.edu Thu Jul 1 22:58:44 2004 From: herb.hagler <@t> UTSouthwestern.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Back to emergency moderation mode Message-ID: <1CF8B620-CBDC-11D8-8396-000A95D9F7BA@UTSouthwestern.edu> Double messages again, darn.... back to manual mode until we get some more time to fix the server.. Herb From barbara.bublava <@t> meduniwien.ac.at Fri Jul 2 01:12:58 2004 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] silver stain References: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111358@mius2.medlan.cam.ac.uk> Message-ID: <00ba01c45ffb$9f7dac60$1201a8c0@GERICHTS9XOZZ8> Thank you all for your replys! My question was more of theoretical interest. We do no silverstains in routine but maybe it could happen that there is one ordered from an already processed specimen. I just wanted to know if there would be a problem with not washed specimens Thanks and regards to all Barbara, Vienna From mab70 <@t> medschl.cam.ac.uk Fri Jul 2 02:01:55 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Steve Slap Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211135A@mius2.medlan.cam.ac.uk> If anone else has a sympathy message for Lisa, Jeremy and Steve's Mum, please email me before 4.00pm UK time today. I will add any messages to my list at that time. I will be posting these off tomorrow. By the way, UK time is about 5 hours ahead of New York if that helps. Best wishes Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR From barbara.bublava <@t> meduniwien.ac.at Fri Jul 2 01:12:58 2004 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] silver stain References: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111358@mius2.medlan.cam.ac.uk> Message-ID: <00ba01c45ffb$9f7dac60$1201a8c0@GERICHTS9XOZZ8> Thank you all for your replys! My question was more of theoretical interest. We do no silverstains in routine but maybe it could happen that there is one ordered from an already processed specimen. I just wanted to know if there would be a problem with not washed specimens Thanks and regards to all Barbara, Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Fri Jul 2 02:01:55 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Steve Slap Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211135A@mius2.medlan.cam.ac.uk> If anone else has a sympathy message for Lisa, Jeremy and Steve's Mum, please email me before 4.00pm UK time today. I will add any messages to my list at that time. I will be posting these off tomorrow. By the way, UK time is about 5 hours ahead of New York if that helps. Best wishes Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LettJ <@t> ent.wustl.edu Fri Jul 2 08:11:18 2004 From: LettJ <@t> ent.wustl.edu (Lett, Jaclynn) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] replying to digests Message-ID: <8153997545DC1A4DB92AE75F5DC345001FEB17@EXCHANGE.wusm-pcf.wustl.edu> In light of the problems we have just experienced, may I make a plea that users not reply to a digest posting as a whole? Those of us who use digest mode end up having to scroll through an entire digest message to find the few postings in which one is interested. "Reply" but please delete everything before and after the posting to which you are replying. Not doing so ridiculously increases the length of the digest. Thank you kindly, JM Lett Senior Research Technician, EM Core Facility Washington University School of Medicine Department of Otolaryngology 660 South Euclid Ave., Box 8115 St. Louis, MO 63110 email: lettj@ent.wustl.edu voice: 314-747-7257 fax: 314-747-7230 From Terry.Marshall <@t> rothgen.nhs.uk Fri Jul 2 08:31:12 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Davidson's fixative Message-ID: Bob Richmond said: "In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it." Do you really. really believe that Bob? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: 01 July 2004 19:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Davidson's fixative >>1500 ml. 37% formaldehyde 2250 ml. Absolut [sic] alcohol [but I wouldn't pay the beverage tax for premium vodka!] ? 75 ml. glacial acetic acid 2250 ml. tap water eosin to color (not powder)<< This formula for Davidson's fixative isn't real close to the "modified Davidson's fixative" used by Moore and Barr (see my Web page about it for references): two parts 37% formaldehyde three parts 100% (or 95%) alcohol three parts tap water one part glacial acetic acid eosin to color As John Kiernan pointed out on this list a long time ago, a rather wide range of composition of this type of fixative will give about identical results. The proprietary fixatives mentioned are indeed secret formulas. It seems absurd both from a scientific and a financial viewpoint to use them, but who among us fathometh the wisdom of Management? In the surgical pathology laboratory, my major use for Davidson's fixative is as a clearing fixative to find mesenteric lymph nodes in colon resection specimens. My personal opinion is that use of clearing fixatives in this situation is a standard of care. In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LESCHUKC <@t> trinity-health.org Fri Jul 2 08:37:53 2004 From: LESCHUKC <@t> trinity-health.org (Carmen Leschuk) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] HELP! Removing tissue from heat-fixed slides. Message-ID: I have a problem and need some quick advice! My Pathologist was going to send a liver bx block out for copper studies, but my tech accidently cut extra sections on the block and there is no tissue left. The paraffin embedded tissue is needed for the copper study. The unstained liver bx sections were also placed on charged slides and heat-fixed. Does ANYONE know of a way I can get these sections off of the slide, if I can get them off, I could centrifuge and place the button in paraffin. Please let me know ASAP! Thanks! Carmen Leschuk, HT (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 From hagler.herb <@t> pathology.swmed.edu Fri Jul 2 08:43:45 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Please help by doing your own subscribe and unsubscribes via the web site Message-ID: Please help.... If you wish to subscribe or unsubscribe from the list can you please go to the web site and do this yourself. You recently received a message giving the link, the name you are subscribed under and your password. To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet I am buried in trying to just keep the messages flowing and having difficulty finding the time to manually do the subscribes and unsubscribes at this time. Thanks, Herb From barbara.bublava <@t> meduniwien.ac.at Fri Jul 2 01:12:58 2004 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] silver stain References: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111358@mius2.medlan.cam.ac.uk> Message-ID: <00ba01c45ffb$9f7dac60$1201a8c0@GERICHTS9XOZZ8> Thank you all for your replys! My question was more of theoretical interest. We do no silverstains in routine but maybe it could happen that there is one ordered from an already processed specimen. I just wanted to know if there would be a problem with not washed specimens Thanks and regards to all Barbara, Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LESCHUKC <@t> trinity-health.org Fri Jul 2 08:37:53 2004 From: LESCHUKC <@t> trinity-health.org (Carmen Leschuk) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] HELP! Removing tissue from heat-fixed slides. Message-ID: I have a problem and need some quick advice! My Pathologist was going to send a liver bx block out for copper studies, but my tech accidently cut extra sections on the block and there is no tissue left. The paraffin embedded tissue is needed for the copper study. The unstained liver bx sections were also placed on charged slides and heat-fixed. Does ANYONE know of a way I can get these sections off of the slide, if I can get them off, I could centrifuge and place the button in paraffin. Please let me know ASAP! Thanks! Carmen Leschuk, HT (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Fri Jul 2 02:01:55 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Steve Slap Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211135A@mius2.medlan.cam.ac.uk> If anone else has a sympathy message for Lisa, Jeremy and Steve's Mum, please email me before 4.00pm UK time today. I will add any messages to my list at that time. I will be posting these off tomorrow. By the way, UK time is about 5 hours ahead of New York if that helps. Best wishes Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Jul 2 08:31:12 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Davidson's fixative Message-ID: Bob Richmond said: "In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it." Do you really. really believe that Bob? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: 01 July 2004 19:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Davidson's fixative >>1500 ml. 37% formaldehyde 2250 ml. Absolut [sic] alcohol [but I wouldn't pay the beverage tax for premium vodka!] ? 75 ml. glacial acetic acid 2250 ml. tap water eosin to color (not powder)<< This formula for Davidson's fixative isn't real close to the "modified Davidson's fixative" used by Moore and Barr (see my Web page about it for references): two parts 37% formaldehyde three parts 100% (or 95%) alcohol three parts tap water one part glacial acetic acid eosin to color As John Kiernan pointed out on this list a long time ago, a rather wide range of composition of this type of fixative will give about identical results. The proprietary fixatives mentioned are indeed secret formulas. It seems absurd both from a scientific and a financial viewpoint to use them, but who among us fathometh the wisdom of Management? In the surgical pathology laboratory, my major use for Davidson's fixative is as a clearing fixative to find mesenteric lymph nodes in colon resection specimens. My personal opinion is that use of clearing fixatives in this situation is a standard of care. In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Fri Jul 2 08:43:45 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Please help by doing your own subscribe and unsubscribes via the web site Message-ID: Please help.... If you wish to subscribe or unsubscribe from the list can you please go to the web site and do this yourself. You recently received a message giving the link, the name you are subscribed under and your password. To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet I am buried in trying to just keep the messages flowing and having difficulty finding the time to manually do the subscribes and unsubscribes at this time. Thanks, Herb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Jul 2 09:09:52 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Re: Davidson's fixative Message-ID: <86.fc22245.2e16c6b0@aol.com> Dr Terry L Marshall, Consultant Pathologist, Rotherham General Hospital, South Yorkshire, England replies to my comment about the usefulness of clearing fixatives in staging colon cancer: >>"In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it." Do you really, really believe that Bob?<< I'm not sure that this is an evidence-based statement, but the chemotherapy of colon cancer is beginning to make some real advances after a very long period of stagnation, and I feel like going along with what the oncologists are asking for. - Anyway, I did say "maybe". Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From RSRICHMOND <@t> aol.com Fri Jul 2 09:09:52 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Re: Davidson's fixative Message-ID: <86.fc22245.2e16c6b0@aol.com> Dr Terry L Marshall, Consultant Pathologist, Rotherham General Hospital, South Yorkshire, England replies to my comment about the usefulness of clearing fixatives in staging colon cancer: >>"In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it." Do you really, really believe that Bob?<< I'm not sure that this is an evidence-based statement, but the chemotherapy of colon cancer is beginning to make some real advances after a very long period of stagnation, and I feel like going along with what the oncologists are asking for. - Anyway, I did say "maybe". Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flemons <@t> bhset.org Fri Jul 2 10:12:49 2004 From: flemons <@t> bhset.org (Fran Lemons) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Coverslippers and slide labelers Message-ID: I am interested in everyone's opinion of their favorite automatic coverslippers and also automatic slide labelers. I've been given a chance to make a wish list, and I have my favorites, but I want to make sure there's not something even better out there. Thanks in advance Fran Walker Histology Technical Specialist Baptist Hospital of East TN, Knoxville From Terry.Marshall <@t> rothgen.nhs.uk Fri Jul 2 10:21:35 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] RE: Davidson's fixative Message-ID: Sorry this is rather off-topic in as much as it is medical. I am bothered by the reliance on rather "fussy" results to determine therapy. In the case of LN metastases, we can surely improve the yield of positives by greater effort, time, money and vigour in the search. However, it is a practical impossibility to be exhaustive in our examination of nodes. Do we get a better yield by finding a 2mm node than by cutting multiple blocks of a 8mm node? What about levels? How about immunochemistry? We are now being told that there is an entity of micrometastases, that is to say, nodal metastases that are "isolated" and less than 2mm diameter. These, we are told, are to be disregarded. Logically, if we see one, we should cut levels to see if they expand to more than 2mm. I find this all to be utter garbage, and I don't mean (just) my post:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: 02 July 2004 15:10 To: Marshall Terry Dr, Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: Re: Davidson's fixative Dr Terry L Marshall, Consultant Pathologist, Rotherham General Hospital, South Yorkshire, England replies to my comment about the usefulness of clearing fixatives in staging colon cancer: >>"In colon cancer positive lymph nodes are sometimes very small, and finding even one positive lymph node often results in a patient's receiving postoperative chemotherapy and maybe having their life saved by it." Do you really, really believe that Bob?<< I'm not sure that this is an evidence-based statement, but the chemotherapy of colon cancer is beginning to make some real advances after a very long period of stagnation, and I feel like going along with what the oncologists are asking for. - Anyway, I did say "maybe". Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From mab70 <@t> medschl.cam.ac.uk Fri Jul 2 11:18:29 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] website Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111360@mius2.medlan.cam.ac.uk> If anyone hears that a website is set up about Steve Slap, please let me know, so I can send my compilation of messages of condolence to it. The response has been moving and I hope reading these will bring comfort to Steve's loved ones. Thank you histonet family. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR From herme013 <@t> umn.edu Fri Jul 2 12:10:13 2004 From: herme013 <@t> umn.edu (Yves Heremans) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] luciferase antibody Message-ID: <200407021710.i62HADWB025749@trojan.software.umn.edu> Hi All, Does anyone know a good antibody for detection of firefly luciferase in formalin-fixed paraffin embedded tissue ? Or is detection limited to frozen tissue ? Yves   From histo <@t> bthosp.com Fri Jul 2 12:15:35 2004 From: histo <@t> bthosp.com (O'Brien, Sue) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Use of polarization microscopy in the diagnosis of pneumocystis p neumonia Message-ID: <3653750027C5D6119D0600508BB89A9A0AC081@MAIL_SERVER> I was wondering if anyone else had tried Dr. Teisa An's method (published in the March 2004 issue of Arch. Pathol. Lab. Med.)? It utilizes pepsin, and then the PCP can be visualized via polarization. For those that have tried it, what do you think? It is very easy to do the procedure, but the results seem a little different (for someone used to looking at silver stained material). Thank-you, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 From azdudley <@t> hotmail.com Fri Jul 2 13:17:01 2004 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] repeats Message-ID: is anyone else having trouble receiving multiple emails, I am getting 3 and 4 of each message? maybe something wrong with my mail server? anita dudley, mobile alabama _________________________________________________________________ Check out the latest news, polls and tools in the MSN 2004 Election Guide! http://special.msn.com/msn/election2004.armx From victor <@t> pathology.washington.edu Fri Jul 2 14:15:07 2004 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] CAP and Computer Training Message-ID: <40E5B43B.4010203@pathology.washington.edu> We have been going round and round over this. Someone has said that CAP requires semi-annual refresher training on the computer system, but we can't find any documentation. Help!! Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax From RSRICHMOND <@t> aol.com Fri Jul 2 15:15:04 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] copper in tissue (removing tissue from heat fixed slides) Message-ID: <15b.38fddcd8.2e171c48@aol.com> Carmen Leschuk inquires whether it's possible to send sections for quantitative copper determination, when the paraffin block has been exhausted. Mayo Medical Laboratories (who probably does more of these than anybody) requires 5 to 10 mm of paraffin-embedded liver tissue, so I'm afraid you're out of luck. Liver copper needs to be quantitated in Wilson's disease, a complex genetic disease that manifests in many different ways, sometimes as chronic liver disease with cirrhosis, sometimes as a brain disease. I'm afraid the biopsy will have to be repeated, or the diagnosis made in some other way. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From neuroant <@t> hotmail.com Fri Jul 2 15:41:23 2004 From: neuroant <@t> hotmail.com (Ant S.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] repeats Message-ID: No, I am also having problems with repeats. I've gotten more then 30 repeats of some of the e-mails. Anyone have any idea what's up? Antoinette Swensson Univeristy of Washington/Harborview Medical Center Neuropathology 325 9th Ave MS 359-791 Seattle, WA 98104 (206) 731-3910 >From: "anita dudley" >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] repeats >Date: Fri, 02 Jul 2004 18:17:01 +0000 > >is anyone else having trouble receiving multiple emails, I am >getting 3 and 4 of each message? maybe something wrong with my mail >server? anita dudley, mobile alabama > >_________________________________________________________________ >Check out the latest news, polls and tools in the MSN 2004 Election >Guide! http://special.msn.com/msn/election2004.armx > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]MSN Toolbar provides one-click access to Hotmail from any Web page FREE download! References 1. http://g.msn.com/8HMBENUS/2740??PS=47575 From Jason.Wiese <@t> med.va.gov Fri Jul 2 17:01:14 2004 From: Jason.Wiese <@t> med.va.gov (Wiese, Jason VHAROS) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Help Message-ID: Hello: My name is Jason E. Wiese, and I am an ASCP certified HT. I work for the Department of Veteran Affairs in Roseburg Oregon. I recently found that my position description was written in February of 1968. I have been giving permission to update, or rather, rewrite my PD, but am needing help. I have never been on the histonet before, and am not sure how it works. I am looking for some fellow Histotechnologists who may work for the VA, and may be able to help me with an electronic copy of a PD from which to work from. I am a lead tech, as I run my own lab, and am the sole tech. However, the only supervising I do is training a back-up tech for when I am on vacations. I would greatly appreciate any info that anyone could give me about who I might be able to contact regarding this issue. Thank You! Jason Jason E. Wiese, HT(ASCP) Roseburg, Oregon VAMC Phone: (541)440-1000 ext. 44751 Email: jason.wiese@med.va.gov From Jason.Wiese <@t> med.va.gov Fri Jul 2 17:08:42 2004 From: Jason.Wiese <@t> med.va.gov (Wiese, Jason VHAROS) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] FW: Help Message-ID: This one should go through, as now I have subscribed to be a member... Thanks Again!! Jason > -----Original Message----- > From: Wiese, Jason VHAROS > Sent: Friday, July 02, 2004 3:01 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Help > > Hello: > > My name is Jason E. Wiese, and I am an ASCP certified HT. I work for the > Department of Veteran Affairs in Roseburg Oregon. I recently found that > my position description was written in February of 1968. I have been > giving permission to update, or rather, rewrite my PD, but am needing > help. I have never been on the histonet before, and am not sure how it > works. I am looking for some fellow Histotechnologists who may work for > the VA, and may be able to help me with an electronic copy of a PD from > which to work from. I am a lead tech, as I run my own lab, and am the > sole tech. However, the only supervising I do is training a back-up tech > for when I am on vacations. I would greatly appreciate any info that > anyone could give me about who I might be able to contact regarding this > issue. > > Thank You! > Jason > > > > Jason E. Wiese, HT(ASCP) > Roseburg, Oregon VAMC > Phone: (541)440-1000 ext. 44751 > Email: jason.wiese@med.va.gov > > > From JNocito <@t> Pathreflab.com Fri Jul 2 17:23:42 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] CAP and Computer Training In-Reply-To: <40E5B43B.4010203@pathology.washington.edu> Message-ID: Victor, I'm preparing for my CAP for the Fall and I don't see anything mentioned about computer training. If there is something, please let me know. I must be missing it. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victor Tobias Sent: Friday, July 02, 2004 2:15 PM To: Histonet Subject: [Histonet] CAP and Computer Training We have been going round and round over this. Someone has said that CAP requires semi-annual refresher training on the computer system, but we can't find any documentation. Help!! Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MinHan.Tan <@t> vai.org Fri Jul 2 17:32:33 2004 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Storage of slides and tissue arrays Message-ID: <74D0F0AB07F2E647A02D839ED79520F9B488D9@VAIEXCH02.vai.org> Good morning, I'd like to enquire if anyone stores their slides at 4 deg C or -20 deg C. We have some paraffin embedded slides of a tissue array from another facility which the pathologist requested that we store at 4 deg C or -20 deg C. I understand slides may not work as well after some time for IHC, but I've never heard of storing at 4 deg C / -20 - does it affect the slides? What about condensation and all that? Thanks. Min-Han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. From MinHan.Tan <@t> vai.org Fri Jul 2 17:32:33 2004 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Storage of slides and tissue arrays Message-ID: <74D0F0AB07F2E647A02D839ED79520F9B488D9@VAIEXCH02.vai.org> Good morning, I'd like to enquire if anyone stores their slides at 4 deg C or -20 deg C. We have some paraffin embedded slides of a tissue array from another facility which the pathologist requested that we store at 4 deg C or -20 deg C. I understand slides may not work as well after some time for IHC, but I've never heard of storing at 4 deg C / -20 - does it affect the slides? What about condensation and all that? Thanks. Min-Han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jhabecke <@t> seattlecca.org Fri Jul 2 20:54:22 2004 From: jhabecke <@t> seattlecca.org (Randolph-Habecker, Julie) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Autofluorescence from RBC Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B9484EAB50@wala01.seattlecca.org> Hey folks, We are doing immunofluorescence on some paraformaldehyde-fixed paraffin-embedded mouse lung that has been subjected to chemical injury. As a result there is a great deal of hemorrhage. We have been using some techniques to reduce autofluorescence from a paper suggested on histonet (Baschong et al, JHC 49: 1565, 2001) however, the RBC are still very fluorescent. Any other suggestions????? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Experimental Histopathology Shared Resources Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, G1-300 PO Box 19023 Seattle, WA 98109-1024 Tel: (206) 288-1187 FAX: (206) 288-1345 jhabecke@fhcrc.org This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From RSRICHMOND <@t> aol.com Sat Jul 3 13:18:18 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] pneumocystis by polarization Message-ID: <98.edae86b.2e18526a@aol.com> Sue O'Brien, Histology Supervisor, at Burdette Tomlin Memorial Hospital, Cape May Court House, New Jersey, asks: >>I was wondering if anyone else had tried Dr. Teisa An's method (published in the March 2004 issue of Arch. Pathol. Lab. Med.)? It utilizes pepsin, and then the PCP can be visualized via polarization. For those that have tried it, what do you think? It is very easy to do the procedure, but the results seem a little different (for someone used to looking at silver stained material).<< The article is "The use of polarization microscopy in the diagnosis of pneumocystis pneumonia" by Teisa An MD and Pam Tabaczka BS(MT) in the pathology department at Harper University Hospital in Detroit MI (Dr. An's e-mail is tan at dmc.org.), in Arch Pathol Lab Med 2004;128:163-4. They write "When tissue sections that had been treated with pepsin and stained with hematoxylin only were examined under polarized light, the cyst forms of P carinii were observed as being clearly birefringent and were as numerous as when a Grocott methenamine silver-stained slide was examined." This article is a good example of why I get the Archives because the College of American Pathologists sends it to me, but usually let it accumulate on top of the previous month's number. The authors report exactly one case, without even noting whether a pneumocystis control slide was positive, how they applied the pepsin, or what hematoxylin they used. They offer no relevant references, and no explanation of how the method might work. They don't mention that the present name of the human pathogen is Pneumocystis jiroveci. If the Archives is actually a refereed journal, then taunts about the umpire's seeing-eye dog are definitely in order. Still, it couldn't hurt to try the method, but it's hardly ready for prime time. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From cytch7 <@t> cox-internet.com Fri Jul 2 20:31:18 2004 From: cytch7 <@t> cox-internet.com (Valerie Biendara) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] CAP and Computer Training In-Reply-To: Message-ID: <20040703013142.KIWX14781.fe6@Valerie> See CAP Checklist, Laboratory General GEN.44500 Phase II N/A YES NO Is there documentation that all users of the computer system receive adequate training initially, after system modification, and after installation of a new system? COMMENTARY: The people who interact with the computer system must initially be taught how to use a new system and must be trained on modifications to an existing system. There must be documentation of these training activities. Victor, I'm preparing for my CAP for the Fall and I don't see anything mentioned about computer training. If there is something, please let me know. I must be missing it. We have been going round and round over this. Someone has said that CAP requires semi-annual refresher training on the computer system, but we can't find any documentation. Help!! Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JColCLEFA <@t> aol.com Sun Jul 4 09:07:56 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] restaining after HX, Kathy Abel, Biocare Message-ID: <31.49f17f6f.2e19693c@aol.com> we routinely stain for IHC slides which have not only been dunked in HX, but which have been stained, coverslipped and stored for a number of months as routine H&E's We don't usually have an issue with reactivity. (this happens mostly on prostate biopsies, and we use a pretty thorough antigen retrieval for these and all slides, except a few antibodies whose epitopes don't handle the heat very well.) A short hx bath won't affect anything in my opinion, I would not, however, decolorize the slides in acid alcohol, for us strong acid treatments (not the pH6.0 of the retrieval buffer) like decal or some decolorizing processes tend to reduce reactivity of certain antibodies (Pax 5 for instance) Does anybody know if Kathy Abel is out of the office and when she might return? I must have left her about a thousand messages- HAHA Biocare Medical Is a great company and even though I only spend like 10 bucks a month with them they still treat me like a VIP- I think their science and quality is among the best. I thought about their stainer and will consider it when when my present contract is up. Post another note after you use the thing for a while longer, will ya? From carl.hobbs <@t> kcl.ac.uk Sun Jul 4 14:04:07 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] re RBC autofluorescence Message-ID: <006801c461f9$ae97ee40$d8949a51@home> Difficult for me too......only success I've had with cell monolayers is to post stain using std Sudan black...then differentiating out until only your marker is fluorescing...fiddly. Can't remember the original article. It worked tho. Loads of people onsite can be definite, I'm sure. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 From hagler.herb <@t> pathology.swmed.edu Sun Jul 4 20:37:04 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Duplicate messages Message-ID: We think that we have identified the problem. There was a computer on the internet, maybe a subscriber which was bouncing all of the histonet messages back to our server. This put it in an infinite loop of delivering the same message over and over again. There were also several people who were using the vacation auto reply. Only one person was bouncing those back to the server. We may still have some trouble and if more repeats come in we will go back to manual operation and continue looking for the internet address that is causing the disruption via the repeating messages. Hope everyone in the US had a happy holiday. Herb From kerrie.thomson.kt <@t> bayer-ag.de Sun Jul 4 22:45:59 2004 From: kerrie.thomson.kt <@t> bayer-ag.de (kerrie.thomson.kt@bayer-ag.de) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Kerrie Thomson/MELB/AU/BAYER is out of the office. Message-ID: I will be out of the office starting 05/07/2004 and will not return until 12/07/2004. I will respond to your message when I return. From bruyntjes <@t> voeding.tno.nl Tue Jul 6 01:02:05 2004 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] (no subject) Message-ID: <3B070848E7C2204F9DEB8BCFD76772800210D689@ntexch1.voeding.tno.nl> Curious One day repetition after repetition, yesterday no mails at all? Joost Bruyntjes This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From hagler.herb <@t> pathology.swmed.edu Tue Jul 6 09:53:01 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Test post - Delete me Message-ID: <2D7ECD05-CF5C-11D8-9394-000A95D9F7BA@pathology.swmed.edu> Just a test posting. Please delete Checking for double messages Thanks, Herb From nancy.troiano <@t> yale.edu Tue Jul 6 10:11:05 2004 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] BMP2 antibody Message-ID: <5.2.1.1.2.20040706110929.00b9f398@email.med.yale.edu> Help! Does anyone know of a source of anti-mouse BMP2 (bone morphogenic protein) appropriate to use on FFPE or frozen mouse tissue? Thanks! From mgorin <@t> umich.edu Tue Jul 6 11:22:54 2004 From: mgorin <@t> umich.edu (mgorin@umich.edu) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] nissl stain Message-ID: <1089130974.40ead1de8c460@mail.umich.edu> sry guys i am new at this. but can somone tell me if nissl, when doen to brain tissue, will only stain neurons? will it stain glial cells? also, how does nissl work. wat bodies does it stain. thanks. -mike- UMICH- Ann Arbor From Robert.Lott <@t> bhsala.com Tue Jul 6 11:25:07 2004 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Tris-EDTA Recipe, pH 9.0 for Antigen Retrieval Message-ID: <35B6C610DD1DD311B1FA0008C791400407E145D7@gobexchm3.bhsala.com> Has anyone got a recipe for Tris-EDTA, ph 9.0 ? Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. From HornHV <@t> archildrens.org Tue Jul 6 11:28:40 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] knives and scissors with disposable blades? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6322B8DC@EMAIL.archildrens.org> We have a new pathologist fresh out of school. She tells me the place she was a resident (somewhere in Texas) had long knives and scissors with disposable blades. Can any one help me out with this. I have searched the internet but did not come up with anything. Thanks. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Robert.Lott <@t> bhsala.com Tue Jul 6 11:48:47 2004 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Recipe for Tris-EDTA, pH 9.0 for Antigen Retreival Message-ID: <35B6C610DD1DD311B1FA0008C791400407E145D8@gobexchm3.bhsala.com> Anyone got a recipe for the solution above? How do you dissolve the EDTA in the solution? Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. From pruegg <@t> colobio.com Tue Jul 6 11:55:40 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] BMP2 antibody In-Reply-To: <5.2.1.1.2.20040706110929.00b9f398@email.med.yale.edu> Message-ID: Santa Cruz usually has the market on all the BMP's unless someone else has entered in the last couple of years. SC is not very helpful in providing info for use on ffpe tissue, but I have gotten BMP's to work on formic acid decalcified zinc formalin pe tissues, usally with pepsin digestion. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nancy W. Troiano Sent: Tuesday, July 06, 2004 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BMP2 antibody Help! Does anyone know of a source of anti-mouse BMP2 (bone morphogenic protein) appropriate to use on FFPE or frozen mouse tissue? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linke_Noelle <@t> Allergan.com Tue Jul 6 12:04:02 2004 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] mouse on mouse Message-ID: Hi all, What is everyone using as far as mouse on mouse IHC? Are you happy with it? Thank you! Noelle Linke, BS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From laurie.colbert <@t> huntingtonhospital.com Tue Jul 6 12:07:36 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] knives and scissors with disposable blades? Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0CDF@EXCHANGE1.huntingtonhospital.com> Hazel, We order the scissors and the replaceable/disposable blades from Cardinal/ Allegiance. The part # for the scissors is D2891-14 and the part # for the blades is D2891-16 (5 prs/cs). You can order blades with either sharp/sharp tips or blunt/sharp tips. I have a 2000 Allegiance catalog and they're on page 433. There are actually some knives on page 432. We order our disposable knives from American Master Tech, part #BL5000. Laurie Colbert -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, July 06, 2004 9:29 AM To: histonet@pathology.swmed.edu Subject: [Histonet] knives and scissors with disposable blades? We have a new pathologist fresh out of school. She tells me the place she was a resident (somewhere in Texas) had long knives and scissors with disposable blades. Can any one help me out with this. I have searched the internet but did not come up with anything. Thanks. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Tue Jul 6 12:18:47 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] enhanced DAB from Pierce Message-ID: Did anyone ever say they have used this new product, I missed it if they did? Can someone tell me how to contact Pierce for this product? Thank you, Patsy Ruegg From Allison_Scott <@t> hchd.tmc.edu Tue Jul 6 12:36:25 2004 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Knives and Scissors with disposable blades Message-ID: These items can be found in the Allegiance (Cardinal) catalog 1-800-964-5227. We use the knives and the scissors. You can get the knife handles and blades in different sizes. They are called, Accu-edge trimming knife handles and disposable blades. They are on page 432 in the catalog. The scissors are called Accu-edge Replaceable blade scissors and blades. They are on page 433. I hope that this helps. Allison Scott HT (ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Allison_Scott <@t> hchd.tmc.edu Tue Jul 6 12:50:03 2004 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:23:41 2005 Subject: FW: [Histonet] Knives and Scissors with disposable blades Message-ID: -----Original Message----- From: Scott, Allison D Sent: Tuesday, July 06, 2004 12:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Knives and Scissors with disposable blades These items can be found in the Allegiance (Cardinal) catalog 1-800-964-5227. We use the knives and the scissors. You can get the knife handles and blades in different sizes. They are called, Accu-edge trimming knife handles and disposable blades. They are on page 432 in the catalog. The scissors are called Accu-edge Replaceable blade scissors and blades. They are on page 433. I hope that this helps. Allison Scott HT (ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Allison_Scott <@t> hchd.tmc.edu Tue Jul 6 12:50:56 2004 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:23:41 2005 Subject: FW: [Histonet] Knives and Scissors with disposable blades Message-ID: -----Original Message----- From: Scott, Allison D Sent: Tuesday, July 06, 2004 12:36 PM To: 'histonet@lists.utsouthwestern.edu Subject: [Histonet] Knives and Scissors with disposable blades These items can be found in the Allegiance (Cardinal) catalog 1-800-964-5227. We use the knives and the scissors. You can get the knife handles and blades in different sizes. They are called, Accu-edge trimming knife handles and disposable blades. They are on page 432 in the catalog. The scissors are called Accu-edge Replaceable blade scissors and blades. They are on page 433. I hope that this helps. Allison Scott HT (ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From lu_ze <@t> sbcglobal.net Tue Jul 6 15:56:11 2004 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Manual for Fisher histomatic 266 tissue processor, help References: <200407061701.i66H1h9A212882@vmh.prodigy.net> Message-ID: <001e01c4639b$ab4ce300$0f1515ce@optimum2> Hello, Histonet friends, We bought a used Fisher Histomatic 266 MP Tissue Processor recently. There is no manual with it. We called Fisher and they don't have it as it is too old. Anybody here have the manual for this model? Can we have a copy? Actually we already figured out most of functions. Only one bottle with kind of "float" inside and two connection tube on the top. I guess it is for vaccum operation. Can not figure out how to connect it. Also, there are two plastic tubes on the backe othe machine. Are they for exhaust? Thank you for your help. We appreciate your help. Ze Lu, Ph.D. Optimum Therapeutics, LLC From Bill.Logan <@t> CVIHR.BC.CA Tue Jul 6 13:03:31 2004 From: Bill.Logan <@t> CVIHR.BC.CA (Logan, Bill) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] used equipment Message-ID: <5BC0EC4F2A8BD4119DA800508B952F9346EF1A@cvihr61.cvihr.bc.ca> We have a pathologist visiting from Laos and would like some information as regards suppliers of refurbished pathology processors etc., which could possibly be purchased and sent to the Far East. Thanks. From Allison_Scott <@t> hchd.tmc.edu Tue Jul 6 13:05:27 2004 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:23:41 2005 Subject: FW: [Histonet] Knives and Scissors with disposable blades Message-ID: -----Original Message----- From: Scott, Allison D Sent: Tuesday, July 06, 2004 12:51 PM To: 'histonet@lists.utsouthwestern.edu' Subject: FW: [Histonet] Knives and Scissors with disposable blades -----Original Message----- From: Scott, Allison D Sent: Tuesday, July 06, 2004 12:36 PM To: 'histonet@lists.utsouthwestern.edu Subject: [Histonet] Knives and Scissors with disposable blades These items can be found in the Allegiance (Cardinal) catalog 1-800-964-5227. We use the knives and the scissors. You can get the knife handles and blades in different sizes. They are called, Accu-edge trimming knife handles and disposable blades. They are on page 432 in the catalog. The scissors are called Accu-edge Replaceable blade scissors and blades. They are on page 433. I hope that this helps. Allison Scott HT (ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From gu.lang <@t> gmx.at Tue Jul 6 13:35:02 2004 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] fixing and processing testicular biops. Message-ID: <009201c46387$f3b7f2b0$eeeea8c0@SERVER> Dear histonetters Our usual treatment of testicular biopsies is: fixing a few hours in bouin's solution, then putting them together with all other biopsies in the VIP. processing begins with NFB-70-80-clearing-paraffin, over night. 1. question: Does this treatment make sense at all? Is it estimated, that the result would be the same with or without bouin? 2. please let me know about a proper processing-protocol for testicular biopsies. (needle-biopsies, VIP) 3. Do you know any good substitute for bouin, that does'nt effect Immunhisto for this purpose? many thanks Gudrun Lang Akh Linz, Austria From lu_ze <@t> sbcglobal.net Tue Jul 6 16:45:43 2004 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Manual for Fisher histomatic 266 tissue processor, help Message-ID: <003501c463a2$96606cd0$0f1515ce@optimum2> Hello, Histonet friends, We bought a used Fisher Histomatic 266 MP Tissue Processor recently. There is no manual with it. We called Fisher and they don't have it as it is too old. Anybody here have the manual for this model? Can we have a copy? Actually we already figured out most of functions. Only one bottle with kind of "float" inside and two connection tube on the top. I guess it is for vaccum operation. Can not figure out how to connect it. Also, there are two plastic tubes on the backe othe machine. Are they for exhaust? Thank you for your help. We appreciate your help. Ze Lu, Ph.D. Optimum Therapeutics, LLC From gu.lang <@t> gmx.at Tue Jul 6 15:28:00 2004 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] fixing and processing testicular biops. References: <0556BE8AC5551E4E8AF6BB9E42509BA206EC72@usca0082k08.labvision.apogent.com> Message-ID: <00ee01c46397$bb43f0e0$eeeea8c0@SERVER> Dear Tim, I've read controvers texts. On one side John Kiernan writes, that is does not make sense to put bouin-fixed tissue in NFB. (because it solutes the picrates?) On the other side I read, that the tissue should be washed in water or Lithiumcarbonatsolution. My problem is, if we should create a proper protocol for our biospies or if the old one is good enough. Gudrun Lang ----- Original Message ----- From: "Morken, Tim - Labvision" To: "'Gudrun Lang'" Sent: Tuesday, July 06, 2004 8:50 PM Subject: RE: [Histonet] fixing and processing testicular biops. > Gudrun, Bouin's is the preferred fixative for testis. And Bouin's is > generally an excellent fixative for immunohistochemistry. Normally, bouin's > - fixed tissue should be rinsed in water for 30 minutes after fixation and > before further processing > > Tim Morken > Lab Vision - Neomarkers > www.labvision.com > > Free webhosting for US State Histotechnology Societies: > http://www.labvisioncorp.com/demowebsite/index.cfm > > -----Original Message----- > From: Gudrun Lang [mailto:gu.lang@gmx.at] > Sent: Tuesday, July 06, 2004 11:35 AM > To: Histonetliste > Subject: [Histonet] fixing and processing testicular biops. > > > Dear histonetters > Our usual treatment of testicular biopsies is: > fixing a few hours in bouin's solution, then putting them together with all > other biopsies in the VIP. > processing begins with NFB-70-80-clearing-paraffin, over night. > > 1. question: > Does this treatment make sense at all? Is it estimated, that the result > would be the same with or without bouin? 2. please let me know about a > proper processing-protocol for testicular biopsies. (needle-biopsies, VIP) > 3. Do you know any good substitute for bouin, that does'nt effect Immunhisto > for this purpose? > > many thanks > Gudrun Lang > Akh Linz, Austria _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gu.lang <@t> gmx.at Tue Jul 6 11:52:33 2004 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] fixing and processing - testicular biopsy Message-ID: <001e01c46379$a23743e0$eeeea8c0@SERVER> Dear histonetters Our usual treatment of testicular biopsies is: fixing a few hours in bouin's solution, then putting them together with all other biopsies in the VIP. processing begins with NFB-70-80-clearing-paraffin, over night. 1. question: Does this treatment make sense at all? Is it estimated, that the result would be the same with or without bouin? 2. please let me know about a proper processing-protocol for testicular biopsies. (needle-biopsies, VIP) 3. Do you know any good substitute for bouin, that does'nt effect Immunhisto for this purpose? many thanks Gudrun Lang Akh Linz, Austria From minkk <@t> zgi.com Tue Jul 6 12:10:49 2004 From: minkk <@t> zgi.com (KMIN (Kathy Mink)) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] mouse on mouse Message-ID: We use the DAKO ARK kit and are very happy with it. Kathy Mink ZymoGenetics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Linke_Noelle Sent: Tuesday, July 06, 2004 10:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse on mouse Hi all, What is everyone using as far as mouse on mouse IHC? Are you happy with it? Thank you! Noelle Linke, BS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Tue Jul 6 17:21:57 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Back to manual mode Message-ID: I am seeing some repeating messages, and will go back to manual mode until I figure out where they are coming from. Thanks, Herb From LuckG <@t> empirehealth.org Tue Jul 6 17:39:16 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] (no subject) Message-ID: Hello, We have an opening for a full time ASCP certified Cytotechnologist M-F, 07:30-16:00. We do about 15,000 gyn cases (predominantly Surepath liquid based) and 1,000 non-gyn cytology cases. We are a 200+ bed tertiary care community Medical Center. The cytology and histology sections are combined into the Anatomic Pathology division. When full staffed we have two cytotechs, two A.P. lab assistants, four histotechs plus me. Anyone interested (or please share with anyone you feel may be) please contact me for further information. This position will be open in two weeks and we are anxious to fill it. Thanks for your time, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org From gcallis <@t> montana.edu Tue Jul 6 17:53:46 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Veterinary, Industry and Research (VIR), NSH needs committee table volunteers for Toronto meeting Message-ID: <3.0.6.32.20040706165346.00bff1e8@gemini.msu.montana.edu> Dear All, On behalf of the VIR Chairman, the VIR committee needs volunteers to help "man" the committee table during the National Society for Histotechnology symposium/convention in Toronto from September 18 to September 22nd. In general, this means someone must sit at the committee table during breaks (morning, afternoon and lunch breaks) on Sat, Sunday, Monday, Tuesday, probably only morning on Wednesday unless the tables are being taken down at that time. If VIR/NSH members are planning on attending the meeting, let us know if you can help during one or two of the breaks in a day. It doesn't entail a great deal of work as handouts, surveys, and general information etc will be there in advance. Please contact the VIR chairperson, Diane Sterchi (sterchi_diane_l@lilly.com) to let her know what times you are available to help. Diane will schedule you for a time slot and fill you in on minor duties. Thank you in advance Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Tue Jul 6 18:05:00 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] RE: mouse on mouse In-Reply-To: Message-ID: <3.0.6.32.20040706170500.00bff1e8@gemini.msu.montana.edu> We have had good luck with the new Chemicon mouse on mouse kit. It was very clean. Sometimes an antibody doesn't like biotinylation (ARK kit) and you have to try another kit. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From laurie.colbert <@t> huntingtonhospital.com Tue Jul 6 18:13:27 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] CAP question - cryostat decontamination Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BF99@EXCHANGE1.huntingtonhospital.com> I am asking a question for a lab manager from another lab, and she did not have the exact question in front of her, so bear with me. On the CAP survey, there is a question about decontaminating the cryostats for the AFB organism. Supposedly, this is a new quesion. Does anyone have the exact question and/or the correct procedure? Is the normal decontamination procedure (95-100% alcohol) efficient? Laurie Colbert Huntington Hospital Pasadena, CA From conniegrubaugh <@t> hotmail.com Tue Jul 6 18:20:46 2004 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Nevada Society Of Histotechnology Message-ID: The Nevada Society of Histotechnology will be having a weekend seminar Ocober 29th and 30th 2004, in Las Vegas. If you would like further information please email me with your postal address and I will send out the information or you can call me at 702-396-4079. Connie G. _________________________________________________________________ [1]Get tips for maintaining your PC, notebook accessories and reviews in Technology 101. References 1. http://g.msn.com/8HMBENUS/2749??PS=47575 From emry <@t> u.washington.edu Tue Jul 6 18:57:33 2004 From: emry <@t> u.washington.edu (P. Emry) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] paraformaldehyde Message-ID: I have never made this fix. The boss wants 4 liters. Please tell me how this is done. The max I find is 100ml. Thanks, Trisha U of Washington Seattle From ernestinemiddleton <@t> yahoo.ca Tue Jul 6 19:47:25 2004 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Position opening Message-ID: <20040707004725.67806.qmail@web51507.mail.yahoo.com> Hi, The Department of Pathology of Montefiore Med. Center, the University Hospital of Albert Einstein College of Medicine is seeking a full time Assistant to Histology Supervisor. The laboratory performs all routine histology as well as immunohistochemistry and special stain procedures, including direct immunofluorescence for skin and renal biopsies and histochemical studies for muscle biopsies. Primary responsibilities would include direct supervision of the special studies segment of the laboratory. The position requires a BS degree, at least 3 years experience in immunohistochemistry, as well as supervisory and histology experience. Knowledge of automation in immunohistochemistry and histology preferred. Ernestine Middleton, BS,MPA, HT/HTL Montefiore Med. Center Histology Laboratory 718-920-4157 718-547-1920 Fax --------------------------------- Post your free ad now! Yahoo! Canada Personals From asmith <@t> mail.barry.edu Tue Jul 6 20:00:48 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] nissl stain Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3B63@exchsrv01.barrynet.barry.edu> A good Nissl stain stains only RNA, but some "Nissl stains" also stain DNA. It's easy to tell the Nissl bodies from the nuclei, because they are smaller and irregular. Since Nissl bodies have high concentrations of RNA, they stain darkly. Nissl bodies are characteristic of neurons with long axons. Since neurons with short axons and glial cells have low concentrations of RNA, they stain weakly, if at all. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mgorin@umich.edu Sent: Tuesday, July 06, 2004 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nissl stain sry guys i am new at this. but can somone tell me if nissl, when doen to brain tissue, will only stain neurons? will it stain glial cells? also, how does nissl work. wat bodies does it stain. thanks. -mike- UMICH- Ann Arbor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From emry <@t> u.washington.edu Tue Jul 6 20:02:35 2004 From: emry <@t> u.washington.edu (P. Emry) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] archives Message-ID: Hi, How do I get into the archives? Thanks, Trisha From MinHan.Tan <@t> vai.org Tue Jul 6 20:28:06 2004 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Staining of nucleus - real? Message-ID: <74D0F0AB07F2E647A02D839ED79520F9B488F5@VAIEXCH02.vai.org> Good evening, I'd like to ask a question - we have recently purchased a polyclonal cadherin antibody from Santa Cruz that does stain the inter-cellular compartment, but unexpectedly, also stains the nucleus in some cells in kidney tissue. (no other tissue reflects this kind of staining), as far as we can assess. One pathologist thought it was a 'background' nuclear staining; another felt it was likely to be a true stain. Although we are using the ABC system, it is not a biotin-derived staining, since our controls omitting primary antibody are negative and the staining is not cytoplasmic. How can we evaluate whether this staining is due to the antibody binding to the relevant antigen, or whether this reflects cross-reactivity, or whether this is 'background nuclear staining'? Any advice would be very much appreciated! Regards, Min-han This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. From JAnorthernexp <@t> cs.com Tue Jul 6 21:18:16 2004 From: JAnorthernexp <@t> cs.com (JAnorthernexp@cs.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Re: Ventana Systems Message-ID: <46.528969a2.2e1cb768@cs.com> Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA From rockbeki <@t> ufl.edu Tue Jul 6 22:02:55 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] weird brownish stuff in AEC slides Message-ID: <115557129.1089169375651.JavaMail.osg@spnode33> Hey all! Here's an update on the brownish stains on my aec slides. (Btw, I sadly don't have pictures of what I'm talking about because our new microscope with camera has not yet arrived.)I tried to dilute my enzyme conjugate by 25% in the hopes it would avoid the metachromasia problem, but the brown stains are still there (although lighter) and worse, the AEC is barely visible. I think those of you who suggested it could be blood in the tissue might have a point, but my professor is not overly fond of using picric acid so if any of y'all have any other ideas, that would be great. Also, if any of you ever do both DAB and AEC in your labs, is it possible that one set of DAB slides could get DAB onto AEC slides if the some of the reagents (like PBS) are shared? (Another tech was working on his slides at the same time that i was doing my run.) Thanks! Rebekah Smith On Thu Jul 01 11:11:30 EDT 2004, Bryan Hewlett wrote: > Rebekah, > > I believe that what you are describing is classic so-called > metachromasia of > the AEC reaction product. > Metachromasia of the AEC reaction product is seen as a > progressive change in > colour from the normal rose-red to red-brown, followed by brown, > yellow-brown, then brownish-green to yellowish-green. > > Metachromasia occurs in areas of high enzyme density > (concentration). Enzyme > density is influenced by both the local antigen density and the > amount of > attached primary antibody that is the target for the detection > reagents. > Therefore, the concentration of the primary antibody directly > implies the > local enzyme concentration, on the basis of a given antigenic > density in the > section. > > > > At low concentrations of peroxidase enzyme, it has been proposed1 > that the > reaction ends in the formation of a stable polymeric complex of > red colour > (Wursters Red), since the decay to a precipitate occurs more > rapidly than > further enzymatic oxidation. > > High concentrations of peroxidase accelerate the oxido-reductive > process to > the extent that there is insufficient time for the stable red > polymer to > form and precipitate. Instead, further rapid oxidation results in > the > formation and precipitation of a yellowish-green quinone di-imine > product. > Intermediate colours are a result of both reactions. > > > > Metachromasia in high antigen density areas is more prone to > occur at > temperatures above 24?C. > > Slight cooling of the AEC working solution will often correct > this. > > If metachromasia in high antigen density areas persists despite > lowering the > temperature, > > or if some antibodies consistently demonstrate the phenomenon, > reduction of > the detection > > threshold by further dilution of the primary antibody is > necessary. > > > > We often see this metachromatic effect during the optimization > process for > new antibodies. > > Re-titration of the primary antibody to a lower concentration > always > restores the rose-red colour. > > > > Bryan > > > > > > Reference. > > 1 Koretz K, Leman J, Brandt I, M?ller P: Metachromasia of > 3-amino-9-ethylcarbazole (AEC) and its prevention in > immunoperoxidase > techniques. Histochemistry 1987; 86: 471-478. > > > > > > > > > > ----- Original Message ----- > From: "SMITH,REBEKAH FELICIA" > To: > Sent: Thursday, July 01, 2004 9:52 AM > Subject: [Histonet] weird brownish stuff in AEC slides > > >> I have yet another question, guys. I've been asking a lot of >> questions about blocking endogenous peroxidase,but now I have a >> new situation. I'm still using the ABC peroxidase method staining >> sheep placenta (formalin fixed, paraffin embedded) for eNOS and >> using AEC as a chromogen and gill's hematoxylin (blued with tap >> water and water with 2 or 3 drops of ammonia) as a counterstain. >> (btw, still blocking peroxidase with 30% peroxide in methanol, >> since my professors are away and haven't been able to order >> anything better) I have the lovely red and blue contrast in most >> parts of my slides, but I also am getting areas of brownish-black >> staining (kinda DAB colored, although I'm not using any DAB). I'm >> not exactly sure where the brown comes from this and whether its >> a >> problem with my AEC, my hematoxylin or something else. Anyone >> have >> any ideas? Thanks in advance, y'all have already been really >> helpful. >> >> -- >> SMITH,REBEKAH FELICIA >> "You are a child of the universe, no less than the trees and the >> stars >> You have a right to be here and whether or not it is clear to >> you, >> no doubt the universe is unfolding as it should. Therefore be at >> peace with G-d, whatever you conceive Him to be. And whatever >> your >> labors and aspirations,in the noisy confusion of life, keep peace >> in your soul.-Max Ehrmann,"Desiderata" >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From c.m.vanderloos <@t> amc.uva.nl Wed Jul 7 01:55:22 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] RE:Recipe for Tris-EDTA, pH 9.0 for Antigen Retreival Message-ID: <78ad8478a9cb.78a9cb78ad84@amc.uva.nl> Dear Robert, Our recipe looks like this: 10 mM Tris / 1 mM EDTA buffer, pH 9.0 Dissolve 1.2 gram Tris in ? 950 ml distilled water. Adjust pH to ? 9.5 with 2-4 drops of 1M HCl and add 0.37 gram EDTA (di-sodium salt). Adjust pH to 9.0 with 1M HCl, and fill to 1 ltr with distilled water. Store at 4?C. Hope this works for you. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From "Lott, Robert" Date Tue, 6 Jul 2004 11:48:47 -0500 To Subject [Histonet] Recipe for Tris-EDTA, pH 9.0 for Antigen Retreival Anyone got a recipe for the solution above? How do you dissolve the EDTA in the solution? Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com From pengbw <@t> sjtu.edu.cn Wed Jul 7 03:15:51 2004 From: pengbw <@t> sjtu.edu.cn (Baowei Peng) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Ab to mouse macrophages Message-ID: <20040707081551.56AE41114F9A@sjtu.edu.cn> Hi, all histoneters, Can anyone recommand a mAb to identify mouse macrophages in flow cytometry assay? Baowei Peng Shanghai Jiaotong University Shanghai,20030 China From JAnorthernexp <@t> cs.com Wed Jul 7 03:35:02 2004 From: JAnorthernexp <@t> cs.com (JAnorthernexp@cs.com) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] (no subject) Message-ID: <5a.2d955f6e.2e1d0fb6@cs.com> Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA From c.m.vanderloos <@t> amc.uva.nl Wed Jul 7 05:22:48 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] RE: Staining of nucleus - real? Message-ID: <6331f66328e4.6328e46331f6@amc.uva.nl> Dear Min-han, The problem as you described whether or not the staining of an antibody is real is a bit like the chicken-versus-egg problem: difficult to say something sensible about it. I would like to share some thoughts from the practical point of view: 1. As you already said, consider your negative controls first. Tissue pretreatments, fixation, detection systems and chromogens all may have their influence on background staining. Of course this should be excluded first. 2. Try to find a "trustful" positive control. Tissues mentioned in literature, cultured cells, rare tumors, etc. may be very helpful. 3. Consider the point of leaking away of antigens during fixation, and perhaps precipitating elsewhere at the tissue section. Especially when working with cyro's one should be careful with the interpretation of IHC using anti-cytokines, chemokines. 4. The IgG concentration of the diluted antibody. Once above 2-5 ug/ml you should be suspicious about your staining result. Non-specific staining above 5 ug/ml easily occurs (although there are also some positive examples in this respect). 5. When working with FFPE sections test different tissue pretreatment procedures (nothing - pepsin or pronase - HIER/citrate6.0 - HIER/EDTA9.0 - "exotic" pretreatments). Again this may yield different staining patterns, but there should be at least some similar staining features. 6. Compare the staining patterns of different antibodies to the same antigen/epitope. Certainly not the most economic way, but sometimes very informative (not to say "revealing"). 7. Finally, you have go from the testing phase to the target tissue. At this point you have to select an antibody, fixation, tissue pretreatment, detection system, etc. that all together yield a staining result you feel comfortable with. This remains a scarry moment. I do agree with that! Personally I think that the IHC evaluation of all those new research-type of antibodies on the marked, is perhaps one of the biggest problems we have to struggle with in the next few years. I hope my thoughts at least gives you some moral support. Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From "Tan, MinHan" Date Tue, 6 Jul 2004 21:28:06 -0400 To Subject [Histonet] Staining of nucleus - real? Good evening, I'd like to ask a question - we have recently purchased a polyclonal cadherin antibody from Santa Cruz that does stain the inter-cellular compartment, but unexpectedly, also stains the nucleus in some cells in kidney tissue. (no other tissue reflects this kind of staining), as far as we can assess. One pathologist thought it was a 'background' nuclear staining; another felt it was likely to be a true stain. Although we are using the ABC system, it is not a biotin-derived staining, since our controls omitting primary antibody are negative and the staining is not cytoplasmic. How can we evaluate whether this staining is due to the antibody binding to the relevant antigen, or whether this reflects cross-reactivity, or whether this is 'background nuclear staining'? Any advice would be very much appreciated! Regards, Min-han From vbaker60 <@t> yahoo.com Wed Jul 7 07:11:04 2004 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] archives In-Reply-To: Message-ID: <20040707121104.26208.qmail@web50103.mail.yahoo.com> http://www.histosearch.com/histonet.html I have it saved to my favorites, as I use it very, very often. Vikki Baker Institute for Cancer Prevention Valhalla, NY 10595 --- "P. Emry" wrote: > Hi, > How do I get into the archives? > > Thanks, > > Trisha > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From lu_ze <@t> sbcglobal.net Wed Jul 7 10:38:22 2004 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Manual for Fisher histomatic 266 tissue processor, help Message-ID: <001801c46438$6fad96d0$0f1515ce@optimum2> Hello, Histonet friends, We bought a used Fisher Histomatic 266 MP Tissue Processor recently. There is no manual with it. We called Fisher and they don't have it as it is too old. Anybody here have the manual for this model? Can we have a copy? Actually we already figured out most of functions. Only one bottle with kind of "float" inside and two connection tube on the top. I guess it is for vaccum operation. Can not figure out how to connect it. Also, there are two plastic tubes on the backe othe machine. Are they for exhaust? Thank you for your help. We appreciate your help. Ze Lu, Ph.D. Optimum Therapeutics, LLC From juan.gutierrez <@t> christushealth.org Wed Jul 7 07:39:06 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:41 2005 Subject: [Histonet] Re: Ventana Systems Message-ID: Hello Annette! We have the original Benchmarks and we do all our depar. and retrieval on it. As for slides, yes we use coated(charged) slides. We have tried different vendors and have gotten good and bad results with ALL of them. I think it's a slide manufacturer QC problem. We dry them in a Desert Chamber at 70-75C for 15 min before loading on the Benchmark. We use to decloak and pretreat, but with a little trial and error managed to move all of our pretreatments to the machines. Good luck! Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: JAnorthernexp@cs.com [mailto:JAnorthernexp@cs.com] Sent: Tuesday, July 06, 2004 9:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ventana Systems Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Wed Jul 7 08:07:05 2004 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene recycling Message-ID: Good Morning All, Our hospital is demoing a xylene recycler for the next few weeks. Of course, this was done without the lab's knowledge, but that is another story for another time. My question is how pure is the recycled xylene? Are you able to use the xylene on the processor as if it were new? I assume that you can use it for staining purposes, but I'm a bit wary about using it for processing tissue. The recycler that is here now is from B/R instruments and apparently another one from CBG Biotech will be here at the end of the month. Any assistance would be greatly appreciated. Thanks........ Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From SBarnes <@t> elch.org Wed Jul 7 08:10:21 2004 From: SBarnes <@t> elch.org (Sue Barnes) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene recycling Message-ID: We use the CBG recycler in our hospital. The recovered xylene was sent out for gas spectrophotometry and was verified as 99% pure. We use it in the processor as well as the stainer and have had no problems. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bell, Lynne Sent: Wednesday, July 07, 2004 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene recycling Good Morning All, Our hospital is demoing a xylene recycler for the next few weeks. Of course, this was done without the lab's knowledge, but that is another story for another time. My question is how pure is the recycled xylene? Are you able to use the xylene on the processor as if it were new? I assume that you can use it for staining purposes, but I'm a bit wary about using it for processing tissue. The recycler that is here now is from B/R instruments and apparently another one from CBG Biotech will be here at the end of the month. Any assistance would be greatly appreciated. Thanks........ Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Jul 7 08:23:56 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene recycling Message-ID: Recycled xylene is actually purer than new. We have found that this can cause GI biopsies to be crisper than when processed with new xylene, so we don't use it on our biopsy processor. Also, with some coverslippers, new xylene is a must. Other than a bad odor that can be remedied with acetic acid, I have had no problems with recycled xylene in the 20 yrs (yikes!) I've used it - with either of these brands of recyclers. Good luck! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bell, Lynne Sent: Wednesday, July 07, 2004 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene recycling Good Morning All, Our hospital is demoing a xylene recycler for the next few weeks. Of course, this was done without the lab's knowledge, but that is another story for another time. My question is how pure is the recycled xylene? Are you able to use the xylene on the processor as if it were new? I assume that you can use it for staining purposes, but I'm a bit wary about using it for processing tissue. The recycler that is here now is from B/R instruments and apparently another one from CBG Biotech will be here at the end of the month. Any assistance would be greatly appreciated. Thanks........ Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From pmarcum <@t> polysciences.com Wed Jul 7 08:42:08 2004 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene recycling In-Reply-To: Message-ID: <001c01c46428$329bb070$4000a8c0@PMARCUM2K> Hi, Joyce is right the recycled xylene tends to be purer and work better for some things and to well for others unless you adjust the processing schedule for it. Then you have to watch when you change back to the fresh from the bottle stuff. If you only have one processor just be careful as one friend of mine did years ago just run the freshly opened xylene through the recycler so it is always the purer form of xylenes. Xylenes that we normally use have a number of contaminates in them and we couldn't afford pure xylene in most cases. Thanks, Pam Marcum? > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, > Joyce > Sent: Wednesday, July 07, 2004 9:24 AM > To: Bell, Lynne; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Xylene recycling > > > Recycled xylene is actually purer than new. We have found that > this can cause GI biopsies to be crisper than when processed with > new xylene, so we don't use it on our biopsy processor. Also, > with some coverslippers, new xylene is a must. Other than a bad > odor that can be remedied with acetic acid, I have had no > problems with recycled xylene in the 20 yrs (yikes!) I've used it > - with either of these brands of recyclers. > > Good luck! > > Joyce > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bell, > Lynne > Sent: Wednesday, July 07, 2004 9:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene recycling > > > Good Morning All, > > Our hospital is demoing a xylene recycler for the next few weeks. Of > course, this was done without the lab's knowledge, but that is another > story for another time. My question is how pure is the recycled xylene? > Are you able to use the xylene on the processor as if it were new? I > assume that you can use it for staining purposes, but I'm a bit wary > about using it for processing tissue. The recycler that is here now is > from B/R instruments and apparently another one from CBG Biotech will be > here at the end of the month. > > > > Any assistance would be greatly appreciated. Thanks........ > > > > Lynne A. Bell, HT (ASCP) > > Central Vermont Hospital > > P. O. Box 547 > > Barre, VT 05641 > > 802-371-4122 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this > message may be privileged and is confidential information > intended for the use of the addressee listed above. If you are > neither the intended recipient nor the employee or agent > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us > immediately by replying to the message and deleting it from your computer. > Thank you. Saint Josephs Health System, Inc. > > From HornHV <@t> archildrens.org Wed Jul 7 09:15:46 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] HT's must be registered? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6322B8DD@EMAIL.archildrens.org> I have been asked this question now twice. They are wanting to know if HT's have to be registered. Is it required by law, CAP, or any other authority? I know we would love for all techs to be registered but I didn't think it was mandatory? Thanks as always for your help. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From mgorin <@t> umich.edu Wed Jul 7 09:56:50 2004 From: mgorin <@t> umich.edu (mgorin@umich.edu) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] nissl strain Message-ID: <1089212210.40ec0f32bca35@mail.umich.edu> i did a nissl stain w. cresyl violet. after my last xylene step i have been letting the slides dry off, and i visulize them (w. out a cover slip, these slides will be used for LCM), and the slides look great. if after about 5 min of drying i look at them, all of a sudden this darkness overcomesthe slide and the tissue looks black in color. i can actauly view the blackness overcome the slide. then if i redip it in xylene, the blackness goes awya and the slides look awsome again. i find if i cover slip the slides prior to teh blackness, they preserve in the good looking state. why is this happening? how can i stop it from happening w/ our a cover slip? wat is going on here? someone in my lab suggested oxidation, but i am not quite sure wat thta is all about. -mike- ann arbor From gcallis <@t> montana.edu Wed Jul 7 10:08:25 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: histonet enquiry In-Reply-To: <6.0.0.22.2.20040707121536.01ba2210@mail.staff.unimelb.edu. au> Message-ID: <3.0.6.32.20040707090825.00c14ae8@gemini.msu.montana.edu> The full book title is Antiogen Retrieval Techniques, Immunohistochemistry and Molecular Morphology by Shi, S-R, Gu J, and Taylor CR. The book was published by Eaton, and the authors are key to which book to get, done by the experts. At 12:16 PM 7/7/2004 +1000, you wrote: > Gayle, > >" " by Shi, Gu and Taylor is THE book to own on Antigen unmasking, >retrieval etc. I have been trying to order it on the Biotechniques website >and it seems that there are 2 volumes of the book. > One is : > Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology > and the other is > The Antigen Retrieval Technique: Applications in Immunohistochemistry > > Looking at their chapter, they seem to have the same contents but for some >reason there are 2 books. I had a look at the publication dates for them >and they are a month apart. Which of these is the book that you've got and >recommend? > Thanks a bunch... > Regards, Melanie > > > Melanie de Silva > Victorian Breast Cancer Research Consortium > Level 6, Department of Pathology > Medical Building > University of Melbourne > Ph: 8344 5883 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From dmccaig <@t> ckha.on.ca Wed Jul 7 10:13:47 2004 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Tissue Tek Coverslipper Model 4764 Message-ID: <3E5A3F039F0BD8118B4700C00D0020240431FD@CKHA9> I was wondering if anyone has experienced the same issues we are having with this coverslipper. It started last week by skipping the occasional slide and double coverslipping the one behind it. Then it started so apply the ribbon about 1 cm up on the slide instead of near the lower edge. It would also cut at various lengths. No, when the slides pass, the ribbon is cut but it accumulates by the rollers and the slides pass through uncoverslipped. The roll has been changed and it was allowed 24 hrs to climatize prior to usage. It has been cleaned and there is apparent debris on the chains. Any ideas? Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (6604) From Sue.Kapoor <@t> uhsi.org Wed Jul 7 10:22:13 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: Ventana Systems Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F560C@khmcexch.uhsi.org> Does anyone use a microwave to dry slides? Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 -----Original Message----- From: JAnorthernexp@cs.com [mailto:JAnorthernexp@cs.com] Sent: Tuesday, July 06, 2004 9:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ventana Systems Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> unc.edu Wed Jul 7 10:12:18 2004 From: robert_schoonhoven <@t> unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] nissl strain In-Reply-To: <1089212210.40ec0f32bca35@mail.umich.edu> References: <1089212210.40ec0f32bca35@mail.umich.edu> Message-ID: <40EC12D2.3080406@unc.edu> Mike, Short answer: What is "happening" is that the tissue on the slide is drying out as the xylene evaporates. The liquid xylene acts similar to a coverslip. Robert Schoonhoven mgorin@umich.edu wrote: >i did a nissl stain w. cresyl violet. after my last xylene step i have been >letting the slides dry off, and i visulize them (w. out a cover slip, these >slides will be used for LCM), and the slides look great. > >if after about 5 min of drying i look at them, all of a sudden this darkness >overcomesthe slide and the tissue looks black in color. i can actauly view the >blackness overcome the slide. > >then if i redip it in xylene, the blackness goes awya and the slides look awsome >again. i find if i cover slip the slides prior to teh blackness, they preserve >in the good looking state. why is this happening? how can i stop it from >happening w/ our a cover slip? wat is going on here? > >someone in my lab suggested oxidation, but i am not quite sure wat thta is all >about. > >-mike- >ann arbor > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From juan.gutierrez <@t> christushealth.org Wed Jul 7 10:38:18 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: Ventana Systems Message-ID: Only when absolutely necessary. Three minutes full power. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kapoor, Sue [mailto:Sue.Kapoor@uhsi.org] Sent: Wednesday, July 07, 2004 10:22 AM To: 'JAnorthernexp@cs.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ventana Systems Does anyone use a microwave to dry slides? Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 -----Original Message----- From: JAnorthernexp@cs.com [mailto:JAnorthernexp@cs.com] Sent: Tuesday, July 06, 2004 9:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ventana Systems Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Jul 7 10:38:18 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: Ventana Systems Message-ID: Only when absolutely necessary. Three minutes full power. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kapoor, Sue [mailto:Sue.Kapoor@uhsi.org] Sent: Wednesday, July 07, 2004 10:22 AM To: 'JAnorthernexp@cs.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ventana Systems Does anyone use a microwave to dry slides? Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 -----Original Message----- From: JAnorthernexp@cs.com [mailto:JAnorthernexp@cs.com] Sent: Tuesday, July 06, 2004 9:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ventana Systems Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> unc.edu Wed Jul 7 10:12:18 2004 From: robert_schoonhoven <@t> unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] nissl strain In-Reply-To: <1089212210.40ec0f32bca35@mail.umich.edu> References: <1089212210.40ec0f32bca35@mail.umich.edu> Message-ID: <40EC12D2.3080406@unc.edu> Mike, Short answer: What is "happening" is that the tissue on the slide is drying out as the xylene evaporates. The liquid xylene acts similar to a coverslip. Robert Schoonhoven mgorin@umich.edu wrote: >i did a nissl stain w. cresyl violet. after my last xylene step i have been >letting the slides dry off, and i visulize them (w. out a cover slip, these >slides will be used for LCM), and the slides look great. > >if after about 5 min of drying i look at them, all of a sudden this darkness >overcomesthe slide and the tissue looks black in color. i can actauly view the >blackness overcome the slide. > >then if i redip it in xylene, the blackness goes awya and the slides look awsome >again. i find if i cover slip the slides prior to teh blackness, they preserve >in the good looking state. why is this happening? how can i stop it from >happening w/ our a cover slip? wat is going on here? > >someone in my lab suggested oxidation, but i am not quite sure wat thta is all >about. > >-mike- >ann arbor > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Wed Jul 7 10:46:17 2004 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: Ventana Systems Message-ID: We do: 2" in a 900 watt oven. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Kapoor, Sue [mailto:Sue.Kapoor@uhsi.org] Sent: Wednesday, July 07, 2004 10:22 AM To: 'JAnorthernexp@cs.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ventana Systems Does anyone use a microwave to dry slides? Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 -----Original Message----- From: JAnorthernexp@cs.com [mailto:JAnorthernexp@cs.com] Sent: Tuesday, July 06, 2004 9:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ventana Systems Hi there, Does anyone out there use this system and what is your procedure for cutting (micron thickness), drying time and temperature for slides or do you air dry using charge slides. And do you use a decloaker for deparaffinizing and antigen retrieval. thanks, Annette Hart, HT (ASCP) Wyoming Valley Health Care System Wilkes-Barre, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rschoon <@t> email.unc.edu Wed Jul 7 10:56:34 2004 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] delete this e-mail - just a test to see if my add. is correct In-Reply-To: <40EC12D2.3080406@unc.edu> References: <1089212210.40ec0f32bca35@mail.umich.edu> <40EC12D2.3080406@unc.edu> Message-ID: <40EC1D32.8010707@email.unc.edu> From lleach <@t> uic.edu Wed Jul 7 11:00:30 2004 From: lleach <@t> uic.edu (Lu Leach) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] versican control Message-ID: <6.0.1.1.2.20040707105824.023ef9e0@tigger.uic.edu> Dear Netters, What are you folks out there using for controls with Versican staining? The data sheet suggested cartilage, but that seems strange to me. Any thoughts would be appreciated. Thank you, Lu Leach From nyilmaz <@t> mersin.edu.tr Wed Jul 7 09:25:54 2004 From: nyilmaz <@t> mersin.edu.tr (=?windows-1254?Q?Nejat_Y=FDlmaz?=) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Freeware for measuring area Message-ID: <003f01c4642e$508652b0$4e01a8c0@mersin.edu.tr> Dear Histonetters... We're trying to measure multi-selected areas from histological micrographs. But we couldn't find any suitable software. Does anybody know a freeware to measure for this purpose? Best regards... Dr. Necat Y?lmaz From ccdub <@t> earthlink.net Wed Jul 7 12:34:14 2004 From: ccdub <@t> earthlink.net (Cindy/Rick DuBois) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene Recycling Message-ID: <40131.1089221656864.JavaMail.root@fozzie.psp.pas.earthlink.net> ------------------------------ Message: 6 Date: Wed, 7 Jul 2004 09:07:05 -0400 From: "Bell, Lynne" Subject: [Histonet] Xylene recycling To: Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning All, Our hospital is demoing a xylene recycler for the next few weeks. Of course, this was done without the lab's knowledge, but that is another story for another time. My question is how pure is the recycled xylene? Are you able to use the xylene on the processor as if it were new? I assume that you can use it for staining purposes, but I'm a bit wary about using it for processing tissue. The recycler that is here now is from B/R instruments and apparently another one from CBG Biotech will be here at the end of the month. Any assistance would be greatly appreciated. Thanks........ Date: Wed, 7 Jul 2004 09:23:56 -0400 Central Vermont Hospital of mine did years ago just run the freshly opened xylene through the > problems with recycled xylene in the 20 yrs (yikes!) I've used it > - with either of these brands of recyclers. > > > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, AP, or any other authority? I know we would love for all techs to be registered but I didn't think it was mandatory? Thanks as always for your help. Date: Wed, 7 Jul 2004 10:56:50 -0400 >recommend? > Thanks a bunch... Message-ID: <3E5A3F039F0BD8118B4700C00D0020240431FD@CKHA9> Content-Type: text/plain Does anyone use a microwave to dry slides? Sue Kapoor, HT (ASCP) ------------------------------ >happening w/ our a cover slip? wat is going on here? > Subject: RE: [Histonet] Re: Ventana Systems To: "Kapoor, Sue" , , Kenosha, WI 262-653-5570 To: 'JAnorthernexp@cs.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ventana Systems Annette Hart, HT (ASCP) Wyoming Valley Health Care System We used the Labmaster recycler for about 15 years to recycle xylene and Alcohol. We found that we had to decrease the time in xylene on our processors when the small biopsies started looking "burned". We now use the CBG recycler and used the recycled xylene in all aspects of histology. We did have to add an extra alcohol in our stainline (we hand stain) to help clear the slides. We are extremely happy with the xylene product we are getting off of our recycler, and the cost savings has been phenomenal. Cindy DuBois, HT ASCP DELTA PATHOLOGY ASSOC. STOCKTON CA From lu_ze <@t> sbcglobal.net Wed Jul 7 15:37:30 2004 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Freeware for measuring area References: <200407071700.i67H0xsj021984@ylpvm05.prodigy.net> Message-ID: <00b601c46462$391e5bc0$0f1515ce@optimum2> Hello, Dr. Yylmaz, There is a software called "ImageJ" from NIH. The software is free distributed for research. And it can be downloaded from http://rsb.info.nih.gov/ij/ Ze Lu, Ph.D. Optimum Therapeutics, LLC > > Dear Histonetters... > > We're trying to measure multi-selected areas from histological micrographs. But we couldn't find any suitable software. Does anybody know a freeware to measure for this purpose? > > Best regards... > > Dr. Necat Y?lmaz > From jlinda <@t> ces.clemson.edu Wed Jul 7 13:53:15 2004 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: Freeware for measuring area Message-ID: <5.2.1.1.0.20040707145049.027bfe68@mailhost.ces.clemson.edu> Dr. Necat Y?lmaz, Try "Image J" at the National Institutes of Health: http://rsb.info.nih.gov/ij/ Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From haldana <@t> unimoron.edu.ar Wed Jul 7 14:21:07 2004 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] New web site of the Argentine Society of Histotechnology Message-ID: <001901c46457$90176080$a904a8c0@um.edu> The Argentine Society of histotechnology has the pleasure to communicate that a new web site of the Society has been created (http://www.ht.org.ar). It is devoted to spanish speaking people- We shall receive with pleasure the visit to the new web site. Thanks in advance to all Histonel mailing list. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html From Sandra.Etheridge <@t> gems8.gov.bc.ca Wed Jul 7 15:20:33 2004 From: Sandra.Etheridge <@t> gems8.gov.bc.ca (Etheridge, Sandra AGF:EX) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] ?Osmolarity Message-ID: <424FAC08A8CDFE4BADD6497AE75B5E2F0E0A86E0@atlas.gov.bc.ca> Hello, everyone, I was asked today by a fellow co-worker if I knew what osmolarity was and how to calculate it for a complex solution. She needs to find the osmolarity of a Phosphate Buffered Saline solution (made inhouse). She has the normal concentrations for all the components, but doesn't know where to go from there, and neither do I!!!! Any help or info is, as always, appreciated. Sandra Etheridge Animal Health Center BC Ministry of Agriculture, Food & Fisheries Abbotsford, BC From jnocito <@t> satx.rr.com Wed Jul 7 16:10:50 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] HT's must be registered? References: <9AE8AA9E1F644B4AA6C155FB6FD51C6322B8DD@EMAIL.archildrens.org> Message-ID: <003301c46466$e49a0f40$3b6ece44@yourxhtr8hvc4p> Hazel, no there is no requirement to be registered, but I do take a first look at those who are simply because they put forth the effort. A characteristic that I like when hiring people. I have some techs who are not registered, but am guiding them to see that it only benefits them. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Horn, Hazel V" To: Sent: Wednesday, July 07, 2004 9:15 AM Subject: [Histonet] HT's must be registered? I have been asked this question now twice. They are wanting to know if HT's have to be registered. Is it required by law, CAP, or any other authority? I know we would love for all techs to be registered but I didn't think it was mandatory? Thanks as always for your help. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -------------------------------------------------------------------------- ---- The information contained in this message may be privileged and confidential and protected from disclosure. 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Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- ---- From conniegrubaugh <@t> hotmail.com Wed Jul 7 16:19:49 2004 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Warthon Starry and Steiner Stains Message-ID: Could any one please direct me in finding out the origin of these two stains. I'am doing this for a research paper and I remember reading about it in an old staining book far too many years ago. Connie G. _________________________________________________________________ [1]MSN 9 Dial-up Internet Access helps fight spam and pop-ups now 2 months FREE! References 1. http://g.msn.com/8HMBENUS/2731??PS=47575 From jefthompson <@t> salud.unm.edu Wed Jul 7 16:33:16 2004 From: jefthompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene Substitutes Message-ID: Hello Histonetters, This may be an old, tired, and oft asked question, but what ARE the health risks of the d-limonene containing xylene alternatives? The butylated hydroxyanisole in Fisher's CitriSolv is a carcinogen 'suspect' and there are many MSDS mentions of the possible allergen status of d-limonene. Many also are the stories of headaches caused by the stuff. Biodegradable qualities notwithstanding, I personally don't think these products are as 'mother's milk' safe as some seem to think (I don't think they should be poured down the sink for instance). As a group who may use these products more and in a greater volume than any other, I'd like to hear what the histology community thinks about use, handling, and disposal regardless of what the various MSDS's have to say. Thanks, Jeff Jeffrey F. Thompson Associate Scientist University of New Mexico Health Sciences Center Department of Neurology BRF Room 136 915 Camino de Salud NE Albuquerque, NM 87131 USA jefthompson@salud.unm.edu Phone: (505) 272-8010 FAX: (505) 272-0607 From bills <@t> icpmr.wsahs.nsw.gov.au Wed Jul 7 16:51:31 2004 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene Substitutes In-Reply-To: Message-ID: <000001c4646c$905435e0$83a7080a@wsahs.nsw.gov.au> Jeff, Like you I have been sceptical about the so called "xylene substitutes". In my experience limonene based products have caused as many if not more symptons than xylene. We have only one area where limonene based material is used now and this is because one of the technicians showed adverse effects to xylene. What worries me is that more and more household cleaning products now contain 'lemon oil bases'. I think these companies have not researched the problems associated with this product. Any chemical must have some detrimental effect on the human body if not handled correctly. With formaldehyde now being classed as carcinogenic does that mean we have to stop using it? Laboratory workers have the lowest incidence of disease caused by exposure to formaldehyde because we handle it correctly and ensure correct ventilation. Surely the same applies to xylene use? Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jeffrey Thompson Sent: Thursday, 08 July 2004 7:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello Histonetters, This may be an old, tired, and oft asked question, but what ARE the health risks of the d-limonene containing xylene alternatives? The butylated hydroxyanisole in Fisher's CitriSolv is a carcinogen 'suspect' and there are many MSDS mentions of the possible allergen status of d-limonene. Many also are the stories of headaches caused by the stuff. Biodegradable qualities notwithstanding, I personally don't think these products are as 'mother's milk' safe as some seem to think (I don't think they should be poured down the sink for instance). As a group who may use these products more and in a greater volume than any other, I'd like to hear what the histology community thinks about use, handling, and disposal regardless of what the various MSDS's have to say. Thanks, Jeff Jeffrey F. Thompson Associate Scientist University of New Mexico Health Sciences Center Department of Neurology BRF Room 136 915 Camino de Salud NE Albuquerque, NM 87131 USA jefthompson@salud.unm.edu Phone: (505) 272-8010 FAX: (505) 272-0607 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. 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From asmith <@t> mail.barry.edu Wed Jul 7 16:57:31 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] ?Osmolarity Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3B67@exchsrv01.barrynet.barry.edu> Osmolarity = molarity X number of ions per mole In the case of phosphate buffered saline, there are 3 components, and you have to calculate the osmolarity of each component and add the osmolarities of the components to get the osmolarity of the solution. The osmolarity of monobasic sodium phosphate is 2 X its molarity because the hydrogens hardly dissociate. The osmolarity of dibasic sodium phosphate is 3 X its molarity because both sodiums fully dissociate, but the hydrogen doesn't. The osmolarity of the saline is 2 X molarity of sodium chloride Add all of the above to get the osmolarity of the solution. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Etheridge, Sandra AGF:EX Sent: Wednesday, July 07, 2004 4:21 PM To: (histonet@lists.utsouthwestern.edu) Subject: [Histonet] ?Osmolarity Hello, everyone, I was asked today by a fellow co-worker if I knew what osmolarity was and how to calculate it for a complex solution. She needs to find the osmolarity of a Phosphate Buffered Saline solution (made inhouse). She has the normal concentrations for all the components, but doesn't know where to go from there, and neither do I!!!! Any help or info is, as always, appreciated. Sandra Etheridge Animal Health Center BC Ministry of Agriculture, Food & Fisheries Abbotsford, BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Carolyn.Earley <@t> leica-microsystems.com Wed Jul 7 17:09:14 2004 From: Carolyn.Earley <@t> leica-microsystems.com (Carolyn.Earley@leica-microsystems.com) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] RE: osmolarity Message-ID: Definition: Osmolarity approximately equals the sum of the molarities of the particles in solution. Examples: a. a 0.1 M glucose has an approximate osmolarity of 0.1 OsM. b. a 0.3 M KCl has an approximate osmolarity of 0.6 OsM because the KCl will dissociate into 0.3 M K+ and 0.3 M Cl-. c. a 0.4 M MgCl2 has an approximate osmolarity of 1.2 OsM because the MgCl2 will dissociate into 0.4 M Mg++ and?2*0.4?M?Cl -. d. a solution containing 0.1 M glucose, 0.3 M KCl, and 0.4 M MgCl2 has an approximate osmolarity of 1.9 OsM (0.1 + 0.6 + 1.2). Carolyn Earley Marketing Manager Leica Microsystems 847-405-7014 Carolyn.Earley@Leica-Microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From lpwenk <@t> sbcglobal.net Wed Jul 7 19:23:52 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] nissl strain References: <1089212210.40ec0f32bca35@mail.umich.edu> Message-ID: <005101c46481$da153c00$86d3d445@domainnotset.invalid> The tissue is drying out. To prevent this, apply a synthetic mounting media BEFORE the xylene evaporates, and cover with a coverslip of glass or acetate tape. The synthetic mounting media will dry, permanently attaching the coverslip to the slide and tissue (so you can't accidentally scratch off the tissue). AND, the great bonus, the tissue will look "wet" all the time, so the stain and image will look like it is supposed to look. Same reason why every H&E and every special stain and IHC stain are coverslipped. In microbiology and hematology, they can get away with not coverslipping stains like giemsa on blood smears and auramine-rhodamine on smears from bronchus mucus. That is because they are working with SMEARS of individual cells or micro-organisms. In histology, we are working with fixed and processed tissues, with cells that are have been compromised by all the chemicals, and that need to be coverslipped to look right. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Wednesday, July 07, 2004 10:56 AM Subject: [Histonet] nissl strain > i did a nissl stain w. cresyl violet. after my last xylene step i have been > letting the slides dry off, and i visulize them (w. out a cover slip, these > slides will be used for LCM), and the slides look great. > > if after about 5 min of drying i look at them, all of a sudden this darkness > overcomesthe slide and the tissue looks black in color. i can actauly view the > blackness overcome the slide. > > then if i redip it in xylene, the blackness goes awya and the slides look awsome > again. i find if i cover slip the slides prior to teh blackness, they preserve > in the good looking state. why is this happening? how can i stop it from > happening w/ our a cover slip? wat is going on here? > > someone in my lab suggested oxidation, but i am not quite sure wat thta is all > about. > > -mike- > ann arbor > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Jul 7 19:30:13 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] HT's must be registered? References: <9AE8AA9E1F644B4AA6C155FB6FD51C6322B8DD@EMAIL.archildrens.org> Message-ID: <005a01c46482$bc900e20$86d3d445@domainnotset.invalid> The ONLY thing that is changing is that the ASCP Board of Registry, as of Jan 1, 2005, will no longer have the HT route of high school diploma and 2 year on the job (OJT) training. That route will be dropped. That will leave the other two routes - Associate degree with 20 credits of biology and chemistry with 1 year OJT, OR, successful completion of a NAACLS accredited HT program. There is no requirement by ASCP or NSH or anyone else, that says that histotechs MUST be registered in the future (except for Florida, which requires state licensure or ASCP certification). On the other hand, hospitals or the labs can make their own requirements. Maybe their lab manager/supervisor/pathologists have decided they only want ASCP certified people. Sure makes it easier to "prove" to CAP, JCAHO, and any court that is suing a lab, that the people working in the lab are qualified to work in that lab and perform those tests. (Wonder how often my head will be chewed off over this remark?) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Horn, Hazel V" To: Sent: Wednesday, July 07, 2004 10:15 AM Subject: [Histonet] HT's must be registered? I have been asked this question now twice. They are wanting to know if HT's have to be registered. Is it required by law, CAP, or any other authority? I know we would love for all techs to be registered but I didn't think it was mandatory? Thanks as always for your help. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rschoon <@t> email.unc.edu Wed Jul 7 19:34:31 2004 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene Substitutes In-Reply-To: References: Message-ID: <40EC9697.5080306@email.unc.edu> Jeff, If you were a male rat, not of the NBR strain as as you wouldn't have the alpha 2u globulin (a-2uG) producing gene, you could develop kidney cancer as d-limonene reversably binds to a-2uG and causes a variety of intra and extra cellular changes which will eventually lead to kidney carcinogenesis. No other organs are involved, female rats are not affected, neither are mice although the males produce a close analog. Humans do not and are thus not affected. That said ....I am one of those that the smell of d-limonene makes nauseous. Many of the early studies were performed by my boss Dr. James A Swenberg and lately (last 10 years) by Dr. Swenberg and myself (industry reports, EPA & IRAC). You really don't have a lot to worry about cancer wise but the smell certainly affects some people more than others. Not in my office so the exact reff's are not at hand. Robert Schoonhoven Jeffrey Thompson wrote: > Hello Histonetters, > > This may be an old, tired, and oft asked question, but what ARE the > health risks of the d-limonene containing xylene alternatives? The > butylated hydroxyanisole in Fisher's CitriSolv is a carcinogen 'suspect' > and there are many MSDS mentions of the possible allergen status of > d-limonene. Many also are the stories of headaches caused by the stuff. > Biodegradable qualities notwithstanding, I personally don't think these > products are as 'mother's milk' safe as some seem to think (I don't > think they should be poured down the sink for instance). As a group who > may use these products more and in a greater volume than any other, I'd > like to hear what the histology community thinks about use, handling, > and disposal regardless of what the various MSDS's have to say. Thanks, > > Jeff > > Jeffrey F. Thompson > Associate Scientist > University of New Mexico > Health Sciences Center > Department of Neurology > BRF Room 136 > 915 Camino de Salud NE > Albuquerque, NM 87131 > USA > > jefthompson@salud.unm.edu > > Phone: (505) 272-8010 > FAX: (505) 272-0607 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elyse.Keon <@t> sdcounty.ca.gov Wed Jul 7 18:24:35 2004 From: Elyse.Keon <@t> sdcounty.ca.gov (Keon, Elyse ) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] manual for the fisher 266MP Message-ID: Hi Ze Lu I actually have an Instruction Manual for the Fisher tissue processor Model 266MP. It's a 56 page book that I would be happy to copy for you. Just let me know where to send it. Laurie Pereira email: laurie.pereira@sdcounty.ca.gov From leclercj <@t> vetanat.unizh.ch Thu Jul 8 01:52:31 2004 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Cox-IHC in uterus Message-ID: Good morning! I have problems with performing COX-2-immunohistochemistry on bovine uterus. I haven`t found an adequate antibody yet. Those that I tried showed excessive unspecific bindings (Merck Frost) respectively didn`t bind at all (BD Transduction labs, mouse monoclonal clone 33). Additionally, employing a Vectastain ABC Universal Elite Kit (rabbit and mouse IgG) I have severe problems with background, especially in the perimetrium and connective tissue. Seems like the secondary antibody cross-reacts with the bovine tissue although I did peroxidase block (0.3% in methanol for 30 min), avidin-biotin block (Vector), the normal serum which came along with the Vectastain kit and sometimes tried an additional protein block (Dako). I would be very happy if anyone of you out there could share the experience with me and help with finding an antibody an eliminating the "universal-kit-problems". Thank you very much. Jocelyne Leclerc ____________________________________ Jocelyne Leclerc Institute for Veterinary Anatomy Vetsuisse Faculty, University of Zurich Switzerland From mab70 <@t> medschl.cam.ac.uk Thu Jul 8 02:09:23 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] knives and scissors with disposable blades? Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111366@mius2.medlan.cam.ac.uk> Serotec does disposable autopsy knives, I don't know about the scissors though. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, July 06, 2004 5:29 PM To: histonet@pathology.swmed.edu Subject: [Histonet] knives and scissors with disposable blades? We have a new pathologist fresh out of school. She tells me the place she was a resident (somewhere in Texas) had long knives and scissors with disposable blades. Can any one help me out with this. I have searched the internet but did not come up with anything. Thanks. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Walker <@t> sanofi-synthelabo.com Thu Jul 8 03:08:39 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:23:42 2005 Subject: =?ISO-8859-1?Q?R=E9f=2E_=3A_[Histonet]_Cox-IHC_in_uterus_-pity_to_our?= =?ISO-8859-1?Q?_virgil_ears?= Message-ID: Finding this email on my computer this morning I wonder if you Anat. Path realise what it looks like you are doing!!!!!!!!!!!!!!!!!!!!!!! ---------------------------------------------------------- Le pr?sent message, ainsi que les pi?ces ou annexes qui s'y trouvent ?ventuellement jointes, s'adressent exclusivement ? celles des personnes qu'ils d?signent comme destinataires. Constituant de ce fait une correspondance priv?e ? caract?re confidentiel, leur contenu est prot?g? par le secret des correspondances ?mises, transmises ou re?ues par la voie des t?l?communications. Si ce message ?lectronique vous est parvenu fortuitement, veuillez avoir l'obligeance de le d?truire, puis d'en aviser l'exp?diteur dans les meilleurs d?lais. The Information in this e-mail belongs to Sanofi-Synthelabo, is intended for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential, or exempt from disclosure under applicable law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of, or reliance on, the contents of this e-mail is prohibited. If you have received this e-mail in error, please notify us immediately by replying back to the sending e-mail address, and delete this e-mail message from your computer. ---------------------------------------------------------- From d.borthwick <@t> dri-dna.co.uk Thu Jul 8 04:03:26 2004 From: d.borthwick <@t> dri-dna.co.uk (Duncan Borthwick) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Looking to buy mouse blocks in the UK?? Message-ID: Working on a project to develop a new protocol to purify DNA from fixed tissue in wax blocks and I don't have access to blocks of tissue. Therefore, I'm trying to find a source (preferably in the UK) who could supply fixed and embedded wax blocks of mouse tissu. Does anyone know of any? thanks D B From BMolinari <@t> heart.thi.tmc.edu Thu Jul 8 06:32:37 2004 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Gayles article Message-ID: Hi, Thank you Gayle Callis for your article in the most recent Polyfacts. The article deals with microtomy and Mega-Cassettes. I have had the same problem and will give your method a try. Thanks for sharing! Betsy Molinari HT(ASCP) Texas Heart Institute Houston, Texas 77030 832-355-6524 From Nancy.Walker <@t> sanofi-synthelabo.com Thu Jul 8 06:34:02 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:23:42 2005 Subject: =?ISO-8859-1?Q?R=E9f=2E_=3A_[Histonet]_Looking_to__buy_mouse_blocks_in?= =?ISO-8859-1?Q?_the_UK=3F=3F?= Message-ID: Hi I've bought mouse tissue in microarrays from Menarini, a french distributer of BIOGENEX products. "Eric Mariel is their nice technical salesman, don't know how his engish is : 330156451160, cell phone: 33672870298 emariel@diagnostic.menarini.fr yours truely, Nancy Walker Molecular Pharmacology Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 ---------------------------------------------------------- Le pr?sent message, ainsi que les pi?ces ou annexes qui s'y trouvent ?ventuellement jointes, s'adressent exclusivement ? celles des personnes qu'ils d?signent comme destinataires. Constituant de ce fait une correspondance priv?e ? caract?re confidentiel, leur contenu est prot?g? par le secret des correspondances ?mises, transmises ou re?ues par la voie des t?l?communications. Si ce message ?lectronique vous est parvenu fortuitement, veuillez avoir l'obligeance de le d?truire, puis d'en aviser l'exp?diteur dans les meilleurs d?lais. The Information in this e-mail belongs to Sanofi-Synthelabo, is intended for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential, or exempt from disclosure under applicable law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of, or reliance on, the contents of this e-mail is prohibited. If you have received this e-mail in error, please notify us immediately by replying back to the sending e-mail address, and delete this e-mail message from your computer. ---------------------------------------------------------- From mward <@t> wfubmc.edu Thu Jul 8 07:36:04 2004 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Rick antibody Message-ID: <61135F0455D33347B5AAE209B903A304076A4EBA@EXCHVS2.medctr.ad.wfubmc.edu> I have been told recently that the R. ricksetti antibody we usually buy from the CDC is unavailable. (I'm not sure if this is temporary or not.) Does anyone have another vendor for this antibody? Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From dobbin <@t> upei.ca Thu Jul 8 07:52:35 2004 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Cox-IHC in uterus In-Reply-To: Message-ID: <40ED1962.13963.3FD970@localhost> Hi Jocelyne, Here is my 2 cents worth: a) For the Ab's that are not working, try incubating the primary overnight in the fridge before giving up on them. b) For the one with excessive background, try adding (up to) 1% bovine serum albumin (or normal bovine serum) to the primary (make sure you treat your negative controls the same). This might adsorb out the (or some of the) non-specific rx. c) If you really want to try the procedure without the universal kit, go for it. Just use goat anti-mouse or rabbit anti-mouse conjugated with peroxidase diluted to approx. 1:200. I do think however you will be sacrificing some sensitivity in the test. Good luck. Greg Date sent: Sat, 15 Jan 2005 05:24:16 +0100 From: Leclerc Jocelyne To: Subject: [Histonet] Cox-IHC in uterus > Good morning! > I have problems with performing COX-2-immunohistochemistry on bovine uterus. > > I haven`t found an adequate antibody yet. Those that I tried showed > excessive unspecific bindings (Merck Frost) respectively didn`t bind at all > (BD Transduction labs, mouse monoclonal clone 33). > Additionally, employing a Vectastain ABC Universal Elite Kit (rabbit and > mouse IgG) I have severe problems with background, especially in the > perimetrium and connective tissue. > Seems like the secondary antibody cross-reacts with the bovine tissue > although I did peroxidase block (0.3% in methanol for 30 min), avidin-biotin > block (Vector), the normal serum which came along with the Vectastain kit > and sometimes tried an additional protein block (Dako). > > I would be very happy if anyone of you out there could share the experience > with me and help with finding an antibody an eliminating the > "universal-kit-problems". > Thank you very much. > > Jocelyne Leclerc > > > ____________________________________ > Jocelyne Leclerc > Institute for Veterinary Anatomy > Vetsuisse Faculty, University of Zurich > Switzerland > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Greg Dobbin Pathology Lab Atlantic Veterinary College, U.P.E.I. 550 University Ave. Charlottetown, P.E.I. Canada, C1A 4P3 Phone: (902)566-0744 Fax: (902)566-0851 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Making a living is getting; making a life is giving. From froyer <@t> bitstream.net Thu Jul 8 07:49:15 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] knives and scissors with disposable blades? In-Reply-To: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111366@mius2.medlan.cam.ac.uk> References: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111366@mius2.medlan.cam.ac.uk> Message-ID: <40ED42CB.4050904@bitstream.net> Contact your local Sakura Rep., or call Sakura Finetek directly 800-725-8723. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, llc. Minneapolis, MN 55427 800-565-1895, ext. 17 Margaret Blount wrote: >Serotec does disposable autopsy knives, I don't know about the scissors >though. > >Margaret > >Margaret Blount >Chief Technician >Clinical Biochemistry >University of Cambridge >Addenbrooke's Hospital >Hills Road >Cambridge >CB2 2QR > >-----Original Message----- >From: Horn, Hazel V [mailto:HornHV@archildrens.org] >Sent: Tuesday, July 06, 2004 5:29 PM >To: histonet@pathology.swmed.edu >Subject: [Histonet] knives and scissors with disposable blades? > > >We have a new pathologist fresh out of school. She tells me the place >she was a resident (somewhere in Texas) had long knives and scissors >with disposable blades. Can any one help me out with this. I have >searched the internet but did not come up with anything. >Thanks. > > >Hazel Horn, HT/HTL (ASCP) >Histology Supervisor >Arkansas Children's Hospital > >Phone - 501.364.4240 >Fax - 501.364.3912 > > > > >---------------------------------------------------------------------------- >-- >The information contained in this message may be privileged and confidential >and protected from disclosure. If the reader of this message is not the >intended recipient, or an employee or agent responsible for delivering this >message to the intended recipient, you are hereby notified that any >dissemination, distribution or copying of this communication is strictly >prohibited. If you have received this communication in error, please notify >us immediately by replying to the message and deleting it from your >computer. Thank you. >============================================================================ >== >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From hagler.herb <@t> pathology.swmed.edu Thu Jul 8 08:19:16 2004 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Listserver notes from the admins Message-ID: <699AF09C-D0E1-11D8-99C6-000A95D9F7BA@pathology.swmed.edu> All, The problem with the multiple messages was due to one of the list subscribers having a badly configured computer/mail server that was reflecting all received messages immediately back to the server. It took about a week to identify which subscriber it was. There was no clue in the messages themselves. A round of applause to Kenneth Simmons who is responsible for the list server hardware at UT Southwestern helping me solve the problem. Advice for the future: Please, please handle your own subscribes and unsubscribes by using the web interface for controlling your own list server changes. The link for this is at the bottom of each and every message. You also receive a message once a month telling you the email address you are using on the server and your password. Please save this or write the information down. The link is: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please unsubscribe or check the option of no mail before you set an out of office reply. The list does not care (other than hoping you have a nice vacation) nor need to know that you are out of your office. This "feature" also causes lots of bounced messages and repetative messages. Each posting generates a reply and it really is annoying to everyone.... If you notice that you are not receiving messages please login to the web address and check the status of your subscription. When your mailbox is full especially hotmail and yahoo accounts, the messages bounce back to the list server. After 5 bounces detected your account is automatically placed in a no mail status and you have to log in to the list server to change this back to receiving mail. If you place subscribe and unsubscribe messages in the email they will be returned to you asking you to use the web interface. Linda and I do not always have the time to handle these manually, the list is just too large now for much hand holding by us. Thanks for your support during this latest round of problems Herb and Linda From Charles.Embrey <@t> carle.com Thu Jul 8 08:36:52 2004 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] HT's must be registered? Message-ID: Here we will only pay the employee as a histotech if they have passed the ASCP/BOR exam. Until that time they are paid as and considered a lab assistant. We currently don't have a license requirement in Illinois but one does seem to be in the works. To place the initials HT after your name I believe requires recognized certification. Chuck Embrey Urbana IL -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, July 07, 2004 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT's must be registered? I have been asked this question now twice. They are wanting to know if HT's have to be registered. Is it required by law, CAP, or any other authority? I know we would love for all techs to be registered but I didn't think it was mandatory? Thanks as always for your help. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histolog <@t> fcv.unl.edu.ar Thu Jul 8 09:01:25 2004 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Room Temperature In-Reply-To: <699AF09C-D0E1-11D8-99C6-000A95D9F7BA@pathology.swmed.edu> Message-ID: <002201c464f4$0f7d8a60$5a0cd2aa@unl.edu.ar> Hello, everyone, I need know that range of temperature defines you as room temperature. Thanks Hugo ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From Terry.Marshall <@t> rothgen.nhs.uk Thu Jul 8 08:59:02 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Listserver notes from the admins Message-ID: Herb - you could be deserving of a Nobel prize for restraint comment. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Herb Hagler [mailto:hagler.herb@pathology.swmed.edu] Sent: 08 July 2004 14:19 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Listserver notes from the admins All, The problem with the multiple messages was due to one of the list subscribers having a badly configured computer/mail server that was reflecting all received messages immediately back to the server. It took about a week to identify which subscriber it was. There was no clue in the messages themselves. A round of applause to Kenneth Simmons who is responsible for the list server hardware at UT Southwestern helping me solve the problem. Advice for the future: Please, please handle your own subscribes and unsubscribes by using the web interface for controlling your own list server changes. The link for this is at the bottom of each and every message. You also receive a message once a month telling you the email address you are using on the server and your password. Please save this or write the information down. The link is: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please unsubscribe or check the option of no mail before you set an out of office reply. The list does not care (other than hoping you have a nice vacation) nor need to know that you are out of your office. This "feature" also causes lots of bounced messages and repetative messages. Each posting generates a reply and it really is annoying to everyone.... If you notice that you are not receiving messages please login to the web address and check the status of your subscription. When your mailbox is full especially hotmail and yahoo accounts, the messages bounce back to the list server. After 5 bounces detected your account is automatically placed in a no mail status and you have to log in to the list server to change this back to receiving mail. If you place subscribe and unsubscribe messages in the email they will be returned to you asking you to use the web interface. Linda and I do not always have the time to handle these manually, the list is just too large now for much hand holding by us. Thanks for your support during this latest round of problems Herb and Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Thu Jul 8 09:09:49 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Listserver notes from the admins Message-ID: Bums! Got caught between two stools. I meant either: Herb - you could be deserving of a Nobel prize for restraint. or Herb - you could be deserving of a Nobel prize for restrained comment. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist Sent: 08 July 2004 14:59 To: Herb Hagler; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Listserver notes from the admins Herb - you could be deserving of a Nobel prize for restraint comment. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Herb Hagler [mailto:hagler.herb@pathology.swmed.edu] Sent: 08 July 2004 14:19 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Listserver notes from the admins All, The problem with the multiple messages was due to one of the list subscribers having a badly configured computer/mail server that was reflecting all received messages immediately back to the server. It took about a week to identify which subscriber it was. There was no clue in the messages themselves. A round of applause to Kenneth Simmons who is responsible for the list server hardware at UT Southwestern helping me solve the problem. Advice for the future: Please, please handle your own subscribes and unsubscribes by using the web interface for controlling your own list server changes. The link for this is at the bottom of each and every message. You also receive a message once a month telling you the email address you are using on the server and your password. Please save this or write the information down. The link is: http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please unsubscribe or check the option of no mail before you set an out of office reply. The list does not care (other than hoping you have a nice vacation) nor need to know that you are out of your office. This "feature" also causes lots of bounced messages and repetative messages. Each posting generates a reply and it really is annoying to everyone.... If you notice that you are not receiving messages please login to the web address and check the status of your subscription. When your mailbox is full especially hotmail and yahoo accounts, the messages bounce back to the list server. After 5 bounces detected your account is automatically placed in a no mail status and you have to log in to the list server to change this back to receiving mail. If you place subscribe and unsubscribe messages in the email they will be returned to you asking you to use the web interface. Linda and I do not always have the time to handle these manually, the list is just too large now for much hand holding by us. Thanks for your support during this latest round of problems Herb and Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kconway2 <@t> uwo.ca Thu Jul 8 09:29:32 2004 From: kconway2 <@t> uwo.ca (Kevin Conway) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] re: freeware to measure areas In-Reply-To: <200407081415.i68EFdCV026857@klik.its.uwo.ca> Message-ID: Hi, I'm in the process of writing a series of macros for Scion Image that can be used for this purpose. they are still a little rough around the edges, and I have to write up some documentation for them. They can be had at https://sourceforge.net/projects/imageapps/ Just load them as macros into Scion image, you can load multiple image and process them all with the same threshold. Thresholding is still manual (as in you pick a number between 0 and 255). Let me know if I can help out at all. Kevin Conway PhD Candidate Department of Anatomy & Cell Biology University of Western Ontario From jaimie_hk <@t> yahoo.co.uk Thu Jul 8 09:36:11 2004 From: jaimie_hk <@t> yahoo.co.uk (=?iso-8859-1?q?Jaimie=20Hoh?=) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval - Microwave problem Message-ID: <20040708143611.1233.qmail@web25001.mail.ukl.yahoo.com> Hi Histonet, I have a slight problem. I am perfoming Antigen retrieval using microwave boiling in Immunocytochemistry. When I put the slides in the microwave and when the citrate buffer boils, the specimen comes out from the slide or it folds. Does anyone have an alternative to performing Antigen retrieval using microwave? thanks, Jaimie Research Officer Singapore Eye Research Institute ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com From Terry.Marshall <@t> rothgen.nhs.uk Thu Jul 8 09:48:33 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval - Microwave problem Message-ID: Jaimie, surely the heat and humidity in Singapore is such that you could merely hold the slide out of the window for a minute or two. In my visits, my molecules felt loose and wobbly the instant I walked out of Changi airport doors. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Jaimie Hoh [mailto:jaimie_hk@yahoo.co.uk] Sent: 08 July 2004 15:36 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval - Microwave problem Hi Histonet, I have a slight problem. I am perfoming Antigen retrieval using microwave boiling in Immunocytochemistry. When I put the slides in the microwave and when the citrate buffer boils, the specimen comes out from the slide or it folds. Does anyone have an alternative to performing Antigen retrieval using microwave? thanks, Jaimie Research Officer Singapore Eye Research Institute ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Jul 8 09:38:07 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] B-Plus substitute for B5 Message-ID: Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From je22r <@t> udcf.gla.ac.uk Thu Jul 8 09:55:01 2004 From: je22r <@t> udcf.gla.ac.uk (Julia Edgar) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] fixative for giemsa stain? Message-ID: <001201c464fb$8b9ae690$40e9d182@vet> Dear All I need to do a giemsa stain on some primary muscle cells. Can you tell me if I should fix the cells first, and if so, with which fixative. Thank you. Julia Julia Edgar (BSc, PhD) University of Glasgow Veterinary School From akbitting <@t> geisinger.edu Thu Jul 8 09:58:54 2004 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Chromavision Message-ID: Has anyone heard what's going on with Chromavision? Ventana was a pen-stroke away from buying them out, but that fell through. Now I hear that there may be a buy-out going on and that those of us who have contracts with them may lose service. Anyone hear anything? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From la.sebree <@t> hosp.wisc.edu Thu Jul 8 10:01:15 2004 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval - Microwave problem Message-ID: Hi Jaimie, Try bringing your slides and buffer to just under the boiling point in the microwave and then transferring them to a 93-95 degree C waterbath for the duration. Better yet, invest in either an electric rice steamer (such as Black & Decker makes here in the states) or an electric laboratory pressure cooker (such as Biocare Medical makes). The point is to avoid boiling the buffer as it tends to dislodge tissue from the slides. I assume you are using "plus" or charged slides; this is critical for tissue adherence in immunohistochemistry procedures. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Jaimie Hoh [mailto:jaimie_hk@yahoo.co.uk] Sent: Thursday, July 08, 2004 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval - Microwave problem Hi Histonet, I have a slight problem. I am perfoming Antigen retrieval using microwave boiling in Immunocytochemistry. When I put the slides in the microwave and when the citrate buffer boils, the specimen comes out from the slide or it folds. Does anyone have an alternative to performing Antigen retrieval using microwave? thanks, Jaimie Research Officer Singapore Eye Research Institute ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Jul 8 10:01:50 2004 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Autopsy attendant training Message-ID: We are in need of a back up autopsy room attendant (deiner). I am under the impression that these people are usually trained on-the-job. But, I am wondering if anyone knows of a formal training program for this type of position? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Thu Jul 8 10:02:49 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] fixative for giemsa stain? Message-ID: Although the cells will fix as they stain, with the alcohol in the stain, the right thing to do is to fix in alcohol beforehand. 20 minutes in methanol is the usually quoted time for best results. The purists insist on methanol, though I have never been able to see the difference between that an ethanol. My and most others experience is with blood and marrow, but I guess a Giemsa is a Giemsa whatsoever it is staining. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Julia Edgar [mailto:je22r@udcf.gla.ac.uk] Sent: 08 July 2004 15:55 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] fixative for giemsa stain? Dear All I need to do a giemsa stain on some primary muscle cells. Can you tell me if I should fix the cells first, and if so, with which fixative. Thank you. Julia Julia Edgar (BSc, PhD) University of Glasgow Veterinary School _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Jul 8 10:10:00 2004 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Diastase digestion Message-ID: I've got a head-scratcher here. We stained three human liver cases with PAS w/ diastase. We mount our patient tissue on the same slide below the control tissue. We do the diastase digestion in a Coplin jar and all the slides were in the same jar. Our Pathologist showed me the slides. The glycogen was removed from the control tissue, but the test tissue was still positive. Does anyone have an idea what happened? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From azdudley <@t> hotmail.com Thu Jul 8 10:13:23 2004 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval - Microwave problem Message-ID: jamie, I use to do my retrieval in the microwave and if there was gelatin in the baths I had trouble with the tissue coming off. use + slides with no gelatin and you should have no problem. good luck! anita dudley, providence hosp. mobile alabama >From: Jaimie Hoh >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Antigen retrieval - Microwave problem >Date: Thu, 8 Jul 2004 15:36:11 +0100 (BST) > >Hi Histonet, > >I have a slight problem. > >I am perfoming Antigen retrieval using microwave >boiling in Immunocytochemistry. > >When I put the slides in the microwave and when the >citrate buffer boils, the specimen comes out from the >slide or it folds. > >Does anyone have an alternative to performing Antigen >retrieval using microwave? > >thanks, >Jaimie >Research Officer >Singapore Eye Research Institute > > > > > >___________________________________________________________ALL-NEW Yahoo! >Messenger - sooooo many all-new ways to express yourself >http://uk.messenger.yahoo.com > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar – get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ From livieira <@t> ualg.pt Thu Jul 8 10:33:07 2004 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] xilene substitutes Message-ID: <008801c46500$de342060$3314100a@labhistologia> Hi everyone About the xilene substitutes, Are the Clear-Rite, Propar and so (aliphatic hydrocarbons...) more safe than d-limonene compounds? Thanks From akbitting <@t> geisinger.edu Thu Jul 8 10:44:46 2004 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] B-Plus substitute for B5 Message-ID: We use B-Plus Fix for all of our bone marrows. It's from BBC. Everyone has been happy with it. No mercury pigment removal!! their website is BBCus.com ....................................................................................................................... Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 >>> "Joe Nocito" 07/08/04 10:38AM >>> Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From cathy <@t> wasatchhisto.com Thu Jul 8 09:27:27 2004 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] stain kits for sale Message-ID: <00b401c464f7$b3147aa0$ef693442@selfuggnbwd3wt> Fellow Histonetters, I have 4 staining kits for sale. They were purchased from Master-Tech and are as follows: 1) AFB stain kit paid $68.00 2) Alcian blue pH 2.5 paid $120.00 3) PAS stain kit paid $90.50 4) Chandler's Retic paid $115.00 I purchased them for one of my employees who was going to take the HT exam in June. However, she quit without notice and these kits are totally worthless to my lab since we do not perform any of these stains. Make an offer. Cathy ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ From jengirl1014 <@t> yahoo.com Thu Jul 8 12:17:44 2004 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: xylene substitutes Message-ID: <20040708171744.80785.qmail@web60602.mail.yahoo.com> I'd also like to know the answer to this question. Message: 14 Date: Thu, 8 Jul 2004 16:33:07 +0100 From: "Lina Vieira" Subject: [Histonet] xilene substitutes To: "Histonet" Message-ID: <008801c46500$de342060$3314100a@labhistologia> Content-Type: text/plain; charset="iso-8859-1" Hi everyone About the xilene substitutes, Are the Clear-Rite, Propar and so (aliphatic hydrocarbons...) more safe than d-limonene compounds? Thanks Could someone please answer it for me, too? Thanks a bunch. --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - Send 10MB messages! From j_gorenstein <@t> yahoo.com Thu Jul 8 12:43:27 2004 From: j_gorenstein <@t> yahoo.com (Juile Gorenstein) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Sakura Tissue Processor Message-ID: <20040708174329.54903.qmail@web42007.mail.yahoo.com> Hello all, I was wondering if anyone has had problems with xylene fumes using Sakura VIP Tissue processor. Ours is currently sitting on a bench in a room with a snorkle right above is and the smell is still really bad and is causing headaches for people who try to work in that room. Does anyone have any suggestions? Thank you, Julie Novartis Institues for Biomedical Research Cambridge, MA --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - 100MB free storage! From c.m.vanderloos <@t> amc.uva.nl Thu Jul 8 13:06:12 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] RE: Antigen retrieval - Microwave problem Message-ID: <84129083f3a9.83f3a9841290@amc.uva.nl> Jamie, The problem of sections coming off is mostly confined to tissue sections that contain a lot of fat. Leaving the slides in a stove at 37C for a couple of days sometimes helps. Recently we have been successful with an overnight antigen retrieval procedure at 70C (citrate 6.0 or Tris/EDTA 9.0 whatever you prefer). The sections (also the real fatty ones) nicely stay on the slides and antigen retrieval is just as good. In some cases even more effective. Good luck, Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From Jaimie Hoh Date Thu, 08 Jul 2004 15:36:11 +0100 (BST) To histonet@lists.utsouthwestern.edu Subject [Histonet] Antigen retrieval - Microwave problem Hi Histonet, I have a slight problem. I am perfoming Antigen retrieval using microwave boiling in Immunocytochemistry. When I put the slides in the microwave and when the citrate buffer boils, the specimen comes out from the slide or it folds. Does anyone have an alternative to performing Antigen retrieval using microwave? thanks, Jaimie Research Officer Singapore Eye Research Institute From j_gorenstein <@t> yahoo.com Thu Jul 8 13:16:53 2004 From: j_gorenstein <@t> yahoo.com (Juile Gorenstein) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Sakura Tissue Processor Message-ID: <20040708181653.62735.qmail@web42007.mail.yahoo.com> Hello again, Thanks LuAnn for the suggestion. However, we just had this instrument brought out of storage and we put in fresh filter (2 weeks old) and we change water and everything. It is useful to know that it should not be doing this, and that we should not need a hood. Any further suggestions are welcome! Thanks again! Julie LuAnn Anderson wrote: Date: Thu, 08 Jul 2004 13:07:39 -0500 To: Juile Gorenstein From: LuAnn Anderson Subject: Re: [Histonet] Sakura Tissue Processor Hi Julie, Our VIP has a charcoal station in the very last reagent position. The activated charcoal helps to absorb xylene fumes. If you are not using charcoal in your VIP, or if it hasn't been changed in a while, I would recommend filling that container with fresh activated charcoal pieces (not powder). I think that I ordered it through Allegiance. It is Tissue Tech Activated carbon #4663. If you need me to, I can look up where I ordered and let you know for sure. Good luck. LuAnn At 10:43 AM 7/8/04 -0700, you wrote: >Hello all, >I was wondering if anyone has had problems with xylene fumes using Sakura >VIP Tissue processor. Ours is currently sitting on a bench in a room with >a snorkle right above is and the smell is still really bad and is causing >headaches for people who try to work in that room. Does anyone have any >suggestions? > >Thank you, >Julie > >Novartis Institues for Biomedical Research >Cambridge, MA > > >--------------------------------- >Do you Yahoo!? >New and Improved Yahoo! Mail - 100MB free storage! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! From histonet <@t> histonet.blogdns.com Thu Jul 8 13:27:41 2004 From: histonet <@t> histonet.blogdns.com (Judy Pariser) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Xylene Substitutes Message-ID: <000c01c46519$41b5ae70$0600a8c0@IN4> I am responding to the postings regarding xylene substitutes - specifically the d-Limonenes. I am the Project Manager for CBG Biotech's new clearing solvent, Formula 83, and perhaps I can shed some light on your questions. First, it is important to note that d-Limonene solvents that are sold for histological use are petroleum-based, and they therefore cannot be poured down the drain (as per the EPA and the Clean Water Act). Only solvents that are prepared entirely from plant and/or animal sources can be put down a drain. While adequate ventilation and proper handling are a must with solvents and chemicals, it is equally important to select solvents that OSHA has classified as non-toxic, thereby not requiring personal exposure limits. As has been well demonstrated, it is the double bonds in benzenoid compounds that cause the toxicity of solvents like xylene and toluene. Although the d-Limonene solvents are not as toxic as xylene, they still contain some double bonds. The only safer alternatives are solvents with NO double bonds. While there are quite a few safer solvents available, it then becomes important to find one that will perform up to your standards. The aliphatic hydrocarbon solvents, although non-toxic, do not have a central cyclohexane ring, and will not offer the performance of solvents with a central cyclohexane ring. Chemically, Formula 83 Clearing Solvent is a naphthenic solvent. As such, it does not have the toxicity associated with double bonds, while its performance is excellent due to its central cyclohexane ring. It outperforms xylene and other xylene substitutes in slide clarity, slide drying, paraffin-dissolving and personnel safety. It does not harden tissue as xylene does, and it has better lipid extraction. Additionally, most users notice a crisper, clearer nuclear detail with more vivid staining. I would be happy to share more information with you. I can be reached at the phone number or email address below. Best regards, Judy Pariser Project Manager Email: jpariser@cbgbiotech.com Phone: 800-941-9484 Fax: 614-863-1676 Website: www.cbgbiotech.com From JMacDonald <@t> mtsac.edu Thu Jul 8 14:01:41 2004 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Sakura Tissue Processor In-Reply-To: <20040708181653.62735.qmail@web42007.mail.yahoo.com> Message-ID: You mentioned that the unit just came out of strorage. Is it possible that some of the seals are dried out and allowing fumes to escape? Jennifer MacDonald Juile Gorenstein Sent by: histonet-bounces@lists.utsouthwestern.edu 07/08/2004 11:16 AM To Histonet cc Subject Re: [Histonet] Sakura Tissue Processor Hello again, Thanks LuAnn for the suggestion. However, we just had this instrument brought out of storage and we put in fresh filter (2 weeks old) and we change water and everything. It is useful to know that it should not be doing this, and that we should not need a hood. Any further suggestions are welcome! Thanks again! Julie LuAnn Anderson wrote: Date: Thu, 08 Jul 2004 13:07:39 -0500 To: Juile Gorenstein From: LuAnn Anderson Subject: Re: [Histonet] Sakura Tissue Processor Hi Julie, Our VIP has a charcoal station in the very last reagent position. The activated charcoal helps to absorb xylene fumes. If you are not using charcoal in your VIP, or if it hasn't been changed in a while, I would recommend filling that container with fresh activated charcoal pieces (not powder). I think that I ordered it through Allegiance. It is Tissue Tech Activated carbon #4663. If you need me to, I can look up where I ordered and let you know for sure. Good luck. LuAnn At 10:43 AM 7/8/04 -0700, you wrote: >Hello all, >I was wondering if anyone has had problems with xylene fumes using Sakura >VIP Tissue processor. Ours is currently sitting on a bench in a room with >a snorkle right above is and the smell is still really bad and is causing >headaches for people who try to work in that room. Does anyone have any >suggestions? > >Thank you, >Julie > >Novartis Institues for Biomedical Research >Cambridge, MA > > >--------------------------------- >Do you Yahoo!? >New and Improved Yahoo! Mail - 100MB free storage! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Thu Jul 8 14:11:48 2004 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Sakura Tissue Processor In-Reply-To: <20040708174329.54903.qmail@web42007.mail.yahoo.com> References: <20040708174329.54903.qmail@web42007.mail.yahoo.com> Message-ID: <40ED9C74.6080303@rci.rutgers.edu> What model of VIP? We have a VIP 5, and so far we have had no problems with fumes from it. Our processor has a water jug for fume control (which needs to be changed daily), plus an activated carbon cartridge that needs to be changed every so often (I forget what the manual recommends, but it's in there). If your processor has these features, when was the last time you changed them? Kathleen Roberts Neurotoxicology Labs Rutgers University Juile Gorenstein wrote: >Hello all, >I was wondering if anyone has had problems with xylene fumes using Sakura VIP Tissue processor. Ours is currently sitting on a bench in a room with a snorkle right above is and the smell is still really bad and is causing headaches for people who try to work in that room. Does anyone have any suggestions? > >Thank you, >Julie > >Novartis Institues for Biomedical Research >Cambridge, MA > > >--------------------------------- >Do you Yahoo!? >New and Improved Yahoo! Mail - 100MB free storage! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Jason.Wiese <@t> med.va.gov Thu Jul 8 14:19:38 2004 From: Jason.Wiese <@t> med.va.gov (Wiese, Jason VHAROS) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Sakura Tissue Processor Message-ID: My thoughts exactly Jennifer! Jason Wiese, HT(ASCP) Roseburg, Oregon -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Thursday, July 08, 2004 12:02 PM To: Juile Gorenstein Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Tissue Processor You mentioned that the unit just came out of strorage. Is it possible that some of the seals are dried out and allowing fumes to escape? Jennifer MacDonald Juile Gorenstein Sent by: histonet-bounces@lists.utsouthwestern.edu 07/08/2004 11:16 AM To Histonet cc Subject Re: [Histonet] Sakura Tissue Processor Hello again, Thanks LuAnn for the suggestion. However, we just had this instrument brought out of storage and we put in fresh filter (2 weeks old) and we change water and everything. It is useful to know that it should not be doing this, and that we should not need a hood. Any further suggestions are welcome! Thanks again! Julie LuAnn Anderson wrote: Date: Thu, 08 Jul 2004 13:07:39 -0500 To: Juile Gorenstein From: LuAnn Anderson Subject: Re: [Histonet] Sakura Tissue Processor Hi Julie, Our VIP has a charcoal station in the very last reagent position. The activated charcoal helps to absorb xylene fumes. If you are not using charcoal in your VIP, or if it hasn't been changed in a while, I would recommend filling that container with fresh activated charcoal pieces (not powder). I think that I ordered it through Allegiance. It is Tissue Tech Activated carbon #4663. If you need me to, I can look up where I ordered and let you know for sure. Good luck. LuAnn At 10:43 AM 7/8/04 -0700, you wrote: >Hello all, >I was wondering if anyone has had problems with xylene fumes using Sakura >VIP Tissue processor. Ours is currently sitting on a bench in a room with >a snorkle right above is and the smell is still really bad and is causing >headaches for people who try to work in that room. Does anyone have any >suggestions? > >Thank you, >Julie > >Novartis Institues for Biomedical Research >Cambridge, MA > > >--------------------------------- >Do you Yahoo!? >New and Improved Yahoo! Mail - 100MB free storage! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jul 8 14:25:06 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] xylene substitutes vs limonene Message-ID: <3.0.6.32.20040708132506.00c106d0@gemini.msu.montana.edu> In my lab we consider them safer but one must still adhere to safety in handling, MSDS will give information or talk to technical services at companies who sell them if MSDS is too brief - I am not sure the whole answer about safety of xylene subtitutes (single aliphatic hydrocarbons) is totally known - this is the impression I get from answers given over the years on Histonet. We do use them, happily and with good results. People can become very sensitive to limonene compounds. I met one lady so allergic to them, she passed out when she smelled them. I turn a rather sickly shade of green and totally nauseous. The fact that people get sick (Bob Schoonhoven reply this week) when around these stinky, foul smelling (my candid opinion!) substitutes is the one reason they are banned from our laboratory. If they make you sick, then are they safe? I would think the answer would be - NO! Since they are found in some "grease cutting" household cleaning products, labels are examined and I have been known to open containers to detect odorous presence of limonene. Buyer beware! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From neuroant <@t> hotmail.com Thu Jul 8 14:31:53 2004 From: neuroant <@t> hotmail.com (Ant S.) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] ASCP Exam Message-ID: I'm hoping to take the Board of Registry Exam this fall, and when I submitted my application, I had to note how much on the job training (OJT) I had by the date of application (*not the date of the exam!*) Unfortunatly, as of the application date, I do not have enough to qualify for the upcoming exam. (I also do not have the appropriate schooling, so currently, OJT, also known as "Route 3", is my only option.) Does this mean that I can not try for any future exams unless I have schooling? I am confused if the cut-off date (1/1/05) for Route 3 is for the application date or for the testing date? Is this upcoming fall/winter exam my last chance, before I have to heist myself back to school? Thanks in advance, Ant Antoinette Swensson Univeristy of Washington/Harborview Medical Center Neuropathology (206) 731-3910 _________________________________________________________________ [1]MSN Life Events gives you the tips and tools to handle the turning points in your life. References 1. http://g.msn.com/8HMBENUS/2743??PS=47575 From JPCOLEMA <@t> sentara.com Thu Jul 8 15:34:46 2004 From: JPCOLEMA <@t> sentara.com (JOHN COLEMAN) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] recycler, nissl slides, m-wave slides Message-ID: We found the Xylene out of the recycler is more pure than what is available commercially. We used our recycled xylene for processing, staining but not coverslipping on an automatic celluloid tape coverslipper b/c the recycled xylene distorts the tape. we had both the CBG and the BR in at the same time, did a side by side analysis and the CBG hands down was a better, easier, safer instrument and if not for a tech spilling some xylene on the floor we would still be using it. A great feature of the CBG instrument was that it didn't raise the room temp at all. ( space here being at a premium) your slide is just drying out. the "blackness" is the dry tissue and stain absorbing rather than reflecting light, cresyl violet being relatively dark. Your tissue won't be ruined and as you notice, just dunk back in xylene and voila- good as new. If you don't want a coverslip(?) you can add a few drops of immersion oil and the slide will look just as nice. Coverslipping is a protective measure though, so why not? Just be careful not to scratch the cell off if you don't cover 'em. I work in a high volume IHC lab and we float 4 micron thick sections on a tap water bath at 45C, pick up on poly-l lysine or aminosilane slides, place label end down in a polyethylene microwaving tray , microwave on high in an 1100 w oven for 3 minutes, cool and procede.For IHC we use heat retrieval on all but 2 of our 120 antibodies. The timing is different according to your oven and # of slides, and you MUST have a functional carousel tray,(ever see a slide explode?) Our routine lab treats all the routine slides in the same manner with great results. They only use treated slides for certain procedures. From Diane.Gladney <@t> se.amedd.army.mil Thu Jul 8 15:12:24 2004 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] B-Plus substitute for B5 Message-ID: <9D41AB7C56F8304F98537ABD87B249F662622B@dasmthgbz001.amedd.army.mil> Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Thu Jul 8 16:21:48 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval - Microwave problem Message-ID: <642482973.1089321708254.JavaMail.osg@spnode33> Hi Jaime! I can definitely sympathize. I was having the same problem with slides I'm using for immunohistochemistry (subbed with poly-l-lysine, and I put gelatin in the waterbath). What I ended up doing is taking buffer in a coplin jar (i use 8.5 pH .5M tris) putting it in the microwave, and measuring the temperature in the jar every 10 sec. Tedious I know, but it gave me a good idea of how long it takes to boil and how hot it is when it is boiling, so I was able to choose a time that was just before the boiling point. I put my slides in a coplin jar in a small water bath in the microwave for 45 sec and then repeat emptying and refilling the water. Good luck! -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From caroline.stott <@t> anatomy.otago.ac.nz Thu Jul 8 16:58:36 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] RE: Antigen retrieval - Microwave problem Message-ID: <5.2.1.1.0.20040709095514.02e06ba0@anatomy.otago.ac.nz> Hi Jamie, We had big problems using breast tissue and sections boiling off the slide. We ended up using superfrost plus slides, and bringing the citrate buffer to boil then leaving the tissue for 5 minutes. We did this 3 times and the results were good. The sections definitely held on better. Also leaving the sections over night in a 37 or 56 degree incubator helped. Good luck. Caroline Caroline Stott Histology Service Unit University of Otago PO Box 913 Dunedin, New Zealand Ph (03) 479 7152 Fax (03) 479 7136 From carl.hobbs <@t> kcl.ac.uk Thu Jul 8 16:51:54 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] re-strained stools Message-ID: <001701c46535$c8f22140$68ed9b51@home> I sincerely hope they were quite compacted, Terry.....could be a rather messy faut pas otherwise ;-))) --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 From katri <@t> cogeco.ca Thu Jul 8 17:02:50 2004 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Diastase digestion References: Message-ID: <007801c46537$4fe2d950$6a9a9618@Katri> Hi Angela, Are you sure the positive staining in your test slide is glycogen? Liver cells can have all kinds of endogenous pigments, some of which are Pas positive. Katri Katri Tuomala St.Joseph's Health Care Hamilton, Ontario, Canada ----- Original Message ----- From: "Angela Bitting" To: Sent: Thursday, July 08, 2004 11:10 AM Subject: [Histonet] Diastase digestion I've got a head-scratcher here. We stained three human liver cases with PAS w/ diastase. We mount our patient tissue on the same slide below the control tissue. We do the diastase digestion in a Coplin jar and all the slides were in the same jar. Our Pathologist showed me the slides. The glycogen was removed from the control tissue, but the test tissue was still positive. Does anyone have an idea what happened? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lance.erickson <@t> ihc.com Thu Jul 8 17:24:25 2004 From: lance.erickson <@t> ihc.com (Lance Erickson) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Utah Society for Histotechnology's Annual Symposium Message-ID: The Utah Society for Histotechnology's annual symposium will be held August 6th and 7th at Primary Children's Medical Center in Salt Lake City. More information including program overview, exhibitors, and a complete downloadable program including registration form is available at www.utahhisto.org by choosing the meeting/events link. Any additional requests for an electronic program or snail mail version or questions in general about the program can be sent to me. Thanks Lance Erickson Anatomic Pathology Primary Children's Medical Center 100 N Medical Dr Salt Lake City, UT 84113 (801) 588-3110 From lpwenk <@t> sbcglobal.net Thu Jul 8 19:44:27 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] ASCP Exam References: Message-ID: <006a01c4654d$e4d70340$6ea6ff44@domainnotset.invalid> The 1/1/05 deadline is for the TAKING of the exam. The APPLICATION deadline to sign up for the Oct-Dec. 2004 cycle was July 1, 2004. So if you have not applied before July 1, 2004, it is now too late to sign up for the high school graduate/2 years on-the-job-training route for the HT exam. If you had applied before the 7/1/04 application deadline, but was rejected by ASCP Board of Registry (BOR) due to having less than 2 years experience, but you will have two years of experience in before the 1/1/05 deadline, you have nothing to lose by contacting ASCP BOR and asking them to review your case. Contact the ASCP at 312-738-1336, press "0" for operator, and ask for the Board of Registry. If you had not applied before the 7/1/05 application deadline, then I don't know of anything you can do to take the HT exam, except to earn an associate degree with 20 credits of biology and chemistry, or successfully complete a NAACLS histology program. One option might be Indiana University. Indiana U does it's training by teleconference and emails, and there are homework assignments and slides that must be turned in, and exams that must be taken under the watchful eye of a person in the lab who is a registered HT or HTL. For more information, go to http://www.indiana.edu/~bltindy/ahlt/histotech/cert.html Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Ant S." To: Sent: Thursday, July 08, 2004 3:31 PM Subject: [Histonet] ASCP Exam > > I'm hoping to take the Board of Registry Exam this fall, and when I > submitted my application, I had to note how much on the job training > (OJT) I had by the date of application (*not the date of the exam!*) > Unfortunatly, as of the application date, I do not have enough to > qualify for the upcoming exam. (I also do not have the appropriate > schooling, so currently, OJT, also known as "Route 3", is my only > option.) Does this mean that I can not try for any future exams > unless I have schooling? I am confused if the cut-off date (1/1/05) > for Route 3 is for the application date or for the testing date? Is > this upcoming fall/winter exam my last chance, before I have to heist > myself back to school? > > > Thanks in advance, > > Ant > > Antoinette Swensson > Univeristy of Washington/Harborview Medical Center > Neuropathology > (206) 731-3910 > _________________________________________________________________ > > [1]MSN Life Events gives you the tips and tools to handle the turning > points in your life. > > References > > 1. http://g.msn.com/8HMBENUS/2743??PS=47575 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WWmn916 <@t> aol.com Thu Jul 8 22:20:12 2004 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] slide labeling system or software Message-ID: <78.5b3fee69.2e1f68ec@aol.com> Hello fellow histo folks, Would anyone have good recomendations for a slide labeling system or software? I recently saw in the Fisher catalog a unit that cost about $650.00 and incorporates using bar codes. Has anyone used this and is it worth the money? Is a bar code labeling faster than using computer program and printing labels? Any recomendations for labeling H+E slides is appreciated. Thanks:-) Deb King, HT (ASCP) Sacramento, CA From mab70 <@t> medschl.cam.ac.uk Fri Jul 9 01:55:55 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval - Microwave problem Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211136D@mius2.medlan.cam.ac.uk> Are You using charged (Superfrost Plus or similar) slides? Also how do you dry your sections prior to dewaxing. Another point is to bring the buffer to boiling then turn down the power until it just maintains a gentle simmer, do not boil the sections vigourously. That's my two happenyworth. Regards Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Jaimie Hoh [mailto:jaimie_hk@yahoo.co.uk] Sent: Thursday, July 08, 2004 3:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval - Microwave problem Hi Histonet, I have a slight problem. I am perfoming Antigen retrieval using microwave boiling in Immunocytochemistry. When I put the slides in the microwave and when the citrate buffer boils, the specimen comes out from the slide or it folds. Does anyone have an alternative to performing Antigen retrieval using microwave? thanks, Jaimie Research Officer Singapore Eye Research Institute ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Fri Jul 9 07:06:25 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] B-Plus update Message-ID: I just wanted to thank everyone for their responses and experiences with B-Plus fixative. I've forwarded some of your replies to my hempath. You people are the best. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From jaimie_hk <@t> yahoo.co.uk Fri Jul 9 07:46:43 2004 From: jaimie_hk <@t> yahoo.co.uk (=?iso-8859-1?q?Jaimie=20Hoh?=) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Antigen retrieval-microwave problem Message-ID: <20040709124643.54289.qmail@web25003.mail.ukl.yahoo.com> Hi everybody, Thanks a lot for your great help. I used the poly-lysine slides and i have tried the waterbath at 95 degree celsius and it worked! That's great!! Best Regards, Jaimie ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com From tbailey <@t> uab.edu Fri Jul 9 08:01:31 2004 From: tbailey <@t> uab.edu (Tammy Bailey) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] UNSUBSCRIBE In-Reply-To: <20040709124643.54289.qmail@web25003.mail.ukl.yahoo.com> Message-ID: <001401c465b4$db344110$0f9c1a8a@tbailey1> Tammy M. Bailey University of Alabama-Birmingham Histology Analysis Module Manager Vision Science Research Center 924 18th St. South Birmingham, AL 35294 205.934.3074 205.934-5725-fax tbailey@uab.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jaimie Hoh Sent: Friday, July 09, 2004 7:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen retrieval-microwave problem Hi everybody, Thanks a lot for your great help. I used the poly-lysine slides and i have tried the waterbath at 95 degree celsius and it worked! That's great!! Best Regards, Jaimie ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Raoul.Regnault <@t> interiorhealth.ca Fri Jul 9 11:17:24 2004 From: Raoul.Regnault <@t> interiorhealth.ca (Regnault, Raoul) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Autopsy attendant training Message-ID: <1DA1FB459D6ED44EB804DB0C8BE230951B0669@dc1serv2> This community college in British Columbia offers a distance education certificate program called 'Pathology Attendant' and does cover the duties of this job function quite thoroughly. This is the relevant url: http://www.vcc.ca/programs/progDetail10.cfm?WPGM_DIVISION_ID=6&WPGM_PROGRAM_ ID=145 Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail B.C. 250-368-3311 ext 2263 fax 364-3421 raoul.regnault@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Autopsy attendant training We are in need of a back up autopsy room attendant (deiner). I am under the impression that these people are usually trained on-the-job. But, I am wondering if anyone knows of a formal training program for this type of position? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pam <@t> ategra.com Fri Jul 9 12:01:03 2004 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Histology professionals - Exciting new opportunities in the Big Apple!! Message-ID: Histonetters - I am presently on a search for one of my best clients in the greater NYC area who is seeking to hire a Lead Histotechnologist and an Immunohistochemistry Technologist. These are Full Time Permanent Positions. My client offers highly competitive salaries and excellent benefits. ***MON-FRI ONLY - D A Y S ONLY. (no working on weekends or holidays ! ) Are you interested ? Also, if you have friends/peers who might be interested, if you could pass my query & name on to them I'd be much obliged. If interested, please call me at 800-466-9919 x234 or e-mail me at pam@ategra.com Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: http://www.ategra.com Ategra Systems Inc Specialists in Permanent & Contract Staffing 7085 University Blvd Winter Park, FL 32792-6721 800-466-9919 ext 234 From tony.j.savage <@t> gsk.com Fri Jul 9 12:41:44 2004 From: tony.j.savage <@t> gsk.com (tony.j.savage@gsk.com) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] Message-ID: From: Juile Gorenstein wrote "Hello all, I was wondering if anyone has had problems with xylene fumes using Sakura VIP Tissue processor. Ours is currently sitting on a bench in a room with a snorkle right above is and the smell is still really bad and is causing headaches for people who try to work in that room. Does anyone have any suggestions? Thank you, Julie Novartis Institues for Biomedical Research Cambridge, MA" It sounds as though it is time to replace the carbon filter on the VIP processor. This should be done on a regular basis depending on usage. Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm From ACHSTETT <@t> afip.osd.mil Fri Jul 9 13:53:10 2004 From: ACHSTETT <@t> afip.osd.mil (Achstetter, Virginia A.) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] (no subject) Message-ID: <9C631520464F9E4BA5B111450F2A0AAD0BC9FB@lewis.afip.osd.mil> Our department wants to purchase a tissue arrayer from Beecher Instruments. All attempts to contact them has failed. Email address is no longer valid. Fax goes unanswered as well as phone and snail mail. Does anyone know where we could purchase this equipment or how to get in touch with Beecher Instruments? Ginny Achstetter HT (ASCP) Armed Forces Institute of Pathology Soft Tissue Pathology 6825 16th St. NW Bldg 54 Rm. 3118 Washington, DC 20306 202-782-2813 From mtodd <@t> medscape.com Fri Jul 9 14:09:24 2004 From: mtodd <@t> medscape.com (Michael Todd) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] Mohs Histotech; Xylene substitute Message-ID: I am opening a Mohs laboratory in Northern Virginia, just outside Washington DC, and need some help. 1. I need a Histotech, either PT or FT. Beginning September, 2004. 2. I have been following the conversation about xylene substitutes - and from what I gather, they can do a good job. I am wondering if anyone has used "Slide Brite." What the sales rep tells me sounds great, but can anyone speak from their own experience? Thanks Mike Todd Skin Cancer Center of Northern Virginia Sent by Medscape Mail: Free Portable E-mail for Professionals on the Move http://www.medscape.com From Ben.Shelkowsky <@t> chomp.org Fri Jul 9 14:21:01 2004 From: Ben.Shelkowsky <@t> chomp.org (Shelkowsky, Ben) Date: Fri Sep 16 15:23:42 2005 Subject: [Histonet] DEZENKERIZE USING SAKURA DRS STAINER Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D612F@exchsrvr.chomp.org> I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From funderwood <@t> mcohio.org Fri Jul 9 14:36:53 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:42 2005 Subject: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER Message-ID: Set up a reagent station with idodine, 5 minutes shoud do. From there just go through your rehydrating alcohols, tap water wash, and continue your staining procedure. Fred >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rockbeki <@t> ufl.edu Fri Jul 9 16:06:19 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] (no subject) Message-ID: <622625536.1089407179226.JavaMail.osg@spnode33> Which system? On Wed Jul 07 04:35:02 EDT 2004, JAnorthernexp@cs.com wrote: > Hi there, > Does anyone out there use this system and what is your procedure > for cutting (micron thickness), drying time and temperature for > slides or do you air dry using charge slides. And do you use a > decloaker for deparaffinizing and antigen retrieval. thanks, > > Annette Hart, HT (ASCP) > Wyoming Valley Health Care System > Wilkes-Barre, PA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From Laurie.Pereira <@t> sdcounty.ca.gov Fri Jul 9 16:28:24 2004 From: Laurie.Pereira <@t> sdcounty.ca.gov (Pereira, Laurie ) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] re: 266mp manual Message-ID: I have a manual for the fisher tissue processor 166/266mp. The manual is approximately 59 pages long and I could make a copy. Just let me know where to send it to. Laurie Pereira SDCADDL (Office of the County Veterinarian) laurie.pereira@sdcounty.ca.gov From amoklebu <@t> seattlecca.org Fri Jul 9 16:27:04 2004 From: amoklebu <@t> seattlecca.org (Moklebust, Amanda C) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] B-Plus substitute for B5 Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF306@wala01.seattlecca.org> It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From amarusk1 <@t> FAIRVIEW.ORG Fri Jul 9 17:41:27 2004 From: amarusk1 <@t> FAIRVIEW.ORG (ANN MARUSKA) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] unsubscribe Message-ID: please unsubscribe The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> From Jason.Wiese <@t> med.va.gov Fri Jul 9 17:59:14 2004 From: Jason.Wiese <@t> med.va.gov (Wiese, Jason VHAROS) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] B-Plus substitute for B5 Message-ID: I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Fri Jul 9 19:19:04 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] B-Plus substitute for B5 Message-ID: We use "Prefer" and/or "Z-Fix" as our B-5 replacements. Both are manufactured by Anatech. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@med.va.gov] Sent: Friday, July 09, 2004 3:59 PM To: 'Moklebust, Amanda C'; 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Fri Jul 9 20:14:54 2004 From: tahseen <@t> brain.net.pk (Muhammad Tahseen) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Room Temperature References: <002201c464f4$0f7d8a60$5a0cd2aa@unl.edu.ar> Message-ID: <008001c4661b$50e8ca00$972bfea9@User> 25c ----- Original Message ----- From: Lab Histologia To: Sent: Thursday, July 08, 2004 7:01 PM Subject: [Histonet] Room Temperature > Hello, everyone, > > I need know that range of temperature defines you as room temperature. > > Thanks > > > Hugo > > ------------------------------------------------------------------- > > Dr. Hugo H. Ortega (DMV, PhD) > > Departament of Cellular Biology > > Faculty of Veterinary Sciences > > Universidad Nacional del Litoral > > R.P. Kreder 2805 - Esperanza (3080) > > Santa Fe - ARGENTINA > > Tel. (54)3496-420639 > > Fax. (54)3496-426304 > > http://fcv.unl.edu.ar/histolog/ > > http://fcv.unl.edu.ar/bioterio/ > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> sbcglobal.net Sat Jul 10 04:57:51 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:43 2005 Subject: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER References: Message-ID: <003c01c46664$5d32f580$9f27d445@domainnotset.invalid> Reminder to everyone who is dezenkerizing (lugolizing) on the stainers - when you get ready to dump this iodine station, it cannot go down the sink. It now contains mercuric salts, so must be collected as mercury waste. (As should the next couple of containers of reagents, carry-over on the slides.) Ways to get around this: 1. Dezenkerize (lugolize) by hand, then add the slide to the stainer. 2. Switch over to a zinc fixative. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Fred Underwood" To: Sent: Friday, July 09, 2004 3:36 PM Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER > Set up a reagent station with idodine, 5 minutes shoud do. From there > just go through your rehydrating alcohols, tap water wash, and continue > your staining procedure. > > Fred > > >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> > I would like to dezenkerize my slides from mercury-fixed tissues on the > SAKURA automated stainer in a program I can internalize on the menu. Is > anyone out there already doing this and can you send me your procedure? > > We would appreciate any help on this matter. > > Ben Shelkowsky > CHOMP > Monterey, CA. 93950 > ben.shelkowsky@chomp.org > Confidentiality Notice: > This is a transmission from Community Hospital of the Monterey > Peninsula. This message and any attached documents may be confidential > and contain information protected by state and federal medical privacy > statutes. They are intended only for the use of the addressee. If you > are not the intended recipient, any disclosure, copying, or distribution > of this information is strictly prohibited. If you received this > transmission in error, please accept our apologies and notify the > sender. > > Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sat Jul 10 05:08:34 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Room Temperature References: <002201c464f4$0f7d8a60$5a0cd2aa@unl.edu.ar> <008001c4661b$50e8ca00$972bfea9@User> Message-ID: <004501c46665$dcc2cae0$9f27d445@domainnotset.invalid> Take the temperature of YOUR room, and use that. Muhammad said 25 degrees C. 25 degrees C = 77 degrees F That would be too hot in our lab in Michigan, which is air conditioned in the spring/summer/fall, and never too hot when the furnace is on in the winter. Plus, with the increased air ventilation/flow, it always seems chillier. 22 degrees C = 71.6 degrees F. 20 degrees C = 68 degrees F. That's more the range in our anatomic pathology labs. Of course, one year they move the student lab into a room that the heat didn't work very well. We did have a large picture window, but there was a sheet of ice on it all winter long. Our average that winter was: 15 degrees C = 59 degrees F Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Muhammad Tahseen" To: "Lab Histologia" ; Sent: Friday, July 09, 2004 9:14 PM Subject: Re: [Histonet] Room Temperature > 25c > ----- Original Message ----- > From: Lab Histologia > To: > Sent: Thursday, July 08, 2004 7:01 PM > Subject: [Histonet] Room Temperature > > > > Hello, everyone, > > > > I need know that range of temperature defines you as room temperature. > > > > Thanks > > > > > > Hugo > > > > ------------------------------------------------------------------- > > > > Dr. Hugo H. Ortega (DMV, PhD) > > > > Departament of Cellular Biology > > > > Faculty of Veterinary Sciences > > > > Universidad Nacional del Litoral > > > > R.P. Kreder 2805 - Esperanza (3080) > > > > Santa Fe - ARGENTINA > > > > Tel. (54)3496-420639 > > > > Fax. (54)3496-426304 > > > > http://fcv.unl.edu.ar/histolog/ > > > > http://fcv.unl.edu.ar/bioterio/ > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BWinters <@t> NCH.ORG Sat Jul 10 07:37:57 2004 From: BWinters <@t> NCH.ORG (Winters, Bert) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] PROSTATE BIOSIES Message-ID: <270614B321ACB44D8C1D91F4F921FDC362776D@NCH01EX02.nch.org> I am looking for any suggestions when it comes to processing prostate biopsies. The pathologist have going back and forth with processing these biopsies using a typical biopsy program on the tissue processors and using a routine process which is used for processing most tissue specimens. Everyone I have talked with about this processes all prostate biopsies using a biopsy program, about 5-6 hours in the tissue processor. Any processing or staining tips anyone could give me about prostate biopsies would be greatly appreciated. Bert From pengbw <@t> sjtu.edu.cn Sun Jul 11 00:17:12 2004 From: pengbw <@t> sjtu.edu.cn (Baowei Peng) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Tunel assay Message-ID: <20040711051712.050EA10F887B@sjtu.edu.cn> Hi All, Does anyone here can share me a practical protocol of Tunel assay on frozen sections? Baowei Peng Shanghai Jiaotong University Shanghai, 2003 China From sulekhababy <@t> yahoo.co.uk Sun Jul 11 06:39:19 2004 From: sulekhababy <@t> yahoo.co.uk (=?iso-8859-1?q?Sulekha=20Ravi?=) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Fading of Hematoxylin Message-ID: <20040711113919.83497.qmail@web25003.mail.ukl.yahoo.com> Hi Please help me in overcoming this problem. Hematoxylin in my H&E stained slides are fading in 1 month. I am using 'Gurr' make Hematoxylin powder to make Harrys' Hematoxylin. Fresh stain is prepared every 3 months. Kindly advise what to do. Sulekha --------------------------------- ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself From Barry.R.Rittman <@t> uth.tmc.edu Sun Jul 11 08:26:26 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Fading of Hematoxylin Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0635AE6@UTHEVS3.mail.uthouston.edu> Most permanent mounting media tend to cause some fading of hematoxylin probably due to its continued oxidation. This is however, generally not as rapid as the time period that you indicate. We have seen some problems in the past when batches of clearing agent (xylene, which is really a mixture of xylenes) had some contaminants and this problem was eliminated using better quality xylene. One medium that retained hematoxylin was Euparal vert from George Gurr Co. This contained a copper salt that in effect produced a copper hematoxylin, much more resistant to fading. I wonder whether converting the hematoxylin to a copper hematoxylin or an iron hematoxylin might solve this problem or perhaps incorporating a reducing agent in the mountant. Eosin fading is another problem. If you have old faded slides can look at them using fluorescence microscopy for the eosin. Would be interesting to have a Histonet discussion of this. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sulekha Ravi Sent: Sun 7/11/2004 6:39 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Fading of Hematoxylin Hi Please help me in overcoming this problem. Hematoxylin in my H&E stained slides are fading in 1 month. I am using 'Gurr' make Hematoxylin powder to make Harrys' Hematoxylin. Fresh stain is prepared every 3 months. Kindly advise what to do. Sulekha --------------------------------- ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sun Jul 11 12:30:46 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] re fading of Hx Message-ID: <001701c4676c$cd5a1ba0$66cc9a51@home> Do you use xylene to mount from? If so, do you use the "low in sulphur" type? I remember someone having a similar problem ( more pronounced in the eosin, tho) when they didn't use that particular type. Their problem was solved when they substituted the low sulphur xylene --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 From kerrie.thomson.kt <@t> bayer-ag.de Sun Jul 11 18:04:48 2004 From: kerrie.thomson.kt <@t> bayer-ag.de (kerrie.thomson.kt@bayer-ag.de) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Unsubscribe please Message-ID: histonet-bounces@lists.utsouth western.edu To: histonet@lists.utsouthwestern.edu cc: 05/07/04 03:05 AM Subject: Histonet Digest, Vol 8, Issue 8 Please respond to histonet Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pneumocystis by polarization (RSRICHMOND@aol.com) 2. RE: CAP and Computer Training (Valerie Biendara) ---------------------------------------------------------------------- Message: 1 Date: Sat, 3 Jul 2004 14:18:18 EDT From: RSRICHMOND@aol.com Subject: [Histonet] pneumocystis by polarization To: histonet@lists.utsouthwestern.edu Message-ID: <98.edae86b.2e18526a@aol.com> Content-Type: text/plain; charset="US-ASCII" Sue O'Brien, Histology Supervisor, at Burdette Tomlin Memorial Hospital, Cape May Court House, New Jersey, asks: >>I was wondering if anyone else had tried Dr. Teisa An's method (published in the March 2004 issue of Arch. Pathol. Lab. Med.)? It utilizes pepsin, and then the PCP can be visualized via polarization. For those that have tried it, what do you think? It is very easy to do the procedure, but the results seem a little different (for someone used to looking at silver stained material).<< The article is "The use of polarization microscopy in the diagnosis of pneumocystis pneumonia" by Teisa An MD and Pam Tabaczka BS(MT) in the pathology department at Harper University Hospital in Detroit MI (Dr. An's e-mail is tan at dmc.org.), in Arch Pathol Lab Med 2004;128:163-4. They write "When tissue sections that had been treated with pepsin and stained with hematoxylin only were examined under polarized light, the cyst forms of P carinii were observed as being clearly birefringent and were as numerous as when a Grocott methenamine silver-stained slide was examined." This article is a good example of why I get the Archives because the College of American Pathologists sends it to me, but usually let it accumulate on top of the previous month's number. The authors report exactly one case, without even noting whether a pneumocystis control slide was positive, how they applied the pepsin, or what hematoxylin they used. They offer no relevant references, and no explanation of how the method might work. They don't mention that the present name of the human pathogen is Pneumocystis jiroveci. If the Archives is actually a refereed journal, then taunts about the umpire's seeing-eye dog are definitely in order. Still, it couldn't hurt to try the method, but it's hardly ready for prime time. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC ------------------------------ Message: 2 Date: Fri, 2 Jul 2004 20:31:18 -0500 From: "Valerie Biendara" Subject: RE: [Histonet] CAP and Computer Training To: Message-ID: <20040703013142.KIWX14781.fe6@Valerie> Content-Type: text/plain; charset="US-ASCII" See CAP Checklist, Laboratory General GEN.44500 Phase II N/A YES NO Is there documentation that all users of the computer system receive adequate training initially, after system modification, and after installation of a new system? COMMENTARY: The people who interact with the computer system must initially be taught how to use a new system and must be trained on modifications to an existing system. There must be documentation of these training activities. Victor, I'm preparing for my CAP for the Fall and I don't see anything mentioned about computer training. If there is something, please let me know. I must be missing it. We have been going round and round over this. Someone has said that CAP requires semi-annual refresher training on the computer system, but we can't find any documentation. Help!! Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 8 ************************************** From AnthonyH <@t> chw.edu.au Sun Jul 11 18:14:07 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Fading of Hematoxylin Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E208@simba.kids> Dear Sulekha, What coverslip mountant are you using? Some will fade slides over time. Regards, Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Sulekha Ravi [mailto:sulekhababy@yahoo.co.uk] Sent: Sunday, 11 July 2004 9:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fading of Hematoxylin Hi Please help me in overcoming this problem. Hematoxylin in my H&E stained slides are fading in 1 month. I am using 'Gurr' make Hematoxylin powder to make Harrys' Hematoxylin. Fresh stain is prepared every 3 months. Kindly advise what to do. Sulekha --------------------------------- ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From CrochiereSteve <@t> aol.com Sun Jul 11 19:55:58 2004 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] am I still on the lists? Message-ID: <21.40ace3c2.2e233b9e@aol.com> From CrochiereSteve <@t> aol.com Sun Jul 11 19:55:21 2004 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] why no mail? Message-ID: <1ab.267eb3af.2e233b79@aol.com> I haven't received the usual number of histonet postings during my vaction last week. Has the syatem been on holiday also? Just curious. From Joachim.Velden <@t> evotec-neurosciences.com Mon Jul 12 04:48:38 2004 From: Joachim.Velden <@t> evotec-neurosciences.com (Joachim.Velden@evotec-neurosciences.com) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Alkaline NaCl saturated Congo Red Procedure (Puchtler et al. 1962) Message-ID: Dear Histonet Members, could someone please explain to me the purpose/effect of alkalinization by adding 1 ml of 250 mM NaOH to 100 ml of the staining solution used in Puchtler's alkaline, NaCl-saturated Congo Red staining method? Accidentally, I once forgot to add the NaOH, but the staining results did not differ from those that we usually obtain when we stick to the alkalinizing protocol. Can one omit the alkalinization without doing any harm to the method's nice sensitivity and specificity towards amyloid and/or reproducibility of staining results? What is the alkalinization intended to be good for? This question is aimed at some practical consequences: Omitting the alkalinization step would (i) save time, (ii) spare using corrosive agent (NaOH), (iii) leave the staining solution more stable (than for less than one day with NaOH added) and thus (iv) save staining reagents (particularly expensive ethanol) and congo red dye. Thank you for your expert opinion. Best regards, Joachim ----------------------------------------------------------------------------------------- Dr. Joachim Velden Evotec Neurosciences GmbH Schnackenburgallee 114 D-22525 Hamburg Germany Phone +49-40-56081-394 Fax +49-40-56081-222 joachim.velden@evotec-neurosciences.com http://www.evotec-neurosciences.com From fmonson <@t> wcupa.edu Mon Jul 12 08:00:13 2004 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Fading of Hematoxylin Message-ID: Fading of Hematoxylin is almost always caused by a mountant whose pH has become acidic. Remember how you differentiate the progressive hematoxylin dyes. You must check the pH of your mountant. A method by which one may 'keep' the mountant at neutral pH is to load the storage container with a reasonable amount of marble chips. This is an 'old' method, and I have never had to use it since I prepare my own mountant from dewaxed Gum Damar. Of course, I can do that, because I have no volume, and no bean counters in my 1-man organization. BTW, it is likely that if the mountant you are using is from a new or recently opened bottle of mountant, the entire bottle was contaminated to begin with. Cheers, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SSS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Sulekha Ravi [mailto:sulekhababy@yahoo.co.uk] Sent: Sunday, July 11, 2004 7:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fading of Hematoxylin Hi Please help me in overcoming this problem. Hematoxylin in my H&E stained slides are fading in 1 month. I am using 'Gurr' make Hematoxylin powder to make Harrys' Hematoxylin. Fresh stain is prepared every 3 months. Kindly advise what to do. Sulekha --------------------------------- ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> polysciences.com Mon Jul 12 07:59:00 2004 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Room Temperature In-Reply-To: <008001c4661b$50e8ca00$972bfea9@User> Message-ID: <001501c4680f$ffeb37e0$4000a8c0@PMARCUM2K> Hi, Depending on where you are and the addition of air conditioning or heating the range is generally between 18C and 22C. We use an average when doing packaging of 20C for most room temperature storage. Unfortunately it is very difficult to give an exact room temperature as the climate in different areas can be great and the availability of cooling or heating is not constant. Storage of most reagents at room temperature above the given range can cause a problem and one must also consider humidity when looking at how reagents and chemicals are kept. Humidity can have a much more disastrous effect than temperature for some reagents and dyes. Thanks, Pam Marcum? > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Muhammad > Tahseen > Sent: Friday, July 09, 2004 9:15 PM > To: Lab Histologia; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Room Temperature > > > 25c > ----- Original Message ----- > From: Lab Histologia > To: > Sent: Thursday, July 08, 2004 7:01 PM > Subject: [Histonet] Room Temperature > > > > Hello, everyone, > > > > I need know that range of temperature defines you as room temperature. > > > > Thanks > > > > > > Hugo > > > > ------------------------------------------------------------------- > > > > Dr. Hugo H. Ortega (DMV, PhD) > > > > Departament of Cellular Biology > > > > Faculty of Veterinary Sciences > > > > Universidad Nacional del Litoral > > > > R.P. Kreder 2805 - Esperanza (3080) > > > > Santa Fe - ARGENTINA > > > > Tel. (54)3496-420639 > > > > Fax. (54)3496-426304 > > > > http://fcv.unl.edu.ar/histolog/ > > > > http://fcv.unl.edu.ar/bioterio/ > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cgfields <@t> lexhealth.org Mon Jul 12 08:24:29 2004 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] PROSTATE BIOSIES Message-ID: Check website http://www.palpath.com/ (then click on Agar embedding & photos) This will show you how to pre-embed your prostate bx specimens in agar insuring the correct orientation. Carole Fields Lexington Med Ctn W.Columbia, SC -----Original Message----- From: Winters, Bert [mailto:BWinters@NCH.ORG] Sent: Saturday, July 10, 2004 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PROSTATE BIOSIES I am looking for any suggestions when it comes to processing prostate biopsies. The pathologist have going back and forth with processing these biopsies using a typical biopsy program on the tissue processors and using a routine process which is used for processing most tissue specimens. Everyone I have talked with about this processes all prostate biopsies using a biopsy program, about 5-6 hours in the tissue processor. Any processing or staining tips anyone could give me about prostate biopsies would be greatly appreciated. Bert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From rentonlf <@t> bru.wits.ac.za Mon Jul 12 08:03:57 2004 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] test message Message-ID: <1089637437.8aa10f20rentonlf@bru.wits.ac.za> checking to see if I'll be bounced again Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From rentonlf <@t> bru.wits.ac.za Mon Jul 12 08:44:17 2004 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] adhesive tape & MMA Sections Message-ID: <1089639857.8cf75d00rentonlf@bru.wits.ac.za> OK, this is the last time I'm posting this, so if this is version #54, please bear with me, as it seems some sort of gremlin crept into the system. The question is: How do I treat MMA sections that are stuck onto common office type sticky tape? Do I: a) Stain first & stick onto a slide later -if so what type of adhesive will work best? b)Stick down first & remove sticky tape ( what solvent to try I'm scared I'll lose the section altogether) c)Stain, stick down & ignore the sticky tape & mount as usual. Any & all help will be appreciated (but please don't ask me to consider the Instrumedics system - its not appropriate for this application) Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From gcallis <@t> montana.edu Mon Jul 12 10:01:46 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] RE: Fading of Hematoxylin In-Reply-To: <566FB0B522443D43AF02D2ADBE35A6F0635AE6@UTHEVS3.mail.uthous ton.edu> Message-ID: <3.0.6.32.20040712090146.00c14690@gemini.msu.montana.edu> Barry gave good advice, look for a permanent mounting media that contains antioxidative or a good reducing agent. Many mounting medias are available. Also when doing your hematoxylin staining, be sure you incorporate good water rinses between steps to have the correct pH for staining. 1 minute of running tap water rinse between each step should do the job nicely. A 70% alcohol rinse before eosin (or use the same concentration of alcohol of the eosin solution) removes any cations that can interfere with the eosin staining. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) At 08:26 AM 7/11/2004 -0500, you wrote: >content-class: urn:content-classes:message >Content-Type: text/plain; charset="utf-8" > >Most permanent mounting media tend to cause some fading of hematoxylin probably due to its continued oxidation. This is however, generally not as rapid as the time period that you indicate. >We have seen some problems in the past when batches of clearing agent (xylene, which is really a mixture of xylenes) had some contaminants and this problem was eliminated using better quality xylene. >One medium that retained hematoxylin was Euparal vert from George Gurr Co. This contained a copper salt that in effect produced a copper hematoxylin, much more resistant to fading. I wonder whether converting the hematoxylin to a copper hematoxylin or an iron hematoxylin might solve this problem or perhaps incorporating a reducing agent in the mountant. Eosin fading is another problem. If you have old faded slides can look at them using fluorescence microscopy for the eosin. >Would be interesting to have a Histonet discussion of this. >Barry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sulekha Ravi >Sent: Sun 7/11/2004 6:39 AM >To: histonet@lists.utsouthwestern.edu >Cc: >Subject: [Histonet] Fading of Hematoxylin > > > > Hi > Please help me in overcoming this problem. Hematoxylin in my H&E stained slides are fading in 1 month. I am using 'Gurr' make Hematoxylin powder to make Harrys' Hematoxylin. Fresh stain is prepared every 3 months. Kindly advise what to do. > Sulekha > > > --------------------------------- > ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Pat.Willis <@t> mail.bhrv.nwest.nhs.uk Mon Jul 12 10:03:00 2004 From: Pat.Willis <@t> mail.bhrv.nwest.nhs.uk (Pat Willis) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Control block for leprosy[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D1BD7F6@BHRV_NT_11> Is there anybody out there with a known supply of leprosy control blocks? Preferably UK based. We'd appreciate it if you could contact us with details. Thanks in advance, Pat From ccrowder <@t> mail.vetmed.lsu.edu Mon Jul 12 10:07:54 2004 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Dezenkerizing slides Message-ID: Ben - Years ago, when Zenker's was used by everyone, we used to put alcoholic iodine as the first station on the processor (after formalin). Then we added a sodium thiosulfate rinse to the stain routine. That took an extra minute or 2. You might try that. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From cormier <@t> MIT.EDU Mon Jul 12 10:25:26 2004 From: cormier <@t> MIT.EDU (Kathleen Cormier) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Hepatocytes trichrome Message-ID: <5.2.1.1.2.20040712112057.00ae9f10@hesiod> Hi all! I have a question for you liver people out there.. Is there a trichrome stain out there they people use that will stain collagen as usual and NOT stain hepatocytes red or so red like the muscle in the trichrome? I (yet again) have a researcher that would like a trichrome on livers but would like to see the hepatocytes less red or ideally a different color. This is on FFPE tissue. Thanks! Kathy Cormier DCM - MIT From bryand <@t> netbistro.com Mon Jul 12 11:00:13 2004 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Alkaline NaCl saturated Congo Red Procedure (Puchtler et al. 1962) In-Reply-To: References: Message-ID: <40F2B58D.4000805@netbistro.com> Congo red (and similar dyes) stains amyloid by hydrogen bonding. It can also combine with other tissue components ionically since it is an acid dye. Using an alkaline solvent reduces ionic bonding but permits hydrogen bonding to go ahead. The amyloid will stain regardless of whether you add alkali or not, but eosinophilic (congoredophilic?) components may well stain pink and cause confusion since they will be the same colour as the amyloid (but will not display green birefringence). With the alkali you are assured that only amyloid (and possibly eosinophils and paneth cell granules) will be pink. Incidentally, the solvent is ethanolic. This is to reduce the polarity of the solution and thereby inhibit ionic bonding while promoting hydrogen bonding. The ethanolic solvent in conjunction with the alkali is what causes the specificity of the technique. Changing either would affect the results to some degree. If you left out the alkali you would likely notice a difference in the long term. Bryan Llewellyn Joachim.Velden@evotec-neurosciences.com wrote: > Dear Histonet Members, > > could someone please explain to me the purpose/effect of alkalinization by > adding 1 ml of 250 mM NaOH to 100 ml of the staining solution used in > Puchtler's alkaline, NaCl-saturated Congo Red staining method? > Accidentally, I once forgot to add the NaOH, but the staining results did > not differ from those that we usually obtain when we stick to the > alkalinizing protocol. Can one omit the alkalinization without doing any > harm to the method's nice sensitivity and specificity towards amyloid > and/or reproducibility of staining results? What is the alkalinization > intended to be good for? > This question is aimed at some practical consequences: Omitting the > alkalinization step would (i) save time, (ii) spare using corrosive agent > (NaOH), (iii) leave the staining solution more stable (than for less than > one day with NaOH added) and thus (iv) save staining reagents > (particularly expensive ethanol) and congo red dye. > > Thank you for your expert opinion. > > Best regards, > > Joachim > > > > ----------------------------------------------------------------------------------------- > Dr. Joachim Velden > Evotec Neurosciences GmbH > Schnackenburgallee 114 > D-22525 Hamburg > Germany > > Phone +49-40-56081-394 Fax +49-40-56081-222 > joachim.velden@evotec-neurosciences.com > http://www.evotec-neurosciences.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From histomjans <@t> yahoo.com Mon Jul 12 11:32:13 2004 From: histomjans <@t> yahoo.com (Melissa Jans) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] cryostat purchase Message-ID: <20040712163213.88159.qmail@web50303.mail.yahoo.com> Vendors welcome to reply.... We are looking to purchase 2 cryostats. I have been given a very small window of time in which I must make a decision and purchase. We are a large laboratory with 3 cryostats right now. Given current CAP regulations, each cryostat must defrosted, unassembled, decontaminated, and reassembled each week. This has caused our cryostats to become a little "loose." I will not mention our current vendor, but will merely say when these cryostats were designed, I do not believe they were ever intended to be taken apart and put back together so frequently. I am hoping some fellow histonetters will have some vendors and models of recently purchased cryostats. Important features for our lab include: ease of decontamination - unassembly/assembly (self-decontamination would be great), ease of use (we are a teaching hospital and do not want anything with too many buttons!), quality of sections, movable specimen head, large chamber size (enough room for at least 6-10 freezing stations). I could go on and on.... Please send any suggestions my way. I have been looking at the archives.....with minimal success. Thanks! Melissa Jans University of Iowa Hospitals and Clinics __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Mon Jul 12 13:31:13 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Dezenkerizing slides In-Reply-To: Message-ID: <3.0.6.32.20040712123113.00bfc760@gemini.msu.montana.edu> Hi Cheryl and all, This answer has come up twice, but what is the percentage of iodine in the 70%alcohol IF one can't locate it in a book, etc? >Ben - Years ago, when Zenker's was used by everyone, we used to put >alcoholic iodine as the first station on the processor (after formalin). >Then we added a sodium thiosulfate rinse to the stain routine. That took an >extra minute or 2. You might try that. Cheryl > >Cheryl Crowder, BA, HTL(ASCP) >Chief Technologist >Anatomic Pathology >Department of Pathobiological Sciences >School of Veterinary Medicine >Louisiana State University >Skip Bertman Drive >Baton Rouge, LA 70803 > >225-578-9734 >FAX: 225-578-9720 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From joseph-galbraith <@t> uiowa.edu Mon Jul 12 13:43:03 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Hepatocytes trichrome Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008602@uihc-mail1.uihc.uiowa.edu> Kathy: We use a modified trichrome called a Klatskin stain (after Dr Klatskin, a famous liver pathologist who developed the stain). The collagen is blue (analine blue), the liver cells are red (Ponceau Red) but stain variably dark depending on the 'granularity' of the cells. Nuclei are blue/purple of course (Gills). Not certain if this would be of help since you are concerned about red liver cells against red muscle but the Ponceau does tend to stain each with a slightly different shade. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kathleen Cormier Sent: Monday, July 12, 2004 10:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Hepatocytes trichrome Hi all! I have a question for you liver people out there.. Is there a trichrome stain out there they people use that will stain collagen as usual and NOT stain hepatocytes red or so red like the muscle in the trichrome? I (yet again) have a researcher that would like a trichrome on livers but would like to see the hepatocytes less red or ideally a different color. This is on FFPE tissue. Thanks! Kathy Cormier DCM - MIT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Jul 12 13:53:19 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Re: Hepatocytes trichrome In-Reply-To: <5.2.1.1.2.20040712112057.00ae9f10@hesiod> Message-ID: <3.0.6.32.20040712125319.00bfc760@gemini.msu.montana.edu> It is important to remember Massons trichrome stains ALL connective tissue components, collagen, reticulum fibers and basement membranes. If you want collagen only, you should do Van Giesons stain. You did not say which trichrome method you are using, the classic Massons Trichrome? IN Massons trichrome, the Biebrich scarlet - acid fuchsin step can be controlled by 1. Less staining time in Biebrich scarlet/AF solution, if your method says 5 min, try 1 min. 2. Phoshptungstic acid/phosphomolybdic acid step in controlled with microscope until stain is differentiated out of cells. It may take longer than time stated in your protocol for PTAH/PMA ( his solution shoule be fresh each time). OR one can simple eliminate the biebrich scarlet/acid fushsin, just do aniline blue alone with Weigerts iron hematoxylin. If you don't need to see muscle or red blood cells, just take away the red! This leaves connective tissues blue with darker staining nuclei. We used the Richard Allan trichrome kit last week for the first time and had the kind of staining you describe with nice staining on lung but without overstaining of cells other than muscle. Red Blood cells were spectacularly red, however. It could be the timing of reagents developed for their kit that makes it work nicely. We did let sections sit in Bouins overnight at RT. AND we do NOT use a microwave for Bouins step, everything was done the old fashioned long way. It you microwave Bouins, the postfixation may not be adequate (this came from Jerry Fredenburgh - RA guru also with the staining kits!) and this can affect your staining outcome. At 11:25 AM 7/12/2004 -0400, you wrote: >Hi all! > >I have a question for you liver people out there.. Is there a trichrome >stain out there they people use that will stain collagen as usual and NOT >stain hepatocytes red or so red like the muscle in the trichrome? I (yet >again) have a researcher that would like a trichrome on livers but would >like to see the hepatocytes less red or ideally a different color. This is >on FFPE tissue. Thanks! > >Kathy Cormier >DCM - MIT > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From cgfields <@t> lexhealth.org Mon Jul 12 13:57:05 2004 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:23:43 2005 Subject: FW: [Histonet] Dezenkerizing slides Message-ID: oops..I forgot to send it to the HistoNet. CF -----Original Message----- From: Carole Fields Sent: Monday, July 12, 2004 2:55 PM To: 'Gayle Callis' Subject: RE: [Histonet] Dezenkerizing slides It is in Ann Preese, us old-timers remember. 0.5% Alc-Iodine. It is the only place I could find it. We use it every day. 5 gms Iodine 1000ml 80% alcoh Dissolve Iodine in 10 to 20 ml of the 80% alcoh. When dissolved add the remainder of the alcohol. We run our slides down on the stainer to 95% alc then they go into Alc-Iodine for 6-8 min. then wash and finish staining H&E. The Iodine washes out and you are done. You do not need the hypo. Carole Fields Lex Med Ctn W.Columbia, SC -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Monday, July 12, 2004 2:31 PM To: Cheryl Crowder; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dezenkerizing slides Hi Cheryl and all, This answer has come up twice, but what is the percentage of iodine in the 70%alcohol IF one can't locate it in a book, etc? >Ben - Years ago, when Zenker's was used by everyone, we used to put >alcoholic iodine as the first station on the processor (after formalin). >Then we added a sodium thiosulfate rinse to the stain routine. That took an >extra minute or 2. You might try that. Cheryl > >Cheryl Crowder, BA, HTL(ASCP) >Chief Technologist >Anatomic Pathology >Department of Pathobiological Sciences >School of Veterinary Medicine >Louisiana State University >Skip Bertman Drive >Baton Rouge, LA 70803 > >225-578-9734 >FAX: 225-578-9720 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From cwscouten <@t> myneurolab.com Mon Jul 12 13:58:30 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] cryostat purchase Message-ID: The Vibrotome Model 7500 Cryostat is self decontaminating. Just push a button. See the link below. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Jans Sent: Monday, July 12, 2004 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat purchase Vendors welcome to reply.... We are looking to purchase 2 cryostats. I have been given a very small window of time in which I must make a decision and purchase. We are a large laboratory with 3 cryostats right now. Given current CAP regulations, each cryostat must defrosted, unassembled, decontaminated, and reassembled each week. This has caused our cryostats to become a little "loose." I will not mention our current vendor, but will merely say when these cryostats were designed, I do not believe they were ever intended to be taken apart and put back together so frequently. I am hoping some fellow histonetters will have some vendors and models of recently purchased cryostats. Important features for our lab include: ease of decontamination - unassembly/assembly (self-decontamination would be great), ease of use (we are a teaching hospital and do not want anything with too many buttons!), quality of sections, movable specimen head, large chamber size (enough room for at least 6-10 freezing stations). I could go on and on.... Please send any suggestions my way. I have been looking at the archives.....with minimal success. Thanks! Melissa Jans University of Iowa Hospitals and Clinics __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Mon Jul 12 14:27:02 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] nair, exoskeletons and IHC Message-ID: Does anyone know if softening up the exoskeletons on ants with nair would interfere with IHC or insitu? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From rgrow <@t> bmnet.com Mon Jul 12 14:50:59 2004 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Re:cryostat purchase Message-ID: Melissa, You may consider the Thermo-Electron Cryotome E. It has made a considerable difference in our frozen section quality, turn-around time, and performance. It has an automated decontamination process that fumigates the chamber with formalin once a week, nightly, or immediately. There is no need to disassemble, as the formalin fumes infiltrate all internal chamber orifices. Then microtome is housed outside the frozen chamber. (No ice buildup in the moving parts) We wipe out debris daily with ethanol. We were recently inspected by CAP and passed using this procedure. There is a generous sized container that captures melt-off fluids for easy disposal located on the front of the cryostat. The large chamber can accommodate multiple blocks at a time. All controls are on the outside (no more frozen fingers). Automatically retract/advance, macro or micro, with a push of a button. There are a few too many push buttons on the control panel, but I solved that problem by taping a tongue depressor over those that could cause problems. Hope this helps. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 17 Date: Mon, 12 Jul 2004 09:32:13 -0700 (PDT) From: Melissa Jans Subject: [Histonet] cryostat purchase To: histonet@lists.utsouthwestern.edu Message-ID: <20040712163213.88159.qmail@web50303.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Vendors welcome to reply.... We are looking to purchase 2 cryostats. I have been given a very small window of time in which I must make a decision and purchase. We are a large laboratory with 3 cryostats right now. Given current CAP regulations, each cryostat must defrosted, unassembled, decontaminated, and reassembled each week. This has caused our cryostats to become a little "loose." I will not mention our current vendor, but will merely say when these cryostats were designed, I do not believe they were ever intended to be taken apart and put back together so frequently. I am hoping some fellow histonetters will have some vendors and models of recently purchased cryostats. Important features for our lab include: ease of decontamination - unassembly/assembly (self-decontamination would be great), ease of use (we are a teaching hospital and do not want anything with too many buttons!), quality of sections, movable specimen head, large chamber size (enough room for at least 6-10 freezing stations). I could go on and on.... Please send any suggestions my way. I have been looking at the archives.....with minimal success. Thanks! Melissa Jans University of Iowa Hospitals and Clinics _______________________________________________________________ This E-mail contains PRIVILEGED AND CONFIDENTIAL INFORMATION intended only for the use of the Individual(s) named above. If you are not the intended recipient of this E-mail, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this E-mail is strictly prohibited. If you have received this E-mail in error, please immediately notify us at 865-977-5652 or notify us by E-mail at helpdesk@bmnet.com From mtodd <@t> medscape.com Mon Jul 12 20:46:05 2004 From: mtodd <@t> medscape.com (Michael Todd) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Microm 505EVP cryostat Message-ID: <5D3EA691799C8B549B231880B237F710@mtodd.medscape.com> Has anyone used the Microm 505 EVP (with Peltier cooling and vacutome)? If so, how is it? Thanks Mike Todd Sent by Medscape Mail: Free Portable E-mail for Professionals on the Move http://www.medscape.com From zhangl <@t> utas.edu.au Tue Jul 13 00:06:05 2004 From: zhangl <@t> utas.edu.au (zhangl) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] mapping capillary perfusion pattern Message-ID: <5.1.0.14.0.20040713140603.00af1b90@postoffice.sandybay.utas.edu.au> Hi Histonetters, I am currently doing a project to map the capillary perfusion pattern in perfused rat hindlimb. In this system, aorta and vena carva are cannulated and single hindlimb is perfused wtih BSA buffer. What I have tried was to infuse GSL-1 ( a glycoprotein shown to specifically bound to small vessels ) for 30min, thus those perfused capillaries would be labeled with GSL-1. Then I cut frozen muscle sections and stain them with GSL-1 antibobidies etc. What I found was nearly all available capillaries were labeled. I suspect that since GSL-1 was infused for so long time, some capillaries having trivial flow thus not being functionally perfused were labeled as well. This method could give me overestimated number of perfused capillaries. Has anyone have similar experience? Next idea is to perfuse 2.5% glutaraldehyde at constant pressure for 5min. Then I stain frozen muscle sections with GSL-1. Those perfused capillared will be fixed and keep open, unperfued capillaries would remain closed and appear as a dark dot on the sections. During the perfusion fixation, perfusion pressure goes up presumably due to the cross-linkage in perfused vessels, I need to reduce the perfusion flow rate accordingly to keep pressure constant, also not let red blood cell be washed out because this means recruiting unperfused capillaries, in turn altering flow pattern. Although glutaraldehyde is perfused for a fixed period, the total amount glutaraldehyde going through vascular bed is different for each experiment due to the variation in perfusion flow. My question is in my case whether the time of perfusion fixation or the amount of fixative mainly determines the degree of fixation? Has anyone similar experience? any suggestion and advice is greatly appreciated. Lei Zhang Biochemistry, medicine school University of Tasmania, Australia 61 03 6226 2669 zhangl@utas.edu.au From marshall <@t> cormack.uct.ac.za Tue Jul 13 07:06:17 2004 From: marshall <@t> cormack.uct.ac.za (Marshall) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] eosin fading Message-ID: <40F3D039.C1EA41D7@cormack.uct.ac.za> Hi all, Would like an opinion on what causes eosin to fade while we are on the fading of hematoxylin debate. I find that when I stain a batch of slides 6 months to a year later some of these slides are a lot paler as regards eosin. I then restain and the slide is fine again. I use a eosin/phloxine mixture. Would using an alcoholic eosin mixture improve this? I am using entellan as mounting medium. Thanks sharon marshall e-mail : marshall@cormack.uct.ac.za Dept. Human Biology University of Cape Town South Africa From mward <@t> wfubmc.edu Tue Jul 13 07:19:41 2004 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Rick antibody Message-ID: <61135F0455D33347B5AAE209B903A304076A4EC6@EXCHVS2.medctr.ad.wfubmc.edu> I am going to post this question again since I got no response last week. I am looking for an alternative source for the R. ricksettsi antibody. We used to get it from the CDC but I understand from a colleague that it is not available from them. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From kwittle <@t> jhmi.edu Tue Jul 13 07:42:34 2004 From: kwittle <@t> jhmi.edu (Karen Wittler) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Re: Prostate Biopsies Message-ID: We use a biopsy program of approx. 90 minutes, and stain with additional time in Hematoxylin. Karen Wittler, HT(ASCP) Johns Hopkins Hospital Baltimore, MD >>> histonet-request@lists.utsouthwestern.edu 07/10/04 01:05PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] (tony.j.savage@gsk.com) 2. (no subject) (Achstetter, Virginia A.) 3. Mohs Histotech; Xylene substitute (Michael Todd) 4. DEZENKERIZE USING SAKURA DRS STAINER (Shelkowsky, Ben) 5. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER (Fred Underwood) 6. Re: (no subject) (SMITH,REBEKAH FELICIA) 7. re: 266mp manual (Pereira, Laurie ) 8. RE: B-Plus substitute for B5 (Moklebust, Amanda C) 9. unsubscribe (ANN MARUSKA) 10. RE: B-Plus substitute for B5 (Wiese, Jason VHAROS) 11. RE: B-Plus substitute for B5 (Luck, Greg D.) 12. Re: Room Temperature (Muhammad Tahseen) 13. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER (lpwenk@sbcglobal.net) 14. Re: Room Temperature (lpwenk@sbcglobal.net) 15. PROSTATE BIOSIES (Winters, Bert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 9 Jul 2004 18:41:44 +0100 From: tony.j.savage@gsk.com Subject: [Histonet] Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii From: Juile Gorenstein wrote "Hello all, I was wondering if anyone has had problems with xylene fumes using Sakura VIP Tissue processor. Ours is currently sitting on a bench in a room with a snorkle right above is and the smell is still really bad and is causing headaches for people who try to work in that room. Does anyone have any suggestions? Thank you, Julie Novartis Institues for Biomedical Research Cambridge, MA" It sounds as though it is time to replace the carbon filter on the VIP processor. This should be done on a regular basis depending on usage. Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm ------------------------------ Message: 2 Date: Fri, 9 Jul 2004 14:53:10 -0400 From: "Achstetter, Virginia A." Subject: [Histonet] (no subject) To: "Histonet \(E-mail\)" Message-ID: <9C631520464F9E4BA5B111450F2A0AAD0BC9FB@lewis.afip.osd.mil> Content-Type: text/plain; charset="iso-8859-1" Our department wants to purchase a tissue arrayer from Beecher Instruments. All attempts to contact them has failed. Email address is no longer valid. Fax goes unanswered as well as phone and snail mail. Does anyone know where we could purchase this equipment or how to get in touch with Beecher Instruments? Ginny Achstetter HT (ASCP) Armed Forces Institute of Pathology Soft Tissue Pathology 6825 16th St. NW Bldg 54 Rm. 3118 Washington, DC 20306 202-782-2813 ------------------------------ Message: 3 Date: Fri, 9 Jul 2004 15:09:24 -0400 From: "Michael Todd" Subject: [Histonet] Mohs Histotech; Xylene substitute To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 I am opening a Mohs laboratory in Northern Virginia, just outside Washington DC, and need some help. 1. I need a Histotech, either PT or FT. Beginning September, 2004. 2. I have been following the conversation about xylene substitutes - and from what I gather, they can do a good job. I am wondering if anyone has used "Slide Brite." What the sales rep tells me sounds great, but can anyone speak from their own experience? Thanks Mike Todd Skin Cancer Center of Northern Virginia Sent by Medscape Mail: Free Portable E-mail for Professionals on the Move http://www.medscape.com ------------------------------ Message: 4 Date: Fri, 9 Jul 2004 12:21:01 -0700 From: "Shelkowsky, Ben" Subject: [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D612F@exchsrvr.chomp.org> Content-Type: text/plain; charset="utf-8" I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. ------------------------------ Message: 5 Date: Fri, 09 Jul 2004 15:36:53 -0400 From: "Fred Underwood" Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: Message-ID: Content-Type: text/plain; charset=US-ASCII Set up a reagent station with idodine, 5 minutes shoud do. From there just go through your rehydrating alcohols, tap water wash, and continue your staining procedure. Fred >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 9 Jul 2004 17:06:19 -0400 (EDT) From: "SMITH,REBEKAH FELICIA" Subject: Re: [Histonet] (no subject) To: JAnorthernexp@cs.com, Histonet@lists.utsouthwestern.edu Message-ID: <622625536.1089407179226.JavaMail.osg@spnode33> Content-Type: text/plain; format=flowed; charset=us-ascii Which system? On Wed Jul 07 04:35:02 EDT 2004, JAnorthernexp@cs.com wrote: > Hi there, > Does anyone out there use this system and what is your procedure > for cutting (micron thickness), drying time and temperature for > slides or do you air dry using charge slides. And do you use a > decloaker for deparaffinizing and antigen retrieval. thanks, > > Annette Hart, HT (ASCP) > Wyoming Valley Health Care System > Wilkes-Barre, PA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" ------------------------------ Message: 7 Date: Fri, 9 Jul 2004 14:28:24 -0700 From: "Pereira, Laurie " Subject: [Histonet] re: 266mp manual To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have a manual for the fisher tissue processor 166/266mp. The manual is approximately 59 pages long and I could make a copy. Just let me know where to send it to. Laurie Pereira SDCADDL (Office of the County Veterinarian) laurie.pereira@sdcounty.ca.gov ------------------------------ Message: 8 Date: Fri, 9 Jul 2004 14:27:04 -0700 From: "Moklebust, Amanda C" Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF306@wala01.seattlecca.org> Content-Type: text/plain; charset="iso-8859-1" It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ------------------------------ Message: 9 Date: Fri, 09 Jul 2004 17:41:27 -0500 From: "ANN MARUSKA" Subject: [Histonet] unsubscribe To: Message-ID: Content-Type: text/plain; charset=US-ASCII please unsubscribe The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> ------------------------------ Message: 10 Date: Fri, 9 Jul 2004 15:59:14 -0700 From: "Wiese, Jason VHAROS" Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Moklebust, Amanda C'" , "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 9 Jul 2004 17:19:04 -0700 From: "Luck, Greg D." Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Wiese, Jason VHAROS'" , "'Moklebust, Amanda C'" , "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use "Prefer" and/or "Z-Fix" as our B-5 replacements. Both are manufactured by Anatech. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@med.va.gov] Sent: Friday, July 09, 2004 3:59 PM To: 'Moklebust, Amanda C'; 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Sat, 10 Jul 2004 06:14:54 +0500 From: "Muhammad Tahseen" Subject: Re: [Histonet] Room Temperature To: "Lab Histologia" , Message-ID: <008001c4661b$50e8ca00$972bfea9@User> Content-Type: text/plain; charset="iso-8859-1" 25c ----- Original Message ----- From: Lab Histologia To: Sent: Thursday, July 08, 2004 7:01 PM Subject: [Histonet] Room Temperature > Hello, everyone, > > I need know that range of temperature defines you as room temperature. > > Thanks > > > Hugo > > ------------------------------------------------------------------- > > Dr. Hugo H. Ortega (DMV, PhD) > > Departament of Cellular Biology > > Faculty of Veterinary Sciences > > Universidad Nacional del Litoral > > R.P. Kreder 2805 - Esperanza (3080) > > Santa Fe - ARGENTINA > > Tel. (54)3496-420639 > > Fax. (54)3496-426304 > > http://fcv.unl.edu.ar/histolog/ > > http://fcv.unl.edu.ar/bioterio/ > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Sat, 10 Jul 2004 05:57:51 -0400 From: Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: "Fred Underwood" , Message-ID: <003c01c46664$5d32f580$9f27d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Reminder to everyone who is dezenkerizing (lugolizing) on the stainers - when you get ready to dump this iodine station, it cannot go down the sink. It now contains mercuric salts, so must be collected as mercury waste. (As should the next couple of containers of reagents, carry-over on the slides.) Ways to get around this: 1. Dezenkerize (lugolize) by hand, then add the slide to the stainer. 2. Switch over to a zinc fixative. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Fred Underwood" To: Sent: Friday, July 09, 2004 3:36 PM Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER > Set up a reagent station with idodine, 5 minutes shoud do. From there > just go through your rehydrating alcohols, tap water wash, and continue > your staining procedure. > > Fred > > >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> > I would like to dezenkerize my slides from mercury-fixed tissues on the > SAKURA automated stainer in a program I can internalize on the menu. Is > anyone out there already doing this and can you send me your procedure? > > We would appreciate any help on this matter. > > Ben Shelkowsky > CHOMP > Monterey, CA. 93950 > ben.shelkowsky@chomp.org > Confidentiality Notice: > This is a transmission from Community Hospital of the Monterey > Peninsula. This message and any attached documents may be confidential > and contain information protected by state and federal medical privacy > statutes. They are intended only for the use of the addressee. If you > are not the intended recipient, any disclosure, copying, or distribution > of this information is strictly prohibited. If you received this > transmission in error, please accept our apologies and notify the > sender. > > Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Sat, 10 Jul 2004 06:08:34 -0400 From: Subject: Re: [Histonet] Room Temperature To: "Muhammad Tahseen" , "Lab Histologia" , Message-ID: <004501c46665$dcc2cae0$9f27d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Take the temperature of YOUR room, and use that. Muhammad said 25 degrees C. 25 degrees C = 77 degrees F That would be too hot in our lab in Michigan, which is air conditioned in the spring/summer/fall, and never too hot when the furnace is on in the winter. Plus, with the increased air ventilation/flow, it always seems chillier. 22 degrees C = 71.6 degrees F. 20 degrees C = 68 degrees F. That's more the range in our anatomic pathology labs. Of course, one year they move the student lab into a room that the heat didn't work very well. We did have a large picture window, but there was a sheet of ice on it all winter long. Our average that winter was: 15 degrees C = 59 degrees F Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Muhammad Tahseen" To: "Lab Histologia" ; Sent: Friday, July 09, 2004 9:14 PM Subject: Re: [Histonet] Room Temperature > 25c > ----- Original Message ----- > From: Lab Histologia > To: > Sent: Thursday, July 08, 2004 7:01 PM > Subject: [Histonet] Room Temperature > > > > Hello, everyone, > > > > I need know that range of temperature defines you as room temperature. > > > > Thanks > > > > > > Hugo > > > > ------------------------------------------------------------------- > > > > Dr. Hugo H. Ortega (DMV, PhD) > > > > Departament of Cellular Biology > > > > Faculty of Veterinary Sciences > > > > Universidad Nacional del Litoral > > > > R.P. Kreder 2805 - Esperanza (3080) > > > > Santa Fe - ARGENTINA > > > > Tel. (54)3496-420639 > > > > Fax. (54)3496-426304 > > > > http://fcv.unl.edu.ar/histolog/ > > > > http://fcv.unl.edu.ar/bioterio/ > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Sat, 10 Jul 2004 07:37:57 -0500 From: "Winters, Bert" Subject: [Histonet] PROSTATE BIOSIES To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC362776D@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" I am looking for any suggestions when it comes to processing prostate biopsies. The pathologist have going back and forth with processing these biopsies using a typical biopsy program on the tissue processors and using a routine process which is used for processing most tissue specimens. Everyone I have talked with about this processes all prostate biopsies using a biopsy program, about 5-6 hours in the tissue processor. Any processing or staining tips anyone could give me about prostate biopsies would be greatly appreciated. Bert ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 16 *************************************** From kwittle <@t> jhmi.edu Tue Jul 13 08:09:56 2004 From: kwittle <@t> jhmi.edu (Karen Wittler) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Re: Dezenkerize Message-ID: To remove Mercury precipitate pigments we use iodine in xylene when the slides come out of the oven, then deparaffinize and stain as usual. I imagine this would work with an automated stainer. Karen Wittler, HT(ASCP) Johns Hopkins Hospital Baltimore, MD >>> histonet-request@lists.utsouthwestern.edu 07/10/04 01:05PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] (tony.j.savage@gsk.com) 2. (no subject) (Achstetter, Virginia A.) 3. Mohs Histotech; Xylene substitute (Michael Todd) 4. DEZENKERIZE USING SAKURA DRS STAINER (Shelkowsky, Ben) 5. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER (Fred Underwood) 6. Re: (no subject) (SMITH,REBEKAH FELICIA) 7. re: 266mp manual (Pereira, Laurie ) 8. RE: B-Plus substitute for B5 (Moklebust, Amanda C) 9. unsubscribe (ANN MARUSKA) 10. RE: B-Plus substitute for B5 (Wiese, Jason VHAROS) 11. RE: B-Plus substitute for B5 (Luck, Greg D.) 12. Re: Room Temperature (Muhammad Tahseen) 13. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER (lpwenk@sbcglobal.net) 14. Re: Room Temperature (lpwenk@sbcglobal.net) 15. PROSTATE BIOSIES (Winters, Bert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 9 Jul 2004 18:41:44 +0100 From: tony.j.savage@gsk.com Subject: [Histonet] Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii From: Juile Gorenstein wrote "Hello all, I was wondering if anyone has had problems with xylene fumes using Sakura VIP Tissue processor. Ours is currently sitting on a bench in a room with a snorkle right above is and the smell is still really bad and is causing headaches for people who try to work in that room. Does anyone have any suggestions? Thank you, Julie Novartis Institues for Biomedical Research Cambridge, MA" It sounds as though it is time to replace the carbon filter on the VIP processor. This should be done on a regular basis depending on usage. Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm ------------------------------ Message: 2 Date: Fri, 9 Jul 2004 14:53:10 -0400 From: "Achstetter, Virginia A." Subject: [Histonet] (no subject) To: "Histonet \(E-mail\)" Message-ID: <9C631520464F9E4BA5B111450F2A0AAD0BC9FB@lewis.afip.osd.mil> Content-Type: text/plain; charset="iso-8859-1" Our department wants to purchase a tissue arrayer from Beecher Instruments. All attempts to contact them has failed. Email address is no longer valid. Fax goes unanswered as well as phone and snail mail. Does anyone know where we could purchase this equipment or how to get in touch with Beecher Instruments? Ginny Achstetter HT (ASCP) Armed Forces Institute of Pathology Soft Tissue Pathology 6825 16th St. NW Bldg 54 Rm. 3118 Washington, DC 20306 202-782-2813 ------------------------------ Message: 3 Date: Fri, 9 Jul 2004 15:09:24 -0400 From: "Michael Todd" Subject: [Histonet] Mohs Histotech; Xylene substitute To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 I am opening a Mohs laboratory in Northern Virginia, just outside Washington DC, and need some help. 1. I need a Histotech, either PT or FT. Beginning September, 2004. 2. I have been following the conversation about xylene substitutes - and from what I gather, they can do a good job. I am wondering if anyone has used "Slide Brite." What the sales rep tells me sounds great, but can anyone speak from their own experience? Thanks Mike Todd Skin Cancer Center of Northern Virginia Sent by Medscape Mail: Free Portable E-mail for Professionals on the Move http://www.medscape.com ------------------------------ Message: 4 Date: Fri, 9 Jul 2004 12:21:01 -0700 From: "Shelkowsky, Ben" Subject: [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D612F@exchsrvr.chomp.org> Content-Type: text/plain; charset="utf-8" I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. ------------------------------ Message: 5 Date: Fri, 09 Jul 2004 15:36:53 -0400 From: "Fred Underwood" Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: Message-ID: Content-Type: text/plain; charset=US-ASCII Set up a reagent station with idodine, 5 minutes shoud do. From there just go through your rehydrating alcohols, tap water wash, and continue your staining procedure. Fred >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 9 Jul 2004 17:06:19 -0400 (EDT) From: "SMITH,REBEKAH FELICIA" Subject: Re: [Histonet] (no subject) To: JAnorthernexp@cs.com, Histonet@lists.utsouthwestern.edu Message-ID: <622625536.1089407179226.JavaMail.osg@spnode33> Content-Type: text/plain; format=flowed; charset=us-ascii Which system? On Wed Jul 07 04:35:02 EDT 2004, JAnorthernexp@cs.com wrote: > Hi there, > Does anyone out there use this system and what is your procedure > for cutting (micron thickness), drying time and temperature for > slides or do you air dry using charge slides. And do you use a > decloaker for deparaffinizing and antigen retrieval. thanks, > > Annette Hart, HT (ASCP) > Wyoming Valley Health Care System > Wilkes-Barre, PA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" ------------------------------ Message: 7 Date: Fri, 9 Jul 2004 14:28:24 -0700 From: "Pereira, Laurie " Subject: [Histonet] re: 266mp manual To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have a manual for the fisher tissue processor 166/266mp. The manual is approximately 59 pages long and I could make a copy. Just let me know where to send it to. Laurie Pereira SDCADDL (Office of the County Veterinarian) laurie.pereira@sdcounty.ca.gov ------------------------------ Message: 8 Date: Fri, 9 Jul 2004 14:27:04 -0700 From: "Moklebust, Amanda C" Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF306@wala01.seattlecca.org> Content-Type: text/plain; charset="iso-8859-1" It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ------------------------------ Message: 9 Date: Fri, 09 Jul 2004 17:41:27 -0500 From: "ANN MARUSKA" Subject: [Histonet] unsubscribe To: Message-ID: Content-Type: text/plain; charset=US-ASCII please unsubscribe The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> ------------------------------ Message: 10 Date: Fri, 9 Jul 2004 15:59:14 -0700 From: "Wiese, Jason VHAROS" Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Moklebust, Amanda C'" , "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 9 Jul 2004 17:19:04 -0700 From: "Luck, Greg D." Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Wiese, Jason VHAROS'" , "'Moklebust, Amanda C'" , "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use "Prefer" and/or "Z-Fix" as our B-5 replacements. Both are manufactured by Anatech. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@med.va.gov] Sent: Friday, July 09, 2004 3:59 PM To: 'Moklebust, Amanda C'; 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Sat, 10 Jul 2004 06:14:54 +0500 From: "Muhammad Tahseen" Subject: Re: [Histonet] Room Temperature To: "Lab Histologia" , Message-ID: <008001c4661b$50e8ca00$972bfea9@User> Content-Type: text/plain; charset="iso-8859-1" 25c ----- Original Message ----- From: Lab Histologia To: Sent: Thursday, July 08, 2004 7:01 PM Subject: [Histonet] Room Temperature > Hello, everyone, > > I need know that range of temperature defines you as room temperature. > > Thanks > > > Hugo > > ------------------------------------------------------------------- > > Dr. Hugo H. Ortega (DMV, PhD) > > Departament of Cellular Biology > > Faculty of Veterinary Sciences > > Universidad Nacional del Litoral > > R.P. Kreder 2805 - Esperanza (3080) > > Santa Fe - ARGENTINA > > Tel. (54)3496-420639 > > Fax. (54)3496-426304 > > http://fcv.unl.edu.ar/histolog/ > > http://fcv.unl.edu.ar/bioterio/ > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Sat, 10 Jul 2004 05:57:51 -0400 From: Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: "Fred Underwood" , Message-ID: <003c01c46664$5d32f580$9f27d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Reminder to everyone who is dezenkerizing (lugolizing) on the stainers - when you get ready to dump this iodine station, it cannot go down the sink. It now contains mercuric salts, so must be collected as mercury waste. (As should the next couple of containers of reagents, carry-over on the slides.) Ways to get around this: 1. Dezenkerize (lugolize) by hand, then add the slide to the stainer. 2. Switch over to a zinc fixative. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Fred Underwood" To: Sent: Friday, July 09, 2004 3:36 PM Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER > Set up a reagent station with idodine, 5 minutes shoud do. From there > just go through your rehydrating alcohols, tap water wash, and continue > your staining procedure. > > Fred > > >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> > I would like to dezenkerize my slides from mercury-fixed tissues on the > SAKURA automated stainer in a program I can internalize on the menu. Is > anyone out there already doing this and can you send me your procedure? > > We would appreciate any help on this matter. > > Ben Shelkowsky > CHOMP > Monterey, CA. 93950 > ben.shelkowsky@chomp.org > Confidentiality Notice: > This is a transmission from Community Hospital of the Monterey > Peninsula. This message and any attached documents may be confidential > and contain information protected by state and federal medical privacy > statutes. They are intended only for the use of the addressee. If you > are not the intended recipient, any disclosure, copying, or distribution > of this information is strictly prohibited. If you received this > transmission in error, please accept our apologies and notify the > sender. > > Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Sat, 10 Jul 2004 06:08:34 -0400 From: Subject: Re: [Histonet] Room Temperature To: "Muhammad Tahseen" , "Lab Histologia" , Message-ID: <004501c46665$dcc2cae0$9f27d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Take the temperature of YOUR room, and use that. Muhammad said 25 degrees C. 25 degrees C = 77 degrees F That would be too hot in our lab in Michigan, which is air conditioned in the spring/summer/fall, and never too hot when the furnace is on in the winter. Plus, with the increased air ventilation/flow, it always seems chillier. 22 degrees C = 71.6 degrees F. 20 degrees C = 68 degrees F. That's more the range in our anatomic pathology labs. Of course, one year they move the student lab into a room that the heat didn't work very well. We did have a large picture window, but there was a sheet of ice on it all winter long. Our average that winter was: 15 degrees C = 59 degrees F Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Muhammad Tahseen" To: "Lab Histologia" ; Sent: Friday, July 09, 2004 9:14 PM Subject: Re: [Histonet] Room Temperature > 25c > ----- Original Message ----- > From: Lab Histologia > To: > Sent: Thursday, July 08, 2004 7:01 PM > Subject: [Histonet] Room Temperature > > > > Hello, everyone, > > > > I need know that range of temperature defines you as room temperature. > > > > Thanks > > > > > > Hugo > > > > ------------------------------------------------------------------- > > > > Dr. Hugo H. Ortega (DMV, PhD) > > > > Departament of Cellular Biology > > > > Faculty of Veterinary Sciences > > > > Universidad Nacional del Litoral > > > > R.P. Kreder 2805 - Esperanza (3080) > > > > Santa Fe - ARGENTINA > > > > Tel. (54)3496-420639 > > > > Fax. (54)3496-426304 > > > > http://fcv.unl.edu.ar/histolog/ > > > > http://fcv.unl.edu.ar/bioterio/ > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Sat, 10 Jul 2004 07:37:57 -0500 From: "Winters, Bert" Subject: [Histonet] PROSTATE BIOSIES To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC362776D@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" I am looking for any suggestions when it comes to processing prostate biopsies. The pathologist have going back and forth with processing these biopsies using a typical biopsy program on the tissue processors and using a routine process which is used for processing most tissue specimens. Everyone I have talked with about this processes all prostate biopsies using a biopsy program, about 5-6 hours in the tissue processor. Any processing or staining tips anyone could give me about prostate biopsies would be greatly appreciated. Bert ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 16 *************************************** From ione.jackman <@t> uconn.edu Mon Jul 12 15:14:33 2004 From: ione.jackman <@t> uconn.edu (ione jackman) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] nair, exoskeletons and IHC In-Reply-To: Message-ID: Hi Sarah, I did a short in-house study several years ago using Nair, Neet and Mollifax as softening agents. I found slightly diminished staining with the Nair and Neet products. DL. Woodward UCONN From minkk <@t> zgi.com Mon Jul 12 19:09:48 2004 From: minkk <@t> zgi.com (KMIN (Kathy Mink)) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink From funderwood <@t> mcohio.org Tue Jul 13 08:41:08 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:43 2005 Subject: [BULK] - [Histonet] Formalin fix---then freeze? Message-ID: Hi Kathy, It is possible. Though, depending on the tissue, it is more difficult to section. Like lung for example. I usually end up grubbing tissue out of the stock bag when a fat stain is requested. I would recommend washing the tissue thoroughly before freezing. Fred >>> "KMIN (Kathy Mink)" 07/12/04 08:09PM >>> Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pam <@t> ategra.com Tue Jul 13 08:53:04 2004 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Histology Opportunities in California and Georgia Message-ID: Hello Histonetters - I am presently on a search for several of my best clients in Northern California and Northeast Georgia who are seeking to hire Histo techs. These are full time permanent positions. The clients are offering relocation assistance, great benefits and competitive salary commensurate with experience Are you interested ? Also, if you have friends/peers who might be interested, if you could pass my query & name on to them I'd be much obliged. If interested, please call me at 800-466-9919 x234. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: http://www.ategra.com Ategra Systems Inc Specialists in Permanent & Contract Staffing 7085 University Blvd Winter Park, FL 32792-6721 800-466-9919 ext 234 EMAIL: pam@ategra.com WEBSITE: http://www.ategra.com From juan.gutierrez <@t> christushealth.org Tue Jul 13 09:01:14 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: Yes! I used to get this request quite often. Just trim the tissue you're going to freeze, put it in a cassette and wash it under running deionized water for a couple of hours. Pat it dry and embed in OCT. I've done it with heart, lung and liver and it works very well. Good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: KMIN (Kathy Mink) [mailto:minkk@zgi.com] Sent: Monday, July 12, 2004 7:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Jul 13 09:02:45 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Re: Dezenkerize Message-ID: Just curious. How do you mixed the iodine with the xylene? Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Karen Wittler [mailto:kwittle@jhmi.edu] Sent: Tuesday, July 13, 2004 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Dezenkerize To remove Mercury precipitate pigments we use iodine in xylene when the slides come out of the oven, then deparaffinize and stain as usual. I imagine this would work with an automated stainer. Karen Wittler, HT(ASCP) Johns Hopkins Hospital Baltimore, MD >>> histonet-request@lists.utsouthwestern.edu 07/10/04 01:05PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] (tony.j.savage@gsk.com) 2. (no subject) (Achstetter, Virginia A.) 3. Mohs Histotech; Xylene substitute (Michael Todd) 4. DEZENKERIZE USING SAKURA DRS STAINER (Shelkowsky, Ben) 5. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER (Fred Underwood) 6. Re: (no subject) (SMITH,REBEKAH FELICIA) 7. re: 266mp manual (Pereira, Laurie ) 8. RE: B-Plus substitute for B5 (Moklebust, Amanda C) 9. unsubscribe (ANN MARUSKA) 10. RE: B-Plus substitute for B5 (Wiese, Jason VHAROS) 11. RE: B-Plus substitute for B5 (Luck, Greg D.) 12. Re: Room Temperature (Muhammad Tahseen) 13. Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER (lpwenk@sbcglobal.net) 14. Re: Room Temperature (lpwenk@sbcglobal.net) 15. PROSTATE BIOSIES (Winters, Bert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 9 Jul 2004 18:41:44 +0100 From: tony.j.savage@gsk.com Subject: [Histonet] Re: Sakura Tissue Processor [Histonet Digest, Vol 8, Issue 15] To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii From: Juile Gorenstein wrote "Hello all, I was wondering if anyone has had problems with xylene fumes using Sakura VIP Tissue processor. Ours is currently sitting on a bench in a room with a snorkle right above is and the smell is still really bad and is causing headaches for people who try to work in that room. Does anyone have any suggestions? Thank you, Julie Novartis Institues for Biomedical Research Cambridge, MA" It sounds as though it is time to replace the carbon filter on the VIP processor. This should be done on a regular basis depending on usage. Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm ------------------------------ Message: 2 Date: Fri, 9 Jul 2004 14:53:10 -0400 From: "Achstetter, Virginia A." Subject: [Histonet] (no subject) To: "Histonet \(E-mail\)" Message-ID: <9C631520464F9E4BA5B111450F2A0AAD0BC9FB@lewis.afip.osd.mil> Content-Type: text/plain; charset="iso-8859-1" Our department wants to purchase a tissue arrayer from Beecher Instruments. All attempts to contact them has failed. Email address is no longer valid. Fax goes unanswered as well as phone and snail mail. Does anyone know where we could purchase this equipment or how to get in touch with Beecher Instruments? Ginny Achstetter HT (ASCP) Armed Forces Institute of Pathology Soft Tissue Pathology 6825 16th St. NW Bldg 54 Rm. 3118 Washington, DC 20306 202-782-2813 ------------------------------ Message: 3 Date: Fri, 9 Jul 2004 15:09:24 -0400 From: "Michael Todd" Subject: [Histonet] Mohs Histotech; Xylene substitute To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 I am opening a Mohs laboratory in Northern Virginia, just outside Washington DC, and need some help. 1. I need a Histotech, either PT or FT. Beginning September, 2004. 2. I have been following the conversation about xylene substitutes - and from what I gather, they can do a good job. I am wondering if anyone has used "Slide Brite." What the sales rep tells me sounds great, but can anyone speak from their own experience? Thanks Mike Todd Skin Cancer Center of Northern Virginia Sent by Medscape Mail: Free Portable E-mail for Professionals on the Move http://www.medscape.com ------------------------------ Message: 4 Date: Fri, 9 Jul 2004 12:21:01 -0700 From: "Shelkowsky, Ben" Subject: [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D612F@exchsrvr.chomp.org> Content-Type: text/plain; charset="utf-8" I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. ------------------------------ Message: 5 Date: Fri, 09 Jul 2004 15:36:53 -0400 From: "Fred Underwood" Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: Message-ID: Content-Type: text/plain; charset=US-ASCII Set up a reagent station with idodine, 5 minutes shoud do. From there just go through your rehydrating alcohols, tap water wash, and continue your staining procedure. Fred >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> I would like to dezenkerize my slides from mercury-fixed tissues on the SAKURA automated stainer in a program I can internalize on the menu. Is anyone out there already doing this and can you send me your procedure? We would appreciate any help on this matter. Ben Shelkowsky CHOMP Monterey, CA. 93950 ben.shelkowsky@chomp.org Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 9 Jul 2004 17:06:19 -0400 (EDT) From: "SMITH,REBEKAH FELICIA" Subject: Re: [Histonet] (no subject) To: JAnorthernexp@cs.com, Histonet@lists.utsouthwestern.edu Message-ID: <622625536.1089407179226.JavaMail.osg@spnode33> Content-Type: text/plain; format=flowed; charset=us-ascii Which system? On Wed Jul 07 04:35:02 EDT 2004, JAnorthernexp@cs.com wrote: > Hi there, > Does anyone out there use this system and what is your procedure > for cutting (micron thickness), drying time and temperature for > slides or do you air dry using charge slides. And do you use a > decloaker for deparaffinizing and antigen retrieval. thanks, > > Annette Hart, HT (ASCP) > Wyoming Valley Health Care System > Wilkes-Barre, PA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" ------------------------------ Message: 7 Date: Fri, 9 Jul 2004 14:28:24 -0700 From: "Pereira, Laurie " Subject: [Histonet] re: 266mp manual To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have a manual for the fisher tissue processor 166/266mp. The manual is approximately 59 pages long and I could make a copy. Just let me know where to send it to. Laurie Pereira SDCADDL (Office of the County Veterinarian) laurie.pereira@sdcounty.ca.gov ------------------------------ Message: 8 Date: Fri, 9 Jul 2004 14:27:04 -0700 From: "Moklebust, Amanda C" Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948013CF306@wala01.seattlecca.org> Content-Type: text/plain; charset="iso-8859-1" It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ------------------------------ Message: 9 Date: Fri, 09 Jul 2004 17:41:27 -0500 From: "ANN MARUSKA" Subject: [Histonet] unsubscribe To: Message-ID: Content-Type: text/plain; charset=US-ASCII please unsubscribe The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> ------------------------------ Message: 10 Date: Fri, 9 Jul 2004 15:59:14 -0700 From: "Wiese, Jason VHAROS" Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Moklebust, Amanda C'" , "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 9 Jul 2004 17:19:04 -0700 From: "Luck, Greg D." Subject: RE: [Histonet] B-Plus substitute for B5 To: "'Wiese, Jason VHAROS'" , "'Moklebust, Amanda C'" , "'Gladney, Diane C Ms MACH'" , Joe Nocito , Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use "Prefer" and/or "Z-Fix" as our B-5 replacements. Both are manufactured by Anatech. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@med.va.gov] Sent: Friday, July 09, 2004 3:59 PM To: 'Moklebust, Amanda C'; 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 I use Presto-fixe II(B-5 replacement). I purchase it from Spectra Tint (Phone 800-888-8468... Fax 716-546-7979) Item #F112 It may not work for your needs, depending on what they are, but it is a good product to use in a comparison study. Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Moklebust, Amanda C [mailto:amoklebu@seattlecca.org] Sent: Friday, July 09, 2004 2:27 PM To: 'Gladney, Diane C Ms MACH'; Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 It is good to hear that people are finding an adequate replacement for B-5. Before our lab can make the change the pathologists want to see a comparison study of at least two fixatives in addition to B-5. Can anyone pass on the names of other B-5 substitutes? Thanks for the help. Amanda Moklebust, HTL Pathology Laboratory Seattle Cancer Care Alliance Seattle, Washington -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Thursday, July 08, 2004 1:12 PM To: Joe Nocito; Histonet Subject: RE: [Histonet] B-Plus substitute for B5 Joe, We have been using B-Plus for about 2 years. Our pathologists love it and feel that it is equal to B-5. We have been required to eliminate the use of mercury products in our lab so this product has worked very exceptional for us. Trying to dispose of the mercury waste was getting to be a nightmare. Our pathologists only commented right after we started using this product that they noticed a very slight difference. They felt the difference was insignificant and adjusted quickly to the slight difference. Now they say that they can't tell the difference.....hmmm. We had tried another product before discovering B-Plus that they just hated. I was thrilled that they accepted the B-Plus as I was out of options to try for a B-5 substitute. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, July 08, 2004 10:38 AM To: Histonet Subject: [Histonet] B-Plus substitute for B5 Good morning Histonetters, My hempath is asking me to look into a substance called B-plus? It's supposed to replace B5. Any information will be greatly appreciated. Thanks Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Sat, 10 Jul 2004 06:14:54 +0500 From: "Muhammad Tahseen" Subject: Re: [Histonet] Room Temperature To: "Lab Histologia" , Message-ID: <008001c4661b$50e8ca00$972bfea9@User> Content-Type: text/plain; charset="iso-8859-1" 25c ----- Original Message ----- From: Lab Histologia To: Sent: Thursday, July 08, 2004 7:01 PM Subject: [Histonet] Room Temperature > Hello, everyone, > > I need know that range of temperature defines you as room temperature. > > Thanks > > > Hugo > > ------------------------------------------------------------------- > > Dr. Hugo H. Ortega (DMV, PhD) > > Departament of Cellular Biology > > Faculty of Veterinary Sciences > > Universidad Nacional del Litoral > > R.P. Kreder 2805 - Esperanza (3080) > > Santa Fe - ARGENTINA > > Tel. (54)3496-420639 > > Fax. (54)3496-426304 > > http://fcv.unl.edu.ar/histolog/ > > http://fcv.unl.edu.ar/bioterio/ > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Sat, 10 Jul 2004 05:57:51 -0400 From: Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER To: "Fred Underwood" , Message-ID: <003c01c46664$5d32f580$9f27d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Reminder to everyone who is dezenkerizing (lugolizing) on the stainers - when you get ready to dump this iodine station, it cannot go down the sink. It now contains mercuric salts, so must be collected as mercury waste. (As should the next couple of containers of reagents, carry-over on the slides.) Ways to get around this: 1. Dezenkerize (lugolize) by hand, then add the slide to the stainer. 2. Switch over to a zinc fixative. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Fred Underwood" To: Sent: Friday, July 09, 2004 3:36 PM Subject: Re: [BULK] - [Histonet] DEZENKERIZE USING SAKURA DRS STAINER > Set up a reagent station with idodine, 5 minutes shoud do. From there > just go through your rehydrating alcohols, tap water wash, and continue > your staining procedure. > > Fred > > >>> "Shelkowsky, Ben" 07/09/04 03:21PM >>> > I would like to dezenkerize my slides from mercury-fixed tissues on the > SAKURA automated stainer in a program I can internalize on the menu. Is > anyone out there already doing this and can you send me your procedure? > > We would appreciate any help on this matter. > > Ben Shelkowsky > CHOMP > Monterey, CA. 93950 > ben.shelkowsky@chomp.org > Confidentiality Notice: > This is a transmission from Community Hospital of the Monterey > Peninsula. This message and any attached documents may be confidential > and contain information protected by state and federal medical privacy > statutes. They are intended only for the use of the addressee. If you > are not the intended recipient, any disclosure, copying, or distribution > of this information is strictly prohibited. If you received this > transmission in error, please accept our apologies and notify the > sender. > > Thank you. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Sat, 10 Jul 2004 06:08:34 -0400 From: Subject: Re: [Histonet] Room Temperature To: "Muhammad Tahseen" , "Lab Histologia" , Message-ID: <004501c46665$dcc2cae0$9f27d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" Take the temperature of YOUR room, and use that. Muhammad said 25 degrees C. 25 degrees C = 77 degrees F That would be too hot in our lab in Michigan, which is air conditioned in the spring/summer/fall, and never too hot when the furnace is on in the winter. Plus, with the increased air ventilation/flow, it always seems chillier. 22 degrees C = 71.6 degrees F. 20 degrees C = 68 degrees F. That's more the range in our anatomic pathology labs. Of course, one year they move the student lab into a room that the heat didn't work very well. We did have a large picture window, but there was a sheet of ice on it all winter long. Our average that winter was: 15 degrees C = 59 degrees F Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Muhammad Tahseen" To: "Lab Histologia" ; Sent: Friday, July 09, 2004 9:14 PM Subject: Re: [Histonet] Room Temperature > 25c > ----- Original Message ----- > From: Lab Histologia > To: > Sent: Thursday, July 08, 2004 7:01 PM > Subject: [Histonet] Room Temperature > > > > Hello, everyone, > > > > I need know that range of temperature defines you as room temperature. > > > > Thanks > > > > > > Hugo > > > > ------------------------------------------------------------------- > > > > Dr. Hugo H. Ortega (DMV, PhD) > > > > Departament of Cellular Biology > > > > Faculty of Veterinary Sciences > > > > Universidad Nacional del Litoral > > > > R.P. Kreder 2805 - Esperanza (3080) > > > > Santa Fe - ARGENTINA > > > > Tel. (54)3496-420639 > > > > Fax. (54)3496-426304 > > > > http://fcv.unl.edu.ar/histolog/ > > > > http://fcv.unl.edu.ar/bioterio/ > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Sat, 10 Jul 2004 07:37:57 -0500 From: "Winters, Bert" Subject: [Histonet] PROSTATE BIOSIES To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC362776D@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" I am looking for any suggestions when it comes to processing prostate biopsies. The pathologist have going back and forth with processing these biopsies using a typical biopsy program on the tissue processors and using a routine process which is used for processing most tissue specimens. Everyone I have talked with about this processes all prostate biopsies using a biopsy program, about 5-6 hours in the tissue processor. Any processing or staining tips anyone could give me about prostate biopsies would be greatly appreciated. Bert ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 16 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PWG1 <@t> CDC.GOV Tue Jul 13 09:01:46 2004 From: PWG1 <@t> CDC.GOV (Greer, Patricia) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: Kathy, Yes, we have frozen formalin fixed tissues in several instances in the past. We would rinse the formalin (or 70% alcohol if that is what the tissue is stored in) and place the tissue in an embedding mold filled with OCT and freeze onto a chuck. This worked well for a number of tissues and was helpful in doing a quick special stain or immunohistochemistry. Pat Greer Centers for Disease Control and Prevention Infectious Disease Pathology Activity 1600 Clifton Road Atlanta, GA 30333 Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? Kathy Mink From mcauliff <@t> umdnj.edu Tue Jul 13 12:09:41 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] mapping capillary perfusion pattern In-Reply-To: <5.1.0.14.0.20040713140603.00af1b90@postoffice.sandybay.utas.edu.au> References: <5.1.0.14.0.20040713140603.00af1b90@postoffice.sandybay.utas.edu.au> Message-ID: <40F41755.4030608@umdnj.edu> Dear Lei: Capillary bed perfusion varies depending on many local (microscopic) factors. Even in a hindlimb at rest capillary perfusion will vary with time and perfusion will depend on factors you probably can't measure. I would suggest not perfusing with anything; clamp the major vessels to trap the blood in the capillary beds. Then either fix or freeze and use benzidine or diaminobenzidine to demonstrate the hemoglobin in the red cells trapped in the capillaries. Someone did this many years ago, published their method in the journal Stain Technology (now known as Biotechnic and Histochemistry), I think they were looking at vasa recta in the kidney. Geoff zhangl wrote: > Hi Histonetters, > > I am currently doing a project to map the capillary perfusion pattern > in perfused rat hindlimb. In this system, aorta and vena carva are > cannulated and single hindlimb is perfused wtih BSA buffer. What I > have tried was to infuse GSL-1 ( a glycoprotein shown to specifically > bound to small vessels ) for 30min, thus those perfused capillaries > would be labeled with GSL-1. Then I cut frozen muscle sections and > stain them with GSL-1 antibobidies etc. What I found was nearly all > available capillaries were labeled. I suspect that since GSL-1 was > infused for so long time, some capillaries having trivial flow thus > not being functionally perfused were labeled as well. This method > could give me overestimated number of perfused capillaries. Has anyone > have similar experience? > > Next idea is to perfuse 2.5% glutaraldehyde at constant pressure for > 5min. Then I stain frozen muscle sections with GSL-1. Those perfused > capillared will be fixed and keep open, unperfued capillaries would > remain closed and appear as a dark dot on the sections. During the > perfusion fixation, perfusion pressure goes up presumably due to the > cross-linkage in perfused vessels, I need to reduce the perfusion flow > rate accordingly to keep pressure constant, also not let red blood > cell be washed out because this means recruiting unperfused > capillaries, in turn altering flow pattern. Although glutaraldehyde is > perfused for a fixed period, the total amount glutaraldehyde going > through vascular bed is different for each experiment due to the > variation in perfusion flow. My question is in my case whether the > time of perfusion fixation or the amount of fixative mainly determines > the degree of fixation? Has anyone similar experience? any suggestion > and advice is greatly appreciated. > > Lei Zhang > Biochemistry, medicine school > University of Tasmania, Australia > 61 03 6226 2669 > zhangl@utas.edu.au > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Ronnie_Houston <@t> bshsi.com Tue Jul 13 08:58:37 2004 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: <530361BF03351B4CAE5270A05D3037B503673390@bsrexms01.BSHSIR.COM> Absolutely; although there is a very real possibility that the tissue will float off the slide (no matter what type of slide you use) if cutting with a cryostat. Wash the tissue well before freezing. The microtome of choice for sectioning frozen fixed tissue is a freezing microtome, although there aren't many about any more. What a shame!....now that is an art and experience that younger histotechs are losing out on....I remember the days of free-floating cerebellum sections................showing my age now. Ronnie Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 2877972 (804) 2877906 - fax ronnie_houston@bshsi.com -----Original Message----- From: KMIN (Kathy Mink) [mailto:minkk@zgi.com] Sent: Monday, July 12, 2004 8:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From mcauliff <@t> umdnj.edu Tue Jul 13 12:30:48 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? In-Reply-To: References: Message-ID: <40F41C48.40607@umdnj.edu> Hi Kathy: This method is very do-able, is common and does make sense; you get the benefits of fixation (better morphology) and benefits of frozen sections (fat stains, immuno stains). I would suggest throwing in a cryoprotection step (20-30% sucrose overnight in the refrigerator) after washing out the formalin and, of course, rapid freezing to avoid ice crystal artifacts. Geoff KMIN (Kathy Mink) wrote: >Hi, > >I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! > >Kathy Mink > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gu.lang <@t> gmx.at Tue Jul 13 09:58:06 2004 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? References: Message-ID: <003501c468e9$ce4d2f10$eeeea8c0@SERVER> Would this method be adequate for lung to perform Sudan-stain on it? That opens the possibility to store the autopsy-tissue in formalin and do the staining later. Has anybody experience with this? Gudrun Lang ----- Original Message ----- From: "KMIN (Kathy Mink)" To: Sent: Tuesday, July 13, 2004 2:09 AM Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adelsyscarol <@t> yahoo.com Tue Jul 13 10:14:02 2004 From: adelsyscarol <@t> yahoo.com (Carol Wilson) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] looking to buy a Cytospin 3 or 4 Message-ID: <20040713151402.18038.qmail@web50601.mail.yahoo.com> Does anyone have a Cytospin 3 or 4 in good condition that they are interested in selling, and if so do you have an asking price? Thank you, Carol Wilson, H.T. (A.S.C.P.) . __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gu.lang <@t> gmx.at Tue Jul 13 10:15:14 2004 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] cd-14 on paraffin, lyme-borreliose-antibody Message-ID: <005b01c468ec$32b9e270$eeeea8c0@SERVER> Hi histonetters I am posting this question for my workmate. She is looking for an CD14-antibody on formalin fixed, paraffin-embed tissue, that is compatible for Ventana-Nexes. Any hints are welcome. ii. Is there an IHC-antibody for lyme-borreliose (borrelia burgdorferi) available (FFPE tissue, especially on skin) thanks in advance Gudrun Lang Akh Linz, Austria From hborgeri <@t> wfubmc.edu Tue Jul 13 10:35:06 2004 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: <9AEEF1FB6254224AA355ED285F8491650999C803@EXCHVS2.medctr.ad.wfubmc.edu> We routinely do lipid staining on formalin fixed archival tissue and use the following procedure: wash the tissue specimen under running water for an hour to remove the formalin. Then infiltrate overnight at 4?C with 15% sucrose in PBS. Freeze the tissue in OCT and section at 5 - 6 ?m. We use Churukian's Oil Red O as our fat stain. By cryoprotecting the tissue in sucrose, the morphology is comparable to that of a paraffin section. Hermina Hermina M. Borgerink, BA, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax (336) 716-1515 e-mail hborgeri@wfubmc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, July 13, 2004 10:58 AM To: Histonetliste Subject: Re: [Histonet] Formalin fix---then freeze? Would this method be adequate for lung to perform Sudan-stain on it? That opens the possibility to store the autopsy-tissue in formalin and do the staining later. Has anybody experience with this? Gudrun Lang ----- Original Message ----- From: "KMIN (Kathy Mink)" To: Sent: Tuesday, July 13, 2004 2:09 AM Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Tue Jul 13 10:36:55 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Lymph Node Fixative Message-ID: Does anyone have a homemade recipe for Lymph Node Fixative (Clearing)? Annette Featherstone Supervisor Anatomic Pathology Kaleida Health, Buffalo NY -----Original Message----- From: Carol Wilson [mailto:adelsyscarol@yahoo.com] Sent: Tuesday, July 13, 2004 11:14 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] looking to buy a Cytospin 3 or 4 Does anyone have a Cytospin 3 or 4 in good condition that they are interested in selling, and if so do you have an asking price? Thank you, Carol Wilson, H.T. (A.S.C.P.) . __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From minkk <@t> zgi.com Tue Jul 13 11:35:26 2004 From: minkk <@t> zgi.com (KMIN (Kathy Mink)) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: Thank you all for the great response. What would we do without the histonet? You guys are wonderful. Kathy Mink -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Tuesday, July 13, 2004 10:31 AM To: KMIN (Kathy Mink) Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fix---then freeze? Hi Kathy: This method is very do-able, is common and does make sense; you get the benefits of fixation (better morphology) and benefits of frozen sections (fat stains, immuno stains). I would suggest throwing in a cryoprotection step (20-30% sucrose overnight in the refrigerator) after washing out the formalin and, of course, rapid freezing to avoid ice crystal artifacts. Geoff KMIN (Kathy Mink) wrote: >Hi, > >I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! > >Kathy Mink > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From NSEARCY <@t> swmail.sw.org Tue Jul 13 11:42:29 2004 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Microscope Armrests Message-ID: <04Jul13.114240cdt.119093@healthcare2.sw.org> Anyone ever hear of "gel" arm rests? Any manufacturers besides Marketlab? Looking for a pathologist---- Thanks From joseph-galbraith <@t> uiowa.edu Tue Jul 13 11:42:49 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] cd-14 on paraffin, lyme-borreliose-antibody Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403005B22@uihc-mail1.uihc.uiowa.edu> Gudrun: Novocastra CD14 (NCL-CD14-223 or NCL-L-CD14-223), not certain about protocols specific for the Nexes. As for Lyme disease Ab's see Biocompare.com, search the site for 'lyme' then select 'Antibodies' for a list - notable manufacturers are Biodesign International, Fitzgerald Industries, US Biological, ViroStat Inc, etc. Never worked with a lyme disease Ab personally so no clue on protocols. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun Lang Sent: Tuesday, July 13, 2004 10:15 AM To: Histonetliste Subject: [Histonet] cd-14 on paraffin, lyme-borreliose-antibody Hi histonetters I am posting this question for my workmate. She is looking for an CD14-antibody on formalin fixed, paraffin-embed tissue, that is compatible for Ventana-Nexes. Any hints are welcome. ii. Is there an IHC-antibody for lyme-borreliose (borrelia burgdorferi) available (FFPE tissue, especially on skin) thanks in advance Gudrun Lang Akh Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From convmcm <@t> cc.usu.edu Tue Jul 13 12:01:13 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? In-Reply-To: Message-ID: <000001c468fb$018a7e30$4a737b81@Cygnus> I was wondering if you could do IFA staining on formalin fixed frozen sections. If antigen retrieval is necessary in PPFE, would that same antigen require retrieval for the IFA? It seems logical to me that it does, but I wonder if anyone has done this before. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KMIN (Kathy Mink) Sent: Tuesday, July 13, 2004 9:35 AM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin fix---then freeze? Thank you all for the great response. What would we do without the histonet? You guys are wonderful. Kathy Mink -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Tuesday, July 13, 2004 10:31 AM To: KMIN (Kathy Mink) Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin fix---then freeze? Hi Kathy: This method is very do-able, is common and does make sense; you get the benefits of fixation (better morphology) and benefits of frozen sections (fat stains, immuno stains). I would suggest throwing in a cryoprotection step (20-30% sucrose overnight in the refrigerator) after washing out the formalin and, of course, rapid freezing to avoid ice crystal artifacts. Geoff KMIN (Kathy Mink) wrote: >Hi, > >I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! > >Kathy Mink > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Tue Jul 13 12:03:25 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3B72@exchsrv01.barrynet.barry.edu> This used to be standard operating procedure for studies of phosphatases and cholinesterase. Several such protocols are in the 2nd and 3rd editions of Pearse's HISTOCHEMISTRY: THEORETICAL AND APPLIED. (2nd and 3rd editions are available used. The 4th edition is unobtainable.) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KMIN (Kathy Mink) Sent: Monday, July 12, 2004 8:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From terribraud <@t> msn.com Tue Jul 13 12:08:07 2004 From: terribraud <@t> msn.com (TERRI BRAUD) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] Re: Rickettsi Control Message-ID: From: Martha Ward To: Subject: [Histonet] Rick antibody Sent: Tuesday, July 13, 2004 8:19 AM I am going to post this question again since I got no response last week. I am looking for an alternative source for the R. ricksettsi antibody. We used to get it from the CDC but I understand from a colleague that it is not available from them. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center Martha- Could you please let me know where you get your Rickettsi control? [1]Terribraud@msn.com References 1. mailto:Terribraud@msn.com From PVilches <@t> nctr.fda.gov Tue Jul 13 12:37:30 2004 From: PVilches <@t> nctr.fda.gov (Vilches, Beth*) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] unsubscribe Message-ID: From funderwood <@t> mcohio.org Tue Jul 13 13:20:14 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:43 2005 Subject: [BULK] - [Histonet] Microscope Armrests Message-ID: I haven't tried this, but how about using the seat covers for bike seats that are gel filled? Fred >>> "Nita Searcy" 07/13/04 12:42PM >>> Anyone ever hear of "gel" arm rests? Any manufacturers besides Marketlab? Looking for a pathologist---- Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From swaram <@t> myrealbox.com Tue Jul 13 13:20:55 2004 From: swaram <@t> myrealbox.com (Swaram) Date: Fri Sep 16 15:23:43 2005 Subject: [Histonet] MART-1/Melan A from LabVision or Neomarkers Message-ID: <40F42807.3040903@myrealbox.com> Histonetters, I have been trying to use MART-1/Melan-A epitope specific Rabbit Ab on paraffin sections for flourescence based IHC. However, I see that the Ab stains quite differently from Pan Melanoma markers or even monoclonal Mouse Melan-A. I use pressure cooking for 15 min after boiling the water for my antigen retrieval. I have tried various conc. of primary Ab and I tried varying the conc. of Alexa F(ab)2 secondary conjugate. Does anyone have a better experience with this particular Ab from LabVision or Novocastra? From erskine.husbands <@t> gov.ky Tue Jul 13 11:04:53 2004 From: erskine.husbands <@t> gov.ky (Husbands, Erskine) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Plant section Message-ID: <591BFA887631DD4E9F00C0DEC7AAB2AC820419@SVR3_HS.hsa.ky> Is there anyone in Histonet land who can provide information on how to prepare and cut section on plant tissue. That information will be greatly appreciated. Thanking all in anticipation. From mayhew <@t> vaxxine.com Tue Jul 13 14:18:56 2004 From: mayhew <@t> vaxxine.com (D. Mayhew) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Automatic Coverslippers Message-ID: <002301c4690e$3efdde20$c6e805d1@dave> Hi Histonet, Does anyone use the combination of Histoclear and Permount for coverslipping with an automatic coverslipper? If so, could you please let me know which coverslipper you use and if you are satisfied with it. Thanks, Lee Mayhew MLT Niagara Health System St. Catharines, Ontario From maria <@t> ski.org Tue Jul 13 14:55:41 2004 From: maria <@t> ski.org (Maria Mejia) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] voltage sensitive dyes Message-ID: <40F43E3D.5070409@ski.org> Hello Histonet! I'm searching to talk with those individual/s who use voltage sensitive dyes for low biological noise probe for high-speed neural imaging of electrical signals in-vivo. I would greatly appreciate any information you can provide in the dye preparation using the blue dye labeled RH. I look forward to hearing from you!! regards Maria Mejia Smith-Kettlewell Eye Res. Inst. San Francisco, CA 94115 Email: maria@ski.org Phone: (415)-345-2185/2165 From algranth <@t> u.arizona.edu Tue Jul 13 15:12:16 2004 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Plant section In-Reply-To: <591BFA887631DD4E9F00C0DEC7AAB2AC820419@SVR3_HS.hsa.ky> Message-ID: <4.3.2.7.2.20040713130941.00c126a0@algranth.inbox.email.arizona.edu> There is a book on preparation of plant tissue for sectioning, Plant Microtechnique and Microscopy by Steve Ruzin that is very good. Steve is also very helpful if you contact him. I believe Amazon has the book. Andi Grantham At 11:04 AM 7/13/2004 -0500, Husbands, Erskine wrote: > Is there anyone in Histonet land who can provide information on how to >prepare and cut section on plant tissue. That information will be greatly >appreciated. > > Thanking all in anticipation. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From dlcowie <@t> prodigy.net Tue Jul 13 15:51:41 2004 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] radioactive tissue disposal Message-ID: <20040713205141.34433.qmail@web81003.mail.yahoo.com> hi netters, Does anyone know of any rules and reg's - CLIA, OSHA etc covering the safe handling and disposal of radioactive sentinel lymph nodes. All I have been able to find are CAP reccomendations. I am writing a policy to cover the handling, storage and disposal of specimens where the patient has been injected with technetium prior to surgery. The half life is 6 hours and they are safe to dispose of after 60 hours. Any suggestions on where to get references would be appreciated. Thanks, Dawn Cowie HT Pensacola Pathologists. From TJJ <@t> Stowers-Institute.org Tue Jul 13 16:08:51 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] BrdU Indirect IF staining Message-ID: We are attempting to do some indirect immunofluorescent double staining with anti-BrdU antibody in mouse tissues and various other antibodies. One of the problems we are having is denaturing the DNA for our BrdU staining. We obtain a strong, but somewhat non-specific signal with DNAse digestion. HCl pretreatment renders the nuclei unstainable by DAPI. We have had no luck in using HIER for denaturing (electric pressure cooker method). I suspect with most of our double stains with BrdU, we will be required to do the other antibody first, then denature and incubate with anti-BrdU, then use the fluorescent-labeled secondaries for detection. Can someone give me some suggestions as to how to optimize our pretreatment regimen without loss of nuclear integrity for immunofluorescence? What are you doing for BrdU immunostaining out there that's working, esp. using fluorescent-labeled secondaries? Has anybody successfully used the Xenon antibody labeling kit for direct labeling primary antibodies, and obtained good results in tissue IHC? Thanks for your help! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From int09018 <@t> alphahunt.com Tue Jul 13 16:30:27 2004 From: int09018 <@t> alphahunt.com (HCS) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] looking for LDL receptor antibody Message-ID: <000701c46920$9dff9820$6501a8c0@hp> Does anyone have access to LDL antibodies for "paraffin" sections. I can find some for frozens but would like to do the IHC on paraffin sections if possible. Any suggestions as to where to look? Anyone with a protocol? Can it be done? Thanks in advance. LeRoy Brown HT(ASCP) HTL Histology Consultation Services www.histocs.com Everson, WA 98247 360-966-7300 From lizchlipala <@t> premierhistology.com Tue Jul 13 16:42:11 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] pricing for cytospin preps Message-ID: <000201c46922$44ba6f90$74d48a80@LIZ> Hello everyone Would anyone be willing to share their pricing with me for cytospin preparations in the research setting. How much do you charge to prepare one cytospin slide. And then do you charge the same for multiple slides from the same specimen. Thanks in advance. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From lpwenk <@t> sbcglobal.net Tue Jul 13 17:41:57 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] radioactive tissue disposal References: <20040713205141.34433.qmail@web81003.mail.yahoo.com> Message-ID: <005201c4692a$9b337440$e8a4ff44@domainnotset.invalid> Do you have a radiation safety officer at your facility? They would be associated with the Safety Department or with one of the "radiation" departments, such as nuclear medicine or brachitherapy. They have a lot of training in this, lots of reference books, know all the radiation regulations (handling, disposal, etc.), and/or can take the radiation readings for you so you have documentation. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Dawn Cowie" To: "histonet" Sent: Tuesday, July 13, 2004 4:51 PM Subject: [Histonet] radioactive tissue disposal > hi netters, > Does anyone know of any rules and reg's - CLIA, OSHA etc covering the safe handling and disposal of radioactive sentinel lymph nodes. All I have been able to find are CAP reccomendations. I am writing a policy to cover the handling, storage and disposal of specimens where the patient has been injected with technetium prior to surgery. The half life is 6 hours and they are safe to dispose of after 60 hours. Any suggestions on where to get references would be appreciated. > Thanks, > Dawn Cowie HT > Pensacola Pathologists. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Krat18 <@t> aol.com Tue Jul 13 18:17:19 2004 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Cost per test, Ventana vs others Message-ID: I have two questions: I sent this one earlier today about whether anyone knows of any IHC system other than Ventana's that can stain for HPV's, Her-2/Neu's, ER's, and PR's (all of the above). And my other question is, does anyone have an accurate cost per test for Ventana's immunos vs immunos from other manufacturers? Karen_Raterman@ssmhc.com krat18@aol.com From AnthonyH <@t> chw.edu.au Tue Jul 13 19:00:52 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E20B@simba.kids> Gudrun, Yes, we have often had to resort to this. Rinse the tissue in water 30min. Infiltrate in 30% OCT (room temp)or sucrose untill the tissue sinks. Pat the tiisue to remove excess fluid Embed in OCT, freeze and section Use sticky slides to help adherence. I usually air dry for 15-30 min to aid adhesion Stain for Fats etc as required. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Wednesday, 14 July 2004 12:58 AM To: Histonetliste Subject: Re: [Histonet] Formalin fix---then freeze? Would this method be adequate for lung to perform Sudan-stain on it? That opens the possibility to store the autopsy-tissue in formalin and do the staining later. Has anybody experience with this? Gudrun Lang ----- Original Message ----- From: "KMIN (Kathy Mink)" To: Sent: Tuesday, July 13, 2004 2:09 AM Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From RSRICHMOND <@t> aol.com Tue Jul 13 20:53:01 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Formalin fix---then freeze? Message-ID: <158.39a62d23.2e25ebfd@aol.com> One of the secrets of cutting frozen sections from formalin fixed tissue: Albuminize the slides, rather thickly, to make the sections adhere. - The request is quite reasonable. Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From RSRICHMOND <@t> aol.com Tue Jul 13 21:33:54 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Control block for leprosy Message-ID: <12e.464676f3.2e25f592@aol.com> Pat Willis asked: >>Is there anybody out there with a known supply of leprosy control blocks? Preferably UK based. We'd appreciate it if you could contact us with details.<< I don't know why Mycobacterium leprae controls are so hard to get. There's an animal model for Hansen's disease - when the American nine banded armadillo (Dasypus novemcinctus) gets leprosy - naturally or experimentally - their livers turn into practically a pure culture of the bug. Why aren't blocks of leprous armadillo liver made available as controls? (You do need a lepra bacilli control if that's what is to be stained for - its acid fast properties are different from those of Myco tuberculosis. - Does anyone know if MAI has the same staining properties as Myco tuberculosis?) Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From rentonlf <@t> bru.wits.ac.za Wed Jul 14 02:14:56 2004 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] OT then freeze? Message-ID: <1089789296.8e201a20rentonlf@bru.wits.ac.za> Talk about showing age: I used to use a freezing microtome for in-theatre frozen sections. It was housed in a little cubicle just off the recovery room. What those poor people thought of the deafening WHOOSH of the Co2 I never found out! Regards -----Original Message----- From: "Houston, Ronnie" To: "'KMIN (Kathy Mink)'" , histonet@lists.utsouthwestern.edu Date: Tue, 13 Jul 2004 09:58:37 -0400 Subject: RE: [Histonet] Formalin fix---then freeze? Absolutely; although there is a very real possibility that the tissue will float off the slide (no matter what type of slide you use) if cutting with a cryostat. Wash the tissue well before freezing. The microtome of choice for sectioning frozen fixed tissue is a freezing microtome, although there aren't many about any more. What a shame!....now that is an art and experience that younger histotechs are losing out on....I remember the days of free-floating cerebellum sections................showing my age now. Ronnie Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 2877972 (804) 2877906 - fax ronnie_houston@bshsi.com -----Original Message----- From: KMIN (Kathy Mink) [mailto:minkk@zgi.com] Sent: Monday, July 12, 2004 8:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin fix---then freeze? Hi, I have been asked an unusual (stupid?) question and hope someone can help. Is it possible to formalin fix a tissue and then put it in OCT and freeze it to cut frozen sections? I know it doesn't make much sense but apparently a contract we have was worded this way (obviously not proof read by the pathologist) and now I need to treat the tissue this way if possible. Help! Kathy Mink _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From rentonlf <@t> bru.wits.ac.za Wed Jul 14 02:26:07 2004 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] eosin fading Message-ID: <1089789967.8e201a20rentonlf@bru.wits.ac.za> One of the reasons that H&E slides fade is leaving them out in the light. Both fluorescent (some of the lights here are on 24/7) and natural light fade slides to some extent. -----Original Message----- From: Marshall To: histonet@lists.utsouthwestern.edu Date: Tue, 13 Jul 2004 14:06:17 +0200 Subject: [Histonet] eosin fading Hi all, Would like an opinion on what causes eosin to fade while we are on the fading of hematoxylin debate. I find that when I stain a batch of slides 6 months to a year later some of these slides are a lot paler as regards eosin. I then restain and the slide is fine again. I use a eosin/phloxine mixture. Would using an alcoholic eosin mixture improve this? I am using entellan as mounting medium. Thanks sharon marshall e-mail : marshall@cormack.uct.ac.za Dept. Human Biology University of Cape Town South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From daniel.eberhard <@t> uni-bielefeld.de Wed Jul 14 02:49:11 2004 From: daniel.eberhard <@t> uni-bielefeld.de (DANIEL EBERHARD) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] related question; but FREEZE- Formalin fix---then freeze? In-Reply-To: <4C051EAE581BB646BF53A749A73FBA2D1F3B72@exchsrv01.barrynet.barry.edu> References: <4C051EAE581BB646BF53A749A73FBA2D1F3B72@exchsrv01.barrynet.barry.edu> Message-ID: <40F4E577.8090000@UNI-BIELEFELD.DE> Dear Histonetters, I have a succeeding question to the "formalin-freeze" topic. Accidentally some mouse tissues (with cytosolic and highly soluble GFP), which I routinely pre-fix in pFA, wash and freeze, were frozen without pre-fixation (stored at -80?C). Now, I would like to *thaw *the tissue, *fix it* (pFA) and *freeze* it again, but of course thawing (e.g. crystal formation) might ruin cells and membranes, so that the GFP might pour out. Thus, I?m searching for some tips now to manage this? Has anyone done sth. like this before? "Post-pFA-fixation" of sections is unfortunately impossible. Thanks for any tips and tricks. Daniel. From apqap <@t> rcpaqap.com.au Tue Jul 13 17:12:41 2004 From: apqap <@t> rcpaqap.com.au (RCPA QAP Pty Ltd) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Storage of H&E slides Message-ID: <5.0.0.25.2.20040714080547.0323cce0@mail.netcore.com.au> Dear all I am looking for a reference to support the statement that H&E slides are stable (ie can be stored without losing the quality of the staining). Does anyone know of one? Margaret RCPA Quality Assurance Programs Pty Ltd Anatomical Pathology Unit 3, 15-21 Huntingdale Rd Burwood Vic 3125 Australia email apqap@rcpaqap.com.au web www.rcpaqap.com.au From Barry.R.Rittman <@t> uth.tmc.edu Wed Jul 14 08:37:06 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Storage of H&E slides Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0D82F78@UTHEVS3.mail.uthouston.edu> Margaret I do not know of any reference related specifically to this. It would be difficult because, as has been pointed out, fading may depend on the type of hematoxylin, type of eosin procedures, clearing agents, mountants and storage conditions and even small differences may alter the end product. The fact that there are slides from the late 1800s that are still stained with dyes such as carmine and some with hematoxylin attests to the fact that hematoxylin at least can survive well. Eosin seems a bit more variable. There is considerable variation between slides that have been treated identically. I have slide that I prepared 40 years ago that are still well stained with H and E and others prepared at the same time using the same processing and staining that have faded. I believe that the greatest concern with storage should be the adhesion of cover glasses. Once air creeps under the cover glass the sections fade. Hope this helps. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RCPA QAP Pty Ltd Sent: Tuesday, July 13, 2004 5:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of H&E slides Dear all I am looking for a reference to support the statement that H&E slides are stable (ie can be stored without losing the quality of the staining). Does anyone know of one? Margaret RCPA Quality Assurance Programs Pty Ltd Anatomical Pathology Unit 3, 15-21 Huntingdale Rd Burwood Vic 3125 Australia email apqap@rcpaqap.com.au web www.rcpaqap.com.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ECSIMON <@t> NORTHERNHEALTH.ORG Wed Jul 14 09:42:32 2004 From: ECSIMON <@t> NORTHERNHEALTH.ORG (Ellen Clausen-Simon) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] (no subject) Message-ID: From ftulenko06 <@t> jcu.edu Wed Jul 14 10:26:54 2004 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] histonet Message-ID: <2f5bddbc.5fd35651.81a7200@mirapoint.jcu.edu> I was wondering if anybody had any experience using Gomori 1-2-3 fixative. How long is required for fixation? Thanks From juan.gutierrez <@t> christushealth.org Wed Jul 14 10:38:32 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] looking for LDL receptor antibody Message-ID: If by LDL you are referring to low density lipoproteins, my guess is no. Lipids are usually dissolved during processing by xylene. I think you are stuck with frozens. Then again I might be wrong, good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: HCS [mailto:int09018@alphahunt.com] Sent: Tuesday, July 13, 2004 4:30 PM To: Histonet Subject: [Histonet] looking for LDL receptor antibody Does anyone have access to LDL antibodies for "paraffin" sections. I can find some for frozens but would like to do the IHC on paraffin sections if possible. Any suggestions as to where to look? Anyone with a protocol? Can it be done? Thanks in advance. LeRoy Brown HT(ASCP) HTL Histology Consultation Services www.histocs.com Everson, WA 98247 360-966-7300 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From int09018 <@t> alphahunt.com Wed Jul 14 11:31:09 2004 From: int09018 <@t> alphahunt.com (HCS) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] thanks to responders to my LDL question Message-ID: <000701c469bf$f8a41770$6501a8c0@hp> Thanks for your input on LDL antibodies. I need to keep looking; more specifically I am looking for LDL receptor antibodies for use on paraffin sections. I have found some companies that say their antibodies would "probably work" but have not been tested on paraffin. Some of the responses follow: see some ldl receptor abs at: http://www.researchd.com/miscabs/lipoabs.htm Go to this link It is very informational. http://www.antibodyassay.com/testinfo/oxidized_ldl_antibodies.htm Or this web site http://www.researchd.com/miscabs/pro61080.htm Chemicon International has a few LDL abs. Check with their tech people to see if any have been tested on paraffin. They offer a 50% rebate on any antibody that may not have been tested in a certain application. Sincerely LeRoy Brown HT(ASCP) HTL Histology Consultation Services Everson, WA 98247 www.histocs.com From kjgavel <@t> nsac.ns.ca Wed Jul 14 11:33:39 2004 From: kjgavel <@t> nsac.ns.ca (Krista J Gavel) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Cutting Fat for IHC Message-ID: <1089822819.9fe724c0kjgavel@nsac.ns.ca> I am experiencing difficulties trying to section mouse abdominal adipose and mink adipose in the cryostat. The tissues were frozen at -80C in chunks. When attempting to make sections, the tissue sticks to itself, and clumps up. I have tried fixing in paraformaldehyde and using histofreeze, but the tissue is still too soft. The cryostat is at its lower limit, about -32C. Is there anything I can do? From Linresearch <@t> aol.com Tue Jul 13 18:25:11 2004 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Cholesterol Stains Message-ID: <1df.2539f1d5.2e25c957@aol.com> Hi, I need to do Cholesterol staining in FFPE tissues. Any help would be appreciated. Lin From mab70 <@t> medschl.cam.ac.uk Tue Jul 13 03:44:18 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Control block for leprosy[Scanned] Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111378@mius2.medlan.cam.ac.uk> Pat, When I worked in my last job, I had dealings with Peterborough Hospital who have a tissue bank set up. I don't know if they would have anything as specialised as leprosy controls but they have set up a very professional business offering blocks, staining and tissue to research organisations and all the legal work is covered too. If I remember correctly, the guy to contact there is Neil Gray. If they are able to supply you, you will need to sign some legal documents which are for patient protection purposes. I hope this helps. Regards Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Pat Willis [mailto:Pat.Willis@mail.bhrv.nwest.nhs.uk] Sent: Monday, July 12, 2004 4:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Control block for leprosy[Scanned] Is there anybody out there with a known supply of leprosy control blocks? Preferably UK based. We'd appreciate it if you could contact us with details. Thanks in advance, Pat _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From je22r <@t> udcf.gla.ac.uk Wed Jul 14 12:31:48 2004 From: je22r <@t> udcf.gla.ac.uk (Julia Edgar) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] BrdU Indirect IF staining Message-ID: <000c01c469c8$7173b2c0$40e9d182@vet> Dear Teri Here's a protocol I have used in the past. However, I don't know if it inhibits DAPI staining (but see below). For cryostat sections of tissue perfused with paraformaldehyde or similar, stain your sections with the other antibody (primary and secondary) then fix sections in 50% acetic acid/50% ethanol for 10 minutes and wash thoroughly in PBS. Treat sections with 50% HCl/1% Triton-X 100 for 10 minutes, room temperature to denature the DNA. Wash thoroughly in PBS then bock and apply the anti-BrdU antibody for 2 hours, room temperature, in blocking solution with 0.2% Triton-X 100. Wash and apply secondary antibody. On cells, I have used 0.07M NaOH (made fresh) to denature the DNA. This does NOT inhibit DAPI staining, but I have not used this method on tissue sections. Julia Julia Edgar BSc (Hons), PhD University of Glasgow Veterinary School From jengirl1014 <@t> yahoo.com Wed Jul 14 12:40:10 2004 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Eosin Staining Message-ID: <20040714174010.77105.qmail@web60609.mail.yahoo.com> I recently did some H&E staining for a PI and they said that the Eosin was too pink. She was told that I could remove the cover slip using xylene and could reduce the Eosin that way so I wouldn't have to cut any more slides. Would anyone know how to do this without hurting the tissue? Thanks, Jen --------------------------------- Do you Yahoo!? Yahoo! Mail Address AutoComplete - You start. We finish. From jcline <@t> wchsys.org Wed Jul 14 12:42:35 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Receiving mail Message-ID: <000001c469c9$f4947710$1d2a14ac@wchsys.org> I am not receiving Histonet messages. Could my IS department consider this web site a problem, that would be the only reason I have stopped getting messages? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From gcallis <@t> montana.edu Wed Jul 14 13:51:00 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] removing Eosin Staining In-Reply-To: <20040714174010.77105.qmail@web60609.mail.yahoo.com> References: <20040714174010.77105.qmail@web60609.mail.yahoo.com> Message-ID: <6.0.0.22.0.20040714124257.01b2dda0@gemini.msu.montana.edu> After taking off coverslip with xylene, rehydrate the slide - rinsing in 70% or even going back to tap water rinse will take out some eosin. Eosin is soluble in water and alcohol, if you take out too much eosin, just restain, preferably with a 70% alcohol rinse BEFORE the eosin, then rinse with 95% afterwards to differentiate the color. Overstaining with eosin is a common problem - you may have to adjust your eosin staining time (less) for her next bunch of slides. The famous Culling was adamant against too much eosin staining and warned people to beware - make it light, with 5 to 6 different shades of red to pink (different tissue components will have a different red or tinctorial level) - and he felt most pathologists and other people were looking H&E with eosin much too red. From j_gorenstein <@t> yahoo.com Wed Jul 14 14:45:13 2004 From: j_gorenstein <@t> yahoo.com (Juile Gorenstein) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] processing of whole late stage (E18) mouse embryos (frozen and paraffin) Message-ID: <20040714194513.42218.qmail@web42004.mail.yahoo.com> Hello everyone, I have been trying to process and section late stage embryos both for paraffin and frozen sections. I would like to be able to do histology, ISH and IHC on paraffin sections, as well as LacZ staining on frozen sections (saggital). I have heard that I need to fix in PFA (NBF) to be able to do ISH and IHC. Is this necessary? Has anyone ever tried to do ISH and IHC on Bouin's or Carnoy's fixed tissue? Could anyone suggest what is the best way to fix these guys without mutilation (ie separation of head from the body or making large incisions)? I also had problems dehydrating the tissue prior to paraffin embedding, even using tissue processor. I think it's dehydration step, anyways. The tissue comes out soft and whitish in places. I have tried to reprocess that tissue (manually and with a processor deparaffinize, then reprocess again using the tissue processor). This makes the tissue overly dehydrated and crumbly when attempted to section. Could anyone suggest an appropriate program to use the first time? Also, I have been having problems cutting frozen sections (saggital of whole E18). The tissue separates from OCT, and some of the tissues crumbles, depending on temperatures I use on the cryostat. I have tried to use -10oC sample temp, but then OCT has problems being cut. Any suggestions? Is there anything I can do to make OCT stick better to the skin? Are there temperatures that most fit for various types of tissues to be attempted to cut at once? I am sorry this request has become so large. Any suggestions will be really appreciated. Thank you all, Julie Julie Gorenstein Novartis, Cambridge, MA --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. From SJones <@t> cvm.tamu.edu Wed Jul 14 15:06:45 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] processing of whole late stage (E18) mouse embryos(frozen and paraffin) Message-ID: Hi Juile, The tissue sounds like it is not being dehydrated enough. What times are you using on the processor? What are the dimensions of the embryos? What clearing agent are you using on the processor? What processor do you have? Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> Juile Gorenstein 7/14/2004 2:45:13 PM >>> Hello everyone, I have been trying to process and section late stage embryos both for paraffin and frozen sections. I would like to be able to do histology, ISH and IHC on paraffin sections, as well as LacZ staining on frozen sections (saggital). I have heard that I need to fix in PFA (NBF) to be able to do ISH and IHC. Is this necessary? Has anyone ever tried to do ISH and IHC on Bouin's or Carnoy's fixed tissue? Could anyone suggest what is the best way to fix these guys without mutilation (ie separation of head from the body or making large incisions)? I also had problems dehydrating the tissue prior to paraffin embedding, even using tissue processor. I think it's dehydration step, anyways. The tissue comes out soft and whitish in places. I have tried to reprocess that tissue (manually and with a processor deparaffinize, then reprocess again using the tissue processor). This makes the tissue overly dehydrated and crumbly when attempted to section. Could anyone suggest an appropriate program to use the first time? Also, I have been having problems cutting frozen sections (saggital of whole E18). The tissue separates from OCT, and some of the tissues crumbles, depending on temperatures I use on the cryostat. I have tried to use -10oC sample temp, but then OCT has problems being cut. Any suggestions? Is there anything I can do to make OCT stick better to the skin? Are there temperatures that most fit for various types of tissues to be attempted to cut at once? I am sorry this request has become so large. Any suggestions will be really appreciated. Thank you all, Julie Julie Gorenstein Novartis, Cambridge, MA --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Wed Jul 14 15:12:23 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] no messages since 7-9 from list Message-ID: Just checking to see why I have not gotten any messages since Friday. Patsy From Kim.Kolman <@t> med.va.gov Wed Jul 14 15:22:49 2004 From: Kim.Kolman <@t> med.va.gov (Kim.Kolman@med.va.gov) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] unsubscribe Message-ID: From northma <@t> ohsu.edu Wed Jul 14 16:02:00 2004 From: northma <@t> ohsu.edu (Mary North) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Cutting Fat for IHC Message-ID: You might want to try chilling the block face and the knife with liquid nitrogen, if you have it available. By clamping a gauze pad in the clamps of a hemostat or other such device, you can dip into liquid nitrogen and then press the cold pad where you need it. Excess liquid nitrogen will drip off into the cryostat. Quite often, the muscle specimens I section are fatty and this technique works. The first section off may contain excessive frost so the next section is usually just right. Mary North, HT(ASCP), HTL Neuromuscular Lab Oregon Health and Science University Portland, OR >>> "Krista J Gavel" 7/14/2004 9:33:39 AM >>> I am experiencing difficulties trying to section mouse abdominal adipose and mink adipose in the cryostat. The tissues were frozen at -80C in chunks. When attempting to make sections, the tissue sticks to itself, and clumps up. I have tried fixing in paraformaldehyde and using histofreeze, but the tissue is still too soft. The cryostat is at its lower limit, about -32C. Is there anything I can do? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From spoulos <@t> saa.ars.usda.gov Wed Jul 14 16:12:07 2004 From: spoulos <@t> saa.ars.usda.gov (Sylvia Poulos) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Cutting Fat for IHC Message-ID: Try fixing your tissue in Bouin's fixative if you have the option. The tissue becomes a bit more rigid and easier to cut and we've never had a problem doing immunos or histology on it. Good luck, Sylvia Sylvia P. Poulos USDA-ARS-Animal Physiology Research Unit Athens, GA 30605 706-583-8279 706-542-0399 (fax) From GoodwinD <@t> pahosp.com Wed Jul 14 16:49:44 2004 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Threshold standard for labeling errors Message-ID: <992899E9EC268548AB8DDE246AF8847302B7163E@PAHEX01.uphs.upenn.edu> Greetings, Histonetters. I understand that 0% is the optimal threshold, however I'd like to get a consensus of the standard that clinical histo labs have set for slide/block labeling errors. Thanks, Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Philadelphia, PA From dcrippen <@t> buckinstitute.org Wed Jul 14 17:39:30 2004 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] IHF with blue conjugated secondaries Message-ID: <4AA34A707932424EBE2D973764D226A9FAC30D@inverness.buckcenter.org> Dear All, I have an investigator who is in need of triple fluorescence labeling on mouse brain tissue. The green fluorophore is injected and the request is that we double label for two additional antigens. Our problem is that we cannot find a blue conjugated secondary that works at all. We've purchased AMCA Dn x Rb (Jackson) and 350 Dn x Gt (Molecular Probes) and have tried them at every possible working concentration recommended on the spec sheets. We've included GFAP positive controls with Cy3 secondary (this works beautifully) and we've tried using alternate tissue to eliminate the possibility that it's our particular sample. We've also tried varying the blocking method and several different protocols. Considering the confines of our microscope set-up, we must use a blue and a red conjugated secondary. Additionally, one of our primary antibodies is made in goat, so both our secondaries must be made in donkey. Any suggestions as to vendors, protocols or particular references from the literature are VERY welcome!!! Many many thanks in advance!! Danielle Crippen Morphology Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org From d.gregg <@t> juno.com Wed Jul 14 21:20:43 2004 From: d.gregg <@t> juno.com (Douglas A Gregg) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] 40F4E577.8090000@UNI-BIELEFELD.DE Message-ID: <20040714.222044.328.1.d.gregg@juno.com> Message: 22 Date: Wed, 14 Jul 2004 09:49:11 +0200 From: DANIEL EBERHARD Subject: [Histonet] related question; but FREEZE- Formalin fix---then freeze? To: histonet@lists.utsouthwestern.edu Message-ID: <40F4E577.8090000@UNI-BIELEFELD.DE> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Dear Histonetters, I have a succeeding question to the "formalin-freeze" topic. Accidentally some mouse tissues (with cytosolic and highly soluble GFP), which I routinely pre-fix in pFA, wash and freeze, were frozen without pre-fixation (stored at -800C). Now, I would like to *thaw *the tissue, *fix it* (pFA) and *freeze* it again, but of course thawing (e.g. crystal formation) might ruin cells and membranes, so that the GFP might pour out. Thus, I4m searching for some tips now to manage this? Has anyone done sth. like this before? "Post-pFA-fixation" of sections is unfortunately impossible. Thanks for any tips and tricks. Daniel. Daniel, I think I can help you with this one. I think I am getting to the age that I have made most of the mistakes or had to deal with them. I once had a bull dog submitted for a post mortem with a threat of litgation because it died at the groomer. These dogs can die from any kind of excitement because they have so much redundant pharyngeal tissue to choke on. The bad part was the dog was quick frozen on dry ice before submission. It was going to take a couple days to thaw it out. I got tired of waiting and figured it would be a gross diagnosis anyway so I posted it when it was crunchy and I could just move the limbs. I put crunchy tissues in formalin figuring the freeze artifact would be terrrible in any case. I have gotten tissue in the mail in the winter and tissue frozen in formalin is the worst. To my surprise the tissues looked great. Now I don't hesitate to drop frozen tissue into formalin and let it thaw and fix at the same time. I also work with GFP but use formalin fixed paraffin embedded tissue and a MAb against GFP after Dako antigen retrieval in the autoclave. I use Vector ABC alk phos and Vector red. The results are spectacular bright red staining and hematox counterstain. I highly recommend it. Forget using FA. The signal was not that great and it would fade as well by FA. These slides are permanent. If someone really wants fluorescence, the Vector red lights up great with a Texas red filter. If you are doing confocal, just switch the colors to green if you like green. The signal is even better than the red staining using white light. I have found weakly stained cells that I could not see any other way. I think it is the way to go. Good luck. Doug Gregg Veterinary pathologist Plum Island Animal Disease Center From katri <@t> cogeco.ca Wed Jul 14 21:42:04 2004 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Eosin Staining References: <20040714174010.77105.qmail@web60609.mail.yahoo.com> Message-ID: <001901c46a15$50aa92f0$6a9a9618@Katri> Hi Jennifer, Remove the coverslip in xylene and bring the section back through one or two changes of xylene and absolute alcohol and then into 95% ethanol. Leave in ethanol until enough Eosin has come out of the section and then dehydrate, clear and mount. That should take care of it. Katri Katri Tuomala St.Joseph's Health Care Hamilton, Ontario, Canada ----- Original Message ----- From: "Jennifer Sipes" To: Sent: Wednesday, July 14, 2004 1:40 PM Subject: [Histonet] Eosin Staining > I recently did some H&E staining for a PI and they said that the Eosin was too pink. She was told that I could remove the cover slip using xylene and could reduce the Eosin that way so I wouldn't have to cut any more slides. Would anyone know how to do this without hurting the tissue? > > > Thanks, > > Jen > > > --------------------------------- > Do you Yahoo!? > Yahoo! Mail Address AutoComplete - You start. We finish. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- ---- From kdawwas <@t> hotmail.com Thu Jul 15 01:54:03 2004 From: kdawwas <@t> hotmail.com (Kamal Dawwas) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry staining machines Message-ID: I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ From jaimie_hk <@t> yahoo.co.uk Thu Jul 15 02:29:44 2004 From: jaimie_hk <@t> yahoo.co.uk (=?iso-8859-1?q?Jaimie=20Hoh?=) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Eosin Staining Message-ID: <20040715072944.75815.qmail@web25005.mail.ukl.yahoo.com> Hi Jennifer, This is what i normally do when I overstained with Eosin: After rehydrating the specimen with Xylene and then descending concentrations of ethanol; 1. if the eosin is aqueous base, then i'll let the slides for a few minutes in water until the intensity of the Eosin is correct. 2. If the Eosin is Alcoholic, then I?ll let the slides in 95% Ethanol for a few minutes until the Eosin fade to the intensity desired. Jaimie Research Officer Singapore Eye Research Institute ___________________________________________________________ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself http://uk.messenger.yahoo.com From swelam48 <@t> hotmail.com Thu Jul 15 05:46:49 2004 From: swelam48 <@t> hotmail.com (Wael Swelam) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Flk-1 immunostaining protocol Message-ID: Dear all, I have been trying immunostaining for Flk-1 using different protocoles (Autoclave treatment using citrate buffer OR Tris-EDTA), enzyme treatment (proteinase K 5ug/ml); for Flk-1 monoclonal antibody (Santa cruse) i used 1:50 conc using Envesion detecting system. but i don't have the strong specific signal. Can any one help Thanks Wael Swlam Ph.D student Niigata University,Japan From DDittus787 <@t> aol.com Thu Jul 15 08:18:52 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Threshold standard for labeling errors Message-ID: <1F67CC34.6660140E.0A1F969F@aol.com> Diana; we use a 3% threshold and track specimen labeling errors(o.r.,LDR, ER) then we track histotech,P.A. errors and pathologists errors (slide and block mislabeling, tissue issues,etc),we also track floaters, contaminents,etc and morgue issues as well. this way we have a pre-analytic, analytic and post analytic view. Hope this helps if you need any copies of what we do I would be glad to fax them to you. Dana P.S. so far we have stayed under 1% In a message dated 7/14/2004 5:49:44 PM Eastern Daylight Time, "Goodwin, Diana" writes: >Greetings, Histonetters. > >I understand that 0% is the optimal threshold, however I'd like to get a >consensus of the standard that clinical histo labs have set for slide/block >labeling errors. > >Thanks, > >Diana Goodwin >Supervisor, Anatomic Pathology >Pennsylvania Hospital >Philadelphia, PA ? ? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From juan.gutierrez <@t> christushealth.org Thu Jul 15 08:53:04 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry staining machines Message-ID: Ventana's Benchmark XT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sue.Kapoor <@t> uhsi.org Thu Jul 15 08:58:02 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] changing from high to low profile blades Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5612@khmcexch.uhsi.org> Hi all, I have a Reichert-Jung 2030 microtome that I recently changed the back plate on so that we can use low profile blades. We're expriencing alot of problems in sectioning now, finding it extremely difficult to get a ribbon. The sections just compress at the blade. I've tried adjusting the angle, (it had been at 3 micron), didn't seem to help....any suggestions??????!!!??? Thank you, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From Marion.Hiles <@t> north-bristol.swest.nhs.uk Thu Jul 15 09:03:45 2004 From: Marion.Hiles <@t> north-bristol.swest.nhs.uk (Marion Hiles) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry staining mach ines Message-ID: <2EE924DF60902943AC6E2EF35155451F19CD27@nbfexch03.north-bristol.nhs> We use the OPTIMAX PLUS machine manufactured by Biogenex and its brilliant. It is only a 40 slide capacity machine but I think there is a larger capacity one out there. Bob Quilty Neuropathology Dept Frenchay Hospital BRISTOL, UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GUTIERREZ, JUAN Sent: 15 July 2004 14:53 To: Kamal Dawwas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Ventana's Benchmark XT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorised. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. From portera203 <@t> yahoo.com Thu Jul 15 09:46:27 2004 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] endogenous alk phos block and HIER Message-ID: <20040715144627.64891.qmail@web40904.mail.yahoo.com> Just curious to know what everyone thinks about pretreatments for endogenous products and Heat induced retrieval methods. Do you do the blocking before the retrieval or after? I am always worried about effecting the pH treatment from the retrieval if i pretreat for endogenous after retrieval. On the other hand if I block first and then retrieve, am i re-opening what i just tried to get rid of while retrieving. I hope this makes sense to someone and opinions would be greatly appreciated. I know i will get lots of good information, as always - Thanks in advance for all the help. Amy S.Porter, HT(ASCP) Michigan State University Department of Physiology Division of Human Pathology College of Human Medicine portera203@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Mail is new and improved - Check it out! From gcallis <@t> montana.edu Thu Jul 15 09:52:02 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] changing from high to low profile blades In-Reply-To: <61E9F2400F53D5119CFC00508B44E33B019F5612@khmcexch.uhsi.org > References: <61E9F2400F53D5119CFC00508B44E33B019F5612@khmcexch.uhsi.org> Message-ID: <6.0.0.22.0.20040715084105.01b455e0@gemini.msu.montana.edu> Sorry, but 3 micron is NOT the angle, that is the section thickness. The angle adjustment is on the side of the knife holder usually marked with 0, 5, and 10 with 5 equal to 10 degrees on my Leica. If you take the bottom slash mark of holder and move it to back, so it equals around 6 aka 12 degrees or so, keep working with it, you should find the correct angle for your particular blade. Some blades are better than others - you can always try another source. Richard Allan Edge Rite are good, have tried some of Thermo Electrons aka Shandons with success and Dura Edge - get samples. With low profiles, depending on manufacturer of blades, you might have to increase the angle to around 12 degrees or even more - differing from high profile. I have never found any difference in sharpness between high and low profiles, but I have found stability differences. We use high profiles for cryo and regular microtomy in order to cut denser tissue without chatter and no compression. At 07:58 AM 7/15/2004, you wrote: >Hi all, > I have a Reichert-Jung 2030 microtome that I recently changed the back >plate on so that we can use low profile blades. We're expriencing alot of >problems in sectioning now, finding it extremely difficult to get a ribbon. >The sections just compress at the blade. I've tried adjusting the angle, (it >had been at 3 micron), didn't seem to help....any suggestions??????!!!??? > >Thank you, >Sue Kapoor, HT (ASCP) >Histology Coordinator >Kenosha Medical Center >Kenosha, WI >262-653-5570 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From brokeponi <@t> yahoo.com Thu Jul 15 09:54:56 2004 From: brokeponi <@t> yahoo.com (Christine Baker) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: <20040715145456.97644.qmail@web52409.mail.yahoo.com> I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. From Sue.Kapoor <@t> uhsi.org Thu Jul 15 09:57:47 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] changing from high to low profile blades Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5613@khmcexch.uhsi.org> my mistake...NOT 3 micron......3 degrees -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Thursday, July 15, 2004 9:52 AM To: Kapoor, Sue; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] changing from high to low profile blades Sorry, but 3 micron is NOT the angle, that is the section thickness. The angle adjustment is on the side of the knife holder usually marked with 0, 5, and 10 with 5 equal to 10 degrees on my Leica. If you take the bottom slash mark of holder and move it to back, so it equals around 6 aka 12 degrees or so, keep working with it, you should find the correct angle for your particular blade. Some blades are better than others - you can always try another source. Richard Allan Edge Rite are good, have tried some of Thermo Electrons aka Shandons with success and Dura Edge - get samples. With low profiles, depending on manufacturer of blades, you might have to increase the angle to around 12 degrees or even more - differing from high profile. I have never found any difference in sharpness between high and low profiles, but I have found stability differences. We use high profiles for cryo and regular microtomy in order to cut denser tissue without chatter and no compression. At 07:58 AM 7/15/2004, you wrote: >Hi all, > I have a Reichert-Jung 2030 microtome that I recently changed the back >plate on so that we can use low profile blades. We're expriencing alot of >problems in sectioning now, finding it extremely difficult to get a ribbon. >The sections just compress at the blade. I've tried adjusting the angle, (it >had been at 3 micron), didn't seem to help....any suggestions??????!!!??? > >Thank you, >Sue Kapoor, HT (ASCP) >Histology Coordinator >Kenosha Medical Center >Kenosha, WI >262-653-5570 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Jackie.O'Connor <@t> abbott.com Thu Jul 15 10:11:55 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Platelets in FFPE Message-ID: Does anyone have a tried and true method for demonstrating resting or activated platelets in FFPE? If not, how about frozen sections? Zinc fixed tissues? ANYTHING? I love you guys - Jackie O'Connor From JNocito <@t> Pathreflab.com Thu Jul 15 10:25:26 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry staining machines In-Reply-To: Message-ID: Okay, You heard it from me first. Ventana Benchmark XT. I now am the proud owner of 2 XTs. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GUTIERREZ, JUAN Sent: Thursday, July 15, 2004 8:53 AM To: Kamal Dawwas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Ventana's Benchmark XT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sue.Kapoor <@t> uhsi.org Thu Jul 15 10:27:43 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] high to low profile blades Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5614@khmcexch.uhsi.org> Thank you all for your answers, it appears to be the angle. I tried a 10 degree angle and it seems to be working now. Since it was set at 3 degrees with the high profile blades I was a little weary of trying what seemed like such a big jump to 10 degrees. Thank you again :) Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From JNocito <@t> Pathreflab.com Thu Jul 15 10:29:10 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT In-Reply-To: <20040715145456.97644.qmail@web52409.mail.yahoo.com> Message-ID: Christine, I'm a private lab that receives legs from a hospital in red biohazard bags, transported by a commercial courier service. I haven't heard of this. I don't think there are any reusable containers that are long enough. I mean, what if the patient has 48 inch legs? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Christine Baker Sent: Thursday, July 15, 2004 9:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LEG TRANSPORT I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Thu Jul 15 10:36:44 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] changing from high to low profile blades In-Reply-To: <61E9F2400F53D5119CFC00508B44E33B019F5612@khmcexch.uhsi.org> References: <61E9F2400F53D5119CFC00508B44E33B019F5612@khmcexch.uhsi.org> Message-ID: <40F6A48C.9000809@bitstream.net> With the Reichert 2030 (and other models) that require that you switch-out the entire back pressure plate when you go from High to Low (or vise versa) blades, it has been my experience that if you do not get the back plate assembled correctly, you will have problems. Try taking the back plate off and re-installing it, making sure the it is properly aligned and the fastening screws are tightened equally. Although it is common to be quick to judge, it's not always the blade that is the problem. Assuming that the back plate is not the problem, you will need to increase the blade angle from what it was with the high profile blades when switching to low profile. This could take a few "trial & errors", so be patient. From working on this "side of the bench" for many years, the split is almost 50/50 as to the number of high to low profile blades that I sell. So they both must work equally as well. But ...like so many things in Histology, we are dealing in an art as much as a science ...so personal preference and personal experience comes into play here. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, llc 1200 Mendelssohn Ave. N., Ste. 50 Minneapolis, MN 55427 (800) 565-1895, ext. 17 Kapoor, Sue wrote: >Hi all, > I have a Reichert-Jung 2030 microtome that I recently changed the back >plate on so that we can use low profile blades. We're expriencing alot of >problems in sectioning now, finding it extremely difficult to get a ribbon. >The sections just compress at the blade. I've tried adjusting the angle, (it >had been at 3 micron), didn't seem to help....any suggestions??????!!!??? > >Thank you, >Sue Kapoor, HT (ASCP) >Histology Coordinator >Kenosha Medical Center >Kenosha, WI >262-653-5570 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From juan.gutierrez <@t> christushealth.org Thu Jul 15 10:31:19 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: Who told you that? That's the way we have always done it here. Please keep me posted. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Christine Baker [mailto:brokeponi@yahoo.com] Sent: Thursday, July 15, 2004 9:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LEG TRANSPORT I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Thu Jul 15 10:45:47 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT In-Reply-To: <20040715145456.97644.qmail@web52409.mail.yahoo.com> References: <20040715145456.97644.qmail@web52409.mail.yahoo.com> Message-ID: <40F6A6AB.4090302@bitstream.net> I'm sure that something more specific to this application has come out by now, but way back when... all limbs were transported to the lab wrapped (completely) in sterile surgical towels place on a stainless steel surgical tray. After examination, any "disposable" parts were then put in the red biohazard bags and disposed of in the proper manner. The surgical towels were also placed in a separate red bag and sent back to Central Service for sterilization and laundering. Then repackaged & autoclaved for re-use. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, llc 1200 Mendelssohn Ave. N. Ste. 50 Minneapolis, MN 55427 (800) 565-1895, Ext. 17 Christine Baker wrote: >I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? > Thanking you in advance >Christine Baker >SRRMC Histology Supervisor > > >--------------------------------- >Do you Yahoo!? >Read only the mail you want - Yahoo! Mail SpamGuard. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From JMahoney <@t> alegent.org Thu Jul 15 10:40:20 2004 From: JMahoney <@t> alegent.org (Janice A Mahoney) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: We use these really great transport bins from MOPEC. They are all OSHA compliant. Jan Mahoney Omaha NE >>> Christine Baker 07/15/2004 9:54:56 AM >>> I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Thu Jul 15 11:11:03 2004 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] employment opportunity in Florida Message-ID: <20040715161103.HCTA13926.mta13.adelphia.net@mail.adelphia.net> We are currently seeking a fulltime technician or technologist in our new dermpath lab in southeast Florida. Florida license (or eligible) and ASCP certification required. We offer newer equipment, a clean and safe work environment, minutes from the ocean for outdoor activities. How many of you get to take an hour lunch at the beach??? We are also in the process of building a new lab. This is a great opportunity for the right person. Ron Martin, BS,HT (ASCP)HTL fax 561-721-1249 From bhewlett <@t> cogeco.ca Thu Jul 15 12:01:44 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Platelets in FFPE References: Message-ID: <004f01c46a8d$69282c70$6500a8c0@mainbox> Jackie, Immunohistochemistry for either CD41 or CD61 (platelet glycoproteins) will do the trick. CD61 certainly works on FFPE and on AZF-fixed and decalcified bone marrow cores, as illustrated in the photo I am sending to you in a separate personal e-mail. Regards, Bryan ----- Original Message ----- From: To: Sent: Thursday, July 15, 2004 11:11 AM Subject: [Histonet] Platelets in FFPE > Does anyone have a tried and true method for demonstrating resting or > activated platelets in FFPE? If not, how about frozen sections? Zinc > fixed tissues? > ANYTHING? > I love you guys - > Jackie O'Connor > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From komo <@t> bu.edu Thu Jul 15 12:09:26 2004 From: komo <@t> bu.edu (Rob Komorowski) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Curled Brain Tissue In-Reply-To: <200407151534.i6FFY3Jx004032@relay7.bu.edu> Message-ID: Not sure if anyone has any hints for this, but I have a few brains of experimental rat brains that were sliced on a cryostat and hat curled, never to flatten again. This has made getting an even stain on then very difficult. Does anyone have any hints as to how to get them to unroll? I'm staining them for c-Fos immuno and they were perfused with paraformaldehyde. Thanks for any help! Rob K From laurie.colbert <@t> huntingtonhospital.com Thu Jul 15 12:14:11 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BFAC@EXCHANGE1.huntingtonhospital.com> We cannot transport any specimen in red biohazard bags per the Department of Health. Red bags designate trash. Surgery now places limbs in a different-colored bag, such as blue or even clear (semi-transparent). We store the limbs in the same bag and when they are ready for disposal (pickup from our outside company), they are then placed in red bags. Laurie Colbert Huntington Hospital -----Original Message----- From: Christine Baker [mailto:brokeponi@yahoo.com] Sent: Thursday, July 15, 2004 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LEG TRANSPORT I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Jul 15 12:16:46 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BFAD@EXCHANGE1.huntingtonhospital.com> By the way, in reference to my last response on legs - I am in California. In response to Joe's question about finding a bin big enough for the legs, we used to place them in the containers that you can buy for storing wrapping paper. -----Original Message----- From: Christine Baker [mailto:brokeponi@yahoo.com] Sent: Thursday, July 15, 2004 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LEG TRANSPORT I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kspencer <@t> utmem.edu Thu Jul 15 12:27:45 2004 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Curled Brain Tissue In-Reply-To: Message-ID: <48BCC076-D684-11D8-9614-000393967904@utmem.edu> Rob, This is exactly the same problem that we have had for a year now. We have tried everything except buying a new cryostat. We also need to do c-fos on floating sections. Another guy e-mailed me yesterday with the same problem. He said his sections looked like little burritos sitting in the bottom of the well. Ours do too. What kind of cryostat do you have? Also, give me as many details as you can, section thickness etc. Kathleen Spencer HT (ASCP) Lab Manager/ LCM Supervisor UTHSC On Thursday, July 15, 2004, at 12:09 PM, Rob Komorowski wrote: > Not sure if anyone has any hints for this, but I have a few brains of > experimental rat brains that were sliced on a cryostat and hat curled, > never > to flatten again. This has made getting an even stain on then very > difficult. Does anyone have any hints as to how to get them to unroll? > I'm > staining them for c-Fos immuno and they were perfused with > paraformaldehyde. > Thanks for any help! > > Rob K > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jluis.palazon <@t> icman.csic.es Thu Jul 15 12:39:07 2004 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] kupffer cell Message-ID: <20040715173907.832A524578E@perceval.uca.es> Dear Histonetters First I would like to say hello to all of you after a time out of the histonett. I would like to stablish the presence of kupffer cells in a fish species. I know that one of the procedures consist of inyecting ink to the animal and then the cells appear black in the slides but I dont know the detailed procedure used. can any of you please let me know the detailed procedure indicating if some changes in tissue embedding is necesary?. It does not matter if the procedure if for a mammalian I will adapt it to fish. thanks in advance Jos? Luis From cforster <@t> umn.edu Thu Jul 15 12:55:15 2004 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] curled sections Message-ID: <40F6C503.4090100@umn.edu> For those trying to cut thick cryosections, it is not uncommon for them to curl. If you are going to run IHC on floating sections just place them into your post -fix or PBS if they are fixed, right when you cut them. They will flatten out. Run your stains per floating protocol. When done developing, float in a staining dish of distilled water and mount onto charged slides, airdry completely, dip into xylene, coverslip, done. It works well for me. Questions, call me. Colleen Forster U of MN Division of Neuropathology 612-626-0436 From minkk <@t> zgi.com Thu Jul 15 13:08:16 2004 From: minkk <@t> zgi.com (KMIN (Kathy Mink)) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry staining mach ines Message-ID: We use both the OPTIMAX PLUS and the Ventana Benchmark and like them both. The 40 slide capacity is the only down side of the Biogenix machine..... Kathy Mink ZymoGenetics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marion Hiles Sent: Thursday, July 15, 2004 7:04 AM To: 'GUTIERREZ, JUAN'; Kamal Dawwas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fully automated immunohistochemistry staining mach ines We use the OPTIMAX PLUS machine manufactured by Biogenex and its brilliant. It is only a 40 slide capacity machine but I think there is a larger capacity one out there. Bob Quilty Neuropathology Dept Frenchay Hospital BRISTOL, UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GUTIERREZ, JUAN Sent: 15 July 2004 14:53 To: Kamal Dawwas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Ventana's Benchmark XT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorised. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sulekhababy <@t> yahoo.co.uk Thu Jul 15 14:07:02 2004 From: sulekhababy <@t> yahoo.co.uk (=?iso-8859-1?q?Sulekha=20Ravi?=) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Re Eosin fading Message-ID: <20040715190702.17244.qmail@web25006.mail.ukl.yahoo.com> Hi We had that problem of eosin fading when we were using water soluble eosin. Then we changed over to spirit soluble eosin ( 1% in alcohol ) and solved that problem permanently. Sulekha --------------------------------- ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself From sulekhababy <@t> yahoo.co.uk Thu Jul 15 14:15:59 2004 From: sulekhababy <@t> yahoo.co.uk (=?iso-8859-1?q?Sulekha=20Ravi?=) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Hematoxylin Fading Message-ID: <20040715191559.13070.qmail@web25005.mail.ukl.yahoo.com> Hello everybody Thank you all for helping me to solve the problem of fading of Hematoxylin. I was using DPX (Merck) as mountant. Help me to get an alternative mountant. Sulekha --------------------------------- ALL-NEW Yahoo! Messenger - sooooo many all-new ways to express yourself From joseph-galbraith <@t> uiowa.edu Thu Jul 15 14:25:52 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] IHF with blue conjugated secondaries Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008606@uihc-mail1.uihc.uiowa.edu> Danielle: You may want to address your question directly to Chris van der Loos who is arguably the world's best expert in multiple staining techniques. You can get his address by querying a previous email posting of his on the histonet. I have his address but I don't know how he feels about other people giving it out. He often responds to questions such as yours on this forum. Good luck. Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Danielle Crippen Sent: Wednesday, July 14, 2004 5:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHF with blue conjugated secondaries Dear All, I have an investigator who is in need of triple fluorescence labeling on mouse brain tissue. The green fluorophore is injected and the request is that we double label for two additional antigens. Our problem is that we cannot find a blue conjugated secondary that works at all. We've purchased AMCA Dn x Rb (Jackson) and 350 Dn x Gt (Molecular Probes) and have tried them at every possible working concentration recommended on the spec sheets. We've included GFAP positive controls with Cy3 secondary (this works beautifully) and we've tried using alternate tissue to eliminate the possibility that it's our particular sample. We've also tried varying the blocking method and several different protocols. Considering the confines of our microscope set-up, we must use a blue and a red conjugated secondary. Additionally, one of our primary antibodies is made in goat, so both our secondaries must be made in donkey. Any suggestions as to vendors, protocols or particular references from the literature are VERY welcome!!! Many many thanks in advance!! Danielle Crippen Morphology Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Thu Jul 15 14:34:34 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] endogenous alk phos block and HIER Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008607@uihc-mail1.uihc.uiowa.edu> Amy: We do our blocking prior to retrieval. We've had similar discussions in the lab in the past and our experiments have failed to demonstrate any large difference, whether blocking is before or after retrieval. We have not detected any return of background that had been previously blocked as a result of the heat retrieval approaches that we routinely use. Obviously some experimentation using your own methods would be best as the intensity and conditions of retrieval techniques do vary. We keep our heat retrieval approaches standardized but also minimized - just enough to do the trick - which means that our approach tends to be less intense than some labs but our staining still strong and clean. Good luck Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy Porter Sent: Thursday, July 15, 2004 9:46 AM To: Histonet Subject: [Histonet] endogenous alk phos block and HIER Just curious to know what everyone thinks about pretreatments for endogenous products and Heat induced retrieval methods. Do you do the blocking before the retrieval or after? I am always worried about effecting the pH treatment from the retrieval if i pretreat for endogenous after retrieval. On the other hand if I block first and then retrieve, am i re-opening what i just tried to get rid of while retrieving. I hope this makes sense to someone and opinions would be greatly appreciated. I know i will get lots of good information, as always - Thanks in advance for all the help. Amy S.Porter, HT(ASCP) Michigan State University Department of Physiology Division of Human Pathology College of Human Medicine portera203@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Mail is new and improved - Check it out! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Jul 15 14:47:17 2004 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] R-J 2030 Universal Cassette Clamp Message-ID: Hi All, I've recently started a new job. At my last job we had two Reichert-Jung 2030s, and I grew very attached to mine. Well, my new lab has one, and no one else likes to use it so I want to claim it for my own, but it doesn't have a universal clamp. They have a little block of wood stuck in the clamp so Unisette cassettes will fit, but then you can't take the block out and put it back in because the angle of the block has changed.....and who has time to fiddle around lining up every block you recut. Anyway, my question is, does anyone have a universal cassette clamp for a R-J 2030 that they wouldn't mind parting with? Vendors feel free to reply. The machine is 15 years old, so I really don't want to put a lot of money into a clamp. I will barter> Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From funderwood <@t> mcohio.org Thu Jul 15 14:57:20 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:44 2005 Subject: [BULK] - [Histonet] R-J 2030 Universal Cassette Clamp Message-ID: This came up once before and I jotted down this info; Leica microsystems tech assistance center 1.800.248.0123 ext. 5918 also, I think that surgipath may carry a compatible universal clamp. good luck, Fred >>> "Angela Bitting" 07/15/04 03:47PM >>> Hi All, I've recently started a new job. At my last job we had two Reichert-Jung 2030s, and I grew very attached to mine. Well, my new lab has one, and no one else likes to use it so I want to claim it for my own, but it doesn't have a universal clamp. They have a little block of wood stuck in the clamp so Unisette cassettes will fit, but then you can't take the block out and put it back in because the angle of the block has changed.....and who has time to fiddle around lining up every block you recut. Anyway, my question is, does anyone have a universal cassette clamp for a R-J 2030 that they wouldn't mind parting with? Vendors feel free to reply. The machine is 15 years old, so I really don't want to put a lot of money into a clamp. I will barter> Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Jul 15 16:14:39 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: <35.49c5a90a.2e284dbf@aol.com> Amputated extremities in red biohazard bags: I've received them that way in dozens of pathology services over the last 20 years, and never heard of any bureaucrat or manager attempting to prohibit it. Funeral directors pick up body parts, and also fetuses, in small suitcase-like bags. You could ask a funeral director what they use and how to get it. I hope I'm allowed to reach into a red biohazard bag - I've used them as an overnight dust cover for microscopes for years! Bob Richmond Samurai Pathologist Knoxville TN and Gastonia NC From swaram <@t> myrealbox.com Thu Jul 15 16:27:42 2004 From: swaram <@t> myrealbox.com (Swaram) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] endogenous alk phos block and HIER In-Reply-To: <5D03ED7B9391D4119D9B0008C76B7B2403008607@uihc-mail1.uihc.uiowa.edu> References: <5D03ED7B9391D4119D9B0008C76B7B2403008607@uihc-mail1.uihc.uiowa.edu> Message-ID: <40F6F6CE.8060101@myrealbox.com> Dear Joe, Interesting..!! Have you found an increase in background fluorescence proportional to the time used for heat based Ag retrieval in old paraffin embedded tissue? Can microwave and pressure cooking can be used for all antigens or does it vary for Ag to Ag? Does any histonetter have any experience with fluorescent IHC of telomerase using microwave techQ? Thanks Swaram Galbraith, Joe wrote: >Amy: > >We do our blocking prior to retrieval. We've had similar discussions in the lab in the past and our experiments have failed to demonstrate any large difference, whether blocking is before or after retrieval. We have not detected any return of background that had been previously blocked as a result of the heat retrieval approaches that we routinely use. Obviously some experimentation using your own methods would be best as the intensity and conditions of retrieval techniques do vary. We keep our heat retrieval approaches standardized but also minimized - just enough to do the trick - which means that our approach tends to be less intense than some labs but our staining still strong and clean. > >Good luck > >Joe Galbraith > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy >Porter >Sent: Thursday, July 15, 2004 9:46 AM >To: Histonet >Subject: [Histonet] endogenous alk phos block and HIER > > >Just curious to know what everyone thinks about pretreatments for endogenous products and Heat induced retrieval methods. Do you do the blocking before the retrieval or after? I am always worried about effecting the pH treatment from the retrieval if i pretreat for endogenous after retrieval. On the other hand if I block first and then retrieve, am i re-opening what i just tried to get rid of while retrieving. I hope this makes sense to someone and opinions would be greatly appreciated. I know i will get lots of good information, as always - Thanks in advance for all the help. > > >Amy S.Porter, HT(ASCP) >Michigan State University >Department of Physiology >Division of Human Pathology >College of Human Medicine >portera203@yahoo.com > > > >--------------------------------- >Do you Yahoo!? >Yahoo! Mail is new and improved - Check it out! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mari.ann.mailhiot <@t> leica-microsystems.com Thu Jul 15 16:42:20 2004 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] R-J 2030 Universal Cassette Clamp Message-ID: Angela Give me a call and I can give you a part number for the universal cassette clamp. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Angela Bitting" To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] R-J 2030 Universal Cassette Clamp western.edu 07/15/2004 02:47 PM Hi All, I've recently started a new job. At my last job we had two Reichert-Jung 2030s, and I grew very attached to mine. Well, my new lab has one, and no one else likes to use it so I want to claim it for my own, but it doesn't have a universal clamp. They have a little block of wood stuck in the clamp so Unisette cassettes will fit, but then you can't take the block out and put it back in because the angle of the block has changed.....and who has time to fiddle around lining up every block you recut. Anyway, my question is, does anyone have a universal cassette clamp for a R-J 2030 that they wouldn't mind parting with? Vendors feel free to reply. The machine is 15 years old, so I really don't want to put a lot of money into a clamp. I will barter> Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Laboratories (570)214-9634 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Pathologyarts <@t> aol.com Thu Jul 15 16:43:25 2004 From: Pathologyarts <@t> aol.com (Pathologyarts@aol.com) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] thick and thin sections Message-ID: <84.2e37d14f.2e28547d@aol.com> i have a friend who says she is getting thick and thin sections because the block can slide laterally in the block holder. using a 2025 my blocks also move but i don't have any trouble with the thick and thin sections. the side to side movement doesn't seem to make sense to me, anyone have any input, ever seen anything like this? From LuckG <@t> empirehealth.org Thu Jul 15 17:12:59 2004 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT Message-ID: Hello Christine, Laurie et. al., We use the "Pathport 2 Tissue Transport System" available from Mopec (www.mopec.com / 800.362.8491) for transport and holding of extremities. We have used them for 10 years and they turned out to be a good solution to our problem. We have 3 of them (catalog # BE017) and change the liner pads (catalog # BE018) with each specimen. They are large (I've only had two specimens that wouldn't fit intact, hemipelvectomies) and they are made of heavy durable plastic which can easily be steam cleaned if desired. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Thursday, July 15, 2004 10:14 AM To: Christine Baker; Histonet (E-mail) Subject: RE: [Histonet] LEG TRANSPORT We cannot transport any specimen in red biohazard bags per the Department of Health. Red bags designate trash. Surgery now places limbs in a different-colored bag, such as blue or even clear (semi-transparent). We store the limbs in the same bag and when they are ready for disposal (pickup from our outside company), they are then placed in red bags. Laurie Colbert Huntington Hospital -----Original Message----- From: Christine Baker [mailto:brokeponi@yahoo.com] Sent: Thursday, July 15, 2004 7:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LEG TRANSPORT I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jul 15 17:36:31 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Curled Brain Tissue In-Reply-To: References: <200407151534.i6FFY3Jx004032@relay7.bu.edu> Message-ID: <6.0.0.22.0.20040715155509.01ad66b0@gemini.msu.montana.edu> We just set up hamster brain perfused with Periodate Lysine Paraformaldehyde, cut into coronal slices after a few hours immersion in PLP. Slices were slices cryoprotected overnight in 25% sucrose in PBS. Snap freezing was done using empty petri dish floating on liquid nitrogen inside styrofoam box. The slice of brain (coronal) was embedded in OCT in Tissue Tek mold and allowed to freeze inside extremely cold petri dish. One piece of tissue per block. Cryosectioning was done with Leica 1850 using that model's disposable knife holder with Accuedge high profile blade and the glass anti-roll device. Knife angle was set at 12 degrees. Temperature at knife, sample and anti-roll plate was -19C, micrometer set at 50 um. Antiroll plate was cleaned with alcohol and dried before sectioning. Block was oriented so it looked like a diamond shape, cutting on a point was path of least resistance and for capture with brush when necessary. The section passed under roll plate smoothly, without compression, totally flat until it hit warmer area of antiroll plate. As section finishes coming off block, it does begin to curl back towards knife edge. This problem was solved by capturing end of curled OCT area with a brush (long, thin, fine bristles), and holding section to keep it from curling while antiroll plate was raised. With careful manipulation, the section was picked up onto slide (lowering slide to section). Another brush was employed to hold top of section if it was needed. At no time did the brush touch tissue, only OCT - making it easy to play with the section. Also tried some "heavy breathing" onto section which helped it stay flat until slide pickup time, just don't melt the section! Another technic was tried - after capturing section to keep it from curling up, the section was slid onto top of a totally cold Plus charge slide (had been stored in cryostat). After section was on slide, I slide was warmed on back of hand - section melted onto glass. This was more difficult with thick 50 um section than with a thin 5 um section. It was easier to reach under anti roll plate to keep section from curling rather than slide section onto cold slide and melt it. These onery thicker sections just loved to curl up! Sectioning was done in a slow, steady motion. Evaluation of situation: The section tends to warm up the further it travels under the antiroll plate, causing it to curl. With patience and if one can capture the curl to prevent a major curl up, then you have a chance. No sticking to the antiroll plate was experienced nor wrinkling, compression. We will be trying the dry ice cooling of anti roll plate to see if this solves some of curling problem. Alan Bright gave this helpful, clever hint some time ago. I also wonder if touching the metal plate of knife holder with dry ice will help, anything to keep the warming trend to a minimum. It was impressive to see this anti roll plate work so well, as I am normally a "brush" person. I tried brush but with thicker sections it take a stronger set of bristles to flatten/prevent curling. Sorry for the long description of what was done, but maybe the fine detail will help - From lpwenk <@t> sbcglobal.net Thu Jul 15 17:31:23 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Cholesterol Stains References: <1df.2539f1d5.2e25c957@aol.com> Message-ID: <007b01c46abb$76191380$3d2dd445@domainnotset.invalid> I don't think it can be done. Cholesterol is a lipid, which dissolves out in the alcohol and xylene of processing. If you have any formalin-fixed tissue left, you could do a frozen section, and then do a lipid stain, or there are a couple of stains that are supposed to be specific for cholesterol involving oxidizing with ferric salts (Schultz method comes to mind). Most of the time, for the FFPE tissues, on the H&E, we look for where the cholesterol USED TO BE. Sort of like white needles in the pale pink plaque areas. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Tuesday, July 13, 2004 7:25 PM Subject: [Histonet] Cholesterol Stains > Hi, > I need to do Cholesterol staining in FFPE tissues. > Any help would be appreciated. > Lin > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jul 15 17:41:15 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] thick and thin sections In-Reply-To: <84.2e37d14f.2e28547d@aol.com> References: <84.2e37d14f.2e28547d@aol.com> Message-ID: <6.0.0.22.0.20040715163759.01b49580@gemini.msu.montana.edu> Look for better cassettes that fit or something is out of adjustment on the clamp. I have noticed some cassettes are different probably due to manufacturing. I have this problem only with megacassettes, and no longer use them except for processing only. At 03:43 PM 7/15/2004, you wrote: >i have a friend who says she is getting thick and thin sections because the >block can slide laterally in the block holder. using a 2025 my blocks also >move >but i don't have any trouble with the thick and thin sections. the side to >side movement doesn't seem to make sense to me, anyone have any input, >ever seen >anything like this? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ccdub <@t> earthlink.net Thu Jul 15 17:48:03 2004 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Leg transport Message-ID: Believe it or not,we have a couple of large Rubbermaid containers. We have put biohazard labels on them and use them to transport legs. We provide each of our hospitals with a container and they use them to send the legs. We have found this to be quite useful. Cindy DuBois, HT ASCP DELTA PATHOLOGY ASSOC. STOCKTON CA From steve8438 <@t> msn.com Thu Jul 15 18:45:09 2004 From: steve8438 <@t> msn.com (LISA MELLO) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT References: Message-ID: Christine, We currently use red biohazard bags for amputations. These are sent to us from the O.R. Currently I have not heard of this regulation that you can not do it this way. Steven Mello,HT(ASCP) Anatomical Pathology Supervisor Cape Cod Hospital Hyannis, MA 02601 ----- Original Message ----- From: Joe Nocito To: Christine Baker ; Histonet@lists.utsouthwestern.edu Sent: Thursday, July 15, 2004 11:29 AM Subject: RE: [Histonet] LEG TRANSPORT Christine, I'm a private lab that receives legs from a hospital in red biohazard bags, transported by a commercial courier service. I haven't heard of this. I don't think there are any reusable containers that are long enough. I mean, what if the patient has 48 inch legs? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Christine Baker Sent: Thursday, July 15, 2004 9:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] LEG TRANSPORT I have just been told that I cannot use the red bags to transport a leg from Surgery to the lab and than to disposal. It is illegal to reach inside the bag to remove anything once it has been put in it. I would like to know how everyone else is handling this problem. Is there a transport vehicle that is just for legs? If so where do i get it? Thanking you in advance Christine Baker SRRMC Histology Supervisor --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 <@t> msn.com Thu Jul 15 18:47:51 2004 From: steve8438 <@t> msn.com (LISA MELLO) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry staining machines References: Message-ID: DAKO Automation. We currently have two units and have been very happy with them. Steven Mello, HT(ASCP) Anatomical Pathology Supervisor Cape Cod Hospital Hyannis, MA 02601 ----- Original Message ----- From: Joe Nocito To: GUTIERREZ, JUAN ; Kamal Dawwas ; histonet@lists.utsouthwestern.edu Sent: Thursday, July 15, 2004 11:25 AM Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Okay, You heard it from me first. Ventana Benchmark XT. I now am the proud owner of 2 XTs. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GUTIERREZ, JUAN Sent: Thursday, July 15, 2004 8:53 AM To: Kamal Dawwas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Ventana's Benchmark XT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From conniegrubaugh <@t> hotmail.com Thu Jul 15 21:13:08 2004 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Histology Position In Las Vegas Nv Message-ID: Available immediately is a full time third shift Histology position. Three years experience with HT or HTL. Fax resume to Karen Hackworth Histology Manager at 702-948-8542 Connie G. _________________________________________________________________ [1]FREE pop-up blocking with the new MSN Toolbar get it now! References 1. http://g.msn.com/8HMAENUS/2734??PS=47575 From rockbeki <@t> ufl.edu Thu Jul 15 21:47:08 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] thick and thin sections Message-ID: <950892948.1089946028250.JavaMail.osg@spnode33> I've occassionally gotten slightly assymetrical cuts due to the block moving in the microtome. I just usually take that to mean that I need push the block farther in and look at it from the side to make sure its straight before cut again. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From rockbeki <@t> ufl.edu Thu Jul 15 21:53:46 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] endogenous alk phos block and HIER Message-ID: <993819247.1089946426084.JavaMail.osg@spnode33> I'm using peroxidase but I do my blocking for endogenous peroxidase after the AR, and that seems to be working. I have accidentally reverse it before though, and didn't get wildly different results, so I'm not sure whether the order matters much. Rebekah Smith On Thu Jul 15 10:46:27 EDT 2004, Amy Porter wrote: > Just curious to know what everyone thinks about pretreatments for > endogenous products and Heat induced retrieval methods. Do you > do the blocking before the retrieval or after? I am always > worried about effecting the pH treatment from the retrieval if i > pretreat for endogenous after retrieval. On the other hand if I > block first and then retrieve, am i re-opening what i just tried > to get rid of while retrieving. I hope this makes sense to > someone and opinions would be greatly appreciated. I know i will > get lots of good information, as always - Thanks in advance for > all the help. > > > Amy S.Porter, HT(ASCP) Michigan State University Department of > Physiology Division of Human Pathology College of Human Medicine > portera203@yahoo.com > > > > --------------------------------- > Do you Yahoo!? > Yahoo! Mail is new and improved - Check it out! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From abright <@t> brightinstruments.com Fri Jul 16 06:25:42 2004 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Curled Brain Tissue Message-ID: I have stood back on this issue as the last time I tried to be of assistance on this subject I was flamed, however I would not like to deprive others of some useful information that could be of use to those of you with difficulties in brain sectioning. They are tips that I have posted some time ago on Histonet and I would be please to email this information to you. Please reply offline directly to me. Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 15 July 2004 23:37 To: Rob Komorowski; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Curled Brain Tissue We just set up hamster brain perfused with Periodate Lysine Paraformaldehyde, cut into coronal slices after a few hours immersion in PLP. Slices were slices cryoprotected overnight in 25% sucrose in PBS. Snap freezing was done using empty petri dish floating on liquid nitrogen inside styrofoam box. The slice of brain (coronal) was embedded in OCT in Tissue Tek mold and allowed to freeze inside extremely cold petri dish. One piece of tissue per block. Cryosectioning was done with Leica 1850 using that model's disposable knife holder with Accuedge high profile blade and the glass anti-roll device. Knife angle was set at 12 degrees. Temperature at knife, sample and anti-roll plate was -19C, micrometer set at 50 um. Antiroll plate was cleaned with alcohol and dried before sectioning. Block was oriented so it looked like a diamond shape, cutting on a point was path of least resistance and for capture with brush when necessary. The section passed under roll plate smoothly, without compression, totally flat until it hit warmer area of antiroll plate. As section finishes coming off block, it does begin to curl back towards knife edge. This problem was solved by capturing end of curled OCT area with a brush (long, thin, fine bristles), and holding section to keep it from curling while antiroll plate was raised. With careful manipulation, the section was picked up onto slide (lowering slide to section). Another brush was employed to hold top of section if it was needed. At no time did the brush touch tissue, only OCT - making it easy to play with the section. Also tried some "heavy breathing" onto section which helped it stay flat until slide pickup time, just don't melt the section! Another technic was tried - after capturing section to keep it from curling up, the section was slid onto top of a totally cold Plus charge slide (had been stored in cryostat). After section was on slide, I slide was warmed on back of hand - section melted onto glass. This was more difficult with thick 50 um section than with a thin 5 um section. It was easier to reach under anti roll plate to keep section from curling rather than slide section onto cold slide and melt it. These onery thicker sections just loved to curl up! Sectioning was done in a slow, steady motion. Evaluation of situation: The section tends to warm up the further it travels under the antiroll plate, causing it to curl. With patience and if one can capture the curl to prevent a major curl up, then you have a chance. No sticking to the antiroll plate was experienced nor wrinkling, compression. We will be trying the dry ice cooling of anti roll plate to see if this solves some of curling problem. Alan Bright gave this helpful, clever hint some time ago. I also wonder if touching the metal plate of knife holder with dry ice will help, anything to keep the warming trend to a minimum. It was impressive to see this anti roll plate work so well, as I am normally a "brush" person. I tried brush but with thicker sections it take a stronger set of bristles to flatten/prevent curling. Sorry for the long description of what was done, but maybe the fine detail will help - _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Fri Jul 16 06:53:06 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Fully automated immunohistochemistry stainingmachines Message-ID: To all, Again I must say, I like Dako stainers best of all. They are open or bar coded systems. You can choose any combination of chemistries. Service is great. Jo Mauger >>> "LISA MELLO" 07/15/04 07:47PM >>> DAKO Automation. We currently have two units and have been very happy with them. Steven Mello, HT(ASCP) Anatomical Pathology Supervisor Cape Cod Hospital Hyannis, MA 02601 ----- Original Message ----- From: Joe Nocito To: GUTIERREZ, JUAN ; Kamal Dawwas ; histonet@lists.utsouthwestern.edu Sent: Thursday, July 15, 2004 11:25 AM Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Okay, You heard it from me first. Ventana Benchmark XT. I now am the proud owner of 2 XTs. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GUTIERREZ, JUAN Sent: Thursday, July 15, 2004 8:53 AM To: Kamal Dawwas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fully automated immunohistochemistry staining machines Ventana's Benchmark XT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _________________________________________________________________ Want to block unwanted pop-ups? Download the free MSN Toolbar now! http://toolbar.msn.co.uk/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Fri Jul 16 07:29:11 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] Kidney Biopsies Message-ID: How is anyone freezing their kidney biopsies. Can we dab some eosin or something on them to keep them visable? Annette Featherstone Supervisor, Kaleida Health Anatomic Path -----Original Message----- From: SMITH,REBEKAH FELICIA [mailto:rockbeki@ufl.edu] Sent: Thursday, July 15, 2004 22:47 To: Pathologyarts@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] thick and thin sections I've occassionally gotten slightly assymetrical cuts due to the block moving in the microtome. I just usually take that to mean that I need push the block farther in and look at it from the side to make sure its straight before cut again. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From tpschaer <@t> vet.upenn.edu Fri Jul 16 07:33:37 2004 From: tpschaer <@t> vet.upenn.edu (Tom Schaer) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT References: Message-ID: <007201c46b31$1e1a34e0$38595b82@vet.upenn.edu> Greetings: This sounds to me like one of these EHRS rules....why don't you cut the bag open and peel the leg out without reaching into the bag proper. This way you don not reach into the bag if this is the only violation. The EHRS people have their rules and we have our interpretations to their rules. In my recent experience, if it were for EHRS, nothing ever would happen because it is too risky and whatever... Cheers, tom ------------------------------------ Thomas P. Sch?r, VMD Asst Prof School of Biomedical Engineering, Drexel University & Comparative Orthopaedic Research & Tissue Engineering Department of Clinical Studies University of Pennsylvania New Bolton Center 382 West Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office) tel. 610-444-5800 (x6131 lab) fax. 610-925-8100 tpschaer@mail.vet.upenn.edu http://www2.vet.upenn.edu/labs/corl/drtps.html ----- Original Message ----- From: "LISA MELLO" To: "Christine Baker" ; ; "Joe Nocito" Sent: Thursday, July 15, 2004 7:45 PM Subject: Re: [Histonet] LEG TRANSPORT > Christine, > We currently use red biohazard bags for amputations. These are sent to us from the O.R. Currently I have not heard of this regulation that you can not do it this way. > Steven Mello,HT(ASCP) > Anatomical Pathology Supervisor > Cape Cod Hospital > Hyannis, MA 02601 > ----- Original Message ----- > From: Joe Nocito > To: Christine Baker ; Histonet@lists.utsouthwestern.edu > Sent: Thursday, July 15, 2004 11:29 AM > Subject: RE: [Histonet] LEG TRANSPORT > > > Christine, > I'm a private lab that receives legs from a hospital in red biohazard bags, > transported by a commercial courier service. I haven't heard of this. I > don't think there are any reusable containers that are long enough. I mean, > what if the patient has 48 inch legs? > > Joe Nocito, BS, HT(ASCP) QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Christine > Baker > Sent: Thursday, July 15, 2004 9:55 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] LEG TRANSPORT > > I have just been told that I cannot use the red bags to transport a leg from > Surgery to the lab and than to disposal. It is illegal to reach inside the > bag to remove anything once it has been put in it. I would like to know how > everyone else is handling this problem. Is there a transport vehicle that is > just for legs? If so where do i get it? > Thanking you in advance > Christine Baker > SRRMC Histology Supervisor > > > --------------------------------- > Do you Yahoo!? > Read only the mail you want - Yahoo! Mail SpamGuard. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sue.Kapoor <@t> uhsi.org Fri Jul 16 07:54:44 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] high to low profile blades Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5615@khmcexch.uhsi.org> We were using high profile blades from Surgipath and the microtome was set at 3 degrees. Changing the back plate to switch to low profile blades, we just couldn't get sections. I kept thinking it had to be the angle but I didn't think it would be such a big jump. I have it set at 10 degrees now and so far so good. Thanks again for all the suggestions, working it out on my own would have taken awhile to get as high as 10. just curious....anyone else using a Reichert-Jung 2030 with high profile blades, what is your angle set at? Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Thursday, July 15, 2004 2:09 PM To: Kapoor, Sue Subject: Re: [Histonet] high to low profile blades Interesting, I set all my high profiles on a Leica at 11 degrees, tweaking low profile a tidge to the high side of 12 or 13. It must be the blades you were using, I can honestly say I have never used a disposable blade (low or high) at 3 degrees! From bill501 <@t> mindspring.com Fri Jul 16 08:15:56 2004 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] LEG TRANSPORT In-Reply-To: <007201c46b31$1e1a34e0$38595b82@vet.upenn.edu> References: <007201c46b31$1e1a34e0$38595b82@vet.upenn.edu> Message-ID: At 8:33 AM -0400 7/16/04, Tom Schaer wrote: >EHRS What is EHRS? -- ______________ Bill Blank, MD Heartland Lab From haldana <@t> unimoron.edu.ar Fri Jul 16 08:26:08 2004 From: haldana <@t> unimoron.edu.ar (Hernan Aldana) Date: Fri Sep 16 15:23:44 2005 Subject: [Histonet] RE: curl sections In-Reply-To: <200407152347250.SM01484@swlx162.swmed.edu> Message-ID: <001001c46b38$74d42fa0$6fac59c8@b3w6zzmtb6juvbs> For those trying to cut thick cryosections, it is not uncommon for them to curl. Just place them into your post -fix floating sections or PBS if they are fixed, right when you cut them. They will flatten out. Mount onto charged or gelatin coated slides. Air-dry for a minutes ant then run your Stains or immuno previous bath in distilled water. Per floating protocol lose a lot of regents.. When done dip into alcohols, xylene, coverslip. From funderwood <@t> mcohio.org Fri Jul 16 08:55:09 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:45 2005 Subject: [BULK] - [Histonet] thick and thin sections Message-ID: Maybe her clamp is gooped up with paraffin waste and the clamp does not spring shut tightly. It may also be the brand of cassettes she is using. Some of them have a little more give, which can be minimized by making sure the cassette is filled to the brim with paraffin at embedding. Fred >>> 07/15/04 05:43PM >>> i have a friend who says she is getting thick and thin sections because the block can slide laterally in the block holder. using a 2025 my blocks also move but i don't have any trouble with the thick and thin sections. the side to side movement doesn't seem to make sense to me, anyone have any input, ever seen anything like this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> polysciences.com Fri Jul 16 09:23:32 2004 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:23:45 2005 Subject: [BULK] - [Histonet] thick and thin sections In-Reply-To: Message-ID: <001f01c46b40$793657a0$4000a8c0@PMARCUM2K> One other issue is overfilling the cassettes during embedding and having an excess of rounded paraffin sticking up above the rim. As this is more rounded it will allow the block to slip and not fit well in the chuck. Look at the back and be sure the paraffin is level across the cassette back as well as full only to the rim. Thanks, Pam Marcum? > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fred > Underwood > Sent: Friday, July 16, 2004 9:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [BULK] - [Histonet] thick and thin sections > > > Maybe her clamp is gooped up with paraffin waste and the clamp does not > spring shut tightly. It may also be the brand of cassettes she is > using. Some of them have a little more give, which can be minimized by > making sure the cassette is filled to the brim with paraffin at > embedding. > > Fred > > >>> 07/15/04 05:43PM >>> > i have a friend who says she is getting thick and thin sections because > the > block can slide laterally in the block holder. using a 2025 my blocks > also move > but i don't have any trouble with the thick and thin sections. the side > to > side movement doesn't seem to make sense to me, anyone have any input, > ever seen > anything like this? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Pat.Willis <@t> mail.bhrv.nwest.nhs.uk Fri Jul 16 08:53:04 2004 From: Pat.Willis <@t> mail.bhrv.nwest.nhs.uk (Pat Willis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] unsubscribe[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D1BD7F9@BHRV_NT_11> From gcallis <@t> montana.edu Fri Jul 16 09:49:51 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Curled Brain Tissue In-Reply-To: References: Message-ID: <6.0.0.22.0.20040716083923.01ac4748@gemini.msu.montana.edu> Dear Alan and all, I'm so sorry you were "flamed" as your suggestions reside in my frozen section file for future use. I have even passed a copy of your Histonet message (privately) onto people experiencing difficulties with brain. I always read your comments on cryomicrotomy with "fine tuning" hints. In fact, I plan to try them IF I encounter any further problems with brain frozen sections. Your expertise on the subject is greatly needed and appreciated by my laboratory. The only flame I would light is in celebration of success for getting good ,thick frozen sections - consider it in honor of your astute, educated suggestions!! Have a good weekend, and keep the suggestions coming - At 05:25 AM 7/16/2004, you wrote: >I have stood back on this issue as the last time I tried to be of >assistance on this subject I was flamed, however I would not like to >deprive others of some useful information that could be of use to those >of you with difficulties in brain sectioning. They are tips that I have >posted some time ago on Histonet and I would be please to email this >information to you. Please reply offline directly to me. > > >Alan Bright > >Bright Instrument Co.Ltd. >St Margaret's Way >Huntingdon >Cambridgeshire >PE29 6EU >England > >Tel No:+44 (0)1480 454528 >Fax No:+44 (0)1480 456031 >Email: abright@brightinstruments.com >Web Site: www.brightinstruments.com > > > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: 15 July 2004 23:37 >To: Rob Komorowski; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Curled Brain Tissue > > >We just set up hamster brain perfused with Periodate Lysine >Paraformaldehyde, cut into coronal slices after a few hours immersion in > >PLP. Slices were slices cryoprotected overnight in 25% sucrose in >PBS. Snap freezing was done using empty petri dish floating on liquid >nitrogen inside styrofoam box. The slice of brain (coronal) was >embedded >in OCT in Tissue Tek mold and allowed to freeze inside extremely cold >petri >dish. One piece of tissue per block. > >Cryosectioning was done with Leica 1850 using that model's disposable >knife >holder with Accuedge high profile blade and the glass anti-roll >device. Knife angle was set at 12 degrees. Temperature at knife, >sample >and anti-roll plate was -19C, micrometer set at 50 um. Antiroll plate >was >cleaned with alcohol and dried before sectioning. Block was oriented so >it >looked like a diamond shape, cutting on a point was path of least >resistance and for capture with brush when necessary. > >The section passed under roll plate smoothly, without compression, >totally >flat until it hit warmer area of antiroll plate. As section finishes >coming off block, it does begin to curl back towards knife edge. This >problem was solved by capturing end of curled OCT area with a brush >(long, >thin, fine bristles), and holding section to keep it from curling >while antiroll plate was raised. With careful manipulation, the >section >was picked up onto slide (lowering slide to section). Another brush was > >employed to hold top of section if it was needed. At no time did the >brush >touch tissue, only OCT - making it easy to play with the section. Also >tried some "heavy breathing" onto section which helped it stay flat >until >slide pickup time, just don't melt the section! > >Another technic was tried - after capturing section to keep it from >curling >up, the section was slid onto top of a totally cold Plus charge slide >(had >been stored in cryostat). After section was on slide, I slide was >warmed >on back of hand - section melted onto glass. This was more difficult >with >thick 50 um section than with a thin 5 um section. > >It was easier to reach under anti roll plate to keep section from >curling >rather than slide section onto cold slide and melt it. These onery >thicker >sections just loved to curl up! > >Sectioning was done in a slow, steady motion. > >Evaluation of situation: The section tends to warm up the further it >travels under the antiroll plate, causing it to curl. With patience and >if >one can capture the curl to prevent a major curl up, then you have a >chance. No sticking to the antiroll plate was experienced nor >wrinkling, >compression. > >We will be trying the dry ice cooling of anti roll plate to see if this >solves some of curling problem. Alan Bright gave this helpful, clever >hint >some time ago. I also wonder if touching the metal plate of knife holder > >with dry ice will help, anything to keep the warming trend to a minimum. > >It was impressive to see this anti roll plate work so well, as I am >normally a "brush" person. I tried brush but with thicker sections it >take >a stronger set of bristles to flatten/prevent curling. > >Sorry for the long description of what was done, but maybe the fine >detail >will help - > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From juan.gutierrez <@t> christushealth.org Fri Jul 16 09:58:57 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Kidney Biopsies Message-ID: If you are doing frozen sections for IF you do not want to use eosin, it lights up big time under fluorescent lighting. We use to do our lung sections with and without Evan's Blue, that might help. I think the blue was to get rid of background, so I think you could use it to mark your tissue. Any other ideas out there on the net? Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org] Sent: Friday, July 16, 2004 7:29 AM To: 'SMITH,REBEKAH FELICIA'; Pathologyarts@aol.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Biopsies How is anyone freezing their kidney biopsies. Can we dab some eosin or something on them to keep them visable? Annette Featherstone Supervisor, Kaleida Health Anatomic Path -----Original Message----- From: SMITH,REBEKAH FELICIA [mailto:rockbeki@ufl.edu] Sent: Thursday, July 15, 2004 22:47 To: Pathologyarts@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] thick and thin sections I've occassionally gotten slightly assymetrical cuts due to the block moving in the microtome. I just usually take that to mean that I need push the block farther in and look at it from the side to make sure its straight before cut again. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ivoschi <@t> web.de Fri Jul 16 10:03:10 2004 From: ivoschi <@t> web.de (Ivo Schmidt) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] OCT und freezing time Message-ID: <151626064@web.de> Hello experts, I want to freezer my tissue quickly in OCT to preserve tissue architecture and macromolecules (RNA, DNA, etc) in isopentane at -80?C. Does anybody know, how much longer you should immerse an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ? Thanx in advance. Cordially, Ivo. _______________________________________________________ WEB.DE Video-Mail - Sagen Sie mehr mit bewegten Bildern Informationen unter: http://freemail.web.de/?mc=021199 From kspencer <@t> utmem.edu Fri Jul 16 10:15:32 2004 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] RE: curl sections In-Reply-To: <001001c46b38$74d42fa0$6fac59c8@b3w6zzmtb6juvbs> Message-ID: My original post some time ago was about sections that curl in the buffer, whether they curl on the knife or not. There was a post yesterday, his curled in the buffer only. They curl up so tight that IHC is impossible. You can not say "they will flatten out" any longer, because in many cases, they do not. They should, but they do not. For over one year we have been fighting this problem. And I assure you that we have tried everything. My frustration and despair is monumental. Kathleen On Friday, July 16, 2004, at 08:26 AM, Hernan Aldana wrote: > For those trying to cut thick cryosections, it is not uncommon for them > to curl. Just place them into your post -fix floating sections or PBS if > they are fixed, right when you cut them. They will flatten out. Mount > onto charged or gelatin coated slides. Air-dry for a minutes ant then > run your Stains or immuno previous bath in distilled water. Per floating > protocol lose a lot of regents.. When done dip into alcohols, xylene, > coverslip. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gloria.Niehans <@t> med.va.gov Fri Jul 16 10:35:03 2004 From: Gloria.Niehans <@t> med.va.gov (Niehans, Gloria) Date: Fri Sep 16 15:23:45 2005 Subject: FW: [Histonet] OCT und freezing time Message-ID: <2C7B953912C2D511B6470000F8033DA50146D47F@VHAMINEXC1> -----Original Message----- From: Niehans, Gloria Sent: Friday, July 16, 2004 10:21 AM To: 'Ivo Schmidt' Subject: RE: [Histonet] OCT und freezing time It only adds about a minute to the time when the tissue freezes solid. We usually left OCT-embedded tissue in the isopentane freezing bath at -80 degrees C for between 5 and 10 minutes, although sometimes when we got busy the tissue stayed in the freezing bath for up to an hour and it didn't seem to hurt the tissue at all (isopentane is very inert). We got good recovery of RNA and DNA out of tissues handled this way, and tissue architecture was very good (comparable to frozen sections done in the OR). Gloria Niehans -----Original Message----- From: Ivo Schmidt [mailto:ivoschi@web.de] Sent: Friday, July 16, 2004 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT und freezing time Hello experts, I want to freezer my tissue quickly in OCT to preserve tissue architecture and macromolecules (RNA, DNA, etc) in isopentane at -80?C. Does anybody know, how much longer you should immerse an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ? Thanx in advance. Cordially, Ivo. _______________________________________________________ WEB.DE Video-Mail - Sagen Sie mehr mit bewegten Bildern Informationen unter: http://freemail.web.de/?mc=021199 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gloria.Niehans <@t> med.va.gov Fri Jul 16 10:46:43 2004 From: Gloria.Niehans <@t> med.va.gov (Gloria.Niehans@med.va.gov) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Histology Position Open Minneapolis VA Medical Center Message-ID: <2C7B953912C2D511B6470000F8033DA50146D480@VHAMINEXC1> Histonetters: The Minneapolis VA Medical Center has an immediate opening for a full time Histopathology Technician. Responsibilities in Surgical Pathology section of Anatomic Pathology Service. Salary commensurate with education and experience (experienced histotechs are classified in the GS-7 series, current pay $34,220 to $44,485 depending on extent of experience; recent grads start at a lower grade). Sign-on bonus. Position offers full federal benefits package, including: vacation--minimum 13 days, sick leave, free parking, retirement plan and 401K, health and life insurance. For further details about the position you can call Pat Rene, Anatomic Pathology Supervisor, at (612) 725-2000 ext. 2467, or you can E-mail me at Gloria.Niehans@med.va.gov. From cwscouten <@t> myneurolab.com Fri Jul 16 11:34:43 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] OCT und freezing time Message-ID: If you use the OCT sparingly, it should not add any significant time. The tissue freezes in seconds. One or two more seconds, OK. Note that you can store the tissue in the isopentane for months no problem. It will need to temperature equilibrate in the cyrostat, but keep that period as short as possible to avoid ice crystal reformation. We offer an instrument for isopentane freezing, so you always have the cold fluid availbable, and it doesn't evaporate. See the link below: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=476401&catdesc=Histology+Equipment&CatThreeID=650&CatOneID=4&subcatdesc=Freezing+Devices&idsubcategory=187 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ivo Schmidt Sent: Friday, July 16, 2004 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT und freezing time Hello experts, I want to freezer my tissue quickly in OCT to preserve tissue architecture and macromolecules (RNA, DNA, etc) in isopentane at -80?C. Does anybody know, how much longer you should immerse an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ? Thanx in advance. Cordially, Ivo. _______________________________________________________ WEB.DE Video-Mail - Sagen Sie mehr mit bewegten Bildern Informationen unter: http://freemail.web.de/?mc=021199 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Jul 16 11:25:26 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] OCT und freezing time In-Reply-To: <151626064@web.de> References: <151626064@web.de> Message-ID: <6.0.0.22.0.20040716102129.01acdc68@gemini.msu.montana.edu> At this temperature, I assume dry ice is cooling the isopentane aka 2 methyl butane at ~-80C - snap freezing time is basically the same. At 09:03 AM 7/16/2004, you wrote: >Hello experts, > >I want to freezer my tissue quickly in OCT to preserve >tissue architecture and macromolecules (RNA, DNA, etc) >in isopentane at -80?C. >Does anybody know, how much longer you should immerse >an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ? > >Thanx in advance. > >Cordially, > >Ivo. > > >_______________________________________________________ >WEB.DE Video-Mail - Sagen Sie mehr mit bewegten Bildern >Informationen unter: http://freemail.web.de/?mc=021199 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From csawrenk <@t> bccancer.bc.ca Fri Jul 16 12:20:48 2004 From: csawrenk <@t> bccancer.bc.ca (Sawrenko, Christina) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Galectin-3 Message-ID: <6BAF4D075F07D411B30900508B94CBA09C6D55@SERVER20> Good morning, One of our pathologists has asked us to research Galectin-3 for use in thyroid neoplasms. Does anyone have experience with this antibody and do you use it in conjuction with CD44v6 (Novocastra)? Thanks in advance for your help! Chris Sawrenko Histopathology BC Cancer Agency Vancouver BC Canada From conniegrubaugh <@t> hotmail.com Thu Jul 15 13:25:57 2004 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Histology Position in Las Vegas Message-ID: Laboratory Medicine Consultants has a full time position HT ASCP with 3 years experience for the third shift. Please fax all resume's to Karen Hackworth Histology Manager 702-938-3620 Connie G. _________________________________________________________________ [1]Is your PC infected? Get a FREE online computer virus scan from McAfee® Security. References 1. http://g.msn.com/8HMBENUS/2755??PS=47575 From conniegrubaugh <@t> hotmail.com Thu Jul 15 20:17:01 2004 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Histology Position In Las Vegas Nv Message-ID: Available immediately is a full time third shift Histology position. Three years experience with HT or HTL. Fax resume to Karen Hackworth Histology Manager at 702-948-8542 Connie G. _________________________________________________________________ [1]MSN Life Events gives you the tips and tools to handle the turning points in your life. References 1. http://g.msn.com/8HMBENUS/2743??PS=47575 From conniegrubaugh <@t> hotmail.com Wed Jul 14 11:49:00 2004 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Histology Position Open in Las Vegas Nevada Message-ID: Laboratory Medicine Consultants has a full time Histology third shift position available. Must be HT or HTL certified. Prefer 3 years experience. Fax resume to Karen Hackworth. 702-938-3620 Connie G. _________________________________________________________________ [1]Is your PC infected? Get a FREE online computer virus scan from McAfee® Security. References 1. http://g.msn.com/8HMBENUS/2755??PS=47575 From rgrow <@t> bmnet.com Fri Jul 16 13:24:09 2004 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Re: Fully automated immunohistochemistry staining machines Message-ID: DakoCytomation. We can do 48 slides at a time. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax -----Original Message----- From: Kamal Dawwas [mailto:kdawwas@hotmail.com] Sent: Thursday, July 15, 2004 1:54 AM To: histonet@lists.utsouthwestern.edu< mailto:histonet@lists.utsouthwestern.edu> Subject: [Histonet] Fully automated immunohistochemistry staining machines I am interested in the views of the people who have used any of these machines, and which in their openion is the best in the market. kjdawwas King Khalid University Hospital Saudia Arabia _______________________________________________________________ This E-mail contains PRIVILEGED AND CONFIDENTIAL INFORMATION intended only for the use of the Individual(s) named above. If you are not the intended recipient of this E-mail, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this E-mail is strictly prohibited. If you have received this E-mail in error, please immediately notify us at 865-977-5652 or notify us by E-mail at helpdesk@bmnet.com From akbitting <@t> geisinger.edu Fri Jul 16 13:50:40 2004 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Costing for TMA Message-ID: Has anyone calculated the tech time it takes to cut slides from TMA blocks? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From carl.hobbs <@t> kcl.ac.uk Fri Jul 16 14:19:32 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] re Alan Bright and being flamed Message-ID: <000a01c46b69$d33d6210$c9949a51@home> I don't know the details of that but I greatly respect Bright Instruments for their excellent equipment and superb backup. They have always, for me, been genuinely concerned with any technical problems relating to their equipment and their resolution. I have never seen a post by Alan that is blatant advertising; they have been constructive and relevant to the query raised. I always read them and I hope Alan continues to input into the site. Carl Hobbs Laboratory Manager Wolfson Centre for Age-Related Diseases Wolfson Wing Hodgkin building Guys Campus Kings College London SE11UL --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 From Rcartun <@t> harthosp.org Fri Jul 16 15:14:48 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Kidney Biopsies Message-ID: I freeze all my kidney needle core specimens (usually 18 gauge) in O.C.T. in a cryostat. I don't think that you need to color them. Richard Cartun >>> "Featherstone, Annette" 07/16/04 08:29AM >>> How is anyone freezing their kidney biopsies. Can we dab some eosin or something on them to keep them visable? Annette Featherstone Supervisor, Kaleida Health Anatomic Path -----Original Message----- From: SMITH,REBEKAH FELICIA [mailto:rockbeki@ufl.edu] Sent: Thursday, July 15, 2004 22:47 To: Pathologyarts@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] thick and thin sections I've occassionally gotten slightly assymetrical cuts due to the block moving in the microtome. I just usually take that to mean that I need push the block farther in and look at it from the side to make sure its straight before cut again. -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Virginia.Achstetter <@t> afip.osd.mil Fri Jul 16 08:06:52 2004 From: Virginia.Achstetter <@t> afip.osd.mil (Achstetter, Virginia A.) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] (no subject) Message-ID: <9C631520464F9E4BA5B111450F2A0AAD0BCA18@lewis.afip.osd.mil> We are about to purchase a tissue microarrayer from Beecher Instruments. I see that Chemicon also puts one out. Has anyone out there ever compared the two? Ginny Achstetter HT (ASCP) Armed Forces Institute of Pathology Soft Tissue Pathology 6825 16th St. NW Bldg 54 Rm. 3118 Washington, DC 20306 202-782-2813 From histo20 <@t> hotmail.com Fri Jul 16 14:35:20 2004 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] QC Issues Message-ID: Hi everyone, After speaking with several of my area colleagues, a couple of questionable QC isssues came up. Does anyone run an HE control on their frozen section set-up? Is the use of Cyto-cool banned from frozens and in the cutting of permanent blocks? Does everyone decontaminate the cryostat with bleach at least once a month? Does everyone have control block documentation? And do your pathologists do a daily/weekly quality control check sheet? Thanks so much for your input! Paula Wilder St. Joseph Medical Center Towson, MD _________________________________________________________________ Discover the best of the best at MSN Luxury Living. http://lexus.msn.com/ From slimwillie <@t> cox.net Fri Jul 16 23:48:01 2004 From: slimwillie <@t> cox.net (Jerry Wilson) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] (Histonet) Sakura Auto Stainer Message-ID: <009d01c46bb9$3d40df80$f2340e44@no.cox.net> I am currently considering purchasing a Sakura Automatic slide stainer- DRS 6000 (i think). I would like to set this instrument up to stain both Histology and Cytology slides. I understand the unit has only 27 resevoirs for reagents, but can any of the resevoirs be commonly used by both procedures? Or, to get to the point- does anyone do this with their stainer and would they be willing to share the procedure? Thanks Jerry Wilson From slimwillie <@t> cox.net Fri Jul 16 23:16:10 2004 From: slimwillie <@t> cox.net (Jerry Wilson) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Sakura Autostainer Message-ID: <004a01c46bb4$ca7d2160$f2340e44@no.cox.net> I am currently considering purchasing a Sakura Automatic slide stainer- DRS 6000 (i think). I would like to set this instrument up to stain both Histology and Cytology slides. I understand the unit has only 27 resevoirs for reagents, but can any of the resevoirs be commonly used by both procedures? Or, to get to the point- does anyone do this with their stainer and would they be willing to share the procedure? Thanks Jerry Wilson From hmcleod <@t> chempath.uct.ac.za Sat Jul 17 03:46:45 2004 From: hmcleod <@t> chempath.uct.ac.za (Mcleod) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] CEA Message-ID: <40F8E775.C63E064B@chempath.uct.ac.za> Dear All A colleague has asked me to post a question on her behalf. She needs to find a vendor for CEA Clone B-80. The usual suppliers do not seem to have it (i.e Dako., Novo., BD and Labvision) Thankyou Heather From sandeeptem <@t> rediffmail.com Sat Jul 17 06:51:20 2004 From: sandeeptem <@t> rediffmail.com (Sandeep Gopalakrishnan) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] best fixative for EM_IHC Message-ID: <20040717115120.8213.qmail@mailweb33.rediffmail.com> hi histonetters, Could any one give a best for electron microscopy studies. I karnovsky's fixative. Is that a best one? Simila (cacodylate or phhospahte buffer ) is the best to prepare karnovsky's fixative. Since cacodylate is stable i am presently using thanks in advance. Sandeep Gopalakrishnan Transmission Electron Microscopy Labora Division of Implant Biology Biomedical Technolo Satelmond palace campus Sree Chitra&nb Poojapura, Tr Kerala state,India Phone: Cell :9846387770 [1] [ad=] References 1. 3D"http://clients.rediff.=/ From sandeeptem <@t> rediffmail.com Sat Jul 17 06:51:45 2004 From: sandeeptem <@t> rediffmail.com (Sandeep Gopalakrishnan) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] best fixative for EM_IHC Message-ID: <20040717115145.8608.qmail@mailweb33.rediffmail.com> hi histonetters, Could any one give a best for electron microscopy studies. I karnovsky's fixative. Is that a best one? Simila (cacodylate or phhospahte buffer ) is the best to prepare karnovsky's fixative. Since cacodylate is stable i am presently using thanks in advance. Sandeep Gopalakrishnan Transmission Electron Microscopy Labora Division of Implant Biology Biomedical Technolo Satelmond palace campus Sree Chitra&nb Poojapura, Tr Kerala state,India Phone: Cell :9846387770 [1] [ad=] References 1. 3D"http://clients.rediff.=/ From sandeeptem <@t> rediffmail.com Sat Jul 17 06:52:08 2004 From: sandeeptem <@t> rediffmail.com (Sandeep Gopalakrishnan) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] best fixative for EM_IHC Message-ID: <20040717115208.23492.qmail@mailweb34.rediffmail.com> hi histonetters, Could any one give a best for electron microscopy studies. I karnovsky's fixative. Is that a best one? Simila (cacodylate or phhospahte buffer ) is the best to prepare karnovsky's fixative. Since cacodylate is stable i am presently using thanks in advance. Sandeep Gopalakrishnan Transmission Electron Microscopy Labora Division of Implant Biology Biomedical Technolo Satelmond palace campus Sree Chitra&nb Poojapura, Tr Kerala state,India Phone: Cell :9846387770 [1] [ad=] References 1. 3D"http://clients.rediff.=/ From jnocito <@t> satx.rr.com Sat Jul 17 07:28:02 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] QC Issues References: Message-ID: <013b01c46bf9$81103bd0$676ace44@yourxhtr8hvc4p> Paula, we don't perform frozen sections, so we don't have a cryostat and frozen staining setup. We do have a control block log for immunos and special stains. We have it set up this way DATE SURG # STAIN PATH DATE TESTED When the pathologists gives a positive case we just write it in the book. Every case that has a special or immuno has a sheet to go with it. It has the DATE SURG # STAIN TECH DR REMARKS COMMENTS My medical director signs off on all temp charts, maintenance schedules QC sheets at the end of the month. If youo want, please email me your fax number and I'll fax you some examples. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Paula Wilder" To: Sent: Friday, July 16, 2004 2:35 PM Subject: [Histonet] QC Issues > Hi everyone, > After speaking with several of my area colleagues, a couple of questionable > QC isssues came up. Does anyone run an HE control on their frozen section > set-up? Is the use of Cyto-cool banned from frozens and in the cutting of > permanent blocks? Does everyone decontaminate the cryostat with bleach at > least once a month? Does everyone have control block documentation? And do > your pathologists do a daily/weekly quality control check sheet? > Thanks so much for your input! > Paula Wilder > St. Joseph Medical Center > Towson, MD > > _________________________________________________________________ > Discover the best of the best at MSN Luxury Living. http://lexus.msn.com/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jryan <@t> sleh.com Sat Jul 17 12:08:27 2004 From: jryan <@t> sleh.com (John Ryan) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] I'm sorry I can not reply immediately because I am out of the office, returning on Monday July 26, Message-ID: I'm sorry I can not reply immediately because I am out of the office, returning on Monday July 26, 2004. From tahseen <@t> brain.net.pk Sat Jul 17 14:10:47 2004 From: tahseen <@t> brain.net.pk (Muhammad Tahseen) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Fully automated immunohistochemistry staining machines References: Message-ID: <00b501c46c31$c5137300$972bfea9@m7c0y4> Dear Kamal DakoCytomation. We currently have one unit and have been very happy with them. We can do 48 slides at a time. Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore PAKISTAN. ----- Original Message ----- From: Kamal Dawwas To: Sent: Thursday, July 15, 2004 11:54 AM Subject: [Histonet] Fully automated immunohistochemistry staining machines > > I am interested in the views of the people who have used any of these > machines, and which in their openion is the best in the market. > > > kjdawwas > King Khalid University Hospital > Saudia Arabia > > _________________________________________________________________ > Want to block unwanted pop-ups? Download the free MSN Toolbar now! > http://toolbar.msn.co.uk/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tissuearray <@t> hotmail.com Sat Jul 17 15:55:39 2004 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Costing for TMA Message-ID: I don't know if anyone has tracked the time and cost for cutting TMA blocks. I think the price would depend on the quality of the array blocks. Some are constructed well and some are not. I have seen and cut many different types and found it hard to justify an exact time. But I am a very skilled array technician and would consider independently contracting for cutting array blocks. My website below is helpful for those who would like to learn more about array construction techniques. Thom Jensen For more information on Tissue Microarray instruction visit: arrayworkshop.com >From: "Angela Bitting" >To: >Subject: [Histonet] Costing for TMA >Date: Fri, 16 Jul 2004 14:50:40 -0400 > >Has anyone calculated the tech time it takes to cut slides from TMA blocks? > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Express yourself instantly with MSN Messenger! Download today - it's FREE! References 1. http://g.msn.com/8HMAENUS/2752??PS=47575 From histojock <@t> hotmail.com Sat Jul 17 16:42:11 2004 From: histojock <@t> hotmail.com (Histo Jock) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Re: Costong for TMA Message-ID: There's probably too many factors involved to make a decent estimate. Are you using tape transfer or a water bath? What size punches are you using? How experienced is the tech? etc. etc. etc. I've seen techs take a perfect section in a few seconds, others that take minutes to get a decent cut. It's all dependant on your individual setup. HistoJock. >------------------------------ > >From: "Angela Bitting" >Subject: [Histonet] Costing for TMA > >Has anyone calculated the tech time it takes to cut slides from TMA blocks? _________________________________________________________________ Planning a family vacation? Check out the MSN Family Travel guide! http://dollar.msn.com From pruegg <@t> ihctech.net Sat Jul 17 16:52:39 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Fixative and H2O2 for frozen In-Reply-To: Message-ID: I do use freshly prepared fixative and h202 each time. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jorge Ivan Zapata Valencia Sent: Saturday, May 15, 2004 12:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixative and H2O2 for frozen Hello everybody a technical question: may I use the same acetone:etanol mix to fix samples different days, or do I have to prepare it feshly every day?? The same with the H2O2 PBS solution for endogenous peroxidase blocking. Is it mandatory to use fresh one? Thank You very much jorge ivan zapata _________________________________________________________________ The new MSN 8: advanced junk mail protection and 2 months FREE* http://join.msn.com/?page=features/junkmail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 17 16:53:03 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] re caspase 3 and pcna In-Reply-To: <004401c44505$e8957b00$3c292bd9@home> Message-ID: Yep, I am now using Ki67 instead of PCNA. Paty -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Carl Sent: Friday, May 28, 2004 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re caspase 3 and pcna I understand ( tho I may be corrected and informed further), that PCNA is not the best marker to use, if you are assessing cells in cycle. It was, before Ki67 and H3? --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.690 / Virus Database: 451 - Release Date: 22/05/2004 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 17 16:53:06 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] methyl green In-Reply-To: Message-ID: Inga, Did it used to stain spinal cord satifactorily? If it did and now it doesn't I would say make it fresh and see if it gets better. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Inga Hansson Sent: Monday, May 31, 2004 5:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] methyl green Hi histonetters! Can anyone explain to me why my methyl green counterstaining stopped working?? I use 0.5% solution and have had it for a while. Should it be made fresh?? Spinal cord seems to be the least stained! Cerebellum the best. Is there a difference between different cell types?? Thanks in advance! Inga _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 17 16:52:42 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] DAB (DAKO and Vector) In-Reply-To: <74D0F0AB07F2E647A02D839ED79520F9B48745@VAIEXCH02.vai.org> Message-ID: I have used Envision with many DAB's including vector, it works fine. My favorite continues to be the Bragatti Stable DAB, but don't buy it from Invitrogen, get it from Phoenix Biologicals it is better quality. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan Sent: Friday, May 14, 2004 12:41 PM To: histonet@pathology.swmed.edu Subject: [Histonet] DAB (DAKO and Vector) Hi, Thanks all who replied to my query on pressure cookers. I have a question for those who have used the Envision + kit. Has anyone used it with the Vector DAB kit, rather than the DAB+ from DAKO? Thanks! Min-han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 17 16:52:27 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Fixation and Decalcification of Bone Marrow Bxs. In-Reply-To: <4FE843CBBAC1D211A8150008C70952DAAE7E01@hk01nt05.hkh.wgkk.sozvers.at> Message-ID: Alex, Try using zinc formalin in place of your NBF with the EDTA, it should improve your H&E morphology. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nader, Alexander Sent: Sunday, May 16, 2004 11:53 PM To: Histology Net List Server (E-Mail) Subject: RE: [Histonet] Fixation and Decalcification of Bone Marrow Bxs. > About 6 months ago, we switched to fixing our bone marrows > in 10% NBF saturated with EDTA (this fixes and decals at the > same time) > with beautiful results with our IHC and also ISH. Even though our > pathologists are elated that the IHC and ISH are great, they are not > completely happy with the morphology of the H&E slides. I was just > wondering if anyone has any suggestions so we can accomplish > both great H&E morphology and great stains with IHC and ISH. What is your formula for the NBF-EDTA mixture? pH? Alexander Nader MD Vienna, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 17 16:53:10 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Favorite DAB? In-Reply-To: <001001c44995$0a0cc3e0$e3f2acd1@powellsa1> Message-ID: Dito to Shirley, I use the stable DAB from Phoenix, it is the old Research Genetics (Bragatti) DAB, don't buy it from Invitrogen it is not the same and not as good in my hands. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shirley Powell Sent: Thursday, June 03, 2004 11:03 AM To: Johnson, Teri; histonet@pathology.swmed.edu Subject: Re: [Histonet] Favorite DAB? I use Stable DAB from Phoenix Bio Technologies (formerly Research Genetics I think) in Huntsville, AL 1-866-319-0900. Works very well and is reasonably priced. Shirley ----- Original Message ----- From: "Johnson, Teri" To: Sent: Thursday, June 03, 2004 11:47 AM Subject: [Histonet] Favorite DAB? Hi all, I'm looking for alternatives for my current DAB vendor (this stuff's expensive!) and wanted to poll you all to see what your favorite DAB is. I'm looking for stability, ease of use, good sensitivity and nice color reaction. Thanks for your help! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jul 17 16:49:06 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] anti-alk phos Message-ID: is anyone aware of an antibody to alk phos to label osteoblasts in decalcified ffpe bone samples? please provide info on where to get it if you know it is out there. Thanks, Patsy From zhangl <@t> utas.edu.au Sun Jul 18 02:10:46 2004 From: zhangl <@t> utas.edu.au (zhangl) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] protocol for freezing fixed rat skeletal muscle In-Reply-To: <200407171707.i6HH7Huo024583@tweedledee.its.utas.edu.au> Message-ID: <5.1.0.14.0.20040718160503.00aebdb0@postoffice.sandybay.utas.edu.au> Hi Histontters, Thanks for the response to my question "mapping capillary perfusion pattern". Here I have another question need your help. I am going to perfuse rat hindlimb with BSA+FITC-dextran, then to locate the FITC-dextran in frozen muscle tissue. To prevent FITC-dextran from diffusing out of tissue when I mount the tissue onto a slide, will some fixation before freezing the tissue be helpful? Can anyone kindly share a good protocol for freezing fixed muscle tissue? fixative? how long the fixation? any cryoprotection? By the way, I wonder if anyone knows whether the FITC will bleach during the fixation, freezing process? Thanks in advance. Lei Zhang Biochemistry, medicine school University of Tasmania, Australia 61 03 6226 2669 zhangl@utas.edu.au From tahseen <@t> brain.net.pk Sun Jul 18 06:49:28 2004 From: tahseen <@t> brain.net.pk (Muhammad Tahseen) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Her2 Neu by PCR Message-ID: <00f001c46cbd$48fd6000$972bfea9@m7c0y4> Dear All A colleague has asked me to post some question on his behalf. He is a research fellow he needs to find informations about Detection of Her2 Neu by PCR method. Details of primers Details of Tag Details of probe If there is complete detection set available, company name & address. Thankyou Muhammad Tahseen Histology Supervisor SKMCH&RC Lahore PAKISTAN. From rockbeki <@t> ufl.edu Sun Jul 18 10:37:22 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Fixative and H2O2 for frozen Message-ID: <2056071312.1090165042055.JavaMail.osg@spnode33> I second that. My prof always told me its important to make up the peroxide new every day. Rebekah Smith On Sat Jul 17 17:52:39 EDT 2004, Patsy Ruegg wrote: > I do use freshly prepared fixative and h202 each time. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Jorge > Ivan Zapata Valencia > Sent: Saturday, May 15, 2004 12:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fixative and H2O2 for frozen > > > Hello everybody > a technical question: may I use the same acetone:etanol mix to > fix samples > different days, or do I have to prepare it feshly every day?? The > same with > the H2O2 PBS solution for endogenous peroxidase blocking. Is it > mandatory to > use fresh one? > Thank You very much > > jorge ivan zapata > > _________________________________________________________________ > The new MSN 8: advanced junk mail protection and 2 months FREE* > http://join.msn.com/?page=features/junkmail > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From JColCLEFA <@t> aol.com Sun Jul 18 15:44:24 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] ihc stainers, methyl green, ki67, DAB Message-ID: <3EDB9872.6155BE74.0229601F@aol.com> i have a high volume lab and although the software is clunky, we have a biogenex optimax (40 sl) and 3 biogenex i6000's (60 slides each). we run them all simultaneously daily, (max 220 slides) sometimes up to 3x daily and we do the majority of troubleshooting and some servicing ourselves when necessary. I recommend them highly, but would like to try the newer Dako items of the volume could be matched. I make up methyl green fresh each use b/c I notice the staining reaction dies shortly in storage. i've been using KI67 forever and still trust it. DAB- When I want brown /black, I use biogenex concentrate kits. When I want golden brown, I use Dako Kits. (both are relatively stable after mixing, The difference in color is important for choosing multiple stain reagents, not so much for single applications. we make up H2O2 weekly, store at 4C and have no issue with background due to endogenous peroxidase. From lpwenk <@t> sbcglobal.net Sun Jul 18 16:25:41 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Kidney Biopsies References: Message-ID: <009301c46d0d$c77d5a20$b036d445@domainnotset.invalid> Instead of trying to stain the tissue a different color (so the white-ish tissue can be seen against the white freezing media), why not buy a freezing media that is a different color than the tissue, such as one that is blue? Several companies sell the colored freezing media, which can be used to either allow the tissue to be seen or to keeping track of tissues easier - e.g. 6 different patients come in for FS all at the same time - patient 1 FS is pink, pt. 2 FS is blue, pt. 3 is yellow, etc - e.g. keeping track of different margins from same tissue - 12 to 3 pm is pink, 3 to 6 pm is blue, etc.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Featherstone, Annette" To: "'SMITH,REBEKAH FELICIA'" ; ; Sent: Friday, July 16, 2004 8:29 AM Subject: [Histonet] Kidney Biopsies > How is anyone freezing their kidney biopsies. Can we dab some eosin or > something on them to keep them visable? > Annette Featherstone > Supervisor, Kaleida Health Anatomic Path > > -----Original Message----- > From: SMITH,REBEKAH FELICIA [mailto:rockbeki@ufl.edu] > Sent: Thursday, July 15, 2004 22:47 > To: Pathologyarts@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] thick and thin sections > > > I've occassionally gotten slightly assymetrical cuts due to the > block moving in the microtome. I just usually take that to mean > that I need push the block farther in and look at it from the side > to make sure its straight before cut again. > > -- > SMITH,REBEKAH FELICIA > "You are a child of the universe, no less than the trees and the > stars > You have a right to be here and whether or not it is clear to you, > no doubt the universe is unfolding as it should. Therefore be at > peace with G-d, whatever you conceive Him to be. And whatever your > labors and aspirations,in the noisy confusion of life, keep peace > in your soul.-Max Ehrmann,"Desiderata" > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: > This email transmission and any documents, files, > or previous e-mail messages attached to it are > confidential and intended solely for the use of the > individual or entity to whom they are addressed. > If you are not the intended recipient, or a person > responsible for delivering it to the intended recipient, > you are hereby notified that any further review, > disclosure, copying, dissemination, distribution, or > use of any of the information contained in or attached > to this e-mail transmission is strictly prohibited. > If you have received this message in error, please > notify the sender immediately by e-mail, discard > any paper copies, and delete all electronic files > of the message. If you are unable to contact the > sender or you are not sure as to whether you > are the intended recipient, please e-mail > ISTSEC@KaleidaHealth.org or call (716) 859-7777. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Mon Jul 19 03:24:44 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] re Alan Bright and being flamed Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111383@mius2.medlan.cam.ac.uk> I concur with Carl's message and would add that Alan is a very competent cryotomist and any advice he gives should be taken seriously. I'm sorry he got "flamed". Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Carl [mailto:carl.hobbs@kcl.ac.uk] Sent: Friday, July 16, 2004 8:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re Alan Bright and being flamed I don't know the details of that but I greatly respect Bright Instruments for their excellent equipment and superb backup. They have always, for me, been genuinely concerned with any technical problems relating to their equipment and their resolution. I have never seen a post by Alan that is blatant advertising; they have been constructive and relevant to the query raised. I always read them and I hope Alan continues to input into the site. Carl Hobbs Laboratory Manager Wolfson Centre for Age-Related Diseases Wolfson Wing Hodgkin building Guys Campus Kings College London SE11UL --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stephen.Eyres <@t> sanofi-synthelabo.com Mon Jul 19 04:27:16 2004 From: Stephen.Eyres <@t> sanofi-synthelabo.com (Stephen.Eyres@sanofi-synthelabo.com) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] re Alan Bright and being flamed Message-ID: Just to add further to Carl and Margaret's comments, Bright Instruments is, as far as I am aware, the only UK source of cryotomy training. Although geared to their own instruments, the excellent training is relevant to all budding cryotomists. Cheers Steve Margaret Blount To: 'Carl' Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@lists.utsouth cc: western.edu Subject: RE: [Histonet] re Alan Bright and being flamed 19/07/2004 09:24 I concur with Carl's message and would add that Alan is a very competent cryotomist and any advice he gives should be taken seriously. I'm sorry he got "flamed". Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Carl [mailto:carl.hobbs@kcl.ac.uk] Sent: Friday, July 16, 2004 8:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re Alan Bright and being flamed I don't know the details of that but I greatly respect Bright Instruments for their excellent equipment and superb backup. They have always, for me, been genuinely concerned with any technical problems relating to their equipment and their resolution. I have never seen a post by Alan that is blatant advertising; they have been constructive and relevant to the query raised. I always read them and I hope Alan continues to input into the site. Carl Hobbs Laboratory Manager Wolfson Centre for Age-Related Diseases Wolfson Wing Hodgkin building Guys Campus Kings College London SE11UL --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------- Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu?il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d?en aviser imm?diatement l?exp?diteur par retour de courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuelles pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ---------------------------------------------------------- From jluis.palazon <@t> icman.csic.es Mon Jul 19 06:28:55 2004 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] kupffer cell Message-ID: <20040719112855.EA0E124E99F@perceval.uca.es> Dear Histonetters First I would like to say hello to all of you after a time out of the histonett. I would like to stablish the presence of kupffer cells in a fish species. I am not on the sanitary-medical field so I am not used with some histological or lab procedures used in sanitary work. I know that one of the procedures consist of inyecting ink to the animal and then the cells appear black in the slides but I dont know the detailed procedure used (i.e. quantity, pure ink?, where to inject, how long must wait until sacrify the animal, etc). Can any of you please let me know the detailed procedure indicating if some changes in tissue embedding is necesary?. It does not matter if the procedure if for a mammalian I will adapt it to fish. thanks in advance Jos? Luis From mcc12 <@t> cornell.edu Mon Jul 19 07:17:20 2004 From: mcc12 <@t> cornell.edu (Marlene Nardi) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Bouin's alternative Message-ID: <5.2.1.1.2.20040719080624.009ee890@postoffice7.mail.cornell.edu> We currently use Bouin's solution to fix animal eyes (good retinal preservation), to fix animal reproductive tissues (prevents shrinkage of testes), and as the post-fix in the Masson Trichrome stain. Any ideas on a less hazardous alternative solution for any of these uses? For the Trichrome stain I have tried the commercial Bouin's substitute, Gram's iodine and citrate buffer with limited/no success. thank you in advance, Marlene ===== Marlene C. Nardi Technical Services Supervisor Histology Laboratory New York State College of Veterinary Medicine Cornell University Department of Biomedical Sciences S2-124 Schurman Hall Ithaca, NY 14853 mailto:mcc12@cornell.edu ph. 607-253-3314 or 607-253-3303 FAX 607-253-3357 From Stephen.Eyres <@t> sanofi-synthelabo.com Mon Jul 19 08:05:03 2004 From: Stephen.Eyres <@t> sanofi-synthelabo.com (Stephen.Eyres@sanofi-synthelabo.com) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Bouin's alternative Message-ID: Hi Marlene, We use Davidson's fluid, which is a mixture of Alcohol, formalin and acetic acid. Steve Marlene Nardi To: histonet@lists.utsouthwestern.edu Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] Bouin's alternative western.edu 19/07/2004 13:17 We currently use Bouin's solution to fix animal eyes (good retinal preservation), to fix animal reproductive tissues (prevents shrinkage of testes), and as the post-fix in the Masson Trichrome stain. Any ideas on a less hazardous alternative solution for any of these uses? For the Trichrome stain I have tried the commercial Bouin's substitute, Gram's iodine and citrate buffer with limited/no success. thank you in advance, Marlene ===== Marlene C. Nardi Technical Services Supervisor Histology Laboratory New York State College of Veterinary Medicine Cornell University Department of Biomedical Sciences S2-124 Schurman Hall Ithaca, NY 14853 mailto:mcc12@cornell.edu ph. 607-253-3314 or 607-253-3303 FAX 607-253-3357 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------- Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu?il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d?en aviser imm?diatement l?exp?diteur par retour de courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuelles pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ---------------------------------------------------------- From pam <@t> ategra.com Mon Jul 19 09:48:34 2004 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Job Opportunities in Histology Latest Update 07/16/04 Message-ID: Hi Histonetters, Here is the latest update on opportunities with some of my best clients throughout the US who are seeking Histology Supervisors, Histo Technologists and Histo Technicians. These are positions as direct employees of our client. There are fulltime 40 hour per week positions.. As a direct employee of one of our clients you will be provided with full benefits including Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative sign-on bonus. I have part time and temporary positions as well. I have supervisory, team lead and bench positions. These positions require HTL or HT certification or registry eligibility. Here are some of my HOTTEST Histology Supervisor positions: 1. Alabama - Histology Supervisor 2. Colorado - Histology Manager/PA 3. Virginia - Histology Supervisor Here are some of my HOTTEST Histo Tech and Histology Supervisor positions: 1. Georgia - Histo Tech 2. Pennsylvania - Histo Tech 3. Arizona - Histo Tech 4. Texas - Histo Tech temp to perm 5. Maine - Histo Tech 6. Maine - IHC Tech 7. N. California - Histo Tech 8. North Carolina - Histo Tech 9. NYC - Lead Histo Tech 10. NYC - IHC Tech 11. Virginia - Histo Tech 12. Michigan - Histo Tech 13. Nebraska - Histo Tech 14. Nevada - Histo Tech (3rd shift) If you are interested in these jobs, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well. I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. However, if you are interested in any of the jobs above, please call me. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: Pam Barker Senior Lab Recruiter Ategra Systems Inc Specialists in Permanent & Contract Staffing 7085 University Blvd. Winter Park, FL 32792 VOICE: 407-671-5800 ext 234 TOLLFREE: 800-466-9919 ext 234 EMAIL: pam@ategra.com To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. -------------------------------------------------------------------------------------------------- From kspencer <@t> utmem.edu Mon Jul 19 09:50:40 2004 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] cryostats Message-ID: <0103E8B8-D993-11D8-AFFB-000393967904@utmem.edu> Hi all, Question: If you had to buy a new cryostat, and you had to chose between a Hacker Bright or a Leica, which would you purchase, and please tell me why. Mainly I would love to hear from anyone that has had these in the last 10 years. I really need your input on this, detailed input, especially in regards to when you bought the machine that you do not like and how long before it started giving you trouble. Thanks in advance, Kathleen Spencer HT (ASCP) Lab Manager/ LCM Supervisor UTHSC From hborgeri <@t> wfubmc.edu Mon Jul 19 07:38:35 2004 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] anti-alk phos Message-ID: <9AEEF1FB6254224AA355ED285F8491650999C81B@EXCHVS2.medctr.ad.wfubmc.edu> Hi Patsy, I'm not 100% sure, but I think Biomeda and R&D Systems sell an anti-Alkaline Phosphatase antibody. Hermina Hermina M. Borgerink, BA, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax (336) 716-1515 e-mail hborgeri@wfubmc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Saturday, July 17, 2004 5:49 PM To: histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: [Histonet] anti-alk phos is anyone aware of an antibody to alk phos to label osteoblasts in decalcified ffpe bone samples? please provide info on where to get it if you know it is out there. Thanks, Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Mon Jul 19 09:08:21 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Gold Chloride for Retic Message-ID: I have heard that if the Gold Chloride is old it can cause a more "reddish" stain in the retic. Some people are saying it lasts "forever". What is the general consenus? Annette Featherstone HT/MLT -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Saturday, July 17, 2004 17:53 To: Tan, MinHan; histonet@pathology.swmed.edu Subject: RE: [Histonet] DAB (DAKO and Vector) I have used Envision with many DAB's including vector, it works fine. My favorite continues to be the Bragatti Stable DAB, but don't buy it from Invitrogen, get it from Phoenix Biologicals it is better quality. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan Sent: Friday, May 14, 2004 12:41 PM To: histonet@pathology.swmed.edu Subject: [Histonet] DAB (DAKO and Vector) Hi, Thanks all who replied to my query on pressure cookers. I have a question for those who have used the Envision + kit. Has anyone used it with the Vector DAB kit, rather than the DAB+ from DAKO? Thanks! Min-han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Suelutsch <@t> cs.com Mon Jul 19 10:16:47 2004 From: Suelutsch <@t> cs.com (Suelutsch@cs.com) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Urines for DNA staining/Feulgen Message-ID: <9b.4a848ae3.2e2d3fdf@cs.com> I hope someone has the answer for me. We are trying to do Feulgen stain on urines. Have used both a "Thin Prep" and a "Cytospin" and if anything stays on we are lucky. We have tried the Artisan stainer and a manual method. Does anyone have any ideas at all? From histo <@t> bthosp.com Mon Jul 19 10:17:59 2004 From: histo <@t> bthosp.com (O'Brien, Sue) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] cryostats Message-ID: <3653750027C5D6119D0600508BB89A9A0AC095@MAIL_SERVER> Kathleen, I did choose a Leica, and am very satisfied with it. At the time (96) it was the only one with what I felt were great safety features (e.g. vacuum assist for removing tissue shavings) combined with ease of use. I can only say we've had no problems, and I have been very happy with the unit (it's lived up to its promotional material). Regards, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 > -----Original Message----- > From: Kathleen Spencer [SMTP:kspencer@utmem.edu] > Sent: Monday, July 19, 2004 10:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryostats > > Hi all, > > Question: If you had to buy a new cryostat, and you had to chose between > a Hacker Bright or a Leica, which would you purchase, and please tell me > why. Mainly I would love to hear from anyone that has had these in the > last 10 years. > I really need your input on this, detailed input, especially in regards > to when you bought the machine that you do not like and how long before > it started giving you trouble. > > Thanks in advance, > > Kathleen Spencer HT (ASCP) > Lab Manager/ LCM Supervisor > UTHSC > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Mon Jul 19 10:31:33 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] cryostats Message-ID: I have never used a H/B, but I would give it a try. Try both before you buy. I have a Leica 1850 and it had to be replaced within two years. The current one is doing fine. If you get a good one the Leica's are okay except for the blade holder. It has to be taken apart and adjusted every time you change the blade( the pressure clip is to soft). Now if you could choose another company I would try Microm(Richard Allan). Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Kathleen Spencer [mailto:kspencer@utmem.edu] Sent: Monday, July 19, 2004 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostats Hi all, Question: If you had to buy a new cryostat, and you had to chose between a Hacker Bright or a Leica, which would you purchase, and please tell me why. Mainly I would love to hear from anyone that has had these in the last 10 years. I really need your input on this, detailed input, especially in regards to when you bought the machine that you do not like and how long before it started giving you trouble. Thanks in advance, Kathleen Spencer HT (ASCP) Lab Manager/ LCM Supervisor UTHSC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Mon Jul 19 12:47:26 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Rebecca Barnhart/ stainer Message-ID: <000c01c46db8$75253800$1d2a14ac@wchsys.org> Hi, I use a Sakura DRS2000. It has a continuous or one at a time staining run. The DRS2000 stains two racks at a time (40slides). You are only limited by the number of basket adapters you have. (they are not cheap, I have 5 and that seems to handle what we do each day) ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From JNocito <@t> Pathreflab.com Mon Jul 19 13:16:35 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Update on Automated IHC machines Message-ID: Dear fellow 'netters, It appears that my response to which IHC machinery to get has met with some resistance, shock and awe. As a matter of fact, some people have been really angry at me and have directly said so. I know many vendors scan the Histonet and I really don't have a problem with that. Those of us who are in a supervisory or management position know that there are many battles we face every day. We have to learn to pick our battles in order to survive. My battle with which immunostainer to purchase was taken out of my hands by the same people who sign my paychecks. I had my input, but my superiors decided to go in another direction. I had a choice to leave my current position or make things work. Since I have two kids in college, a mortgage and 2 car payments, I decided to make things work. So unless you are willing to provide me with a weekly paycheck, understand that this decision was taken out of my hands. If you can't accept that, then it was a pleasure doing business with you, but I have bills to pay. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From Ben.Shelkowsky <@t> chomp.org Mon Jul 19 13:28:34 2004 From: Ben.Shelkowsky <@t> chomp.org (Shelkowsky, Ben) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Rebecca Barnhart/ stainer Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D6131@exchsrvr.chomp.org> We also use the DRS2000. We also had the DRS (previous model), an older version. We have been told by several cytotechs and inspectors that it's good laboratory practice to not stain gyn or non-gyn (body fluids) slides in the same containers as histo slides. There is the potential to transfer cytology material unto the histo slides and vice versa. So- we then purchased at considerable expense carriers that housed 6 stations and changed the carriers as we did the various stains. This was for the older version. We had 3 carriers for each of the 3 staining runs. The 2 paps (gyn, non-gyn) each had a carrier, and the H&E had a carrier. So throughout the day we kept changing the carriers according to the run needing to be stained, (alot of hassle). Since our numbers went up we now have two SAKURA's, and stain the non-gyn manually. Hope this helps. Ben Shelkowsky CHOMP Monterey, CA 93950 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joyce Cline Sent: Mon 7/19/2004 10:47 AM To: Histonet Cc: Subject: [Histonet] Rebecca Barnhart/ stainer Hi, I use a Sakura DRS2000. It has a continuous or one at a time staining run. The DRS2000 stains two racks at a time (40slides). You are only limited by the number of basket adapters you have. (they are not cheap, I have 5 and that seems to handle what we do each day) ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: 201832d891fa504721b2e650a5a52212 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu Content-Type: text/plain;charset="US-ASCII" Content-Transfer-Encoding: 7bit Subject: [Histonet] Rebecca Barnhart/ stainer X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu X-Spam-Level: X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63 X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) X-eShield-AntiVirus: Passed X-eShield-AntiVirus-Message: Scanned by http://www.bluecoat.com/eShield Return-Path: histonet-bounces@lists.utsouthwestern.edu X-OriginalArrivalTime: 19 Jul 2004 17:55:30.0994 (UTC) FILETIME=[95084120:01C46DB9] Hi, I use a Sakura DRS2000. It has a continuous or one at a time staining run. The DRS2000 stains two racks at a time (40slides). You are only limited by the number of basket adapters you have. (they are not cheap, I have 5 and that seems to handle what we do each day) ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From maria <@t> ski.org Mon Jul 19 13:49:53 2004 From: maria <@t> ski.org (Maria Mejia) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] in-vivo imaging techniques Message-ID: <40FC17D1.4000408@ski.org> Hello Everyone, I posted this message last week (and) I have yet to receive any responses. So, I've decided to send the message...again. In hopes of something. This time my message is a bit more detailed. Keep in mind that the company who make the dye (they have the patent), isn't more helpful. Please, can anyone help, especially the neuroscience folks? One of the PI's I work with is interested in in-vivo imaging techniques. We've been trying for sometime (w/out consist success) to use a particular blue voltage sensitive dye called RH-1692. This type of imaging is a hot new area!! The dye compound is used for low biological noise probing for high-speed in-vivo neural imaging of electrical signals. The problem is in the dye preparation. You wouldn't think this would be a problem, but it is!! I have to weigh very tiny amounts at a time (1mg), the compound is very sticky (and) it's also hygroscopic - sucks up water from the air, thus gets more sticky!! Compound is also light sensitive, so I wrap the vial(s) in foil and work in very dim room. When not is use the dye is stored @ 4 degrees and when used is mixed with either home made or commercial artificial cerebrospinal fluid (CSF) and heated to about 50 F degrees to dissolve. Did I mention that its not easy to dissolve; we've used heating the mixed solution, sonicating it to help it dissolve faster and also have tried using less heat...nothing! The good (but frustrating) news is that it did work twice (2) experiments of of six (6). Which leads us to the possibility that the stock dye might be suspect. We've also used dye with different lot numbers, same results...inconsistancy. So, unless we get some specific details on this dye , hopefully from neuro folks who are generous in sharing information technique and who I can contact directly, we are faced with the possibility, but an expensive fact that we might have to order a new dye vial for each experiment! Sorry for being lengthy. Any information, suggestions, tips...anything anyone can provide, I would greatly appreciate it and look forward to hearing from you. most sincerely, Maria Mejia neurohistologist Smith-Kettlewell Eye Research Insititute San Francisco, CA Email: maria@ski.org Phone: (415)-345-2185/2165 From kwittle <@t> jhmi.edu Mon Jul 19 14:10:05 2004 From: kwittle <@t> jhmi.edu (Karen Wittler) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Dezenkerize Message-ID: Sorry It took me so long to respond. We dissolve iodine in xylene. We have few B5 specimens so we use a coplin jar and treat a couple of slides at a time for five minutes and then hang them on our automated stainer at the first deparaffinizing xylene. As far as the amount of iodine in xylene, It's like a palm full of parsley, season to taste, whatever works. Karen Wittler, HT(ASCP) Johns Hopkins Hospital Baltimore, MD From ihc <@t> unipathllc.com Mon Jul 19 14:57:12 2004 From: ihc <@t> unipathllc.com (UniPath IHC) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Congo Red Message-ID: <000001c46dca$95752400$4500a8c0@unipath02> Has anyone ever heard of doing an overnight Congo Red (overnight in the Congo Red soln.)? One of our Paths is requesting this and we're worried about false positive staining. From Carolyn.Earley <@t> leica-microsystems.com Mon Jul 19 15:00:17 2004 From: Carolyn.Earley <@t> leica-microsystems.com (Carolyn.Earley@leica-microsystems.com) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Sakura Autostainer Message-ID: Hi Jerry, In response to your need to stain Histology and Cytology slides simultaneously, I would just like to let you know that there is another choice of stainers to be Publiclly released by Leica next month. The instrument is designed precisely for your need. There are 5 wash stations with an optional 6th one that can be used with Dist. water if needed. Additionally, there can be up to 32 reagent vessels( which allows enough stations so that sharing is not necessary), 4 ovens, 4 load drawers, and 4 exit drawers depending on how it best serves your needs. The instrument uses an innovative color-coded Transponder Technology to start and complete your multiple protocols simultaneously and if you wish, can be integrated with a coverslipper. Please feel free to call me at the number listed below if you have questions. Good luck in your search. Carolyn Earley Marketing Manager Leica Microsystems 847-405-7014 Carolyn.Earley@Leica-Microsystems.com "Jerry Wilson" To: "histonet@pathology.swmed.edu" Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] Sakura Autostainer western.edu 07/16/2004 11:16 PM I am currently considering purchasing a Sakura Automatic slide stainer- DRS 6000 (i think). I would like to set this instrument up to stain both Histology and Cytology slides. I understand the unit has only 27 resevoirs for reagents, but can any of the resevoirs be commonly used by both procedures? Or, to get to the point- does anyone do this with their stainer and would they be willing to share the procedure? Thanks Jerry Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From bhewlett <@t> cogeco.ca Mon Jul 19 15:12:41 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Congo Red References: <000001c46dca$95752400$4500a8c0@unipath02> Message-ID: <000f01c46dcc$c04c0520$6500a8c0@mainbox> Don't be!! I have done this on a regular basis. Bryan ----- Original Message ----- From: "UniPath IHC" To: Sent: Monday, July 19, 2004 3:57 PM Subject: [Histonet] Congo Red > Has anyone ever heard of doing an overnight Congo Red (overnight in the > Congo Red soln.)? One of our Paths is requesting this and we're worried > about false positive staining. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AFeatherstone <@t> KaleidaHealth.Org Mon Jul 19 15:28:46 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] lymph node clearing, hardening Message-ID: Anyone using a homemade lymph node clearing and hardening solution that does not contain chloroform or ether? Annette Featherstone -----Original Message----- From: Stephen.Eyres@sanofi-synthelabo.com [mailto:Stephen.Eyres@sanofi-synthelabo.com] Sent: Monday, July 19, 2004 09:05 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bouin's alternative Hi Marlene, We use Davidson's fluid, which is a mixture of Alcohol, formalin and acetic acid. Steve Marlene Nardi To: histonet@lists.utsouthwestern.edu Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] Bouin's alternative western.edu 19/07/2004 13:17 We currently use Bouin's solution to fix animal eyes (good retinal preservation), to fix animal reproductive tissues (prevents shrinkage of testes), and as the post-fix in the Masson Trichrome stain. Any ideas on a less hazardous alternative solution for any of these uses? For the Trichrome stain I have tried the commercial Bouin's substitute, Gram's iodine and citrate buffer with limited/no success. thank you in advance, Marlene ===== Marlene C. Nardi Technical Services Supervisor Histology Laboratory New York State College of Veterinary Medicine Cornell University Department of Biomedical Sciences S2-124 Schurman Hall Ithaca, NY 14853 mailto:mcc12@cornell.edu ph. 607-253-3314 or 607-253-3303 FAX 607-253-3357 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------- Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu?il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d?en aviser imm?diatement l?exp?diteur par retour de courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuelles pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ---------------------------------------------------------- CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From hfaraji73 <@t> hotmail.com Mon Jul 19 15:39:57 2004 From: hfaraji73 <@t> hotmail.com (hamidreza faraji) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] (no subject) Message-ID: Please do not send me any messages. thanks _________________________________________________________________ Free yourself from those irritating pop-up ads with [1]MSN Premium: Join now and get the first two months FREE* References 1. http://g.msn.com/8HMAENCA/2752??PS=47575 From convmcm <@t> cc.usu.edu Mon Jul 19 17:11:42 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Congo Red In-Reply-To: <000001c46dca$95752400$4500a8c0@unipath02> Message-ID: <001901c46ddd$621b41d0$4a737b81@Cygnus> I just made up fresh congo red solutions today... I wonder if he is thinking of how you need to let the congo red stock soln stand overnight??? I have two procedures, both are only 20 minutes in the working solution. I'm not much help, am I? *g* Connie McManus, who is wondering: Why am I in this handbasket and where am I going???? Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of UniPath IHC Sent: Monday, July 19, 2004 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Congo Red Has anyone ever heard of doing an overnight Congo Red (overnight in the Congo Red soln.)? One of our Paths is requesting this and we're worried about false positive staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Hanne.Onnasch <@t> agriculture.gov.ie Tue Jul 20 03:59:11 2004 From: Hanne.Onnasch <@t> agriculture.gov.ie (Onnasch, Hanne) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] GFAP Protocol Message-ID: Hi All, I'm looking for a protocol for IHC staining for GFAP on paraffin sections. Does anyone have experience with using the Dako detection kit and an automated system? Regards, Hanne Hanne Onnasch Research Officer Central Veterinary Research Laboratory Abbotstown Castleknock Dublin 15 phone: +353(0)16072583 Fax: +353(0)16072604 hanne.onnasch@agriculture.gov.ie ********************************************************************** *********** Department of Agriculture and Food *************** The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). ********************************************************************** From rentonlf <@t> bru.wits.ac.za Tue Jul 20 06:24:06 2004 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] in-vivo imaging techniques Message-ID: <1090322646.96fb10c0rentonlf@bru.wits.ac.za> Maria, This is just a shot in the dark but.... Is it possible to make up a concentrated stock solution (maybe keep it frozen in microlitre amounts) which you can then dilute to the required concentration? Then you don't have to weigh out such small quantities each time. What about asking the supplier if they couldn't dispense smaller amounts for you? Hello Everyone, I posted this message last week (and) I have yet to receive any responses. So, I've decided to send the message...again. In hopes of something. This time my message is a bit more detailed. Keep in mind that the company who make the dye (they have the patent), isn't more helpful. Please, can anyone help, especially the neuroscience folks? One of the PI's I work with is interested in in-vivo imaging techniques. We've been trying for sometime (w/out consist success) to use a particular blue voltage sensitive dye called RH-1692. This type of imaging is a hot new area!! The dye compound is used for low biological noise probing for high-speed in-vivo neural imaging of electrical signals. The problem is in the dye preparation. You wouldn't think this would be a problem, but it is!! I have to weigh very tiny amounts at a time (1mg), the compound is very sticky (and) it's also hygroscopic - sucks up water from the air, thus gets more sticky!! Compound is also light sensitive, so I wrap the vial(s) in foil and work in very dim room. When not is use the dye is stored @ 4 degrees and when used is mixed with either home made or commercial artificial cerebrospinal fluid (CSF) and heated to about 50 F degrees to dissolve. Did I mention that its not easy to dissolve; we've used heating the mixed solution, sonicating it to help it dissolve faster and also have tried using less heat...nothing! The good (but frustrating) news is that it did work twice (2) experiments of of six (6). Which leads us to the possibility that the stock dye might be suspect. We've also used dye with different lot numbers, same results...inconsistancy. So, unless we get some specific details on this dye , hopefully from neuro folks who are generous in sharing information technique and who I can contact directly, we are faced with the possibility, but an expensive fact that we might have to order a new dye vial for each experiment! Sorry for being lengthy. Any information, suggestions, tips...anything anyone can provide, I would greatly appreciate it and look forward to hearing from you. most sincerely, Maria Mejia neurohistologist Smith-Kettlewell Eye Research Insititute San Francisco, CA Email: maria@ski.org Phone: (415)-345-2185/2165 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From AFeatherstone <@t> KaleidaHealth.Org Tue Jul 20 07:10:21 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] GFAP Protocol Message-ID: I have not used Dako but we always use citrate buffer ph6.0 in steamer for antigen retrieval. Always works! Annette Featherstone HT/MLT -----Original Message----- From: Onnasch, Hanne [mailto:Hanne.Onnasch@agriculture.gov.ie] Sent: Tuesday, July 20, 2004 04:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GFAP Protocol Hi All, I'm looking for a protocol for IHC staining for GFAP on paraffin sections. Does anyone have experience with using the Dako detection kit and an automated system? Regards, Hanne Hanne Onnasch Research Officer Central Veterinary Research Laboratory Abbotstown Castleknock Dublin 15 phone: +353(0)16072583 Fax: +353(0)16072604 hanne.onnasch@agriculture.gov.ie ********************************************************************** *********** Department of Agriculture and Food *************** The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From shive003 <@t> umn.edu Tue Jul 20 08:03:19 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] GFAP Protocol References: Message-ID: <002801c46e59$edb14920$78065486@vdl220FAC> Sure. I use Dako's GFAP on their autostainer all the time in veterinary diagnostics. It requires NO tissue pretreatment. The antibody can be used at a very high dilution (right now I'm using 1:1200 dilution of anti-GFAP with a DAKO EnVision+/Rb detection system). Jan Shivers University of Minnesota Veterinary Diagnostic Lab St. Paul, MN, USA 612-624-7297 shive003@tc.umn.edu ----- Original Message ----- From: "Onnasch, Hanne" To: Sent: Tuesday, July 20, 2004 3:59 AM Subject: [Histonet] GFAP Protocol Hi All, I'm looking for a protocol for IHC staining for GFAP on paraffin sections. Does anyone have experience with using the Dako detection kit and an automated system? Regards, Hanne Hanne Onnasch Research Officer Central Veterinary Research Laboratory Abbotstown Castleknock Dublin 15 phone: +353(0)16072583 Fax: +353(0)16072604 hanne.onnasch@agriculture.gov.ie ********************************************************************** *********** Department of Agriculture and Food *************** The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Tue Jul 20 08:37:17 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Re: Costong for TMA Message-ID: Hi, You should check out the new Journal of Histotechnology. There's an article about TMA's that answers your questions. Jo >>> "Histo Jock" 07/17/04 05:42PM >>> There's probably too many factors involved to make a decent estimate. Are you using tape transfer or a water bath? What size punches are you using? How experienced is the tech? etc. etc. etc. I've seen techs take a perfect section in a few seconds, others that take minutes to get a decent cut. It's all dependant on your individual setup. HistoJock. >------------------------------ > >From: "Angela Bitting" >Subject: [Histonet] Costing for TMA > >Has anyone calculated the tech time it takes to cut slides from TMA blocks? _________________________________________________________________ Planning a family vacation? Check out the MSN Family Travel guide! http://dollar.msn.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jul 20 09:08:49 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Fixative and H2O2 for frozen In-Reply-To: References: Message-ID: <6.0.0.22.0.20040720080351.01b47570@gemini.msu.montana.edu> This fixative is for air dried frozen sections (overnight dry at room temperature), fix 5 minutes. I have reused the fixative, but if you have a lot of humidity or fix a large number of slides, keep reuse time short, maybe a week. I do store used fixative in a sealed bottle. I NEVER use hydrogen peroxide in PBS, I use DAKO S2001 peroxidase block for minimally fixed frozen sections. This is made up in PBS, 0.03% hydrogen peroxide and some sodium azide. IF you get your hydrogen peroxide concentration too high you will chew your sections off the slides. This block is gentle for frozen sections. I can make this up per publication method and I make up a supply for one week then discard the remainder. At 03:52 PM 7/17/2004, you wrote: >I do use freshly prepared fixative and h202 each time. >Patsy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jorge >Ivan Zapata Valencia >Sent: Saturday, May 15, 2004 12:46 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Fixative and H2O2 for frozen > > >Hello everybody >a technical question: may I use the same acetone:etanol mix to fix samples >different days, or do I have to prepare it feshly every day?? The same with >the H2O2 PBS solution for endogenous peroxidase blocking. Is it mandatory to >use fresh one? >Thank You very much > >jorge ivan zapata Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Tue Jul 20 09:12:29 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Fixation and Decalcification of Bone Marrow Bxs. In-Reply-To: References: <4FE843CBBAC1D211A8150008C70952DAAE7E01@hk01nt05.hkh.wgkk.sozvers.at> Message-ID: <6.0.0.22.0.20040720081020.01b6b838@gemini.msu.montana.edu> Rather than use a combination fixative and decalcifier (Formalin with EDTA) fix the biopsy THEN do the decalcification. Remember that EDTA will chelate metal ions, may not be so good with zinc!?? At 03:52 PM 7/17/2004, you wrote: >Alex, >Try using zinc formalin in place of your NBF with the EDTA, it should >improve your H&E morphology. >Patsy From blaesse <@t> rhrk.uni-kl.de Tue Jul 20 09:19:24 2004 From: blaesse <@t> rhrk.uni-kl.de (Peter Blaesse) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Plasma membrane marker Message-ID: I am looking for an antibody against a plasma membrane marker protein for IHC (brainstem slices). The antibody should work after fixation with Zamboni?s solution (2% PFA, 15% picrinic acid (aqueous solution)). This fixation is optimal for the staining of my protein of interest. So, I do not want to change the protocol. The antibody against my protein of interest is from rabbit. Does anibody know an antibody that is suitable? Thanks for your help. peter From dcrippen <@t> buckinstitute.org Tue Jul 20 09:55:10 2004 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] IHF with blue conjugated secondaries Message-ID: <4AA34A707932424EBE2D973764D226A90158EEEB@inverness.buckcenter.org> Dear Gayle and All, Thank you so very much for all the support around this issue. I will keep plugging this out and if successful will post the solution to the list. Something to note for those who share my blue "blues" is that both Jackson ImmunoResearch and Molecular Probes support techs assure me that this is not an uncommon problem. With MANY thanks! Danielle Crippen Morphology Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org -----Original Message----- From: Danielle Crippen Sent: Wednesday, July 14, 2004 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHF with blue conjugated secondaries Dear All, I have an investigator who is in need of triple fluorescence labeling on mouse brain tissue. The green fluorophore is injected and the request is that we double label for two additional antigens. Our problem is that we cannot find a blue conjugated secondary that works at all. We've purchased AMCA Dn x Rb (Jackson) and 350 Dn x Gt (Molecular Probes) and have tried them at every possible working concentration recommended on the spec sheets. We've included GFAP positive controls with Cy3 secondary (this works beautifully) and we've tried using alternate tissue to eliminate the possibility that it's our particular sample. We've also tried varying the blocking method and several different protocols. Considering the confines of our microscope set-up, we must use a blue and a red conjugated secondary. Additionally, one of our primary antibodies is made in goat, so both our secondaries must be made in donkey. Any suggestions as to vendors, protocols or particular references from the literature are VERY welcome!!! Many many thanks in advance!! Danielle Crippen Morphology Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Jul 20 12:59:33 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Plasma membrane marker In-Reply-To: References: Message-ID: <40FD5D85.30405@umdnj.edu> Hi Peter: You will have to tell us what the "protein of interest" is before we, or anyone else, can answer your question. There are companies that will make antibodies to your protein, if nothing is commercially available. Such an antibody could be tagged with a marker for microscopy, if desired. How do you know that Zamboni's is the best fixative if you are still looking for an antibody to your "protein of interest"? Geoff Peter Blaesse wrote: >I am looking for an antibody against a plasma membrane marker protein for >IHC (brainstem slices). The antibody should work after fixation with >Zamboni?s solution (2% PFA, 15% picrinic acid (aqueous solution)). This >fixation is optimal for the staining of my protein of interest. So, I do not >want to change the protocol. The antibody against my protein of interest is >from rabbit. Does anibody know an antibody that is suitable? > >Thanks for your help. > >peter > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From azdudley <@t> hotmail.com Tue Jul 20 10:45:01 2004 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] GFAP Protocol Message-ID: dear hanne, I use dako,s gfap abtibody on their automated stainer and results are good. sincerely, anita dudley, providence hosp. mobile alabama >From: "Onnasch, Hanne" >To: >Subject: [Histonet] GFAP Protocol >Date: Tue, 20 Jul 2004 09:59:11 +0100 > > >Hi All, > >I'm looking for a protocol for IHC staining for GFAP on paraffin sections. >Does anyone have experience with using the Dako detection kit and an >automated system? > >Regards, >Hanne > > >Hanne Onnasch >Research Officer >Central Veterinary Research Laboratory >Abbotstown >Castleknock >Dublin 15 > >phone: +353(0)16072583 >Fax: +353(0)16072604 >hanne.onnasch@agriculture.gov.ie > > > > >********************************************************************** >*********** Department of Agriculture and Food *************** > >The information contained in this email and in any >attachments is confidential and is designated solely >for the attention and use of the intended recipient(s). >This information may be subject to legal and professional >privilege. If you are not an intended recipient of >this email, you must not use, disclose, copy, >distribute or retain this message or any part of it. >If you have received this email in error, please >notify the sender immediately and delete all copies of >this email from your computer system(s). >********************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ MSN 9 Dial-up Internet Access helps fight spam and pop-ups – now 2 months FREE! http://join.msn.click-url.com/go/onm00200361ave/direct/01/ From sa.drew <@t> hosp.wisc.edu Tue Jul 20 11:16:20 2004 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] PML Message-ID: Now that our finite stock of home-brew PML antibody is diminishing, we would appreciate it if others would share their opinion on their favored vendor for this antibody. We are running our cases on the Ventana line of automated stainers... Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From blaesse <@t> rhrk.uni-kl.de Tue Jul 20 11:09:38 2004 From: blaesse <@t> rhrk.uni-kl.de (Peter Blaesse) Date: Fri Sep 16 15:23:45 2005 Subject: AW: [Histonet] Plasma membrane marker In-Reply-To: <40FD5D85.30405@umdnj.edu> Message-ID: Hi Geoff, my message was capable of being misunderstood. Sorry for that. I have an antibody against my protein of interest (rabbit-anti-KCC2; KCC2 - a potassium-chloride-cotransporter). I need a plasma membrane marker to do co-stainings with anti-KCC2. So, the antibody against the marker protein shouldn?t be a rabbit-ab. peter > -----Urspr?ngliche Nachricht----- > Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] > Gesendet: Dienstag, 20. Juli 2004 20:00 > An: Peter Blaesse > Cc: histonet@lists.utsouthwestern.edu > Betreff: Re: [Histonet] Plasma membrane marker > > > Hi Peter: > > You will have to tell us what the "protein of interest" is before > we, or anyone else, can answer your question. > There are companies that will make antibodies to your protein, if > nothing is commercially available. Such an antibody could be tagged with > a marker for microscopy, if desired. > How do you know that Zamboni's is the best fixative if you are still > looking for an antibody to your "protein of interest"? > > Geoff > > Peter Blaesse wrote: > > >I am looking for an antibody against a plasma membrane marker protein for > >IHC (brainstem slices). The antibody should work after fixation with > >Zamboni?s solution (2% PFA, 15% picrinic acid (aqueous solution)). This > >fixation is optimal for the staining of my protein of interest. > So, I do not > >want to change the protocol. The antibody against my protein of > interest is > >from rabbit. Does anibody know an antibody that is suitable? > > > >Thanks for your help. > > > >peter > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > From pruegg <@t> ihctech.net Tue Jul 20 12:25:25 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] adhesive tape & MMA Sections In-Reply-To: <1089639857.8cf75d00rentonlf@bru.wits.ac.za> Message-ID: Louise, I have done some work with this and never found it very satisfing. Why did you stick the sections onto office tape. Instrumedics has a tape transfer system using their tape and polymer coated slides which the tape section rolls onto and then is exposed to uv light, the section is polymerized to the coated slide and then you can remove the tape after soaking in a turpentine solovent. With what you already have I would do all the staining on the tape and then try and stick it to a slide at the end, maybe leaving the tape on the section, soak it in xylene briefly and coverslip sort of using the tape as your glass cover, or if it is not too think and wrinkled use permount and a glass coverslip to attach it to a slide. This has always been a mess in my hands, the adhesive stains and everything wrinkles and bubbles up. Patsy -----Original Message----- From: renton louise mrs [mailto:rentonlf@bru.wits.ac.za] Sent: Monday, July 12, 2004 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] adhesive tape & MMA Sections OK, this is the last time I'm posting this, so if this is version #54, please bear with me, as it seems some sort of gremlin crept into the system. The question is: How do I treat MMA sections that are stuck onto common office type sticky tape? Do I: a) Stain first & stick onto a slide later -if so what type of adhesive will work best? b)Stick down first & remove sticky tape ( what solvent to try I'm scared I'll lose the section altogether) c)Stain, stick down & ignore the sticky tape & mount as usual. Any & all help will be appreciated (but please don't ask me to consider the Instrumedics system - its not appropriate for this application) Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From pruegg <@t> ihctech.net Tue Jul 20 12:56:53 2004 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] new email address for Patsy Message-ID: I have a new email address, it is pruegg@ihctech.net and a web site soon to come www.ihctech.net I still have my other email address pruegg@msn.com as well. Patsy Ruegg IHCtech, LLC 12635 Montview Blvd. Suite 216 Aurora, CO 80010 720-859-4060 From gcallis <@t> montana.edu Tue Jul 20 13:35:44 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] ] Gold Chloride for Retic In-Reply-To: References: Message-ID: <6.0.0.22.0.20040720122500.01b6d338@gemini.msu.montana.edu> As with all working chemicals, if you exhaust the active ingredient (gold chloride molecules) it will NOT work properly. Set an expiration date for your gold chloride, the working solution. OR better yet, set the number of slides going through this at 50, then make up new. If you start to get reddish color, then reduce the number of slides going through 50 mls working solution. Concentrated stock solution remains stable for a long time until you make it up working concentration. We store stock in the refrigerator. If something lasted "forever", we would have no problems. We always watched our fibers (where there are the most fibers located) turn dark blackish gray, I know with Grocotts methenamine silver, if you leave sections too long in gold chloride they go to a very violet tinctorial shade, not good - you want blackish-gray. This is the same for Jones methenamine silver for renal biopsies too. The times are often more average for toning, some methods give less time but if your eye lets you control the color development, it helps - run a clock when you do this. At 08:08 AM 7/19/2004, you wrote: >I have heard that if the Gold Chloride is old it can cause a more "reddish" >stain in the retic. Some people are saying it lasts "forever". What is the >general consenus? >Annette Featherstone >HT/MLT > >- From gcallis <@t> montana.edu Tue Jul 20 13:39:51 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] cryostats In-Reply-To: References: Message-ID: <6.0.0.22.0.20040720123747.01b69fc0@gemini.msu.montana.edu> WE have two 1850's side by side and I have NEVER had problems with the blade holder even after two years use with both of them. You need to find out from the Leica rep what is going wrong with your holder, something is out of whack. We cut hundreds of frozen sections a year - in fact, rarely do paraffin for any immunohistochemistry. At 09:31 AM 7/19/2004, you wrote: >I have never used a H/B, but I would give it a try. Try both before you >buy. I have a Leica 1850 and it had to be replaced within two years. The >current one is doing fine. If you get a good one the Leica's are okay >except for the blade holder. It has to be taken apart and adjusted every >time you change the blade( the pressure clip is to soft). Now if you >could choose another company I would try Microm(Richard Allan). > >Juan C. Gutierrez, HT(ASCP) >Histology Laboratory Supervisor >Christus Santa Rosa Hospital >333 N. Santa Rosa Ave. >San Antonio, TX 78207 >(210)704-2533 > > >-----Original Message----- >From: Kathleen Spencer [mailto:kspencer@utmem.edu] >Sent: Monday, July 19, 2004 9:51 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] cryostats > > >Hi all, > >Question: If you had to buy a new cryostat, and you had to chose between >a Hacker Bright or a Leica, which would you purchase, and please tell me >why. Mainly I would love to hear from anyone that has had these in the >last 10 years. >I really need your input on this, detailed input, especially in regards >to when you bought the machine that you do not like and how long before >it started giving you trouble. > >Thanks in advance, > >Kathleen Spencer HT (ASCP) >Lab Manager/ LCM Supervisor >UTHSC > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From adelsyscarol <@t> yahoo.com Tue Jul 20 13:43:06 2004 From: adelsyscarol <@t> yahoo.com (Carol Wilson) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Microtome Knife Sharpening Service Available Message-ID: <20040720184306.60818.qmail@web50603.mail.yahoo.com> We now have microtome knife sharpening capabilities. We can accept knives up to 25cm. The charge is from $35 - $50 plus shipping depending on the size of the knife. If you are interested please contact me directly for further information. Carol Wilson, H.T. (A.S.C.P.) Histology and Laboratory Products and Service Adelsys, Inc. Adelsyscarol@yahoo.com www.adelsys.com --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - Send 10MB messages! From AFeatherstone <@t> KaleidaHealth.Org Tue Jul 20 13:49:28 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] Auramine for Fluoresence Message-ID: Any quik stain for Auramine O Fluorescence for TB? Annette HT/MLT -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Tuesday, July 20, 2004 14:36 To: Featherstone, Annette; Histonet@lists.utsouthwestern.edu Subject: ] Gold Chloride for Retic As with all working chemicals, if you exhaust the active ingredient (gold chloride molecules) it will NOT work properly. Set an expiration date for your gold chloride, the working solution. OR better yet, set the number of slides going through this at 50, then make up new. If you start to get reddish color, then reduce the number of slides going through 50 mls working solution. Concentrated stock solution remains stable for a long time until you make it up working concentration. We store stock in the refrigerator. If something lasted "forever", we would have no problems. We always watched our fibers (where there are the most fibers located) turn dark blackish gray, I know with Grocotts methenamine silver, if you leave sections too long in gold chloride they go to a very violet tinctorial shade, not good - you want blackish-gray. This is the same for Jones methenamine silver for renal biopsies too. The times are often more average for toning, some methods give less time but if your eye lets you control the color development, it helps - run a clock when you do this. At 08:08 AM 7/19/2004, you wrote: >I have heard that if the Gold Chloride is old it can cause a more "reddish" >stain in the retic. Some people are saying it lasts "forever". What is the >general consenus? >Annette Featherstone >HT/MLT > >- CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From dlcowie <@t> prodigy.net Tue Jul 20 14:04:00 2004 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] embedding derms Message-ID: <20040720190400.55301.qmail@web81001.mail.yahoo.com> hi netters, Does anyone know of a good reference book covering embedding procedures for derms? Any info will be appreciated. Thanks in advance, Dawn From joseph-galbraith <@t> uiowa.edu Tue Jul 20 15:33:28 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] cryostats Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008608@uihc-mail1.uihc.uiowa.edu> Juan: Sounds like an adjustment issue on the holder. Not certain what model of blade holder you have but some older Leica models have two screws aligned horizontally one on each side of the locking plate. This style was harder to adjust. We replaced our blade holder on the 1850 with a newer model holder just to get rid of that hassle. The newer models have one central large screw with a tiny adjustment screw immediately behind it. The latter type can be adjusted quite easily to allow smooth blade changes and yet still get a good tight grip. If you let me know what type of holder you have I can probably be more specific about adjusting it. You may also have problems with moisture and ice crystal formation if you are cleaning (disinfecting) the cryostat blade holder while it is cold. Send me some more info and maybe I can help you out. Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Tuesday, July 20, 2004 1:40 PM To: GUTIERREZ, JUAN; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostats WE have two 1850's side by side and I have NEVER had problems with the blade holder even after two years use with both of them. You need to find out from the Leica rep what is going wrong with your holder, something is out of whack. We cut hundreds of frozen sections a year - in fact, rarely do paraffin for any immunohistochemistry. At 09:31 AM 7/19/2004, you wrote: >I have never used a H/B, but I would give it a try. Try both before you >buy. I have a Leica 1850 and it had to be replaced within two years. The >current one is doing fine. If you get a good one the Leica's are okay >except for the blade holder. It has to be taken apart and adjusted every >time you change the blade( the pressure clip is to soft). Now if you >could choose another company I would try Microm(Richard Allan). > >Juan C. Gutierrez, HT(ASCP) >Histology Laboratory Supervisor >Christus Santa Rosa Hospital >333 N. Santa Rosa Ave. >San Antonio, TX 78207 >(210)704-2533 > > >-----Original Message----- >From: Kathleen Spencer [mailto:kspencer@utmem.edu] >Sent: Monday, July 19, 2004 9:51 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] cryostats > > >Hi all, > >Question: If you had to buy a new cryostat, and you had to chose between >a Hacker Bright or a Leica, which would you purchase, and please tell me >why. Mainly I would love to hear from anyone that has had these in the >last 10 years. >I really need your input on this, detailed input, especially in regards >to when you bought the machine that you do not like and how long before >it started giving you trouble. > >Thanks in advance, > >Kathleen Spencer HT (ASCP) >Lab Manager/ LCM Supervisor >UTHSC > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Tue Jul 20 15:43:06 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:45 2005 Subject: [Histonet] GFAP Protocol Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008609@uihc-mail1.uihc.uiowa.edu> Hanne: We use Biogenex GFAP mono (1:1000) and poly (1:150) Ab on Dako autostainer using Envision Plus and 15:15 protocols. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Onnasch, Hanne Sent: Tuesday, July 20, 2004 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GFAP Protocol Hi All, I'm looking for a protocol for IHC staining for GFAP on paraffin sections. Does anyone have experience with using the Dako detection kit and an automated system? Regards, Hanne Hanne Onnasch Research Officer Central Veterinary Research Laboratory Abbotstown Castleknock Dublin 15 phone: +353(0)16072583 Fax: +353(0)16072604 hanne.onnasch@agriculture.gov.ie ********************************************************************** *********** Department of Agriculture and Food *************** The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Tue Jul 20 15:53:14 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Gold Chloride for Retic Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403005B24@uihc-mail1.uihc.uiowa.edu> Annette: Excessive time in gold chloride will produce a reddish brown tone to Retc stained tissue elements rather than the prefered gray/black. In our practice we actually prefer a little overdone gold chloride since we follow our Retc with an H+E and we find the H+E is a bit crisper and brighter with overdone gold chloride. To my knowledge the solution is stable but we replace ours at least every 2 weeks because it gets dirty (dingy) and perhaps the gold would eventually get consumed due to replacement with silver??? Best wishes, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Featherstone, Annette Sent: Monday, July 19, 2004 9:08 AM To: 'Patsy Ruegg'; Tan, MinHan; histonet@pathology.swmed.edu Subject: [Histonet] Gold Chloride for Retic I have heard that if the Gold Chloride is old it can cause a more "reddish" stain in the retic. Some people are saying it lasts "forever". What is the general consenus? Annette Featherstone HT/MLT -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Saturday, July 17, 2004 17:53 To: Tan, MinHan; histonet@pathology.swmed.edu Subject: RE: [Histonet] DAB (DAKO and Vector) I have used Envision with many DAB's including vector, it works fine. My favorite continues to be the Bragatti Stable DAB, but don't buy it from Invitrogen, get it from Phoenix Biologicals it is better quality. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan Sent: Friday, May 14, 2004 12:41 PM To: histonet@pathology.swmed.edu Subject: [Histonet] DAB (DAKO and Vector) Hi, Thanks all who replied to my query on pressure cookers. I have a question for those who have used the Envision + kit. Has anyone used it with the Vector DAB kit, rather than the DAB+ from DAKO? Thanks! Min-han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jul 20 09:23:18 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Bouin's alternative In-Reply-To: References: Message-ID: <6.0.0.22.0.20040720081538.01b8bb38@gemini.msu.montana.edu> Bouins is an integral part of the Massons trichrome stain and even if you fix with Davidsons, you will probably have to post fix the sections with Bouins. You can buy it commercially made up and reuse it many times for this purpose. This way you can fix eyes with another fixative and not have to worry about fixing the tissues in Bouins - but it is important to use it with the trichrome method. I see the panic in people about picric acid, but I think it can be handled safely for a given staining method. I have picric acid stored on my shelf but KEEP IN UNDER WATER at all times, and have had zero problems with Bouins or the picric acid itself. By using Bouins for section post-fixation rather than the large amount for fixing a tissues, you will reduce the total volume of Bouins in lab, which should be a help. We will continue to use Bouins forever, I guess. AND we are not in a huge state of worry about it. Just wipe around lids and never let it get in contact with metal. I have worse, more hazardous chemicals in my lab to worry about as compared to Bouins, sorry - I have found no substitute for it with the Mass Tri stain. From degaboh <@t> rice.edu Tue Jul 20 16:20:00 2004 From: degaboh <@t> rice.edu (ZP) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Blood vessel antibodies for rabbit models In-Reply-To: <20040720172933.701301DB0E@fungible10.mail.rice.edu> Message-ID: <200407202120.i6KLK3Q1021738@ms-smtp-01-eri0.texas.rr.com> Hello all, I'd like to use rack the brains of all you histonetters. Can anyone recommend antibodies to CD31 and/or CD34 that can be used in rabbit models? Or, can anyone recommend other antibodies for blood vessels that can be used in rabbit models? I'm having a hard time finding these. Thanks! Zarana Patel degaboh@rice.edu From ftulenko06 <@t> jcu.edu Tue Jul 20 17:46:29 2004 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] (no subject) Message-ID: Hello histonetters, Does anybody have any experience removing mercury chloride from fixed specimens using lugols solutions and sodium thiosulfate? I wasn't sure how long each specimen should be placed in each of the two washes. Thanks, Frank From scoop <@t> mail.nih.gov Tue Jul 20 21:10:19 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] formalin nomenclature Message-ID: Dear Histoneteers, Can someone please explain to me why some tissue fixation protocols call for 4% paraformaldehyde while others call for 10% formaldehyde and what the difference is? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From debydun <@t> yahoo.com Tue Jul 20 21:55:11 2004 From: debydun <@t> yahoo.com (Debra Dunlap) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Antibody for CYP7A CYP7A1 Message-ID: <20040721025511.67673.qmail@web61003.mail.yahoo.com> Hi All, Can anyone point me to a source for CYP7A or CYP7A1....besides santa cruz? I am going to use the antibody for western blot but an antibody that can be used in IHC would be a bonus. Thank you. --------------------------------- Do you Yahoo!? Vote for the stars of Yahoo!'s next ad campaign! From mbromley <@t> dhm.com.au Tue Jul 20 23:59:12 2004 From: mbromley <@t> dhm.com.au (Mark Bromley) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] formalin nomenclature In-Reply-To: Message-ID: Hi Sharon. Formaldehyde has the formula H2C=O and is a gas at room temperature. If you open up one of the double bonds between the carbon and the oxygen you can get formaldehyde to polymerise forming chains like: -H2C-O-H2C-O-H2C-O- etc, having the general formula (H2C-O)N and it is this polymerised form of formaldehyde which is called paraformaldehyde. It exists as a white powder at room temperature. Neither gaseous formaldehyde or paraformaldehyde powder is much use to a histologist on its own. But when you dissolve either of them in water to the point of saturation, you will find you have a 38% solution of formaldehyde in water. (Paraformaldehyde just dissociates back into individual formaldehyde units when dissolved, so from our point of view, formaldehyde and paraformaldehyde are essentially the same thing. It is this 38% solution of formaldehyde in water that is called Formalin. Formalin = 38% formaldehyde solution. Making up the 10% formalin we routinely use is a matter of a 10 fold dilution of formalin. This 10 fold dilution of a 38% solution of formaldehyde produces a 3.8% solution. So in answer to your question, a 4% (or 3.8% to be exact) paraformaldehyde solution, 4% formaldehyde solution and 10% Formalin are all exactly the same thing. Hope that helped. Mark Bromley, Histology Department, Douglass Hanly Moir Pathology, Sydney, Australia. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sharon Cooperman Sent: Wednesday, 21 July 2004 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin nomenclature Dear Histoneteers, Can someone please explain to me why some tissue fixation protocols call for 4% paraformaldehyde while others call for 10% formaldehyde and what the difference is? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From je22r <@t> udcf.gla.ac.uk Wed Jul 21 02:59:03 2004 From: je22r <@t> udcf.gla.ac.uk (Julia Edgar) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Plasma membrane marker Message-ID: <001801c46ef8$972a40d0$40e9d182@vet> Hi Peter Which cell type do you wish to stain? Neurons or glia? Julia >I am looking for an antibody against a plasma membrane >marker protein for > >IHC (brainstem slices). From blaesse <@t> rhrk.uni-kl.de Wed Jul 21 03:29:38 2004 From: blaesse <@t> rhrk.uni-kl.de (Peter Blaesse) Date: Fri Sep 16 15:23:46 2005 Subject: AW: [Histonet] Plasma membrane marker In-Reply-To: <001801c46ef8$972a40d0$40e9d182@vet> Message-ID: Hi Julia, I would prefer a neuronal marker, but an antibody which stains both should be also fine. I?ve tried some antibodies against Na+-K+-ATPases which we use for immunoblots and they didn?t work for IHC (at least under the conditions I have to use for KCC2 staining). peter > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]Im Auftrag von Julia > Edgar > Gesendet: Mittwoch, 21. Juli 2004 09:59 > An: Histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Plasma membrane marker > > > Hi Peter > Which cell type do you wish to stain? Neurons or glia? > > Julia > > >I am looking for an antibody against a plasma membrane >marker > protein for > > >IHC (brainstem slices). > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jluis.palazon <@t> icman.csic.es Wed Jul 21 04:59:58 2004 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] AURANTIA Message-ID: <20040721095958.6FCA3256A87@perceval.uca.es> Dear Histonet fellows Do any of you know the comertial name of "Aurantia"? I need this reagent but I can?t find it in the chemical product catalogs. thanks in advance Jos? Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es From lpwenk <@t> sbcglobal.net Wed Jul 21 05:49:41 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] AURANTIA References: <20040721095958.6FCA3256A87@perceval.uca.es> Message-ID: <004f01c46f10$6e29a780$a521d445@domainnotset.invalid> By looking on the web, and cross referencing many web pages (several in German (no, don't speak it, but chemistry is chemistry)), could this be what you are looking for? Aurantia CAS number 131-73-7 Name: 2,2?,4,4?,6,6?-Hexanitrodiphenylamine Synonym: Dipicrylamine Formula: C12H5N7O12 C.I.10360 Danger! Material is shock sensitive and potentially explosive. Fisher has a MSDS on it. https://fscimage.fishersci.com/msds/91088.htm That's the only company I could find with an MSDS on this chemical, on the web. However, the Fisher page lists an Acros number. So this chemical might also be in the Acros catalog (I'm at home - no catalogs). Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Jose Luis Palazon Fernandez" To: Sent: Wednesday, July 21, 2004 5:59 AM Subject: [Histonet] AURANTIA Dear Histonet fellows Do any of you know the comertial name of "Aurantia"? I need this reagent but I can?t find it in the chemical product catalogs. thanks in advance Jos? Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jluis.palazon <@t> icman.csic.es Wed Jul 21 06:08:14 2004 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] AURANTIA 2 Message-ID: <20040721110814.5F89D25783A@perceval.uca.es> the reagent I am looking for is used in a staining protocol by J. Gadey for the differentiation of endocrine pancreas cells. many thanks Jos? Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jul 21 07:04:55 2004 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From JWEEMS <@t> sjha.org Wed Jul 21 07:11:26 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: I would check in a local pharmacy. They often have these oils used for making candy. Good luck! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dorothy.L.Webb@HealthPartners.Com Sent: Wednesday, July 21, 2004 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of wintergreen Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Terry.Marshall <@t> rothgen.nhs.uk Wed Jul 21 07:09:36 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: Sorry I can't help with a US supply Dorothy, but can't help the comment that I would prefer the stink of a body to that of oil of Wintergreen. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 21 July 2004 13:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of wintergreen Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rentonlf <@t> bru.wits.ac.za Wed Jul 21 07:33:36 2004 From: rentonlf <@t> bru.wits.ac.za (renton louise mrs) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: <1090413216.96f67700rentonlf@bru.wits.ac.za> Isn't this the stuff that is put in inhalants to clear blocked noses?. There's a rub here called Vicks that has it in as well, either way the pharmacy is the easiest option -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com To: histonet@lists.utsouthwestern.edu Date: Wed, 21 Jul 2004 07:04:55 -0500 Subject: [Histonet] Oil of wintergreen Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? From mab70 <@t> medschl.cam.ac.uk Wed Jul 21 08:05:05 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138A@mius2.medlan.cam.ac.uk> I had to laugh at your comments, Terry, I guess you are right. Isn't oil of wintergreen methyl salicylate or something of the sort? Older chemistry books or the Merck Index should tell us the correct chemical name and then it should be easy to source. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, July 21, 2004 1:10 PM To: Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Oil of wintergreen Sorry I can't help with a US supply Dorothy, but can't help the comment that I would prefer the stink of a body to that of oil of Wintergreen. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 21 July 2004 13:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of wintergreen Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Jul 21 08:01:56 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: Yes it is methyl salicylate. It works in Vick because when you breath it in, you choke and breath deeper:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Margaret Blount [mailto:mab70@medschl.cam.ac.uk] Sent: 21 July 2004 14:05 To: Marshall Terry Dr, Consultant Histopathologist; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Oil of wintergreen I had to laugh at your comments, Terry, I guess you are right. Isn't oil of wintergreen methyl salicylate or something of the sort? Older chemistry books or the Merck Index should tell us the correct chemical name and then it should be easy to source. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, July 21, 2004 1:10 PM To: Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Oil of wintergreen Sorry I can't help with a US supply Dorothy, but can't help the comment that I would prefer the stink of a body to that of oil of Wintergreen. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 21 July 2004 13:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of wintergreen Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madaryhtl <@t> juno.com Wed Jul 21 08:19:14 2004 From: madaryhtl <@t> juno.com (madaryhtl@juno.com) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Needa recycler, could CBG rep call me? Message-ID: <20040721.061958.1935.18115@webmail13.lax.untd.com> I have no email yet nut it will be MadaryJ@medimmune.com. Lab phone is 301.398.6113. Office when it gets hooked up will be 301.398.4745(I think). From robert.davis <@t> spcorp.com Wed Jul 21 08:25:26 2004 From: robert.davis <@t> spcorp.com (Davis, Robert) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Plastics (bones) Message-ID: <4508920F80C0D411B90200508BF9A9F4462255@LAFMSG30.us.schp.com> Does anyone have a procedure for doing bones in plastics. Thanks, RD ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From darkdaym <@t> mindspring.com Wed Jul 21 08:37:04 2004 From: darkdaym <@t> mindspring.com (Mark Ray) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen In-Reply-To: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138A@mius2.medlan.cam.ac.uk> References: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138A@mius2.medlan.cam.ac.uk> Message-ID: <40FE7180.3020304@mindspring.com> Margaret Blount wrote: >I had to laugh at your comments, Terry, I guess you are right. Isn't oil of >wintergreen methyl salicylate or something of the sort? Older chemistry >books or the Merck Index should tell us the correct chemical name and then >it should be easy to source. > >Margaret > >Margaret Blount >Chief Technician >Clinical Biochemistry >University of Cambridge >Addenbrooke's Hospital >Hills Road >Cambridge >CB2 2QR > >-----Original Message----- >From: Marshall Terry Dr, Consultant Histopathologist >[mailto:Terry.Marshall@rothgen.nhs.uk] >Sent: Wednesday, July 21, 2004 1:10 PM >To: Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Oil of wintergreen > > >Sorry I can't help with a US supply Dorothy, but can't help the comment that >I would prefer the stink of a body to that of oil of Wintergreen. > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Dorothy.L.Webb@HealthPartners.Com >[mailto:Dorothy.L.Webb@HealthPartners.Com] >Sent: 21 July 2004 13:05 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Oil of wintergreen > > > >Does anyone know where oil of wintergreen can be purchased? The PA's at our >facility used to use this product in the morgue to take care of odors while >working on an autopsy. I cannot locate the product and was wondering if >anyone could help me or what else is recommended? This is not an area that >I am familiar with and am fairly new to my position here!! Thanks ahead of >time for any and all help!! > >Dorothy Webb >Regions Hospital >St.Paul, Mn. > >________________________________________ > >This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail >is strictly prohibited. > >If you have received this e-mail in error, please immediately notify >the HealthPartners Support Center by telephone at (952) 967-6600. >You will be reimbursed for reasonable costs incurred in notifying us. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Right you are, Margaret. Methyl Salicylate is Synthetic Oil of Wintergreen and is suitable for Dorothy's needs. EK Industries carries it. 800 283 4244. Mark Ray EK Industries From FreidaC <@t> aol.com Wed Jul 21 08:40:30 2004 From: FreidaC <@t> aol.com (FreidaC@aol.com) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] formalin nomenclature Message-ID: <1cc.2651f541.2e2fcc4e@aol.com> The primary difference is that commercial 37-40% formaldehyde solutions contain some methanol; whereas, the paraformaldehyde solutions do not. This has been considered an important difference especially for electron microscopy, and possibly some other applications. Formalin solutions prepared from paraformaldehyde are purer solutions, but unless you need the purity, they are much more difficult to prepare. Freida Carson From Barry.R.Rittman <@t> uth.tmc.edu Wed Jul 21 09:06:52 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0E2DAF9@UTHEVS3.mail.uthouston.edu> Dorothy Hi I must agree that Methyl Salicylate does not do much for ones social life, however would you let us know what you are planning to use this for? I am not sure from your email if you plan to use this to mask odors in the autopsy room or are using this as a clearing agent. Despite its smell it is excellent for clearing blocks and sections. It is however expensive and there are alternatives for both applications. I do not have any catalogues with me at the moment but think that it should be readily available from Fisher and other general chemical companies. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy.L.Webb@HealthPartners.Com Sent: Wednesday, July 21, 2004 7:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of wintergreen Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Chash <@t> reg2.health.nb.ca Wed Jul 21 09:06:36 2004 From: Chash <@t> reg2.health.nb.ca (Sharon Chase) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] p27 antibody Message-ID: I am having problems with the monoclonal p27 antibody. I use super sensitive strepavadin/biotin kit with citrate antigen retrieval. It is staining normal neurons. I contracted the manufacture and they suggested to use an non avidin\biotin kit. I found the non specific staining weaker and if I increase the dilution, the specific staining was lost. If anyone has any suggestions, I would appreciate. From Barry.R.Rittman <@t> uth.tmc.edu Wed Jul 21 09:17:33 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] formalin nomenclature Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0E2DB00@UTHEVS3.mail.uthouston.edu> While formalin solutions contain methanol, it is usually present in around 5% concentration. It is there to act as a reducing agent so that formalin does not become oxidized to formic acid. Once 10% formalin solution is made from the stock formalin solution, the concentration of the methanol is negligible and I do not believe would interfere with the fixing effect of the formalin. The use of phosphate buffering solution essentially eliminates the effects of any formic acid. As Freida has pointed out it is a pain to have to make solution up from scratch, dealing with paraformaldehyde powder etc. If expense is a secondary consideration, and especially for electron microscopy, some companies (I believe Ladd) sell ampoules of pure formalin that is sealed under nitrogen and you merely have to break open the vial and dilute. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FreidaC@aol.com Sent: Wednesday, July 21, 2004 8:41 AM To: scoop@mail.nih.gov; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] formalin nomenclature The primary difference is that commercial 37-40% formaldehyde solutions contain some methanol; whereas, the paraformaldehyde solutions do not. This has been considered an important difference especially for electron microscopy, and possibly some other applications. Formalin solutions prepared from paraformaldehyde are purer solutions, but unless you need the purity, they are much more difficult to prepare. Freida Carson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Jul 21 09:44:16 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] re oil of wintergreen Message-ID: <004501c46f31$32820940$e8345c9f@Carlos> You're right , Margaret. We used to use Methyl salicylate( oil of wintergreen) to process dental tissue. It's still recommended by some molbiol people for clearing wholemounts.Still trying to get the smell out of my clothes ;-) From Bauer.Karen <@t> mayo.edu Wed Jul 21 09:46:45 2004 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Automatic Slide Labelers Message-ID: <8C6E05FA69571948B461F1327CBB893E0629F0DE@lmmail2.ad.lmmhs.org> Hello Histonet, I would like some feedback on automatic slide labelers. I wish to know what people are using and the pros and cons of certain brands. I would be interested in one that's adaptable to our CoPath system and not too large. We have space issues, but could squeeze one in next to our accessioning computer. Got one that you love? I'd really appreciate your feedback. Thanks in advance... Karen Bauer HT(ASCP) Histology Supervisor Department of Pathology Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From leahcox27 <@t> yahoo.com Wed Jul 21 09:51:55 2004 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen In-Reply-To: Message-ID: <20040721145155.21830.qmail@web50207.mail.yahoo.com> I've seen that oil in natural food stores and some vitamin stores. If you live near one that sells tinctures and oils, the clerk there can help you. Also, if you do a search for it on line, a whole bunch of internet retail sites sell it. Leah Dorothy.L.Webb@HealthPartners.Com wrote: Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Vote for the stars of Yahoo!'s next ad campaign! From BWinters <@t> NCH.ORG Wed Jul 21 10:52:00 2004 From: BWinters <@t> NCH.ORG (Winters, Bert) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] DECONTAMINATING CRYOSTATS Message-ID: <270614B321ACB44D8C1D91F4F921FDC3627774@NCH01EX02.nch.org> Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital From histophilhuff <@t> yahoo.com Wed Jul 21 10:52:28 2004 From: histophilhuff <@t> yahoo.com (Phillip Huff) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] adhesive tape & MMA Sections - Turpentine? In-Reply-To: Message-ID: <20040721155228.3768.qmail@web50306.mail.yahoo.com> Hello Patsy, We are currently using the Instrumedic tape-transfer system in our lab. I was surprised to see that you are using turpentine to remove the tape from your slides! We follow the procedure as recommended by Instrumedics: once the section is on the tape and placed in the slide, we UV-flash the tape-section-slide sandwich and place in the back of the cryostat to get very cold. After about 10 minutes, the tape peels off and the section remains in the slide. When and why did you begin using turpentine, and was this a recommendation from Instrumedics? Just curious, Phil Patsy Ruegg wrote: Louise, I have done some work with this and never found it very satisfing. Why did you stick the sections onto office tape. Instrumedics has a tape transfer system using their tape and polymer coated slides which the tape section rolls onto and then is exposed to uv light, the section is polymerized to the coated slide and then you can remove the tape after soaking in a turpentine solovent. With what you already have I would do all the staining on the tape and then try and stick it to a slide at the end, maybe leaving the tape on the section, soak it in xylene briefly and coverslip sort of using the tape as your glass cover, or if it is not too think and wrinkled use permount and a glass coverslip to attach it to a slide. This has always been a mess in my hands, the adhesive stains and everything wrinkles and bubbles up. Patsy -----Original Message----- From: renton louise mrs [mailto:rentonlf@bru.wits.ac.za] Sent: Monday, July 12, 2004 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] adhesive tape & MMA Sections OK, this is the last time I'm posting this, so if this is version #54, please bear with me, as it seems some sort of gremlin crept into the system. The question is: How do I treat MMA sections that are stuck onto common office type sticky tape? Do I: a) Stain first & stick onto a slide later -if so what type of adhesive will work best? b)Stick down first & remove sticky tape ( what solvent to try I'm scared I'll lose the section altogether) c)Stain, stick down & ignore the sticky tape & mount as usual. Any & all help will be appreciated (but please don't ask me to consider the Instrumedics system - its not appropriate for this application) Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa .......so what IS the speed of dark? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Wed Jul 21 11:08:21 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen In-Reply-To: References: Message-ID: <6.0.0.22.0.20040721100709.01ad0128@gemini.msu.montana.edu> Try a pharmacy, where they mix prescriptions for people. Some pharmacies have custom prescription mixture capabilities. Good luck At 06:04 AM 7/21/2004, you wrote: >Does anyone know where oil of wintergreen can be purchased? The PA's at our >facility used to use this product in the morgue to take care of odors while >working on an autopsy. I cannot locate the product and was wondering if >anyone could help me or what else is recommended? This is not an area that >I am familiar with and am fairly new to my position here!! Thanks ahead of >time for any and all help!! > >Dorothy Webb >Regions Hospital >St.Paul, Mn. > >________________________________________ > >This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail >is strictly prohibited. > >If you have received this e-mail in error, please immediately notify >the HealthPartners Support Center by telephone at (952) 967-6600. >You will be reimbursed for reasonable costs incurred in notifying us. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Jul 21 11:10:31 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] ] Gold Chloride for Retic Message-ID: I was almost remembering preparing a colloidal gold solution, was it acid that you added to a gold solution until it was a particular red colour before adding an antibody and adsorbing it to the particles. So does the colour reflect the particle size, I wondered. ( interferred may be a better word than "reflect" ) -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 20 July 2004 19:36 To: Featherstone, Annette; Histonet@lists.utsouthwestern.edu Subject: [Histonet] ] Gold Chloride for Retic As with all working chemicals, if you exhaust the active ingredient (gold chloride molecules) it will NOT work properly. Set an expiration date for your gold chloride, the working solution. OR better yet, set the number of slides going through this at 50, then make up new. If you start to get reddish color, then reduce the number of slides going through 50 mls working solution. Concentrated stock solution remains stable for a long time until you make it up working concentration. We store stock in the refrigerator. If something lasted "forever", we would have no problems. We always watched our fibers (where there are the most fibers located) turn dark blackish gray, I know with Grocotts methenamine silver, if you leave sections too long in gold chloride they go to a very violet tinctorial shade, not good - you want blackish-gray. This is the same for Jones methenamine silver for renal biopsies too. The times are often more average for toning, some methods give less time but if your eye lets you control the color development, it helps - run a clock when you do this. At 08:08 AM 7/19/2004, you wrote: >I have heard that if the Gold Chloride is old it can cause a more "reddish" >stain in the retic. Some people are saying it lasts "forever". What is the >general consenus? >Annette Featherstone >HT/MLT > >- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Jul 21 11:33:07 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] adhesive tape & MMA Sections - Turpentine? In-Reply-To: <20040721155228.3768.qmail@web50306.mail.yahoo.com> References: <20040721155228.3768.qmail@web50306.mail.yahoo.com> Message-ID: <6.0.0.22.0.20040721102109.01b60f18@gemini.msu.montana.edu> We have used the Scotch 3M packaging tape #3560 for picking up paraffin sections from the block. Going to a gelatin subbed slide, and rolled it on to get rid of water from water bath, clamped the slides together with plastic between slide, overnight at 37 then overnight at 60C. To remove this tape, one simply puts it through 3 xylene stations to remove tape easily and any residual glue. This method was published by Diane Sterchi in J of Histotechnology. There are different tapes available, simply trying various brands will tell you is the tape will come off in xylene and leave section on the slide. This has worked with human femoral bone sections and also with difficult eyes that want to come apart on a waterbath. Somewhat fun to do - you have little sections hanging around on edges until you float them on hotter waterbath, pickup, roll out water, trim, clamp and dry. You can pick up PMMA sections the same way by applying tape to block, sectioning. The hard part will be to get the PMMA embedded bone section to stick to a gelatin coated slide. I suggest using 275 or 300 bloom in chrome gelatin mixture and presubbing the slides. Room temperature xylene should let tape release easily, it may soften the PMMA but that is usually better removed with warm xylene. Once the tape is off, bone section is on slide, then you can remove the PMMA with warm xylene. Instrumedics tape transfer is as Phillip described. I have not heard of a solvent used to remove tape, unless this is a unique modification of procedure. From richalarson <@t> yahoo.com Wed Jul 21 11:34:56 2004 From: richalarson <@t> yahoo.com (Richard Larson) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Job search Message-ID: <20040721163456.31327.qmail@web50503.mail.yahoo.com> Hello - I'm a histotech in search of a job. I've been out of the field for three years. I have four years of experience doing routine histology and I have my HT. I am primarily interested in the New England area but I am willing to relocate for the right job. If anyone hears of a job or if there are any other job lists besides the NSH list, I'd be grateful to know. Thanks for your help. Richard Larson __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From JNocito <@t> Pathreflab.com Wed Jul 21 11:48:36 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Disposing of expired IHC reagents: Survey Message-ID: Hey 'netters, Need a little info if you please. How are you disposing of expired primary antibodies and detection reagents? In the trash? Biohazard? Drain? Big bonfire? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From Jason.Wiese <@t> med.va.gov Wed Jul 21 11:02:02 2004 From: Jason.Wiese <@t> med.va.gov (Jason.Wiese@med.va.gov) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: Wintergreen Oil can be purchased from Lorann Oils, Inc. 4518 Aurelius Lansing, MI 48910 Or do a search for Lorann Gourmet... Sorry no phone number... Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Leah Cox [mailto:leahcox27@yahoo.com] Sent: Wednesday, July 21, 2004 7:52 AM To: Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil of wintergreen I've seen that oil in natural food stores and some vitamin stores. If you live near one that sells tinctures and oils, the clerk there can help you. Also, if you do a search for it on line, a whole bunch of internet retail sites sell it. Leah Dorothy.L.Webb@HealthPartners.Com wrote: Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Vote for the stars of Yahoo!'s next ad campaign! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From al.floyd <@t> juno.com Wed Jul 21 11:58:11 2004 From: al.floyd <@t> juno.com (Alton D. Floyd) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] ] Gold Chloride for Retic Message-ID: <20040721.125814.-279241.1.al.floyd@juno.com> Hello David, The procedure you are remembering is the generation of gold colloids for the purpose of performing immunostaining on plastic sections for electron microscopy. And you are correct, the color of the scattered light is indicative of the particle size. Not absolute, since individual eyes vary in color perception so much. Actual size generated depends on the conditions employed in manufacturing the good particles, which are then cleaned by ultracentrifugation prior to use. The colloid itself is generated by the protein coating (which can contain the primary antibodies) which is applied to the particles. Al Floyd 23126 South Shore Drive Edwardsburg, MI 49112 (269) 699-7182 phone & fax From TJJ <@t> Stowers-Institute.org Wed Jul 21 12:11:08 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] adhesive tape & MMA Sections - Turpentine? Message-ID: Patsy was describing the technique for using the paraffin tape transfer system on MMA blocks. One needs to remove the tape with an aliphatic or aromatic hydrocarbon before hydration and subsequent staining. Phillip was describing tape transfer method for cryosections. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From rjr6 <@t> psu.edu Wed Jul 21 12:39:10 2004 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Agarose blocks Message-ID: <00a801c46f49$a1dd7f00$8861ba92@padlspsu.psu.edu> I just received tissue in agarose and I tried cutting a couple of them with very little luck. I have never worked with this before. Is there a trick to sectioning it? Should it have been processed differently? I didn't know what was in the block until I embedded it. Thank you for any help. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University From japoteete <@t> saintfrancis.com Wed Jul 21 12:37:12 2004 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Disposing of expired IHC reagents: Survey Message-ID: Our hospital Safety Department prefers that we use the biohazard waste system for antibody and detection system reagent disposal. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com > -----Original Message----- > From: Joe Nocito [SMTP:JNocito@Pathreflab.com] > Sent: Wednesday, July 21, 2004 11:49 AM > To: Histonet > Subject: [Histonet] Disposing of expired IHC reagents: Survey > > Hey 'netters, > Need a little info if you please. How are you disposing of expired primary > antibodies and detection reagents? In the trash? Biohazard? Drain? Big > bonfire? > > > Joe Nocito, BS, HT(ASCP) QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From subratab <@t> bdonline.com Wed Jul 21 12:47:41 2004 From: subratab <@t> bdonline.com (Subratab) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] formalin nomenclature Message-ID: <200407211749.i6LHnaCa027768@mailout.proshikanet.com> Dear all Here is a nice letter regarding the nomenclature of formalin: Manoonkitiwongsa PS, Schultz RL. Proper nomenclature of formaldehyde and paraformaldehyde fixatives for histochemistry. Histochem J. 2002 Jun-Jul;34(6-7):365-7. This paper is nice-reading and its helpfull to avoid discrepancy in describing fixatives in literature. Subrata Biswas, MD Dept of Nephrology University of Campinas SP, Brazil. From Evelyn.Flynn <@t> childrens.harvard.edu Wed Jul 21 12:58:11 2004 From: Evelyn.Flynn <@t> childrens.harvard.edu (Flynn, Evelyn) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Blood vessel antibodies for rabbit models Message-ID: Dear Zarana, Some years ago I purchased a polyclonal goat anti-human Factor VIII (von Willebrand) which cross-reacted with rabbit, from Incstar Corporation. The staining with FFPE sections was very satisfactory. However, I have been unable to purchase more from this company, and I would love to find a new supplier. Regards, Evelyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of degaboh@rice.edu Sent: Tue 7/20/2004 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blood vessel antibodies for rabbit models Hello all, I'd like to use rack the brains of all you histonetters. Can anyone recommend antibodies to CD31 and/or CD34 that can be used in rabbit models? Or, can anyone recommend other antibodies for blood vessels that can be used in rabbit models? I'm having a hard time finding these. Thanks! Zarana Patel degaboh@rice.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Wed Jul 21 13:24:20 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Oil of wintergreen Message-ID: The best thing for odors is X-O odor neutralizer. http://www.xocorp.com/ Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> 7/21/2004 7:04:55 AM >>> Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Wed Jul 21 13:38:19 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] AURANTIA Message-ID: Aurantia is a dye. Color Index number 10360. Try looking for it's synonym: Imperial Yellow. A German catalog identifies it as CI 18110 (benzol fast red B, FIAT 764). The free amine 2,2' ,4,4' ,6,6'-hexanitrodiphenylamine appears in chemical catalogs in reagent grade. From Conn's Biological Stains. I have several old vials of the dye. I can send you one if you can't find it. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> Jose Luis Palazon Fernandez 7/21/2004 4:59:58 AM >>> Dear Histonet fellows Do any of you know the comertial name of "Aurantia"? I need this reagent but I can?t find it in the chemical product catalogs. thanks in advance Jos? Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Jul 21 13:44:00 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] cryostat sectioning of unfixed human brains Message-ID: <002201c46f52$b07f2320$d1039a51@home> Be most grateful for Standard operating procedures/protocols/ info to set up system for the freezing of unfixed human brain material and cutting of cryostat sections therefrom. The tissue is not known to be infected but I appreciate that such tissue has to be treated as potentially infected. Thank you --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 From cwscouten <@t> myneurolab.com Wed Jul 21 13:47:22 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Agarose blocks Message-ID: What kind of microtome are you using? Soft things need to be cut with a Vibratome. How thin does it need to be? Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Wednesday, July 21, 2004 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Agarose blocks I just received tissue in agarose and I tried cutting a couple of them with very little luck. I have never worked with this before. Is there a trick to sectioning it? Should it have been processed differently? I didn't know what was in the block until I embedded it. Thank you for any help. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Wed Jul 21 13:51:47 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] DECONTAMINATING CRYOSTATS Message-ID: I do not thing such a thing is even theoretically possible. Kill TB germs buried in ice? Get a self decontaminating cryostat, and do the job overnight while you sleep. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Winters, Bert Sent: Wednesday, July 21, 2004 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DECONTAMINATING CRYOSTATS Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dencrowl <@t> MIT.EDU Wed Jul 21 14:02:28 2004 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Webmaster attention Message-ID: I was trying to log in with password "digest" to change to a digest format and this error message came up. thanks, Denise Bug in Mailman version 2.1.3 We're sorry, we hit a bug! If you would like to help us identify the problem, please email a copy of this page to the webmaster for this site with a description of what happened. Thanks! Traceback: Traceback (most recent call last): File "/home/mailman/scripts/driver", line 87, in run_main main() File "/home/mailman/Mailman/Cgi/options.py", line 206, in main password, user): File "/home/mailman/Mailman/SecurityManager.py", line 219, in WebAuthenticate print self.MakeCookie(ac, user) File "/home/mailman/Mailman/SecurityManager.py", line 228, in MakeCookie raise ValueError ValueError Python information: Variable Value sys.version 2.2.2 (#1, Feb 24 2003, 19:13:11) [GCC 3.2.2 20030222 (Red Hat Linux 3.2.2-4)] sys.executable /usr/bin/python sys.prefix /usr sys.exec_prefix /usr sys.path /usr sys.platform linux2 Environment variables: Variable Value CONTENT_LENGTH 53 CONTENT_TYPE application/x-www-form-urlencoded HTTP_REFERER http://lists.utsouthwestern.edu/mailman/options/histonet/dencrowl%40mit.edu SCRIPT_FILENAME /home/mailman/cgi-bin/options PYTHONPATH /home/mailman SERVER_SOFTWARE Apache/2.0.40 (Red Hat Linux) SERVER_ADMIN root@localhost SCRIPT_NAME /mailman/options SERVER_SIGNATURE Apache/2.0.40 Server at lists.utsouthwestern.edu Port 80 REQUEST_METHOD POST HTTP_HOST lists.utsouthwestern.edu PATH_INFO /histonet SERVER_PROTOCOL HTTP/1.1 QUERY_STRING REQUEST_URI /mailman/options/histonet HTTP_ACCEPT */* PATH_TRANSLATED /var/www/html/histonet HTTP_USER_AGENT Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en-us) AppleWebKit/85.7 (KHTML, like Gecko) Safari/85.7 HTTP_CONNECTION close SERVER_NAME lists.utsouthwestern.edu REMOTE_ADDR 18.114.1.35 REMOTE_PORT 59068 HTTP_ACCEPT_LANGUAGE en-us, ja;q=0.21, de-de;q=0.86, de;q=0.79, fr-fr;q=0.71, fr;q=0.64, nl-nl;q=0.57, nl;q=0.50, it-it;q=0.43, it;q=0.36, ja-jp;q=0.29, en;q=0.93, es-es;q=0.14, es;q=0.07 UNIQUE_ID Aj1mO8elmKIAADLdXUkAAAAI SERVER_PORT 80 GATEWAY_INTERFACE CGI/1.1 SERVER_ADDR 199.165.152.162 DOCUMENT_ROOT /var/www/html From cwscouten <@t> myneurolab.com Wed Jul 21 14:04:32 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] DECONTAMINATING CRYOSTATS Message-ID: I forgot to mention, full decontamination can be done in 3.5 hours, frozen to frozen, with thaw and decontamination steps in between. And no user interaction. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Winters, Bert Sent: Wednesday, July 21, 2004 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DECONTAMINATING CRYOSTATS Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Wed Jul 21 14:26:25 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] (Histonet) Sakura Auto Stainer Message-ID: Why not look for a stainer with 44 baths. myNeurolab.com offers the most versatile Tissue stainer in TST 44. it comes with 44 baths and will allow you to do up to 400 slides per minute. You can obtain more information about this product at the followign link. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=545201&catdesc=Histology+Equipment&CatThreeID=648&CatOneID=4&subcatdesc=Tissue+Staining&idsubcategory=192 If you have any question or would like a demonstration please contact us. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Wilson Sent: Friday, July 16, 2004 11:48 PM To: histonet@pathology.swmed.edu Subject: [Histonet] (Histonet) Sakura Auto Stainer I am currently considering purchasing a Sakura Automatic slide stainer- DRS 6000 (i think). I would like to set this instrument up to stain both Histology and Cytology slides. I understand the unit has only 27 resevoirs for reagents, but can any of the resevoirs be commonly used by both procedures? Or, to get to the point- does anyone do this with their stainer and would they be willing to share the procedure? Thanks Jerry Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From james.zimmerman <@t> pharma.novartis.com Wed Jul 21 14:38:00 2004 From: james.zimmerman <@t> pharma.novartis.com (james.zimmerman@pharma.novartis.com) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] C4D Message-ID: Hello, Does anyone have any experience with Compliment 4 D? If so, I would appreciate a vendor and protocol in monkey and/or mice. Thanks, JPZ From jmitchell <@t> neurology.wisc.edu Wed Jul 21 15:42:29 2004 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean)) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Darkroom Processor Message-ID: Is anyone familiar with or currently using a MohrPro 8 Darkroom Processor? I am looking for some feedback on this instrument before making the purchase. Thanks, Jean Mitchell University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI From SJones <@t> cvm.tamu.edu Wed Jul 21 15:06:09 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] hard paraffin Message-ID: What is the hardest paraffin on the market? I've found Gold Standard Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have any comments about the Gold Standard paraffins? Years ago, a lab used some sort of additive to their paraffin for bone that made the block yellow in color. Does anyone know what that could have been. I believe it was something like picrotin? Ring any bells? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From brett_connolly <@t> merck.com Wed Jul 21 16:00:33 2004 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] brightfield 3D reconstruction Message-ID: Aside from 3D reconstruction of confocal, fluorescent images, is anyone aware of any software/hardware for doing 3D reconstruction on serial sections of brightfield images? I have heard of VoxBlast from Vaytek, but that's about it. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From gcallis <@t> montana.edu Wed Jul 21 16:11:05 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] hard paraffin additive In-Reply-To: References: Message-ID: <6.0.0.22.0.20040721150518.01b1fa18@gemini.msu.montana.edu> Tissue Prep 2 is hard but its melting point is not that high, and check with Richard Allan - I think they have one also. I think you were thinking of piccolyte, a resin that dissolves in the melted paraffin. We add this to paraffin milleniums ago, before paraffin technology and additives improved. It made the paraffin supersticky plus it was a total pain to deal with, a huge mess. I recall the company that made it was called Hercules Corp, I am not sure you can even get it anymore. It come in huge bags and big chunks in the bag. There is a publication on this resin in J of Histotechnology back a few years. Personally, you should try to find one ready to go to save time. Surgipath has a blue ribbon paraffin, check with them also. At 02:06 PM 7/21/2004, you wrote: >What is the hardest paraffin on the market? I've found Gold Standard >Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have >any comments about the Gold Standard paraffins? Years ago, a lab used >some sort of additive to their paraffin for bone that made the block >yellow in color. Does anyone know what that could have been. I believe >it was something like picrotin? Ring any bells? Thanks, Sarah > >Sarah Jones HT(ASCP) >Dept. of Vet. Anatomy & Public Health >Histology Lab >Texas A&M University >College Station, TX 77843-4458 >phone: 979-845-3177 >fax: 979-458-3499 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Jul 21 16:17:58 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] brightfield 3D reconstruction In-Reply-To: References: Message-ID: <6.0.0.22.0.20040721151203.01b2ef90@gemini.msu.montana.edu> The publication, Microscopy Today frequently has lpublications on this technology. You can check out their website and do a search. Also, people who do confocal and associated with core imaging facilities probably do 3D reconstruction are easily accessed via confocal listserver out of Buffalo, ask the pertinent question. (you may already participate?) At 03:00 PM 7/21/2004, you wrote: >Aside from 3D reconstruction of confocal, fluorescent images, is anyone >aware of any software/hardware for doing 3D reconstruction on serial >sections of brightfield images? I have heard of VoxBlast from Vaytek, but >that's about it. > >Thanks, Brett Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From rocan <@t> mac.com Wed Jul 21 16:30:46 2004 From: rocan <@t> mac.com (Rocan) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Blood vessel antibodies for rabbit models In-Reply-To: References: Message-ID: <3A27E75C-DB5D-11D8-8142-000A9589219E@mac.com> I think there is a goat polyclonal against human vwf is now available through DAKO. Indeed this antibody stains vessels from human and mouse and I would expect it to cross also with rabbit. ----- Dr.Rocio Sierra-Honigmann Director Engineered Wound Repair Laboratory Cedars Sinai Research Institute Davis 1091 310-423-1882 Honigmannr@cshs.org On Jul 21, 2004, at 10:58 AM, Flynn, Evelyn wrote: > Dear Zarana, > Some years ago I purchased a polyclonal goat anti-human Factor > VIII (von Willebrand) > which cross-reacted with rabbit, from Incstar Corporation. The > staining with FFPE > sections was very satisfactory. However, I have been unable to > purchase more from > this company, and I would love to find a new supplier. > > Regards, > Evelyn > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > degaboh@rice.edu > Sent: Tue 7/20/2004 5:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Blood vessel antibodies for rabbit models > > Hello all, > > I'd like to use rack the brains of all you histonetters. Can anyone > recommend antibodies to CD31 and/or CD34 that can be used in rabbit > models? > Or, can anyone recommend other antibodies for blood vessels that can > be used > in rabbit models? I'm having a hard time finding these. > > Thanks! > > Zarana Patel > degaboh@rice.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Juanita.X.Brody <@t> kp.org Wed Jul 21 16:57:04 2004 From: Juanita.X.Brody <@t> kp.org (Brody,Juanita X) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Indirect Immunofluorescence Message-ID: <8220AFE580A4A34582D63C3D3B2C00EB0F60EF@cscrdemsg004.crdc.kp.org> We do indirect IF on skin specimens and having problems with false positives and background staining. Is there any one doing this procedure who would be willing to share the source of money slides, control sera, and procedure? Thanks in advance. From lpwenk <@t> sbcglobal.net Wed Jul 21 19:01:11 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] DECONTAMINATING CRYOSTATS References: <270614B321ACB44D8C1D91F4F921FDC3627774@NCH01EX02.nch.org> Message-ID: <008a01c46f7f$00df9940$a623d445@domainnotset.invalid> How about not allowing frozen sections on lung for the diagnosis of infectious diseases? TB, Pneumocystis, etc. Do a touch prep for the fresh tissue, and stain the slide that way. Make several slides, so can do H&E, Giemsa, GMS, Kinyoun, PASH, whatever is needed. Fix and process the tissue used for the touch prep, for diagnosis on a permanent section the next day. Most of the time, the touch prep will reveal the micro-organisms. Once in a great while, there is a false negative, which is picked up the next day on the fixed processed block. If it is explained to the surgeons that doing frozen sections on infectious lung tissue puts the cryostat out of commission for the rest of the day, and that no other surgeries, including all tumor cases, will have any FS done for the rest of that day, they are usually willing to accept the results of the touch preps. Occasionally, an infectious lung will still slip through, but usually because they thought the x-ray showed a tumor not an infection, or there is infection with the tumor. But there is no need to do a FS on an infectious case. Like you said, it decommissions the cryostat, to say nothing of the potential biohazardous risk the person doing the sectioning is in, if they accidentally cut themselves. You will need the support/backing of the pathologists. But they should support you, knowing that the cryostat won't be available for the rest of the day. (Even if they aren't worried about the risk to the sectioner.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Winters, Bert" To: Sent: Wednesday, July 21, 2004 11:52 AM Subject: [Histonet] DECONTAMINATING CRYOSTATS Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From swaram <@t> myrealbox.com Wed Jul 21 20:02:15 2004 From: swaram <@t> myrealbox.com (Swaram) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Pan Melanoma cocktail from "Not" Mouse...?? In-Reply-To: <001f01c46908$ce4ca1c0$0a01a8c0@LUCYSALES> References: <40F42807.3040903@myrealbox.com> <001f01c46908$ce4ca1c0$0a01a8c0@LUCYSALES> Message-ID: <40FF1217.5090905@myrealbox.com> Dear Histonetters, I am in need of Pan Melanoma cocktail from any species other than Mouse. I have already tried one other Ab (polyclonal Rabbit Mart-1/Melan A) and it did not do a good job compared to the Pan Melanoma cocktail (containing HMB-45, MART-1 & Tyrosinase). The Ab should is to be used for IF and on paraffin fixed tissue. Anybody who makes them, kindly contact me. Any information from histonetters would be appreciated. Thanks, Swaram. From SJones <@t> cvm.tamu.edu Wed Jul 21 20:57:14 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] hard paraffin additive Message-ID: Thanks for your reply Gayle. From the info you gave, I found piccolyte on the Hercules web site. Still looking for the J of H article. Wouldn't paraffin hardness be a factor of melting point, with the higher melting point being the harder paraffin? Thanks, Sarah >>> Gayle Callis 7/21/2004 4:11:05 PM >>> Tissue Prep 2 is hard but its melting point is not that high, and check with Richard Allan - I think they have one also. I think you were thinking of piccolyte, a resin that dissolves in the melted paraffin. We add this to paraffin milleniums ago, before paraffin technology and additives improved. It made the paraffin supersticky plus it was a total pain to deal with, a huge mess. I recall the company that made it was called Hercules Corp, I am not sure you can even get it anymore. It come in huge bags and big chunks in the bag. There is a publication on this resin in J of Histotechnology back a few years. Personally, you should try to find one ready to go to save time. Surgipath has a blue ribbon paraffin, check with them also. At 02:06 PM 7/21/2004, you wrote: >What is the hardest paraffin on the market? I've found Gold Standard >Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have >any comments about the Gold Standard paraffins? Years ago, a lab used >some sort of additive to their paraffin for bone that made the block >yellow in color. Does anyone know what that could have been. I believe >it was something like picrotin? Ring any bells? Thanks, Sarah > >Sarah Jones HT(ASCP) >Dept. of Vet. Anatomy & Public Health >Histology Lab >Texas A&M University >College Station, TX 77843-4458 >phone: 979-845-3177 >fax: 979-458-3499 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lawrence.Brett <@t> luht.scot.nhs.uk Thu Jul 22 02:54:02 2004 From: Lawrence.Brett <@t> luht.scot.nhs.uk (Brett, Lawrence) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Galectin-3 Message-ID: <857344E7D8F90240935A288998A89D811E774B@wgh-ex1.luht.scot.nhs.uk> Yes we use GAL 3 for Thyroid tumours too, along with Cytokeratin 19. Papillary Ca's are positive with CK19 and mostly negative with Gal 3 while follicular ca's are mostly positive with GAL 3 and mostly negative with CK19. See Beesley & McLaren. Histopathology 41. 3. p236 (2002). Lawrence Brett Royal Infirmary Edinburgh Scotland -----Original Message----- From: Sawrenko, Christina [mailto:csawrenk@bccancer.bc.ca] Sent: 21 July 2004 22:07 To: Brett, Lawrence Subject: RE: [Histonet] Galectin-3 Thanks for the info. For what application do you use the antibody? We are interested in using it for thyroid neoplasms. Thanks again, Chris -----Original Message----- From: Brett, Lawrence [ mailto:Lawrence.Brett@luht.scot.nhs.uk ] Sent: Wednesday, July 21, 2004 8:11 AM To: 'Sawrenko, Christina' Subject: RE: [Histonet] Galectin-3 We use Novocastra's Galectin 3 but not with CD44v6. Gal 3 works well with Tris-EDTA heat antigen retrieval. We use a microwave pressure cooker (Biogenex) and incubate for 30 mins in primary diluted at 1:150. Hope this is of some value. Lawrence Brett Immunocytochemistry Lab. Royal Infirmary Edinburgh Scotland -----Original Message----- From: Sawrenko, Christina [ mailto:csawrenk@bccancer.bc.ca ] Sent: 16 July 2004 18:21 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Galectin-3 Good morning, One of our pathologists has asked us to research Galectin-3 for use in thyroid neoplasms. Does anyone have experience with this antibody and do you use it in conjuction with CD44v6 (Novocastra)? Thanks in advance for your help! Chris Sawrenko Histopathology BC Cancer Agency Vancouver BC Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** The information contained in this message may be confidential or legally privileged and is intended for the addressee only, If you have received this message in error or there are any problems please notify the originator immediately. The unauthorised use, disclosure, copying or alteration of this message is strictly forbidden. ********************************************************************** ********************************************************************** The information contained in this message may be confidential or legally privileged and is intended for the addressee only, If you have received this message in error or there are any problems please notify the originator immediately. The unauthorised use, disclosure, copying or alteration of this message is strictly forbidden. ********************************************************************** From mab70 <@t> medschl.cam.ac.uk Thu Jul 22 03:46:53 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Agarose blocks Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138C@mius2.medlan.cam.ac.uk> I have cut lots of agar blocks and used the normal tissue processing schedules of whichever lab I worked in. I usually post fixed the agar block in Neutral Buffered Formalin for a few hours to a day after embedding depending upon how well fixed the sample was prior to embedding in the agar and processing. I do think your client ought to have told you what was embedded in the agarose else how do you know how much to trim or what the orientation is? I have used this procedure to orientate isolated hair follicles and flat intestine samples so that I could get sections of precise areas, so it was essential to have all the available information about the sample. In difficult cases, I hand processed the agar blocks to paraffin, but this should not be necessary if you have a process that is suited to the tissue sample itself. I always used 2% w/v aqueous agar (Difco) and maintained it at 55 to 60C in a waterbath to keep it molten. I made a mould suited to the sample, e.g. a cut off syringe, pipetted a layer of agar into it and allowed it to start gelling, I then layered on my sample so as to orientate it correctly, then pipetted a few more drops of agar on top, taking care to allow it to cool sufficiently to avoid "cooking" my sample. To achieve the latter, I drew up some agar into a pastette and held it at room temperature for a few seconds to allow it to cool, trial and error taught me how long to do this. I have to say that I have used agar for both paraffin and frozen sectioning successfully. My hand process went through 70% ethanol, 3 changes of absolute ethanol and 1 of histoclear each step being 1 hour, then overnight in histoclear, followed by a further histoclear of 1 hour, 2 changes of wax in the wax oven for 1 hour then embed. This worked well for me. I hope this helps. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Roberta Horner [mailto:rjr6@psu.edu] Sent: Wednesday, July 21, 2004 6:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Agarose blocks I just received tissue in agarose and I tried cutting a couple of them with very little luck. I have never worked with this before. Is there a trick to sectioning it? Should it have been processed differently? I didn't know what was in the block until I embedded it. Thank you for any help. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Thu Jul 22 03:49:50 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] brightfield 3D reconstruction Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138D@mius2.medlan.cam.ac.uk> I'm not sure if I'm right but I think that Olympus' software, AnalySIS can do this, why not contact your Olympus rep? Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Connolly, Brett M [mailto:brett_connolly@merck.com] Sent: Wednesday, July 21, 2004 10:01 PM To: 'HISTONET' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] brightfield 3D reconstruction Aside from 3D reconstruction of confocal, fluorescent images, is anyone aware of any software/hardware for doing 3D reconstruction on serial sections of brightfield images? I have heard of VoxBlast from Vaytek, but that's about it. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ---------------------------------------------------------------------------- -- Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ---------------------------------------------------------------------------- -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marion.Hiles <@t> north-bristol.swest.nhs.uk Thu Jul 22 04:06:01 2004 From: Marion.Hiles <@t> north-bristol.swest.nhs.uk (Marion Hiles) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Abs to mycobacterium tuberculosis Message-ID: <2EE924DF60902943AC6E2EF35155451F19CD29@nbfexch03.north-bristol.nhs> Does anyone out there use immuno for staining acid fast bacilli ( mycobacterium tuberculosis) on FFPE sections? If so, how good is it? Novocastra supply one, so I will be making enquiries with them. Bob Quilty Dept. Neuropathology Frenchay Hospital BRISTOL UK DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorised. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. From tflore <@t> lsuhsc.edu Thu Jul 22 10:04:20 2004 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Indirect Immunofluorescence Message-ID: Juanita, Our EM Lab performs immunofluorescent studies on renal, oral and skin biopsies. We supply our "customers" with Michel's Transport Media (sold commercially as Zeus) We use "charged" slides to pick up all our frozen sections (sectioned at 6 microns). We fix the frozen oral, skin or renal sections in EM Grade cold Acetone for 30 minutes, PBS for 30 min and then incubate with titrated antibodies (according to manufactured are titrated): IgG, IgM, IgA, C3, C1q, C4d(C4d is time consuming, costly and requires 4 steps=2hrs), CMV, Polymiovirus (requires 2 steps = 2 hrs), Kappa, Lambda, and Fibrinogen for 1 hour. PBS 2x washes Coverslip with BioMedia Hold in refrigerator without a bulb and no fixative inside the refrigerator until pathologist is ready to view. NOTE: no immunofluorescent light is allowed on sections as they tend to fade. Our products are most but not exclusively from DAKO. Teresa Flores LSUHSC New Orleans, LA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brody,Juanita X Sent: Wednesday, July 21, 2004 4:57 PM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] Indirect Immunofluorescence We do indirect IF on skin specimens and having problems with false positives and background staining. Is there any one doing this procedure who would be willing to share the source of money slides, control sera, and procedure? Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jul 22 10:10:33 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] More on hard paraffin additive - long discussion In-Reply-To: References: Message-ID: <6.0.0.22.0.20040722083124.01ae4008@gemini.msu.montana.edu> Hi Sarah, Possibly could be the answer. But your question "Wouldn't paraffin hardness be a factor of melting point, with the higher melting point being the harder paraffin?" doesn't seem the case with harder Tissue Prep 2. m.p. 55C - 57C! Every manufacturer has different additives, and depending on what they use, different resins, etc, melting points really vary all over the place. Consequently, I don't think higher mp necessarily means harder paraffin. We use Tissue Prep 2 bone here (can either embed after using your regular, favorite paraffin in processor for infiltration OR infiltrate and embed in the Tissue Prep 2 (the ideal situation for labs working with large volume of bone). We prefer to NOT have the infiltration temperature above 60C for bone, with longer processing - higher temp to maintain melted paraffin tends to really dry out and harden bone. This is probably a combination of type of clearant used, length of time in dehydrants, long exposures to heat of paraffin. When we infiltrate with Tissue Prep 2 , the temperature is set at 58C with alternating vacuum and pressure (VIP). We avoid mp above 60C just because these paraffins are generally infiltrated at that higher temperature. I personally do not like 62C for infiltration of bone. I will opt for lower temp (60C and lower) particularly with 3 - 4 changes of paraffin at 58C at 2 hours per change for larger bone samples. One paraffin combination used for years, before using Tissue Prep 2, was Surgipath's Infiltration medium (separate in processor) and then Embedding medium. I don't think it was necessarily a harder paraffin, but controlling decalcification and processing, we had superb bone sections and was excellent for 1 to 2 um kidney biopsy sections. We have found we could use just about any paraffin for bone work IF all else is in place e.g. decalcification and processing issues. Harder paraffin is certainly a bonus, but not always an available choice if you do not routinely like it for soft tissues. At this point we infiltrate with Tissue Prep, and embed in Tissue Prep 2 which works fine for whole mouse heads, mouse paws, femurs, tibias, hamster upper nasal turbinates with partial skull, whole rat heads, femurs, tibias. If I find reference on piccolyte, I will let you know, it was a long way back, probably in 1980's. When we used this, we had a paraffin that looked like hockey pucks, from Scientific Products - whew, that over 30 years ago. The little hockey pucks didn't have much in the way of additive at that time. It was a tedious, timely, messy job to melt, stir piccolyte into paraffin, and then filter. If you can find a hard paraffin that works for you, buy it! From mab70 <@t> medschl.cam.ac.uk Thu Jul 22 10:21:16 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Microscope filters Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138E@mius2.medlan.cam.ac.uk> Hi all, I have a chinese colleague who requires a blue filter to suppress staining for Nissl substance (violet) in brightfield transmitted light microscopy - does anyone know what filter this is and a supplier of such a filter. So far I have only identified a Daylight filter, but I am not sure if this would do the trick or not. If anyone knows anything about this I would be very grateful as would my colleague. Thanks in anticipation. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR From gsmith <@t> confocal.com Thu Jul 22 10:28:32 2004 From: gsmith <@t> confocal.com (Glenn Smith) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] brightfield 3D reconstruction - COMMERCIAL Message-ID: <001d01c47000$912e2d50$1f05a8c0@confocal.com> Brett, We have recently released our TISSUEscope instrument for both fluorescence and brightfield imaging. Software is available with the system for the optical sectioning you refer to. [Among other places this fall, the instrument can be seen at the NSH event in Toronto in September - we'll be there in conjunction with Beecher Instruments]. However, while we have instruments in our lab setup for confocal detection in both transmission (brightfield) and fluorescence, the default setup for detection methods in the TISSUEscope is such that the brightfield images are acquired in a non-confocal way. I'd be interested in hearing back from you and others as to the background on this question, usefulness of capability etc. as we may want to revisit the default setup! You may also be interested in the first page of an early article on the subject of confocal transmission imaging that can be seen for free on Nature at http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v351/n6327/a bs/351551a0.html - you'll need a subscription to access the pdf for entire copy. We have hardcopy as well if you'd like a copy - pls contact me offline if that's the case. Regards Glenn Glenn Smith, P.Eng. 519.886.9013 x38 gsmith@confocal.com Biomedical Photometrics Inc/GeneFocus Widefield Confocal Scanning Instruments and Software ------------------------------ Message: 18 Date: Wed, 21 Jul 2004 17:00:33 -0400 From: "Connolly, Brett M" Subject: [Histonet] brightfield 3D reconstruction To: "'HISTONET' (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain Aside from 3D reconstruction of confocal, fluorescent images, is anyone aware of any software/hardware for doing 3D reconstruction on serial sections of brightfield images? I have heard of VoxBlast from Vaytek, but that's about it. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com From mcauliff <@t> umdnj.edu Thu Jul 22 13:44:12 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Microscope filters In-Reply-To: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138E@mius2.medlan.cam.ac.uk> References: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138E@mius2.medlan.cam.ac.uk> Message-ID: <41000AFC.7050708@umdnj.edu> Hi Margaret: Assuming your colleague is using black and white negative film, a blue filter will pass more blue light to the film and give more density in the negative. When printed, that will translate to the blue subject being lighter on the print. That said, there are lots of blue filters used in photography and some trial and error may be needed. There are lots of different "daylight filters", depending on the light source and the amount of correction needed, an 80A filter might be blue enough for your application. The standard blue filter for making RGB separations is a #47 and it is a fairly dense blue-purple color. A #46 is very similar. Such filters are sold in photo stores that cater to professional photographers (Jessop's in the UK is one) but you might be able to find something in the AudioVisual dept at your institution or perhaps the manufacturer of your microscope might loan you several for a trial, you could then purchase the one that fits your needs. Geoff Margaret Blount wrote: >Hi all, > >I have a chinese colleague who requires a blue filter to suppress staining >for Nissl substance (violet) in brightfield transmitted light microscopy - >does anyone know what filter this is and a supplier of such a filter. So far >I have only identified a Daylight filter, but I am not sure if this would do >the trick or not. If anyone knows anything about this I would be very >grateful as would my colleague. > >Thanks in anticipation. > >Margaret > >Margaret Blount >Chief Technician >Clinical Biochemistry >University of Cambridge >Addenbrooke's Hospital >Hills Road >Cambridge >CB2 2QR > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jul 22 10:59:57 2004 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] water bath adhesives Message-ID: I am looking into possibly changing the waterbath adhesive and would like to get some input from others as to which products you prefer?? Also, aren't there some of the adhesives on the market that can interfere with certain IHC staining? Thanks ahead of time for all of the help!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From ftulenko06 <@t> jcu.edu Thu Jul 22 11:04:20 2004 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] museum specimens Message-ID: I will be sectioning museum specimens of turtle embryos that were initially fixed with buffered formalin and then preserved in 70% Ethanol. They have been in alcohol for the last 2-3 years. Does anybody know a good protocol for processing museum specimens? Do they have to be refixed? How well will they take up stain after being in alcohol so long? Thanks for any advice. Frank From Debbie.Vigil <@t> AHSS.org Thu Jul 22 11:23:35 2004 From: Debbie.Vigil <@t> AHSS.org (Vigil, Debbie (CTMC / CTPA)) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] HT certification practice test Message-ID: <04Jul22.123527edt.119091@gateway.ahss.org> I have three OJT HT's that will be taking their exam very soon. Does anyone have any practice tests they are willing to share? I am aware of an online test. However, I was unable to locate the website. Any help would be appreciated. Thanks in Advance CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From chris <@t> ptplab.com Thu Jul 22 11:27:59 2004 From: chris <@t> ptplab.com (Christopher L. Robertson) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] Histology Software Message-ID: Hi everyone. I am new to Histonet and was told this is the best source of information when it comes to histology. I work for a small, independent histology laboratory, and we are looking for software that relates to histology/pathology. Any recommendations? Resources? Thank you. CR From scoop <@t> mail.nih.gov Thu Jul 22 11:29:14 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] formalin nomenclature Message-ID: Thanks to everyone for the info on formalin nomenclature! Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From Charlene.Henry <@t> STJUDE.ORG Thu Jul 22 11:31:15 2004 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Sep 16 15:23:46 2005 Subject: [Histonet] (no subject) Message-ID: <5CB39BCA5724F349BCB748675C6CA1A202C75452@SJMEMXMB02.stjude.sjcrh.local> Please tell how to unsubscribe. I'm going on vacation and will be turning on my "Out of Office" message. I have tried to unsubscribe to no avail. Charlene From Janet.Bonner <@t> FLHOSP.ORG Thu Jul 22 12:53:25 2004 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] water bath adhesives Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3FEF@fh2k093.fhmis.net> We use Sta-on, a Surgipath product, in our waterbathes when using regular slides. We do not use any adhesive in our waterbath (just plain water) when using the "Plus" slides - we find this extremely useful for IHC. -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: Thursday, July 22, 2004 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath adhesives I am looking into possibly changing the waterbath adhesive and would like to get some input from others as to which products you prefer?? Also, aren't there some of the adhesives on the market that can interfere with certain IHC staining? Thanks ahead of time for all of the help!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From juan.gutierrez <@t> christushealth.org Thu Jul 22 13:09:32 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] water bath adhesives Message-ID: We use to do the same thing, but it got confusing as to which waterbath to use or which slide to use. Now we only use plus coated slides for everything and no additives in the water. The cost difference is not that significant to continue using both kinds of slides. On the other hand, beware of using just one supplier of slides. They like to get complacent and their QC goes out the drain as well as your sections. I always rotate ordering from three different vendors. It might sound paranoid, but I've been burned to many times before. IT DOESN'T MATTER WHO MANUFACTURES THEM, THEY ALL FAIL OCCASIONALLY. No vendors need to reply. Hope this helps. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG] Sent: Thursday, July 22, 2004 12:53 PM To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] water bath adhesives We use Sta-on, a Surgipath product, in our waterbathes when using regular slides. We do not use any adhesive in our waterbath (just plain water) when using the "Plus" slides - we find this extremely useful for IHC. -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: Thursday, July 22, 2004 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath adhesives I am looking into possibly changing the waterbath adhesive and would like to get some input from others as to which products you prefer?? Also, aren't there some of the adhesives on the market that can interfere with certain IHC staining? Thanks ahead of time for all of the help!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Thu Jul 22 13:25:53 2004 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Re:How to unsubscribe from Histonet etc. Message-ID: Dear Charlene (and other Histonetters); To unsubscribe from the list you need to go to the Histonet server internet page http://lists.utsouthwestern.edu/mailman/listinfo/histonet (this address is at the bottom of all Histonet messages). At the bottom of this server page is an area for Histonet subscribers. Enter your email address in the field at the bottom and select "unsubscribe or edit.." (Don;t use the area for administrators). The next field will ask you for your password. Once your password is entered you can edit your address, switch to digest mode, unsubscribe etc. If you don't remember your password you can request it from this site also. Let me know if you have problems. Linda M Histonet administrator >>> "Henry, Charlene" 7/22/2004 11:31:15 AM >>> Please tell how to unsubscribe. I'm going on vacation and will be turning on my "Out of Office" message. I have tried to unsubscribe to no avail. Charlene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Jul 22 13:40:07 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Vigil,debbie/HT questions Message-ID: <000501c4701b$4ff990c0$1d2a14ac@wchsys.org> American Society of Clinical Pathologists have "A Self-Assessment Workbook" is available from ASCP Press. You should be able to get information from the ASCP website. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Sue.Kapoor <@t> uhsi.org Thu Jul 22 13:59:06 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] cryostat oil Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5620@khmcexch.uhsi.org> Can anyone tell where I can order "Reichert-Jung Cryostat Microtome Lubricant"? the empty bottle I have has a catalog #970 if that helps. many thanks in advance!! Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From TJJ <@t> Stowers-Institute.org Thu Jul 22 14:02:44 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Museum specimens Message-ID: Frank, I have never worked on museum specimens, but I have taken a workshop from someone who has. Helen Wimer at the Smithsonian Institution. Interesting workshop. I will send you her email address under separate email. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From mari.ann.mailhiot <@t> leica-microsystems.com Thu Jul 22 14:17:35 2004 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] cryostat oil Message-ID: Sue You can order your cryostat oil from Leica. Give me a call if you need a part number. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Kapoor, Sue" To: histonet@lists.utsouthwestern.edu Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] cryostat oil western.edu 07/22/2004 01:59 PM Can anyone tell where I can order "Reichert-Jung Cryostat Microtome Lubricant"? the empty bottle I have has a catalog #970 if that helps. many thanks in advance!! Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From histokiwi <@t> yahoo.com Thu Jul 22 15:26:26 2004 From: histokiwi <@t> yahoo.com (Janice McDonald) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Repetitive motion Syndrome in particular use of shoulders. Message-ID: <20040722202626.70565.qmail@web20805.mail.yahoo.com> I sustained an injury to my left shoulder while at work in the lab when I fell off a defective stool and landed on the floor hitting my shoulder. The insurance company is refusing to pay for my treatment as their Occupational Specialist has stated that histology work is not repetitive and in particular the shoulder is not affected.They have requested that I provide information to the contrary if I wish to have my case reviewed.We were using an older model Microm where the left handle came 4/5ths of the way to the top of the microtome which required my arm to be in a slightly raised position causing me considerable discomfort.The lab is a research lab at a medical school and all our staining and coverslipping were done manually in a large fume hood that had a lowered glass door. I am hoping that some-one knows or has on file any documented information that they can send to me relating to RMS in particular mentioning the shoulder that I can present it to the insurer. Thanks ! Jan McDonald histokiwi@yahoo.com Skin Institute Lake Rd, Takapuna Auckland New Zealand. __________________________________ Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! http://promotions.yahoo.com/new_mail From Jackie.O'Connor <@t> abbott.com Thu Jul 22 15:51:43 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Repetitive motion Syndrome in particular use of shoulders. Message-ID: My Dad was an expert in the field of personal injury, particulary workman's compensation. Sounds like your workplace is jerking you around. I know nothing about how the laws work down under, but if you were here, you should immediately contact a reputable personal injury attorney, (aka ambulance chaser), who specializes in these kinds of cases. Any good attorney will take your case for no money up front, but will charge you about 30% of whatever monies you recover. You were hurt at work, you cannot perform your work due to your injury - their fault, their liability. Not all lawyers are evil-doers. Good luck. Janice McDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 07/22/2004 03:26 PM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Repetitive motion Syndrome in particular use of shoulders. I sustained an injury to my left shoulder while at work in the lab when I fell off a defective stool and landed on the floor hitting my shoulder. The insurance company is refusing to pay for my treatment as their Occupational Specialist has stated that histology work is not repetitive and in particular the shoulder is not affected.They have requested that I provide information to the contrary if I wish to have my case reviewed.We were using an older model Microm where the left handle came 4/5ths of the way to the top of the microtome which required my arm to be in a slightly raised position causing me considerable discomfort.The lab is a research lab at a medical school and all our staining and coverslipping were done manually in a large fume hood that had a lowered glass door. I am hoping that some-one knows or has on file any documented information that they can send to me relating to RMS in particular mentioning the shoulder that I can present it to the insurer. Thanks ! Jan McDonald histokiwi@yahoo.com Skin Institute Lake Rd, Takapuna Auckland New Zealand. __________________________________ Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! http://promotions.yahoo.com/new_mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Thu Jul 22 16:00:13 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Repetitive motion Syndrome in particular use of sh oulders. Message-ID: I emailed Janice a 1997 article on Repetitive Motion Injuries from Advance for Medical Laboratory Professionals that specifically mentions histotechs. Gary Gill -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Thursday, July 22, 2004 3:52 PM To: Janice McDonald Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Repetitive motion Syndrome in particular use of shoulders. My Dad was an expert in the field of personal injury, particulary workman's compensation. Sounds like your workplace is jerking you around. I know nothing about how the laws work down under, but if you were here, you should immediately contact a reputable personal injury attorney, (aka ambulance chaser), who specializes in these kinds of cases. Any good attorney will take your case for no money up front, but will charge you about 30% of whatever monies you recover. You were hurt at work, you cannot perform your work due to your injury - their fault, their liability. Not all lawyers are evil-doers. Good luck. Janice McDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 07/22/2004 03:26 PM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Repetitive motion Syndrome in particular use of shoulders. I sustained an injury to my left shoulder while at work in the lab when I fell off a defective stool and landed on the floor hitting my shoulder. The insurance company is refusing to pay for my treatment as their Occupational Specialist has stated that histology work is not repetitive and in particular the shoulder is not affected.They have requested that I provide information to the contrary if I wish to have my case reviewed.We were using an older model Microm where the left handle came 4/5ths of the way to the top of the microtome which required my arm to be in a slightly raised position causing me considerable discomfort.The lab is a research lab at a medical school and all our staining and coverslipping were done manually in a large fume hood that had a lowered glass door. I am hoping that some-one knows or has on file any documented information that they can send to me relating to RMS in particular mentioning the shoulder that I can present it to the insurer. Thanks ! Jan McDonald histokiwi@yahoo.com Skin Institute Lake Rd, Takapuna Auckland New Zealand. __________________________________ Do you Yahoo!? Yahoo! Mail - 50x more storage than other providers! http://promotions.yahoo.com/new_mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From waltersk <@t> mail.medicine.uiowa.edu Thu Jul 22 16:49:57 2004 From: waltersk <@t> mail.medicine.uiowa.edu (Walters, Katherine S) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Looking for Hazel Horn Message-ID: Hazel, if you're out there, could you email me? Kathy Walters From chris <@t> ptplab.com Thu Jul 22 17:41:17 2004 From: chris <@t> ptplab.com (Christopher L. Robertson) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Histology Software Message-ID: Thank you for your replies. I guess I need to be even more specific. We are looking for a software that works with accessioning, grossing, dictation, specials...etc...is there anything out there? Thanks. CR From lpwenk <@t> sbcglobal.net Thu Jul 22 20:08:08 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] HT certification practice test References: <04Jul22.123527edt.119091@gateway.ahss.org> Message-ID: <003a01c47051$85119380$a23dd445@domainnotset.invalid> ASCP Board of Registry (BOR) webpage has a link to the ASCP BOR on-line practice exams. Go to: http://www.ascp.org/bor/ Scroll about 3/4 of the way down the page. They will need to purchase a 90 subscription for access to the site (take as many times as they like). It's $30. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Vigil, Debbie (CTMC / CTPA)" To: Sent: Thursday, July 22, 2004 12:23 PM Subject: [Histonet] HT certification practice test > I have three OJT HT's that will be taking their exam very soon. > Does anyone have any practice tests they are willing to share? > I am aware of an online test. However, I was unable to locate the website. > Any help would be appreciated. > > Thanks in Advance > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain information > that is privileged, confidential and exempt from disclosure under applicable > law. Any unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sebres <@t> comcast.net Thu Jul 22 18:43:08 2004 From: sebres <@t> comcast.net (sebres@comcast.net) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Can Weil's stain be used on fresh-frozen tissue? Message-ID: <072220042343.24880.4100510C00023F970000613022007481849C0A9D0D0A9C@comcast.net> I've found several protocols for "Weil's method" for myelin stain, but they all seem to assume formalin-fixed, paraffin-embedded tissue. Does anyone know whether it will work in fresh-frozen tissue, perhaps with some formalin fixation of the slide-mounted tissue first? Thanks for any advice! Susan Bachus From rockbeki <@t> ufl.edu Thu Jul 22 20:40:46 2004 From: rockbeki <@t> ufl.edu (SMITH,REBEKAH FELICIA) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] water bath adhesives Message-ID: <1095146345.1090546846305.JavaMail.osg@spnode15> I'm kinda paranoid about my sections falling of in IHC (antigen retrieval can cause them to fall off, and besides IHC takes so long that I don't want to have my sections fall off in my last water rinse and have wasted all that time) so I both add 1 tsp of gelatin to my water bath and coat my slides with poly-l-lysine. The other person who works in my lab has used gelatin in the water bath and then plus slides with good results as well. (I don't use plus slides mainly because they are more expensive.) Rebekah Smith On Thu Jul 22 13:53:25 EDT 2004, "Bonner, Janet" wrote: > We use Sta-on, a Surgipath product, in our waterbathes when using > regular > slides. We do not use any adhesive in our waterbath (just plain > water) when > using the "Plus" slides - we find this extremely useful for IHC. > > -----Original Message----- > From: Dorothy.L.Webb@HealthPartners.Com > [mailto:Dorothy.L.Webb@HealthPartners.Com] > Sent: Thursday, July 22, 2004 12:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] water bath adhesives > > > > I am looking into possibly changing the waterbath adhesive and > would like to > get some input from others as to which products you prefer?? > Also, aren't > there some of the adhesives on the market that can interfere with > certain > IHC staining? Thanks ahead of time for all of the help!! > ________________________________________ > > This e-mail and any files transmitted with it are confidential > and are intended solely for the use of the individual or entity > to whom they > are addressed. If you are not the intended recipient or the > individual > responsible for delivering the e-mail to the intended recipient, > please > be advised that you have received this e-mail in error and that > any > use, dissemination, forwarding, printing, or copying of this > e-mail > is strictly prohibited. > > If you have received this e-mail in error, please immediately > notify > the HealthPartners Support Center by telephone at (952) 967-6600. > You will be reimbursed for reasonable costs incurred in notifying > us. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be privileged > and/or > confidential and protected from disclosure. If the reader of > this message > is not the intended recipient or agent responsible for delivering > this > message to the intended recipient, you are hereby notified that > any > dissemination, distribution or copying of this communication is > strictly > prohibited. If you have received this communication in error, > please notify > the sender immediately by replying to this message and deleting > the material > from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- SMITH,REBEKAH FELICIA "You are a child of the universe, no less than the trees and the stars You have a right to be here and whether or not it is clear to you, no doubt the universe is unfolding as it should. Therefore be at peace with G-d, whatever you conceive Him to be. And whatever your labors and aspirations,in the noisy confusion of life, keep peace in your soul.-Max Ehrmann,"Desiderata" From Gervaip <@t> aol.com Thu Jul 22 20:49:56 2004 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Repetitive motion Syndrome in particular use of sh oulders. Message-ID: <158.3a726c5b.2e31c8c4@aol.com> Obviously the compensation person has never stepped a foot in a histology lab. Most of our duties require repetitive motion. Your injury is the outcome of a fall off a faulty chair. Because of your shoulder injury from the fall you are unable to perform certain duties. Shoulder injuries can be difficult to treat and with medical care and therapy it is a long journey. Your shoulder most likely will never be perfect again, but you can get it to a point where most of your range of motion can be returned to you. It is unimaginable how the compensation person can even make such an uneducated statement. But then again they have a lot of stupid people around. In order for your shoulder to get better, you do need to do your exercises and move that joint or face the possibility of forming adhesions and then the joint will be permanently frozen in place. . But microtomy because of the repetitive motion can cause even more inflammation.... Especially if you must be at this workstation for long periods. Having to sit at workstations (such as embedding) with your shoulders hunched up don't help either. Ask that a physical or occupational therapist come in and evaluate your work area. Don't give up. They are wrong and they are trying to get away with it. If they had spent a few bucks to buy a decent chair, your accident would not have happened. And they want to know why it is so difficult to find histologists! Good luck to you, Pearl From aldo.anile <@t> HDScientific.com.au Thu Jul 22 21:35:53 2004 From: aldo.anile <@t> HDScientific.com.au (Aldo Anile) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] RE: RE: (Histonet) Sakura Auto Stainer (Charles Scouten) Message-ID: The TST 44 by Medite has a large number reagent stations giving it the ability to stain large numbers of slides from different staining protocols in a short period of time. There are 44 stations in the Medite TST 44, 6 of these are water stations, along with 4 load stations, 4 unload stations and 30 staining stations. This number of stations allows for the staining of slides with H&E and PAP simultaneously. Depending upon your protocols, you will have reagent stations left over for other staining protocols. Eg. A standard H&E staining program may only require up to 13 stations. These would include two de-waxing, three re-hydrating, Haematoxylin, Eosin, blueing solution, three dehydrating and two clearing. A cytology PAP stain may only require up to 16 solutions. This allows for two initial dehydrating solutions, three stains, Haematoxylin, OG6 and EA50, a blueing solution, two 95% alcohol washes before the OG6 and two 95% alcohol washes between the OG6 and EA50 followed by four dehydrating alcohols and two clearing solutions. We have a number of users in your exact situation, please fell free to contact me to discuss this further. Aldo Anile Application Consultant HD Scientific Phone: + 61 3 9364 9569 Fax No: + 61 3 9364 9479 Mobile: 0408 471 485 E-mail: aldo.anile@hdscientific.com.au Web: www.hdscientific.com.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, 22 July 2004 18:53 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 8, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: ] Gold Chloride for Retic (Alton D. Floyd) 2. adhesive tape & MMA Sections - Turpentine? (Johnson, Teri) 3. Agarose blocks (Roberta Horner) 4. RE: Disposing of expired IHC reagents: Survey (Poteete, Jacquie A.) 5. formalin nomenclature (Subratab) 6. RE: Blood vessel antibodies for rabbit models (Flynn, Evelyn) 7. Re: Oil of wintergreen (Sarah Jones) 8. Re: AURANTIA (Sarah Jones) 9. cryostat sectioning of unfixed human brains (Carl) 10. RE: Agarose blocks (Charles Scouten) 11. RE: DECONTAMINATING CRYOSTATS (Charles Scouten) 12. Webmaster attention (Denise Crowley) 13. RE: DECONTAMINATING CRYOSTATS (Charles Scouten) 14. RE: (Histonet) Sakura Auto Stainer (Charles Scouten) 15. C4D (james.zimmerman@pharma.novartis.com) 16. Darkroom Processor (Mitchell (Jean)) 17. hard paraffin (Sarah Jones) 18. brightfield 3D reconstruction (Connolly, Brett M) 19. hard paraffin additive (Gayle Callis) 20. Re: brightfield 3D reconstruction (Gayle Callis) 21. Re: Blood vessel antibodies for rabbit models (Rocan) 22. Indirect Immunofluorescence (Brody,Juanita X) 23. Re: DECONTAMINATING CRYOSTATS (lpwenk@sbcglobal.net) 24. Pan Melanoma cocktail from "Not" Mouse...?? (Swaram) 25. Re: hard paraffin additive (Sarah Jones) 26. RE: Galectin-3 (Brett, Lawrence) 27. RE: Agarose blocks (Margaret Blount) 28. RE: brightfield 3D reconstruction (Margaret Blount) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Jul 2004 12:58:11 -0400 From: "Alton D. Floyd" Subject: Re: [Histonet] ] Gold Chloride for Retic To: David.Edmondson@christie-tr.nwest.nhs.uk Cc: Histonet@lists.utsouthwestern.edu Message-ID: <20040721.125814.-279241.1.al.floyd@juno.com> Content-Type: text/plain; charset=us-ascii Hello David, The procedure you are remembering is the generation of gold colloids for the purpose of performing immunostaining on plastic sections for electron microscopy. And you are correct, the color of the scattered light is indicative of the particle size. Not absolute, since individual eyes vary in color perception so much. Actual size generated depends on the conditions employed in manufacturing the good particles, which are then cleaned by ultracentrifugation prior to use. The colloid itself is generated by the protein coating (which can contain the primary antibodies) which is applied to the particles. Al Floyd 23126 South Shore Drive Edwardsburg, MI 49112 (269) 699-7182 phone & fax ------------------------------ Message: 2 Date: Wed, 21 Jul 2004 12:11:08 -0500 From: "Johnson, Teri" Subject: [Histonet] adhesive tape & MMA Sections - Turpentine? To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Patsy was describing the technique for using the paraffin tape transfer system on MMA blocks. One needs to remove the tape with an aliphatic or aromatic hydrocarbon before hydration and subsequent staining. Phillip was describing tape transfer method for cryosections. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org ------------------------------ Message: 3 Date: Wed, 21 Jul 2004 13:39:10 -0400 From: "Roberta Horner" Subject: [Histonet] Agarose blocks To: Message-ID: <00a801c46f49$a1dd7f00$8861ba92@padlspsu.psu.edu> Content-Type: text/plain; charset="US-ASCII" I just received tissue in agarose and I tried cutting a couple of them with very little luck. I have never worked with this before. Is there a trick to sectioning it? Should it have been processed differently? I didn't know what was in the block until I embedded it. Thank you for any help. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University ------------------------------ Message: 4 Date: Wed, 21 Jul 2004 12:37:12 -0500 From: "Poteete, Jacquie A." Subject: RE: [Histonet] Disposing of expired IHC reagents: Survey To: 'Joe Nocito' , Histonet Message-ID: Content-Type: text/plain Our hospital Safety Department prefers that we use the biohazard waste system for antibody and detection system reagent disposal. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com > -----Original Message----- > From: Joe Nocito [SMTP:JNocito@Pathreflab.com] > Sent: Wednesday, July 21, 2004 11:49 AM > To: Histonet > Subject: [Histonet] Disposing of expired IHC reagents: Survey > > Hey 'netters, > Need a little info if you please. How are you disposing of expired primary > antibodies and detection reagents? In the trash? Biohazard? Drain? Big > bonfire? > > > Joe Nocito, BS, HT(ASCP) QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp ------------------------------ Message: 5 Date: Wed, 21 Jul 2004 23:47:41 +0600 From: Subratab Subject: [Histonet] formalin nomenclature To: Message-ID: <200407211749.i6LHnaCa027768@mailout.proshikanet.com> Content-Type: text/plain; charset="iso-8859-1"; Dear all Here is a nice letter regarding the nomenclature of formalin: Manoonkitiwongsa PS, Schultz RL. Proper nomenclature of formaldehyde and paraformaldehyde fixatives for histochemistry. Histochem J. 2002 Jun-Jul;34(6-7):365-7. This paper is nice-reading and its helpfull to avoid discrepancy in describing fixatives in literature. Subrata Biswas, MD Dept of Nephrology University of Campinas SP, Brazil. ------------------------------ Message: 6 Date: Wed, 21 Jul 2004 13:58:11 -0400 From: "Flynn, Evelyn" Subject: RE: [Histonet] Blood vessel antibodies for rabbit models To: degaboh@rice.edu, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Dear Zarana, Some years ago I purchased a polyclonal goat anti-human Factor VIII (von Willebrand) which cross-reacted with rabbit, from Incstar Corporation. The staining with FFPE sections was very satisfactory. However, I have been unable to purchase more from this company, and I would love to find a new supplier. Regards, Evelyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of degaboh@rice.edu Sent: Tue 7/20/2004 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blood vessel antibodies for rabbit models Hello all, I'd like to use rack the brains of all you histonetters. Can anyone recommend antibodies to CD31 and/or CD34 that can be used in rabbit models? Or, can anyone recommend other antibodies for blood vessels that can be used in rabbit models? I'm having a hard time finding these. Thanks! Zarana Patel degaboh@rice.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 21 Jul 2004 13:24:20 -0500 From: "Sarah Jones" Subject: Re: [Histonet] Oil of wintergreen To: , Message-ID: Content-Type: text/plain; charset=US-ASCII The best thing for odors is X-O odor neutralizer. http://www.xocorp.com/ Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> 7/21/2004 7:04:55 AM >>> Does anyone know where oil of wintergreen can be purchased? The PA's at our facility used to use this product in the morgue to take care of odors while working on an autopsy. I cannot locate the product and was wondering if anyone could help me or what else is recommended? This is not an area that I am familiar with and am fairly new to my position here!! Thanks ahead of time for any and all help!! Dorothy Webb Regions Hospital St.Paul, Mn. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 21 Jul 2004 13:38:19 -0500 From: "Sarah Jones" Subject: Re: [Histonet] AURANTIA To: , Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Aurantia is a dye. Color Index number 10360. Try looking for it's synonym: Imperial Yellow. A German catalog identifies it as CI 18110 (benzol fast red B, FIAT 764). The free amine 2,2' ,4,4' ,6,6'-hexanitrodiphenylamine appears in chemical catalogs in reagent grade. From Conn's Biological Stains. I have several old vials of the dye. I can send you one if you can't find it. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> Jose Luis Palazon Fernandez 7/21/2004 4:59:58 AM >>> Dear Histonet fellows Do any of you know the comertial name of "Aurantia"? I need this reagent but I can?t find it in the chemical product catalogs. thanks in advance Jos? Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 21 Jul 2004 19:44:00 +0100 From: "Carl" Subject: [Histonet] cryostat sectioning of unfixed human brains To: Message-ID: <002201c46f52$b07f2320$d1039a51@home> Content-Type: text/plain; charset="iso-8859-1" Be most grateful for Standard operating procedures/protocols/ info to set up system for the freezing of unfixed human brain material and cutting of cryostat sections therefrom. The tissue is not known to be infected but I appreciate that such tissue has to be treated as potentially infected. Thank you --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 ------------------------------ Message: 10 Date: Wed, 21 Jul 2004 13:47:22 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Agarose blocks To: "Roberta Horner" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" What kind of microtome are you using? Soft things need to be cut with a Vibratome. How thin does it need to be? Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Wednesday, July 21, 2004 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Agarose blocks I just received tissue in agarose and I tried cutting a couple of them with very little luck. I have never worked with this before. Is there a trick to sectioning it? Should it have been processed differently? I didn't know what was in the block until I embedded it. Thank you for any help. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 21 Jul 2004 13:51:47 -0500 From: "Charles Scouten" Subject: RE: [Histonet] DECONTAMINATING CRYOSTATS To: "Winters, Bert" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I do not thing such a thing is even theoretically possible. Kill TB germs buried in ice? Get a self decontaminating cryostat, and do the job overnight while you sleep. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Winters, Bert Sent: Wednesday, July 21, 2004 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DECONTAMINATING CRYOSTATS Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 21 Jul 2004 15:02:28 -0400 From: Denise Crowley Subject: [Histonet] Webmaster attention To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" I was trying to log in with password "digest" to change to a digest format and this error message came up. thanks, Denise Bug in Mailman version 2.1.3 We're sorry, we hit a bug! If you would like to help us identify the problem, please email a copy of this page to the webmaster for this site with a description of what happened. Thanks! Traceback: Traceback (most recent call last): File "/home/mailman/scripts/driver", line 87, in run_main main() File "/home/mailman/Mailman/Cgi/options.py", line 206, in main password, user): File "/home/mailman/Mailman/SecurityManager.py", line 219, in WebAuthenticate print self.MakeCookie(ac, user) File "/home/mailman/Mailman/SecurityManager.py", line 228, in MakeCookie raise ValueError ValueError Python information: Variable Value sys.version 2.2.2 (#1, Feb 24 2003, 19:13:11) [GCC 3.2.2 20030222 (Red Hat Linux 3.2.2-4)] sys.executable /usr/bin/python sys.prefix /usr sys.exec_prefix /usr sys.path /usr sys.platform linux2 Environment variables: Variable Value CONTENT_LENGTH 53 CONTENT_TYPE application/x-www-form-urlencoded HTTP_REFERER http://lists.utsouthwestern.edu/mailman/options/histonet/dencrowl%40mit.edu SCRIPT_FILENAME /home/mailman/cgi-bin/options PYTHONPATH /home/mailman SERVER_SOFTWARE Apache/2.0.40 (Red Hat Linux) SERVER_ADMIN root@localhost SCRIPT_NAME /mailman/options SERVER_SIGNATURE Apache/2.0.40 Server at lists.utsouthwestern.edu Port 80 REQUEST_METHOD POST HTTP_HOST lists.utsouthwestern.edu PATH_INFO /histonet SERVER_PROTOCOL HTTP/1.1 QUERY_STRING REQUEST_URI /mailman/options/histonet HTTP_ACCEPT */* PATH_TRANSLATED /var/www/html/histonet HTTP_USER_AGENT Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en-us) AppleWebKit/85.7 (KHTML, like Gecko) Safari/85.7 HTTP_CONNECTION close SERVER_NAME lists.utsouthwestern.edu REMOTE_ADDR 18.114.1.35 REMOTE_PORT 59068 HTTP_ACCEPT_LANGUAGE en-us, ja;q=0.21, de-de;q=0.86, de;q=0.79, fr-fr;q=0.71, fr;q=0.64, nl-nl;q=0.57, nl;q=0.50, it-it;q=0.43, it;q=0.36, ja-jp;q=0.29, en;q=0.93, es-es;q=0.14, es;q=0.07 UNIQUE_ID Aj1mO8elmKIAADLdXUkAAAAI SERVER_PORT 80 GATEWAY_INTERFACE CGI/1.1 SERVER_ADDR 199.165.152.162 DOCUMENT_ROOT /var/www/html ------------------------------ Message: 13 Date: Wed, 21 Jul 2004 14:04:32 -0500 From: "Charles Scouten" Subject: RE: [Histonet] DECONTAMINATING CRYOSTATS To: "Winters, Bert" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I forgot to mention, full decontamination can be done in 3.5 hours, frozen to frozen, with thaw and decontamination steps in between. And no user interaction. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Winters, Bert Sent: Wednesday, July 21, 2004 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DECONTAMINATING CRYOSTATS Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 21 Jul 2004 14:26:25 -0500 From: "Charles Scouten" Subject: RE: [Histonet] (Histonet) Sakura Auto Stainer To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Why not look for a stainer with 44 baths. myNeurolab.com offers the most versatile Tissue stainer in TST 44. it comes with 44 baths and will allow you to do up to 400 slides per minute. You can obtain more information about this product at the followign link. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=545201&catdesc=Histology+Equipment&CatThreeID=648&CatOneID=4&subcatdesc=Tissue+Staining&idsubcategory=192 If you have any question or would like a demonstration please contact us. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Wilson Sent: Friday, July 16, 2004 11:48 PM To: histonet@pathology.swmed.edu Subject: [Histonet] (Histonet) Sakura Auto Stainer I am currently considering purchasing a Sakura Automatic slide stainer- DRS 6000 (i think). I would like to set this instrument up to stain both Histology and Cytology slides. I understand the unit has only 27 resevoirs for reagents, but can any of the resevoirs be commonly used by both procedures? Or, to get to the point- does anyone do this with their stainer and would they be willing to share the procedure? Thanks Jerry Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 21 Jul 2004 15:38:00 -0400 From: james.zimmerman@pharma.novartis.com Subject: [Histonet] C4D To: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Does anyone have any experience with Compliment 4 D? If so, I would appreciate a vendor and protocol in monkey and/or mice. Thanks, JPZ ------------------------------ Message: 16 Date: Wed, 21 Jul 2004 15:42:29 -0500 From: "Mitchell \(Jean\)" Subject: [Histonet] Darkroom Processor To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Is anyone familiar with or currently using a MohrPro 8 Darkroom Processor? I am looking for some feedback on this instrument before making the purchase. Thanks, Jean Mitchell University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI ------------------------------ Message: 17 Date: Wed, 21 Jul 2004 15:06:09 -0500 From: "Sarah Jones" Subject: [Histonet] hard paraffin To: Message-ID: Content-Type: text/plain; charset=US-ASCII What is the hardest paraffin on the market? I've found Gold Standard Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have any comments about the Gold Standard paraffins? Years ago, a lab used some sort of additive to their paraffin for bone that made the block yellow in color. Does anyone know what that could have been. I believe it was something like picrotin? Ring any bells? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 ------------------------------ Message: 18 Date: Wed, 21 Jul 2004 17:00:33 -0400 From: "Connolly, Brett M" Subject: [Histonet] brightfield 3D reconstruction To: "'HISTONET' (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain Aside from 3D reconstruction of confocal, fluorescent images, is anyone aware of any software/hardware for doing 3D reconstruction on serial sections of brightfield images? I have heard of VoxBlast from Vaytek, but that's about it. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ ------------------------------ Message: 19 Date: Wed, 21 Jul 2004 15:11:05 -0600 From: Gayle Callis Subject: [Histonet] hard paraffin additive To: "Sarah Jones" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.0.20040721150518.01b1fa18@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Tissue Prep 2 is hard but its melting point is not that high, and check with Richard Allan - I think they have one also. I think you were thinking of piccolyte, a resin that dissolves in the melted paraffin. We add this to paraffin milleniums ago, before paraffin technology and additives improved. It made the paraffin supersticky plus it was a total pain to deal with, a huge mess. I recall the company that made it was called Hercules Corp, I am not sure you can even get it anymore. It come in huge bags and big chunks in the bag. There is a publication on this resin in J of Histotechnology back a few years. Personally, you should try to find one ready to go to save time. Surgipath has a blue ribbon paraffin, check with them also. At 02:06 PM 7/21/2004, you wrote: >What is the hardest paraffin on the market? I've found Gold Standard >Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have >any comments about the Gold Standard paraffins? Years ago, a lab used >some sort of additive to their paraffin for bone that made the block >yellow in color. Does anyone know what that could have been. I believe >it was something like picrotin? Ring any bells? Thanks, Sarah > >Sarah Jones HT(ASCP) >Dept. of Vet. Anatomy & Public Health >Histology Lab >Texas A&M University >College Station, TX 77843-4458 >phone: 979-845-3177 >fax: 979-458-3499 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 20 Date: Wed, 21 Jul 2004 15:17:58 -0600 From: Gayle Callis Subject: Re: [Histonet] brightfield 3D reconstruction To: "Connolly, Brett M" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.0.20040721151203.01b2ef90@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed The publication, Microscopy Today frequently has lpublications on this technology. You can check out their website and do a search. Also, people who do confocal and associated with core imaging facilities probably do 3D reconstruction are easily accessed via confocal listserver out of Buffalo, ask the pertinent question. (you may already participate?) At 03:00 PM 7/21/2004, you wrote: >Aside from 3D reconstruction of confocal, fluorescent images, is anyone >aware of any software/hardware for doing 3D reconstruction on serial >sections of brightfield images? I have heard of VoxBlast from Vaytek, but >that's about it. > >Thanks, Brett Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 21 Date: Wed, 21 Jul 2004 14:30:46 -0700 From: Rocan Subject: Re: [Histonet] Blood vessel antibodies for rabbit models To: "'histonet@pathology.swmed.edu'" , "Flynn, Evelyn" Message-ID: <3A27E75C-DB5D-11D8-8142-000A9589219E@mac.com> Content-Type: text/plain; charset=US-ASCII; format=flowed I think there is a goat polyclonal against human vwf is now available through DAKO. Indeed this antibody stains vessels from human and mouse and I would expect it to cross also with rabbit. ----- Dr.Rocio Sierra-Honigmann Director Engineered Wound Repair Laboratory Cedars Sinai Research Institute Davis 1091 310-423-1882 Honigmannr@cshs.org On Jul 21, 2004, at 10:58 AM, Flynn, Evelyn wrote: > Dear Zarana, > Some years ago I purchased a polyclonal goat anti-human Factor > VIII (von Willebrand) > which cross-reacted with rabbit, from Incstar Corporation. The > staining with FFPE > sections was very satisfactory. However, I have been unable to > purchase more from > this company, and I would love to find a new supplier. > > Regards, > Evelyn > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > degaboh@rice.edu > Sent: Tue 7/20/2004 5:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Blood vessel antibodies for rabbit models > > Hello all, > > I'd like to use rack the brains of all you histonetters. Can anyone > recommend antibodies to CD31 and/or CD34 that can be used in rabbit > models? > Or, can anyone recommend other antibodies for blood vessels that can > be used > in rabbit models? I'm having a hard time finding these. > > Thanks! > > Zarana Patel > degaboh@rice.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 21 Jul 2004 14:57:04 -0700 From: "Brody,Juanita X" Subject: [Histonet] Indirect Immunofluorescence To: "'histonet@pathology.swmed.edu'" Message-ID: <8220AFE580A4A34582D63C3D3B2C00EB0F60EF@cscrdemsg004.crdc.kp.org> Content-Type: text/plain; charset="iso-8859-1" We do indirect IF on skin specimens and having problems with false positives and background staining. Is there any one doing this procedure who would be willing to share the source of money slides, control sera, and procedure? Thanks in advance. ------------------------------ Message: 23 Date: Wed, 21 Jul 2004 20:01:11 -0400 From: Subject: Re: [Histonet] DECONTAMINATING CRYOSTATS To: "Winters, Bert" , Message-ID: <008a01c46f7f$00df9940$a623d445@domainnotset.invalid> Content-Type: text/plain; charset="iso-8859-1" How about not allowing frozen sections on lung for the diagnosis of infectious diseases? TB, Pneumocystis, etc. Do a touch prep for the fresh tissue, and stain the slide that way. Make several slides, so can do H&E, Giemsa, GMS, Kinyoun, PASH, whatever is needed. Fix and process the tissue used for the touch prep, for diagnosis on a permanent section the next day. Most of the time, the touch prep will reveal the micro-organisms. Once in a great while, there is a false negative, which is picked up the next day on the fixed processed block. If it is explained to the surgeons that doing frozen sections on infectious lung tissue puts the cryostat out of commission for the rest of the day, and that no other surgeries, including all tumor cases, will have any FS done for the rest of that day, they are usually willing to accept the results of the touch preps. Occasionally, an infectious lung will still slip through, but usually because they thought the x-ray showed a tumor not an infection, or there is infection with the tumor. But there is no need to do a FS on an infectious case. Like you said, it decommissions the cryostat, to say nothing of the potential biohazardous risk the person doing the sectioning is in, if they accidentally cut themselves. You will need the support/backing of the pathologists. But they should support you, knowing that the cryostat won't be available for the rest of the day. (Even if they aren't worried about the risk to the sectioner.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Winters, Bert" To: Sent: Wednesday, July 21, 2004 11:52 AM Subject: [Histonet] DECONTAMINATING CRYOSTATS Lately we have done several frozen sections on lung cases that have turned out to be possible T.B. cases. We then have to shut down our cryostats, defrost them and decontaminate them Does anyone know of any decontamination procedures that does not require the total defrosting of the cryostat. Bert Winters, Northwest community hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Wed, 21 Jul 2004 18:02:15 -0700 From: Swaram Subject: [Histonet] Pan Melanoma cocktail from "Not" Mouse...?? To: HISTONET Message-ID: <40FF1217.5090905@myrealbox.com> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, I am in need of Pan Melanoma cocktail from any species other than Mouse. I have already tried one other Ab (polyclonal Rabbit Mart-1/Melan A) and it did not do a good job compared to the Pan Melanoma cocktail (containing HMB-45, MART-1 & Tyrosinase). The Ab should is to be used for IF and on paraffin fixed tissue. Anybody who makes them, kindly contact me. Any information from histonetters would be appreciated. Thanks, Swaram. ------------------------------ Message: 25 Date: Wed, 21 Jul 2004 20:57:14 -0500 From: "Sarah Jones" Subject: Re: [Histonet] hard paraffin additive To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Thanks for your reply Gayle. From the info you gave, I found piccolyte on the Hercules web site. Still looking for the J of H article. Wouldn't paraffin hardness be a factor of melting point, with the higher melting point being the harder paraffin? Thanks, Sarah >>> Gayle Callis 7/21/2004 4:11:05 PM >>> Tissue Prep 2 is hard but its melting point is not that high, and check with Richard Allan - I think they have one also. I think you were thinking of piccolyte, a resin that dissolves in the melted paraffin. We add this to paraffin milleniums ago, before paraffin technology and additives improved. It made the paraffin supersticky plus it was a total pain to deal with, a huge mess. I recall the company that made it was called Hercules Corp, I am not sure you can even get it anymore. It come in huge bags and big chunks in the bag. There is a publication on this resin in J of Histotechnology back a few years. Personally, you should try to find one ready to go to save time. Surgipath has a blue ribbon paraffin, check with them also. At 02:06 PM 7/21/2004, you wrote: >What is the hardest paraffin on the market? I've found Gold Standard >Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have >any comments about the Gold Standard paraffins? Years ago, a lab used >some sort of additive to their paraffin for bone that made the block >yellow in color. Does anyone know what that could have been. I believe >it was something like picrotin? Ring any bells? Thanks, Sarah > >Sarah Jones HT(ASCP) >Dept. of Vet. Anatomy & Public Health >Histology Lab >Texas A&M University >College Station, TX 77843-4458 >phone: 979-845-3177 >fax: 979-458-3499 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Thu, 22 Jul 2004 08:54:02 +0100 From: "Brett, Lawrence" Subject: RE: [Histonet] Galectin-3 To: "'Sawrenko, Christina'" , "Histonet (E-mail)" Message-ID: <857344E7D8F90240935A288998A89D811E774B@wgh-ex1.luht.scot.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" Yes we use GAL 3 for Thyroid tumours too, along with Cytokeratin 19. Papillary Ca's are positive with CK19 and mostly negative with Gal 3 while follicular ca's are mostly positive with GAL 3 and mostly negative with CK19. See Beesley & McLaren. Histopathology 41. 3. p236 (2002). Lawrence Brett Royal Infirmary Edinburgh Scotland -----Original Message----- From: Sawrenko, Christina [mailto:csawrenk@bccancer.bc.ca] Sent: 21 July 2004 22:07 To: Brett, Lawrence Subject: RE: [Histonet] Galectin-3 Thanks for the info. For what application do you use the antibody? We are interested in using it for thyroid neoplasms. Thanks again, Chris -----Original Message----- From: Brett, Lawrence [ mailto:Lawrence.Brett@luht.scot.nhs.uk ] Sent: Wednesday, July 21, 2004 8:11 AM To: 'Sawrenko, Christina' Subject: RE: [Histonet] Galectin-3 We use Novocastra's Galectin 3 but not with CD44v6. Gal 3 works well with Tris-EDTA heat antigen retrieval. We use a microwave pressure cooker (Biogenex) and incubate for 30 mins in primary diluted at 1:150. Hope this is of some value. Lawrence Brett Immunocytochemistry Lab. Royal Infirmary Edinburgh Scotland -----Original Message----- From: Sawrenko, Christina [ mailto:csawrenk@bccancer.bc.ca ] Sent: 16 July 2004 18:21 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Galectin-3 Good morning, One of our pathologists has asked us to research Galectin-3 for use in thyroid neoplasms. Does anyone have experience with this antibody and do you use it in conjuction with CD44v6 (Novocastra)? Thanks in advance for your help! Chris Sawrenko Histopathology BC Cancer Agency Vancouver BC Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** The information contained in this message may be confidential or legally privileged and is intended for the addressee only, If you have received this message in error or there are any problems please notify the originator immediately. The unauthorised use, disclosure, copying or alteration of this message is strictly forbidden. ********************************************************************** ********************************************************************** The information contained in this message may be confidential or legally privileged and is intended for the addressee only, If you have received this message in error or there are any problems please notify the originator immediately. The unauthorised use, disclosure, copying or alteration of this message is strictly forbidden. ********************************************************************** ------------------------------ Message: 27 Date: Thu, 22 Jul 2004 09:46:53 +0100 From: Margaret Blount Subject: RE: [Histonet] Agarose blocks To: 'Roberta Horner' , histonet@lists.utsouthwestern.edu Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138C@mius2.medlan.cam.ac.uk> Content-Type: text/plain; charset="iso-8859-1" I have cut lots of agar blocks and used the normal tissue processing schedules of whichever lab I worked in. I usually post fixed the agar block in Neutral Buffered Formalin for a few hours to a day after embedding depending upon how well fixed the sample was prior to embedding in the agar and processing. I do think your client ought to have told you what was embedded in the agarose else how do you know how much to trim or what the orientation is? I have used this procedure to orientate isolated hair follicles and flat intestine samples so that I could get sections of precise areas, so it was essential to have all the available information about the sample. In difficult cases, I hand processed the agar blocks to paraffin, but this should not be necessary if you have a process that is suited to the tissue sample itself. I always used 2% w/v aqueous agar (Difco) and maintained it at 55 to 60C in a waterbath to keep it molten. I made a mould suited to the sample, e.g. a cut off syringe, pipetted a layer of agar into it and allowed it to start gelling, I then layered on my sample so as to orientate it correctly, then pipetted a few more drops of agar on top, taking care to allow it to cool sufficiently to avoid "cooking" my sample. To achieve the latter, I drew up some agar into a pastette and held it at room temperature for a few seconds to allow it to cool, trial and error taught me how long to do this. I have to say that I have used agar for both paraffin and frozen sectioning successfully. My hand process went through 70% ethanol, 3 changes of absolute ethanol and 1 of histoclear each step being 1 hour, then overnight in histoclear, followed by a further histoclear of 1 hour, 2 changes of wax in the wax oven for 1 hour then embed. This worked well for me. I hope this helps. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Roberta Horner [mailto:rjr6@psu.edu] Sent: Wednesday, July 21, 2004 6:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Agarose blocks I just received tissue in agarose and I tried cutting a couple of them with very little luck. I have never worked with this before. Is there a trick to sectioning it? Should it have been processed differently? I didn't know what was in the block until I embedded it. Thank you for any help. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 28 Date: Thu, 22 Jul 2004 09:49:50 +0100 From: Margaret Blount Subject: RE: [Histonet] brightfield 3D reconstruction To: "'Connolly, Brett M'" , "'HISTONET' (histonet@lists.utsouthwestern.edu)" Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138D@mius2.medlan.cam.ac.uk> Content-Type: text/plain; charset="iso-8859-1" I'm not sure if I'm right but I think that Olympus' software, AnalySIS can do this, why not contact your Olympus rep? Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Connolly, Brett M [mailto:brett_connolly@merck.com] Sent: Wednesday, July 21, 2004 10:01 PM To: 'HISTONET' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] brightfield 3D reconstruction Aside from 3D reconstruction of confocal, fluorescent images, is anyone aware of any software/hardware for doing 3D reconstruction on serial sections of brightfield images? I have heard of VoxBlast from Vaytek, but that's about it. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ---------------------------------------------------------------------------- -- Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ---------------------------------------------------------------------------- -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 32 *************************************** From yhwang0731 <@t> hotmail.com Thu Jul 22 23:30:22 2004 From: yhwang0731 <@t> hotmail.com (Yi-hsin Wang) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] staining compatibility Message-ID: Hi everyone, I am a new subscriber, am glad there are so many experts around can offer help. I am a medical student doing summer research. We use simplified H&E stain before LCM (Laser capture microdissection) with subsequent proteomic analysis. I stumbled upon this journal: Electrophoresis 2003, 24, 296-302, which claims that conventional histological staining methods are not compatible with 2-dimentional gel electrophoresis. While another paper: American Journal of Pathology 2002, 160:815-822 claims that those histochemical stains is compatible with such protein analysis, variations for the relative intensity and reproducibility of proteins is observed, though. These two opinions seems to me a little contradictory and I am currently working on it, trying to establish a master gel which other Laser captured gel can compare to. Any suggestion is highly welcome, thanks in advance. ---------------------------------------------------------------- Yi-hsin Wang Department of Medicine Kaohsiung Medical University 100 Shi-Chuan 1st Road, San Ming District, Kaohsiung City, Taiwan yhwang0731@hotmail.com _________________________________________________________________ The new MSN 8: smart spam protection and 2 months FREE* http://join.msn.com/?page=features/junkmail From mab70 <@t> medschl.cam.ac.uk Fri Jul 23 02:58:33 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Microscope filters Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138F@mius2.medlan.cam.ac.uk> Dear Geoff, Thanks, that is a great help. My colleague isn't actually using film as he has a digital camera, but I will do a s you suggest and contact the microscope manufacturers for trial samples. I can easily pop into Jessops any time. Thanks again Margaret -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Thursday, July 22, 2004 7:44 PM To: Margaret Blount Cc: Histonet (E-mail) Subject: Re: [Histonet] Microscope filters Hi Margaret: Assuming your colleague is using black and white negative film, a blue filter will pass more blue light to the film and give more density in the negative. When printed, that will translate to the blue subject being lighter on the print. That said, there are lots of blue filters used in photography and some trial and error may be needed. There are lots of different "daylight filters", depending on the light source and the amount of correction needed, an 80A filter might be blue enough for your application. The standard blue filter for making RGB separations is a #47 and it is a fairly dense blue-purple color. A #46 is very similar. Such filters are sold in photo stores that cater to professional photographers (Jessop's in the UK is one) but you might be able to find something in the AudioVisual dept at your institution or perhaps the manufacturer of your microscope might loan you several for a trial, you could then purchase the one that fits your needs. Geoff Margaret Blount wrote: >Hi all, > >I have a chinese colleague who requires a blue filter to suppress staining >for Nissl substance (violet) in brightfield transmitted light microscopy - >does anyone know what filter this is and a supplier of such a filter. So far >I have only identified a Daylight filter, but I am not sure if this would do >the trick or not. If anyone knows anything about this I would be very >grateful as would my colleague. > >Thanks in anticipation. > >Margaret > >Margaret Blount >Chief Technician >Clinical Biochemistry >University of Cambridge >Addenbrooke's Hospital >Hills Road >Cambridge >CB2 2QR > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From mab70 <@t> medschl.cam.ac.uk Fri Jul 23 03:04:32 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] (no subject) Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111391@mius2.medlan.cam.ac.uk> Go to the link at the bottom of the message and you will find all the instructions on the web page. margaret -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Thursday, July 22, 2004 5:31 PM To: Histonet (E-mail) Subject: [Histonet] (no subject) Please tell how to unsubscribe. I'm going on vacation and will be turning on my "Out of Office" message. I have tried to unsubscribe to no avail. Charlene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Jul 23 05:52:50 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Repetitive motion Syndrome in particular use ofshoulders. References: <20040722202626.70565.qmail@web20805.mail.yahoo.com> Message-ID: <001a01c470a3$34015820$dd2fd445@domainnotset.invalid> See the following for some information on RMS and laboratory work. University of Minnesota Environmental Health and Safety Laboratory Ergonomics http://www.dehs.umn.edu/ergo/lab/ Prevention of Musculoskeletal Symptoms Among Histotechnologists by using Ergonomics And Biomechanical Analysis http://www.prevencionintegral.com/Articulos/@Datos/02_104.htm NIEHS (National Institute of Environmental Health and Safety, part of NIH (National Institutes of Health)): Health and Safety Guide to Laboratory Ergonomics http://www.niehs.nih.gov/odhsb/ergoguid/home.htm CDC (Center for Disease Control): Laboratory Ergonomics http://www.cdc.gov/od/ohs/Ergonomics/labergo.htm I think the information from NIH and CDC would be your strongest source of information, having the most clout. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Janice McDonald" To: Sent: Thursday, July 22, 2004 4:26 PM Subject: [Histonet] Repetitive motion Syndrome in particular use ofshoulders. > I sustained an injury to my left shoulder while at > work in the lab when I fell off a defective stool and > landed on the floor hitting my shoulder. The insurance > company is refusing to pay for my treatment as their > Occupational Specialist has stated that histology work > is not repetitive and in particular the shoulder is > not affected.They have requested that I provide > information to the contrary if I wish to have my case > reviewed.We were using an older model Microm where the > left handle came 4/5ths of the way to the top of the > microtome which required my arm to be in a slightly > raised position causing me considerable discomfort.The > lab is a research lab at a medical school and all our > staining and coverslipping were done manually in a > large fume hood that had a lowered glass door. > I am hoping that some-one knows or has on file any > documented information that they can send to me > relating to RMS in particular mentioning the shoulder > that I can present it to the insurer. > Thanks ! > Jan McDonald > histokiwi@yahoo.com > Skin Institute > Lake Rd, > Takapuna > Auckland > New Zealand. > > > > __________________________________ > Do you Yahoo!? > Yahoo! Mail - 50x more storage than other providers! > http://promotions.yahoo.com/new_mail > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Xudong_Cao <@t> Brown.edu Fri Jul 23 08:01:30 2004 From: Xudong_Cao <@t> Brown.edu (Cao, Xudong) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining? Message-ID: <1DA88DDC48CCC245A777599FDAEED6D0A3905B@MAIL1.AD.Brown.Edu> Dear all, I am trying to stain for nerve fibers innervating the skin samples that I have. While I am lucky to get the nerve fiber singnals, I am also getting overwheelingly strong background staining from the hair follicles. I know the follicle stains are background since they are also stained in both of my controls where primary and secondary are omitted are also stained. My question is: how to get rid of the hair follicle stains? any suggestions? I am using Vector's ABC and SG substrate. Thanks Xudong Cao, Ph.D. Biomedical Center Brown University From David.Edmondson <@t> christie-tr.nwest.nhs.uk Fri Jul 23 08:56:23 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining? Message-ID: Have you tried applying a biotin blocking kit in the procedure? Dave Christie, Manchester -----Original Message----- From: Cao, Xudong [mailto:Xudong_Cao@Brown.edu] Sent: 23 July 2004 14:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining? Dear all, I am trying to stain for nerve fibers innervating the skin samples that I have. While I am lucky to get the nerve fiber singnals, I am also getting overwheelingly strong background staining from the hair follicles. I know the follicle stains are background since they are also stained in both of my controls where primary and secondary are omitted are also stained. My question is: how to get rid of the hair follicle stains? any suggestions? I am using Vector's ABC and SG substrate. Thanks Xudong Cao, Ph.D. Biomedical Center Brown University From BSylinda <@t> aol.com Fri Jul 23 09:36:30 2004 From: BSylinda <@t> aol.com (BSylinda@aol.com) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] E-TX Histotechnologist Wanted Message-ID: <7FC6EFD9.5B047F4F.00698496@aol.com> Immediate opening for Histology Technologist with HT or HTL ASCP Certifications. The full-time position requires experience in basic histology functions, grossing, immunohistochemical and routine stains. Relocation allowance, flexible vacation schedule, competitive salary and excellent pension plan. We are located in beautiful East Texas with the availability of many recreational areas. The 60,000 population town has a low cost of living, low crime rate, excellent schools and no state income tax. The laboratory is pathologist-owned. Please send resume to Pathology Services of Texarkana, attention: Leah Barber, Office Manager email: pathservices@cableone.net fax: 903-793-2332 mail: 1002 Texas Blvd., Ste. 500 Texarkana, TX 75503 903-792-1331 From giorgia.setti <@t> kcl.ac.uk Fri Jul 23 09:45:20 2004 From: giorgia.setti <@t> kcl.ac.uk (Giorgia Setti) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] (no subject) Message-ID: <1121528449-301581@pathology.swmed.edu> Giorgia Setti mailto:giorgia.setti@kcl.ac.uk Dear histonetters!!!, does anybody know a good antibody for rat MCP-1 and if MCP-1 is expressed in rat spleen?? Thank you Giorgia From michael_lafriniere <@t> memorial.org Fri Jul 23 10:10:39 2004 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] HT/HTL Review Session Message-ID: Last call for Region III with the Georgia State Society HT/HTL Review Session Registration We have a few openings left for a HT/HTL review session : pre registration is a must...there will be no onsite registration. DATE: Sat. August 14th Time : 8am-5pm Place: Atlanta, Georgia Instructors: Shirley Powell, Mercer University School of Medicine Macon GA & Carl Sagasser, Darton College Albany GA Lunch provided by Sakura Cost $75.00 Email: Michael LaFriniere, NSH Region III Director to obtain registration form and directions. Michael_lafriniere@memorial.org Phone:423-495-6117 This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. "Sections 2510-2521", and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Warren_Eddings <@t> ssmhc.com Fri Jul 23 10:16:47 2004 From: Warren_Eddings <@t> ssmhc.com (Warren_Eddings@ssmhc.com) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] immuno stainers Message-ID: Does anyone have knowledge about the bio care NEMESIS ? thanks warren okla city From livieira <@t> ualg.pt Fri Jul 23 10:10:38 2004 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] frozen samples Message-ID: <002e01c470c7$36513130$2914100a@labhistologia> Hi everyone Can someone give me some avise about the better method for processing that sanples: - frozen samples of fish gonads without freezing medium Can we expect make satisfatory cuts in cryostat? Can we defrost this samples and after process in paraffin? Thanks Lina Vieira From jerry.lyons <@t> ucd.ie Fri Jul 23 10:21:25 2004 From: jerry.lyons <@t> ucd.ie (Jeremiah Lyons) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Endothelial cell staining Message-ID: <003501c470c8$b7ebf1c0$6e892b89@DFFRCL0J> To whom it may concern, I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work in mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated. Jerry From jerry.lyons <@t> ucd.ie Fri Jul 23 10:18:38 2004 From: jerry.lyons <@t> ucd.ie (Jeremiah Lyons) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Endothelial cell staining Message-ID: <001e01c470c8$54584e60$6e892b89@DFFRCL0J> To whom it may concern, I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work in mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated. Jerry From jerry.lyons <@t> ucd.ie Fri Jul 23 10:23:24 2004 From: jerry.lyons <@t> ucd.ie (Jeremiah Lyons) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] endothelial cell staining Message-ID: <001001c470c8$fefdc2f0$6e892b89@DFFRCL0J> Hi, I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work on mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated. Jerry From jmitchell <@t> neurology.wisc.edu Fri Jul 23 10:42:29 2004 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean)) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] PGP9.5 staining -- how to get rid of hair folliclebackground staining? Message-ID: A couple of questions for you first: What is the thickness of your sections? What concentration of primary do you normally use? I do PGP 9.5 staining on 50 micron frozen sections of skin. The hair follicles will stain somewhat but should not be overwhelming. Jean Mitchell University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cao, Xudong Sent: Friday, July 23, 2004 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PGP9.5 staining -- how to get rid of hair folliclebackground staining? Dear all, I am trying to stain for nerve fibers innervating the skin samples that I have. While I am lucky to get the nerve fiber singnals, I am also getting overwheelingly strong background staining from the hair follicles. I know the follicle stains are background since they are also stained in both of my controls where primary and secondary are omitted are also stained. My question is: how to get rid of the hair follicle stains? any suggestions? I am using Vector's ABC and SG substrate. Thanks Xudong Cao, Ph.D. Biomedical Center Brown University From cfranci <@t> rigel.com Fri Jul 23 10:46:35 2004 From: cfranci <@t> rigel.com (Christian Franci) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Conundrum Message-ID: I'm in somewhat of a pickle... It seems that my PEFF sections keep floating away from the slides when I do HIER using my usual pressure cooker method in which they're submerged in buffer. Never had this problem before... (I've always used a pressure cooker. Have never tried other methods.) Can steaming them instead be a better way to avoid this? If so, how do you go about it? any tricks of the trade? My suspicion is that the formalin concentration that was used to fix the tissues was off. (Could that be the case?) Of course, these are precious study samples for a paper so... anyone have any ideas how I can save them? or am I out of luck? Much gratitude for any insights that might help! Cheers Chris ===== "Eliminate all other factors and, the one which remains MUST be the truth".-Sherlock Holmes- From MAUGER <@t> email.chop.edu Fri Jul 23 10:49:23 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] endothelial cell staining Message-ID: To Jerry, I have had good luck staining endothelium in mice with rat anti ms CD31 from Pharmingen(BD Biosciences) Catalog # is 557355. Jo >>> "Jeremiah Lyons" 07/23/04 11:23AM >>> Hi, I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work on mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated. Jerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Fri Jul 23 10:51:57 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Conundrum Message-ID: Dear Chris, Try post fixing the slides in NBF for 30 minutes after deparaffinizing.This has helped some tissue stay on for me in the past. Luck, Jo >>> "Christian Franci" 07/23/04 11:46AM >>> I'm in somewhat of a pickle... It seems that my PEFF sections keep floating away from the slides when I do HIER using my usual pressure cooker method in which they're submerged in buffer. Never had this problem before... (I've always used a pressure cooker. Have never tried other methods.) Can steaming them instead be a better way to avoid this? If so, how do you go about it? any tricks of the trade? My suspicion is that the formalin concentration that was used to fix the tissues was off. (Could that be the case?) Of course, these are precious study samples for a paper so... anyone have any ideas how I can save them? or am I out of luck? Much gratitude for any insights that might help! Cheers Chris ===== "Eliminate all other factors and, the one which remains MUST be the truth".-Sherlock Holmes- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerry.lyons <@t> ucd.ie Fri Jul 23 10:52:32 2004 From: jerry.lyons <@t> ucd.ie (Jeremiah Lyons) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Endothelial cell staining Message-ID: <002301c470cd$109e8f90$6e892b89@DFFRCL0J> Hi! I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work in mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated. Jerry From lizchlipala <@t> premierhistology.com Fri Jul 23 10:59:21 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] endothelial cell staining In-Reply-To: <001001c470c8$fefdc2f0$6e892b89@DFFRCL0J> Message-ID: <001501c470ce$081c1ad0$74d48a80@LIZ> Jerry You can use PECAM-1 (M-20) from Santa Cruz. It is a goat polyclonal and will work on formalin fixed paraffin embedded material. I have used it on mouse tissue with good results. I have images and a protocol available if you need one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeremiah Lyons Sent: Friday, July 23, 2004 8:23 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial cell staining Hi, I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work on mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated. Jerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Fri Jul 23 11:26:19 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Conundrum In-Reply-To: Message-ID: Christian, We have had recent problems with tissue floating off the slides after decloaking them and even after performing heat retrieval on the Ventana XT. We have tried many different slides and still continue to do so. The best that I've come across is from StatLab cat # 318. There's a catch to ordering them though. Their number is 800-442-3573. Tell them Joe sent you. I'm in the middle of testing some slides from Erie Scientific, but haven't completed the testing yet. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Christian Franci Sent: Friday, July 23, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Conundrum I'm in somewhat of a pickle... It seems that my PEFF sections keep floating away from the slides when I do HIER using my usual pressure cooker method in which they're submerged in buffer. Never had this problem before... (I've always used a pressure cooker. Have never tried other methods.) Can steaming them instead be a better way to avoid this? If so, how do you go about it? any tricks of the trade? My suspicion is that the formalin concentration that was used to fix the tissues was off. (Could that be the case?) Of course, these are precious study samples for a paper so... anyone have any ideas how I can save them? or am I out of luck? Much gratitude for any insights that might help! Cheers Chris ===== "Eliminate all other factors and, the one which remains MUST be the truth".-Sherlock Holmes- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rocan <@t> mac.com Fri Jul 23 11:33:55 2004 From: rocan <@t> mac.com (Rocan) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Endothelial cell staining In-Reply-To: <001e01c470c8$54584e60$6e892b89@DFFRCL0J> References: <001e01c470c8$54584e60$6e892b89@DFFRCL0J> Message-ID: <16B95C22-DCC6-11D8-AF6A-000A9589219E@mac.com> The Pharmigen antibody that Joanne mentioned is the best for mouse tissue. Frozen works much better than paraffin sections. We use thick sections (20-30um) to see whole microvascular networks. Long incubations make the results much, much better. We incubate overnight at room temperature! I don't know if your fixative may have obscured the epitope. You may need to do some antigen unmasking . Good Luck ----- Dr.Rocio Sierra-Honigmann Director Engineered Wound Repair Laboratory Cedars Sinai Research Institute Davis 1091 310-423-1882 Honigmannr@cshs.org On Jul 23, 2004, at 8:18 AM, Jeremiah Lyons wrote: > To whom it may concern, > > I'm a PhD student who is looking for advice on endothelial cell > staining in mouse tumour tissue for the purpose of microvessel density > staining.Most of the available antibodies don't work in mouse tissue. > At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) > with rabbit anti-human Von Willebrand factor. Any suggestions would be > greatly appreciated. > > Jerry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara_Lentz <@t> dahlchase.com Fri Jul 23 11:45:36 2004 From: Barbara_Lentz <@t> dahlchase.com (Barbara Lentz) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Conundrum Message-ID: We use the Ventana XT and place some of our slides in 10% NBF for 30 minutes. This helps the tissue stay on even when the slides haven't been rehydrated. Barb From gcallis <@t> montana.edu Fri Jul 23 14:19:44 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Carnoy's fixed tissue, Factor VIII Endothelial cell staining In-Reply-To: <001e01c470c8$54584e60$6e892b89@DFFRCL0J> References: <001e01c470c8$54584e60$6e892b89@DFFRCL0J> Message-ID: <6.0.0.22.0.20040723130612.01af41b8@gemini.msu.montana.edu> Carnoys (you can leave out chloroform since it is not a fixative, there to remove fat only, safer to not handle chlroroform) fixed mouse tissue, overnight only, rinse 2x with 95% alcohol, and process starting at 100% (since this is alcolhol based fixative, you can start processing at higher concentration alcohol, clear and embed in paraffin. DAKO, rabbit anti Human Factor VIII, von Willebrand factor, purified 1:200 to 1:250 for 30 minutes, Goat antirabbit or Donkey antiRAbbit-biotin - F(ab')2 frag of IgG from (Jackson). Make sure your secondary is adsorbed to mouse. At beginning, Endogenous peroxidase block, Normal serum block 5 - 10% matched to host of your secondary 30 min. Avidin/biotin block Primary antibody (30 - 60 min) RT, Secondary antibody-biotinylated approx 2 ug/ml, usually 1:250 for Donkey antirabbit biotin, from Jackson 30 min dilute your secondary in 1 - 5% normal serum matching host of secondary. Strepavidin-HRP (we do this in house dilution, Biosource/TAGO 5-6 mg/ml, diluted 1:500, for 20 min Choice of chromogen, we prefer AEC+ from DAKO Counterstain lightly with hematoxylin Use AquaMount with AEC to mount coverslip. Be sure to put 0.05% Tween 20 in all buffers and diluents. I advise doing a dilution panel on your primary to reach optimal primary antibody concentration starting at 10ug/ml - work on either side of that and choose best staining. You do not have to do any antigen retrieval with this method. Good luck and let us know how your staining turns out, we had great success with this protocol on murine tumor looking for angiogenesis. 