[Histonet] Muscle fiber differentiation

Marion Hiles Marion.Hiles <@t> north-bristol.swest.nhs.uk
Thu Jan 29 10:14:03 CST 2004


We use Myosin Slow & Myosin Fast from Novocastra which work best on frozen
sections. Some degree of staining on FFPE after microwaving in citrate pH6
buffer.

Bob Quilty
Dept of Neuropathology
Frenchay Hospital
Bristol  UK

-----Original Message-----
From: Susan Q Wells [mailto:susan.wells <@t> bms.com]
Sent: 28 January 2004 13:00
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Muscle fiber differentiation


Does anyone have any ideas on how to distinguish Type 1 and Type
2 muscle fibers with IHC? We have done  an ATPase  stain but
wanted to know if there are available antibodies?
Thanks in advance for your help,
Sue Wells HT(ASCP),QIHC


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