From DMBCMP <@t> aol.com Thu Jan 1 14:44:44 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Cytotechnologist Position Available Message-ID: <139.29a4683b.2d25e0bc@aol.com> Cytotechnologist position available at Fresno Community Hospital, Fresno, California. If interested, please call 559.459.1845..... Ask for Charles A. Mendoza, Mngr., Dept. of Pathology. This listing submitted on HistoNet for Charles by Dannie Blake, HT (ASCP) Histology Lab Technical Lead, Community Hospital. From DMBCMP <@t> aol.com Thu Jan 1 15:00:10 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Steve Slap Please Read Message-ID: <154.2ae04d0f.2d25e45a@aol.com> Hello, Steve: No matter what, you are still the microwave specialist and I need your input, if you would be so gracious as to reply. Our Milestone Renaissance is being used daily for a run of placenta blocks. (I know bloody specimens are not highly recommended for microwave.) Do you have any idea why paraffin is being sucked into the vacuum tube? This is happening at various times....not necessarily when it has a full load. Our first run of biopsies is fine. The placentas are on the second run. The paraffin is up to temp @ 80 C. We were wondering if the problem is simply because they ARE bloody tissue and we just need to change the paraffin if we wish to use the microwave for this application. What do you think? I sure would appreciate your expert knowledge on this. NOTE: Anyone else who has any ideas (Tim Morken, during your CDC days?) .... you are most welcome to jump in. Steve, I hope you had a good holiday. Happy 2004!!!! Same to all you people around the world who read and learn from HistoNet. HAPPY NEW YEAR!!! Thanks, Dannie Blake, HT (ASCP) Sierra Pathology, Fresno Community Hospital Fresno, California From haldana <@t> unimoron.edu.ar Fri Jan 2 04:04:15 2004 From: haldana <@t> unimoron.edu.ar (hernan) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] unsuscribe References: <154.2ae04d0f.2d25e45a@aol.com> Message-ID: <001a01c3d117$cc39d120$0c14a8c0@pc> unsuscribe From Loralee_Gehan <@t> URMC.Rochester.edu Fri Jan 2 07:16:38 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Steve Slap Please Read Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804A4@exmc3.urmc.rochester.edu> I have this wonderful hacker machine as well and I had this problem several weeks ago. I called the company and it was suggested to me that the cassettes may have cooled a bit before they were put into paraffin, like at the vacuum step. This made sense to me because, after the vacuum step I didn't put the cassettes directly into paraffin, the phone rang and I went to answer it. So maybe the cassettes did cool for a minute or two before they were put into paraffin. I had paraffin along the lid and in the tubing. What a mess. I pulled everything apart and ran hot hot water over it to clean it the best I could. I haven't had a problem since then. Hope this helps a little. Regards, Loralee Gehan Orthopaedics Research Lab University of Rochester > ---------- > From: DMBCMP@aol.com > Sent: Thursday, January 1, 2004 4:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Steve Slap Please Read > > Hello, Steve: > No matter what, you are still the microwave specialist and I need your > input, > if you would be so gracious as to reply. > > Our Milestone Renaissance is being used daily for a run of placenta > blocks. > (I know bloody specimens are not highly recommended for microwave.) Do you > have > any idea why paraffin is being sucked into the vacuum tube? This is > happening at various times....not necessarily when it has a full load. Our > first run > of biopsies is fine. The placentas are on the second run. The paraffin > is up > to temp @ 80 C. We were wondering if the problem is simply because they > ARE > bloody tissue and we just need to change the paraffin if we wish to use > the > microwave for this application. What do you think? I sure would > appreciate your > expert knowledge on this. > NOTE: Anyone else who has any ideas (Tim Morken, during your CDC days?) > .... > you are most welcome to jump in. > Steve, I hope you had a good holiday. Happy 2004!!!! > Same to all you people around the world who read and learn from HistoNet. > HAPPY NEW YEAR!!! > > Thanks, > Dannie Blake, HT (ASCP) > Sierra Pathology, Fresno Community Hospital > Fresno, California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From pgoldberg <@t> hanespc.com Fri Jan 2 09:33:31 2004 From: pgoldberg <@t> hanespc.com (Paul Goldberg) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Unsuscribe Message-ID: Unsuscribe From micro <@t> formatex.org Thu Jan 1 10:27:20 2004 From: micro <@t> formatex.org (Microscopy Book Series) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Call for Chapters for Microscopy book: Kluwer/Formatex Message-ID: Dear Colleague, Formatex, a Spanish Research Center, in association with Kluwer Academic Publishers, is now preparing the Second number of the Formatex Microscopy book series, with the preliminary title of "Current Issues in Multidisciplinary Microscopy Research and Education". You can see the contents of the first number published in 2003 in http://www.formatex.org/micro2002/callforpaper.htm Website of the 2004 edition is http://www.formatex.org/micro2003/callforpaper2.htm This second number of the series is committed to giving an overview of the state of the art as well as upcoming trends, and to promoting discussion about scientific, technological and educational aspects of Microscopy in both the biological/biomedical and physical/chemical sciences. Although all types of papers are a priori accepted (research articles, reviews, case studies, etc.), priority will be given to those which clearly emphasize the scientific/technological/pedagogical results, as well as those making comparative discussions of two or more microscopy techniques or showing the complementarity of microscopy techniques with other techniques. For this second number, "educationally-oriented" and mini-review papers are specially welcome, although also "regular" research papers are accepted. If you are interested in participating in this edition submitting a (technical, scientific, educational, introductory...) chapter related to microscopy, please see the website for details. As you may see from the Call for Papers' website, the deadline for chapter submission is MARCH 15TH. Early submission of a short abstract of your chapter proposal is appreciated in order to allow potencial authors to know what other authors will write about for the book and avoid contents duplications. We hope that you find this new approach to microscopy issues interesting and we hope to hear from you/your team for this and/or future editions. If you any enquiry or suggestion about this volume, please contact us. Best wishes from Spain. Jos? Antonio Mesa Gonzalez Editorial Assistant Formatex Research Center C / Encarnacion, 3 1?E 06001 Badajoz SPAIN Phone/Fax: +34 924258615 Email: micro@formatex.org (If you do not want to receive the next reminder about this book series, please reply this message with "remove" in the subject). From RFORD <@t> HCMHCARES.ORG Fri Jan 2 12:04:10 2004 From: RFORD <@t> HCMHCARES.ORG (Ford, Rhonda) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Clearing through xylene & cover slipping with xylene substitute Message-ID: <684DF8CDE1FB6A428499DA65D753D12224BEDB@jupiter.hcmh.org> Do any of you clear your slides through xylene but coverslip using a xylene substitute? If so what xylene substitute do you use and what mounting medium? We have an employee who feels that her dermatitis is due to cover slipping from xylene. We are also considering gloves. From Myri37 <@t> aol.com Fri Jan 2 12:38:22 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Epon resin Message-ID: <16b.284817a3.2d27149e@aol.com> Dear histonetters I wish you a healthy and happy new year 2004 !! Does anyone know difference between Epon done with (epoxy 218, NMA, DDSA, DPM30) and Epon done with ( epoxy218, araldite 502, DDSA, DMP30) wich one do you think is better for embedding thin human tissue ? thank you Myriam Natural Implant From stevemachinuk <@t> yahoo.co.uk Fri Jan 2 14:21:36 2004 From: stevemachinuk <@t> yahoo.co.uk (=?iso-8859-1?q?Steve=20Machin=20UK?=) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Clearing through xylene & cover slipping with xylene substitute In-Reply-To: <684DF8CDE1FB6A428499DA65D753D12224BEDB@jupiter.hcmh.org> Message-ID: <20040102202136.49543.qmail@web25103.mail.ukl.yahoo.com> If the data supplied with your xylene says wear gloves then you must wear them but don't use latex gloves because they are not resistant and some people are alergic to latex. We used d-limonene(an product from organge skins) for about 10 years. The stained slides faded so we stopped using it. --- "Ford, Rhonda" wrote: > Do any of you clear your slides through xylene but coverslip using > a > xylene substitute? If so what xylene substitute do you use and > what > mounting medium? We have an employee who feels that her dermatitis > is > due to cover slipping from xylene. We are also considering gloves. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Yahoo! Messenger - Communicate instantly..."Ping" your friends today! Download Messenger Now http://uk.messenger.yahoo.com/download/index.html From rgarcia <@t> errc.ars.usda.gov Fri Jan 2 16:26:59 2004 From: rgarcia <@t> errc.ars.usda.gov (Rafael Garcia) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] most abundent proteins in whole body (or particular tissue types) Message-ID: I've exhausted myself looking for some information that seems like it would be pretty easy to find -- a list or ranking of the most abundant proteins in the whole body of a mammal (ideally the cow). One can think of some of the proteins that should be on the list - the various types of collagen, osteocalcin, keratin, elastin, actin, myosin, serum albumin, titin, hemoglobin. But I've been unable to find even a rough ranking. I've found some information on the most abundant proteins in particular tissues (skeletal muscle and bone), but even this is hard to come by. If have this type of information (whole body or particular tissue), please help me out! Rafael Garcia Chemical Engineer USDA-ARS Eastern Regional Research Center 600 East Mermaid Lane Wyndmoor PA 19038 voice: 215-836-3743 fax: 215-233-6795 rgarcia@errc.ars.usda.gov From cfavara <@t> niaid.nih.gov Fri Jan 2 17:12:29 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] RCA 120 & laminin Message-ID: All, Anyone doing staining using either the lectin RCA120 for activitated microglia or laminin antibody on mouse or hamsters? Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 From Nancy.Walker <@t> sanofi-synthelabo.com Sat Jan 3 08:03:07 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:23 2005 Subject: =?iso-8859-1?Q?R=E9f=2E_=3A_Re=3A_[Histonet]_Negative_response_from_the?= Journal of Neuropathology and Experimental Neurology Message-ID: Well said Dana, what an inspiration!! From dmikita <@t> wmcnet.org Sat Jan 3 11:00:00 2004 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] New Employee Histology Tech. Check off list Message-ID: Hello, Does anyone out there have a check off list for a new histology tech. We are going to be hiring a new histotech and haven't done this for about 10 years and we don't have a check off list. Our lab director would like us to have a check off list for them, when we do hire a tech. If you do have one you can email it to me at dmikita@wmcnet.org or fax it to my attention at 1(307)577-2371. Thanks, Daryl Mikita, HT(ASCP) From lesley <@t> vancouverbc.net Sat Jan 3 11:21:57 2004 From: lesley <@t> vancouverbc.net (Lesley Weston) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Epon resin In-Reply-To: <16b.284817a3.2d27149e@aol.com> Message-ID: Depends on which tissue you are embedding. The araldite mix is a little softer and more plastic than the NMA mix, so it's good for soft tissues. But bone needs a harder medium, as do many implant materials, even after decalcification, so the NMA mix works better. Lesley Weston. on 02/01/2004 10:38 AM, Myri37@aol.com at Myri37@aol.com wrote: > Dear histonetters > I wish you a healthy and happy new year 2004 !! > Does anyone know difference between Epon done with (epoxy 218, NMA, DDSA, > DPM30) and Epon done with ( epoxy218, araldite 502, DDSA, DMP30) > wich one do you think is better for embedding thin human tissue ? > thank you > Myriam > Natural Implant > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Sun Jan 4 18:05:11 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] most abundent proteins in whole body (or particulartissue types) Message-ID: <494304423C63E246A5CF87A3AEEB577011B5C2@bumail01.barrynet.barry.edu> I do not remember the source, but I have been told that the most abundant protein in the human (or other mammalian) body is collagen I. Most of the dry weight of a mammal is in the muscles. Since myosin is actually two proteins and there are two or three isoforms of it, skeletal muscle alpha actin is probably the second most abundant protein in the mammalian body. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rafael Garcia Sent: Friday, January 02, 2004 5:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] most abundent proteins in whole body (or particulartissue types) I've exhausted myself looking for some information that seems like it would be pretty easy to find -- a list or ranking of the most abundant proteins in the whole body of a mammal (ideally the cow). One can think of some of the proteins that should be on the list - the various types of collagen, osteocalcin, keratin, elastin, actin, myosin, serum albumin, titin, hemoglobin. But I've been unable to find even a rough ranking. I've found some information on the most abundant proteins in particular tissues (skeletal muscle and bone), but even this is hard to come by. If have this type of information (whole body or particular tissue), please help me out! Rafael Garcia Chemical Engineer USDA-ARS Eastern Regional Research Center 600 East Mermaid Lane Wyndmoor PA 19038 voice: 215-836-3743 fax: 215-233-6795 rgarcia@errc.ars.usda.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Ellin13 <@t> adelphia.net Sun Jan 4 18:52:17 2004 From: Ellin13 <@t> adelphia.net (Jesus Ellin) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Tamtron (HELP!!!) Message-ID: <003b01c3d326$2af7b280$6d2e4344@yumaaz.adelphia.net.losaca.adelphia.net> Hello there everyone thought that I would write to those that help me out with Tamtron and got me started but I am in a bit of a hole here,, our go live date has been pushed back and I am in need of some futher assistance,, We currently have the latest version of Tamron that is available ,, but we are having some difficulties with implementing the system,, our dilema is that we are going from a manual system that has been in place for over 50 yrs and going completely computerized and paperless,, also out of our 4 pathologist only 1 of them can turn on a computer,, I guess what I am getting at is, was anyone faced with the same dilema and if so what type of advice can you offer,,,??? I have had some really good starting points with paperwork and getting organized,, but what red flags should I be looking at before we lock down our system ,, and can anyone tell me what I should look out for or do to try and stream line the procces for the rest of the staff aka( Pathologist, Histo-Techs and Sect.) if you can please email me at : jellin@yumaregional.org all your help is appreciated Jesus Ellin From dmikita <@t> wmcnet.org Mon Jan 5 07:30:38 2004 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Clearing through xylene & cover slipping with xylene substitute Message-ID: Hello, We use the xylene substitute from Anatech Pro-Par (Cat.#511), and the use the mounting medium from American Master*Tech Clear*Mount (Cat.#MMCLEPT). Pro-Par doesn't have that citrus smell, and the Clear*Mount dried faster than other mounting mediums that we tried. Daryl Mikita, HT(ASCP) From siksik03 <@t> comcast.net Mon Jan 5 09:24:22 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Steve Slap Please Read In-Reply-To: <154.2ae04d0f.2d25e45a@aol.com> References: <154.2ae04d0f.2d25e45a@aol.com> Message-ID: Hi Dannie & HistoNetters Happy 2004 to All First, to address the side issue of finding histotechs on the Internet. Please don't forget that many of us (myself included) can be e-mailed directly from "The Directory of Histotechs on the Web" at http://www.histology.to. If you are not yet listed, but want tobe, there is a submission form available, or you can just send your listing to me at mailto:steven@hstology.to. Now, forgive me a brief plea. Peggy Wenk and I have maintained this web site, "The Histotech's Home Page", as a volunteer effort, for some years now. Over the years, we have been aided by the generous financial support of several commercial sponsors, but we are now down to one sponsor (thank you, Pacific Southwest Lab Supplies!!!), and this is not nearly enough to pay the bills associated with maintaining the site. As I am now self-employed, I cannot afford to support the site on my own. I hope that several companies can step forward in 2004 to help out and keep this valuable resource available. Now to Dannie's question. The purpose of the vacuum step in microwave histoprocessing is to remove all residual isopropanol from the tissue. The problem with the placentas, I think, is twofold. First, there may still be residual water in the tissue (try extending the isopropanol step). Second, there is a very large amount of alcohol being carried over within the tissue into the vacuum step into the paraffin. As the isopropanol flash evaporates, it is causing a "volcano" effect in the paraffin, and paraffin is splattering up into the vacuum. Both of these problems appear only with very large tissues, and would not be seen with biopsies. In addition to extending the isopropanol step, I would make sure that the paraffin is at the correct temperature at the start of the cycle, and is not overfilled as first steps to resolving the problem. Dannie- thanks for the compliment, and feel free to contact me directly. best regards, Steven Slap Microwave Consultant At 4:00 PM -0500 1/1/04, DMBCMP@aol.com wrote: >Hello, Steve: >No matter what, you are still the microwave specialist and I need your input, >if you would be so gracious as to reply. > >Our Milestone Renaissance is being used daily for a run of placenta blocks. >(I know bloody specimens are not highly recommended for microwave.) >Do you have >any idea why paraffin is being sucked into the vacuum tube? This is >happening at various times....not necessarily when it has a full >load. Our first run >of biopsies is fine. The placentas are on the second run. The paraffin is up >to temp @ 80 C. We were wondering if the problem is simply because they ARE >bloody tissue and we just need to change the paraffin if we wish to use the >microwave for this application. What do you think? I sure would >appreciate your >expert knowledge on this. >NOTE: Anyone else who has any ideas (Tim Morken, during your CDC days?) .... > you are most welcome to jump in. >Steve, I hope you had a good holiday. Happy 2004!!!! >Same to all you people around the world who read and learn from HistoNet. >HAPPY NEW YEAR!!! > >Thanks, >Dannie Blake, HT (ASCP) >Sierra Pathology, Fresno Community Hospital >Fresno, California > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From siksik03 <@t> comcast.net Mon Jan 5 09:36:58 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Epon resin In-Reply-To: <16b.284817a3.2d27149e@aol.com> References: <16b.284817a3.2d27149e@aol.com> Message-ID: Hi Myriam & HistoNetters This is actually a very complicated question, depending on quite a lot of factors. However, the one suggestion I would certainly make is to substitute BDMA for DMP-30 as the accelerator. It has a viscosity of 0.85 cP at 25?C compared to DMP-30 (20.50 cP). If you are going to use Araldite, I recommend you consult Audrey Glauert's books. I usually use Luft's NMA/DDSA formula, except with BDMA. best regards, Steven Slap At 1:38 PM -0500 1/2/04, Myri37@aol.com wrote: >Dear histonetters >I wish you a healthy and happy new year 2004 !! >Does anyone know difference between Epon done with (epoxy 218, NMA, DDSA, >DPM30) and Epon done with ( epoxy218, araldite 502, DDSA, DMP30) >wich one do you think is better for embedding thin human tissue ? >thank you >Myriam >Natural Implant >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DonnaWillis <@t> texashealth.org Mon Jan 5 09:48:18 2004 From: DonnaWillis <@t> texashealth.org (Willis, Donna) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Steve Slap Please Read Message-ID: <5C6CBCCEB04B894C8BD15B312487F7B2205B0D@ftwex01.txhealth.org> Dannie, We process placentas daily in our Milestone RHS1 unit without any problems. I can send you the protocol via fax if you will send me a number or by e-mail if your system will allow you to receive attachments. Donna Willis Histology Lab Manager Harris Methodist Fort Worth, Tx -----Original Message----- From: Steven E. Slap [mailto:siksik03@comcast.net] Sent: Monday, January 05, 2004 9:24 AM To: DMBCMP@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Steve Slap Please Read Hi Dannie & HistoNetters Happy 2004 to All First, to address the side issue of finding histotechs on the Internet. Please don't forget that many of us (myself included) can be e-mailed directly from "The Directory of Histotechs on the Web" at http://www.histology.to. If you are not yet listed, but want tobe, there is a submission form available, or you can just send your listing to me at mailto:steven@hstology.to. Now, forgive me a brief plea. Peggy Wenk and I have maintained this web site, "The Histotech's Home Page", as a volunteer effort, for some years now. Over the years, we have been aided by the generous financial support of several commercial sponsors, but we are now down to one sponsor (thank you, Pacific Southwest Lab Supplies!!!), and this is not nearly enough to pay the bills associated with maintaining the site. As I am now self-employed, I cannot afford to support the site on my own. I hope that several companies can step forward in 2004 to help out and keep this valuable resource available. Now to Dannie's question. The purpose of the vacuum step in microwave histoprocessing is to remove all residual isopropanol from the tissue. The problem with the placentas, I think, is twofold. First, there may still be residual water in the tissue (try extending the isopropanol step). Second, there is a very large amount of alcohol being carried over within the tissue into the vacuum step into the paraffin. As the isopropanol flash evaporates, it is causing a "volcano" effect in the paraffin, and paraffin is splattering up into the vacuum. Both of these problems appear only with very large tissues, and would not be seen with biopsies. In addition to extending the isopropanol step, I would make sure that the paraffin is at the correct temperature at the start of the cycle, and is not overfilled as first steps to resolving the problem. Dannie- thanks for the compliment, and feel free to contact me directly. best regards, Steven Slap Microwave Consultant At 4:00 PM -0500 1/1/04, DMBCMP@aol.com wrote: >Hello, Steve: >No matter what, you are still the microwave specialist and I need your input, >if you would be so gracious as to reply. > >Our Milestone Renaissance is being used daily for a run of placenta blocks. >(I know bloody specimens are not highly recommended for microwave.) >Do you have >any idea why paraffin is being sucked into the vacuum tube? This is >happening at various times....not necessarily when it has a full >load. Our first run >of biopsies is fine. The placentas are on the second run. The paraffin is up >to temp @ 80 C. We were wondering if the problem is simply because they ARE >bloody tissue and we just need to change the paraffin if we wish to use the >microwave for this application. What do you think? I sure would >appreciate your >expert knowledge on this. >NOTE: Anyone else who has any ideas (Tim Morken, during your CDC days?) .... > you are most welcome to jump in. >Steve, I hope you had a good holiday. Happy 2004!!!! >Same to all you people around the world who read and learn from HistoNet. >HAPPY NEW YEAR!!! > >Thanks, >Dannie Blake, HT (ASCP) >Sierra Pathology, Fresno Community Hospital >Fresno, California > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From asmith <@t> mail.barry.edu Mon Jan 5 10:13:13 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Most abundant protein Message-ID: <494304423C63E246A5CF87A3AEEB577011EDCB@bumail01.barrynet.barry.edu> Collagen I is the most abundant protein. It accounts for 27% of the dry weight of the human body. Ref.: L.C. Junquiera & J. Carneiro: BASIC HISTOLOGY, 10th ed., 2003, Lange, NY, p. 106. