[Histonet] Re: DAB precipitate

[Johnson, Teri]

Anila Syed wrote: >I have been using DAB successfully for a while, but
today when I stained my sections, there were huge clumps of DAB
precipitates stuck all over the section. I didn't do anything different
and it was a brand new kit. I am sure that I mixed it well as I vortex
for about ten seconds prior to use.<

You didn't say what vendor you get yours from.  We had that problem with
Dako's DAB+, we opened a kit that started precipitating on us.  We
complained to them and they sent a replacement and haven't had that
problem with it since.

I'd suggest you contact the vendor and let them know what is happening.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org