09:18 AM 7/23/2004, you wrote: >To whom it may concern, > >I'm a PhD student who is looking for advice on endothelial cell staining >in mouse tumour tissue for the purpose of microvessel density >staining.Most of the available antibodies don't work in mouse tissue. At >the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with >rabbit anti-human Von Willebrand factor. Any suggestions would be greatly >appreciated. > >Jerry >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From asmith <@t> mail.barry.edu Fri Jul 23 16:05:41 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] unsubscribe Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3BA1@exchsrv01.barrynet.barry.edu> Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Rcartun <@t> harthosp.org Fri Jul 23 16:14:14 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] P504S Message-ID: Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun From JWEEMS <@t> sjha.org Fri Jul 23 16:29:50 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] P504S Message-ID: We are, but I didn't know it had that name! It is specific for prostate CA. Do you have technical questions or Dr. questions? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Friday, July 23, 2004 5:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P504S Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Barry.R.Rittman <@t> uth.tmc.edu Fri Jul 23 19:01:36 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] hard paraffin Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0635AF2@UTHEVS3.mail.uthouston.edu> Ceresin will increase the hardness of the wax. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sarah Jones Sent: Wed 7/21/2004 3:06 PM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] hard paraffin What is the hardest paraffin on the market? I've found Gold Standard Peel-A-Way with a melting point of 62-64 degrees C. Does anyone have any comments about the Gold Standard paraffins? Years ago, a lab used some sort of additive to their paraffin for bone that made the block yellow in color. Does anyone know what that could have been. I believe it was something like picrotin? Ring any bells? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rocan <@t> mac.com Sat Jul 24 13:14:40 2004 From: rocan <@t> mac.com (Rocan) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Endothelial cell staining-vWF-A word of caution In-Reply-To: <002301c470cd$109e8f90$6e892b89@DFFRCL0J> References: <002301c470cd$109e8f90$6e892b89@DFFRCL0J> Message-ID: <5496E3C6-DD9D-11D8-BC49-000A9589219E@mac.com> Jerry, No doubt that many histonet pros have great protocols for EC immunostaining. However, there is much controversy about vWF as a good endothelial marker. Please refer to this link: ww-klinik.uni-mainz.de/Pathologie/E/ZuStaffProfiles/ Kirkpatrick/PDF-Dateien/1_multipart_xF8FF_14_16-Kirk.pd. There are plenty of vascular biologist who would agree that vWF is not the best marker for microvessels not only in human tissue but in other species as well including mice (Dev Biol. 1991 Nov;148(1):51-62). Although vWF is an excellent marker for vein endothelium, it is less so for arteries. Venules and arterioles stain ok but capillary endothelium has at best a weak reaction and mostly does not have detectable vWF. This has much more to do with the biology of vWF than the quality of the antibodies. A colleague of mine had a manuscript rejected because he used vWF as EC marker in retina. The reviewers demanded the use of CD31. Furthermore, tumors can be negative to vWF in much of their vasculature. vWF can be used in addition to CD31 but never instead. CD34 also can be used to complement vWF since it is strongest in capillaries. However, no one would argue with a good CD31 stain which stains all vessels regardless of their caliber. Therefore, I think that for mouse tissue, the Pharmigen clone MEC13.3 (Eur J Cell Biol. 1994 Apr;63(2):247-54) is your best option. Professor Donald McDonald from UCSF has made a career on vascular imaging. I have learned so much from reading his papers and paying good attention to his protocols. You may want to go to start with this fantastic review and then go to specific protocols in his many publications. I assure you he is the best. McDonald DM, Choyke PL. Imaging of angiogenesis: from microscope to clinic. Nat Med. 2003 Jun;9(6):713-25 Good luck ----- Dr.Rocio Sierra-Honigmann Director Engineered Wound Repair Laboratory Cedars Sinai Research Institute Davis 1091 310-423-1882 Honigmannr@cshs.org On Jul 23, 2004, at 8:52 AM, Jeremiah Lyons wrote: > Hi! > > I'm a PhD student who is looking for advice on endothelial cell > staining in mouse tumour tissue for the purpose of microvessel density > staining.Most of the available antibodies don't work in mouse tissue. > At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) > with rabbit anti-human Von Willebrand factor. Any suggestions would be > greatly appreciated. > > Jerry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sun Jul 25 12:27:28 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] re agarose Message-ID: <003001c4726c$a8a61e70$df039a51@home> "Agarose is a linear polysaccharide (average molecular mas about 12,000) made up of the basic repeat unit agarobiose, which comprises alternating units of galactose and 3,6-anhydrogalactose. Agarose is usually used at concentrations between 1% and 3%." You don't say how you're cutting it, that I can see. I either embed in OCT, or similar, for frozen sectioning , or process to pwax. No need to fix prior to processing....for the above reasons --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.714 / Virus Database: 470 - Release Date: 02/07/2004 From jin_zhe <@t> hotmail.com Sun Jul 25 14:35:59 2004 From: jin_zhe <@t> hotmail.com (jin zhe) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Kcnq1 and kcne1 antibody Message-ID: Hello everyone, We are going to use polyclonal anti-Kcnq1 and Kcne1 antibody for our immunohistochemical study. There are several commecial antibodies from different companies. Can someone who has experiences with these antibodies recommend the one that you think it is good ? Thank you very much for sharing your experiences! Best regards, /zhe _________________________________________________________________ Add photos to your messages with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=features/featuredemail From histolog <@t> fcv.unl.edu.ar Sun Jul 25 15:22:20 2004 From: histolog <@t> fcv.unl.edu.ar (=?iso-8859-1?Q?Laboratorio_de_Histolog=EDa?=) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Silane from Aldrich In-Reply-To: <002a01c3c996$64fff800$3187080a@wsahs.nsw.gov.au> Message-ID: <006d01c47285$16d39b80$0100a8c0@casa> I need to know if some of you has used Aldrich?s Silane (440140-100ML/3-AMINOPROPYLTRIETHOXYSILANE 99% ). This has a smaller cost, but I don't know their quality. Thank you Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From bills <@t> icpmr.wsahs.nsw.gov.au Sun Jul 25 16:58:12 2004 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Microscope filters In-Reply-To: <2A70D44ECF6F1A4390DD1D98E8BEDEF211138F@wsahs.nsw.gov.au> Message-ID: <002001c47292$7b4f6910$83a7080a@wsahs.nsw.gov.au> Margaret, Many years ago when I did all the photography for the department I used a filter "dydim or diadim" which gave much better results to colour photography using a T64 Kodak Professional colour film. I am not sure if it will be the same for digital cameras, I purchased this filter from a microscope supplier, however I believe that any photographic store should have them. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margaret Blount Sent: Friday, 23 July 2004 5:59 PM To: 'Geoff McAuliffe'; Margaret Blount Cc: Histonet (E-mail) Subject: RE: [Histonet] Microscope filters Dear Geoff, Thanks, that is a great help. My colleague isn't actually using film as he has a digital camera, but I will do a s you suggest and contact the microscope manufacturers for trial samples. I can easily pop into Jessops any time. Thanks again Margaret -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Thursday, July 22, 2004 7:44 PM To: Margaret Blount Cc: Histonet (E-mail) Subject: Re: [Histonet] Microscope filters Hi Margaret: Assuming your colleague is using black and white negative film, a blue filter will pass more blue light to the film and give more density in the negative. When printed, that will translate to the blue subject being lighter on the print. That said, there are lots of blue filters used in photography and some trial and error may be needed. There are lots of different "daylight filters", depending on the light source and the amount of correction needed, an 80A filter might be blue enough for your application. The standard blue filter for making RGB separations is a #47 and it is a fairly dense blue-purple color. A #46 is very similar. Such filters are sold in photo stores that cater to professional photographers (Jessop's in the UK is one) but you might be able to find something in the AudioVisual dept at your institution or perhaps the manufacturer of your microscope might loan you several for a trial, you could then purchase the one that fits your needs. Geoff Margaret Blount wrote: >Hi all, > >I have a chinese colleague who requires a blue filter to suppress staining >for Nissl substance (violet) in brightfield transmitted light microscopy - >does anyone know what filter this is and a supplier of such a filter. So far >I have only identified a Daylight filter, but I am not sure if this would do >the trick or not. If anyone knows anything about this I would be very >grateful as would my colleague. > >Thanks in anticipation. > >Margaret > >Margaret Blount >Chief Technician >Clinical Biochemistry >University of Cambridge >Addenbrooke's Hospital >Hills Road >Cambridge >CB2 2QR > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From JMacDonald <@t> mtsac.edu Sun Jul 25 21:09:54 2004 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Open Position in Southern California In-Reply-To: <16B95C22-DCC6-11D8-AF6A-000A9589219E@mac.com> Message-ID: Diagnostic Immunohistochemistry Laboratory Supervisor Position Description Genzyme Genetics (a division of Genzyme Corporation) is a leader in the application of genetic knowledge in the prevention of disease, improvement of outcomes and reduction of medical costs. We are a leading national provider of Prenatal and Oncology testing as well as Prenatal Genetic Counseling services. As one of the five top biotech companies in the world, we offer an incredible environment in which individuals can excel, building upon their diversified strengths and deliver their personal best. Managing the staff and operations in our Diagnostic Immunohistochemistry (IHC) Laboratory, the successful candidate will be responsible for supervising a laboratory staff of about 15 technicians/technologist responsible for all IHC staining, laboratory operations. This role also requires monitoring areas such as quality control, instrument calibration, and routine laboratory maintenance. Successful candidates must have at least 5+ years of progressive experience working in a clinical or laboratory environment (Histology and/or IHC highly preferred), no less than 2+ years of supervisory/management experience, and a record of achievement working in a clinical laboratory. A bachelor's degree in science/laboratory medicine is required and HT or HTL/ASCP or related certifications are highly desired. This position is for a daytime shift working Mondays through Fridays. If interested please contact: Daisy Jimenez-Joseph Lab Director Genzyme Genetics Los Angeles, CA. Tel: 310-482-5450 Fax:310-482-5778 From JMacDonald <@t> mtsac.edu Sun Jul 25 21:12:40 2004 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Conundrum In-Reply-To: Message-ID: I am curious about the treatment of the slides in formalin. I have heard of a few places that do this but no one seems to know why. Does anyone have a theory on why this helps the tissue stay on the slide? Jennifer MacDonald We use the Ventana XT and place some of our slides in 10% NBF for 30 minutes. This helps the tissue stay on even when the slides haven't been rehydrated. Barb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Sun Jul 25 22:06:54 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Microscope filters Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0635AF6@UTHEVS3.mail.uthouston.edu> Bill and Margaret I think that the filter you are refering to is a didymium filter. If you cannot find one let me know and I probably have one in my lab. This filter accentuates pink to red hues and is very useful for hematoxylin and eosin in which the eosin is a bit faded. If you prepare a digital image however it is possible to made dramatic changes such as color balance, brightness, edge separation etc. with several computer programs such as Adobe, Photoshop etc. Although I am not a photographer, I seem to recall as noted below that for B and W film to supress any specific color you should use a filter of the same color. To accentuate a color use the opposite color e.g. to accentuate red use green to accentuate blue use orange and vice versa. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bill Sinai Sent: Sun 7/25/2004 4:58 PM To: 'Margaret Blount'; histonet (E-mail) Cc: Subject: RE: [Histonet] Microscope filters Margaret, Many years ago when I did all the photography for the department I used a filter "dydim or diadim" which gave much better results to colour photography using a T64 Kodak Professional colour film. I am not sure if it will be the same for digital cameras, I purchased this filter from a microscope supplier, however I believe that any photographic store should have them. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margaret Blount Sent: Friday, 23 July 2004 5:59 PM To: 'Geoff McAuliffe'; Margaret Blount Cc: Histonet (E-mail) Subject: RE: [Histonet] Microscope filters Dear Geoff, Thanks, that is a great help. My colleague isn't actually using film as he has a digital camera, but I will do a s you suggest and contact the microscope manufacturers for trial samples. I can easily pop into Jessops any time. Thanks again Margaret -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Thursday, July 22, 2004 7:44 PM To: Margaret Blount Cc: Histonet (E-mail) Subject: Re: [Histonet] Microscope filters Hi Margaret: Assuming your colleague is using black and white negative film, a blue filter will pass more blue light to the film and give more density in the negative. When printed, that will translate to the blue subject being lighter on the print. That said, there are lots of blue filters used in photography and some trial and error may be needed. There are lots of different "daylight filters", depending on the light source and the amount of correction needed, an 80A filter might be blue enough for your application. The standard blue filter for making RGB separations is a #47 and it is a fairly dense blue-purple color. A #46 is very similar. Such filters are sold in photo stores that cater to professional photographers (Jessop's in the UK is one) but you might be able to find something in the AudioVisual dept at your institution or perhaps the manufacturer of your microscope might loan you several for a trial, you could then purchase the one that fits your needs. Geoff Margaret Blount wrote: >Hi all, > >I have a chinese colleague who requires a blue filter to suppress staining >for Nissl substance (violet) in brightfield transmitted light microscopy - >does anyone know what filter this is and a supplier of such a filter. So far >I have only identified a Daylight filter, but I am not sure if this would do >the trick or not. If anyone knows anything about this I would be very >grateful as would my colleague. > >Thanks in anticipation. > >Margaret > >Margaret Blount >Chief Technician >Clinical Biochemistry >University of Cambridge >Addenbrooke's Hospital >Hills Road >Cambridge >CB2 2QR > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mab70 <@t> medschl.cam.ac.uk Mon Jul 26 03:07:28 2004 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining? Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF2111393@mius2.medlan.cam.ac.uk> Hair fibres tend to be very "sticky"; the follicles, ie, inner and outer root sheaths don't, until you get to the level at which the cells are cornified. Also endogenous biotin will be found in the sebaceous gland in my experience, I never had any problems with it in the follicle itself. All I can suggest is wash well in PBS/TBS and increase your blocking to 10% (or maybe more) normal serum from secondary antibody host prior to the primary. I always used TBS for staining skin and hair follicles and I believe it is supposed to give a cleaner result than PBS. Have you titrated your antibodies sufficiently? If you still get staining in the hair fibre, then I think you may have to live with that, but you shouldn't get background on the follicle cells. I hope this helps, good luck. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Edmondson David (RBV) NHS Christie Tr [mailto:David.Edmondson@christie-tr.nwest.nhs.uk] Sent: Friday, July 23, 2004 2:56 PM To: 'Cao, Xudong' Cc: Histonet (E-mail 2) Subject: RE: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining? Have you tried applying a biotin blocking kit in the procedure? Dave Christie, Manchester -----Original Message----- From: Cao, Xudong [mailto:Xudong_Cao@Brown.edu] Sent: 23 July 2004 14:02 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PGP9.5 staining -- how to get rid of hair follicle background staining? Dear all, I am trying to stain for nerve fibers innervating the skin samples that I have. While I am lucky to get the nerve fiber singnals, I am also getting overwheelingly strong background staining from the hair follicles. I know the follicle stains are background since they are also stained in both of my controls where primary and secondary are omitted are also stained. My question is: how to get rid of the hair follicle stains? any suggestions? I am using Vector's ABC and SG substrate. Thanks Xudong Cao, Ph.D. Biomedical Center Brown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCarpenter764 <@t> aol.com Mon Jul 26 03:31:10 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Practical exam for fall test!!!!! Message-ID: <7C2AE289.77DCF43F.40C6E687@aol.com> I was wondering if anyone has recieved their practical exam for the July 1 deadline.. I haven't received mine yet, and i'm starting to stress...PLEASE HELP Jennell From lpwenk <@t> sbcglobal.net Mon Jul 26 06:42:44 2004 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Practical exam for fall test!!!!! References: <7C2AE289.77DCF43F.40C6E687@aol.com> Message-ID: <001401c47305$b15cdba0$3a2ad445@domainnotset.invalid> I think there was been a change this year. I don't think my students received a "list". They received a letter, notifying them that their application was received and that they met the criteria. And then they were instructed to download the list from the ASCP BOR (Board of Registry) webpage. My students' letter came out about 3 weeks after their deadlines (my HT students applied by Jan. 1, 2004 and they got their letter 3rd week of January. My HTL students applied by April 1, 2004 and received their letter the 3rd week of April. So they aren't on the same cycle as you.). But if you don't receive the letter by the end of July, call 312-738-1336, then press "0" (don't wait for all the options) and ask for the Board of Registry. If you want to download the practical list now, the list for Fall 2004 HT and HTL practicals can be found on the ASCP Board of Registry (BOR) webpage. http://www.ascp.org/bor/certification/index.asp On the right hand side, click on either HT or HTL practical. Practical slides and blocks must be received before Oct. 1, 2004, so you still have a little over 2 months to get your nine slides done (perfectly, of course ;-) ). Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Monday, July 26, 2004 4:31 AM Subject: [Histonet] Practical exam for fall test!!!!! > I was wondering if anyone has recieved their practical exam for the July 1 deadline.. I haven't received mine yet, and i'm starting to stress...PLEASE HELP Jennell > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbecker <@t> pathlabinc.com Mon Jul 26 08:10:11 2004 From: mbecker <@t> pathlabinc.com (Michele Becker) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Practical exam for fall test!!!!! In-Reply-To: <7C2AE289.77DCF43F.40C6E687@aol.com> Message-ID: I recently contacted the ASCP Board of Registry regarding the Fall HT practical. I was told the practical info is released 30 days after the registration deadline (July 1st). However, the BOR is running a little behind schedule so it will probably be later in August before you will see it. Hang in there!! mlb -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JCarpenter764@aol.com Sent: Monday, July 26, 2004 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Practical exam for fall test!!!!! I was wondering if anyone has recieved their practical exam for the July 1 deadline.. I haven't received mine yet, and i'm starting to stress...PLEASE HELP Jennell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Samuel.Jones2 <@t> med.va.gov Mon Jul 26 09:28:51 2004 From: Samuel.Jones2 <@t> med.va.gov (Jones, Samuel) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Job Opening-Dallas Message-ID: <18EE321ED15F364D89A880C2509F2088D98457@VHANTXEXC1> The VA Medical Center, Dallas, is seeking a certified Histology Technician/Technologist (ASCP) to fill a full time position (M/F) within the Histology Section of the Anatomic Pathology Department. The successful applicant must have the ability to produce quality work in routine histology and special stains. The VAMC-Dallas is a full-service facility in the heart of the VA North Texas Health Care System . We are currently located in a new $155 million clinical addition that includes state-of-the-art instrumentation and technology. As part of our team, you will have the opportunity to work with quality, advanced technology and equipment. Interested applicants should address question to Patrick Kunke, Chief Medical Technologist, Monday through Friday, at 214-857-0682. The VAMC-Dallas is an EEO Employer. Samuel E. Jones, MS, HT(ASCP)HTL, QHIC Supervisor Anatomic Pathology VA North Texas Health Care System 4500 South Lancaster Road / 113 Dallas, Texas 75216 Office: 214-857-0659 Fax: 214-302-1457 E-mail: samuel.jones2@med.va.gov From mward <@t> wfubmc.edu Mon Jul 26 10:12:51 2004 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] herpes VIII antibody Message-ID: <61135F0455D33347B5AAE209B903A304076A4EDC@EXCHVS2.medctr.ad.wfubmc.edu> I have had a request for this antibody, herpes VIII. I wondered if anyone can help me with a vendor, conditions, etc.? Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From joseph-galbraith <@t> uiowa.edu Mon Jul 26 10:36:50 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] P504S Message-ID: <5D03ED7B9391D4119D9B0008C76B7B240300860C@uihc-mail1.uihc.uiowa.edu> Richard: We are also working on developing this antibody. We plan on using it in a double stain combo. Good luck. Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Friday, July 23, 2004 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P504S Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GoodwinD <@t> pahosp.com Mon Jul 26 11:34:55 2004 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Hi or moderate complexity Message-ID: <992899E9EC268548AB8DDE246AF8847302B71678@PAHEX01.uphs.upenn.edu> Greetings, All. I'm chagrinned to demonstrate my ignorance, but is histotechnology considered high or moderate complexity testing by CLIA standards? Thanks, Diana Goodwin e-mail: goodwind@pahosp.com From emmie222 <@t> yahoo.com Mon Jul 26 12:46:43 2004 From: emmie222 <@t> yahoo.com (Emily) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers Message-ID: <20040726174643.38022.qmail@web41202.mail.yahoo.com> We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? From JQB7 <@t> CDC.GOV Mon Jul 26 12:58:14 2004 From: JQB7 <@t> CDC.GOV (Bartlett, Jeanine) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers Message-ID: Always a problem. Try the Statmart pens from StatLab. Maybe Leslie will send you a free sample! 1-800-442-3573 X225 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Mon Jul 26 13:04:04 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] re silane from sigma Message-ID: <000e01c4733a$f01baee0$31292bd9@home> Yes....used it for years. Works well, dependant on your protocol, of course --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 From carl.hobbs <@t> kcl.ac.uk Mon Jul 26 13:08:41 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] re microscope filters Message-ID: <001901c4733b$95301ec0$31292bd9@home> You should be able to adjust white balance successfully with a digital camera; there are also the "colour sliders" for fine balance , in any good software.. However, when taking pics of DAB immuno with Hx counterstain I always use an 80A daylight (blue) filter to balance out the yellowish cast of light one gets from a std microscope bulb. Even for my checking microscope, I'll use that type of filter. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 From japoteete <@t> saintfrancis.com Mon Jul 26 13:07:28 2004 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers Message-ID: I think we've been here before, but I wasn't able to pull it out of the archives. Our lab switched to Statmark pens from Statlab (1-800-442-3573), and we have had no problems at all. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa. OK japoteete@saintfrancis.com > -----Original Message----- > From: Emily [SMTP:emmie222@yahoo.com] > Sent: Monday, July 26, 2004 12:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Securline markers > > We have been having a major problem the Securline markers for about a year > - they are pretty much dry when we get them. The company that manufactures > them told me to add xylene to each marker. It works most of the time, but > it takes up a lot of time to add the xylene. My feeling is that I > shouldn't have to do that anyway - they should just make a marker that > actually works. I have tried out many other pens/markers and have found > none to be satisfactory. The ink either fades too much during processing > or just totally washes off. Does anyone have any similar issues? Does > anyone have any ideas or suggestions? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From carl.hobbs <@t> kcl.ac.uk Mon Jul 26 13:13:29 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] re microscope filters Message-ID: <002201c4733c$41097f70$31292bd9@home> Goodness me, Barry.....Didymium filters takes me back to those old days of "fine-tuning" the image to get a quality image of a H&E. Thanks for reminding me. AND the dilemma as to whether to use critical illumination to get "the best" image, as those Photomicrographic books used to recommend, if I recall correctly. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 From JNocito <@t> Pathreflab.com Mon Jul 26 13:22:11 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers In-Reply-To: <20040726174643.38022.qmail@web41202.mail.yahoo.com> Message-ID: Emily, I had several nightmares with those pens. Statlab Medical Products, up by Dallas, 1-800-442-3573 have a new pen that works better. The downfall of these pens is that you have to let the ink dry completely before placing them in solutions. Once the ink is thoroughly dry, we haven't had a problem. The ink is still dark after processing and the pens last about twice as long as the other brand. I think their web page is www.statlab.com Good luck. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sent: Monday, July 26, 2004 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfish <@t> gladstone.ucsf.edu Mon Jul 26 13:39:28 2004 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers In-Reply-To: <20040726174643.38022.qmail@web41202.mail.yahoo.com> References: <20040726174643.38022.qmail@web41202.mail.yahoo.com> Message-ID: Emily, We use RediSharp Plus from Dixon. I order mine from www.tedpella.com. These pens are great and last a long, long time. Available in black and blue. Take care, Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 From convmcm <@t> cc.usu.edu Mon Jul 26 16:50:03 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers In-Reply-To: <20040726174643.38022.qmail@web41202.mail.yahoo.com> Message-ID: <001d01c4735a$81a6a710$4a737b81@Cygnus> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ernestinemiddleton <@t> yahoo.ca Mon Jul 26 20:02:40 2004 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] paraffin blocks Message-ID: <20040727010240.72170.qmail@web51502.mail.yahoo.com> Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals From bill501 <@t> mindspring.com Mon Jul 26 20:51:18 2004 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] paraffin blocks In-Reply-To: <20040727010240.72170.qmail@web51502.mail.yahoo.com> References: <20040727010240.72170.qmail@web51502.mail.yahoo.com> Message-ID: At 9:02 PM -0400 7/26/04, Ernestine Middleton wrote: >Hi; >I like to know how do other Histology Lab. get rid of paraffin >blocks that are 20+years. Do you have a medical waste company >destroy them or do you do it your self? We have a medical waste company destroy them. Bill -- ______________ Bill Blank, MD Heartland Lab, Inc From leclercj <@t> vetanat.unizh.ch Tue Jul 27 04:16:15 2004 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] soften formalin fixed samples Message-ID: Good morning everybody! I have a problem with my formalin fixed samples: they are just too hard. Now I wonder if there is a possibility to soften or "remoisture" them. I would be very pleased if anyone of you felt able to help me. Thank you and best regards, Jocelyne -- VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich From settembr <@t> umdnj.edu Tue Jul 27 06:25:28 2004 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] P504S Message-ID: Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Tue Jul 27 06:28:34 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] AE 13 stain? Message-ID: Does anyone know what AE-13 is? Is it an immunostain, and what is it for? Annette Featherstone HT/MLT -----Original Message----- From: Emily [mailto:emmie222@yahoo.com] Sent: Monday, July 26, 2004 13:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From RBARNHART <@t> summithealth.org Tue Jul 27 06:54:33 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers Message-ID: We have tried many different pens for writing on cassettes and slides, pencils smear for us. The best that we have found is Shur/Mark from Triangle Biomedical Sciences. They have replaceable tips so when they dry up you just put a new tip in. We keep one for writing on cassettes and one for slides (writing on slides makes the pen dull and then hard to write on a cassette). I just received a sample from Pacific Southwest Lab Equipment that so far has done well, PathPen. So far we have used it to write on cassettes and slides and both stayed on. The only thing you MUST let the ink dry, they say for 60 seconds, or it will smear. Becky >>> Emily 07/26/04 01:46PM >>> We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Tue Jul 27 07:01:30 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers Message-ID: We get preprinted xylene resistant labels to use for our paps only. Our liquid base are sent elsewhere for processing and that is what they use, so we started to just for our paps. We get the labels from Surgipath. We told them how we wanted the labels printed (our numbering system, label and ink color, size and our hospital name on them) For our surgical and non-gyn our computer system prints regular labels. Do you have a computer system that you accession the cases in, like Meditech, Sunquest, CoPath etc.? Becky >>> "Connie McManus" 07/26/04 05:50PM >>> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Tue Jul 27 07:08:42 2004 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] AE 13 stain? References: Message-ID: <009c01c473d2$75c88b20$27955c82@patho.unibe.ch> What is probably meant here is a cytokeratin marker which most often is sold as a cocktail of 2 monoclonal antibodies, namely AE1 and AE3. AE1 reacts with an epitope present on cytokeratins 10, 13, 14, 15, 16, 19, AE3 binds to an epitope found on cytokeratins 1, 2, 3, 4, 5, 6, 7, 8. The cocktail is often used as a 'pan-cytokeratin marker' like clone MNF116 or clone Lu-5. Hope this helps Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Featherstone, Annette" To: "'Emily'" ; Sent: Tuesday, July 27, 2004 1:28 PM Subject: [Histonet] AE 13 stain? > Does anyone know what AE-13 is? Is it an immunostain, and what is it for? > Annette Featherstone HT/MLT > From mfredrickson <@t> cohenderm.com Tue Jul 27 07:42:02 2004 From: mfredrickson <@t> cohenderm.com (Michael Fredrickson) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] AE13 Message-ID: <3D55809F621E7C438DA80098CDE3744D0545CA@hub.cohenderm.com> AE 13 is an antibody specific for pilar keratin. Please reference: Arch Dermatol 1990 Feb;126(2):189-94 Mike Fredrickson, Cohen Dermatopathology From Sharon.Davis-Devine <@t> carle.com Tue Jul 27 07:47:09 2004 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Amino Silane coated slides Message-ID: I am looking for anyone who would be willing to share their protocol for coating of Amino Silane slides. Thanks in advance. Sharon Davis-Devine, CT (ASCP) Technical Specialist of Cytology Carle Clinic 602 West University Ave. Urbana, Illinois 61801 Phone: 217-383-3402 sharon.davis-devine@carle.com From convmcm <@t> cc.usu.edu Tue Jul 27 09:34:30 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:47 2005 Subject: [Histonet] Securline markers In-Reply-To: Message-ID: <000001c473e6$d40c3a60$4a737b81@Cygnus> Becky, We don't have any of the software systems you clinical folks use. I just make my labels in MicroSoft Excel -- I took the time to create a template where the cell size is the same dimensions as the frosted end of our slides (this took some trial and error). I formatted one cell with the font size and other attributes we wanted, then copy - pasted a bunch of cells with this formatting. After we embed our blocks, one of us goes to the file, types in the accession numbers, the day's date and then print it on large label paper. It works really well for us except that it IS time consuming. We have found that the Surgipath labels you use don't work well for us. We tried other labels - Nalgene Nunc have some poly paper laser labels that are supposed to be set up in MS Word, but that didn't work for us very well, either. Sometimes the shortest, best, most efficient way is the way you know how to do. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 where am i going and why am i in this handbasket??? -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Tuesday, July 27, 2004 5:02 AM To: convmcm@cc.usu.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Securline markers We get preprinted xylene resistant labels to use for our paps only. Our liquid base are sent elsewhere for processing and that is what they use, so we started to just for our paps. We get the labels from Surgipath. We told them how we wanted the labels printed (our numbering system, label and ink color, size and our hospital name on them) For our surgical and non-gyn our computer system prints regular labels. Do you have a computer system that you accession the cases in, like Meditech, Sunquest, CoPath etc.? Becky >>> "Connie McManus" 07/26/04 05:50PM >>> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From convmcm <@t> cc.usu.edu Tue Jul 27 09:37:15 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] paraffin blocks In-Reply-To: <20040727010240.72170.qmail@web51502.mail.yahoo.com> Message-ID: <000101c473e7$35f9f5f0$4a737b81@Cygnus> We have our own incinerator. That's where all this stuff goes. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Monday, July 26, 2004 6:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin blocks Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jul 27 09:54:50 2004 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin fixed samples Message-ID: Jocelyne You can soften your specimens a bit by soaking them on ice and water. You will loose some of the specimen when you take your first sections. The ice water will swell the tissue a bit. You could also soak the blocks on glycerin, or liquid dish soap. You may what to check your processing schedule of your specimens. If you have too many 100% alcohols this will definitely cause hard tissue. You may be taking bound water out of the tissue. You may also check to see if you have heat on during some of your processing steps. To much heat may also dry your samples depending on the size of the samples. Hope this helps. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Leclerc Jocelyne To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] soften formalin fixed samples western.edu 07/27/2004 04:16 AM Good morning everybody! I have a problem with my formalin fixed samples: they are just too hard. Now I wonder if there is a possibility to soften or "remoisture" them. I would be very pleased if anyone of you felt able to help me. Thank you and best regards, Jocelyne -- VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From gcallis <@t> montana.edu Tue Jul 27 10:13:27 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin fixed samples In-Reply-To: References: Message-ID: <6.0.0.22.0.20040727091008.01b12bf0@gemini.msu.montana.edu> First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Terry.Marshall <@t> rothgen.nhs.uk Tue Jul 27 10:26:22 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin fixed samples Message-ID: Gayle, We are having trouble at the moment with poor clearing, giving a grey cast, and sometimes a steely opaque look to things. Do you think that actually reducing processing times in alcohol and xylene may help, or is this as absurd as it sounds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 27 July 2004 16:13 To: Leclerc Jocelyne; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] soften formalin fixed samples First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BWinters <@t> NCH.ORG Tue Jul 27 10:56:18 2004 From: BWinters <@t> NCH.ORG (Winters, Bert) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] stain for mast cells Message-ID: <270614B321ACB44D8C1D91F4F921FDC3627776@NCH01EX02.nch.org> We have been using an alcian blue stain at pH 0.4 to demonstrate mast cells. Our pathologist would like to use a giemsa stain for mast cells. If someone knows a good stain for mast cells could you let me know. Either a giemsa stain or any other type would be appreciated. From Stanley.Lupo <@t> gsk.com Tue Jul 27 11:43:12 2004 From: Stanley.Lupo <@t> gsk.com (Stanley.Lupo@gsk.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] I have not received any histonet mail in a while Message-ID: Stanley.Lupo@GSK.Com Safety Assessment Mail Code: UE0461 Phone: 610 270-7340 Fax: 610 270-7202 Please resubscribe me to Histonet. I have stopped receiving Histonet email. Thanks. Stan From settembr <@t> umdnj.edu Tue Jul 27 11:54:53 2004 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] AE 13 stain? Message-ID: Emily, I first thought that you meant cytokeratin AE1 and AE3, a commonly used cytokeratin antibody cocktial. Just as Andi Kappeler mentioned. But now, Michael Fredrickson has some specific info about pilar cytokeratin. ? Dana >>> "Featherstone, Annette" 7/27/2004 7:28:34 AM >>> Does anyone know what AE-13 is? Is it an immunostain, and what is it for? Annette Featherstone HT/MLT -----Original Message----- From: Emily [mailto:emmie222@yahoo.com] Sent: Monday, July 26, 2004 13:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lizchlipala <@t> premierhistology.com Tue Jul 27 11:59:53 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] stain for mast cells In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC3627776@NCH01EX02.nch.org> Message-ID: <000201c473fb$2682ac20$74d48a80@LIZ> Bert Toluidine Blue will stain mast cells nicely. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Winters, Bert Sent: Tuesday, July 27, 2004 8:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stain for mast cells We have been using an alcian blue stain at pH 0.4 to demonstrate mast cells. Our pathologist would like to use a giemsa stain for mast cells. If someone knows a good stain for mast cells could you let me know. Either a giemsa stain or any other type would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jengirl1014 <@t> yahoo.com Tue Jul 27 12:14:42 2004 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Securline pens Message-ID: <20040727171442.38455.qmail@web60602.mail.yahoo.com> I use Pigma Micron archival pens available at your local arts and crafts store in the scrapbook section. You have to let them dry, but once the ink is on, it doesn't come off! I use it for slide staining and tissue processing. I don't do as much tissue as a histology lab, so I can prepare for the next day of tisse processing by marking all of my cassettes ahead of time. For more info, feel free to write to me! Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - 100MB free storage! From Jason.Wiese <@t> med.va.gov Tue Jul 27 11:18:35 2004 From: Jason.Wiese <@t> med.va.gov (Jason.Wiese@med.va.gov) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Securline markers Message-ID: The last time I tried to order Secureline II markers from my supplier, I was told they were recalled because there were so many problems with them. I have gone to Statmark pens from Stat Lab (Cat# SMP-BK). As someone said in a previous RE to this subject... as long as you let the ink dry before you throw the cassettes in solution, the Statmark pens rock! ;) Jason Jason Wiese, HT(ASCP) VHAROS Pathology/Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 -----Original Message----- From: Connie McManus [mailto:convmcm@cc.usu.edu] Sent: Monday, July 26, 2004 2:50 PM To: 'Emily'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Securline markers I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lfaucette <@t> niaid.nih.gov Tue Jul 27 12:19:16 2004 From: lfaucette <@t> niaid.nih.gov (Faucette, Lawrence (NIH/NIAID)) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Securline pens Message-ID: <78F91CA4B8F3504E8FE5F13AAF122ED804A02C7A@nihexchange27.nih.gov> #2 pencil anyone ? rarely washes off, Never dries out.......... Wink. Just a thought Lawrence J Faucette Contractor HT ASCP http://www.niaid.nih.gov/dir/services/animalcare/VetPathology/VetPathology-i ndex.html Infectious Disease Pathogenesis Section Comparative Medicine Branch Division of Intramural Research, NIAID, NIH Twinbrook III, Room 2W-01A, MSC 8135 12735 Twinbrook Parkway Bethesda, MD 20892-8135 Telephone 301-451-1056 Fax 301-480-2343 SoBran, Inc. Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. The National Institute of Allergy and Infectious Diseases (NIAID) shall not accept liability for any statement made that are the sender's own and not expressly made on behalf of the NIAID by one of its representatives. -----Original Message----- From: Jennifer Sipes [mailto:jengirl1014@yahoo.com] Sent: Tuesday, July 27, 2004 1:15 PM To: histonet@lists.utsouthwestern.edu; emmie222@yahoo.com Subject: Re: [Histonet] Securline pens I use Pigma Micron archival pens available at your local arts and crafts store in the scrapbook section. You have to let them dry, but once the ink is on, it doesn't come off! I use it for slide staining and tissue processing. I don't do as much tissue as a histology lab, so I can prepare for the next day of tisse processing by marking all of my cassettes ahead of time. For more info, feel free to write to me! Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - 100MB free storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mark.lewis <@t> thermo.com Tue Jul 27 12:24:20 2004 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin fixed samples Message-ID: Terry, Here are some things to perhaps look at. Check on how frequently the waxes have been changed. ( may not have really good clean wax to infiltrate) Is there by any chance water in the waxes or in the Xylenes. (the presence of water in either of these two areas can cause this ) Also check on the frequency of the Xylene changes. ( may not have really good clean Xylene to adequately clear out the Alcohol) Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com "Marshall Terry Dr, Consultant Histopathologist" To: "Gayle Callis" , "Leclerc Jocelyne" , > cc: Sent by: Subject: RE: [Histonet] soften formalin fixed samples histonet-bounces@lists.utsouth western.edu 07/27/2004 11:26 AM Gayle, We are having trouble at the moment with poor clearing, giving a grey cast, and sometimes a steely opaque look to things. Do you think that actually reducing processing times in alcohol and xylene may help, or is this as absurd as it sounds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 27 July 2004 16:13 To: Leclerc Jocelyne; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] soften formalin fixed samples First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jul 27 12:28:22 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] stain for mast cells In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC3627776@NCH01EX02.nch.org> References: <270614B321ACB44D8C1D91F4F921FDC3627776@NCH01EX02.nch.org> Message-ID: <6.0.0.22.0.20040727112449.01aec7d8@gemini.msu.montana.edu> The best toluidine blue mast cell (for connective tissue mast cells) for us has been Churukians method. It leaves no ugly background sometimes due to over-staining with T blue, mast cells are distinct from other cells, clean and very easy to set up. We prefer it to Giemsa staining. Toluidine Blue for Mast Cells, Churukian and Shenk 1. Hydrate formalin fixed tissue sections. Use skin for positive mast cell staining. 2. 0.5% potassium permanganate 2 min 3. Distilled water X 2, 10 dips ea 4. 2% potassium metabisulfite 1 min 5. Tap water, running 3 min 6. Distilled water, 10 dips, 2 changes ea 7. Toluidine blue solution 5 min 0.02% toluidine blue in 0.25% acetic acid (stable for 4 months) 8. Distilled water X 3 9. Dehydrate in 95%, 100% 2 changes each, clear and mount Chururkian and Schenk, J Histotechnology 4(2):85-86, 1981 Toluidine blue method for demonstrating mast cells At 09:56 AM 7/27/2004, you wrote: >We have been using an alcian blue stain at pH 0.4 to demonstrate mast >cells. Our pathologist would like to use a giemsa stain for mast cells. If >someone knows a good stain for mast cells could you let me know. Either a >giemsa stain or any other type would be appreciated. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Julie.Sanders <@t> med.va.gov Tue Jul 27 12:31:10 2004 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] controls for immunos and special stains Message-ID: <457381D92B01BD44B21CF37CC02EBDFD28E8DD@vhacinexc2.v10.med.va.gov> I am having a "discussion" with our lab chief concerning the use of controls with special stain and immunos. He insists that I run a negative control with special stains and a "known negative" control for immunos. Currently we run only a positive control for specials and a postive control and negative patient for immunos. Thoughts and/or protocols would be more than welcome! Thanks, Julie Julie Sanders Supervisor, Anatomic Pathology VAMC, Cincinnati, Ohio -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, July 27, 2004 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 8, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Securline markers (Emily) 2. RE: Securline markers (Bartlett, Jeanine) 3. re silane from sigma (Carl) 4. re microscope filters (Carl) 5. RE: Securline markers (Poteete, Jacquie A.) 6. re microscope filters (Carl) 7. RE: Securline markers (Joe Nocito) 8. Re: Securline markers (Jo Dee Fish) 9. RE: Securline markers (Connie McManus) 10. paraffin blocks (Ernestine Middleton) 11. Re: paraffin blocks (Bill Blank) 12. soften formalin fixed samples (Leclerc Jocelyne) 13. Re: P504S (Dana Settembre) 14. AE 13 stain? (Featherstone, Annette) 15. Re: Securline markers (Rebecca Barnhart) 16. RE: Securline markers (Rebecca Barnhart) 17. Re: AE 13 stain? (Andi Kappeler) 18. AE13 (Michael Fredrickson) 19. Amino Silane coated slides (Sharon.Davis-Devine) 20. RE: Securline markers (Connie McManus) 21. RE: paraffin blocks (Connie McManus) 22. Re: soften formalin fixed samples (mari.ann.mailhiot@leica-microsystems.com) 23. Re: soften formalin fixed samples (Gayle Callis) 24. RE: soften formalin fixed samples (Marshall Terry Dr, Consultant Histopathologist) 25. stain for mast cells (Winters, Bert) 26. I have not received any histonet mail in a while (Stanley.Lupo@gsk.com) 27. Re: AE 13 stain? (Dana Settembre) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Jul 2004 10:46:43 -0700 (PDT) From: Emily Subject: [Histonet] Securline markers To: histonet@lists.utsouthwestern.edu Message-ID: <20040726174643.38022.qmail@web41202.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? ------------------------------ Message: 2 Date: Mon, 26 Jul 2004 13:58:14 -0400 From: "Bartlett, Jeanine" Subject: RE: [Histonet] Securline markers To: "Emily" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Always a problem. Try the Statmart pens from StatLab. Maybe Leslie will send you a free sample! 1-800-442-3573 X225 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 26 Jul 2004 19:04:04 +0100 From: "Carl" Subject: [Histonet] re silane from sigma To: Message-ID: <000e01c4733a$f01baee0$31292bd9@home> Content-Type: text/plain; charset="iso-8859-1" Yes....used it for years. Works well, dependant on your protocol, of course --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 ------------------------------ Message: 4 Date: Mon, 26 Jul 2004 19:08:41 +0100 From: "Carl" Subject: [Histonet] re microscope filters To: Message-ID: <001901c4733b$95301ec0$31292bd9@home> Content-Type: text/plain; charset="iso-8859-1" You should be able to adjust white balance successfully with a digital camera; there are also the "colour sliders" for fine balance , in any good software.. However, when taking pics of DAB immuno with Hx counterstain I always use an 80A daylight (blue) filter to balance out the yellowish cast of light one gets from a std microscope bulb. Even for my checking microscope, I'll use that type of filter. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 ------------------------------ Message: 5 Date: Mon, 26 Jul 2004 13:07:28 -0500 From: "Poteete, Jacquie A." Subject: RE: [Histonet] Securline markers To: 'Emily' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain I think we've been here before, but I wasn't able to pull it out of the archives. Our lab switched to Statmark pens from Statlab (1-800-442-3573), and we have had no problems at all. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa. OK japoteete@saintfrancis.com > -----Original Message----- > From: Emily [SMTP:emmie222@yahoo.com] > Sent: Monday, July 26, 2004 12:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Securline markers > > We have been having a major problem the Securline markers for about a year > - they are pretty much dry when we get them. The company that manufactures > them told me to add xylene to each marker. It works most of the time, but > it takes up a lot of time to add the xylene. My feeling is that I > shouldn't have to do that anyway - they should just make a marker that > actually works. I have tried out many other pens/markers and have found > none to be satisfactory. The ink either fades too much during processing > or just totally washes off. Does anyone have any similar issues? Does > anyone have any ideas or suggestions? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp ------------------------------ Message: 6 Date: Mon, 26 Jul 2004 19:13:29 +0100 From: "Carl" Subject: [Histonet] re microscope filters To: Message-ID: <002201c4733c$41097f70$31292bd9@home> Content-Type: text/plain; charset="iso-8859-1" Goodness me, Barry.....Didymium filters takes me back to those old days of "fine-tuning" the image to get a quality image of a H&E. Thanks for reminding me. AND the dilemma as to whether to use critical illumination to get "the best" image, as those Photomicrographic books used to recommend, if I recall correctly. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 ------------------------------ Message: 7 Date: Mon, 26 Jul 2004 13:22:11 -0500 From: "Joe Nocito" Subject: RE: [Histonet] Securline markers To: "Emily" , Message-ID: Content-Type: text/plain; charset="us-ascii" Emily, I had several nightmares with those pens. Statlab Medical Products, up by Dallas, 1-800-442-3573 have a new pen that works better. The downfall of these pens is that you have to let the ink dry completely before placing them in solutions. Once the ink is thoroughly dry, we haven't had a problem. The ink is still dark after processing and the pens last about twice as long as the other brand. I think their web page is www.statlab.com Good luck. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sent: Monday, July 26, 2004 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 26 Jul 2004 11:39:28 -0700 From: Jo Dee Fish Subject: Re: [Histonet] Securline markers To: Emily Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Emily, We use RediSharp Plus from Dixon. I order mine from www.tedpella.com. These pens are great and last a long, long time. Available in black and blue. Take care, Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 ------------------------------ Message: 9 Date: Mon, 26 Jul 2004 15:50:03 -0600 From: "Connie McManus" Subject: RE: [Histonet] Securline markers To: "'Emily'" , Message-ID: <001d01c4735a$81a6a710$4a737b81@Cygnus> Content-Type: text/plain; charset="us-ascii" I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 26 Jul 2004 21:02:40 -0400 (EDT) From: Ernestine Middleton Subject: [Histonet] paraffin blocks To: histonet@pathology.swmed.edu Message-ID: <20040727010240.72170.qmail@web51502.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals ------------------------------ Message: 11 Date: Mon, 26 Jul 2004 20:51:18 -0500 From: Bill Blank Subject: Re: [Histonet] paraffin blocks To: Ernestine Middleton , histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" At 9:02 PM -0400 7/26/04, Ernestine Middleton wrote: >Hi; >I like to know how do other Histology Lab. get rid of paraffin >blocks that are 20+years. Do you have a medical waste company >destroy them or do you do it your self? We have a medical waste company destroy them. Bill -- ______________ Bill Blank, MD Heartland Lab, Inc ------------------------------ Message: 12 Date: Tue, 27 Jul 2004 11:16:15 +0200 From: Leclerc Jocelyne Subject: [Histonet] soften formalin fixed samples To: Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Good morning everybody! I have a problem with my formalin fixed samples: they are just too hard. Now I wonder if there is a possibility to soften or "remoisture" them. I would be very pleased if anyone of you felt able to help me. Thank you and best regards, Jocelyne -- VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich ------------------------------ Message: 13 Date: Tue, 27 Jul 2004 07:25:28 -0400 From: Dana Settembre Subject: Re: [Histonet] P504S To: Rcartun@harthosp.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 27 Jul 2004 07:28:34 -0400 From: "Featherstone, Annette" Subject: [Histonet] AE 13 stain? To: 'Emily' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone know what AE-13 is? Is it an immunostain, and what is it for? Annette Featherstone HT/MLT -----Original Message----- From: Emily [mailto:emmie222@yahoo.com] Sent: Monday, July 26, 2004 13:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. ------------------------------ Message: 15 Date: Tue, 27 Jul 2004 07:54:33 -0400 From: "Rebecca Barnhart" Subject: Re: [Histonet] Securline markers To: , Message-ID: Content-Type: text/plain; charset=US-ASCII We have tried many different pens for writing on cassettes and slides, pencils smear for us. The best that we have found is Shur/Mark from Triangle Biomedical Sciences. They have replaceable tips so when they dry up you just put a new tip in. We keep one for writing on cassettes and one for slides (writing on slides makes the pen dull and then hard to write on a cassette). I just received a sample from Pacific Southwest Lab Equipment that so far has done well, PathPen. So far we have used it to write on cassettes and slides and both stayed on. The only thing you MUST let the ink dry, they say for 60 seconds, or it will smear. Becky >>> Emily 07/26/04 01:46PM >>> We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 27 Jul 2004 08:01:30 -0400 From: "Rebecca Barnhart" Subject: RE: [Histonet] Securline markers To: , Message-ID: Content-Type: text/plain; charset=US-ASCII We get preprinted xylene resistant labels to use for our paps only. Our liquid base are sent elsewhere for processing and that is what they use, so we started to just for our paps. We get the labels from Surgipath. We told them how we wanted the labels printed (our numbering system, label and ink color, size and our hospital name on them) For our surgical and non-gyn our computer system prints regular labels. Do you have a computer system that you accession the cases in, like Meditech, Sunquest, CoPath etc.? Becky >>> "Connie McManus" 07/26/04 05:50PM >>> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 27 Jul 2004 14:08:42 +0200 From: "Andi Kappeler" Subject: Re: [Histonet] AE 13 stain? To: "Histonet" Message-ID: <009c01c473d2$75c88b20$27955c82@patho.unibe.ch> Content-Type: text/plain; charset="iso-8859-1" What is probably meant here is a cytokeratin marker which most often is sold as a cocktail of 2 monoclonal antibodies, namely AE1 and AE3. AE1 reacts with an epitope present on cytokeratins 10, 13, 14, 15, 16, 19, AE3 binds to an epitope found on cytokeratins 1, 2, 3, 4, 5, 6, 7, 8. The cocktail is often used as a 'pan-cytokeratin marker' like clone MNF116 or clone Lu-5. Hope this helps Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Featherstone, Annette" To: "'Emily'" ; Sent: Tuesday, July 27, 2004 1:28 PM Subject: [Histonet] AE 13 stain? > Does anyone know what AE-13 is? Is it an immunostain, and what is it for? > Annette Featherstone HT/MLT > ------------------------------ Message: 18 Date: Tue, 27 Jul 2004 08:42:02 -0400 From: "Michael Fredrickson" Subject: [Histonet] AE13 To: Message-ID: <3D55809F621E7C438DA80098CDE3744D0545CA@hub.cohenderm.com> Content-Type: text/plain; charset="iso-8859-1" AE 13 is an antibody specific for pilar keratin. Please reference: Arch Dermatol 1990 Feb;126(2):189-94 Mike Fredrickson, Cohen Dermatopathology ------------------------------ Message: 19 Date: Tue, 27 Jul 2004 07:47:09 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] Amino Silane coated slides To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain I am looking for anyone who would be willing to share their protocol for coating of Amino Silane slides. Thanks in advance. Sharon Davis-Devine, CT (ASCP) Technical Specialist of Cytology Carle Clinic 602 West University Ave. Urbana, Illinois 61801 Phone: 217-383-3402 sharon.davis-devine@carle.com ------------------------------ Message: 20 Date: Tue, 27 Jul 2004 08:34:30 -0600 From: "Connie McManus" Subject: RE: [Histonet] Securline markers To: "'Rebecca Barnhart'" , Message-ID: <000001c473e6$d40c3a60$4a737b81@Cygnus> Content-Type: text/plain; charset="us-ascii" Becky, We don't have any of the software systems you clinical folks use. I just make my labels in MicroSoft Excel -- I took the time to create a template where the cell size is the same dimensions as the frosted end of our slides (this took some trial and error). I formatted one cell with the font size and other attributes we wanted, then copy - pasted a bunch of cells with this formatting. After we embed our blocks, one of us goes to the file, types in the accession numbers, the day's date and then print it on large label paper. It works really well for us except that it IS time consuming. We have found that the Surgipath labels you use don't work well for us. We tried other labels - Nalgene Nunc have some poly paper laser labels that are supposed to be set up in MS Word, but that didn't work for us very well, either. Sometimes the shortest, best, most efficient way is the way you know how to do. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 where am i going and why am i in this handbasket??? -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Tuesday, July 27, 2004 5:02 AM To: convmcm@cc.usu.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Securline markers We get preprinted xylene resistant labels to use for our paps only. Our liquid base are sent elsewhere for processing and that is what they use, so we started to just for our paps. We get the labels from Surgipath. We told them how we wanted the labels printed (our numbering system, label and ink color, size and our hospital name on them) For our surgical and non-gyn our computer system prints regular labels. Do you have a computer system that you accession the cases in, like Meditech, Sunquest, CoPath etc.? Becky >>> "Connie McManus" 07/26/04 05:50PM >>> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 27 Jul 2004 08:37:15 -0600 From: "Connie McManus" Subject: RE: [Histonet] paraffin blocks To: "'Ernestine Middleton'" , Message-ID: <000101c473e7$35f9f5f0$4a737b81@Cygnus> Content-Type: text/plain; charset="us-ascii" We have our own incinerator. That's where all this stuff goes. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Monday, July 26, 2004 6:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin blocks Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 27 Jul 2004 09:54:50 -0500 From: mari.ann.mailhiot@leica-microsystems.com Subject: Re: [Histonet] soften formalin fixed samples To: Leclerc Jocelyne Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Jocelyne You can soften your specimens a bit by soaking them on ice and water. You will loose some of the specimen when you take your first sections. The ice water will swell the tissue a bit. You could also soak the blocks on glycerin, or liquid dish soap. You may what to check your processing schedule of your specimens. If you have too many 100% alcohols this will definitely cause hard tissue. You may be taking bound water out of the tissue. You may also check to see if you have heat on during some of your processing steps. To much heat may also dry your samples depending on the size of the samples. Hope this helps. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Leclerc Jocelyne To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] soften formalin fixed samples western.edu 07/27/2004 04:16 AM Good morning everybody! I have a problem with my formalin fixed samples: they are just too hard. Now I wonder if there is a possibility to soften or "remoisture" them. I would be very pleased if anyone of you felt able to help me. Thank you and best regards, Jocelyne -- VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 23 Date: Tue, 27 Jul 2004 09:13:27 -0600 From: Gayle Callis Subject: Re: [Histonet] soften formalin fixed samples To: Leclerc Jocelyne , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.0.20040727091008.01b12bf0@gemini.msu.montana.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 24 Date: Tue, 27 Jul 2004 16:26:22 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] soften formalin fixed samples To: "Gayle Callis" , "Leclerc Jocelyne" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Gayle, We are having trouble at the moment with poor clearing, giving a grey cast, and sometimes a steely opaque look to things. Do you think that actually reducing processing times in alcohol and xylene may help, or is this as absurd as it sounds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 27 July 2004 16:13 To: Leclerc Jocelyne; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] soften formalin fixed samples First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Tue, 27 Jul 2004 10:56:18 -0500 From: "Winters, Bert" Subject: [Histonet] stain for mast cells To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC3627776@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" We have been using an alcian blue stain at pH 0.4 to demonstrate mast cells. Our pathologist would like to use a giemsa stain for mast cells. If someone knows a good stain for mast cells could you let me know. Either a giemsa stain or any other type would be appreciated. ------------------------------ Message: 26 Date: Tue, 27 Jul 2004 12:43:12 -0400 From: Stanley.Lupo@gsk.com Subject: [Histonet] I have not received any histonet mail in a while To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Stanley.Lupo@GSK.Com Safety Assessment Mail Code: UE0461 Phone: 610 270-7340 Fax: 610 270-7202 Please resubscribe me to Histonet. I have stopped receiving Histonet email. Thanks. Stan ------------------------------ Message: 27 Date: Tue, 27 Jul 2004 12:54:53 -0400 From: Dana Settembre Subject: Re: [Histonet] AE 13 stain? To: AFeatherstone@KaleidaHealth.Org, histonet@lists.utsouthwestern.edu, emmie222@yahoo.com Message-ID: Content-Type: text/plain; charset=US-ASCII Emily, I first thought that you meant cytokeratin AE1 and AE3, a commonly used cytokeratin antibody cocktial. Just as Andi Kappeler mentioned. But now, Michael Fredrickson has some specific info about pilar cytokeratin. ? Dana >>> "Featherstone, Annette" 7/27/2004 7:28:34 AM >>> Does anyone know what AE-13 is? Is it an immunostain, and what is it for? Annette Featherstone HT/MLT -----Original Message----- From: Emily [mailto:emmie222@yahoo.com] Sent: Monday, July 26, 2004 13:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 39 *************************************** From Jackie.O'Connor <@t> abbott.com Tue Jul 27 12:55:43 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Carstairs Stain Message-ID: Would someone please send me an INTACT method for Carstairs stain for fibrin and platelets? I found an archived Histonet method from 2001, but there is a step missing. Thanks - I'm half way through the stain already - - - - -read ahead Jack, read ahead..... Thanks! From JCarpenter764 <@t> aol.com Tue Jul 27 13:09:36 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] practical exam clarification? Message-ID: Can someone please help with this one? My supervisor told me that when collecting my tissue I was suppose to cut it by myself... Now, i understand that i have to cut the tissue after processing it and embedding it, but she told me i had to cut it from the initial tissue source like our pathologists and our PA'S do. For example, if i needed a piece of uterus, after the case is signed out, i had to cut sections from that uterus. Can someone help i'm sooooo confused and i want to make sure i'm doing the right thing. Thanks Jennell From mkundu <@t> purdue.edu Tue Jul 27 13:34:40 2004 From: mkundu <@t> purdue.edu (mkundu@purdue.edu) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] NFkappa B References: <20040727171442.38455.qmail@web60602.mail.yahoo.com> Message-ID: <006901c47408$60eea730$5296d280@agriculture.purdue.edu> Hello all, Does anyone know of an antibody for NFkappa B that cross reacts in Rat or mouse. I have someone that wants this and the only NF kappaB that I can find is only specific in human. Thank you all for your time MK From giorgia.setti <@t> kcl.ac.uk Tue Jul 27 13:47:51 2004 From: giorgia.setti <@t> kcl.ac.uk (Giorgia Setti) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] (no subject) Message-ID: <1121168384-1320842@pathology.swmed.edu> Giorgia Setti mailto:giorgia.setti@kcl.ac.uk hello histonetters!!! Does anibody know something about MCP-1 expression in the rat spleen....like if MCP-1 is produced under stimulation or is also produced without stimulation? i am looking for a positive control for MCP-1 staining on rat frozen section but it is difficult to find it!! Help!! thank you giorgia From funderwood <@t> mcohio.org Tue Jul 27 13:49:28 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:48 2005 Subject: [BULK] - [Histonet] Securline markers Message-ID: Newcomer Supply carries two pens that work for slide and cassette labelling. The HistoTec Pen and Moist Mark Plus Pen. 1.800.383.7799 or newcomersupply.com Fred >>> Emily 07/26/04 01:46PM >>> We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jul 27 14:12:17 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] pencil lead deposits on top of sections In-Reply-To: <78F91CA4B8F3504E8FE5F13AAF122ED804A02C7A@nihexchange27.nih .gov> References: <78F91CA4B8F3504E8FE5F13AAF122ED804A02C7A@nihexchange27.nih.gov> Message-ID: <6.0.0.22.0.20040727125748.01b4bcf8@gemini.msu.montana.edu> Unfortunately, pencil lead exfoliates as pencil lead dust (black deposits) that end up as tiny black blobs on top of stained sections. This happens when people are heavy handed and hold the slide where pencil is used for labeling rather than on edges of slides and contaminate the waterbath. We have also observed pencil lead dust deposits on bottom of first processing station e.g. 70% alcohol. Been there, had it, and prefer to fight with pricey little Secureline markers, even StatLab pens have some issues, like smearing unless you give them a tidge of drying time. We often pre-label slides and cassettes in our insure marker is totally dry with either of these pens. Being in research lab is easier to accomplish this task versus a clinical, hospital lab where slides are marked during microtomy session. We have never had Secureline wash off our plastic cassettes nor slides, with exception of red Secureline - a total disaster during one processing, the red pens were thrown out. Keep hoping someone will invent the ideal pen, until then ---- At 11:19 AM 7/27/2004, you wrote: >#2 pencil anyone ? rarely washes off, Never dries out.......... Wink. >Just a thought Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From funderwood <@t> mcohio.org Tue Jul 27 14:29:06 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:23:48 2005 Subject: [BULK] - [Histonet] pencil lead deposits on top of sections Message-ID: Ah Ha!!! So I'm not crazy. A few years back when I first got my tissue tek coverslipper (using the tape) I noticed that precipitate looking stuff. Eventually I figured out that it was lead dust. I had not heard of anyone else having this problem and figured I was just some kind of uncoordinated. mega digited, klutz. Thank you Gayle. Fred >>> Gayle Callis 07/27/04 03:12PM >>> Unfortunately, pencil lead exfoliates as pencil lead dust (black deposits) that end up as tiny black blobs on top of stained sections. This happens when people are heavy handed and hold the slide where pencil is used for labeling rather than on edges of slides and contaminate the waterbath. We have also observed pencil lead dust deposits on bottom of first processing station e.g. 70% alcohol. Been there, had it, and prefer to fight with pricey little Secureline markers, even StatLab pens have some issues, like smearing unless you give them a tidge of drying time. We often pre-label slides and cassettes in our insure marker is totally dry with either of these pens. Being in research lab is easier to accomplish this task versus a clinical, hospital lab where slides are marked during microtomy session. We have never had Secureline wash off our plastic cassettes nor slides, with exception of red Secureline - a total disaster during one processing, the red pens were thrown out. Keep hoping someone will invent the ideal pen, until then ---- At 11:19 AM 7/27/2004, you wrote: >#2 pencil anyone ? rarely washes off, Never dries out.......... Wink. >Just a thought Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Tue Jul 27 15:05:40 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] practical exam clarification? Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403005B25@uihc-mail1.uihc.uiowa.edu> Jennell: I will not speak to the exact current requirements but, when I did my practical for the HTL, I located my own tissue, cut it from the original stored tissue for a signed out case, processed it, embedded it, cut it, stained it, and labeled the slides completely on my own. Where possible I isolated fresh tissue so I could control fixation as well. No one did any part of the work other than myself. Note that you will be graded on tissue selection (meeting the stated criteria), fixation, processing, embedding, cutting, staining, etc so those steps should be your own work, plus you should want to control all these factors yourself and not leave any part of it up to someone else who may not have as high a standard to meet. However, I must add that I did have the advantage of working in the gross room of a large teaching hospital at the time, so I had access to facilities and tissue sources that many people would not. This definitely made my task easier. I do believe that if you get tissue from another institution that you are supposed to acquire fixed tissue and proceed from there. We have had students from the regional schools who did a practicum at our institution use part of their time with us to find, process and embed tissue here and then take the embedded blocks with them to complete the challenge at their home base. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JCarpenter764@aol.com Sent: Tuesday, July 27, 2004 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] practical exam clarification? Can someone please help with this one? My supervisor told me that when collecting my tissue I was suppose to cut it by myself... Now, i understand that i have to cut the tissue after processing it and embedding it, but she told me i had to cut it from the initial tissue source like our pathologists and our PA'S do. For example, if i needed a piece of uterus, after the case is signed out, i had to cut sections from that uterus. Can someone help i'm sooooo confused and i want to make sure i'm doing the right thing. Thanks Jennell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Tue Jul 27 15:26:01 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] controls for immunos and special stains Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403005B26@uihc-mail1.uihc.uiowa.edu> Julie: You are required by CAP to run negative controls for immunos that are specific for the species of the primary antibody AND for EACH pretreatment utilized. There are even some rumblings about isotype specific controls as well but no specific requirement for that as yet to my knowledge. Naturally you must have a positive control as well. Our positive controls are on the same slide as the patient tissue section. We run positive controls that contain elements that stain and don't stain with the primary antibody. The negative controls are patient tissue sections cut onto separate slides and stained (hopefully negative) with species specific immunoglobulins using each pretreatment method. In a worst case scenario there will be one negative control for each test slide run. Ideally your special stain positive controls should contain elements that stain and elements that do not stain. These should also be 'same slide' controls when possible. The elements of your positive control that are known to NOT stain could act as a negative control. Of course the best negative control, if you can find one, would be the same tissue element (ie the same tumor type for example) where one piece was known to have a positive reaction and another piece was known to be negative. We will often include a normal and abnormal tissue in the on-slide control as well. For example, a piece of normal lymph node and a piece of lymphoma lymph node for a Bcl-2 control. This approach provides a nice contrast when there are elements that stain in normal as well as abnormal tissue. Best wishes, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julie.Sanders@med.va.gov Sent: Tuesday, July 27, 2004 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] controls for immunos and special stains I am having a "discussion" with our lab chief concerning the use of controls with special stain and immunos. He insists that I run a negative control with special stains and a "known negative" control for immunos. Currently we run only a positive control for specials and a postive control and negative patient for immunos. Thoughts and/or protocols would be more than welcome! Thanks, Julie Julie Sanders Supervisor, Anatomic Pathology VAMC, Cincinnati, Ohio -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, July 27, 2004 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 8, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Securline markers (Emily) 2. RE: Securline markers (Bartlett, Jeanine) 3. re silane from sigma (Carl) 4. re microscope filters (Carl) 5. RE: Securline markers (Poteete, Jacquie A.) 6. re microscope filters (Carl) 7. RE: Securline markers (Joe Nocito) 8. Re: Securline markers (Jo Dee Fish) 9. RE: Securline markers (Connie McManus) 10. paraffin blocks (Ernestine Middleton) 11. Re: paraffin blocks (Bill Blank) 12. soften formalin fixed samples (Leclerc Jocelyne) 13. Re: P504S (Dana Settembre) 14. AE 13 stain? (Featherstone, Annette) 15. Re: Securline markers (Rebecca Barnhart) 16. RE: Securline markers (Rebecca Barnhart) 17. Re: AE 13 stain? (Andi Kappeler) 18. AE13 (Michael Fredrickson) 19. Amino Silane coated slides (Sharon.Davis-Devine) 20. RE: Securline markers (Connie McManus) 21. RE: paraffin blocks (Connie McManus) 22. Re: soften formalin fixed samples (mari.ann.mailhiot@leica-microsystems.com) 23. Re: soften formalin fixed samples (Gayle Callis) 24. RE: soften formalin fixed samples (Marshall Terry Dr, Consultant Histopathologist) 25. stain for mast cells (Winters, Bert) 26. I have not received any histonet mail in a while (Stanley.Lupo@gsk.com) 27. Re: AE 13 stain? (Dana Settembre) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Jul 2004 10:46:43 -0700 (PDT) From: Emily Subject: [Histonet] Securline markers To: histonet@lists.utsouthwestern.edu Message-ID: <20040726174643.38022.qmail@web41202.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? ------------------------------ Message: 2 Date: Mon, 26 Jul 2004 13:58:14 -0400 From: "Bartlett, Jeanine" Subject: RE: [Histonet] Securline markers To: "Emily" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Always a problem. Try the Statmart pens from StatLab. Maybe Leslie will send you a free sample! 1-800-442-3573 X225 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 26 Jul 2004 19:04:04 +0100 From: "Carl" Subject: [Histonet] re silane from sigma To: Message-ID: <000e01c4733a$f01baee0$31292bd9@home> Content-Type: text/plain; charset="iso-8859-1" Yes....used it for years. Works well, dependant on your protocol, of course --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 ------------------------------ Message: 4 Date: Mon, 26 Jul 2004 19:08:41 +0100 From: "Carl" Subject: [Histonet] re microscope filters To: Message-ID: <001901c4733b$95301ec0$31292bd9@home> Content-Type: text/plain; charset="iso-8859-1" You should be able to adjust white balance successfully with a digital camera; there are also the "colour sliders" for fine balance , in any good software.. However, when taking pics of DAB immuno with Hx counterstain I always use an 80A daylight (blue) filter to balance out the yellowish cast of light one gets from a std microscope bulb. Even for my checking microscope, I'll use that type of filter. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 ------------------------------ Message: 5 Date: Mon, 26 Jul 2004 13:07:28 -0500 From: "Poteete, Jacquie A." Subject: RE: [Histonet] Securline markers To: 'Emily' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain I think we've been here before, but I wasn't able to pull it out of the archives. Our lab switched to Statmark pens from Statlab (1-800-442-3573), and we have had no problems at all. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa. OK japoteete@saintfrancis.com > -----Original Message----- > From: Emily [SMTP:emmie222@yahoo.com] > Sent: Monday, July 26, 2004 12:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Securline markers > > We have been having a major problem the Securline markers for about a year > - they are pretty much dry when we get them. The company that manufactures > them told me to add xylene to each marker. It works most of the time, but > it takes up a lot of time to add the xylene. My feeling is that I > shouldn't have to do that anyway - they should just make a marker that > actually works. I have tried out many other pens/markers and have found > none to be satisfactory. The ink either fades too much during processing > or just totally washes off. Does anyone have any similar issues? Does > anyone have any ideas or suggestions? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp ------------------------------ Message: 6 Date: Mon, 26 Jul 2004 19:13:29 +0100 From: "Carl" Subject: [Histonet] re microscope filters To: Message-ID: <002201c4733c$41097f70$31292bd9@home> Content-Type: text/plain; charset="iso-8859-1" Goodness me, Barry.....Didymium filters takes me back to those old days of "fine-tuning" the image to get a quality image of a H&E. Thanks for reminding me. AND the dilemma as to whether to use critical illumination to get "the best" image, as those Photomicrographic books used to recommend, if I recall correctly. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 ------------------------------ Message: 7 Date: Mon, 26 Jul 2004 13:22:11 -0500 From: "Joe Nocito" Subject: RE: [Histonet] Securline markers To: "Emily" , Message-ID: Content-Type: text/plain; charset="us-ascii" Emily, I had several nightmares with those pens. Statlab Medical Products, up by Dallas, 1-800-442-3573 have a new pen that works better. The downfall of these pens is that you have to let the ink dry completely before placing them in solutions. Once the ink is thoroughly dry, we haven't had a problem. The ink is still dark after processing and the pens last about twice as long as the other brand. I think their web page is www.statlab.com Good luck. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sent: Monday, July 26, 2004 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 26 Jul 2004 11:39:28 -0700 From: Jo Dee Fish Subject: Re: [Histonet] Securline markers To: Emily Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Emily, We use RediSharp Plus from Dixon. I order mine from www.tedpella.com. These pens are great and last a long, long time. Available in black and blue. Take care, Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 ------------------------------ Message: 9 Date: Mon, 26 Jul 2004 15:50:03 -0600 From: "Connie McManus" Subject: RE: [Histonet] Securline markers To: "'Emily'" , Message-ID: <001d01c4735a$81a6a710$4a737b81@Cygnus> Content-Type: text/plain; charset="us-ascii" I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 26 Jul 2004 21:02:40 -0400 (EDT) From: Ernestine Middleton Subject: [Histonet] paraffin blocks To: histonet@pathology.swmed.edu Message-ID: <20040727010240.72170.qmail@web51502.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals ------------------------------ Message: 11 Date: Mon, 26 Jul 2004 20:51:18 -0500 From: Bill Blank Subject: Re: [Histonet] paraffin blocks To: Ernestine Middleton , histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" At 9:02 PM -0400 7/26/04, Ernestine Middleton wrote: >Hi; >I like to know how do other Histology Lab. get rid of paraffin >blocks that are 20+years. Do you have a medical waste company >destroy them or do you do it your self? We have a medical waste company destroy them. Bill -- ______________ Bill Blank, MD Heartland Lab, Inc ------------------------------ Message: 12 Date: Tue, 27 Jul 2004 11:16:15 +0200 From: Leclerc Jocelyne Subject: [Histonet] soften formalin fixed samples To: Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Good morning everybody! I have a problem with my formalin fixed samples: they are just too hard. Now I wonder if there is a possibility to soften or "remoisture" them. I would be very pleased if anyone of you felt able to help me. Thank you and best regards, Jocelyne -- VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich ------------------------------ Message: 13 Date: Tue, 27 Jul 2004 07:25:28 -0400 From: Dana Settembre Subject: Re: [Histonet] P504S To: Rcartun@harthosp.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 27 Jul 2004 07:28:34 -0400 From: "Featherstone, Annette" Subject: [Histonet] AE 13 stain? To: 'Emily' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone know what AE-13 is? Is it an immunostain, and what is it for? Annette Featherstone HT/MLT -----Original Message----- From: Emily [mailto:emmie222@yahoo.com] Sent: Monday, July 26, 2004 13:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. ------------------------------ Message: 15 Date: Tue, 27 Jul 2004 07:54:33 -0400 From: "Rebecca Barnhart" Subject: Re: [Histonet] Securline markers To: , Message-ID: Content-Type: text/plain; charset=US-ASCII We have tried many different pens for writing on cassettes and slides, pencils smear for us. The best that we have found is Shur/Mark from Triangle Biomedical Sciences. They have replaceable tips so when they dry up you just put a new tip in. We keep one for writing on cassettes and one for slides (writing on slides makes the pen dull and then hard to write on a cassette). I just received a sample from Pacific Southwest Lab Equipment that so far has done well, PathPen. So far we have used it to write on cassettes and slides and both stayed on. The only thing you MUST let the ink dry, they say for 60 seconds, or it will smear. Becky >>> Emily 07/26/04 01:46PM >>> We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 27 Jul 2004 08:01:30 -0400 From: "Rebecca Barnhart" Subject: RE: [Histonet] Securline markers To: , Message-ID: Content-Type: text/plain; charset=US-ASCII We get preprinted xylene resistant labels to use for our paps only. Our liquid base are sent elsewhere for processing and that is what they use, so we started to just for our paps. We get the labels from Surgipath. We told them how we wanted the labels printed (our numbering system, label and ink color, size and our hospital name on them) For our surgical and non-gyn our computer system prints regular labels. Do you have a computer system that you accession the cases in, like Meditech, Sunquest, CoPath etc.? Becky >>> "Connie McManus" 07/26/04 05:50PM >>> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 27 Jul 2004 14:08:42 +0200 From: "Andi Kappeler" Subject: Re: [Histonet] AE 13 stain? To: "Histonet" Message-ID: <009c01c473d2$75c88b20$27955c82@patho.unibe.ch> Content-Type: text/plain; charset="iso-8859-1" What is probably meant here is a cytokeratin marker which most often is sold as a cocktail of 2 monoclonal antibodies, namely AE1 and AE3. AE1 reacts with an epitope present on cytokeratins 10, 13, 14, 15, 16, 19, AE3 binds to an epitope found on cytokeratins 1, 2, 3, 4, 5, 6, 7, 8. The cocktail is often used as a 'pan-cytokeratin marker' like clone MNF116 or clone Lu-5. Hope this helps Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Featherstone, Annette" To: "'Emily'" ; Sent: Tuesday, July 27, 2004 1:28 PM Subject: [Histonet] AE 13 stain? > Does anyone know what AE-13 is? Is it an immunostain, and what is it for? > Annette Featherstone HT/MLT > ------------------------------ Message: 18 Date: Tue, 27 Jul 2004 08:42:02 -0400 From: "Michael Fredrickson" Subject: [Histonet] AE13 To: Message-ID: <3D55809F621E7C438DA80098CDE3744D0545CA@hub.cohenderm.com> Content-Type: text/plain; charset="iso-8859-1" AE 13 is an antibody specific for pilar keratin. Please reference: Arch Dermatol 1990 Feb;126(2):189-94 Mike Fredrickson, Cohen Dermatopathology ------------------------------ Message: 19 Date: Tue, 27 Jul 2004 07:47:09 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] Amino Silane coated slides To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain I am looking for anyone who would be willing to share their protocol for coating of Amino Silane slides. Thanks in advance. Sharon Davis-Devine, CT (ASCP) Technical Specialist of Cytology Carle Clinic 602 West University Ave. Urbana, Illinois 61801 Phone: 217-383-3402 sharon.davis-devine@carle.com ------------------------------ Message: 20 Date: Tue, 27 Jul 2004 08:34:30 -0600 From: "Connie McManus" Subject: RE: [Histonet] Securline markers To: "'Rebecca Barnhart'" , Message-ID: <000001c473e6$d40c3a60$4a737b81@Cygnus> Content-Type: text/plain; charset="us-ascii" Becky, We don't have any of the software systems you clinical folks use. I just make my labels in MicroSoft Excel -- I took the time to create a template where the cell size is the same dimensions as the frosted end of our slides (this took some trial and error). I formatted one cell with the font size and other attributes we wanted, then copy - pasted a bunch of cells with this formatting. After we embed our blocks, one of us goes to the file, types in the accession numbers, the day's date and then print it on large label paper. It works really well for us except that it IS time consuming. We have found that the Surgipath labels you use don't work well for us. We tried other labels - Nalgene Nunc have some poly paper laser labels that are supposed to be set up in MS Word, but that didn't work for us very well, either. Sometimes the shortest, best, most efficient way is the way you know how to do. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 where am i going and why am i in this handbasket??? -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Tuesday, July 27, 2004 5:02 AM To: convmcm@cc.usu.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Securline markers We get preprinted xylene resistant labels to use for our paps only. Our liquid base are sent elsewhere for processing and that is what they use, so we started to just for our paps. We get the labels from Surgipath. We told them how we wanted the labels printed (our numbering system, label and ink color, size and our hospital name on them) For our surgical and non-gyn our computer system prints regular labels. Do you have a computer system that you accession the cases in, like Meditech, Sunquest, CoPath etc.? Becky >>> "Connie McManus" 07/26/04 05:50PM >>> I stopped using secureline markers some time ago because the writing on an entire rack of slides came off completely. I agree with you...you shouldn't have to add xylene to pens. They should have better QC than that. We use a pencil to write the accession number on the slides before we cut, then apply paper sticky labels after cover slipping. The information is set up in MS Excel and printed on large label paper. This is time intensive, but we don't have a slide labeler and I can't think of any other options. If there are any out there, I'm all ears, too *g* Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 27 Jul 2004 08:37:15 -0600 From: "Connie McManus" Subject: RE: [Histonet] paraffin blocks To: "'Ernestine Middleton'" , Message-ID: <000101c473e7$35f9f5f0$4a737b81@Cygnus> Content-Type: text/plain; charset="us-ascii" We have our own incinerator. That's where all this stuff goes. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Monday, July 26, 2004 6:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin blocks Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 27 Jul 2004 09:54:50 -0500 From: mari.ann.mailhiot@leica-microsystems.com Subject: Re: [Histonet] soften formalin fixed samples To: Leclerc Jocelyne Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Jocelyne You can soften your specimens a bit by soaking them on ice and water. You will loose some of the specimen when you take your first sections. The ice water will swell the tissue a bit. You could also soak the blocks on glycerin, or liquid dish soap. You may what to check your processing schedule of your specimens. If you have too many 100% alcohols this will definitely cause hard tissue. You may be taking bound water out of the tissue. You may also check to see if you have heat on during some of your processing steps. To much heat may also dry your samples depending on the size of the samples. Hope this helps. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Leclerc Jocelyne To: Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] soften formalin fixed samples western.edu 07/27/2004 04:16 AM Good morning everybody! I have a problem with my formalin fixed samples: they are just too hard. Now I wonder if there is a possibility to soften or "remoisture" them. I would be very pleased if anyone of you felt able to help me. Thank you and best regards, Jocelyne -- VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 23 Date: Tue, 27 Jul 2004 09:13:27 -0600 From: Gayle Callis Subject: Re: [Histonet] soften formalin fixed samples To: Leclerc Jocelyne , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.0.20040727091008.01b12bf0@gemini.msu.montana.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 24 Date: Tue, 27 Jul 2004 16:26:22 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] soften formalin fixed samples To: "Gayle Callis" , "Leclerc Jocelyne" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Gayle, We are having trouble at the moment with poor clearing, giving a grey cast, and sometimes a steely opaque look to things. Do you think that actually reducing processing times in alcohol and xylene may help, or is this as absurd as it sounds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 27 July 2004 16:13 To: Leclerc Jocelyne; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] soften formalin fixed samples First, check you tissue processing schedule, it may be too long - overexposure to alcohols removes too much bound water (bound to proteins) rather than just free water, over exposure to xylene also hardens tissue, and heat of paraffin is very drying. If you samples are very tiny, you can have shorter processing schedule to prevent drying out of tissue. Second, you can face or trim the block, and place it face down on a block of ice with water on top of ice, or just use ice water. This has been discussed recently on Histonet (at great length) - go to archives and do a search for more hints. At 03:16 AM 7/27/2004, you wrote: >Good morning everybody! > >I have a problem with my formalin fixed samples: they are just too hard. Now >I wonder if there is a possibility to soften or "remoisture" them. >I would be very pleased if anyone of you felt able to help me. > >Thank you and best regards, > >Jocelyne >-- >VetSuisse Fakult?t >Veterin?r-Anatomisches Institut >Winterthurerstr. 260 >8057 Z?rich > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Tue, 27 Jul 2004 10:56:18 -0500 From: "Winters, Bert" Subject: [Histonet] stain for mast cells To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC3627776@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" We have been using an alcian blue stain at pH 0.4 to demonstrate mast cells. Our pathologist would like to use a giemsa stain for mast cells. If someone knows a good stain for mast cells could you let me know. Either a giemsa stain or any other type would be appreciated. ------------------------------ Message: 26 Date: Tue, 27 Jul 2004 12:43:12 -0400 From: Stanley.Lupo@gsk.com Subject: [Histonet] I have not received any histonet mail in a while To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Stanley.Lupo@GSK.Com Safety Assessment Mail Code: UE0461 Phone: 610 270-7340 Fax: 610 270-7202 Please resubscribe me to Histonet. I have stopped receiving Histonet email. Thanks. Stan ------------------------------ Message: 27 Date: Tue, 27 Jul 2004 12:54:53 -0400 From: Dana Settembre Subject: Re: [Histonet] AE 13 stain? To: AFeatherstone@KaleidaHealth.Org, histonet@lists.utsouthwestern.edu, emmie222@yahoo.com Message-ID: Content-Type: text/plain; charset=US-ASCII Emily, I first thought that you meant cytokeratin AE1 and AE3, a commonly used cytokeratin antibody cocktial. Just as Andi Kappeler mentioned. But now, Michael Fredrickson has some specific info about pilar cytokeratin. ? Dana >>> "Featherstone, Annette" 7/27/2004 7:28:34 AM >>> Does anyone know what AE-13 is? Is it an immunostain, and what is it for? Annette Featherstone HT/MLT -----Original Message----- From: Emily [mailto:emmie222@yahoo.com] Sent: Monday, July 26, 2004 13:47 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 8, Issue 39 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Tue Jul 27 15:29:18 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] stain for mast cells Message-ID: <5D03ED7B9391D4119D9B0008C76B7B240300860F@uihc-mail1.uihc.uiowa.edu> Bert: We use both a Giemsa and a Toluidine Blue (latter less often) for mast cells. Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Winters, Bert Sent: Tuesday, July 27, 2004 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stain for mast cells We have been using an alcian blue stain at pH 0.4 to demonstrate mast cells. Our pathologist would like to use a giemsa stain for mast cells. If someone knows a good stain for mast cells could you let me know. Either a giemsa stain or any other type would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Jul 27 15:27:01 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:23:48 2005 Subject: [BULK] - [Histonet] pencil lead deposits on top of sections Message-ID: I think it's actually graphite, you guys, or we'd all have lead poisoning. Were they ever really made from lead? Jackie O' "Fred Underwood" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/27/2004 02:29 PM To: cc: Subject: Re: [BULK] - [Histonet] pencil lead deposits on top of sections Ah Ha!!! So I'm not crazy. A few years back when I first got my tissue tek coverslipper (using the tape) I noticed that precipitate looking stuff. Eventually I figured out that it was lead dust. I had not heard of anyone else having this problem and figured I was just some kind of uncoordinated. mega digited, klutz. Thank you Gayle. Fred >>> Gayle Callis 07/27/04 03:12PM >>> Unfortunately, pencil lead exfoliates as pencil lead dust (black deposits) that end up as tiny black blobs on top of stained sections. This happens when people are heavy handed and hold the slide where pencil is used for labeling rather than on edges of slides and contaminate the waterbath. We have also observed pencil lead dust deposits on bottom of first processing station e.g. 70% alcohol. Been there, had it, and prefer to fight with pricey little Secureline markers, even StatLab pens have some issues, like smearing unless you give them a tidge of drying time. We often pre-label slides and cassettes in our insure marker is totally dry with either of these pens. Being in research lab is easier to accomplish this task versus a clinical, hospital lab where slides are marked during microtomy session. We have never had Secureline wash off our plastic cassettes nor slides, with exception of red Secureline - a total disaster during one processing, the red pens were thrown out. Keep hoping someone will invent the ideal pen, until then ---- At 11:19 AM 7/27/2004, you wrote: >#2 pencil anyone ? rarely washes off, Never dries out.......... Wink. >Just a thought Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kosmicdog <@t> hotmail.com Tue Jul 27 15:30:35 2004 From: kosmicdog <@t> hotmail.com (jason m) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] (no subject) Message-ID: Questions about LCM. I am attempting a unique application of Laser Capture. In order isolate pure ovarian surface epithelium I am taking the ovary directly in the OR and rolling it between two glass slides. The surface epithelium easily detaches and sticks (loosely) to the slide. I then follow this protocol for staining and dehydration. slides are frozen in the OR. 5-10 minutes acetone/EtOH fixation (3:1) 30s dH2O 1s hematoxylin 30s dH2O graded EtOH 1 minute each 5 minutes xylenes airdray proceed with LCM The probem I am having is that the cells sometimes become too firmly attached to the slide and will not under any means come off the slide with the LCM scope. I do not have a lot of material to play with so I would appreciate any help anyone might have. Will a longer xylenes incubation detach the cells or a shorter fixation? I need the cells attached firmly enough that they dont come off in the solutions but not so firmly attached that they wont come off the slide via LCM. Does anyone have experience with this type of thing? Thanks for any help. Jason. _________________________________________________________________ Take charge with a pop-up guard built on patented Microsoft® SmartScreen Technology. http://join.msn.com/?pgmarket=en-ca&page=byoa/prem&xAPID=1994&DI=1034&SU=http://hotmail.com/enca&HL=Market_MSNIS_Taglines Start enjoying all the benefits of MSN® Premium right now and get the first two months FREE*. From joseph-galbraith <@t> uiowa.edu Tue Jul 27 15:32:15 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] controls for immunos and special stains Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008610@uihc-mail1.uihc.uiowa.edu> Julie: You are required by CAP to run negative controls for immunos that are specific for the species of the primary antibody AND for EACH pretreatment utilized. There are even some rumblings about isotype specific controls as well but no specific requirement for that as yet to my knowledge. Naturally you must have a positive control as well. Our positive controls are on the same slide as the patient tissue section. We run positive controls that contain elements that stain and don't stain with the primary antibody. The negative controls are patient tissue sections cut onto separate slides and stained (hopefully negative) with species specific immunoglobulins using each pretreatment method. In a worst case scenario there will be one negative control for each test slide run. Ideally your special stain positive controls should contain elements that stain and elements that do not stain. These should also be 'same slide' controls when possible. The elements of your positive control that are known to NOT stain could act as a negative control. Of course the best negative control, if you can find one, would be the same tissue element (ie the same tumor type for example) where one piece was known to have a positive reaction and another piece was known to be negative. We will often include a normal and abnormal tissue in the on-slide control as well. For example, a piece of normal lymph node and a piece of lymphoma lymph node for a Bcl-2 control. This approach provides a nice contrast when there are elements that stain in normal as well as abnormal tissue. Best wishes, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julie.Sanders@med.va.gov Sent: Tuesday, July 27, 2004 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] controls for immunos and special stains I am having a "discussion" with our lab chief concerning the use of controls with special stain and immunos. He insists that I run a negative control with special stains and a "known negative" control for immunos. Currently we run only a positive control for specials and a postive control and negative patient for immunos. Thoughts and/or protocols would be more than welcome! Thanks, Julie Julie Sanders Supervisor, Anatomic Pathology VAMC, Cincinnati, Ohio From kosmicdog <@t> hotmail.com Tue Jul 27 15:34:23 2004 From: kosmicdog <@t> hotmail.com (jason m) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] LCM of epithelial cells Message-ID: Questions about LCM. I am attempting a unique application of Laser Capture. In order isolate pure ovarian surface epithelium I am taking the ovary directly in the OR and rolling it between two glass slides. The surface epithelium easily detaches and sticks (loosely) to the slide. I then follow this protocol for staining and dehydration. slides are frozen in the OR. 5-10 minutes acetone/EtOH fixation (3:1) 30s dH2O 1s hematoxylin 30s dH2O graded EtOH 1 minute each 5 minutes xylenes airdry proceed with LCM The probem I am having is that the cells sometimes become too firmly attached to the slide and will not under any means come off the slide with the LCM scope. I do not have a lot of material to play with so I would appreciate any help anyone might have. Will a longer xylenes incubation detach the cells or a shorter fixation? I need the cells attached firmly enough that they dont come off in the solutions but not so firmly attached that they wont come off the slide via LCM. Does anyone have experience with this type of thing? Thanks for any help. Jason. _________________________________________________________________ Designer Mail isn't just fun to send, it's fun to receive. Use special stationery, fonts and colors. http://join.msn.com/?pgmarket=en-ca&page=byoa/prem&xAPID=1994&DI=1034&SU=http://hotmail.com/enca&HL=Market_MSNIS_Taglines Start enjoying all the benefits of MSN® Premium right now and get the first two months FREE*. From cquinlan <@t> wlgore.com Tue Jul 27 15:37:42 2004 From: cquinlan <@t> wlgore.com (Christie M Quinlan) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] APES/positively charged slides Message-ID: Hi all, I am having problems with plastic sections (MMA) coming off of APES-subbed slides during deplasticization. I don't want to use gelatin for these as they are for immuno. Has anyone else encountered this problem? If so, what method worked for you? Is there any interference with APES solution and the positive charge on PLUS slides? Thanks in advance for any suggestions. Christie Quinlan W.L. Gore and Assoc. Flagstaff, AZ (928)864-3938 From HornHV <@t> archildrens.org Tue Jul 27 16:10:38 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Securline pens Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6322B90B@EMAIL.archildrens.org> Yep, but smudges and sometimes hard to read Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Faucette, Lawrence (NIH/NIAID) Sent: Tuesday, July 27, 2004 12:19 PM To: 'Jennifer Sipes'; histonet@lists.utsouthwestern.edu; emmie222@yahoo.com Subject: RE: [Histonet] Securline pens #2 pencil anyone ? rarely washes off, Never dries out.......... Wink. Just a thought Lawrence J Faucette Contractor HT ASCP http://www.niaid.nih.gov/dir/services/animalcare/VetPathology/VetPatholo gy-i ndex.html Infectious Disease Pathogenesis Section Comparative Medicine Branch Division of Intramural Research, NIAID, NIH Twinbrook III, Room 2W-01A, MSC 8135 12735 Twinbrook Parkway Bethesda, MD 20892-8135 Telephone 301-451-1056 Fax 301-480-2343 SoBran, Inc. Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. The National Institute of Allergy and Infectious Diseases (NIAID) shall not accept liability for any statement made that are the sender's own and not expressly made on behalf of the NIAID by one of its representatives. -----Original Message----- From: Jennifer Sipes [mailto:jengirl1014@yahoo.com] Sent: Tuesday, July 27, 2004 1:15 PM To: histonet@lists.utsouthwestern.edu; emmie222@yahoo.com Subject: Re: [Histonet] Securline pens I use Pigma Micron archival pens available at your local arts and crafts store in the scrapbook section. You have to let them dry, but once the ink is on, it doesn't come off! I use it for slide staining and tissue processing. I don't do as much tissue as a histology lab, so I can prepare for the next day of tisse processing by marking all of my cassettes ahead of time. For more info, feel free to write to me! Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - 100MB free storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From nena.dimaano <@t> stryker.com Tue Jul 27 15:52:16 2004 From: nena.dimaano <@t> stryker.com (Dimaano, Nena) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] TRAP Staining Message-ID: <20BE9059B6FC2D4B9E8FF2A9E2C4EEBD13F2D2@HOS2KEXCHCL.howost.strykercorp.com> Hello everyone, I wanted to make a quick informal survey to see how many researchers/techs are successful using TRAP (histochemistry) staining on formalin fixed, paraffin embedded sections. I would also like to find out those having problems? thanks for your help, Nena Nena Dimaano, MT/HT(ASCP) Advanced Technology Stryker Orthopaedics 325 Corporate Drive Mahwah, NJ 07430 tel: 201-831-5338 fax: 201-831-6224 email: Nena.Dimaano@stryker.com From Jackie.O'Connor <@t> abbott.com Tue Jul 27 16:44:23 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] TRAP Staining Message-ID: I use the Sigma 387a kit on formalin fixed, formic acid decalcified murine bone with beautiful results. Now, if only someone could tell me how to do the same with alk phos . . . . Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotherapeutics "Dimaano, Nena" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/27/2004 03:52 PM To: cc: Subject: [Histonet] TRAP Staining Hello everyone, I wanted to make a quick informal survey to see how many researchers/techs are successful using TRAP (histochemistry) staining on formalin fixed, paraffin embedded sections. I would also like to find out those having problems? thanks for your help, Nena Nena Dimaano, MT/HT(ASCP) Advanced Technology Stryker Orthopaedics 325 Corporate Drive Mahwah, NJ 07430 tel: 201-831-5338 fax: 201-831-6224 email: Nena.Dimaano@stryker.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Jul 27 17:43:03 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? Message-ID: Hi Dana: This is a question that the CAP needs to re-visit because what "they" are asking us to do is simply not practical in today's laboratory environment. As far as I am concerned, the use of a negative control is grossly overstated. Best case scenario in my lab is we run one negative control and it gets treated with the harshest pretreatment used for that particular case. However, we frequently have cases (mostly consults from other hospitals) where a negative control is not run because there just aren't enough unstained slides available or we are staining an H&E or stained cytology slides. In these situations I look for internal "negative controls" (i.e., cells that should be negative). Since we switched to non-avidin/biotin detection several years ago I hardly ever see "non-specific" staining. I would hope that most CAP inspectors would understand the situation and not site you; I never do. Maybe we should start a petition to get the CAP to re-examine this question? Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 p.s. I hope you don't mind, but I sent my response to Histonet as well since this has been a topic of interest recently. >>> Dana Settembre 07/27/04 03:03PM >>> Dr. Cartun, Can I ask you how you handle your negative controls in reference to CAP requirements? Our inspection is in September and our official revised CAP checklist asks and states the following: Are negative controls used for each antibody species? NOTE: A negative control for each primary antibody species must be used. Alternativly, buffer controls can be used if multiple antibodies for each species are included. The controls used should also control for pre-treatment conditions. I would greatly appreciate it if you would take the time to respond. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> Richard Cartun 7/27/2004 11:23:45 AM >>> Hi Dana: Are you sure that BioCare's AMACR isn't "IVD"? RIchard >>> Dana Settembre 07/27/04 07:25AM >>> Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RFail <@t> Charleston.net Tue Jul 27 18:30:30 2004 From: RFail <@t> Charleston.net (Rena) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] test In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC3627774@NCH01EX02.nch.org> Message-ID: <000701c47431$b5b787e0$b611a6a5@renad4yk9b8abe> From laurie.reilly <@t> jcu.edu.au Tue Jul 27 19:08:39 2004 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Coverslip boxes In-Reply-To: Message-ID: <5.1.0.14.0.20040728100630.00a3cec0@mail.jcu.edu.au> Good Morning All, Does anyone have any good uses for empty coverslip boxes? Regards, Laurie. From leclercj <@t> vetanat.unizh.ch Wed Jul 28 03:18:51 2004 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin fixed samples In-Reply-To: <000b01c473c3$a41c1fa0$30155044@your4di1s53ime> Message-ID: am 27.7.2004 12:22 Uhr schrieb pam marcum unter mucram11@comcast.net: > Jocelyne, > > It would be helpful to know how long the tissues are in formalin? What is > your entire processing schedule and which reagents or alcohols are used? It > may be a combination of things not just formalin. What is the exact formula > or percentage of your formalin solution used in fixation? These pieces of > information would help us help you with your problem. > > Pam Marcum > ----- Original Message ----- Hi everybody. Since the sample is not mine but belongs to one of the preparators from our institute, I had to aks for further information but now I got them. The sample has been fixed in 8% formalin for approximately 2 weeks. There was no other process. Our preparator didn`t use alkohol or other reagents. Now the sample, which is intestine, is too hard to model. So what he wants to do is change the shape of the sample without damaging it. Do you know a way to soften the sample? Thank you very much. Jocelyne Leclerc VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich From JNocito <@t> Pathreflab.com Wed Jul 28 07:22:15 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? In-Reply-To: Message-ID: Dr. Cartun, I present a basic IHC wet workshop at the state and NSH periodically. When I get to the QC section, this is what I tell the attendees. One supervisor during one of the presentations just ripped me apart because some inspector cited her lab for not having the "proper" negative controls. I tried to explain to her that most inspectors understand the costs involved with running a negative control for all different types of pretreatments, etc. I'm sorry she had an inspector that took the checklist verbatim. I think there are numerous questions in the checklist that CAP has to address and reword. This just happens to be one of them. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, July 27, 2004 5:43 PM To: histonet@lists.utsouthwestern.edu; settembr@umdnj.edu Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? Hi Dana: This is a question that the CAP needs to re-visit because what "they" are asking us to do is simply not practical in today's laboratory environment. As far as I am concerned, the use of a negative control is grossly overstated. Best case scenario in my lab is we run one negative control and it gets treated with the harshest pretreatment used for that particular case. However, we frequently have cases (mostly consults from other hospitals) where a negative control is not run because there just aren't enough unstained slides available or we are staining an H&E or stained cytology slides. In these situations I look for internal "negative controls" (i.e., cells that should be negative). Since we switched to non-avidin/biotin detection several years ago I hardly ever see "non-specific" staining. I would hope that most CAP inspectors would understand the situation and not site you; I never do. Maybe we should start a petition to get the CAP to re-examine this question? Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 p.s. I hope you don't mind, but I sent my response to Histonet as well since this has been a topic of interest recently. >>> Dana Settembre 07/27/04 03:03PM >>> Dr. Cartun, Can I ask you how you handle your negative controls in reference to CAP requirements? Our inspection is in September and our official revised CAP checklist asks and states the following: Are negative controls used for each antibody species? NOTE: A negative control for each primary antibody species must be used. Alternativly, buffer controls can be used if multiple antibodies for each species are included. The controls used should also control for pre-treatment conditions. I would greatly appreciate it if you would take the time to respond. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> Richard Cartun 7/27/2004 11:23:45 AM >>> Hi Dana: Are you sure that BioCare's AMACR isn't "IVD"? RIchard >>> Dana Settembre 07/27/04 07:25AM >>> Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wfubmc.edu Wed Jul 28 07:44:12 2004 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] TRAP Staining Message-ID: <9AEEF1FB6254224AA355ED285F8491650999C834@EXCHVS2.medctr.ad.wfubmc.edu> Hi Nena, This method works very well on both plastic and paraffin sections. Hermina Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry For Paraffin: Deparaffinize sections through xylenes and alcohol and bring to running deionized water for 5 minutes. For Plastic: Deplasticize through three 20-minute changes of 2-methoxyethyl acetate, two 5-minute changes of acetone followed by running deionized water for 5 minutes. Make up acetate buffer in the volume needed for the number of slides to be stained. 0.2 M ACETATE BUFFER Distilled water for 50.0 ml for 200.0 ml 0.2 M sodium acetate (FW 82.03) 0.82 gm 3.28 gm 50 mM L(+) tartaric acid (FW 230.1) 0.58 gm 2.32 gm Stir using a magnetic stir bar until dissolved; pH to 5.0 Following the 5-minute wash under running water, incubate the sections in 0.2 M acetate buffer for 20 minutes at room temperature. After this time has elapsed, to this same acetate buffer add: 0.5 mg/ml naphtol AS-MX phosphate 25.0 mg 100.0 mg 1.1 mg/ml fast red TR salt 55.0 mg 220.0 mg Incubate the sections from 1 ? 4 hours at 37?C, monitoring after the first hour, until osteoclasts are bright red. Rinse in distilled water, followed by counterstaining in Mayer?s hematoxylin for 1 ? 5 minutes (depending on the age of the hematoxylin), wash in running water for 5 minutes, air-dry and mount with permount. NOTE: Use 10% EDTA for demineralization for optimal results when doing FFPE tissue. Ref. Erlebacher & Derynck J Cell Biol 132: 195 ? 210, 1996 September 30, 2003 (Revised) Hermina Borgerink Wake Forest University Health Sciences Hermina M. Borgerink, BA, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax (336) 716-1515 e-mail hborgeri@wfubmc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dimaano, Nena Sent: Tuesday, July 27, 2004 4:52 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] TRAP Staining Hello everyone, I wanted to make a quick informal survey to see how many researchers/techs are successful using TRAP (histochemistry) staining on formalin fixed, paraffin embedded sections. I would also like to find out those having problems? thanks for your help, Nena Nena Dimaano, MT/HT(ASCP) Advanced Technology Stryker Orthopaedics 325 Corporate Drive Mahwah, NJ 07430 tel: 201-831-5338 fax: 201-831-6224 email: Nena.Dimaano@stryker.com From GDawson <@t> Milw.Dynacare.com Wed Jul 28 08:20:42 2004 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] RE: Statmark Pens Message-ID: All, Statmark pens are, without a doubt, much better than the secureline slide markers. A company that doesn't test their product before shipping it to their customers deserves to lose those customers. But, just an FYI on the Statmark pens: keep a pencil handy to label any IHC slides going into a high pH citrate buffer for antigen retrieval since the Statmark pens will come off in the hot solution. I have yet to find anything else that will take it off though. Glen Dawson HT & QIHC (ASCP) IHC Coordinator Milwaukee, WI From Charles.Embrey <@t> carle.com Wed Jul 28 08:57:41 2004 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] practical exam clarification? Message-ID: I agree with Joe. When I went through Histo School at the AFIP we had a section on gross tissue identification. Any good histotech should understand the terms associated with tissue anatomy (serosa, mucosa....). Since the tissue must be collected and processed to be a certain size and contain certain elements explained to you in the submission guidelines then I would assume it is up to you to collect the tissue. It's not like you have to gross it in. It just shows that you know how to measure and that you can understand what tissue you are looking at. Charles Embrey PA(AAPA), HT(ASCP) Illinois -----Original Message----- From: Galbraith, Joe [mailto:joseph-galbraith@uiowa.edu] Sent: Tuesday, July 27, 2004 3:06 PM To: JCarpenter764@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] practical exam clarification? Jennell: I will not speak to the exact current requirements but, when I did my practical for the HTL, I located my own tissue, cut it from the original stored tissue for a signed out case, processed it, embedded it, cut it, stained it, and labeled the slides completely on my own. Where possible I isolated fresh tissue so I could control fixation as well. No one did any part of the work other than myself. Note that you will be graded on tissue selection (meeting the stated criteria), fixation, processing, embedding, cutting, staining, etc so those steps should be your own work, plus you should want to control all these factors yourself and not leave any part of it up to someone else who may not have as high a standard to meet. However, I must add that I did have the advantage of working in the gross room of a large teaching hospital at the time, so I had access to facilities and tissue sources that many people would not. This definitely made my task easier. I do believe that if you get tissue from another institution that you are supposed to acquire fixed tissue and proceed from there. We have had students from the regional schools who did a practicum at our institution use part of their time with us to find, process and embed tissue here and then take the embedded blocks with them to complete the challenge at their home base. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JCarpenter764@aol.com Sent: Tuesday, July 27, 2004 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] practical exam clarification? Can someone please help with this one? My supervisor told me that when collecting my tissue I was suppose to cut it by myself... Now, i understand that i have to cut the tissue after processing it and embedding it, but she told me i had to cut it from the initial tissue source like our pathologists and our PA'S do. For example, if i needed a piece of uterus, after the case is signed out, i had to cut sections from that uterus. Can someone help i'm sooooo confused and i want to make sure i'm doing the right thing. Thanks Jennell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Wed Jul 28 09:10:09 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] RE: Statmark Pens In-Reply-To: Message-ID: Glen, There is another company out there that doesn't even comment on customer's complaints. I don't know if you remember, but last year I posted a statement about how I was using a new pen (after testing the free pen on fake blocks) that all the numbers came off the blocks. I contacted the company and no one answered by call. I packaged up the pens, the invoice and a little letter requesting my money back. I never heard from them, except when they send me advertisements, which are 86'd immediately. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, Glen Sent: Wednesday, July 28, 2004 8:21 AM Cc: 'HistoNet Server' Subject: [Histonet] RE: Statmark Pens All, Statmark pens are, without a doubt, much better than the secureline slide markers. A company that doesn't test their product before shipping it to their customers deserves to lose those customers. But, just an FYI on the Statmark pens: keep a pencil handy to label any IHC slides going into a high pH citrate buffer for antigen retrieval since the Statmark pens will come off in the hot solution. I have yet to find anything else that will take it off though. Glen Dawson HT & QIHC (ASCP) IHC Coordinator Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera203 <@t> yahoo.com Wed Jul 28 09:13:45 2004 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Amino Silane coated slides In-Reply-To: Message-ID: <20040728141345.59775.qmail@web40905.mail.yahoo.com> Sharon this is what we use in our lab: 2% 3-Aminopropyltriethoxysilane (Sigma) Cat #: A3648 Comercially purchased Silane 15.0 mls Acetone (reagent grade) 750.0 mls Prepare fresh each use. Submerge slides in solution for 2 minutes, Rinse well in two changes of distilled water, tap excess water off of racks and stand slides with frosted end down at an angle and allow to air dry. Ready to use once dry. reference: Weetal, H.H. Biochem.Biphys. ACTA, 212:1 (1970); Histochemical Journal 1986: 18, 271-276. "Sharon.Davis-Devine" wrote: I am looking for anyone who would be willing to share their protocol for coating of Amino Silane slides. Thanks in advance. Sharon Davis-Devine, CT (ASCP) Technical Specialist of Cytology Carle Clinic 602 West University Ave. Urbana, Illinois 61801 Phone: 217-383-3402 sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP) Michigan State University Department of Physiology Division of Human Pathology College of Human Medicine portera203@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Mail Address AutoComplete - You start. We finish. From portera203 <@t> yahoo.com Wed Jul 28 09:16:32 2004 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] APES/positively charged slides In-Reply-To: Message-ID: <20040728141632.28756.qmail@web40904.mail.yahoo.com> Christie - we dry all of our plastic sections overnight in a 60 C. oven - we have found that this helps retain the sections on the slide through most pretreatments that we use. Hope this idea helps you out. Christie M Quinlan wrote:Hi all, I am having problems with plastic sections (MMA) coming off of APES-subbed slides during deplasticization. I don't want to use gelatin for these as they are for immuno. Has anyone else encountered this problem? If so, what method worked for you? Is there any interference with APES solution and the positive charge on PLUS slides? Thanks in advance for any suggestions. Christie Quinlan W.L. Gore and Assoc. Flagstaff, AZ (928)864-3938 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP) Michigan State University Department of Physiology Division of Human Pathology College of Human Medicine portera203@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Mail Address AutoComplete - You start. We finish. From Barry.R.Rittman <@t> uth.tmc.edu Wed Jul 28 09:17:09 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin fixed samples Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0E2DD79@UTHEVS3.mail.uthouston.edu> Jocelyne I do not know what tissue you are using. It is unusual for the tissue to be hard to the point you are describing with this fixative for this fixation time as most of the hardening usually occurs in the processing. If this were for example a piece of gut that was fixed as is i.e. as its original tube and you would like to change this to a flat sheet....I would suggest that you wash the tissue well in water and then soak in a solution of 10% glycerin. This should soften it sufficiently so that you can manipulate the tissue. To maintain this new shape, pin on a piece of cork, wash to remove the glycerin and then complete the processing. Hope that this helps Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leclerc Jocelyne Sent: Wednesday, July 28, 2004 3:19 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] soften formalin fixed samples am 27.7.2004 12:22 Uhr schrieb pam marcum unter mucram11@comcast.net: > Jocelyne, > > It would be helpful to know how long the tissues are in formalin? What is > your entire processing schedule and which reagents or alcohols are used? It > may be a combination of things not just formalin. What is the exact formula > or percentage of your formalin solution used in fixation? These pieces of > information would help us help you with your problem. > > Pam Marcum > ----- Original Message ----- Hi everybody. Since the sample is not mine but belongs to one of the preparators from our institute, I had to aks for further information but now I got them. The sample has been fixed in 8% formalin for approximately 2 weeks. There was no other process. Our preparator didn`t use alkohol or other reagents. Now the sample, which is intestine, is too hard to model. So what he wants to do is change the shape of the sample without damaging it. Do you know a way to soften the sample? Thank you very much. Jocelyne Leclerc VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwhitesi <@t> adventisthealthcare.com Wed Jul 28 10:07:30 2004 From: mwhitesi <@t> adventisthealthcare.com (Marnie Whiteside) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] histo postion in Columbia SC? Message-ID: Hi, I am an HT certified histology tech moving to the Columbia South Carolina area and was wondering if anyone know of any positions avaliable? Any information would be appreciated. Marnie Whiteside HT Washington Adventist Hospital Takoma Park, Maryland 301-891-5155 From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jul 28 10:20:33 2004 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] silanized slides Message-ID: Fill slide racks with glass slides and soak for 2 minutes in: 490 ml. of Absolute Alcohol containing 10 ml. of 3-aminopropyltriethoxysilane (Sigma#A3648, 100 ml@$15.75) Rinse well in 3 changes of distilled water. Change distilled water after 10 racks. Drain and place slides in oven at 60 degrees C for 30 minutes, or until dry. Store slides in dust free box, as everything will stick to them (we use the boxes the slides come in) ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From lab <@t> mcpathology.com Wed Jul 28 09:29:53 2004 From: lab <@t> mcpathology.com (Lab) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] A1A CONTROL TISSUE Message-ID: <000b01c474af$5939de40$70cfa8c0@LAB> hi, our lab recently began running A1A immuno stain on liver biopsies. our pathologist wants to see a positive liver for A1A defieceincy. Tonsil seems to be the suggested control tissue.If anyone could help us out please let me know.He just wants to see in slide that is adult liver. Thanks, Amy @ mcpathology From froyer <@t> bitstream.net Wed Jul 28 11:01:49 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Test Message-ID: <4107CDED.7090808@bitstream.net> Test From Rcartun <@t> harthosp.org Wed Jul 28 11:11:22 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] A1A CONTROL TISSUE Message-ID: I have control tissue for alpha-1-anti-trypsin deficiency. Are you looking for unstained slides or a paraffin block? Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 >>> "Lab" 07/28/04 10:29AM >>> hi, our lab recently began running A1A immuno stain on liver biopsies. our pathologist wants to see a positive liver for A1A defieceincy. Tonsil seems to be the suggested control tissue.If anyone could help us out please let me know.He just wants to see in slide that is adult liver. Thanks, Amy @ mcpathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TEXGRILLN <@t> aol.com Wed Jul 28 11:27:51 2004 From: TEXGRILLN <@t> aol.com (TEXGRILLN@aol.com) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Immunohistochemistry on MMA embedded bone Message-ID: <8e.10caa6b9.2e392e07@aol.com> Looking for some advice on IHC in methylmethacrylate embedded mouse bone sections. i.e. is antigen retrieval generally needed? Diane Osteoscreen, LTD San Antonio, TX 210-614-0770 ext 224 From gcallis <@t> montana.edu Wed Jul 28 11:43:51 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Coverslip boxes In-Reply-To: <5.1.0.14.0.20040728100630.00a3cec0@mail.jcu.edu.au> References: <5.1.0.14.0.20040728100630.00a3cec0@mail.jcu.edu.au> Message-ID: <6.0.0.22.0.20040728104139.01b56fc8@gemini.msu.montana.edu> Take them home to organize and store variety of wood/metal, etc screws at garage workbench. Button boxes, sewing pins storage. Let grand kids put tiny toys in them for fun and games. Otherwise, they get tossed. At 06:08 PM 7/27/2004, you wrote: >Good Morning All, >Does anyone have any good uses for empty coverslip boxes? > > Regards, Laurie. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From MAUGER <@t> email.chop.edu Wed Jul 28 11:44:32 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] LCM slides Message-ID: To Jason, Are you using coated or charged slides? If so, don't. Get some plain glass slides, and hopefully your tissue will come off easier. Jo >>> "jason m" 07/27/04 04:30PM >>> Questions about LCM. I am attempting a unique application of Laser Capture. In order isolate pure ovarian surface epithelium I am taking the ovary directly in the OR and rolling it between two glass slides. The surface epithelium easily detaches and sticks (loosely) to the slide. I then follow this protocol for staining and dehydration. slides are frozen in the OR. 5-10 minutes acetone/EtOH fixation (3:1) 30s dH2O 1s hematoxylin 30s dH2O graded EtOH 1 minute each 5 minutes xylenes airdray proceed with LCM The probem I am having is that the cells sometimes become too firmly attached to the slide and will not under any means come off the slide with the LCM scope. I do not have a lot of material to play with so I would appreciate any help anyone might have. Will a longer xylenes incubation detach the cells or a shorter fixation? I need the cells attached firmly enough that they dont come off in the solutions but not so firmly attached that they wont come off the slide via LCM. Does anyone have experience with this type of thing? Thanks for any help. Jason. _________________________________________________________________ Take charge with a pop-up guard built on patented Microsoft? SmartScreen Technology. http://join.msn.com/?pgmarket=en-ca&page=byoa/prem&xAPID=1994&DI=1034&SU=http://hotmail.com/enca&HL=Market_MSNIS_Taglines Start enjoying all the benefits of MSN? Premium right now and get the first two months FREE*. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Jul 28 11:51:42 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] RE: Statmark Pens In-Reply-To: References: Message-ID: <6.0.0.22.0.20040728104650.01b60d70@gemini.msu.montana.edu> I think the real problem with Secureline pens is the middleman aka vendor buys up a huge supply, and lets pens sit on shelf too long, what you end up buying is a pen already dried out. If vendors rotate their stocks appropriately, we would have fewer problems. So far we have been lucky, although once pen is opened and used, it goes to pot in a hurry! As for both marking pens you mention here, they all come off in concentrated formic acid during the prion protein immunostaining procedure. Our prion microtomy expert uses pencil to mark her slides. At 07:20 AM 7/28/2004, you wrote: >All, > >Statmark pens are, without a doubt, much better than the secureline slide >markers. A company that doesn't test their product before shipping it to >their customers deserves to lose those customers. But, just an FYI on the >Statmark pens: keep a pencil handy to label any IHC slides going into a high >pH citrate buffer for antigen retrieval since the Statmark pens will come >off in the hot solution. I have yet to find anything else that will take it >off though. > >Glen Dawson HT & QIHC (ASCP) >IHC Coordinator >Milwaukee, WI > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From rschoon <@t> email.unc.edu Wed Jul 28 11:53:38 2004 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Coverslip boxes In-Reply-To: <6.0.0.22.0.20040728104139.01b56fc8@gemini.msu.montana.edu> References: <5.1.0.14.0.20040728100630.00a3cec0@mail.jcu.edu.au> <6.0.0.22.0.20040728104139.01b56fc8@gemini.msu.montana.edu> Message-ID: <4107DA12.2060105@email.unc.edu> They also work great as fly holders for the avid fly fisherman.............. -- Robert Schoonhoven, Laboratory of Molecular Carcinogenesis & Mutagenesis Center for Environmental Health & Susceptibility The University of North Carolina CB 7431 Chapel Hill, NC 27599-7431 919.966.6343 office 919.966.6123 fax Gayle Callis wrote: > Take them home to organize and store variety of wood/metal, etc screws > at garage workbench. Button boxes, sewing pins storage. Let grand > kids put tiny toys in them for fun and games. Otherwise, they get > tossed. > > At 06:08 PM 7/27/2004, you wrote: > > >> Good Morning All, >> Does anyone have any good uses for empty coverslip boxes? >> >> Regards, Laurie. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From waltersk <@t> mail.medicine.uiowa.edu Wed Jul 28 12:31:54 2004 From: waltersk <@t> mail.medicine.uiowa.edu (Walters, Katherine S) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] melanoma tumors and CD markers Message-ID: Histology experts, I want to harvest and cryopreserve melanoma tumors with adjacent skin and/or fat tissue and take cut sections at 8-12 mm thickness. I will then use immunochemical staining to detect surface markers on various immune cells including T-cells, B-cells, NK-cells , macrophages and monocytes. The surface markers targeted include: CD3, CD4, CD8, CD19 and CD20, CD16/32, CD49b, CD64 and CD94. I use a two-step indirect staining method, and my secondary antibodies are conjugated with Texas Red. The melanoma cells express GFP. After literature searches, I discovered a rule of thumb unknown to me, that aldehyde fixation generally inhibits detection of leukocyte surface markers. Other researchers reported good results from antigen retrieval on aldehyde-fixed, paraffin-processed tissue. I hoped for similar results with my frozen tissue. Snap-freeze tissue by placing mold in shallow container of 2-Methyl Butane chilled with liquid nitrogen. The problem I have been experiencing repeatedly is that the melanoma tumor cells do not maintain good morphological structure during freezing and/or sectioning. Because they are fairly large with high cytoplasm volume and create soft, loose tumors in the mice, I believed that the cells possibly rupture during freezing or sectioning. This creates a problem in that the GFP in the cytoplasm is released upon cell degradation, and the tumor cells are no longer distinct from other cell types when viewed by fluorescent microscopy, so staining is messy and not informative. Ultimately, I am seeking the method that will provide the best morphological preservation of the tumor with skin attached, while still allowing reliable detection of immune cell infiltration using anti-CD marker antibodies. If anyone has experience with this methodology or can make recommendations, it would be extremely helpful to me. Thank you for taking the time to read this letter and your assistance is greatly appreciated! Sincerely, Jennifer Springsteen Holden Cancer Research Facility 5210 MERF, University of Iowa Iowa City, IA 52242 319-335-7092 jennifer-springsteen@uiowa.edu From convmcm <@t> cc.usu.edu Wed Jul 28 13:19:11 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Coverslip boxes In-Reply-To: <5.1.0.14.0.20040728100630.00a3cec0@mail.jcu.edu.au> Message-ID: <001501c474cf$619232c0$4a737b81@Cygnus> I use them to collect seeds for my garden. They're great for organizing all those different flower seeds. My grandson likes them to keep his fishing lures in, too. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 email: convmcm@cc.usu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Reilly Sent: Tuesday, July 27, 2004 5:09 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Coverslip boxes Good Morning All, Does anyone have any good uses for empty coverslip boxes? Regards, Laurie. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From min_minlu <@t> hotmail.com Wed Jul 28 14:26:57 2004 From: min_minlu <@t> hotmail.com (Min-Min Lu) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] IHF with blue conjugated secondaries Message-ID: Hi Danielle, I have worked out triple fluorescence labeling on mouse tissue with Blue fluorescence(AMCA Streptavidin SA-5008 Vector) Red and Green fluorescence (Molecular Probes). One of three primary antibodies is made in goat. I stained this Ab first. I used Biotin. Anti-Goat IgG (from horse BA-9500 Vector) as second, then Streptavidin AMCA. After I seen blue positive staining I blocked slides with goat serum then use other two primary Ab (from mouse and rabbit). The send Abs are from goat. I have tried Donkey and chick second. The result is not stable. MinMin Lu Molecular Cardiology Research Center University of Pennsylvania 421 Curie BLVD Philadelphia, PA 19104 215-898-0251 >From: "Danielle Crippen" <dcrippen@buckinstitute.org> >To: <histonet@lists.utsouthwestern.edu> >Subject: [Histonet] IHF with blue conjugated secondaries >Date: Wed, 14 Jul 2004 15:39:30 -0700 > >Dear All, > >I have an investigator who is in need of triple fluorescence labeling on mouse brain tissue. The green fluorophore is injected and the request is that we double label for two additional antigens. > >Our problem is that we cannot find a blue conjugated secondary that works at all. We've purchased AMCA Dn x Rb (Jackson) and 350 Dn x Gt (Molecular Probes) and have tried them at every possible working concentration recommended on the spec sheets. We've included GFAP positive controls with Cy3 secondary (this works beautifully) and we've tried using alternate tissue to eliminate the possibility that it's our particular sample. We've also tried varying the blocking method and several different protocols. > >Considering the confines of our microscope set-up, we must use a blue and a red conjugated secondary. Additionally, one of our primary antibodies is made in goat, so both our secondaries must be made in donkey. > >Any suggestions as to vendors, protocols or particular references from the literature are VERY welcome!!! > >Many many thanks in advance!! > >Danielle Crippen >Morphology Core Manager >Buck Institute for Age Research >8001 Redwood Blvd. >Novato, CA 94945 >415-209-2046 >dcrippen@buckinstitute.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar – get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ From gcallis <@t> montana.edu Wed Jul 28 14:36:24 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Immunohistochemistry on MMA embedded bone In-Reply-To: <8e.10caa6b9.2e392e07@aol.com> References: <8e.10caa6b9.2e392e07@aol.com> Message-ID: <6.0.0.22.0.20040728133220.01ae3d88@gemini.msu.montana.edu> More than likely, yes. Particularly if your antigen is compromised by cross linking with formalin. Neil Hand has a publication in Journal of Histotechnology on doing this. Hopefully, the calcium left in the bone will not interfer with immunoglobulin reaching an antigenic site/epitope. If you are looking for murine CD marker, you may be in trouble. In general, this takes frozen sections for many of the lymphocyte aka CD markers. You did not say what you are going to stain for?? He used pressure cooker and removed ALL methylmethacrylate, you cannot do IHC with MMA plastic still in section. From lpjones <@t> srhs-pa.org Wed Jul 28 15:06:45 2004 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Murphy's Laws of Histotechnology Message-ID: <8E7AD740937B954F947F0DB4467EFEE056E930@mail.srhs-pa.org> We've had this taped on our refrigerator forever - it's a little dated, but thought you all might enjoy. Murphy's Laws of Histotechnology Joan Gibson and Christine Jacobs Fort Myers Community Hospital (Not Dated) 1. Stoney prostate will only be cut on your best knife 2. Any compound ordered as a crystal will always be delivered as a liquid. 3. Unscheduled frozens are always brought to the laboratory at lunch time. 4. "Easy to Pour" Xylene cans aren't. 5. Once a ribbon is finally obtained on hard to cut tissue, it will blow across the room. 6. The chance of getting silver nitrate on your fingers during the day is directly proportional to the importance of your social activities that evening. 7. The probability that a section will wash off the slide is directly proportional to the amount of tissue remaining in the block. 8. The first block cut on a reconditioned knife will have a staple in it. 9. The chance of a power failure during the night is directly proportional to the number of rush specimens in the processor. 10. When timing for a stain is crucial, the timer will not function. 11. The need to filter a solution will always be discovered after the slides have been put into that solution. 12. The pathologist paces the most when the tech is working on a frozen. 13. The amount of anxiety felt while waiting for your registry results is directly proportional to your lack of confidence. 14. The chance that you'll need to use acetone is directly proportional to how recently you've done your nails. 15. Serial sections are requested only after you've forgotten to use your "Cling Free". 16. When a tray of slides is dropped, only the slides with unusual pathology will break. 17. The bulb in the microscope will burn out in the middle of a frozen. 18. If a lab party is planned for the afternoon, there will be double the normal workload for the morning. 19. Your good, dependable knife always gives out on the next to last block of the day. 20. Rush supply orders will always be back ordered. From gentras <@t> vetmed.auburn.edu Wed Jul 28 15:23:00 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] NO CHROMIX Message-ID: <6.0.1.1.0.20040727165102.025ce900@mailhost.vetmed.auburn.edu> Hello, are any of you familiar with Godax Laboratories' NO CHROMIX? It's a metal free substitute for dichromates in sulfuric acid used for acid cleaning glassware. I'm wondering is there ever a point after repeat regeneration that it needs to be discarded? Or can one continue use as long as each addition of NO CHROMIX crystals renders the stale/discolored solution, crystal clear? Just checking to find out if it's already been established or if it's another experiment I need to conduct. Thanks, Atoska Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From terribraud <@t> msn.com Wed Jul 28 16:00:10 2004 From: terribraud <@t> msn.com (TERRI BRAUD) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] RE: Histonet Digest, Vol 8, Issue 42 Message-ID: Try using empty coverslip boxes to store EM blocks, really handy! > 1. Re: Coverslip boxes (Gayle Callis) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leclercj <@t> vetanat.unizh.ch Thu Jul 29 03:20:15 2004 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] soften formalin Message-ID: Thank you very much for your help. Jocelyne Leclerc VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich From convmcm <@t> cc.usu.edu Thu Jul 29 09:17:53 2004 From: convmcm <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Securline pens In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6322B90B@EMAIL.archildrens.org> Message-ID: <000001c47576$dac4fc70$4a737b81@Cygnus> Pencil is what I have always used until about 5 yrs ago when I discovered the secureline pens. These worked pretty well at first, then I started having trouble with them and stopped using them. I went back to pencil to temporarily label my slides (we still use pencil on our cassettes). I find that if I use the hardest leads I can find, there isn't any grit that contaminates my sections or reagents and it doesn't smudge or wipe off. In my experience, #2 leads are the worst for using on slides and cassettes. I use a mechanical pencil with 5mm HB leads. We have no problems with those. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 email: convmcm@cc.usu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, July 27, 2004 2:11 PM To: Faucette, Lawrence (NIH/NIAID); Jennifer Sipes; histonet@lists.utsouthwestern.edu; emmie222@yahoo.com Subject: RE: [Histonet] Securline pens Yep, but smudges and sometimes hard to read Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Faucette, Lawrence (NIH/NIAID) Sent: Tuesday, July 27, 2004 12:19 PM To: 'Jennifer Sipes'; histonet@lists.utsouthwestern.edu; emmie222@yahoo.com Subject: RE: [Histonet] Securline pens #2 pencil anyone ? rarely washes off, Never dries out.......... Wink. Just a thought Lawrence J Faucette Contractor HT ASCP http://www.niaid.nih.gov/dir/services/animalcare/VetPathology/VetPatholo gy-i ndex.html Infectious Disease Pathogenesis Section Comparative Medicine Branch Division of Intramural Research, NIAID, NIH Twinbrook III, Room 2W-01A, MSC 8135 12735 Twinbrook Parkway Bethesda, MD 20892-8135 Telephone 301-451-1056 Fax 301-480-2343 SoBran, Inc. Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. The National Institute of Allergy and Infectious Diseases (NIAID) shall not accept liability for any statement made that are the sender's own and not expressly made on behalf of the NIAID by one of its representatives. -----Original Message----- From: Jennifer Sipes [mailto:jengirl1014@yahoo.com] Sent: Tuesday, July 27, 2004 1:15 PM To: histonet@lists.utsouthwestern.edu; emmie222@yahoo.com Subject: Re: [Histonet] Securline pens I use Pigma Micron archival pens available at your local arts and crafts store in the scrapbook section. You have to let them dry, but once the ink is on, it doesn't come off! I use it for slide staining and tissue processing. I don't do as much tissue as a histology lab, so I can prepare for the next day of tisse processing by marking all of my cassettes ahead of time. For more info, feel free to write to me! Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com --------------------------------- Do you Yahoo!? New and Improved Yahoo! Mail - 100MB free storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Thu Jul 29 09:46:49 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008611@uihc-mail1.uihc.uiowa.edu> Dr. Cartun: I would be first in line to sign that petition. We used to just run a negative for each species (as you say in the harsest conditions utilized) until the latest verbage change. We had changed prior to our last inspection but the inspector did indeed check to see if we were in compliance regarding negative controls for each pretreatment. As I noted this did indeed considerably increase cost without any revenue gain since in the worst case you could have a negative control for each test Ab used. However, it does appear that CAP is rather serious about this and there have even been rumblings about isotype specific neg's which would make the situation even more complex. We too have switched to non-avidin/biotin detection systems and also rarely see any background staining. Sometimes it seems that the vigorous science of research carries into the cost conscious clinical world perhaps more than necessary. However, every time that I get to thinking that neg's always seem to be rather meaningless, then once in a RARE, RARE while we do detect a patient that does have some specific staining on some of the extra negative slides that we run today, so perhaps CAP does have a point. Best wishes, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, July 27, 2004 5:43 PM To: histonet@lists.utsouthwestern.edu; settembr@umdnj.edu Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? Hi Dana: This is a question that the CAP needs to re-visit because what "they" are asking us to do is simply not practical in today's laboratory environment. As far as I am concerned, the use of a negative control is grossly overstated. Best case scenario in my lab is we run one negative control and it gets treated with the harshest pretreatment used for that particular case. However, we frequently have cases (mostly consults from other hospitals) where a negative control is not run because there just aren't enough unstained slides available or we are staining an H&E or stained cytology slides. In these situations I look for internal "negative controls" (i.e., cells that should be negative). Since we switched to non-avidin/biotin detection several years ago I hardly ever see "non-specific" staining. I would hope that most CAP inspectors would understand the situation and not site you; I never do. Maybe we should start a petition to get the CAP to re-examine this question? Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 p.s. I hope you don't mind, but I sent my response to Histonet as well since this has been a topic of interest recently. >>> Dana Settembre 07/27/04 03:03PM >>> Dr. Cartun, Can I ask you how you handle your negative controls in reference to CAP requirements? Our inspection is in September and our official revised CAP checklist asks and states the following: Are negative controls used for each antibody species? NOTE: A negative control for each primary antibody species must be used. Alternativly, buffer controls can be used if multiple antibodies for each species are included. The controls used should also control for pre-treatment conditions. I would greatly appreciate it if you would take the time to respond. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> Richard Cartun 7/27/2004 11:23:45 AM >>> Hi Dana: Are you sure that BioCare's AMACR isn't "IVD"? RIchard >>> Dana Settembre 07/27/04 07:25AM >>> Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jul 29 10:01:05 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] PCR for mycobacterial DNA Message-ID: Is anyone in "Histo" land doing PCR for mycobacterial DNA in paraffin sections (or do you know of anyone doing this test)? We were doing this test here, but our Director of Molecular Pathology left for "greener" pastures and, as a result, we are not offering it at this time. Thank you. Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 From ftulenko06 <@t> jcu.edu Thu Jul 29 10:27:47 2004 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] question Message-ID: Has anybody had any experience using JB-4 plus embedding kits, or can anybody recommend an embedding media (and a place to order it from) used for light microscopy that allows good quality 1-2 micron sections? Thanks, Frank From tflore <@t> lsuhsc.edu Thu Jul 29 10:57:51 2004 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:23:48 2005 Subject: [Histonet] question Message-ID: Frank, your question is too vague. Please be more specific. what do you want to do with the plastic or epoxy? Immunoperoxidase? Special Stains as in paraffin? Just an H&E Stain High Resolution light microscopy only Transmission electron microscopy? How large are the tissues that you will be processing? What ultramicrotomes do you have available? Teresa Flores LSUHSC EM Lab New Orleans, LA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ftulenko06@jcu.edu Sent: Thursday, July 29, 2004 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question Has anybody had any experience using JB-4 plus embedding kits, or can anybody recommend an embedding media (and a place to order it from) used for light microscopy that allows good quality 1-2 micron sections? Thanks, Frank _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Thu Jul 29 11:09:21 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] PCR for mycobacterial DNA Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008612@uihc-mail1.uihc.uiowa.edu> Dr Cartun: We do PCR for M. tub and M av but NOT on paraffin sections. Sorry. Good luck Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Thursday, July 29, 2004 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PCR for mycobacterial DNA Is anyone in "Histo" land doing PCR for mycobacterial DNA in paraffin sections (or do you know of anyone doing this test)? We were doing this test here, but our Director of Molecular Pathology left for "greener" pastures and, as a result, we are not offering it at this time. Thank you. Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Thu Jul 29 11:10:19 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] question Message-ID: Hi Frank, I remember using JB4 , was it Leica or did we buy from LKB at the time or was that "Historesin" It was fairly good up to a point but we gave it up for the getting of good immuno on the samples rather than section thickness. And then Feather blades made 2um sections very possible Neil Hand in Nottingham UK. had a recipe for a HEMA resin that he used to get good immuno but the JB4/Historesin was not co-operative in our hands. If you need that recipe get back to me, or him. David, Christie Hosp' Manchester UK -----Original Message----- From: ftulenko06@jcu.edu [mailto:ftulenko06@jcu.edu] Sent: 29 July 2004 16:28 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question Has anybody had any experience using JB-4 plus embedding kits, or can anybody recommend an embedding media (and a place to order it from) used for light microscopy that allows good quality 1-2 micron sections? Thanks, Frank _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wimer.Helen <@t> NMNH.SI.EDU Thu Jul 29 11:29:30 2004 From: Wimer.Helen <@t> NMNH.SI.EDU (Helen Wimer) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] question Message-ID: I section fish on a regular basis and use the JB4 kit from Polysciences. I have experienced good results at 1-2 microns and stained with a PAS stain using metanil yellow as a counter stain. Feel free to email me any questions. Helen F. Wimer Wimer.Helen@NMNH.SI.EDU Smithsonian Institution MSC NW Washington , DC 20560 (301) 238-3786 From Janice.Fightmaster <@t> HCAhealthcare.com Thu Jul 29 12:02:35 2004 From: Janice.Fightmaster <@t> HCAhealthcare.com (Fightmaster Janice) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] scalpel blades for grossing Message-ID: <1734C20AE655CD449599A15EDC589D89017CA57D@orlex05.hca.corpad.net> I am interested to know what types of safety scalpel blades for pathology grossing that list members are using. Which ones on the market are the best to use? Our pathologists currently use a #22 non-sterile blade with a #six handle. The handle is rather "fat" and they like that. The safety ones that I have info on all seem to have very flat handles and the docs don't like them. Thanks! Janice Fightmaster Pathology Supervisor Oak Hill Hospital 11375 Cortez Blvd. Brooksville, FL 34613 (352)597-6363 Ext. 3636 From Jason.Wiese <@t> med.va.gov Thu Jul 29 12:40:03 2004 From: Jason.Wiese <@t> med.va.gov (Wiese, Jason VHAROS) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] JCAHO questions for histology Message-ID: I run a small VA histo lab in Oregon. I am getting my first JCAHO inspection in two weeks. I have been around for these inspections in the past, but most questions were directed at the lab manager. I am the sole tech in histo, and am wondering if anyone can give me an idea of what questions I can expect. I know they will be doing tracer methodology etc in the main lab, but I am not sure what to expect in histo. I have printed the 2004 JCAHO survey guide and the frequently asked questions, but I find almost nothing that applies to me. Any ideas?? Thank You! Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 From NSEARCY <@t> swmail.sw.org Thu Jul 29 13:16:43 2004 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Antibody Interp help Message-ID: <04Jul29.131654cdt.86617@healthcare.sw.org> Need to find some information on how to interpret Beta-2 Microglobulin. Thanks From GauchV <@t> mail.amc.edu Thu Jul 29 13:47:51 2004 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Asbestos and Silicone Analysis Message-ID: Does anyone know of a laboratory in or near NY state that does analysis of lung tissue to determine the presence of asbestos or silicone? I am having a very hard time locating one and we have a case that we need to send for this testing as soon as possible. Any help would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY From Kim.Kolman <@t> med.va.gov Thu Jul 29 14:24:57 2004 From: Kim.Kolman <@t> med.va.gov (Kim.Kolman@med.va.gov) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] paraffin blocks Message-ID: Did I miss all the replies on this subject? I was looking forward to reading all the reponses; my lab is currently in a dilemma about how to handle disposal of these items. Afraid to share/admit? Kim Kolman, HT (ASCP) VA Eastern Kansas Health Care System Eisenhower VA Medical Center Leavenworth, Ks. -----Original Message----- From: Connie McManus [mailto:convmcm@cc.usu.edu] Sent: Tuesday, July 27, 2004 9:37 AM To: 'Ernestine Middleton'; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin blocks We have our own incinerator. That's where all this stuff goes. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Monday, July 26, 2004 6:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin blocks Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Thu Jul 29 14:24:35 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] paraffin blocks Message-ID: Recycle thru Yankee Candle? Gary Gill -----Original Message----- From: Kim.Kolman@med.va.gov [mailto:Kim.Kolman@med.va.gov] Sent: Thursday, July 29, 2004 2:25 PM To: convmcm@cc.usu.edu; ernestinemiddleton@yahoo.ca; histonet@pathology.swmed.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] paraffin blocks Did I miss all the replies on this subject? I was looking forward to reading all the reponses; my lab is currently in a dilemma about how to handle disposal of these items. Afraid to share/admit? Kim Kolman, HT (ASCP) VA Eastern Kansas Health Care System Eisenhower VA Medical Center Leavenworth, Ks. -----Original Message----- From: Connie McManus [mailto:convmcm@cc.usu.edu] Sent: Tuesday, July 27, 2004 9:37 AM To: 'Ernestine Middleton'; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin blocks We have our own incinerator. That's where all this stuff goes. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Monday, July 26, 2004 6:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin blocks Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Thu Jul 29 14:24:35 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] paraffin blocks Message-ID: Recycle thru Yankee Candle? Gary Gill -----Original Message----- From: Kim.Kolman@med.va.gov [mailto:Kim.Kolman@med.va.gov] Sent: Thursday, July 29, 2004 2:25 PM To: convmcm@cc.usu.edu; ernestinemiddleton@yahoo.ca; histonet@pathology.swmed.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] paraffin blocks Did I miss all the replies on this subject? I was looking forward to reading all the reponses; my lab is currently in a dilemma about how to handle disposal of these items. Afraid to share/admit? Kim Kolman, HT (ASCP) VA Eastern Kansas Health Care System Eisenhower VA Medical Center Leavenworth, Ks. -----Original Message----- From: Connie McManus [mailto:convmcm@cc.usu.edu] Sent: Tuesday, July 27, 2004 9:37 AM To: 'Ernestine Middleton'; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin blocks We have our own incinerator. That's where all this stuff goes. Connie McManus Utah Veterinary Diagnostics Laboratory Utah State University Logan, UT Phone: 435/797-1891 fax: 435/797-2805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Monday, July 26, 2004 6:03 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin blocks Hi; I like to know how do other Histology Lab. get rid of paraffin blocks that are 20+years. Do you have a medical waste company destroy them or do you do it your self? Thank you for answer me as quickly as possible. Ernestine Middleton, Manager Montefiore Med. Ct. Bronx, NY 914-920-4157 --------------------------------- Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Thu Jul 29 14:34:24 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? In-Reply-To: <5D03ED7B9391D4119D9B0008C76B7B2403008611@uihc-mail1.uihc.uiowa.edu> Message-ID: Howdy all, Many moons ago when I was the supervisor of the IHC at the AFIP, I'm talking mid-lat 1980's, we used to run polyclonals using PAP, our negative was a normal rabbit serum, and we used to run our monoclonals ABC with a normal mouse serum negative. At that time we were staining (by hand of course) 350 slides a day. Once when I was QCing the slides, all the polyclonals looked fine, but I noticed that all the monoclonals, including the negative had background staining. Come to find out after a little digging, the specimen came from a person who had been bitten by a rat some time ago. My theory was that mouse and rat are close and maybe the patient had mouse antibodies. That was the only case I can remember that a negative was a good thing. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Galbraith, Joe Sent: Thursday, July 29, 2004 9:47 AM To: Richard Cartun; histonet@lists.utsouthwestern.edu; settembr@umdnj.edu Subject: RE: [Histonet] Re: Dr. Cartun can you answer a CAP question? Dr. Cartun: I would be first in line to sign that petition. We used to just run a negative for each species (as you say in the harsest conditions utilized) until the latest verbage change. We had changed prior to our last inspection but the inspector did indeed check to see if we were in compliance regarding negative controls for each pretreatment. As I noted this did indeed considerably increase cost without any revenue gain since in the worst case you could have a negative control for each test Ab used. However, it does appear that CAP is rather serious about this and there have even been rumblings about isotype specific neg's which would make the situation even more complex. We too have switched to non-avidin/biotin detection systems and also rarely see any background staining. Sometimes it seems that the vigorous science of research carries into the cost conscious clinical world perhaps more than necessary. However, every time that I get to thinking that neg's always seem to be rather meaningless, then once in a RARE, RARE while we do detect a patient that does have some specific staining on some of the extra negative slides that we run today, so perhaps CAP does have a point. Best wishes, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, July 27, 2004 5:43 PM To: histonet@lists.utsouthwestern.edu; settembr@umdnj.edu Subject: [Histonet] Re: Dr. Cartun can you answer a CAP question? Hi Dana: This is a question that the CAP needs to re-visit because what "they" are asking us to do is simply not practical in today's laboratory environment. As far as I am concerned, the use of a negative control is grossly overstated. Best case scenario in my lab is we run one negative control and it gets treated with the harshest pretreatment used for that particular case. However, we frequently have cases (mostly consults from other hospitals) where a negative control is not run because there just aren't enough unstained slides available or we are staining an H&E or stained cytology slides. In these situations I look for internal "negative controls" (i.e., cells that should be negative). Since we switched to non-avidin/biotin detection several years ago I hardly ever see "non-specific" staining. I would hope that most CAP inspectors would understand the situation and not site you; I never do. Maybe we should start a petition to get the CAP to re-examine this question? Richard Richard Cartun, Ph.D. Director, Immunopathology Hartford Hospital Hartford, CT 06102 p.s. I hope you don't mind, but I sent my response to Histonet as well since this has been a topic of interest recently. >>> Dana Settembre 07/27/04 03:03PM >>> Dr. Cartun, Can I ask you how you handle your negative controls in reference to CAP requirements? Our inspection is in September and our official revised CAP checklist asks and states the following: Are negative controls used for each antibody species? NOTE: A negative control for each primary antibody species must be used. Alternativly, buffer controls can be used if multiple antibodies for each species are included. The controls used should also control for pre-treatment conditions. I would greatly appreciate it if you would take the time to respond. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> Richard Cartun 7/27/2004 11:23:45 AM >>> Hi Dana: Are you sure that BioCare's AMACR isn't "IVD"? RIchard >>> Dana Settembre 07/27/04 07:25AM >>> Sorry, Using Biocare Medical's AMACR which is Research Use Only. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 7/23/2004 5:14:14 PM >>> Is anyone using an antibody to "alpha-methylacyl-CoA racemase" (P504S) that is labeled "IVD" or "ASR"? Thank you! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajohnson <@t> aipathology.com Thu Jul 29 16:08:47 2004 From: ajohnson <@t> aipathology.com (Amy Johnson) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] test Message-ID: <016A64931E1ED511B12C0002B30260803086AE@SERV001> havent received any histo net mail lately just checking to see if it is working From jlinda <@t> ces.clemson.edu Thu Jul 29 16:28:11 2004 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] JB-4 kit Message-ID: <5.2.1.1.2.20040729172054.028726e0@mailhost.ces.clemson.edu> Frank, The JB-4 kit is sold in the United States by Polysciences and they have wonderful technical help in the form of "Polysci Pam" (AKA -- Pam Marcum) at pmarcum@polysciences.com. I have also used a kit called Technovit 7100 by EBS Sciences. Both kits work well for 2 micron sections. Hopefully you are not located in the southeastern United States as we are having major problems with rain and humidty....real problems with glycol methacrylate polymerization! Good Luck, Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From maria <@t> ski.org Thu Jul 29 17:28:46 2004 From: maria <@t> ski.org (Maria Mejia) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] looking for nc82 monoclonal antibody!! Message-ID: <41097A1E.1070406@ski.org> Greetings! I'm looking for a vendor OR for anyone who's using the nc82 monoclonal antibody that marks synaptic neuropil, but not the cellular cortex, fiber tracts or glia in insect tissue. I would be most grateful for any information OR for vendor contact regarding this antibody! regards Maria Mejia Smith-Ketterwell Eye Research Institute San Francisco, CA Email: maria@ski.org Phone: 415-345-2185/2165 From ploykasek <@t> phenopath.com Thu Jul 29 18:35:56 2004 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Scheduling Message-ID: Hi all. We are currently looking at some workflow issues, along with scheduling changes. I would like some feedback from anyone using rotating 10 hour shifts. Doesn't sound like fun to me, but from time to time coworkers bring it up. Thanks for the input. Patti Loykasek PhenoPath Laboratories Seattle, WA From histolog <@t> fcv.unl.edu.ar Thu Jul 29 20:54:14 2004 From: histolog <@t> fcv.unl.edu.ar (=?iso-8859-1?Q?Laboratorio_de_Histolog=EDa?=) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Microwave oven In-Reply-To: <002001c47292$7b4f6910$83a7080a@wsahs.nsw.gov.au> Message-ID: <000501c475d8$1e25dc80$0100a8c0@casa> I wanted to know if some of you uses a microwave oven of 1200 Watts to antigen retrieval?? You can send me their protocol?? Thank you. Hugo Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From doscwk <@t> nus.edu.sg Thu Jul 29 21:57:14 2004 From: doscwk <@t> nus.edu.sg (Chan Wai Kam) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] MMA/GMA processing Message-ID: Hi Histonetters, Our lab handles only paraffin specimens. We would like some advice regarding MMA/GMA. If we were to include MMA/GMA specimens, do we require any special equipment or can the processing be done manually? We have a Reichert Jung polycut; can we use this for sectioning both types of specimens? And lastly, how much would it costs in terms of additional equipment if any, that we would need to get? Thanks, Julee Chan Orthopaedic Surgery National University of Singapore From bruyntjes <@t> voeding.tno.nl Fri Jul 30 00:51:46 2004 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] (no subject) Message-ID: <3B070848E7C2204F9DEB8BCFD76772800210D6BC@ntexch1.voeding.tno.nl> Hi all Is anyone familiar with an antibody directed against alfa-2-microglobulin for rat tisuue? J.P. Bruijntjes TNO Nutrition and Food Research Toxicology and Appllied Pharmacology Utrechtse weg 48 PO Box 360 3700 AJ Zeist The Netherlands T +31 30 6944480 F +31 30 6960264 This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From sharon.willman <@t> bms.com Fri Jul 30 09:01:57 2004 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Insitu Hybridization Markers Message-ID: <410A54D5.3090504@bms.com> Hi, I am in need of information and procedures on insitu hybridization markers for proteins in rodent liver. Any tips or information would be most appreciated. Thanks in advance for your help! Sharon Willman From twheelock <@t> mclean.harvard.edu Fri Jul 30 08:48:06 2004 From: twheelock <@t> mclean.harvard.edu (Timothy R. Wheelock) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Rodent Processing Schedule? Message-ID: <410A5196.2070901@mclean.harvard.edu> Hi Everyone: Does anyone have a protocol for processing whole, formalin fixed mouse brains? The mouse brains need to be processed whole. I am concerned that over-processing, using a schedule meant for human brain tissue, will dry the tissue out excessively and make the serial sectioning a nightmare. Also, would the same schedule apply to whole rat brains, or should the times be increased a bit? Thank you. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont MA Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From gcallis <@t> montana.edu Fri Jul 30 10:16:13 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Rodent Processing Schedule? In-Reply-To: <410A5196.2070901@mclean.harvard.edu> References: <410A5196.2070901@mclean.harvard.edu> Message-ID: <6.0.0.22.0.20040730090251.01b36e90@gemini.msu.montana.edu> No, whole rat brains are larger and would require a longer processing schedule. We have processed whole mouse brains using automated VIP (Sakura Finetek) with NO TEMPERATURE added to dehydration stations nor clearing as this will add to tissue drying. Make sure your mouse brains are totally fixed, perfusion is ideal, followed by immersion overnight. We process 70, 80, 95 X 3, 100 X 3, Xylene X 1, Clearite 3 X 1 (or you can use 2 changes of Clearite 3), and 4 changes of paraffin 30 min X 2 changes, 1 hour x 2 changes. All stations are at 1 hour with exception of paraffins, just to shorten total time in that for 3 hours. Use vacuum and pressure for all stations, and do not exceed 60C with paraffin infiltration, avoiding excess heat helps. What you want to avoid is removing the bound water on proteins (this leads to hard, dry tissues) only free water in tissues spaces. Some labs like to have first dehydration stations at lower concentrations of alcohol, 50, 70, 80, 95 X 2, 100 X 2 or 3, clearing, etc. Xylene tends to harden tissue, but Clearite 3 (Richard Allan) and Propar (ANATECH) are more forgiving, single aliphatic hydrocarbon xylene substitutes. These are only guidelines as your conditions may be slightly different. Recommended is do some processing runs on normal brain just to see what is optimal for your fixation and processor conditions. We recently had to customize processing schedules after some test runs for both Periodiate/lysine/paraformaldehyde perfused hamster brain (coronal sections) and tongue, with a separate schedule for mouse coronal brain sections. At 07:48 AM 7/30/2004, you wrote: >Hi Everyone: > >Does anyone have a protocol for processing whole, formalin fixed mouse >brains? The mouse brains need to be processed whole. >I am concerned that over-processing, using a schedule meant for human >brain tissue, will dry the tissue out excessively and make the serial >sectioning a nightmare. >Also, would the same schedule apply to whole rat brains, or should the >times be increased a bit? >Thank you. > >Tim Wheelock >Harvard Brain Tissue Resource Center >McLean Hospital >Belmont MA > > > >Any information, including protected health information (PHI), transmitted >in this email is intended only for the person or entity to which it is >addressed and may contain information that is privileged, confidential and or >exempt from disclosure under applicable Federal or State law. Any review, >retransmission, dissemination or other use of or taking of any action in >reliance upon, protected health information (PHI) by persons or entities other >than the intended recipient is prohibited. If you received this email in >error, >please contact the sender and delete the material from any computer. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From juan.gutierrez <@t> christushealth.org Fri Jul 30 10:31:04 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] scalpel blades for grossing Message-ID: We are keeping the fat ones also, both for the 22's and the 60's. The other day the Fisher rep. came in to show me some new "safety" blades and in the process of the demo he manage to cut MY finger. In all my years of histology using "non-safety" blades I had never cut myself with a scalpel. Go figure. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Fightmaster Janice [mailto:Janice.Fightmaster@HCAhealthcare.com] Sent: Thursday, July 29, 2004 12:03 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] scalpel blades for grossing I am interested to know what types of safety scalpel blades for pathology grossing that list members are using. Which ones on the market are the best to use? Our pathologists currently use a #22 non-sterile blade with a #six handle. The handle is rather "fat" and they like that. The safety ones that I have info on all seem to have very flat handles and the docs don't like them. Thanks! Janice Fightmaster Pathology Supervisor Oak Hill Hospital 11375 Cortez Blvd. Brooksville, FL 34613 (352)597-6363 Ext. 3636 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Fri Jul 30 10:35:36 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] JCAHO questions for histology Message-ID: Are you under CAP? If you are JCAHO will most likely skip the histo lab, at least that has been our experience. If not, take a couple of days off. Just kidding. Since I've always worked in labs that were under CAP I have not had to endure a JCAHO inspection. Good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor Christus Santa Rosa Hospital 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@med.va.gov] Sent: Thursday, July 29, 2004 12:40 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] JCAHO questions for histology I run a small VA histo lab in Oregon. I am getting my first JCAHO inspection in two weeks. I have been around for these inspections in the past, but most questions were directed at the lab manager. I am the sole tech in histo, and am wondering if anyone can give me an idea of what questions I can expect. I know they will be doing tracer methodology etc in the main lab, but I am not sure what to expect in histo. I have printed the 2004 JCAHO survey guide and the frequently asked questions, but I find almost nothing that applies to me. Any ideas?? Thank You! Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Fri Jul 30 10:44:09 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Scheduling Message-ID: I did 10 hour shifts when I did QC at Pepsi and I thought it was great. Three day weekends every week! The extra couple of hours a day are easy to get used to. Good luck. -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Thursday, July 29, 2004 6:36 PM To: histonet Subject: [Histonet] Scheduling Hi all. We are currently looking at some workflow issues, along with scheduling changes. I would like some feedback from anyone using rotating 10 hour shifts. Doesn't sound like fun to me, but from time to time coworkers bring it up. Thanks for the input. Patti Loykasek PhenoPath Laboratories Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chau0056 <@t> umn.edu Fri Jul 30 11:38:12 2004 From: chau0056 <@t> umn.edu (chau0056) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Cryosectioning, fixing and H&E staining Message-ID: <200407301638.i6UGcCuP030521@trojan.software.umn.edu>   Hello, I want to cryosection collagen (bovine type I) matrixes seeded with human stromal fibroblasts. I am looking at collagen production by the fibroblast. My two questions are 1) Is it better to fix before or after cryosectioning? I’m using a 1% glutaraldehyde- 10% formalin fixative. 2) After section I want to use a Hematoxylin (Modified Harris) and Eosin Y alcoholic stain, but I’m having trouble seeing the Eosin stain. I have tried shortening the destaining step, but it doesn’t seem to be working. Also, the staining steps, some of my samples don’t want to stay on the slide. Eric Department of Mechanical Engineering University of Minnesota From joseph-galbraith <@t> uiowa.edu Fri Jul 30 12:01:41 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] JCAHO questions for histology Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403008613@uihc-mail1.uihc.uiowa.edu> Jason: JCAHO inspectors are now using a new tracer technique that allows them to ask any random employee direct questions rather than just the management. Usually these questions relate to your job training and to safety (OSHA type stuff) and security issues (HIPA type stuff). Everyone should be aware that JCAHO is taking a more aggressive approach when it comes to assessing whether front line personnel really do know the rules. Good luck Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wiese, Jason VHAROS Sent: Thursday, July 29, 2004 12:40 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] JCAHO questions for histology I run a small VA histo lab in Oregon. I am getting my first JCAHO inspection in two weeks. I have been around for these inspections in the past, but most questions were directed at the lab manager. I am the sole tech in histo, and am wondering if anyone can give me an idea of what questions I can expect. I know they will be doing tracer methodology etc in the main lab, but I am not sure what to expect in histo. I have printed the 2004 JCAHO survey guide and the frequently asked questions, but I find almost nothing that applies to me. Any ideas?? Thank You! Jason Wiese, HT(ASCP) VHAROS Histology 913 Garden Valley Blvd Roseburg, OR 97470 (541)-440-1000 x44751 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From degaboh <@t> rice.edu Fri Jul 30 12:22:39 2004 From: degaboh <@t> rice.edu (ZP) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] theoretical question on fixing and section (formalin and cryosections) In-Reply-To: <20040730171033.115F2198FB@fungible3.mail.rice.edu> Message-ID: <20040730172239.D96331DB06@handler9.mail.rice.edu> Hello all, This question refers to bone tissue. If you want to do IHC, cryosections generally give better results, right? Does it make sense to fix the tissue in 10% formalin, decalcify (say in formic acid), and then "embed" in OCT and freeze in dry ice to take cryosections? It just seems like people do 10% formalin and follow that with paraffin embedding.. Thanks! Zarana Patel degaboh@rice.edu From JefThompson <@t> salud.unm.edu Fri Jul 30 12:24:54 2004 From: JefThompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] non-fluorescent dual label Message-ID: Hello Histonetters, Does anyone have any good protocols for non-fluorescent dual label immunostaining? We are staining hemmorhaged brain tissue and are have big problems with autofluorescence. I have a poor protocol for DAB and alkaline phosphatase staining and wonder if there is a good one out there or if anyone hasd any good alternatives to the DAB/AP stain. Any help would be greatly appreciated. Thanks, Jeff Thompson From Stacy_McLaughlin <@t> cooley-dickinson.org Fri Jul 30 12:45:31 2004 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] unsubscribe Message-ID: <3D502BBF5356D31184650090275B750D0346C804@MAIL> Stacy McLaughlin HT (ASCP) THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From gcallis <@t> montana.edu Fri Jul 30 12:46:06 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] theoretical question on fixing and section (formalin and cryosections) In-Reply-To: <20040730172239.D96331DB06@handler9.mail.rice.edu> References: <20040730171033.115F2198FB@fungible3.mail.rice.edu> <20040730172239.D96331DB06@handler9.mail.rice.edu> Message-ID: <6.0.0.22.1.20040730113023.01b058a0@gemini.msu.montana.edu> "Right?" Not necessarily. Many people do unfixed bone frozen sections ( not fixed nor decalcified) and sectioned using the Tape Transfer Cryojane system, Instrumedics. Some antigens hold up well with formalin fixation and decalcification followed by paraffin processing. It will depend on what you want to see. It is always advisable to use gentle decalcification, buffered formic acid or EDTA, but there are decalcified bone retrieval kits, Biogenex has one, to help counter the effects of decalcification. Doing retrieval on frozen sections is sometimes difficult with section loss. Remember that if an acid decalcifier can compromises the antigen EDTA is a good choice, followed by paraffin processing. There are excellent review publications on the effects of decalcification on bone immunohistochemistry. This is also true of the effects of fixation on antigens. If you fix, decalcify and want to snap freeze, the acid would have to be rinsed out of bone, and the decalcified bone would need sucrose cryoprotection. There are methods that fix, decalcify with bone snap frozen, for IHC. Go to J Histochemstry and Cytochemistry, look up Kim Kusser as the author. It is not a SHORT method, time consuming if one has to do more rapid diagnositic work. At 11:22 AM 7/30/2004, you wrote: >Hello all, > >This question refers to bone tissue. If you want to do IHC, cryosections >generally give better results, right? Does it make sense to fix the tissue >in 10% formalin, decalcify (say in formic acid), and then "embed" in OCT and >freeze in dry ice to take cryosections? It just seems like people do 10% >formalin and follow that with paraffin embedding.. > >Thanks! > >Zarana Patel >degaboh@rice.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From mpwu <@t> MIT.EDU Fri Jul 30 12:06:20 2004 From: mpwu <@t> MIT.EDU (Melissa P Wu) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] DAB fading/pH range Message-ID: <1091207180.410a800c1498b@webmail.mit.edu> Hi, I know this has been addressed in previous posts but I haven't been able to find an answer to this question specifically. What is the pH range that the DAB stain is stable in? Supporting info: I am using DAB (sigma, tablet form) on Drosophila ovaries (enhanced with Nickel). After about 8 minutes of the reaction, the tissues will stain black. I mount in 70% glycerol/PBS (pH 7.2), and after a day the stain fades or disappears. I also tried 90% glycerol/PBS, but the stain faded here as well. I read somewhere that the nailpolish could affect the staining, so I stored one sample in 70% glycerol/PBS, but the color faded in these samples after a day. I called Sigma and they suggested using a resin mount, but I don't have access to that so I used Vectashield mounting media, the sample's color faded with this one also. Also, a side question. This is my lab's first time using HRP reactions, I tried one on a control sample and let it incubate for about 20 minutes in the DAB reaction. I was surprised to see the tissue stain black. I am pretty sure it is not reacting to endogenous HRP as I used methanol earlier to destroy the activity. I am also pretty sure it is not due to the primary antibody non-specifically binding, as its antigen is BrdU. Is it just background staining that I am seeing, or perhaps my sample was contaminated? Any help with this situation would be appreciated. Thanks, Melissa Wu From gcallis <@t> montana.edu Fri Jul 30 13:09:57 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Cryosectioning, fixing and H&E staining In-Reply-To: <200407301638.i6UGcCuP030521@trojan.software.umn.edu> References: <200407301638.i6UGcCuP030521@trojan.software.umn.edu> Message-ID: <6.0.0.22.1.20040730115645.01ae8360@gemini.msu.montana.edu> We have good succes with fresh tissue frozen sections, cut and immerse immediately into neutral buffered formalin - fix for as long as you want, 20 mintues, or even 10 min. We often leave the FS in for a week on longer! Mount your sections onto Plus Charge slides. Make your running tap water rinses gentle!! Rough handling of FS will knock them off the slide. If you use strong Ammonium hydroxide to blue, the alkaline solution can eat sections off slides. Use Scotts tap water substitute or Richard Allan bluing solution. Destaining after hematoxylin is not going to adjust your eosin staining unless you do not rinse properly after bluing step. Rinse section with tap water, distilled water rinse, Hematoxylin 1 (Richard Allan) for 1 1/2 min - progressive hematoxylin, not Harris variety. Running tap water rinse 1 min Clarifier (Richard Allan) - 10 dips (fast!) Rinse tap water 1 min Bluing solution (Richard Allan) 1 min Rinse tap water for 1 min 70% ethanol 1 min Eosin Y 30 sec to 1 min (Richard Allan product) - normally frozen sections take up eosin readily. 95% X 2, 100X 2, clear and mount. Rinses are 30 dips or more each. Our frozen section H&E staining looks no different than our paraffin sections. Your problem may be the pH of your eosin or adjusting the pH before you go into eosin. The rinse after bluing is critical to remove any remaining cations, and the 70% alcohol rinse helps do that plus equilibrates the section with the same percentage of alcohol contained in eosin y solution. In the past, we observed Gluteraldehyde fixed tissue stains with eosin differently as compared to NBF fixed tissue. At 10:38 AM 7/30/2004, you wrote: > >Hello, > I want to cryosection collagen (bovine type I) matrixes seeded with >human stromal fibroblasts. I am looking at collagen production by the >fibroblast. My two questions are 1) Is it better to fix before or after >cryosectioning? I'm using a 1% glutaraldehyde- 10% formalin fixative. 2) >After section I want to use a Hematoxylin (Modified Harris) and Eosin Y >alcoholic stain, but I'm having trouble seeing the Eosin stain. I have >tried shortening the destaining step, but it doesn't seem to be working. >Also, the staining steps, some of my samples don't want to stay on the >slide. > >Eric >Department of Mechanical Engineering >University of Minnesota > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From carl.hobbs <@t> kcl.ac.uk Fri Jul 30 13:13:34 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] re Rodent Processing Schedule Message-ID: <001301c47660$edeac2e0$7ced9b51@home> I routinely process mouse and rat whole brains ( tho' I will also bisect/cut into appropriate segments too) on my Leica "dip'n'dunk" processor. x1 each of 30/70/90% IMS, x3 100% IMS, x1 100%IMS/Xylene - 1:1, x2 xylenes, x2 wax. Mouse brains tend to go on a schedule of 2hrs in each and every pot altho if I've got whole rat brains to process at the same time I'll add the meeses to a schedule of 4hrs in each pot. Never had any problems. If I'm processing rat/mouse brain slices( sliced after fixation in "10%" formalin, whether perfused or immersion -fixed, ) I'll usually use the 2hr schedule. I also put mouse/rat/fly/fish/chick embryos thro' on same 2hr/pot -schedule. --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.726 / Virus Database: 481 - Release Date: 22/07/2004 From terribraud <@t> msn.com Fri Jul 30 13:19:21 2004 From: terribraud <@t> msn.com (TERRI BRAUD) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] RE: Histonet Digest, Vol 8, Issue 44 Message-ID: We are saving all the pieces parts to build our own pathologist, who we will then chain to the gross bench, Frankenstein style! > 5. RE: paraffin blocks (Kim.Kolman@med.va.gov) From juan.gutierrez <@t> christushealth.org Fri Jul 30 13:59:16 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] RE: Histonet Digest, Vol 8, Issue 44 Message-ID: Make sure you put a brain in this time:<) -----Original Message----- From: TERRI BRAUD [mailto:terribraud@msn.com] Sent: Friday, July 30, 2004 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 8, Issue 44 We are saving all the pieces parts to build our own pathologist, who we will then chain to the gross bench, Frankenstein style! > 5. RE: paraffin blocks (Kim.Kolman@med.va.gov) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Miller <@t> UTSouthwestern.edu Fri Jul 30 14:16:00 2004 From: Jacqueline.Miller <@t> UTSouthwestern.edu (Jacqueline Miller) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] transferring paraffin ribbon Message-ID: Hi everyone, I would like to know about the techniques that histotechs use to cut paraffin sections--especially how the ribbon is transferred from microtome to water bath while preserving the first and last sections. I've been sectioning paraffin blocks for almost 4 years now. However, I've never needed to cut serial sections and the samples were always large. So, I never worried about losing the first section of each ribbon (and sometimes the last). I would like to know what techniques the histologists out there use to transfer the ribbon from the microtome to the water bath. I've used metal forceps and my fingers (gloved and not gloved) to grab the first section (but the section sticks to the forceps), and I'm using the wooden part of a cotton swab to lift off the last section, which is working fairly well. I'm also using a new microtome, Leica RM2235, and having some trouble with the first section coming off the knife just wrinkling up. And, the surface below the knife is such that even if I grab the edge of the section with a paintbrush, it won't budge. Is there anything I can do to correct these problems? Also, which is preferred, to cut the blocks chilled or to leave them RT. I used to cut blocks chilled all the time, but here it's preferred that I cut them RT unless I have a problem. Thanks, Jackie From mari.ann.mailhiot <@t> leica-microsystems.com Fri Jul 30 14:54:22 2004 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] transferring paraffin ribbon Message-ID: Hi Jackie Give me a call and we can discuss what is happening with your paraffin ribbon. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Jacqueline Miller" ern.edu> cc: Sent by: Subject: [Histonet] transferring paraffin ribbon histonet-bounces@lists.utsouth western.edu 07/30/2004 02:16 PM Hi everyone, I would like to know about the techniques that histotechs use to cut paraffin sections--especially how the ribbon is transferred from microtome to water bath while preserving the first and last sections. I've been sectioning paraffin blocks for almost 4 years now. However, I've never needed to cut serial sections and the samples were always large. So, I never worried about losing the first section of each ribbon (and sometimes the last). I would like to know what techniques the histologists out there use to transfer the ribbon from the microtome to the water bath. I've used metal forceps and my fingers (gloved and not gloved) to grab the first section (but the section sticks to the forceps), and I'm using the wooden part of a cotton swab to lift off the last section, which is working fairly well. I'm also using a new microtome, Leica RM2235, and having some trouble with the first section coming off the knife just wrinkling up. And, the surface below the knife is such that even if I grab the edge of the section with a paintbrush, it won't budge. Is there anything I can do to correct these problems? Also, which is preferred, to cut the blocks chilled or to leave them RT. I used to cut blocks chilled all the time, but here it's preferred that I cut them RT unless I have a problem. Thanks, Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From gentras <@t> vetmed.auburn.edu Fri Jul 30 16:21:06 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:23:49 2005 Subject: Fwd: Re: [Histonet] Rodent Processing Schedule? Message-ID: <6.0.1.1.0.20040730162057.02574c30@mailhost.vetmed.auburn.edu> >Date: Fri, 30 Jul 2004 16:20:34 -0500 >To: "Timothy R. Wheelock" >From: "Atoska S. Gentry" >Subject: Re: [Histonet] Rodent Processing Schedule? > > > >Hello, please will you share your replies with me. I was about to make a >similar inquiry, except mine is from hindsight. I processed a couple of >whole rat brains on my four hour serial section rodent tissue process >cycle and unfortunately I'm experiencing hollow spaces in areas of the >brain upon sectioning. I'm wondering if this is a result of either >inadequate fixation or processing. Thanks, Atoska > > >At 08:48 AM 7/30/04, you wrote: >>Hi Everyone: >> >>Does anyone have a protocol for processing whole, formalin fixed mouse >>brains? The mouse brains need to be processed whole. >>I am concerned that over-processing, using a schedule meant for human >>brain tissue, will dry the tissue out excessively and make the serial >>sectioning a nightmare. >>Also, would the same schedule apply to whole rat brains, or should the >>times be increased a bit? >>Thank you. >> >>Tim Wheelock >>Harvard Brain Tissue Resource Center >>McLean Hospital >>Belmont MA >> >> >> >>Any information, including protected health information (PHI), transmitted >>in this email is intended only for the person or entity to which it is >>addressed and may contain information that is privileged, confidential and or >>exempt from disclosure under applicable Federal or State law. Any review, >>retransmission, dissemination or other use of or taking of any action in >>reliance upon, protected health information (PHI) by persons or entities >>other >>than the intended recipient is prohibited. If you received this email in >>error, >>please contact the sender and delete the material from any computer. >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From gentras <@t> vetmed.auburn.edu Fri Jul 30 16:41:50 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:23:49 2005 Subject: Fwd: Re: [Histonet] Rodent Processing Schedule? Message-ID: <6.0.1.1.0.20040730164142.025f2eb0@mailhost.vetmed.auburn.edu> >To: Gayle Callis >From: "Atoska S. Gentry" >Subject: Re: [Histonet] Rodent Processing Schedule? > > >Hello Gayle, by now you may have seen my forward to this inquiry. However, >the brains I have were fixed in PFA ( I'm not sure what percentage, maybe >15%), for approximately 3 weeks prior to processing. Also, please what are >the immersion times on your alcohol & clearing agents? Thanks, Atoska > >At 10:16 AM 7/30/04, you wrote: >>No, whole rat brains are larger and would require a longer processing >>schedule. >> >>We have processed whole mouse brains using automated VIP (Sakura >>Finetek) with NO TEMPERATURE added to dehydration stations nor clearing >>as this will add to tissue drying. >> >>Make sure your mouse brains are totally fixed, perfusion is ideal, >>followed by immersion overnight. >> >>We process 70, 80, 95 X 3, 100 X 3, Xylene X 1, Clearite 3 X 1 (or you >>can use 2 changes of Clearite 3), and 4 changes of paraffin 30 min X 2 >>changes, 1 hour x 2 changes. All stations are at 1 hour with exception >>of paraffins, just to shorten total time in that for 3 hours. Use vacuum >>and pressure for all stations, and do not exceed 60C with paraffin >>infiltration, avoiding excess heat helps. What you want to avoid is >>removing the bound water on proteins (this leads to hard, dry >>tissues) only free water in tissues spaces. Some labs like to have >>first dehydration stations at lower concentrations of alcohol, 50, 70, >>80, 95 X 2, 100 X 2 or 3, clearing, etc. Xylene tends to harden tissue, >>but Clearite 3 (Richard Allan) and Propar (ANATECH) are more forgiving, >>single aliphatic hydrocarbon xylene substitutes. These are only >>guidelines as your conditions may be slightly different. >> >>Recommended is do some processing runs on normal brain just to see what >>is optimal for your fixation and processor conditions. We recently had >>to customize processing schedules after some test runs for both >>Periodiate/lysine/paraformaldehyde perfused hamster brain (coronal >>sections) and tongue, with a separate schedule for mouse coronal brain >>sections. >> >>At 07:48 AM 7/30/2004, you wrote: >>>Hi Everyone: >>> >>>Does anyone have a protocol for processing whole, formalin fixed mouse >>>brains? The mouse brains need to be processed whole. >>>I am concerned that over-processing, using a schedule meant for human >>>brain tissue, will dry the tissue out excessively and make the serial >>>sectioning a nightmare. >>>Also, would the same schedule apply to whole rat brains, or should the >>>times be increased a bit? >>>Thank you. >>> >>>Tim Wheelock >>>Harvard Brain Tissue Resource Center >>>McLean Hospital >>>Belmont MA >>> >>> >>> >>>Any information, including protected health information (PHI), transmitted >>>in this email is intended only for the person or entity to which it is >>>addressed and may contain information that is privileged, confidential >>>and or >>>exempt from disclosure under applicable Federal or State law. Any review, >>>retransmission, dissemination or other use of or taking of any action in >>>reliance upon, protected health information (PHI) by persons or entities >>>other >>>than the intended recipient is prohibited. If you received this email in >>>error, >>>please contact the sender and delete the material from any computer. >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>Gayle Callis >>MT,HT,HTL(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University - Bozeman >>PO Box 173610 >>Bozeman MT 59717-3610 >>406 994-6367 (lab with voice mail) >>406 994-4303 (FAX) >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From JColCLEFA <@t> aol.com Fri Jul 30 17:06:43 2004 From: JColCLEFA <@t> aol.com (JColCLEFA@aol.com) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] 10 hour days- if only! Message-ID: <1BD099E9.1022BB56.0229601F@aol.com> We routinely work 10 hour days 5 days a week! A scheduled 10 hour work day four days a week sounds like a sweet deal. Sign me up! Actually we tried it out once or twice and the administration of the hospital didn't like it because some of the techs on different shifts where 10 hours would not be practical felt it was unfair to them. Boo Hoo! From schaef <@t> cablelan.net Fri Jul 30 18:37:07 2004 From: schaef <@t> cablelan.net (Don & Cathy) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] messages Message-ID: <000b01c4768e$26527bf0$b1a68e8b@OWNERK0N3ZT9M2> Is the Histonet still operating? From RSRICHMOND <@t> aol.com Fri Jul 30 21:33:20 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] scalpel blades for grossing Message-ID: <105.4cb9fbaf.2e3c5ef0@aol.com> This old pathologist wants a blade with a sharp point for cutting sutures and testing the surfaces of abraded articular cartilage for eburnation. I think one of the regulatory agencies is trying to take these away from us - can't remember which one. Once when I for a pair of sharp scissors instead of the standard gross desk scissors, with sprung hinge and blades too dull to cut a gallbladder, I was told I could have them if I signed a promise not to run with them! On a related topic, I looked into the price of six or eight inch long disposable blades - I used these in one lab and liked them very much. Found out they cost about ten dollars each - totally out of the question, even if one can hold one's use of them down to one a day. (Can you imagine a surgeon putting up with such a restriction?) Bob Richmond Gastonia NC and Knoxville TN From slimwillie <@t> cox.net Sat Jul 31 19:56:49 2004 From: slimwillie <@t> cox.net (Jerry Wilson) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Securline markers References: Message-ID: <003a01c47762$6d874b20$f2340e44@no.cox.net> I second the StatMark Pens from StaLab. They are great. Jerry ----- Original Message ----- From: "Bartlett, Jeanine" To: "Emily" ; Sent: Monday, July 26, 2004 12:58 PM Subject: RE: [Histonet] Securline markers Always a problem. Try the Statmart pens from StatLab. Maybe Leslie will send you a free sample! 1-800-442-3573 X225 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sent: Monday, July 26, 2004 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Securline markers We have been having a major problem the Securline markers for about a year - they are pretty much dry when we get them. The company that manufactures them told me to add xylene to each marker. It works most of the time, but it takes up a lot of time to add the xylene. My feeling is that I shouldn't have to do that anyway - they should just make a marker that actually works. I have tried out many other pens/markers and have found none to be satisfactory. The ink either fades too much during processing or just totally washes off. Does anyone have any similar issues? Does anyone have any ideas or suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Wed Jul 28 14:11:41 2004 From: tahseen <@t> brain.net.pk (Muhammad Tahseen) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Trimming path hood (Gross Lab) Message-ID: <000201c4777f$39fc3b40$972bfea9@m7c0y4> Dear all, Question: If you had to buy a new trimming path hood (Gross Lab) and you had to chose between a BIO-Optica or a Shandon, which would you purchase, and please tell me why. Mainly I would love to hear from anyone that has had these in the last 10 years. I really need your input on this, detailed input, especially in regards to when you bought the machine that you do not like and how long before it started giving you trouble. Thanks in advance, Muhammad Tahseen SKMH&RC From tahseen <@t> brain.net.pk Sat Jul 31 23:34:16 2004 From: tahseen <@t> brain.net.pk (Muhammad Tahseen) Date: Fri Sep 16 15:23:49 2005 Subject: [Histonet] Trimming path hood (Gross Lab) Message-ID: <004101c47781$c579d900$972bfea9@m7c0y4> Dear all, Question: If you had to buy a new trimming path hood (Gross Lab) and you had to chose between a BIO-Optica or a Shandon, which would you purchase, and please tell me why. Mainly I would love to hear from anyone that has had these in the last 10 years. I really need your input on this, detailed input, especially in regards to when you bought the machine that you do not like and how long before it started giving you trouble. Thanks in advance, Muhammad Tahseen SKMH&RC