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From mward <@t> wfubmc.edu Mon Jan 5 10:24:29 2004 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] CD52 Message-ID: <61135F0455D33347B5AAE209B903A30403262338@EXCHVS2.medctr.ad.wfubmc.edu> Is anyone doing CD52 on ffpe and if so could you let me know what vendor you are using? One of our Pathologists is inquiring. Thanks in advance for any help you can give me. Martha Ward, MT (ASCP) QIHC Wake Forest University Baptist Medical Center From mcauliff <@t> umdnj.edu Mon Jan 5 10:45:53 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Epon resin In-Reply-To: <16b.284817a3.2d27149e@aol.com> References: <16b.284817a3.2d27149e@aol.com> Message-ID: <3FF994C1.3080806@umdnj.edu> Hi Myri: I don't know if one is better than the other and I suspect that in most uses these mixtures would be indistinguishable. I do concur with Steve's suggestion of using BDMA instead of DMP-30. The former has a longer shelf life. Geoff Myri37@aol.com wrote: >Dear histonetters >I wish you a healthy and happy new year 2004 !! >Does anyone know difference between Epon done with (epoxy 218, NMA, DDSA, >DPM30) and Epon done with ( epoxy218, araldite 502, DDSA, DMP30) >wich one do you think is better for embedding thin human tissue ? >thank you >Myriam >Natural Implant >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From kosmicdog <@t> hotmail.com Mon Jan 5 10:52:26 2004 From: kosmicdog <@t> hotmail.com (jason madore) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] slide staining dishes? Message-ID: quick question. does anyone know if you can use solvent resistant containers (ie tissue-tek green containers) to put xylenes into or do you have to us polyprolene containers? thanks. j. _________________________________________________________________ MSN 8 helps eliminate e-mail viruses. Get 2 months FREE*. http://join.msn.com/?page=features/virus&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From nklemme <@t> corp.sakuraus.com Mon Jan 5 12:20:05 2004 From: nklemme <@t> corp.sakuraus.com (Nancy Klemme) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] slide staining dishes? In-Reply-To: Message-ID: <001f01c3d3b8$8c376670$3b04100a@NancyK> Dear Jason, The "green" dishes that are used in the TissueTek manual stain setup are ACETAL COPOLYMER and were designed for holding xylene and xylene substitutes. The clear or "white" dishes are POLYETHYLENE and were designed for use with standard stains and reagents, but not xylene. Kind regards, Nancy Klemme Mgr, Technical Support Sakura Finetek USA, Inc. 800-725-8723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jason madore Sent: Monday, January 05, 2004 8:52 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slide staining dishes? quick question. does anyone know if you can use solvent resistant containers (ie tissue-tek green containers) to put xylenes into or do you have to us polyprolene containers? thanks. j. _________________________________________________________________ MSN 8 helps eliminate e-mail viruses. Get 2 months FREE*. http://join.msn.com/?page=features/virus&pgmarket=en-ca&RU=http%3a%2f%2f join.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- [This E-mail scanned for viruses by Declude Virus] --- [This E-mail scanned for viruses by Declude Virus] From jamie0139 <@t> bellsouth.net Mon Jan 5 12:40:11 2004 From: jamie0139 <@t> bellsouth.net (jamie0139@bellsouth.net) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] uric acid fixation and processing Message-ID: <20040105184011.YSPH1884.imf16aec.mail.bellsouth.net@mail.bellsouth.net> after fixing in 100% alcohol, what is processing and staining procedure for uric acid specimen. From jmitchell <@t> neurology.wisc.edu Mon Jan 5 13:15:40 2004 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean A.)) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Epon resin Message-ID: <061D5F3CF2DFDF4EADF5611CC1218C4512A659@nrl-lorenz.neurology.wisc.edu> I will have to disagree with using BDMA in place of DMP 30. I find that the final result using BDMA is chippy & brittle resin. NMA, DDSA, Epon with DMP 30 has given me consistant results for EM with a wide variety of tissue. DMP 30 can be purchased in smaller bottles of 25 ml if you are concerned with shelf life. Jean Mitchell University of Wisconsin Hospital & Clinics Department of Neurology, Neuromuscular Laboratory Madison, WI -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Monday, January 05, 2004 10:46 AM To: Myri37@aol.com Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] Epon resin Hi Myri: I don't know if one is better than the other and I suspect that in most uses these mixtures would be indistinguishable. I do concur with Steve's suggestion of using BDMA instead of DMP-30. The former has a longer shelf life. Geoff Myri37@aol.com wrote: >Dear histonetters >I wish you a healthy and happy new year 2004 !! >Does anyone know difference between Epon done with (epoxy 218, NMA, >DDSA, >DPM30) and Epon done with ( epoxy218, araldite 502, DDSA, DMP30) >wich one do you think is better for embedding thin human tissue ? >thank you >Myriam >Natural Implant >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nklemme <@t> corp.sakuraus.com Mon Jan 5 13:26:41 2004 From: nklemme <@t> corp.sakuraus.com (Nancy Klemme) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] slide staining dishes? In-Reply-To: Message-ID: <002b01c3d3c1$d9629010$3b04100a@NancyK> -CORRECTION- Dear Jason and other interested readers, I recognized an error in the information I submitted. The "green" dishes that are used in the TissueTek manual stain setup are ACETAL COPOLYMER and were designed for holding xylene and xylene substitutes. The clear or "white" dishes are POLYPROPYLENE (not polyethylene) and were designed for use with standard stains and reagents, but not xylene. Kind regards plus my apology, Nancy Klemme Mgr, Technical Support Sakura Finetek USA, Inc. 800-725-8723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jason madore Sent: Monday, January 05, 2004 8:52 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slide staining dishes? quick question. does anyone know if you can use solvent resistant containers (ie tissue-tek green containers) to put xylenes into or do you have to us polyprolene containers? thanks. j. _________________________________________________________________ MSN 8 helps eliminate e-mail viruses. Get 2 months FREE*. http://join.msn.com/?page=features/virus&pgmarket=en-ca&RU=http%3a%2f%2f join.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- [This E-mail scanned for viruses by Declude Virus] --- [This E-mail scanned for viruses by Declude Virus] From RITA.ANGEL <@t> UC.EDU Mon Jan 5 13:46:42 2004 From: RITA.ANGEL <@t> UC.EDU (Rita Angel) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] freezing mouse lungs Message-ID: <5.1.0.14.2.20040105144209.00b172c0@ucmail3.uc.edu> Hi all, I was wondering if anyone could offer help in obtaining the best sections possible with frozen mouse lungs. How can I prepare the lungs and freeze them to get the best results? I know this is difficult tissue to get good sections on anyway, with all the air pockets. Is it possible to infuse them with freezing embedding media? The lung sections I'm getting have a lot of holes, and at best, I'm only getting tissue from half of the lung samples while cutting. They were brought to me already frozen and embedded so I'm not sure how they were prepared. Thanks for your help, Rita Angel, HT University of Cincinnati From ldunikoski <@t> rbmc.org Mon Jan 5 14:36:03 2004 From: ldunikoski <@t> rbmc.org (Dunikoski, Leonard PhD) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Jon Opportunity: Histology Supervisor Message-ID: <7A2DF880A7A1D511A63300508BF7D5550271AC79@ES1> The Perth Amboy Division of Raritan Bay Medical Center is looking for a special person to be the Surgical Pathology Supervisor for our busy, computerized (CoPath) lab. The ideal candidate is a dynamic individual who: - has excellent inter-personal skills, with an ability to communicate effectively with technologists, patients, pathologists, other physicians and hospital staff. - has experience in immunohistochemistry - has demonstrated increasing amounts of responsibility during her/his career. - is committed to providing patient-focused care, as well as an excellent working environment. -is able to lead by example, be a coach and a cheerleader, and have a positive outlook on life and work. - is excited to lead a cytologist plus a staff of histotechnologists through inspections (CAP, JCAHO and state), customer relations goals (Press-Ganey), staff recruitment/retention goals, and all the other challenges we face in modern healthcare. If you feel you might be the ideal candidate, please send your curriculum vitae to: ldunikoski@rbmc.org Leonard K. Dunikoski, Ph.D. Director of Operations Raritan Bay Medical Center Old Bridge, NJ 08857 (732) 324-5163 NOTICE: The information included in this email contains confidential information belonging to the sender. This information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents hereof is strictly prohibited. If you have received this email in error, please notify the sender immediately. From gentras <@t> vetmed.auburn.edu Mon Jan 5 14:56:13 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] automatic tissue processors Message-ID: <5.2.0.9.0.20040105144533.00a010a0@mailhost.vetmed.auburn.edu> Hello, please I need input from the users of any/all of the tissue processors listed: 1. Leica TP 1020 Automatic Tissue Processor with Vacuum Infiltration & Fume Control; 2. Microm STP 120 Spin Tissue Processor; and/or 3. ThermoShandon Citadel 2000 Tissue Processor. If any of you will please provide me with contact information I will gladly call you. Your prompt replies will be much appreciated. Atoska Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From DMBCMP <@t> aol.com Mon Jan 5 19:47:03 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Microwave:To Steve Slap,Donna and Loralee Message-ID: <19e.1ed5bf97.2d2b6d97@aol.com> I want to thank you all for your informative responses. I am happy to know there is such a wealth of knowledge out there. Donna, I really would appreciate you FAXing your protocol to me at: 559.459.1021......my lab number. Please accept my apology for "renaming" the Milestone. Ha! Had alot on my little pea-brain at the time!! Best Wishes, Dannie Fresno Community Hospital From j.p.g.ooms <@t> zonnet.nl Tue Jan 6 02:41:01 2004 From: j.p.g.ooms <@t> zonnet.nl (Hans Ooms) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] RNA DNA (im)possibilities Message-ID: <000a01c3d430$d7a4db10$1d68a63e@st2c8pdli7fy3i> Dear Histonetters, First of all I Wish you all a prosperous and very healthy New Year!!!!! Secondly, the following question keeps me busy: what RELIABLE technique can be used to show RNA and DNA in paraffinsections ? Thanks in advance ! Hans Ooms HT (The Netherlands) From lpwenk <@t> covad.net Tue Jan 6 03:46:15 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] uric acid fixation and processing References: <20040105184011.YSPH1884.imf16aec.mail.bellsouth.net@mail.bellsouth.net> Message-ID: <002801c3d439$edaf0300$8f28a544@hppav> Go directly to xylene (or xylene substitute) and then into paraffin. Several changes of each, similar time sequence equivalent to routine processing of any other tissue the same size. Embed, section at 5 um, stain with a methenamine silver procedure. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Monday, January 05, 2004 1:40 PM Subject: [Histonet] uric acid fixation and processing > after fixing in 100% alcohol, what is processing and staining procedure for uric acid specimen. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ekaplan <@t> squ.edu.om Tue Jan 6 04:04:06 2004 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] plant histology Message-ID: Good afternoon, I wonder if there are any plant histologists that can give me a protocol for processing mango saplings? The area of interest in the woody stem. I would appreciate any help. Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman From dwalker <@t> selway.umt.edu Tue Jan 6 10:39:26 2004 From: dwalker <@t> selway.umt.edu (David Walker) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] RNA DNA (im)possibilities In-Reply-To: <000a01c3d430$d7a4db10$1d68a63e@st2c8pdli7fy3i> Message-ID: I have used both DAPI and Hoechst stains to stain DNA in FFPE tissues. Hoechst is fairly specific to DNA (it binds in the minor groove of double stranded DNA), although it also can bind double stranded sections of RNA (in ribosomes and transfer RNA). I am under the (mis?)conception that DAPI works in much the same way. Both of these are fluorescent dyes and they require UV light for excitation, and they both emit in the blue region of the spectrum. Propidium iodide is also a fluorescent dye that works to stain DNA and RNA. This dye excites at 536nm (green) and emits at 617nm (red). The Molecular Probes web site has information on these and other nucleic acid stains. Here is their web address: http://www.probes.com/handbook/sections/0801.html David Walker On 1/6/04 1:41, "Hans Ooms" wrote: > Dear Histonetters, > > First of all I Wish you all a prosperous and very healthy New Year!!!!! > > Secondly, the following question keeps me busy: > > what RELIABLE technique can be used to show RNA and DNA in paraffinsections ? > > Thanks in advance ! > > Hans Ooms > HT (The Netherlands) > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MTitford <@t> aol.com Tue Jan 6 10:48:23 2004 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] More on uric acid Message-ID: <0A946B33.43C353D8.00762DB1@aol.com> To clarify Peggy Wenk's comments, when you do the methenamine silver procedure, it is like a GMS but WITHOUT the oxidation. Go straight from deionized into the heated silver solution, and monitor it microscopically. A positive control is good to have! Our pathologists talk about positive and negative birefrigence of uric acid when looking at the H&E section with polarizing microscopy, but I can never get it straight in my mind! Mike Titford USA Pathology Mobile AL USA From Luis.Chiriboga <@t> med.nyu.edu Tue Jan 6 12:10:58 2004 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] RNA DNA (im)possibilities In-Reply-To: <000a01c3d430$d7a4db10$1d68a63e@st2c8pdli7fy3i> Message-ID: Methyl-green pyronin (MGP).... DNA=blue RNA=red. Very nice, good detail & works well in paraffin. Should be in any histology text. If not, pretty sure check AFIP staining manual. Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hans Ooms Sent: Tuesday, January 06, 2004 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RNA DNA (im)possibilities Dear Histonetters, First of all I Wish you all a prosperous and very healthy New Year!!!!! Secondly, the following question keeps me busy: what RELIABLE technique can be used to show RNA and DNA in paraffinsections ? Thanks in advance ! Hans Ooms HT (The Netherlands) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fmonson <@t> wcupa.edu Tue Jan 6 13:23:29 2004 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] RNA DNA (im)possibilities Message-ID: Afternoon Hans, I have a long-time favorite which works well for me on sections of Clarke's fixed material. There is no real credit for this except that I know from experience that it works nicely, AND is fast. ------------Thionin metachromasia of Nucleic Acids (Note: thionin preparations usually contain many related dyes such as Azure A, B and C. The point: Thionin, like methylene blue is not a pure chemical. In addition, metachromasia is a strange phenomenon that does not survive dilute alcohol rinses, thus the absence of 95% or lower ethanol dilutions. Clarke's is: 3(100% ethanol) + 1 (Glacial acetic acid) Dye is: 0.1% Thionin (C.I. 52000) in 100 ml glass distilled water Method 1. bring sections to water 2. immerse section in dye for 4 min 3. rinse in two changes of water (as above) 4. immerse in 100% (200 proof, U.S.P., undenatured!!) ethanol until no more color is removed (2-3 Coplin jars) 5. clear and mount as appropriate Result: DNA blue, RNA purple. ------------------------------------------------------------------------ ------------ A good histochemical procedure that I have used with RNase (DNase-free) and DNase (RNase free) is as follows: Flax and Himes, 1952(Ref if you want it?). Prep. 1. McIlvaine's Buffer at pH 4.0 (Pearse, 1968) a. 0.1M citric acid 122.9ml b. 0.2M disodium monobasic phosphate 77.1ml 2. Azure B (C.I. 52010): 0.25g / ml McIlvaine's Buffer, adjust to pH 4.0 Method 1. bring sections to water 2. treat control sections with appropriate procedures 3. rinse in water (always glass distilled 2x) 4. immerse in dye solution for 2 hr at 37oC 5. rinse in two changes of water 6. immerse in 100% tertiary butyl alcohol (TBA) for 16 hours 7. rinse in 2 changes of TBA for 1 hr each 8. mount as appropriate (my Damar mounts are good after 25 years*) * I know! Nobody cares after 2! Result: DNA crystal azure; RNA shades of purple. Methyl Green, Pyronin Y (Kurnick, 1952, J. Stain Technol, 27:233)) Prep 1. 0.2% aqueous Methyl Green (C.I. 42585) extracted with chloroform until no more violet color is observed in the chloroform partition. 2. saturated solution of Pyronin Y (C.I. 45005) in acetone Method 1. bring sections to water 2. immerse in MeGreen solution a. tissues fixed in Clarke's - 15 min b. Zenker acetic and Bouin's - 1 hr 3. rinse in two changes of n-butanol 4. immerse in pyronin Y for 10-15 sec 5. rinse rapidly in acetone 6. clear in cedar oil 7. clear again and mount as usual (again, I have always used Damar/Xylene) Results: DNA, green; RNA, pink Hope this helps, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Hans Ooms [mailto:j.p.g.ooms@zonnet.nl] Sent: Tuesday, January 06, 2004 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RNA DNA (im)possibilities Dear Histonetters, First of all I Wish you all a prosperous and very healthy New Year!!!!! Secondly, the following question keeps me busy: what RELIABLE technique can be used to show RNA and DNA in paraffinsections ? Thanks in advance ! Hans Ooms HT (The Netherlands) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From info <@t> instrumedics.com Tue Jan 6 14:26:45 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] lung frozens References: Message-ID: <008201c3d493$6879ce40$6501a8c0@INSTRUMEDICS22> Rita, Please visit our web site www.instrumedics.com to see how you can snap-freeze lung tissue with the Gentle Jane device and cut paraffin-quality frozen sections with the CryoJane Tape-Transfer system . Click on the "gallery" to see CryoJane prepared frozen sections among them lung frozens. If you have any questions please contact us. We welcome your questions. Bernice ----- Original Message ----- From: "Rita Angel" To: Sent: Monday, January 05, 2004 2:46 PM Subject: [Histonet] freezing mouse lungs > Hi all, > > I was wondering if anyone could offer help in obtaining the best sections > possible with frozen mouse lungs. How can I prepare the lungs and freeze > them to get the best results? I know this is difficult tissue to get good > sections on anyway, with all the air pockets. Is it possible to infuse them > with freezing embedding media? > > The lung sections I'm getting have a lot of holes, and at best, I'm only > getting tissue from half of the lung samples while cutting. They were > brought to me already frozen and embedded so I'm not sure how they were > prepared. > > Thanks for your help, > > Rita Angel, HT > University of Cincinnati > ----- Original Message ----- From: To: Sent: Tuesday, January 06, 2004 1:00 PM Subject: Histonet Digest, Vol 2, Issue 5 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > ---------------------------------------------------------------------------- ---- > Today's Topics: > > 1. RE: slide staining dishes? (Nancy Klemme) > 2. uric acid fixation and processing (jamie0139@bellsouth.net) > 3. RE: Epon resin (Mitchell (Jean A.)) > 4. RE: slide staining dishes? (Nancy Klemme) > 5. freezing mouse lungs (Rita Angel) > 6. Jon Opportunity: Histology Supervisor (Dunikoski, Leonard PhD) > 7. automatic tissue processors (Atoska S. Gentry) > 8. Microwave:To Steve Slap,Donna and Loralee (DMBCMP@aol.com) > 9. RNA DNA (im)possibilities (Hans Ooms) > 10. Re: uric acid fixation and processing (Lee & Peggy Wenk) > 11. plant histology (Evelyn Kaplan) > 12. Re: RNA DNA (im)possibilities (David Walker) > 13. More on uric acid (MTitford@aol.com) > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Tue Jan 6 14:42:56 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Bodian Stain Message-ID: <5.2.0.9.0.20040106142935.00a041e0@mailhost.vetmed.auburn.edu> Hello, a former colleague of mine who works in the only local human pathology lab is seeking input on the availability of a protocol for Bodian Stain other than the one in the AFIP manual. The reason they are seeking this is their lab is only equipped with one incubator and they routinely use it for drying slides at temperatures much higher than the required 48hour, 37C incubation of the protargol. They are equipped with a microwave for staining but are not sure how to use it for drying of slides without trial and error. Any suggestions provided will be much appreciated. Thanks, Atoska p.s. our lab is equipped but we only process animal tissues here. We've provided them with the chemicals they were lacking to complete this stain. Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From WWmn916 <@t> aol.com Tue Jan 6 20:23:05 2004 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Exam question Message-ID: <55.4e60765d.2d2cc789@aol.com> Greetings everyone, A few people in our lab are signing up to take the histotech exam (especially before 2005 cutoff with highschool diploma). What are the requirements if the practical is completed but written retesting is done after Dec 2005? Do both parts of the exam have to be successfully completed before December 2005? Thanks, Deb King, HT Sacramento, CA From bhewlett <@t> cogeco.ca Tue Jan 6 22:48:52 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] More on uric acid References: <0A946B33.43C353D8.00762DB1@aol.com> Message-ID: <002f01c3d4d9$8e275540$6400a8c0@bryanmainbox> Mike, The sign of birefringence, i.e. determination of the vibration direction or Positive and negative birefringence, is used to distinguish between pseudogout (pyrophosphate crystals) and gout (uric acid or urate crystals). Both types of crystal are birefringent. A first order red compensator or Lambda plate is introduced diagonally (45 degrees) between the crossed polarizer and analyzer. The compensator has the vibration direction of the slow ray marked on its handle. If the long axis of the crystal in the section is aligned parallel to the compensator's vibration direction, by rotating the microscope stage, an interference colour will be seen due to addition or subtraction of small phase differences. A blue colour indicates addition or positive birefringence = pyrophosphate (pseudogout). A yellow colour indicates subtraction or negative birefringence = uric acid or urates (gout). N.B. Both types of crystal may be blackened by hexamine silver! It is best to avoid H&E stained sections for this procedure since the aqueous steps and the blueing alkali may remove urates. Dewax the sections thoroughly, 10 min X 3 xylenes since wax is birefringent, mount unstained sections in resin. If you must stain for location purposes use an alcoholic nuclear stain (weak 0.01% Methylene blue in 80% alcohol etc.). Regards, Bryan ----- Original Message ----- From: To: Sent: Tuesday, January 06, 2004 11:48 AM Subject: [Histonet] More on uric acid > To clarify Peggy Wenk's comments, when you do the methenamine silver procedure, it is like a GMS but WITHOUT the oxidation. > Go straight from deionized into the heated silver solution, and monitor it microscopically. A positive control is good to have! > > Our pathologists talk about positive and negative birefrigence of uric acid when looking at the H&E section with polarizing microscopy, but I can never get it straight in my mind! > > Mike Titford > USA Pathology > Mobile AL USA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Wed Jan 7 05:11:49 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Exam question References: <55.4e60765d.2d2cc789@aol.com> Message-ID: <003601c3d50f$0c29d640$8732fea9@hppav> Both the written and the practical have to be taken before Jan. 1, 2005. That means people have to apply BEFORE July 1, 2004, in order to take (at the latest) the practical due in Sept. 2004 and the written in Oct-Dec. 2004. A person can take an exam a total of 5 times. But there is a 5 year "clause" related to the high school on the job (HS OJT) route. If someone takes both parts before the Dec. 31, 2004 deadline, they have a total of 5 times to take it over a 5 year period, and still be under the "pre-2005 rules". So if someone takes both parts in 2004, doesn't pass one or either part, they would still have 4 more times to take it in 2005, 2006, 2007, and part of 2008 (depends when in 2004 they took the exam). And still be under the pre-2005 rules, of letting them take it with the HS OJT. If they had passed one part (say, the practical), then they just have to retake the other part (the written in this case) until they pass, or their total of 5 times is used up, or 5 years is used up. If, however, they only retake the one part, for example, 3 times during that 5 year period. That 3 retakes, plus the first time they took it, which means they have taken it 4 times. They still have 1 more try (to make the 5). However, if it is after the 5 year period from when they first took it (example, it's now 2009), they are now signing up to take the exam under the NEW HT rules. This means they must now have the associate degree with 20 credits of biology and chemistry and 1 year OJT, or they must be a graduate of a NAACLS-accredited HT program. After 5 tries, they cannot take the HT exam, ever. No matter what route they go through. They could get the baccalaureate degree and try the HTL exam, however. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Tuesday, January 06, 2004 9:23 PM Subject: [Histonet] Exam question > Greetings everyone, > > A few people in our lab are signing up to take the histotech exam (especially > before 2005 cutoff with highschool diploma). What are the requirements if > the practical is completed but written retesting is done after Dec 2005? Do > both parts of the exam have to be successfully completed before December 2005? > > Thanks, > Deb King, HT > Sacramento, CA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Wed Jan 7 05:32:28 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Tamtron (HELP!!!) References: <003b01c3d326$2af7b280$6d2e4344@yumaaz.adelphia.net.losaca.adelphia.net> Message-ID: <003f01c3d511$ee935860$8732fea9@hppav> We switched from a tab over to the next field computer program written specifically for our lab by the hospital IS to Tamtron/Windows/mouse. So at least our people knew computers, but not Windows. 1. MOUSE: The hardest adjustment for many of our people was learning to use the mouse, believe it or not. This was major frustration for many people. It takes a lot of eye-hand coordination. - How far do I move the mouse to the right to get the cursor to move to the right. - What do I do when I get to the right edge of the mouse pad, but the cursor is only in the center of the screen? - Moving the hand but watching the cursor, not the hand. - Right click or left click - One click or two clicks - Rhythm of the clicks - Clicking without moving the mouse, which causes the cursor to move, making you open up something you don't want. It seems basic, but if you don't know how to use a mouse, you can't use Windows at all. One suggestion that worked for some people - Leave the games on the computer for a while, and let people play Solitaire before you go live with Tamtron. They learn how to open and close files, use the mouse, click, drag, eye-hand coordination of the mouse/cursor, etc. (Learning can be fun scenario.) 2. VACATIONS: Go live at a time when most people are at work, not over holiday weeks (Christmas, Easter, 4th of July week, etc.), and not over heavy vacation months. The learning curve will slow everyone down. Work will fall behind. You will need everyone there, just to get the work done. And you don't want to have to retrain lots of people the next week, because they were off for the Memorial week. 3. SUPERVISORS: Make certain all supervisors know this Tamtron inside and out, and definitely know Windows/mouse. Have them take classes on Windows, if needed. During go live - no supervisors are allowed to take a vacation. They will be needed to put out fires and calm people down and help people remember what they saw during training. Supervisors will NOT get any usual work done during that first week or two. So they should not plan on writing policies, attending meetings, or doing bench work. This is hard to arrange, as the "go live" day will change, several times, before being implemented. Trust me on this. (Not just Tramtron, but any major change to computers, telephone systems, etc.) Every change is delayed by weeks to months.) 4. LUNCH: The week you go live - have lunch catered into the department every day. No one will have time to go to the cafeteria, and everyone will need a break/morale booster/something to look forward to. Make it pizza one day, subs another, broasted chicken and potatoes another, Chinese another, spaghetti, spare ribs, whatever is around your area. Make sure you have salads every day for dieters and vegetarians. And desserts - lots of chocolate chip cookies. Definitely a help with stress. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Jesus Ellin" To: Sent: Sunday, January 04, 2004 7:52 PM Subject: [Histonet] Tamtron (HELP!!!) Hello there everyone thought that I would write to those that help me out with Tamtron and got me started but I am in a bit of a hole here,, our go live date has been pushed back and I am in need of some futher assistance,, We currently have the latest version of Tamron that is available ,, but we are having some difficulties with implementing the system,, our dilema is that we are going from a manual system that has been in place for over 50 yrs and going completely computerized and paperless,, also out of our 4 pathologist only 1 of them can turn on a computer,, I guess what I am getting at is, was anyone faced with the same dilema and if so what type of advice can you offer,,,??? I have had some really good starting points with paperwork and getting organized,, but what red flags should I be looking at before we lock down our system ,, and can anyone tell me what I should look out for or do to try and stream line the procces for the rest of the staff aka( Pathologist, Histo-Techs and Sect.) if you can please email me at : jellin@yumaregional.org all your help is appreciated Jesus Ellin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmitchell <@t> neurology.wisc.edu Wed Jan 7 11:09:44 2004 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean A.)) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Hey - Glen Dawson Message-ID: <061D5F3CF2DFDF4EADF5611CC1218C4512A65E@nrl-lorenz.neurology.wisc.edu> Sorry to involve the histonet - but Glen could you please contact me? Thanks Jean Mitchell University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI jmitchell@neurology.wisc.edu From GDawson <@t> Milw.Dynacare.com Wed Jan 7 12:08:10 2004 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Creating an HT from a BS Message-ID: All, I know we've talked about this topic many times but I have a specific case I would appreciate some help with. There is a lab helper who has been performing various types of labwork for years and has been working in histology now for a couple of months. She has a BS in biology and I would like to help her become a registered histotech. My hope is that, with so much previous lab experience as well as the bachelor's degree under her belt, she can sneak in under the bench-trained tech category before the 2005 deadline. Taking an accredited HT program is not a possibility for her as she would need to relocate to a place that offers a program which she could not currently afford anyway. Could anyone give me an idea about the feasibility of this idea? Thank-you in Advance, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI From RBARNHART <@t> summithealth.org Wed Jan 7 13:36:50 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Iron stain Message-ID: What stain does anyone use for bone marrow aspirate, blood smears and tissue. Also how long does it take to do the entire stain. We purchased a kit and when we try to stain a fixed blood smear everything is washed away. Thanks in advance. Becky Barnhart rbarnhart@summithealth.org From CMCCOLLOUGH <@t> dnr.state.md.us Wed Jan 7 13:42:12 2004 From: CMCCOLLOUGH <@t> dnr.state.md.us (McCollough, Carol) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] autofluorescence in thraustochytrids (protists) Message-ID: <4D36F3AA1525DF408493B4E1FDE9EDE50B4188@centrevilleex.langroup.dnr.md> Greetings: I have a question on behalf of a colleague. She is observing autofluorescence in thraustochytrids fixed in Davidson's AFA. The paper she is using as a reference for the acriflavine staining technique used 3.7% formaldehyde in seawater with Lugol's iodine (no concentration specified) as the fixative, and autofluorescence did not occur. Would anyone comment as to whether a difference in fixation would induce autofluorescence? Does Lugol's iodine quench autofluorescence? Thanks for your insights. Regards - Carol ****************** Carol B. McCollough, HT/HTL (ASCP) Diagnostics & Histology Laboratory Manager Maryland Department of Natural Resources Cooperative Oxford Laboratory 904 S. Morris Street Oxford, Maryland 21654 cmccollough@dnr.state.md.us From HornHV <@t> archildrens.org Wed Jan 7 14:19:36 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Creating an HT from a BS Message-ID: Wouldn't she be eligible with her BS degree to take it anyway?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com] Sent: Wednesday, January 07, 2004 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creating an HT from a BS All, I know we've talked about this topic many times but I have a specific case I would appreciate some help with. There is a lab helper who has been performing various types of labwork for years and has been working in histology now for a couple of months. She has a BS in biology and I would like to help her become a registered histotech. My hope is that, with so much previous lab experience as well as the bachelor's degree under her belt, she can sneak in under the bench-trained tech category before the 2005 deadline. Taking an accredited HT program is not a possibility for her as she would need to relocate to a place that offers a program which she could not currently afford anyway. Could anyone give me an idea about the feasibility of this idea? Thank-you in Advance, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital. From MTitford <@t> aol.com Wed Jan 7 14:25:55 2004 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Protargol Message-ID: <0E92D399.3AE96B46.00762DB1@aol.com> Atoska just up the road from me in Auburn, Alabama, asks about protargol. We have not purchased any recently, but have always purchased Roques strong silver protein(Protargol) from Cell Point Scientific (800) 999 9734. It gives consistant results, but you have to incubate a long time at 37 degrees C Mike Titford USA Pathology Mobile AL From mrsgbd2001 <@t> yahoo.com Wed Jan 7 16:16:57 2004 From: mrsgbd2001 <@t> yahoo.com (Gareth Davis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Creating an HT from a BS In-Reply-To: Message-ID: <20040107221657.62929.qmail@web13309.mail.yahoo.com> I have a BS and 10 years experience, so I wrote the ASCP and found out that I am eligible to get my certification - without taking any HT courses. You do have to have a specific amount of Biology courses and I think at least 1 year experience in HT within the last 5 years to be eligible. Check out the ASCP website at www.ascp.org to get the information you need. Gareth "Dawson, Glen" wrote: All, I know we've talked about this topic many times but I have a specific case I would appreciate some help with. There is a lab helper who has been performing various types of labwork for years and has been working in histology now for a couple of months. She has a BS in biology and I would like to help her become a registered histotech. My hope is that, with so much previous lab experience as well as the bachelor's degree under her belt, she can sneak in under the bench-trained tech category before the 2005 deadline. Taking an accredited HT program is not a possibility for her as she would need to relocate to a place that offers a program which she could not currently afford anyway. Could anyone give me an idea about the feasibility of this idea? Thank-you in Advance, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From gcallis <@t> montana.edu Wed Jan 7 17:33:46 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] BS to Histotech can be done without moving! Message-ID: <3.0.6.32.20040107163346.00bc4c08@gemini.msu.montana.edu> You Wrote: I know we've talked about this topic many times but I have a specific case I would appreciate some help with. There is a lab helper who has been performing various types of labwork for years and has been working in histology now for a couple of months. She has a BS in biology and I would like to help her become a registered histotech. My hope is that, with so much previous lab experience as well as the bachelor's degree under her belt, she can sneak in under the bench-trained tech category before the 2005 deadline. Taking an accredited HT program is not a possibility for her as she would need to relocate to a place that offers a program which she could not currently afford anyway. Could anyone give me an idea about the feasibility of this idea? Thank-you in Advance, **************************************************************** She can enroll in a histotech program while working in your lab, it is excellent and well worth the effort, time and money. Contact Glenda Hoye at Indiana University (ghoye@iupui.edu) none of her students are on site at the university but work under the auspices of a trained HT at their labs. What a wonderful way to become a registered histotech if one cannot relocate and there may be other programs available and comparable to Glenda's. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Jan 7 17:43:39 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Freezing mouse lungs Message-ID: <3.0.6.32.20040107164339.00bc4c08@gemini.msu.montana.edu> I recently put out an answer to your question, if not, here is the technic I sent off today to a gentleman who had problems. As for mouse lung, you euthanize mouse with anesthetic as cervical dislocation disrupts head and neck tissues needed for injection of OCT. Open the abdomen, severe veins and arteries behind intestines to bleed out animal, soak up blood with a PBS damp gauze. Expose chest, open rib cage and expose lungs, do not nick the lungs. Expose trachea, but do NOT severe the trachea. Free trachea from fascia gently with a fine tipped rounded forceps (eye forceps) then using a cuticle scissor (extremely fine tipped, look for the finest tip available) from WalMart or Target, place a tiny V shaped cut on top of trachea BUT DO NOT CUT ACROSS TRACHEA OR IT RETRACTS INTO CHEST AREA, ALL IS LOST! Use an 18 gauge needle dulled with a sandpaper style fingernail file, put it on a 5 ml syringe and fill syringe with OCT to 3 ml or use a 3 ml syringe filled to 2.5 ml (do not use any other cryomedia or you will have grief). Insert tip of needle gently into v shaped cut, push toward lung a tidge, then inject OCT into lung, watching them fill until just expanded, appear transluscent. Clamp off trachea with a mosquito forceps just below needle and as you pull out needle to prevent OCT leakage. DO NOT OVERFILL LUNGS with OCT OR you will blow up alveoli. Younger animals require smaller gauge needles and less OCT - have several needles dulled and ready to use if you can't get the needle inside the trachea with ease. If you inflate with more than 3 ml OCT with any mouse, you will damage lung. Dissect lung out with curved scissors, then remove heart with thymus. Snap freeze lung in Tissue Tek cryomold which we prefer for the thinner plastic. Use a petri dish supported with a metal tube rack inside a styrofoam box, and let dish FLOAT on Liquid nitrogen, do not put LiQ N2 in the dish, put cryomold with tissue in dish, let block freeze. Voila, perfect lung block for frozen sections, no more lousy frozen sections and no freezing artifact. One can use a dry ice/2 methyl butane or hexane mixture, but dry ice must be at top level of beaker and beaker surrounded with dry ice. This is more toxic and dangerous with explosive solvent, and bad to breathe - try the petri dish method. You must NOT crack the OCT or lung with crack too. For paraffin work, we fix lungs in the same way by perfusing with NBF via trachea. We used to use Cryojane, but this is far superior for our use these days. Cryojane we reserve for undecalcifed bone frozen sections. If the lung is deflated which happens when you euthanize the mouse, the Cryojane cannot correct that and you still get funky collapsed lung frozen sections. The natural inflated lung morphology is retained with OCT filling. We cut these sections at -20C using high profile disposable blades. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From lpwenk <@t> covad.net Thu Jan 8 04:42:21 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Bodian Stain References: <5.2.0.9.0.20040106142935.00a041e0@mailhost.vetmed.auburn.edu> Message-ID: <001a01c3d5d4$35405f20$58676940@hppav> I don't think Bodian would work in a microwave, as copper shot is used in the coplin jar. That's a metal, and I think it could cause arcing. I've done the Bodian, leaving it incubate overnight, such as putting it in at 4 pm and taking it out at 7-8 am. It works. Is there a way they could turn their drying oven down to the 37 degree C just for overnight? Or over the weekend? Below is our procedure. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 NERVE FIBERS - BODIAN PREPARED BY: Peggy A. Wenk, BS, HTL(ASCP)SLS ADOPTED BY: ____________________________________ DATE:___________________________ ADOPTED REVIEWED REVIEWED REVIEWED SUPERSEDES: PURPOSE: This stain demonstrates nerve fibers, nerve endings, and neurofibrils, which are aggregates of microtubules and neurofilaments found in the cell body, axon and dendrites of nerve cells. PRINCIPLE: The reaction is an argyrophilic silver stain. Protargol, a silver proteinate compound, impregnates the tissue sections. Copper, which is added to the incubating solution, is more reactive than the silver, and will remove the silver from the connective tissue. This allows for a greater differentiation between the neural fibers and the connective tissue. Hydroquinone and formaldehyde reduce the silver salts to visible metallic silver. Gold chloride tones the section. Oxalic acid is used to reduce the gold. This gives a darker stain, as the metallic gold is also deposited onto the tissue. Sodium thiosulfate removes excess unreduced silver. FIXATION: Any well fixed tissue. 10% neutral buffered formalin preferred. Avoid mercuric fixatives. TECHNIQUE: Cut routine paraffin sections at 8-10 um. CONTROL: Section of brain medulla or peripheral nerve. QUALITY CONTROL: 1. This is a capricious stain. Follow procedure exactly. Do NOT try to reduce percentages or time. 2. The quality of the silver proteinate seems to be critical to the success of this stain. EQUIPMENT: Balance, Erlenmeyer flasks, graduated cylinders, acid clean coplin jars, non-metal forceps, 37o C. oven. CAUTION: Follow standard safety procedures when preparing stains. SILVER PROTEINATE (PROTARGOL) is an irritant and is possibly toxic. HYDROQUINONE is an irritant to eyes, skin and respiratory system. FORMALDEHYDE is a poison. May be fatal or cause blindness if swallowed. Cannot be made non-poisonous. Possible cancer hazard. Irritating to eyes, skin and respiratory tract. Can cause severe eye burns. GOLD CHLORIDE is an irritant to eyes and skin. OXALIC ACID is a strong reducing agent. Contact with other material may cause fire. May cause skin and eye burns. Irritating to respiratory system. SODIUM THIOSULFATE is an irritant. REAGENTS: PROTARGOL (SILVER PROTEINATE) SOLUTION Protargol (silver proteinate) 0.5 g Distilled water 50.0 mL Place the distilled water in a 50 mL beaker. Sprinkle Protargol on the surface of the water. Do NOT shake or stir. Place in 37o C. oven for about 30 minutes, until it is dissolved. Make solution just before use. Discard after use. REDUCING SOLUTION Hydroquinone (C6H4(OH)2) 1.0 g Distilled water 50.0 mL Formaldehyde, 37-40% (HCHO) 2.5 mL JUST BEFORE USE, dissolve together hydroquinone in distilled water. Add formaldehyde. Discard after use. 1% GOLD CHLORIDE Gold chloride (HAuCl4C3H2O) 1.0 g Distilled water 100.0 mL Dissolve together. Store at room temperature. Stable for months. May be reused. Filter when necessary. 2% OXALIC ACID Oxalic acid ((COOH)2C2H2O) 2.0 g Distilled water 100.0 mL Dissolve together. Store at room temperature. Stable for months. 5% SODIUM THIOSULFATE Sodium thiosulfate (Na2S2O3) 5.0 g Distilled water 100.0 mL Dissolve together. Store at room temperature. Stable for months. PROCEDURE - Bodian: 1. Deparaffinize and hydrate slides through graded alcohol to distilled water. 2. Place 3 g copper shot in an acid-clean coplin jar. 3. Pour protargol solution over copper shots. 4. Place slides in protargol solution. 5. Incubate slides in 37o C. oven 24-72 hours 6. Rinse in 3 changes of distilled water 5 seconds each 7. Place in reducing solution 10 minutes 8. Rinse in distilled water, 3 changes 5-10 seconds each 9. Tone in 1% gold chloride 5 minutes 10. Rinse in distilled water, 3 changes 5-10 seconds each 11. Develop in 2% oxalic acid until background is gray and nerve fibers appear clearly black 3-5 minutes 12. Rinse in distilled water, 3 changes 5-10 seconds each 13. Place in 5% sodium thiosulfate 5 minutes 14. Wash in running water 10 minutes 15. Dehydrate through graded alcohols and clear in xylene. 16. Coverslip with a synthetic mounting media. RESULTS: Nerve fibers - black Connective tissue - gray to black Background - gray/purple PROCEDURAL NOTES: 1. If copper shots appear "rusty", clean them by placing in a solution of aqua regia (15 mL hydrochloric acid, concentrated, and 5 mL nitric acid, concentrated). Use gloves and apron when preparing or handling this solution. Prepare and use under hood. Wash copper shots in running water, and then distilled water, before using in protargol solution. 2. Leave the Protargol to dissolve from the surface downward. Do not disturb until dissolved. Do not allow to coagulate. 3. Prolonged treatment in oxalic acid will destroy the protargol reaction. 4. Use acid-cleaned coplin jars and non-metal forceps, or a dirty background may appear. 5. Nuclear fast red may be used as a counterstain. Nuclei will be stained pink. 6. Use 1% gold chloride, not the more dilute used for most silver stain toning. Tone for entire 5 minutes. Using lower percentage gold chloride or less time will cause the nerves and background not to turn to the characteristic Bodian purple-black. REFERENCES: Bancroft JD, Stevens A: Theory and Practice of Histological Techniques, 3rd ed. New York, NY, Churchill Livingstone, 1990. Carson FL: Histotechnology: A Self-Instructional Text, Chicago, IL, ASCP Press, 1990. Sheehan DC, Hrapchak BB: Theory and Practice of Histotechnology, 2nd edition. Columbus, Ohio, Battelle Press, 1980. Vacca LL: Laboratory Manual of Histochemistry, New York, New York, Raven Press, 1985. * Actual source of procedure is unknown. ----- Original Message ----- From: "Atoska S. Gentry" To: "Histonet" Sent: Tuesday, January 06, 2004 3:42 PM Subject: [Histonet] Bodian Stain > Hello, a former colleague of mine who works in the only local human > pathology lab is seeking input on the availability of a protocol for Bodian > Stain other than the one in the AFIP manual. The reason they are seeking > this is their lab is only equipped with one incubator and they routinely > use it for drying slides at temperatures much higher than the required > 48hour, 37C incubation of the protargol. They are equipped with a microwave > for staining but are not sure how to use it for drying of slides without > trial and error. Any suggestions provided will be much appreciated. Thanks, > Atoska p.s. our lab is equipped but we only process animal tissues here. > We've provided them with the chemicals they were lacking to complete this > stain. > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Thu Jan 8 05:02:57 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Creating an HT from a BS References: Message-ID: <005401c3d5d6$f99adf60$58676940@hppav> For HT, after the Jan. 1, 2005 deadline, there will still be 2 routes 1. Completion of a NAACLS accredited HT program. (high school diploma through any amount of college is still OK). 2. Associate degree with 20 credits biology/chemistry (combined) plus 1 year on-the-job (OJT) training. OJT for histology means they have worked with tissue, and have fixed, processed, embedded, sectioned, stained, and did lab math and safety and made solutions. So someone who was just a lab assistant would not be qualified to take it, if all they did was computer work, run the H&E machine, labeled slides and cleaned up. Microtomy and embedding must be included. However, if the person worked as a tech only in the IHC lab, or only in EM, they could take the exam, because they are cutting and staining, etc. The ASCP BOR requirements does NOT state that it has to be paraffin only, or histology stains only. But they would still have to know all about the histology procedures to pass the exam, and they would still have to do the practical exam (though if they could do it well on methacrylate embedded tissues, these would be acceptable). So if your person has the BS in biology with at least 20 credits in biology and chemistry combined, she can take the HT exam once she gets 1 year full time histology experience. If this person has the BS with 30 credit hours of biology/chemistry combined, plus the 1 year OJT (same criteria), she can take the HTL exam. The only one who cannot take the HT exam after Jan. 1, 2005 would be someone with less than the associate degree + 20 credits biology/chemistry + 1 yr OJT. So the high school graduate or the person with only a couple college courses would not be able to take the HT exam, if they didn't first take the HT exam before Jan. 1, 2005. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Dawson, Glen" To: Sent: Wednesday, January 07, 2004 1:08 PM Subject: [Histonet] Creating an HT from a BS > All, > > I know we've talked about this topic many times but I have a specific case I > would appreciate some help with. There is a lab helper who has been > performing various types of labwork for years and has been working in > histology now for a couple of months. She has a BS in biology and I would > like to help her become a registered histotech. My hope is that, with so > much previous lab experience as well as the bachelor's degree under her > belt, she can sneak in under the bench-trained tech category before the 2005 > deadline. Taking an accredited HT program is not a possibility for her as > she would need to relocate to a place that offers a program which she could > not currently afford anyway. Could anyone give me an idea about the > feasibility of this idea? > > Thank-you in Advance, > > Glen Dawson BS, HT & QIHC (ASCP) > Lead IHC Technologist > Milwaukee, WI > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hamilt_l <@t> hotmail.com Thu Jan 8 06:33:05 2004 From: hamilt_l <@t> hotmail.com (Loriann Hamilton) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Tissue Separating from paraffin ribbon Message-ID: Hello, We are experiencing our animal tissue separating and rolling back on itself at sectioning. We currently use Kendall's Paraplast plus and we infiltrate in two changes of paraffin at processing each being two hours. We use the open auto technicon processors. The processors were changed prior to processing so we do not believe the paraffin was dirty. Any insight would be greatly appreciated. Thank you, Loriann _________________________________________________________________ Make your home warm and cozy this winter with tips from MSN House & Home. http://special.msn.com/home/warmhome.armx From Tracey.Smith <@t> childrenshc.org Thu Jan 8 08:12:39 2004 From: Tracey.Smith <@t> childrenshc.org (Tracey Smith) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Iron stain Message-ID: <18378247.1073571176386.JavaMail.SYSTEM@CPMAIL1> Rebecca, in most labs I've worked in, we use a Pearle's Prussian Blue method for all our Iron stains regardless of tissue or blood smear. You do need to fix the smear in Acetone free methanol for fifteen minutes first. If you are looking for siderocytes, then the preferred method would be a Dacie stain with fixing the smear first in 37% formaldehyde fumes for at least five minutes. Since I am now at home I do not have the methods handy but I would guess most good staining books would have the procedures in them. Signing off from the frozen tundras of Minnesota. Tracy Smith(proud member of SOW'96) >>> "Rebecca Barnhart" 01/07/04 01:36PM >>> What stain does anyone use for bone marrow aspirate, blood smears and tissue. Also how long does it take to do the entire stain. We purchased a kit and when we try to stain a fixed blood smear everything is washed away. Thanks in advance. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Tracy Smith Anatomic Pathology Scientist Children's Hospital and Clinics Phone(651)220-6561 Fax(651)220-7178 Tracy.Smith@Children'sHC.org __________________________________ Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From katherine-walters <@t> uiowa.edu Thu Jan 8 08:14:21 2004 From: katherine-walters <@t> uiowa.edu (katherine-walters@uiowa.edu) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] IMEB service contract Message-ID: <1073571261.3ffd65bdae489@webmail1.its.uiowa.edu> Hi, We are considering putting our 4 Microm Cryostats on service contract with IMEB. I am not familiar with their service or turn-around time. Has anyone had service experiences with IMEB that they would be willing to share? Thanks for any information. Kathy Walters From RITA.ANGEL <@t> UC.EDU Thu Jan 8 08:57:21 2004 From: RITA.ANGEL <@t> UC.EDU (Rita Angel) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] mouse lungs Message-ID: <5.1.0.14.2.20040108095420.00b56550@ucmail3.uc.edu> Thanks to everyone that responded to my question about freezing mouse lungs. It turns out the investigator was inflating the lungs with a dilution of pbs/oct. So maybe now we need to try the full-strength oct. Thanks again for sharing your experience and expertise, Rita Angel, HT University of Cincinnati From siksik03 <@t> comcast.net Thu Jan 8 09:05:13 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Bodian Stain In-Reply-To: <001a01c3d5d4$35405f20$58676940@hppav> References: <5.2.0.9.0.20040106142935.00a041e0@mailhost.vetmed.auburn.edu> <001a01c3d5d4$35405f20$58676940@hppav> Message-ID: Hi HistoNetters Actually, there is a very good microwave Bodian stain. It is procedure 15.23 in the new edition of Kok & Boon's book (pp 219-220). The staining time is reduced to 2-1/2 hours. There will be no arcing if no metallic accessories are used. best regards, Steven Slap Microwave Consultant At 5:42 AM -0500 1/8/04, Lee & Peggy Wenk wrote: >I don't think Bodian would work in a microwave, as copper shot is used in >the coplin jar. That's a metal, and I think it could cause arcing. > >I've done the Bodian, leaving it incubate overnight, such as putting it in >at 4 pm and taking it out at 7-8 am. It works. > >Is there a way they could turn their drying oven down to the 37 degree C >just for overnight? Or over the weekend? > >Below is our procedure. > >Peggy A. Wenk, HTL(ASCP)SLS >William Beaumont Hospital >Royal Oak, MI 48073 > > >NERVE FIBERS - BODIAN > >PREPARED BY: Peggy A. Wenk, BS, HTL(ASCP)SLS > >ADOPTED BY: ____________________________________ >DATE:___________________________ > > ADOPTED >REVIEWED >REVIEWED >REVIEWED > >SUPERSEDES: > >PURPOSE: >This stain demonstrates nerve fibers, nerve endings, and neurofibrils, which >are aggregates of microtubules and neurofilaments found in the cell body, >axon and dendrites of nerve cells. > >PRINCIPLE: >The reaction is an argyrophilic silver stain. Protargol, a silver proteinate >compound, impregnates the tissue sections. Copper, which is added to the >incubating solution, is more reactive than the silver, and will remove the >silver from the connective tissue. This allows for a greater differentiation >between the neural fibers and the connective tissue. Hydroquinone and >formaldehyde reduce the silver salts to visible metallic silver. Gold >chloride tones the section. Oxalic acid is used to reduce the gold. This >gives a darker stain, as the metallic gold is also deposited onto the >tissue. Sodium thiosulfate removes excess unreduced silver. > >FIXATION: >Any well fixed tissue. >10% neutral buffered formalin preferred. >Avoid mercuric fixatives. > >TECHNIQUE: >Cut routine paraffin sections at 8-10 um. > >CONTROL: >Section of brain medulla or peripheral nerve. > >QUALITY CONTROL: >1. This is a capricious stain. Follow procedure exactly. Do NOT try to >reduce percentages or time. >2. The quality of the silver proteinate seems to be critical to the success >of this stain. > >EQUIPMENT: >Balance, Erlenmeyer flasks, graduated cylinders, acid clean coplin jars, >non-metal forceps, 37o C. oven. > > CAUTION: >Follow standard safety procedures when preparing stains. > >SILVER PROTEINATE (PROTARGOL) is an irritant and is possibly toxic. >HYDROQUINONE is an irritant to eyes, skin and respiratory system. >FORMALDEHYDE is a poison. May be fatal or cause blindness if swallowed. >Cannot be made > non-poisonous. Possible cancer hazard. Irritating to eyes, skin and >respiratory tract. > Can cause severe eye burns. >GOLD CHLORIDE is an irritant to eyes and skin. >OXALIC ACID is a strong reducing agent. Contact with other material may >cause fire. May > cause skin and eye burns. Irritating to respiratory system. >SODIUM THIOSULFATE is an irritant. > >REAGENTS: >PROTARGOL (SILVER PROTEINATE) SOLUTION >Protargol (silver proteinate) 0.5 g >Distilled water 50.0 mL >Place the distilled water in a 50 mL beaker. Sprinkle Protargol on the >surface of the water. Do NOT shake or stir. Place in 37o C. oven for about >30 minutes, until it is dissolved. Make solution just before use. Discard >after use. > >REDUCING SOLUTION >Hydroquinone (C6H4(OH)2) 1.0 g >Distilled water 50.0 mL >Formaldehyde, 37-40% (HCHO) 2.5 mL >JUST BEFORE USE, dissolve together hydroquinone in distilled water. Add >formaldehyde. Discard after use. > >1% GOLD CHLORIDE >Gold chloride (HAuCl4C3H2O) 1.0 g >Distilled water 100.0 mL >Dissolve together. Store at room temperature. Stable for months. May be >reused. Filter when necessary. > >2% OXALIC ACID >Oxalic acid ((COOH)2C2H2O) 2.0 g >Distilled water 100.0 mL >Dissolve together. Store at room temperature. Stable for months. > >5% SODIUM THIOSULFATE >Sodium thiosulfate (Na2S2O3) 5.0 g >Distilled water 100.0 mL >Dissolve together. Store at room temperature. Stable for months. > > >PROCEDURE - Bodian: >1. Deparaffinize and hydrate slides through graded alcohol to distilled >water. > >2. Place 3 g copper shot in an acid-clean coplin jar. > >3. Pour protargol solution over copper shots. > >4. Place slides in protargol solution. > >5. Incubate slides in 37o C. oven 24-72 hours > >6. Rinse in 3 changes of distilled water 5 seconds each > >7. Place in reducing solution 10 minutes > >8. Rinse in distilled water, 3 changes 5-10 seconds each > >9. Tone in 1% gold chloride 5 minutes > >10. Rinse in distilled water, 3 changes 5-10 seconds each > >11. Develop in 2% oxalic acid until background is gray >and nerve fibers appear clearly black 3-5 minutes > >12. Rinse in distilled water, 3 changes 5-10 seconds each > >13. Place in 5% sodium thiosulfate 5 minutes > >14. Wash in running water 10 minutes > >15. Dehydrate through graded alcohols and clear in xylene. > >16. Coverslip with a synthetic mounting media. > > >RESULTS: >Nerve fibers - black >Connective tissue - gray to black >Background - gray/purple > > >PROCEDURAL NOTES: >1. If copper shots appear "rusty", clean them by placing in a solution of >aqua regia (15 mL hydrochloric acid, concentrated, and 5 mL nitric acid, >concentrated). Use gloves and apron when preparing or handling this >solution. Prepare and use under hood. Wash copper shots in running water, >and then distilled water, before using in protargol solution. > >2. Leave the Protargol to dissolve from the surface downward. Do not disturb >until dissolved. Do not allow to coagulate. > >3. Prolonged treatment in oxalic acid will destroy the protargol reaction. > >4. Use acid-cleaned coplin jars and non-metal forceps, or a dirty background >may appear. > >5. Nuclear fast red may be used as a counterstain. Nuclei will be stained >pink. > >6. Use 1% gold chloride, not the more dilute used for most silver stain >toning. Tone for entire 5 minutes. Using lower percentage gold chloride or >less time will cause the nerves and background not to turn to the >characteristic Bodian purple-black. > > >REFERENCES: > >Bancroft JD, Stevens A: Theory and Practice of Histological Techniques, 3rd >ed. New York, NY, Churchill Livingstone, 1990. > >Carson FL: Histotechnology: A Self-Instructional Text, Chicago, IL, ASCP >Press, 1990. > >Sheehan DC, Hrapchak BB: Theory and Practice of Histotechnology, 2nd >edition. Columbus, Ohio, Battelle Press, 1980. > >Vacca LL: Laboratory Manual of Histochemistry, New York, New York, Raven >Press, 1985. > >* Actual source of procedure is unknown. > > > >----- Original Message ----- >From: "Atoska S. Gentry" >To: "Histonet" >Sent: Tuesday, January 06, 2004 3:42 PM >Subject: [Histonet] Bodian Stain > > >> Hello, a former colleague of mine who works in the only local human >> pathology lab is seeking input on the availability of a protocol for >Bodian >> Stain other than the one in the AFIP manual. The reason they are seeking >> this is their lab is only equipped with one incubator and they routinely >> use it for drying slides at temperatures much higher than the required >> 48hour, 37C incubation of the protargol. They are equipped with a >microwave >> for staining but are not sure how to use it for drying of slides without >> trial and error. Any suggestions provided will be much appreciated. >Thanks, >> Atoska p.s. our lab is equipped but we only process animal tissues here. >> We've provided them with the chemicals they were lacking to complete this >> stain. >> >> Atoska S. Gentry B.S., HT(ASCP) >> Research Assistant III >> Scott-Ritchey Research Center >> College of Veterinary Medicine >> Auburn University, AL 36849 >> Phone# (334)844-5579 Fax# (334)844-5850 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peggy <@t> nsh.org Thu Jan 8 09:28:00 2004 From: peggy <@t> nsh.org (Peggy Micciche) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] BS to Histotech can be done without moving! In-Reply-To: <3.0.6.32.20040107163346.00bc4c08@gemini.msu.montana.edu> Message-ID: <000001c3d5fc$006c2c70$7300000a@histo2> In addition to Glenda's program there is another program similiar to Glenda's at Harford Community College in Maryland. Lectures are online with completion of lab work at one's home lab. Contact the program director, Floyd Grimm at fgrimm@harford.edu. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Wednesday, January 07, 2004 6:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BS to Histotech can be done without moving! You Wrote: I know we've talked about this topic many times but I have a specific case I would appreciate some help with. There is a lab helper who has been performing various types of labwork for years and has been working in histology now for a couple of months. She has a BS in biology and I would like to help her become a registered histotech. My hope is that, with so much previous lab experience as well as the bachelor's degree under her belt, she can sneak in under the bench-trained tech category before the 2005 deadline. Taking an accredited HT program is not a possibility for her as she would need to relocate to a place that offers a program which she could not currently afford anyway. Could anyone give me an idea about the feasibility of this idea? Thank-you in Advance, **************************************************************** She can enroll in a histotech program while working in your lab, it is excellent and well worth the effort, time and money. Contact Glenda Hoye at Indiana University (ghoye@iupui.edu) none of her students are on site at the university but work under the auspices of a trained HT at their labs. What a wonderful way to become a registered histotech if one cannot relocate and there may be other programs available and comparable to Glenda's. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jan 8 09:26:52 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Bodian Stain In-Reply-To: <001a01c3d5d4$35405f20$58676940@hppav> References: <5.2.0.9.0.20040106142935.00a041e0@mailhost.vetmed.auburn.edu> Message-ID: <3.0.6.32.20040108082652.00bd7088@gemini.msu.montana.edu> How about calibrating a waterbath to 37C and using that, avoiding a convectional drying oven. Once the waterbath is calibrated, then you know settings for next go 'round of Bodian. Waterbath heating is superior to drying ovens as the heating is consistently even. At 05:42 AM 1/8/2004 -0500, you wrote: >I don't think Bodian would work in a microwave, as copper shot is used in >the coplin jar. That's a metal, and I think it could cause arcing. > >I've done the Bodian, leaving it incubate overnight, such as putting it in >at 4 pm and taking it out at 7-8 am. It works. > >Is there a way they could turn their drying oven down to the 37 degree C >just for overnight? Or over the weekend? > >Below is our procedure. > >Peggy A. Wenk, HTL(ASCP)SLS >William Beaumont Hospital >Royal Oak, MI 48073 > > >NERVE FIBERS - BODIAN > >PREPARED BY: Peggy A. Wenk, BS, HTL(ASCP)SLS > >ADOPTED BY: ____________________________________ >DATE:___________________________ > > ADOPTED >REVIEWED >REVIEWED >REVIEWED > >SUPERSEDES: > >PURPOSE: >This stain demonstrates nerve fibers, nerve endings, and neurofibrils, which >are aggregates of microtubules and neurofilaments found in the cell body, >axon and dendrites of nerve cells. > >PRINCIPLE: >The reaction is an argyrophilic silver stain. Protargol, a silver proteinate >compound, impregnates the tissue sections. Copper, which is added to the >incubating solution, is more reactive than the silver, and will remove the >silver from the connective tissue. This allows for a greater differentiation >between the neural fibers and the connective tissue. Hydroquinone and >formaldehyde reduce the silver salts to visible metallic silver. Gold >chloride tones the section. Oxalic acid is used to reduce the gold. This >gives a darker stain, as the metallic gold is also deposited onto the >tissue. Sodium thiosulfate removes excess unreduced silver. > >FIXATION: >Any well fixed tissue. >10% neutral buffered formalin preferred. >Avoid mercuric fixatives. > >TECHNIQUE: >Cut routine paraffin sections at 8-10 um. > >CONTROL: >Section of brain medulla or peripheral nerve. > >QUALITY CONTROL: >1. This is a capricious stain. Follow procedure exactly. Do NOT try to >reduce percentages or time. >2. The quality of the silver proteinate seems to be critical to the success >of this stain. > >EQUIPMENT: >Balance, Erlenmeyer flasks, graduated cylinders, acid clean coplin jars, >non-metal forceps, 37o C. oven. > > CAUTION: >Follow standard safety procedures when preparing stains. > >SILVER PROTEINATE (PROTARGOL) is an irritant and is possibly toxic. >HYDROQUINONE is an irritant to eyes, skin and respiratory system. >FORMALDEHYDE is a poison. May be fatal or cause blindness if swallowed. >Cannot be made > non-poisonous. Possible cancer hazard. Irritating to eyes, skin and >respiratory tract. > Can cause severe eye burns. >GOLD CHLORIDE is an irritant to eyes and skin. >OXALIC ACID is a strong reducing agent. Contact with other material may >cause fire. May > cause skin and eye burns. Irritating to respiratory system. >SODIUM THIOSULFATE is an irritant. > >REAGENTS: >PROTARGOL (SILVER PROTEINATE) SOLUTION >Protargol (silver proteinate) 0.5 g >Distilled water 50.0 mL >Place the distilled water in a 50 mL beaker. Sprinkle Protargol on the >surface of the water. Do NOT shake or stir. Place in 37o C. oven for about >30 minutes, until it is dissolved. Make solution just before use. Discard >after use. > >REDUCING SOLUTION >Hydroquinone (C6H4(OH)2) 1.0 g >Distilled water 50.0 mL >Formaldehyde, 37-40% (HCHO) 2.5 mL >JUST BEFORE USE, dissolve together hydroquinone in distilled water. Add >formaldehyde. Discard after use. > >1% GOLD CHLORIDE >Gold chloride (HAuCl4C3H2O) 1.0 g >Distilled water 100.0 mL >Dissolve together. Store at room temperature. Stable for months. May be >reused. Filter when necessary. > >2% OXALIC ACID >Oxalic acid ((COOH)2C2H2O) 2.0 g >Distilled water 100.0 mL >Dissolve together. Store at room temperature. Stable for months. > >5% SODIUM THIOSULFATE >Sodium thiosulfate (Na2S2O3) 5.0 g >Distilled water 100.0 mL >Dissolve together. Store at room temperature. Stable for months. > > >PROCEDURE - Bodian: >1. Deparaffinize and hydrate slides through graded alcohol to distilled >water. > >2. Place 3 g copper shot in an acid-clean coplin jar. > >3. Pour protargol solution over copper shots. > >4. Place slides in protargol solution. > >5. Incubate slides in 37o C. oven 24-72 hours > >6. Rinse in 3 changes of distilled water 5 seconds each > >7. Place in reducing solution 10 minutes > >8. Rinse in distilled water, 3 changes 5-10 seconds each > >9. Tone in 1% gold chloride 5 minutes > >10. Rinse in distilled water, 3 changes 5-10 seconds each > >11. Develop in 2% oxalic acid until background is gray >and nerve fibers appear clearly black 3-5 minutes > >12. Rinse in distilled water, 3 changes 5-10 seconds each > >13. Place in 5% sodium thiosulfate 5 minutes > >14. Wash in running water 10 minutes > >15. Dehydrate through graded alcohols and clear in xylene. > >16. Coverslip with a synthetic mounting media. > > >RESULTS: >Nerve fibers - black >Connective tissue - gray to black >Background - gray/purple > > >PROCEDURAL NOTES: >1. If copper shots appear "rusty", clean them by placing in a solution of >aqua regia (15 mL hydrochloric acid, concentrated, and 5 mL nitric acid, >concentrated). Use gloves and apron when preparing or handling this >solution. Prepare and use under hood. Wash copper shots in running water, >and then distilled water, before using in protargol solution. > >2. Leave the Protargol to dissolve from the surface downward. Do not disturb >until dissolved. Do not allow to coagulate. > >3. Prolonged treatment in oxalic acid will destroy the protargol reaction. > >4. Use acid-cleaned coplin jars and non-metal forceps, or a dirty background >may appear. > >5. Nuclear fast red may be used as a counterstain. Nuclei will be stained >pink. > >6. Use 1% gold chloride, not the more dilute used for most silver stain >toning. Tone for entire 5 minutes. Using lower percentage gold chloride or >less time will cause the nerves and background not to turn to the >characteristic Bodian purple-black. > > >REFERENCES: > >Bancroft JD, Stevens A: Theory and Practice of Histological Techniques, 3rd >ed. New York, NY, Churchill Livingstone, 1990. > >Carson FL: Histotechnology: A Self-Instructional Text, Chicago, IL, ASCP >Press, 1990. > >Sheehan DC, Hrapchak BB: Theory and Practice of Histotechnology, 2nd >edition. Columbus, Ohio, Battelle Press, 1980. > >Vacca LL: Laboratory Manual of Histochemistry, New York, New York, Raven >Press, 1985. > >* Actual source of procedure is unknown. > > > >----- Original Message ----- >From: "Atoska S. Gentry" >To: "Histonet" >Sent: Tuesday, January 06, 2004 3:42 PM >Subject: [Histonet] Bodian Stain > > >> Hello, a former colleague of mine who works in the only local human >> pathology lab is seeking input on the availability of a protocol for >Bodian >> Stain other than the one in the AFIP manual. The reason they are seeking >> this is their lab is only equipped with one incubator and they routinely >> use it for drying slides at temperatures much higher than the required >> 48hour, 37C incubation of the protargol. They are equipped with a >microwave >> for staining but are not sure how to use it for drying of slides without >> trial and error. Any suggestions provided will be much appreciated. >Thanks, >> Atoska p.s. our lab is equipped but we only process animal tissues here. >> We've provided them with the chemicals they were lacking to complete this >> stain. >> >> Atoska S. Gentry B.S., HT(ASCP) >> Research Assistant III >> Scott-Ritchey Research Center >> College of Veterinary Medicine >> Auburn University, AL 36849 >> Phone# (334)844-5579 Fax# (334)844-5850 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Jan 8 09:32:27 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Tissue Separating from paraffin ribbon In-Reply-To: Message-ID: <3.0.6.32.20040108083227.00bd7088@gemini.msu.montana.edu> You didn't say how you fixed, time, what animal or processing schedule and if you use vacuum/pressure, etc. 2 hours per change of paraffin may be excessive for lean aka less fatty animal tissues. Dirty paraffin is more likely to cause ski tracks in the sections. Do you ice water soak your tissues after trimming for a short time? Check your paraplast plus, we used to get water droplets in bottom of this paraffin after melting, strange - even though we stored the paraffin properly. At 07:33 AM 1/8/2004 -0500, you wrote: >Hello, > >We are experiencing our animal tissue separating and rolling back on itself >at sectioning. >We currently use Kendall's Paraplast plus and we infiltrate in two changes >of paraffin at processing each being two hours. We use the open auto >technicon processors. The processors were changed prior to processing so we >do not believe the paraffin was dirty. Any insight would be greatly >appreciated. > >Thank you, >Loriann > >_________________________________________________________________ >Make your home warm and cozy this winter with tips from MSN House & Home. >http://special.msn.com/home/warmhome.armx > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Jan 8 09:42:11 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] mouse lungs In-Reply-To: <5.1.0.14.2.20040108095420.00b56550@ucmail3.uc.edu> Message-ID: <3.0.6.32.20040108084211.00bd7088@gemini.msu.montana.edu> If you dilute OCT with PBS and inflate lungs, beware - this tends to leak out of lung, and you have to work very fast to snap freeze. Sometime the dilution is too great e.g. 1 part OCT and 2 parts water, you introduce more water than you want, which makes cutting difficult. The lungs will inflate as well with full strength OCT as long as you use the 18 gauge needle to inject it. Also, do you use the ORIGINAL OCT from Sakura Finetek aka Miles OCT? Some cryomedias (we learned this the hard way) don't seem to work as well with inflated mouse lungs and intestine - and we found out one was tested with human tissue, and not with mouse nor special application we needed - inflated lung and small intestine. Sectioning can be difficult and section quality poor. This does not mean any given cryomedia is defective, but more suited to clinical or other applications. Try them out! There are a bunch out on the market. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Annette_hall <@t> pa-ucl.com Thu Jan 8 10:13:59 2004 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Iron Stain on Bone Marrow Smears Message-ID: <21EE03EF1DB9D511B112000255FC299486E1DC@mercury.pa-ucl.com> Hello Histonetters, One of our pathologists does not like the quality of the bone marrow smears when stained on the Artisan (DakoCytomation). He describes the cells as having "blown out nuclei". 9 months ago he saw this same problem. We decreased the incubation temperature from 37 to 23 degrees. It seemed to improve the stain somewhat. However the last 2 or 3 cases of his have again been unacceptable. The protocol on the Artisan is relatively simple. (see below) Has anyone else seen this or have an idea on what we can change to troubleshoot? Thanks for your help in advance. Annette J Hall, MT United Clinical Labs 205 Bluff St. Dubuque, IA 52001 Iron for Bone Marrow Smears Potassium ferrocyanide 1 ml and HCL 1 ml Incubate 260 seconds at 23 deg. Mix 4x Wash 4x Nuclear Fast Red 1 ml. Incubate 280 sec at 23 deg Mix 1x Wash 6x. From james.zimmerman <@t> pharma.novartis.com Thu Jan 8 11:04:47 2004 From: james.zimmerman <@t> pharma.novartis.com (james.zimmerman@pharma.novartis.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] (no subject) Message-ID: Hello Everyone, Does anyone have a procedure and vendor for CD15 for use in primates and suitable for use in formalin fixed,parrafin embedded tissue? All help will be greatly appreciated ! Thanks, JPZ From DDittus787 <@t> aol.com Thu Jan 8 11:52:42 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Iron Stain on Bone Marrow Smears Message-ID: <12E7DD3F.635B37BD.0A1F969F@aol.com> blown out nuclei iss not a stain problem , but has been a problem in our lab from temp and believe it or not exhausted xylene, i say this because you will notice that this is 1. an intermitent problem 2. may occur (as it did with us) on any number of different stains 3. tends to show up later in the week(when reagents are getting older) my suggestion to you is to change your depapraffinizing station mor often or use only fresh reagents for bone marrows and see if it still occurs. we now change our xylenes more often and did reduce temp and we very rarely if almost never see this problem. good luck dana From shive003 <@t> umn.edu Thu Jan 8 11:56:01 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] anti-CDV, Neospora and TGE Message-ID: <00bf01c3d610$ad41b050$78065486@vdl220FAC> For you animal IHC people out there: I've just been informed that my current supplier of anti-Canine Distemper Virus monoclonal antibody is discontinuing that product. Can anyone help me out with information on another source? I also am in need of antibodies to Transmissible Gastroenteritis Virus (TGE; swine coronavirus) and Neospora. I'm nearly out of my private stock and need to find a commercial source. Monoclonals would be preferrable. All have to be unconjugated and all have to work on FFPE tissue with the IHC technique. Thank you in advance. Jan Shivers U of MN Vet Diag Lab From rfail <@t> toolkitmail.com Thu Jan 8 12:17:08 2004 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Jim Burchette's e-mail address Message-ID: <3ffd9ea4.1a.5668.1531024@toolkitmail.com> Jim, I never seem to be able to find your e-mail address here at work. Would you please send it to me again. Thanks Rena From info <@t> instrumedics.com Thu Jan 8 12:25:51 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Lung frozens References: Message-ID: <01d801c3d614$d8c98000$6501a8c0@INSTRUMEDICS22> Lung frozen sections with the morphology preserved are not easy to produce. Many labs find that by snap-freezing the lung(Gentle Jane or other means) and using the CryoJane Tape-Transfer system they are able to prepare flat, intact sections of good quality. Bernice schiler@instrumedics.com Message ----- From: To: Sent: Thursday, January 08, 2004 1:00 PM Subject: Histonet Digest, Vol 2, Issue 7 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > ---------------------------------------------------------------------------- ---- > Today's Topics: > > 1. Creating an HT from a BS (Dawson, Glen) > 2. Iron stain (Rebecca Barnhart) > 3. autofluorescence in thraustochytrids (protists) > (McCollough, Carol) > 4. RE: Creating an HT from a BS (Horn, Hazel V) > 5. Protargol (MTitford@aol.com) > 6. Re: Creating an HT from a BS (Gareth Davis) > 7. BS to Histotech can be done without moving! (Gayle Callis) > 8. Freezing mouse lungs (Gayle Callis) > 9. Re: Bodian Stain (Lee & Peggy Wenk) > 10. Re: Creating an HT from a BS (Lee & Peggy Wenk) > 11. Tissue Separating from paraffin ribbon (Loriann Hamilton) > 12. Re: Iron stain (Tracey Smith) > 13. IMEB service contract (katherine-walters@uiowa.edu) > 14. mouse lungs (Rita Angel) > 15. Re: Bodian Stain (Steven E. Slap) > 16. RE: BS to Histotech can be done without moving! (Peggy Micciche) > 17. Re: Bodian Stain (Gayle Callis) > 18. Re: Tissue Separating from paraffin ribbon (Gayle Callis) > 19. Re: mouse lungs (Gayle Callis) > 20. Iron Stain on Bone Marrow Smears (Annette Hall) > 21. (no subject) (james.zimmerman@pharma.novartis.com) > 22. Re: Iron Stain on Bone Marrow Smears (DDittus787@aol.com) > 23. anti-CDV, Neospora and TGE (Jan Shivers) > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kstoll <@t> stjosephswb.com Thu Jan 8 13:58:02 2004 From: kstoll <@t> stjosephswb.com (Stoll, Kathy) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Steiners problems-milky staining on pt tissue Message-ID: Our lab has been having problems with our Steiners stain for the past several months. Our control slide usually turns out good, but the patient slide has milky grey coloring under the scope. Grossly the patient tissue has a gold coating. Occasionally both the patient and control slides have background staining, but not always. We use the following 10% NBF, control slides cut 2-3 months ago. We have tried the following...different batch of slides, making new solutions (we mix our own), acid cleaning the patient slide, glass coplin jars instead of plastic, different temperatures primarily for reducing solution, bottled water instead of tap distilled. This problem is recent and one that we had never had before. Thank you in advance for your help in solving our mystery. Kathy Stoll, HT St. Joseph's Community Hospital West Bend WI ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com ********************************************************************** From JWEEMS <@t> sjha.org Thu Jan 8 14:07:29 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Steiners problems-milky staining on pt tissue Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44B6D8E9@exch4.sjha.org> Have you had any B5 fixative anywhere - tissue processed in the same processor? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stoll, Kathy Sent: Thursday, January 08, 2004 2:58 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Steiners problems-milky staining on pt tissue Our lab has been having problems with our Steiners stain for the past several months. Our control slide usually turns out good, but the patient slide has milky grey coloring under the scope. Grossly the patient tissue has a gold coating. Occasionally both the patient and control slides have background staining, but not always. We use the following 10% NBF, control slides cut 2-3 months ago. We have tried the following...different batch of slides, making new solutions (we mix our own), acid cleaning the patient slide, glass coplin jars instead of plastic, different temperatures primarily for reducing solution, bottled water instead of tap distilled. This problem is recent and one that we had never had before. Thank you in advance for your help in solving our mystery. Kathy Stoll, HT St. Joseph's Community Hospital West Bend WI ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Dndsomi <@t> aol.com Thu Jan 8 14:23:01 2004 From: Dndsomi <@t> aol.com (Dndsomi@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Special Stain Kits Message-ID: <12e.39168da0.2d2f1625@aol.com> Does anyone out there in histoland use any of the Surgipath special stain kits? Thanks for your help ! DDietz From pruegg <@t> colobio.com Thu Jan 8 14:24:42 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] mouse bile duct epithelium Message-ID: here is my story: i am trying to label ms bile duct epithelium with mouse anti-mouse cytokeratin 7 and 19 (sounds odd, but that is what the investigator tells me it is, not even rat anti-mouse) they tried the anti-human ck7&19 and it did not cross react with the mouse. I have the reagents to biotinylate these ms colobio.com Thu Jan 8 14:39:17 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] frozen heart again Message-ID: I have pieces of frozen human heart that was not optomally frozen (meaning they just put it in a -70 freezer without cryo protectant OCT or anything). I cut off a piece while it was still frozen and mounted some in OCT and made frozen sections. As you can imagine the sections are not good. The muscle is shredded looking with lots of spaces and unrecognizable as muscle. There are cells which could be myocytes but who knows. I did some IHC on them and definately have nuclear envelope staining on some cells. I am labeling with Lap2 and Lamin A&C. I also tried Ki-67 and CD117-cKit, these were both completely negative (good neg. control I guess). Negatives were negative. Is it possible to reserrect this frozen tissue or will it always be ruined? If I placed it in formalin and processed it in paraffin what do you suppose it would look like. These antibodies seem hardy and may work on ffpe tissue. These people are doing dna studies on these frozen heart samples and are not concerned about preserving the tissue and cell morphology. Please provide me with some wisdom here. Thank you. Patsy From scoop <@t> mail.nih.gov Thu Jan 8 14:46:07 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Dacie stain Message-ID: Does anyone have a protocol for the Dacie stain for iron? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From gcallis <@t> montana.edu Thu Jan 8 15:23:16 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] frozen heart again In-Reply-To: Message-ID: <3.0.6.32.20040108142316.00bcee30@gemini.msu.montana.edu> Rena Fail and Vinnie Della Speranza had an article on correcting freezing artifact on muscle in Histologic XXVI (1), May 2003. You can go to Sakuraus.com website and read up on how to correct your problem. It was amazing they were able to do this so nicely and simply. At 01:39 PM 1/8/2004 -0700, you wrote: >I have pieces of frozen human heart that was not optomally frozen (meaning >they just put it in a -70 freezer without cryo protectant OCT or anything). >I cut off a piece while it was still frozen and mounted some in OCT and made >frozen sections. As you can imagine the sections are not good. The muscle >is shredded looking with lots of spaces and unrecognizable as muscle. There >are cells which could be myocytes but who knows. I did some IHC on them and >definately have nuclear envelope staining on some cells. I am labeling with >Lap2 and Lamin A&C. I also tried Ki-67 and CD117-cKit, these were both >completely negative (good neg. control I guess). Negatives were negative. >Is it possible to reserrect this frozen tissue or will it always be ruined? >If I placed it in formalin and processed it in paraffin what do you suppose >it would look like. These antibodies seem hardy and may work on ffpe >tissue. These people are doing dna studies on these frozen heart samples >and are not concerned about preserving the tissue and cell morphology. >Please provide me with some wisdom here. Thank you. >Patsy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Jan 8 15:31:43 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] mouse bile duct epithelium In-Reply-To: Message-ID: <3.0.6.32.20040108143143.00bcee30@gemini.msu.montana.edu> How about trying another mouse to mouse kit that does not involve biotinyated secondaries. The key to mouse to mouse blocking in Scytek's kits are the two blocking reagents, one could use their blockers which they will separately from a kit, use your mouse to mouse primary, and plug in a secondary that is directly conjugated to HRP, eliminating biotin altogether. That could be another possibility. Basically you are setting up your own mouse to mouse kit with some superb blocking reagents. DAKO's avidin biotin block, I have heard, is a higher concentration to deal with CSA or tyramide amplification involving biotin, and may work better unless you are already using DAKO's and increase the time of the avidin/biotin blocking to insure endog biotin in liver is zip. Good luck At 01:24 PM 1/8/2004 -0700, you wrote: >here is my story: >i am trying to label ms bile duct epithelium with mouse anti-mouse >cytokeratin 7 and 19 (sounds odd, but that is what the investigator tells me >it is, not even rat anti-mouse) they tried the anti-human ck7&19 and it did >not cross react with the mouse. I have the reagents to biotinylate these >msbiotin is a huge problem even with the most aggressive A/B blocking. Is >there another possible rabbit antibody I could use that would label ms bile >duct epithelial cells? Would factor VIII, cd31, or cd34 stain these cells? >I know that the previous mentioned markers are for endothelial cells but I >am just hoping they might work????? I have not been able to find any other >say rabbit anti-ms ck7 or ck19 out there, has anyone else. How about the >new rabbit monoclonals, is anyone making ck7/ck19? By the way I have frozen >and ffpe bile duct samples, so I can use either. >Please advise. >Patsy Ruegg > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From shive003 <@t> umn.edu Thu Jan 8 16:27:49 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] mouse bile duct epithelium References: Message-ID: <011801c3d636$a5bd98f0$78065486@vdl220FAC> I have used a rat monoclonal against mouse cytokeratin CK8 (clone TROMA-1; from U of Iowa Hybridoma Bank). It stained well, but at the dilution recommended (1:2) I felt there was nonspecific background as well. With more of a work-up, this could probably be improved. In the following article you will find other mouse CKs listed and their sources- Spontaneous mouse mammary tumors: incidence and cytokeratin expression; Research in Veterinary Science; 1997; 63; 85-89. Jan Shivers Scientist U of MN Vet Diag Lab ----- Original Message ----- From: "Patsy Ruegg" To: "Histonet@Pathology. Swmed. Edu" Sent: Thursday, January 08, 2004 2:24 PM Subject: [Histonet] mouse bile duct epithelium > here is my story: > i am trying to label ms bile duct epithelium with mouse anti-mouse > cytokeratin 7 and 19 (sounds odd, but that is what the investigator tells me > it is, not even rat anti-mouse) they tried the anti-human ck7&19 and it did > not cross react with the mouse. I have the reagents to biotinylate these > ms biotin is a huge problem even with the most aggressive A/B blocking. Is > there another possible rabbit antibody I could use that would label ms bile > duct epithelial cells? Would factor VIII, cd31, or cd34 stain these cells? > I know that the previous mentioned markers are for endothelial cells but I > am just hoping they might work????? I have not been able to find any other > say rabbit anti-ms ck7 or ck19 out there, has anyone else. How about the > new rabbit monoclonals, is anyone making ck7/ck19? By the way I have frozen > and ffpe bile duct samples, so I can use either. > Please advise. > Patsy Ruegg > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From livieira <@t> ualg.pt Fri Jan 9 09:04:26 2004 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Some questions about decalcification and staining Message-ID: <002701c3d6c1$df7bca90$2914100a@labhistologia> Helo Everyone I need your help one more time! What is the better procedure to decalcify bone (fish bone), when the objective is the description histologique, with discrimination between bony and cartilaginous tissue? In our laboratory we can include and cut in parafin or in resin, and I will be grateful if you can take me some advise about procedure in parafin or in resin. Tanks in advance Lina Vieira From RizoC <@t> chi.osu.edu Fri Jan 9 10:10:16 2004 From: RizoC <@t> chi.osu.edu (Rizo, Christian) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Hematoxylin Puris Message-ID: <3F8707A1ADC19C4FA84BC95B51CEF560094B9EF3@chi2kms03.columbuschildrens.net> Hello Histonetters, Can anybody help me out with a Heidenhain's Iron Hematoxylin Stain? It's a special stain that directly demonstrates the parasite Entamoeba Histolytica. Can anybody guide me through where to purchase Regaud's Iron Hematoxylin? One of the key reagents is hematoxylin puris? Any help is appreciated. Chris Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From RFORD <@t> HCMHCARES.ORG Fri Jan 9 11:42:41 2004 From: RFORD <@t> HCMHCARES.ORG (Ford, Rhonda) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] 01/01/05 HT certification deadline Message-ID: <684DF8CDE1FB6A428499DA65D753D12224BEFC@jupiter.hcmh.org> My Laboratory Manager would like to know what type of activities in Histology that a non-certified, non-associate degreed employee will be allowed to do after the 01/01/05 deadline. Thanks in advance for replies! From joeamateur <@t> hotmail.com Fri Jan 9 13:26:41 2004 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Ethanol vs. Isopropanol Message-ID: Hello Histonetters, I apologize if this question has been hashed over before, but I couldn't find it in the archives. I've seen ethanol and isopropanol used pretty much interchangeably in dehydration and staining procedures. From my cell culture experience, I've noticed that isopropanol tends to work slightly better to clean trypan blue off of slides, so here's my question: Are there any reasons why one or the other might be better for dehydration? How about staining? Have any of you out there tried both out and found one better than the other for a given application? Warmest regards to all, and best wishes for a prosperous year!! --Jack England _________________________________________________________________ Take advantage of our limited-time introductory offer for dial-up Internet access. http://join.msn.com/?page=dept/dialup From asmith <@t> mail.barry.edu Fri Jan 9 15:00:08 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Hematoxylin Puris Message-ID: <494304423C63E246A5CF87A3AEEB577011EDD2@bumail01.barrynet.barry.edu> Hematoxylin Puris is an archaic equivalent for certified hematoxylin. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rizo, Christian Sent: Friday, January 09, 2004 11:10 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Hematoxylin Puris Hello Histonetters, Can anybody help me out with a Heidenhain's Iron Hematoxylin Stain? It's a special stain that directly demonstrates the parasite Entamoeba Histolytica. Can anybody guide me through where to purchase Regaud's Iron Hematoxylin? One of the key reagents is hematoxylin puris? Any help is appreciated. Chris Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From pam <@t> ategra.com Fri Jan 9 14:47:35 2004 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Happy New Year! Histology Openings Latest Update 01/09/04 Message-ID: Hi Histotech, I wish you a Happy and Prosperous New Year. Just to let you know last month I placed 5 histology professionals. They are having an especially Happy and Prosperous New Year in new positions with higher pay and career advancement. I have openings for Histo Techs and Histology Supervisors in ME, MA, NY, VA, PA, FL, MO, OR, OH, MD and TX These are full time permanent positions. My clients offer the latest in lab equipment, competitive salaries and excellent benefits. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. I don't want to be a bother - I was told that you were a histo tech. If you are no longer working in the field please send me an email and I will delete you from my list of people to contact. If you are interested in a new position, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). Also, if you have friends/peers who do not have an email address, if you could pass my query & name on to them I'd be much obliged. If for any reason you don't want to hear from me in the future (close to retire, etc.) please send me an email and I will delete your name. However, if you are interested in a new position, please call me. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: Ategra Systems Inc Specialists in Permanent & Contract Staffing 7085 University Blvd. Winter Park, FL 32792 VOICE: 407-671-5800 ext 234 TOLLFREE: 800-466-9919 ext 234 EMAIL: pam@ategra.com WEBSITE: If you received this email in error, or if you wish to receive no more emails from us, please send me an email to the above address and you will be deleted from our prospect list. For faster results, please tell us the email address we used to email you, (in your case it is "Histonet@pathology.swmed.edu". ---------------------------------------------------------------- --------------------------------------------------------- From Barry.R.Rittman <@t> uth.tmc.edu Fri Jan 9 15:57:26 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Hematoxylin Puris Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0635904@UTHEVS3.mail.uthouston.edu> Chris Heidenhains iron hematoxylin is not specific for Entamoeba, it can be used to shown anything from nuclei to muscle striations. The structures demonstrated depend on the degree of differentiation. The stain useful because of the intensity of staining due to the iron-hematoxylin that is formed. Regaud's hematoxylin is a modified Heidenhains and usually, because of glycerol in the mixture gives a little more uniform of a stain. Both depend on mordanting in iron alum followed by hematoxylin and then differentiating in iron alum. Not sure if Regaud's is commercially available but it is not that difficult to make. 5% aqueous iron alum as mordanting and differentiating soln. (I prefer to use a weaker iron alum solution for differentiation for better control. When making this solution make sure that the crystals you use are lilac in color as the crystals tend to have a yellow powder if kept too long. I prefer to use iron alum solutions that are fresh although the solution will keep for several weeks. Hx stain is 1 gm hematoxylin, 10 ml. 90% ethanol, 80 ml. Distilled water, 10 ml. glycerin. This need sto be some weaks old or else ripened before use. >From distilled water, mordant in iron alum soln. For 30 mins plus. Rinse in several changes distilled water. Stain in Regaud's soln. for 30-60 mins. Better staining if you carry out the mordanting and staining at 50-60C. Rinse in distilled water Differentiate in iron alum. Wash well in tap water etc. Barry Hello Histonetters, Can anybody help me out with a Heidenhain's Iron Hematoxylin Stain? It's a special stain that directly demonstrates the parasite Entamoeba Histolytica. Can anybody guide me through where to purchase Regaud's Iron Hematoxylin? One of the key reagents is hematoxylin puris? Any help is appreciated. Chris From Rcartun <@t> harthosp.org Fri Jan 9 16:19:47 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] RE: Hematoxylin Puris Message-ID: Immunohistochemistry is available for Entamoeba histolytica. Richard Cartun From SohrabB <@t> wmmcpo.ah.org Fri Jan 9 16:50:53 2004 From: SohrabB <@t> wmmcpo.ah.org (Behnaz Sohrab) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] UNSIBSCRIBE Message-ID: THIS IS THE 2ND REQUEST. I WILL BE OUT TILL JAN 26TH. From pruegg <@t> colobio.com Fri Jan 9 17:48:53 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Antibody question Message-ID: MessageContact Sara directly, do not reply to this message. Patsy -----Original Message----- From: Garza-Williams, Sara [mailto:Garza-Williams.Sara@tchden.org] Sent: Friday, January 09, 2004 1:31 PM To: csh-officers@yahoogroups.com Subject: [csh-officers] Antibody question Do any of you know of a lab that does Gamma/Delta IHC stains? Need information in ASAP! Thanks Sara Sara Williams Anatomic Pathology Supervisor The Children's Hospital Denver, CO 303.861.6177 garza-williams.sara@tchden.org CONFIDENTIALITY NOTICE: The information contained in this message is legally privileged and confidential information intended for the use of the individual or entity named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any release, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the author immediately by replying to this message and delete the original message. Thank you. Yahoo! Groups Sponsor ADVERTISEMENT ---------------------------------------------------------------------------- ---- Yahoo! Groups Links a.. To visit your group on the web, go to: http://groups.yahoo.com/group/csh-officers/ b.. To unsubscribe from this group, send an email to: csh-officers-unsubscribe@yahoogroups.com c.. Your use of Yahoo! Groups is subject to the Yahoo! Terms of Service. From j.p.g.ooms <@t> zonnet.nl Sun Jan 11 04:49:41 2004 From: j.p.g.ooms <@t> zonnet.nl (Hans Ooms) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] brainfix and Bielschowski Message-ID: <001201c3d830$9ec24ef0$a369a63e@st2c8pdli7fy3i> Hello there dear histolovers ! 1. Does anyone know what the best method is for fixation of whole brain and how long does it last generally? Perfusion is not an option I presume. 2. Are there colleagues who have experience with Bielschowski's silver impregnation for brainslides? There could be prenty of modifications, but what gives a good result? Thanks in advance ! J. Ooms HT, former labtech Rijnstate Arnhem, sectionhead histology Pamm Eindhoven The Netherlands From georgecole <@t> ev1.net Sun Jan 11 13:45:28 2004 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Complaints against gremlins Message-ID: <000001c3d87b$77d487e0$084dbad0@hppav> Gremlins, you say? I have quite a few Of those pesky varmints---a regular zoo--- Tipping things over and thumbs on the scale Streaking my silvers, turning them pale. They sneak into mixtures and sneak in fridges Many, so many disastrous midges---- They come though the windows, they come under doors They lurk behind bottles and hide in the drawers They wreak and they wreck, pestiferous crew With sand in the works, with hardning, with goo!!! So histotech Schmedly, sought frantic respite To catch evr'y varmint, culprit and sprite: He set up a system for catching The Things With trap doors and latches, with bait hung on springs With infinite mazes, with net and with noose To catch the offenders and NOT turn 'em loose! He baited the thing with some cinnamon toast (Twas said that's the goody they fed upon most) He set it all up and all he could get Was a note hung on it of gremlin regret: "We need more nice toast, and, please, ninety two Napkins and pretzels and noggins of brew!" Having feasted with gluttony, fickly they bit The hand that had fed them, in manner---to whit: Reading the nature of Schmedly's device, The gremlins reversed it---O Guile!! In a trice Poor Schmedly got glupped with a Whomp and a Zing---- Leaving his right shoe in front of the thing. From siksik03 <@t> comcast.net Sun Jan 11 16:27:25 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Bielschowski In-Reply-To: <001201c3d830$9ec24ef0$a369a63e@st2c8pdli7fy3i> References: <001201c3d830$9ec24ef0$a369a63e@st2c8pdli7fy3i> Message-ID: Hi HistoNetters For Bielschowsky, see: Minbay FZ, Kahveci Z, Cavusoglu I (2001) Rapid Bielschowsky silver impregnation method using microwave heating. Biotech Histochem 76:233-237 good luck! Steven Slap Microwave Consultant At 11:49 AM +0100 1/11/04, Hans Ooms wrote: >Hello there dear histolovers ! > >Are there colleagues who have experience with Bielschowski's silver >impregnation for brainslides? There could be prenty of >modifications, but what gives a good result? > >Thanks in advance ! > >J. Ooms HT, >former labtech Rijnstate Arnhem, >sectionhead histology Pamm Eindhoven > >The Netherlands From lpwenk <@t> covad.net Sun Jan 11 18:43:53 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] HT Exam Requirements Message-ID: <002a01c3d8a5$27c2ff00$8732fea9@hppav> I just realized that, on an email sent to Histonet earlier this week, I listed the wrong number of credit hours required to take the HT exam via the associate degree route. ASCP requires 12 credits of biology and chemistry (combined). This is in addition to the associate degree or 60 credit hours (90 quarter hours) plus 1 year full time on-the-job histology experience. My previous email had stated 20 credits of biology and chemistry. This is the minimum requirement for admission into my HT program. Not to minimum requirement required by ASCP to take their HT exam. I apologize for listing the wrong number of credits. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> covad.net Sun Jan 11 18:56:28 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Hematoxylin Puris References: <3F8707A1ADC19C4FA84BC95B51CEF560094B9EF3@chi2kms03.columbuschildrens.net> Message-ID: <003501c3d8a6$e95a8420$8732fea9@hppav> For entamoeba histolytica - how about the PASH stain? This parasite has a glycogen store in it, if I remember correctly. Also - Gomori trichrome and Masson trichrome demonstrate this parasite. Most labs have these three stains around. No need to make a new staining solution. (And if memory serves me correctly, the Heidenhain hematoxylin needs to be ripened for at least 1 month before use.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Rizo, Christian" To: Sent: Friday, January 09, 2004 11:10 AM Subject: [Histonet] Hematoxylin Puris > Hello Histonetters, > > > > Can anybody help me out with a Heidenhain's Iron Hematoxylin Stain? It's a > special stain that directly demonstrates the parasite Entamoeba Histolytica. > > > > > Can anybody guide me through where to purchase Regaud's Iron Hematoxylin? > One of the key reagents is hematoxylin puris? > > > > Any help is appreciated. > > > > Chris > > > > > > Confidentiality Notice: This e-mail message, from Children's Hospital, > Columbus, Ohio, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. The recipient is responsible to maintain the confidentiality > of this information and to use the information only for authorized purposes > pursuant to Children's Hospital's confidentiality policies. If you are not > the intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in reliance on > the contents of this e-mail is strictly prohibited. If you have received > this communication in error, please notify us immediately by reply e-mail > and destroy all copies of the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From j.p.g.ooms <@t> zonnet.nl Mon Jan 12 05:00:07 2004 From: j.p.g.ooms <@t> zonnet.nl (Hans Ooms) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] eye-processing Message-ID: <003b01c3d8fb$3e28fb90$5b67a63e@st2c8pdli7fy3i> Dear fellow histofreaks ! For we see eye's rarely in daily routine we normally send these specimens to specialised labs. This has to be STOPPED ! So could anyone help me with the next questions please ? What's the best way of fixing a whole eye for routine sectioning and keeping morphology and cutting capabilities of high standards ? Does anyone have any experience with fixative-injection in the enucleated eye ? (no, not in vivo.......thanks Heaven). My experience is that when a tumor, growing from the retina towards the corpus vitreum and both are attached, the latter tends to fall off during staining and take tumorparticals with it. Thanks again. Hans Ooms The Netherlands From jcox90 <@t> yahoo.com Mon Jan 12 09:21:54 2004 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Histology Supervisor opening in Seattle Message-ID: <20040112152154.64365.qmail@web40905.mail.yahoo.com> Hello everyone, We have a Histology Supervisor position open here in Seattle Wa. This is a great lab with wonderful Doctors and excellent working conditions. If interested please call 206-365-1438. Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From ljb <@t> medicine.wisc.edu Mon Jan 12 09:22:33 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Unable to get to the testing info Message-ID: Hello Histonetters, I've been trying for several days now to get into the ASCPs website for HTL and IHC testing information and Applications for the exams. No luck! Is the ASCPs website experiencing some difficulties? Thanks Cindy LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 From tflore <@t> lsuhsc.edu Mon Jan 12 10:38:35 2004 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Next HT (ASCP) test Message-ID: Will someone please let me know when the next HT (ASCP) test is? Regards, Teresa From tflore <@t> lsuhsc.edu Mon Jan 12 10:38:35 2004 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Next HT (ASCP) test Message-ID: Will someone please let me know when the next HT (ASCP) test is? Regards, Teresa From Jackie.O'Connor <@t> abbott.com Mon Jan 12 12:07:46 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Frozen section ice artifact Message-ID: Yo Histonetters, and Happy New Year. I just cut some frozen sections of solid tumors in OCT which were supposed to be snap frozen in liquid nitrogen after being put in plastic cryomolds. I have never seen such horrible morphology in my career. I think this is an example of the absolute worst ice crystal artifact I have ever seen, but since I have never seen anything this bad before, I don't know what I'm seeing, you see? I hope I've intrigued someone enough to email me directly, so I can send you a gif file of this atrocity. I think the tissues were mishandled prior to freezing (someone else froze them) and I'm trying to figure out what happened so I can correct the problem. I'd appreciate anyone who would like to take a look at this artifact - it's awful, honest! Jackie O'Connor From cathy <@t> wasatchhisto.com Mon Jan 12 12:17:19 2004 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] particle counting Message-ID: <004b01c3d938$5237f220$f7693442@selfuggnbwd3wt> Dear Fellow Histonetters, Is there anyone that is familiar with particle counting? I have no experience in this or even aware of a lab that might could help out. The client needs to count particles caught in a "net" that will range in size of 100-900 microns. Any suggestions? Thank you in advance, Cathy ********************************************************** "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com From Barry.R.Rittman <@t> uth.tmc.edu Mon Jan 12 12:26:21 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] particle counting Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FBD3@UTHEVS3.mail.uthouston.edu> Cathy I am familiar with the image analysis and some of the methodology of particle counting but need some more details. What is the format? Can these particles be freed from the net. What information do you need, numbers, particle ranges, shapes etc. Thanks Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton Sent: Monday, January 12, 2004 12:17 PM To: Histonet Subject: [Histonet] particle counting Dear Fellow Histonetters, Is there anyone that is familiar with particle counting? I have no experience in this or even aware of a lab that might could help out. The client needs to count particles caught in a "net" that will range in size of 100-900 microns. Any suggestions? Thank you in advance, Cathy ********************************************************** "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Mon Jan 12 12:44:21 2004 From: jstaruk <@t> masshistology.com (Mass Histology Service) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] particle counting In-Reply-To: <004b01c3d938$5237f220$f7693442@selfuggnbwd3wt> Message-ID: I use the Scion Image software (freeware from the NIH). It'll do most any kind of particle measuring and analyzing you want to do. Jim ____________________ James Staruk, HT(ASCP) Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cathy Mayton Sent: Monday, January 12, 2004 1:17 PM To: Histonet Subject: [Histonet] particle counting Dear Fellow Histonetters, Is there anyone that is familiar with particle counting? I have no experience in this or even aware of a lab that might could help out. The client needs to count particles caught in a "net" that will range in size of 100-900 microns. Any suggestions? Thank you in advance, Cathy ********************************************************** "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ckbyrne <@t> exelixis.com Mon Jan 12 13:01:10 2004 From: ckbyrne <@t> exelixis.com (Carrie Kyle-Byrne) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] S San Francisco job posting Message-ID: <002001c3d93e$714fc9c0$860c1dac@ckbyrnewkst> I'm going to be joining Tim at Lab Vision soon.....so i thought i'd try to help my current boss out a bit......the position hasn't been assigned a req. # yet.....so if you're interested, email your cv/resume to careers@exelixis.com and indicate the position is in the Antibody Tech group/Belinda Cancilla is the hiring manager. HR will get your info to her. this is a great lab to work in and everyone works together very well. if anyone has any questions about the position, you can email me directly at Exelixis 'til the end of next week. Associate Research Scientist I/II: Description of Function and Responsibility: As part of the Antibody Technology team, you will perform immunohistochemical analysis of target proteins on tissue sections and assist the characterization of a variety of antibodies. You will also perform histological techniques such as tissue processing and embedding, sectioning of paraffin embedded tissues, and histological analysis of specimens by standard (hematoxylin and eosin) and special stains. Requirements: This position requires a Bachelors Degree preferably with a biology background. Laboratory experience is required, particularly in histology and immunohistochemistry. Strong organizational skills and the desire to work in a team environment are essential. Carrie Kyle-Byrne, BHS, HT(ASCP) Assoc. Research Scientist II Antibody Core Lab Signal Transduction Research Exelixis, Inc. 170 Harbor Way P.O. Box 511 South San Francisco CA 94083-0511 USA Phone: (1 650) 837-8023 Fax: (1 650) 837-7220 Email: ckbyrne@exelixis.com FedEx Deliveries and Shipping to: 169 Harbor Way South San Francisco CA 94080 USA ________________________________________________________________ This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From pruegg <@t> colobio.com Mon Jan 12 13:43:24 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Frozen section ice artifact In-Reply-To: Message-ID: Jackie, I am having the same kind of trouble with some mishandled frozen heart samples. I was told that Vinnie and Rena Fall wrote an article for Histologic in ?May 03 on how to repair poorly frozen samples but I spent a lot of time trying to see the article on the Sakura web site this weekend without success. Can someone tell me how to fix this heart sample or what was in their article please. I think it has something to do with melting the block and refreezing it properly. Jackie I would like to see your sample to see if it resembles mine. I cannot recognize my tissue as muscle it is all shredded with spaces etc. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com Sent: Monday, January 12, 2004 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section ice artifact Yo Histonetters, and Happy New Year. I just cut some frozen sections of solid tumors in OCT which were supposed to be snap frozen in liquid nitrogen after being put in plastic cryomolds. I have never seen such horrible morphology in my career. I think this is an example of the absolute worst ice crystal artifact I have ever seen, but since I have never seen anything this bad before, I don't know what I'm seeing, you see? I hope I've intrigued someone enough to email me directly, so I can send you a gif file of this atrocity. I think the tissues were mishandled prior to freezing (someone else froze them) and I'm trying to figure out what happened so I can correct the problem. I'd appreciate anyone who would like to take a look at this artifact - it's awful, honest! Jackie O'Connor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Mon Jan 12 14:08:09 2004 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Sakura VIP 5 Message-ID: <4002FEA9.3060108@rci.rutgers.edu> Hey, Histonetters! We here at the Neurotoxicology Labs at Rutgers University are thinking of buying a Sakura VIP 5 tissue processor, benchtop model. I would appreciate any comments, praise, cursing, advice, etc. from those of you who either have one, or have used one before we send in the PO. Also, are there any other models from any other companies that you think we should investigate? Thank you in advance- Kathleen Roberts Principal LabTechnician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 From ander093 <@t> gold.tc.umn.edu Mon Jan 12 14:09:38 2004 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Frozen section ice artifact In-Reply-To: References: Message-ID: <5.2.0.9.0.20040112140736.00a25c60@ander093.email.umn.edu> Hi Patsy and Jackie, I have the article and would be happy to fax a copy to anyone who needs it. If you'd like me to fax a copy to you, please email me with your fax number and I will get it right off to you. LuAnn Anderson HT(ASCP) Neuropathology Lab University of Minnesota At 12:43 PM 1/12/04 -0700, Patsy Ruegg wrote: >Jackie, >I am having the same kind of trouble with some mishandled frozen heart >samples. I was told that Vinnie and Rena Fall wrote an article for >Histologic in ?May 03 on how to repair poorly frozen samples but I spent a >lot of time trying to see the article on the Sakura web site this weekend >without success. Can someone tell me how to fix this heart sample or what >was in their article please. I think it has something to do with melting >the block and refreezing it properly. Jackie I would like to see your >sample to see if it resembles mine. I cannot recognize my tissue as muscle >it is all shredded with spaces etc. >Patsy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >Jackie.O'Connor@abbott.com >Sent: Monday, January 12, 2004 11:08 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Frozen section ice artifact > > >Yo Histonetters, and Happy New Year. > >I just cut some frozen sections of solid tumors in OCT which were supposed >to be snap frozen in liquid nitrogen after being put in plastic cryomolds. >I have never seen such horrible morphology in my career. I think this is >an example of the absolute worst ice crystal artifact I have ever seen, >but since I have never seen anything this bad before, I don't know what >I'm seeing, you see? I hope I've intrigued someone enough to email me >directly, so I can send you a gif file of this atrocity. I think the >tissues were mishandled prior to freezing (someone else froze them) and >I'm trying to figure out what happened so I can correct the problem. I'd >appreciate anyone who would like to take a look at this artifact - it's >awful, honest! > >Jackie O'Connor >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Mon Jan 12 14:16:40 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Frozen section ice artifact Message-ID: Remember, you can post pictures on Histonet.org and then refer to them by name in your email to the regular histonet. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie.O'Connor@abbott.com Sent: Monday, January 12, 2004 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section ice artifact Yo Histonetters, and Happy New Year. I just cut some frozen sections of solid tumors in OCT which were supposed to be snap frozen in liquid nitrogen after being put in plastic cryomolds. I have never seen such horrible morphology in my career. I think this is an example of the absolute worst ice crystal artifact I have ever seen, but since I have never seen anything this bad before, I don't know what I'm seeing, you see? I hope I've intrigued someone enough to email me directly, so I can send you a gif file of this atrocity. I think the tissues were mishandled prior to freezing (someone else froze them) and I'm trying to figure out what happened so I can correct the problem. I'd appreciate anyone who would like to take a look at this artifact - it's awful, honest! Jackie O'Connor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Jan 12 14:52:00 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Frozen section ice artifact Message-ID: Thanks, Charles! I have sent the gif file to Histonet.org as "Freezing artifact Jan12". I forgot about that feature, but I did remember that I'm not supposed to attach files to postings! Thanks to everyone who responded to my plight. The general consensus is slow freezing (not methybutane in liquid nitrogen) in a -70 freezer or cryostat caused this large ice crystal artifact. I love you guys! Jackie O' "Charles Scouten" 01/12/2004 02:16 PM To: , cc: Subject: RE: [Histonet] Frozen section ice artifact Remember, you can post pictures on Histonet.org and then refer to them by name in your email to the regular histonet. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie.O'Connor@abbott.com Sent: Monday, January 12, 2004 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section ice artifact Yo Histonetters, and Happy New Year. I just cut some frozen sections of solid tumors in OCT which were supposed to be snap frozen in liquid nitrogen after being put in plastic cryomolds. I have never seen such horrible morphology in my career. I think this is an example of the absolute worst ice crystal artifact I have ever seen, but since I have never seen anything this bad before, I don't know what I'm seeing, you see? I hope I've intrigued someone enough to email me directly, so I can send you a gif file of this atrocity. I think the tissues were mishandled prior to freezing (someone else froze them) and I'm trying to figure out what happened so I can correct the problem. I'd appreciate anyone who would like to take a look at this artifact - it's awful, honest! Jackie O'Connor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCarpenter764 <@t> aol.com Mon Jan 12 15:02:38 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] calculators and the HT exam Message-ID: <48.26a992f8.2d34656e@aol.com> Does anyone know from experience if we will be able to use a calculator for the HT exam for the mathematics and solution preparation part? thanks for any kind of help From GDawson <@t> Milw.Dynacare.com Mon Jan 12 15:18:58 2004 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] CPT coding for IHC Message-ID: All, I am in need of some clarification. Please let me know which answer you would chose for the following scenario. A pathologist orders a CD3, CD20, CD15 & CD30 on blocks A1, A4, B2 & C2 from case S04-100. So 16 total IHC stains are performed on this case. In coding for this one would you chose: A) 88342 X 16 to cover all IHC's done. B) 88342 X 4 to cover the 4 different IHC's done. C) 88342 X 12 to avoid double billing on the "A" subnumber. I have been told that each one of these was the correct answer depending on who I am asking at the time. My confusion cup runneth over. Help!!, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI From csawrenk <@t> bccancer.bc.ca Mon Jan 12 15:33:54 2004 From: csawrenk <@t> bccancer.bc.ca (Christina Sawrenko) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] Parathyroid Hormone Antibody Message-ID: <6BAF4D075F07D411B30900508B94CBA09C6D2A@SERVER20> Hello, One of our pathologists has asked about antibody to parathyroid hormone. Is anyone using this antibody and if so, which supplier do you use? Does it perform well for you - is it clean/dirty; any other problems? Thanks for your help! Chris Sawrenko Histopathology British Columbia Cancer Agency Vancouver BC, Canada From JCarpenter764 <@t> aol.com Mon Jan 12 15:34:52 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] ht exam and formulas Message-ID: Can someone also tell me wether or not the formulas are provided for solution preparation or not From JWEEMS <@t> sjha.org Mon Jan 12 15:38:05 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] CPT coding for IHC Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44B6D918@exch4.sjha.org> Correct answer is "C". What do I win? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, Glen Sent: Monday, January 12, 2004 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding for IHC All, I am in need of some clarification. Please let me know which answer you would chose for the following scenario. A pathologist orders a CD3, CD20, CD15 & CD30 on blocks A1, A4, B2 & C2 from case S04-100. So 16 total IHC stains are performed on this case. In coding for this one would you chose: A) 88342 X 16 to cover all IHC's done. B) 88342 X 4 to cover the 4 different IHC's done. C) 88342 X 12 to avoid double billing on the "A" subnumber. I have been told that each one of these was the correct answer depending on who I am asking at the time. My confusion cup runneth over. Help!!, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Rcartun <@t> harthosp.org Mon Jan 12 16:10:01 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:24 2005 Subject: [Histonet] CPT coding for IHC Message-ID: I vote for "C", assuming that A1 & A4 are taken from the same specimen (or in this case same part). Richard Cartun >>> "Dawson, Glen" 01/12/04 04:18PM >>> All, I am in need of some clarification. Please let me know which answer you would chose for the following scenario. A pathologist orders a CD3, CD20, CD15 & CD30 on blocks A1, A4, B2 & C2 from case S04-100. So 16 total IHC stains are performed on this case. In coding for this one would you chose: A) 88342 X 16 to cover all IHC's done. B) 88342 X 4 to cover the 4 different IHC's done. C) 88342 X 12 to avoid double billing on the "A" subnumber. I have been told that each one of these was the correct answer depending on who I am asking at the time. My confusion cup runneth over. Help!!, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Jan 12 16:11:03 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Parathyroid Hormone Antibody Message-ID: DakoCytomation has a rat monoclonal anti-PTH antibody that we have used with excellent results. Richard Cartun >>> Christina Sawrenko 01/12/04 04:33PM >>> Hello, One of our pathologists has asked about antibody to parathyroid hormone. Is anyone using this antibody and if so, which supplier do you use? Does it perform well for you - is it clean/dirty; any other problems? Thanks for your help! Chris Sawrenko Histopathology British Columbia Cancer Agency Vancouver BC, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Jan 12 17:00:41 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] CPT coding for IHC Message-ID: I would bill "c". 12 immuno's. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com] Sent: Monday, January 12, 2004 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding for IHC All, I am in need of some clarification. Please let me know which answer you would chose for the following scenario. A pathologist orders a CD3, CD20, CD15 & CD30 on blocks A1, A4, B2 & C2 from case S04-100. So 16 total IHC stains are performed on this case. In coding for this one would you chose: A) 88342 X 16 to cover all IHC's done. B) 88342 X 4 to cover the 4 different IHC's done. C) 88342 X 12 to avoid double billing on the "A" subnumber. I have been told that each one of these was the correct answer depending on who I am asking at the time. My confusion cup runneth over. Help!!, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From michael_lafriniere <@t> memorial.org Mon Jan 12 17:15:54 2004 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] CPT coding for IHC Message-ID: Glen, Were the sites to specimens A, B & C different? Michael LaFriniere PA, HT(ASCP) Pathology & Practice Manager DPS & Memorial Hospital -----Original Message----- From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com] Sent: Monday, January 12, 2004 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding for IHC All, I am in need of some clarification. Please let me know which answer you would chose for the following scenario. A pathologist orders a CD3, CD20, CD15 & CD30 on blocks A1, A4, B2 & C2 from case S04-100. So 16 total IHC stains are performed on this case. In coding for this one would you chose: A) 88342 X 16 to cover all IHC's done. B) 88342 X 4 to cover the 4 different IHC's done. C) 88342 X 12 to avoid double billing on the "A" subnumber. I have been told that each one of these was the correct answer depending on who I am asking at the time. My confusion cup runneth over. Help!!, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> covad.net Mon Jan 12 19:29:16 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] ht exam and formulas References: Message-ID: <004901c3d974$a921bc60$8732fea9@hppav> Chemical formula or mathematical formula? If, on the HT or HTL exam, they ask you how many grams are needed to make a molar solution - they will provide you with the atomic weight of each atom. And they will provide you with the formula of the chemical. But they will NOT provide you with the mathematical formula to solve the equation. An example might be: How many grams are needed to make 200 mL of a 0.5 Molar solution of aluminum hydroxide Al(OH)3? Al = 27, O = 16, H = 1. You would have to know how to figure the molecular weight ((1x27) + (3x16) + (3x1) = 78). You would then know the mathematical formula for molar solutions: g = M x V x MW And then know how to solve it. g = 0.5 x (200/1000) x 78 = 7.8 g (if I figured this correctly) The same is true for all the lab math formulae (percent solutions, dilutions, temperature conversion, gravimetric factors, hydrates, etc.). Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Monday, January 12, 2004 4:34 PM Subject: [Histonet] ht exam and formulas > Can someone also tell me wether or not the formulas are provided for solution > preparation or not > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Mon Jan 12 19:30:34 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] calculators and the HT exam References: <48.26a992f8.2d34656e@aol.com> Message-ID: <005501c3d974$d7a73880$8732fea9@hppav> I believe they supply everything - pads of paper, pencils, calculators. No one is allowed to bring anything into the testing room - no purses, etc. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Monday, January 12, 2004 4:02 PM Subject: [Histonet] calculators and the HT exam > Does anyone know from experience if we will be able to use a calculator for > the HT exam for the mathematics and solution preparation part? thanks for > any kind of help > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Mon Jan 12 19:36:44 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Next HT (ASCP) test References: Message-ID: <006501c3d975$b3fb55a0$8732fea9@hppav> The next deadline is April 1, 2004, for the July-Sept. 2004 exams. This is for any ASCP BOR exam http://www.ascp.org/bor/certification/procedures/deadline.asp Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Flores, Teresa" To: Cc: Sent: Monday, January 12, 2004 11:38 AM Subject: [Histonet] Next HT (ASCP) test > Will someone please let me know when the next HT (ASCP) test is? > Regards, Teresa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Mon Jan 12 19:36:44 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Next HT (ASCP) test References: Message-ID: <006501c3d975$b3fb55a0$8732fea9@hppav> The next deadline is April 1, 2004, for the July-Sept. 2004 exams. This is for any ASCP BOR exam http://www.ascp.org/bor/certification/procedures/deadline.asp Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Flores, Teresa" To: Cc: Sent: Monday, January 12, 2004 11:38 AM Subject: [Histonet] Next HT (ASCP) test > Will someone please let me know when the next HT (ASCP) test is? > Regards, Teresa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Mon Jan 12 19:47:27 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Practical Exams Message-ID: <007c01c3d977$36d0e020$8732fea9@hppav> Hi - I just discovered that the ASCP Board of Registry (BOR) web page has the practical exam lists for both the HT and HTL exams - deadline March 30, 2004 (Spring 2004 exam). http://www.ascp.org/bor/index.asp So if you already signed up for the written exam Jan-June 2004, and haven't received your practical list yet, you can get it on line. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> covad.net Mon Jan 12 19:34:07 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Unable to get to the testing info References: Message-ID: <005e01c3d975$56be02c0$8732fea9@hppav> I am able to get in at work on Friday, and from home on Monday. http://www.ascp.org/bor You need Adobe 6.0 to get some of the pages. There is a way from the website to download updated versions of Adobe. See if that's the problem. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "LaCinda Burchell" To: Sent: Monday, January 12, 2004 10:22 AM Subject: [Histonet] Unable to get to the testing info > Hello Histonetters, > I've been trying for several days now to get into the ASCPs website for > HTL and IHC testing information and Applications for the exams. No > luck! Is the ASCPs website experiencing some difficulties? Thanks > Cindy > > > LaCinda Burchell, BA, AS, HT(ASCP) > University of Wisconsin-Madison, Medical School > Asthma and Allergy Research IHC Lab > 600 Highland Ave. CSC K4/913 > Madison, Wisconsin 53792 > > Phone: 608-262-3518 > FAX: 608-263-3746 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Mon Jan 12 22:30:23 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Sakura VIP 5 References: <4002FEA9.3060108@rci.rutgers.edu> Message-ID: <00a301c3d98d$f8ee78a0$70494542@satx.rr.com> Kathleen, my VIP 5 is a year old and I had to replace the rotary valve twice (under the warranty of course) and it still sounds pretty rough when pumping in solutions. Also, you have to make sure that you secure the lid very well or when the retort pressurizes, the lid pops open and processing stops. Other than that, it's okay, but I still like the old VIPs better. Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas ----- Original Message ----- From: "Kathleen Roberts" To: Sent: Monday, January 12, 2004 12:08 PM Subject: [Histonet] Sakura VIP 5 > Hey, Histonetters! > > We here at the Neurotoxicology Labs at Rutgers University are thinking > of buying a Sakura VIP 5 tissue processor, benchtop model. I would > appreciate any comments, praise, cursing, advice, etc. from those of you > who either have one, or have used one before we send in the PO. > > Also, are there any other models from any other companies that you think > we should investigate? > > Thank you in advance- > Kathleen Roberts > Principal LabTechnician > Neurotoxicology Laboratories > Dept of Pharmacology and Toxicology > Ernest Mario School of Pharmacy > Rutgers University > 41 B Gordon Rd > Piscataway, NJ 08854 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DMBCMP <@t> aol.com Mon Jan 12 20:50:29 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Sakura VIP Message-ID: Kathleen: We had the same experience as Joe did with the lid poping open on the VIP. After the latch was repaired we have not had any problems with it. Design flaw I think. It has been pretty dependable otherwise. Dannie Blake, HT (ASCP) Histology Lead Tech Fresno Community Hospital Fresno, Ca From DDDeltour <@t> sig.med.navy.mil Mon Jan 12 23:55:26 2004 From: DDDeltour <@t> sig.med.navy.mil (Deltour, Douglas D.(HM2)) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Sakura VIP 5 Message-ID: I have used the VIP 5 floor model, Ventana, and now the Leica TP 1050. I really like the VIP 5 compared to the rest. It is easy to use and operate. The one we used was also dependable. It also could call you at home if there was an error in processing. But who needs a computer talking to them at 2 in the morning?? HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy Supervisor Histology Histology Technician HT FROM US: 01139095564862 DSN: 624-4862 FAX FROM US: 01139095564680 DSN: 624-4680 DISCLAIMER: Confidentiality Notice - This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. **** Informazione Confidenziale - Questa e-Mail, incluso eventuali allegati, contiene informazioni confidenziali intese unicamente alla persona/e a cui e' indirizzata. Se avete ricevuto per errore questo messaggio, cortesemente contattare immediatamente il mittente e cancellare la e-Mail. Grazie****. -----Original Message----- From: Kathleen Roberts [mailto:kgrobert@rci.rutgers.edu] Sent: Monday, January 12, 2004 9:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura VIP 5 Hey, Histonetters! We here at the Neurotoxicology Labs at Rutgers University are thinking of buying a Sakura VIP 5 tissue processor, benchtop model. I would appreciate any comments, praise, cursing, advice, etc. from those of you who either have one, or have used one before we send in the PO. Also, are there any other models from any other companies that you think we should investigate? Thank you in advance- Kathleen Roberts Principal LabTechnician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peter.smale <@t> nhls.ac.za Tue Jan 13 03:04:49 2004 From: peter.smale <@t> nhls.ac.za (Peter Smale) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] unsubcribe Message-ID: <002801c3d9b4$4f8765c0$af06a8c0@PeterSmale> To whom it may concern, Unsubcribe ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- From jqb7 <@t> cdc.gov Tue Jan 13 05:04:58 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Sakura VIP 5 Message-ID: We have a VIP-5 and most recently the Excelsior from Thermo-Electron. The VIP-5 has been great and reliable, just like you'd expect with a VIP. We have only had the Excelsior for a very short time, but so far so good. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Roberts Sent: Monday, January 12, 2004 3:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura VIP 5 Hey, Histonetters! We here at the Neurotoxicology Labs at Rutgers University are thinking of buying a Sakura VIP 5 tissue processor, benchtop model. I would appreciate any comments, praise, cursing, advice, etc. from those of you who either have one, or have used one before we send in the PO. Also, are there any other models from any other companies that you think we should investigate? Thank you in advance- Kathleen Roberts Principal LabTechnician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paul.keogh <@t> dental.tcd.ie Tue Jan 13 06:32:33 2004 From: paul.keogh <@t> dental.tcd.ie (Paul Keogh) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Osteoclast stain Message-ID: <2C3FCB82BFFCD3118E6300A0C9E048223134FF@stack.dental.tcd.ie> I have sections of MMA embedded rat femur . I want to stain for osteoclasts. Can anyone recommend a tried and tested protocol please.? Paul ********************************************************************** This email is confidential and intended solely for the use of the individual to whom it is addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Dublin Dental School and Hospital. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error, please contact the sender. ********************************************************************** From tflore <@t> lsuhsc.edu Tue Jan 13 07:28:29 2004 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:25 2005 Subject: FW: [Histonet] Practical Exams Message-ID: IF YOU HAVE NOT REGISTERED TO TAKE THE HT (ASCP) REGISTRY, PLEASE DO IT NOW! IF YOU PASS, GREAT! IF NOT, YOU WILL BE "LOCKED IN" TO BE ABLE TO TAKE THE HT (ASCP) Registry FIVE, THAT'S RIGHT, UP TO FIVE MORE TIMES! Teresa Also, histology registry study group will meet this Saturday, January 17, 2004 from 8am until Noon, at LSUHSC 1901 Perdido, New Orleans, LA, 3rd Floor Seminar Room 8, I will make coffee and bring some donuts. Teresa -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Monday, January 12, 2004 7:47 PM To: Histonet Subject: [Histonet] Practical Exams Hi - I just discovered that the ASCP Board of Registry (BOR) web page has the practical exam lists for both the HT and HTL exams - deadline March 30, 2004 (Spring 2004 exam). http://www.ascp.org/bor/index.asp So if you already signed up for the written exam Jan-June 2004, and haven't received your practical list yet, you can get it on line. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> Milw.Dynacare.com Tue Jan 13 07:51:45 2004 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] CPT coding for IHC Message-ID: All, Just an FYI. I said option "C" was correct. Those who were billing the IHC's said "A" was correct. Those ordering the tests said "B" was correct. So anyone choosing "C" wins an all expense paid vacation to Death Valley courtesy of anyone who said "B" since they save so much money on their IHC's. Thank-You All, Glen D. From pzeitlow <@t> bbpllab.com Tue Jan 13 08:34:17 2004 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] CPT coding for IHC Message-ID: <813FB33DA405334F947F8BFC6EBD0B2A0BE8E0@bbplsrv1.bbpl> Assuming the different blocks are not a "single specimen" C would be the correct answer. If the blocks represent a "single specimen" B is the correct answer. Pat -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Monday, January 12, 2004 3:38 PM To: 'Dawson, Glen'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CPT coding for IHC Correct answer is "C". What do I win? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, Glen Sent: Monday, January 12, 2004 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding for IHC All, I am in need of some clarification. Please let me know which answer you would chose for the following scenario. A pathologist orders a CD3, CD20, CD15 & CD30 on blocks A1, A4, B2 & C2 from case S04-100. So 16 total IHC stains are performed on this case. In coding for this one would you chose: A) 88342 X 16 to cover all IHC's done. B) 88342 X 4 to cover the 4 different IHC's done. C) 88342 X 12 to avoid double billing on the "A" subnumber. I have been told that each one of these was the correct answer depending on who I am asking at the time. My confusion cup runneth over. Help!!, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From fmonson <@t> wcupa.edu Tue Jan 13 10:07:02 2004 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] particle counting - Image Analysis Message-ID: Try this for a start: http://www.pmeasuring.com/education/college For beginning image analysis: Russ: http://www.drjohnruss.com/images/handout.pdf ImageJ(Free program): http://rsb.info.nih.gov/ij/ Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Cathy Mayton [mailto:cathy@wasatchhisto.com] Sent: Monday, January 12, 2004 1:17 PM To: Histonet Subject: [Histonet] particle counting Dear Fellow Histonetters, Is there anyone that is familiar with particle counting? I have no experience in this or even aware of a lab that might could help out. The client needs to count particles caught in a "net" that will range in size of 100-900 microns. Any suggestions? Thank you in advance, Cathy ********************************************************** "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From info <@t> instrumedics.com Tue Jan 13 10:41:05 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] ice crystal artifact References: Message-ID: <012a01c3d9f4$0a1f0ac0$6501a8c0@INSTRUMEDICS22> Jackie, Please send the pictures to us. We developed the Gentle Jane Snap Freezer to minimize ice-crystal artifact. The method provides not only for low temperature(LN2 @ -196C) but for thermal exchange. A chrome-plated brass heat extractor is chilled in liquid nitrogen and then placed on the Gentle Jane device. It falls at a controlled rate. For the best freezing it contacts the tissue first, which is located on the top of a mound of the viscous CryoGel embedding medium . The tissue and the embedding medium are frozen simultaneously. See the details of the method on our web site www.instrumedics.com Please contact us with any questions Bernice schiller@instrumedics.com ----- Original Message ----- From: To: Sent: Monday, January 12, 2004 1:07 PM Subject: [Histonet] Frozen section ice artifact > Yo Histonetters, and Happy New Year. > > I just cut some frozen sections of solid tumors in OCT which were supposed > to be snap frozen in liquid nitrogen after being put in plastic cryomolds. > I have never seen such horrible morphology in my career. I think this is > an example of the absolute worst ice crystal artifact I have ever seen, > but since I have never seen anything this bad before, I don't know what > I'm seeing, you see? I hope I've intrigued someone enough to email me > directly, so I can send you a gif file of this atrocity. I think the > tissues were mishandled prior to freezing (someone else froze them) and > I'm trying to figure out what happened so I can correct the problem. I'd > appreciate anyone who would like to take a look at this artifact - it's > awful, honest! > > Jackie O'Connor > ----- Original Message ----- From: To: Sent: Tuesday, January 13, 2004 6:05 AM Subject: Histonet Digest, Vol 2, Issue 12 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > ---------------------------------------------------------------------------- ---- > Today's Topics: > > 1. Frozen section ice artifact (Jackie.O'Connor@abbott.com) > 2. particle counting (Cathy Mayton) > 3. RE: particle counting (Barry R Rittman) > 4. RE: particle counting (Mass Histology Service) > 5. S San Francisco job posting (Carrie Kyle-Byrne) > 6. RE: Frozen section ice artifact (Patsy Ruegg) > 7. Sakura VIP 5 (Kathleen Roberts) > 8. RE: Frozen section ice artifact (LuAnn Anderson) > 9. RE: Frozen section ice artifact (Charles Scouten) > 10. RE: Frozen section ice artifact (Jackie.O'Connor@abbott.com) > 11. calculators and the HT exam (JCarpenter764@aol.com) > 12. CPT coding for IHC (Dawson, Glen) > 13. Parathyroid Hormone Antibody (Christina Sawrenko) > 14. ht exam and formulas (JCarpenter764@aol.com) > 15. RE: CPT coding for IHC (Weems, Joyce) > 16. Re: CPT coding for IHC (Richard Cartun) > 17. Re: Parathyroid Hormone Antibody (Richard Cartun) > 18. RE: CPT coding for IHC (Horn, Hazel V) > 19. RE: CPT coding for IHC (LaFriniere, Mike) > 20. Re: ht exam and formulas (Lee & Peggy Wenk) > 21. Re: calculators and the HT exam (Lee & Peggy Wenk) > 22. Re: Next HT (ASCP) test (Lee & Peggy Wenk) > 23. Re: Next HT (ASCP) test (Lee & Peggy Wenk) > 24. Practical Exams (Lee & Peggy Wenk) > 25. Re: Unable to get to the testing info (Lee & Peggy Wenk) > 26. Re: Sakura VIP 5 (Joe Nocito) > 27. Sakura VIP (DMBCMP@aol.com) > 28. RE: Sakura VIP 5 (Deltour, Douglas D.(HM2)) > 29. unsubcribe (Peter Smale) > 30. RE: Sakura VIP 5 (Bartlett, Jeanine) > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Tue Jan 13 11:21:17 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Society for Applied Immunohistochemistry- 2nd Survey Message-ID: -----Original Message----- From: richarde@greenhosp.org [mailto:richarde@greenhosp.org] Sent: Tuesday, January 13, 2004 6:22 AM To: pruegg@colobio.com Subject: Society for Applied Immunohistochemistry- 2nd Survey >From the Society of Applied Immunohistochemistry: To all members and fellow IHC users: The Second Immunohistochemistry Technical Data Survey is now on-line at www.appliedimmuno.org Please go to the Survey page to view and submit the kappa- lambda survey. Instructions and more details are available on that page. We will publish the results of this survey on the site in late February or early March on the Survey Results page. We encourage your laboratory's participation in this endeavor and anticipate that the aggregate data will aid in the improvement and standardization of IHC practices. SFAI membership will not be required for the surveys themselves and results for the first few, as they will be open for all visitors to the site. Please remember to submit your data only once for each survey. Questions about the survey may be submitted to Dr. Neal Goldstein, Dr. Richard W. Cartun or Dr. Richard N. Eisen (e-mail addresses and phone numbers are available on the web site's Board member directory page). Thank you for your support for the Surveys and SFAI. Please provide any feedback about the surveys via e-mail, fax or phone. ---------------------------------------------------------------------------- ---- Click here to unsubscribe From pruegg <@t> colobio.com Tue Jan 13 11:25:15 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Osteoclast stain In-Reply-To: <2C3FCB82BFFCD3118E6300A0C9E048223134FF@stack.dental.tcd.ie> Message-ID: Paul, If you have not fixed with formalin you could do Acid Phosphatase (TRAP) for osteoclasts, this is the enzyme histochemical method. I can provide you the method if you like. I used a kit from Sigma, I hope someone is still selling it. There is also an antibody but I do not have experience with it. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Paul Keogh Sent: Tuesday, January 13, 2004 5:33 AM To: 'Histonet (E-mail) Subject: [Histonet] Osteoclast stain I have sections of MMA embedded rat femur . I want to stain for osteoclasts. Can anyone recommend a tried and tested protocol please.? Paul ********************************************************************** This email is confidential and intended solely for the use of the individual to whom it is addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Dublin Dental School and Hospital. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error, please contact the sender. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Jan 13 11:31:59 2004 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] CLIA regulations for person's other than pathologists doing gross descriptions Message-ID: <584AE8968B1EE14998210A0D6B97D854055AEE51@mmcmail2.mhsil.com> I know that CLIA has certain regulations but can't put my finger on it right now. Does anyone know the requirements for people to do gross descriptions of small surgicals handy? Questions have come up, such as: 1. Do they need a BS degree? 2. Do they have to have a histology certification? CAP requires us to: 1. List the types of specimens that the non-pathologist is able to do. 2. State whether the nature of the pathologist supervision is direct or indirect. 3. Have a system to evaluate gross descriptions of non-pathologists. thanks for your help. James R. Vickroy BS, HT (ASCP) Technical Supervisor, Surgical Pathology 788-4046 vickroy.jim@mhsil.com This E-Mail and any attachments are intended solely for the addressee(s) and may be legally privileged and /or confidential. If you believe you received this E-Mail in error, please forward it to mhsil@mhsil.com to notify us of the error, then delete it; do not divulge, copy, forward or use the contents in any way. Please be aware that any unauthorized use or disclosure of the contents of this E-Mail may be unlawful. Memorial Health System 701 N. First Street Springfield, IL 62781-0001 217-757-7753 http://www.mhsil.com From ABurnet <@t> chw.edu Tue Jan 13 11:56:44 2004 From: ABurnet <@t> chw.edu (Burnette, Andrew - MHA) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Pathology Billing Message-ID: <9342C1401745A147A1DD93AAEB29C24B01AFCBF4@aznv-msg-002.chw.edu> Hi all - I seem to recall that a hospital may chose the option of globally billing for path services and paying the pathologists from proceeds rather than billing just part A and leaving the pathologists to bill part B. My question is, may a hospital choose to separate professional and technical component billing for hospital inpatient and outpatients while globally billing outreach patients? I have a faint recollection that under one CLIA number an institution may not carve out certain patients in this way, but I can't find an official reference. Thanks in advance! Andy Burnette, St. Josephs Hospital, Phoenix AZ (aburnet@chw.edu) From jryan <@t> sleh.com Tue Jan 13 14:28:10 2004 From: jryan <@t> sleh.com (John Ryan) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Histology position available Message-ID: St. Luke's Hospital in Houston has an open position for a qualified histology technician. The histology laboratory was recently totally renovated and moved into a new area with plenty of space and ventilation for a change. I have an opening from midnight to 8:30am and a second position from 8:30am to 5:00pm. We are a large volume laboratory with the bulk of our routine H & E stains being completed during the night and ready in the early am; therefore the night position will be mainly embedding and microtomy while the day position will include all phases of histology on a rotational basis. Salary and benefits are competitive in the Houston area and the staff that you will be working with are exceptional. Please contact John Ryan, HT(ASCP)HTL via email at jryan@sleh.com or phone 832-355-2643 or fax 832-355-4232. John P Ryan, HT(ASCP)HTL Assistant Administrative Director Pathology St. Luke's Episcopal Hospital 832-355-2643 "CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From eileen_dusek <@t> yahoo.com Tue Jan 13 14:45:19 2004 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Work load Message-ID: <20040113204519.61553.qmail@web11907.mail.yahoo.com> Hello everyone, A Pathologists was told by the "bean crunchers" they were overstaffed. What is your opinion? The lab does approximately 22,000 cases, Immunos on the Benchmark and a few Special Stains. The gross room is covered 95% of the time by Support Techs. This work load is performed by 2 FTE's and 1 Bench Supervisor. As we know the supervisor cannot bench all the time. Thanks Eileen Dusek --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From Raoul.Regnault <@t> interiorhealth.ca Tue Jan 13 15:22:39 2004 From: Raoul.Regnault <@t> interiorhealth.ca (Regnault, Raoul) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: <1DA1FB459D6ED44EB804DB0C8BE230951B05DB@dc1serv2> The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC From JWEEMS <@t> sjha.org Tue Jan 13 15:27:30 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44B6D927@exch4.sjha.org> My experience has been with Lanier systems. Perhaps you could look on line? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Regnault, Raoul Sent: Tuesday, January 13, 2004 4:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Dale.Waldner <@t> nw.amedd.army.mil Tue Jan 13 15:29:20 2004 From: Dale.Waldner <@t> nw.amedd.army.mil (Waldner, Dale L MAJ MAMC) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Work load Message-ID: <7F001DE4B412D511A64200A0C9EA53A007B6ECC6@dasmthguh002.amedd.army.mil> I am also very interested in any guidelines for evaluating productivity for histotechs. I have searched extensively for any standards but have come up empty-handed. I did find a reference to prior numbers being published based on the College of American Pathologists Laboratory Management Index Program (LMIP), but it is rather old and I believe, excluded automation in the laboratory. The LMIP seems like a great tool but would take some participation time prior to coming up with any meaningful data. Are there any standards available to give managers a general ballpark idea, for starters. The number of personnel Ms. Dusek has for her lab seems maybe even less than adequate to me, but who knows? Thank you. DW -----Original Message----- From: eileen dusek [mailto:eileen_dusek@yahoo.com] Sent: Tuesday, January 13, 2004 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Work load Hello everyone, A Pathologists was told by the "bean crunchers" they were overstaffed. What is your opinion? The lab does approximately 22,000 cases, Immunos on the Benchmark and a few Special Stains. The gross room is covered 95% of the time by Support Techs. This work load is performed by 2 FTE's and 1 Bench Supervisor. As we know the supervisor cannot bench all the time. Thanks Eileen Dusek --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kennedya <@t> email.cs.nsw.gov.au Tue Jan 13 15:53:17 2004 From: kennedya <@t> email.cs.nsw.gov.au (Andrew Kennedy) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Sakura VIP 5 Message-ID: <001d01c3da1f$a6e6f0e0$94e04c98@LAB0216.cs.nsw.gov.au> Kathleen, We have been using the Shandon Excelsior for over a year now and have found it to be a very good machine. The reagent management system is great. The only problems we have had have been user errors because our techs are not as familiar with it as with our other machines - an old VIP workhorse and a Leica TP1050 which I would happily throw into Sydney Harbour!!! Andrew Kennedy Senior Science Officer in charge - Histopathology. Department of Anatomical Pathology Concord Repatriation General Hospital Hospital Road Concord, NSW, Australia 2139 Phone: +612 9767 6115 Fax: +612 9767 8427 "Noli illegitimi carborundum" ------------------------------------------------------------------------------------------------------------ This message is intended for the addressee(s) named and may contain confidential information. If you are not an intended recipient, please delete this email and notify the sender. Views expressed in this message are those of the individual sender and are not necessarily the views of Central Sydney Area Health Service. ------------------------------------------------------------------------------------------------------------ From JCarpenter764 <@t> aol.com Tue Jan 13 16:23:04 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: <48.26b41a0d.2d35c9c8@aol.com> OK IM CONFUSED.......can someone please explain to me how this works....i can't seem to calculate the correct answers if you have time maybe you can talk me through this one problem on finding the molar solution and the normal solution...HERE IT GOES.... this is the question.... How much of each of the following chemicals is needed to prepare 1000 mL of both a 1M and a 1N solution?... Atomic Weights: Na=22.99, Cl=35.45 1. NaCl Thanks a bunch From asmith <@t> mail.barry.edu Tue Jan 13 17:00:46 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: <494304423C63E246A5CF87A3AEEB577011EDD7@bumail01.barrynet.barry.edu> Since the formula is NaCl, 1 X 22.99 + 1 X 35.45 = 58.44 daltons is the molecular weight. 58.44 grams of NaCl dissolved in enough water to make 1000 ml (i.e., 1 L) will give you a 1 molar solution. [Avogadro's number of molecules in each liter.] This solution will also be 1 normal in Na+ and 1 normal in Cl-. [Normality is the molarity of a particular atom or ion.] Since NaCl dissociates in solution, this solution will be 2 osmolar. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JCarpenter764@aol.com Sent: Tuesday, January 13, 2004 5:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] normal and molar solutions OK IM CONFUSED.......can someone please explain to me how this works....i can't seem to calculate the correct answers if you have time maybe you can talk me through this one problem on finding the molar solution and the normal solution...HERE IT GOES.... this is the question.... How much of each of the following chemicals is needed to prepare 1000 mL of both a 1M and a 1N solution?... Atomic Weights: Na=22.99, Cl=35.45 1. NaCl Thanks a bunch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From JCarpenter764 <@t> aol.com Tue Jan 13 17:09:55 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Off the Subject .....thank you Message-ID: <109.2b87a90d.2d35d4c3@aol.com> I just wanted to thank everyone for there help. I think this mailing list is the best thing ever....I have worked very hard at preparing myself for this exam and everytime i get stuck and ask a question...someone is always there to lend a helping hand and great advice.. Thanks again i really appreciate it Jennell From nick.kirk3 <@t> btopenworld.com Tue Jan 13 17:15:48 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] RE: normal and molar solutions In-Reply-To: <48.26b41a0d.2d35c9c8@aol.com> Message-ID: As far as my addled brain can remember I think it goes like this. Molar Solutions. The molecular weight of a sodium chloride molecule (NaCl) is 58.44, so one gram-molecular mass (=1 mole) is 58.44 g. If you dissolve 58.44 g of NaCl in a final volume of 1 litre of water, you have made a 1M NaCl solution, i.e. a 1 molar solution. Normal Solutions. A Normal solution contains in each cubic centimetre of water, as many milligrams of the molecule in question as the number representing its atomic weight; thus, a normal solution of sodium chloride would contain 58.44 mg of Sodium chloride in each cubic centimetre of water. Hope that explains it. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JCarpenter764@aol.com Sent: 13 January 2004 22:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] normal and molar solutions OK IM CONFUSED.......can someone please explain to me how this works....i can't seem to calculate the correct answers if you have time maybe you can talk me through this one problem on finding the molar solution and the normal solution...HERE IT GOES.... this is the question.... How much of each of the following chemicals is needed to prepare 1000 mL of both a 1M and a 1N solution?... Atomic Weights: Na=22.99, Cl=35.45 1. NaCl Thanks a bunch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Tue Jan 13 17:40:26 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20356DB@usca0082k08.labvision.apogent.com> What do you call a tooth in a glass of water? A one molar solution. From: http://www.coolscience.org/CoolScience/CoolJokes/ChemJokes.htm Tim Morken -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Tuesday, January 13, 2004 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] normal and molar solutions OK IM CONFUSED.......can someone please explain to me how this works....i can't seem to calculate the correct answers if you have time maybe you can talk me through this one problem on finding the molar solution and the normal solution...HERE IT GOES.... this is the question.... How much of each of the following chemicals is needed to prepare 1000 mL of both a 1M and a 1N solution?... Atomic Weights: Na=22.99, Cl=35.45 1. NaCl Thanks a bunch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tigersnake <@t> ecybermind.net Tue Jan 13 20:54:17 2004 From: tigersnake <@t> ecybermind.net (Paul Howard Lockwood) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Work load Message-ID: <200401132350.i0DNoql14118@ginsberg.ecybermind.net> Dear Eileen, The problem of work load versus staffing comes from the inappropriate application of of a branch of business math called Operations Research. Bean counters have this awful tendancy to work up a model based upon what they consider important functions. This leaves out "unimportant" functions that lab techs must perfrom, including paperwork, washing labware, taking finished work to the pathologists, storing specimens, and so forth. All of these functions do take up time, but somehow the time taken is left out of the model the accountants and business managers and adminstrators come up with. They seem to believe that a lab tech can somehow move themselves into a dimension where time does not occur. Obviously, those of us who actually perform know different, but can;t get this across to the ones who have come up the model. This adherence to the model comes from a belief that the model is the same as real life. None of the people who use Operations Research have ever bothered to read Rutherford Aris's work "Mathematical Models and Modeling Techniques." In this volume, Dr. Aris spends the entire first chapter pointing out that models are not real life, but only models. After the model is devised, it has to be tested. After the test, the data from the test will usually be at variance with the predictions given from the model. Based on those variances, the model has to be reworked to fit the test data, not the other way around. Business people are not scientists, even if they use something that seems scientific. This is what makes them frustrating, and occassionally dangerous in the work place. (The danger being not just loss of jobs of the lab techs, in your case, but turn around time for diagnosis, and hence treatment of patients.) My advice is to do a study of what the lab techs do, and how long it takes them to do each task, and leave nothing out. Present this information to the accountants and administrators, and let them know, firmly but gently, that to reduce your staff would cause severe problems in getting the work done. An example you might use is one from the post office. Postal route carriers had their staffs reduced to the point where one carrier was doing what had been formerly two routes. It was deemed possible to get all this work done in an eight hour day. What was left out was the amount of sorting, and paperwork route carriers have to do. This increased their hours per day from eight to ten, eleven, even twelve hours a day. Where the post office saved money by reducing it's route carrier staff, it lost more money by paying out time and half for the remaining postal carriers. Another example is from where I work, where they have used "lean manufacturing" principles to reduced the staff to the point where there isn't enough people to do the work. And if one person is sick, or takes a vacation, then deadlines are missed. Or, again, money saved from the staff reduction is lost from the time and half paid out for the amount of overtime worked to make up the slack. And, recently, the staff has voluntarily reduced itself by just quitting. Stressed out employees, even in a bad job market, would rather take the risk of being without a job for a while than stay where they will only be on the receiving end of more stress, and abuse. I hope this helps in some way. Good luck to you. Sincerely, Paul Lockwood 1/13/04 12:45:19 PM, eileen dusek wrote: >Hello everyone, >A Pathologists was told by the "bean crunchers" they were overstaffed. What is your opinion? >The lab does approximately 22,000 cases, Immunos on the Benchmark and a few Special Stains. The gross room is covered 95% of the time by Support Techs. This work load is performed by 2 FTE's and 1 Bench Supervisor. As we know the supervisor cannot bench all the time. > >Thanks >Eileen Dusek > > >--------------------------------- >Do you Yahoo!? >Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tpmorken <@t> labvision.com Tue Jan 13 18:08:40 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Work load Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20356DC@usca0082k08.labvision.apogent.com> Paul, thanks for those insights. It reminds me of the old CAP workload counting surveys we used to do. It never counted up all the time in the day so the bean counters complained. It kept getting bigger and counted more and more things - down to the ridiculous, like how many times you put on and take off gloves during the day! Finally we were spending a significant amount of time each day simply tallying up all the workload units (yes, they had a count for that!). It finally became clear it was an unworkable system and was dropped. The intangibles are what make the difference. Like you say, delivering something can take on a big importance. If you have to go to the OR, and it is 5 min away in one lab, but 10 min away in another, how do you account for that? Is it work? What about your work you leave behind that is delayed? Such trips can easily add up to an hour or more each day. In Eileens case it seems that they are risking cutting back so far that the work will not get done on days someone calls in sick. That can't be accounted for in workload figures, no matter how you do it. Tim Morken Lab Vision / Neomarkers www.labvision.com -----Original Message----- From: Paul Howard Lockwood [mailto:tigersnake@ecybermind.net] Sent: Tuesday, January 13, 2004 6:54 PM To: eileen dusek Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Work load Dear Eileen, The problem of work load versus staffing comes from the inappropriate application of of a branch of business math called Operations Research. Bean counters have this awful tendancy to work up a model based upon what they consider important functions. This leaves out "unimportant" functions that lab techs must perfrom, including paperwork, washing labware, taking finished work to the pathologists, storing specimens, and so forth. All of these functions do take up time, but somehow the time taken is left out of the model the accountants and business managers and adminstrators come up with. They seem to believe that a lab tech can somehow move themselves into a dimension where time does not occur. Obviously, those of us who actually perform know different, but can;t get this across to the ones who have come up the model. This adherence to the model comes from a belief that the model is the same as real life. None of the people who use Operations Research have ever bothered to read Rutherford Aris's work "Mathematical Models and Modeling Techniques." In this volume, Dr. Aris spends the entire first chapter pointing out that models are not real life, but only models. After the model is devised, it has to be tested. After the test, the data from the test will usually be at variance with the predictions given from the model. Based on those variances, the model has to be reworked to fit the test data, not the other way around. Business people are not scientists, even if they use something that seems scientific. This is what makes them frustrating, and occassionally dangerous in the work place. (The danger being not just loss of jobs of the lab techs, in your case, but turn around time for diagnosis, and hence treatment of patients.) My advice is to do a study of what the lab techs do, and how long it takes them to do each task, and leave nothing out. Present this information to the accountants and administrators, and let them know, firmly but gently, that to reduce your staff would cause severe problems in getting the work done. An example you might use is one from the post office. Postal route carriers had their staffs reduced to the point where one carrier was doing what had been formerly two routes. It was deemed possible to get all this work done in an eight hour day. What was left out was the amount of sorting, and paperwork route carriers have to do. This increased their hours per day from eight to ten, eleven, even twelve hours a day. Where the post office saved money by reducing it's route carrier staff, it lost more money by paying out time and half for the remaining postal carriers. Another example is from where I work, where they have used "lean manufacturing" principles to reduced the staff to the point where there isn't enough people to do the work. And if one person is sick, or takes a vacation, then deadlines are missed. Or, again, money saved from the staff reduction is lost from the time and half paid out for the amount of overtime worked to make up the slack. And, recently, the staff has voluntarily reduced itself by just quitting. Stressed out employees, even in a bad job market, would rather take the risk of being without a job for a while than stay where they will only be on the receiving end of more stress, and abuse. I hope this helps in some way. Good luck to you. Sincerely, Paul Lockwood 1/13/04 12:45:19 PM, eileen dusek wrote: >Hello everyone, >A Pathologists was told by the "bean crunchers" they were overstaffed. >What is your opinion? The lab does approximately 22,000 cases, Immunos >on the Benchmark and a few Special Stains. The gross room is covered 95% of the time by Support Techs. This work load is performed by 2 FTE's and 1 Bench Supervisor. As we know the supervisor cannot bench all the time. > >Thanks >Eileen Dusek > > >--------------------------------- >Do you Yahoo!? >Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jan 13 21:43:37 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] CLIA regulations for person's other than pathologists doing gross descriptions References: <584AE8968B1EE14998210A0D6B97D854055AEE51@mmcmail2.mhsil.com> Message-ID: <003301c3da50$9b70b6c0$70494542@satx.rr.com> Jim, one must have been trained before 1995 if they do not have any college credit. After 1995, you must have at least 60 semester hours, with courses in chemistry, biology, anatomy. You do not have to be certified, but your procedures must include the types of specimens one may gross under the direct supervision of a pathologist as well as documentation of at least annual performance. Hope this helps. Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas ----- Original Message ----- From: "Vickroy, Jim" To: Sent: Tuesday, January 13, 2004 9:31 AM Subject: [Histonet] CLIA regulations for person's other than pathologists doing gross descriptions > I know that CLIA has certain regulations but can't put my finger on it right > now. > > Does anyone know the requirements for people to do gross descriptions of > small surgicals handy? > > Questions have come up, such as: > 1. Do they need a BS degree? > 2. Do they have to have a histology certification? > > CAP requires us to: > > 1. List the types of specimens that the non-pathologist is able to do. > 2. State whether the nature of the pathologist supervision is direct or > indirect. > 3. Have a system to evaluate gross descriptions of non-pathologists. > > thanks for your help. > > James R. Vickroy BS, HT (ASCP) > Technical Supervisor, Surgical Pathology > 788-4046 > vickroy.jim@mhsil.com > > > > This E-Mail and any attachments are intended solely for the addressee(s) and > may be legally privileged and /or confidential. If you believe you received > this E-Mail in error, please forward it to mhsil@mhsil.com to notify us of > the error, then delete it; do not divulge, copy, forward or use the contents > in any way. Please be aware that any unauthorized use or disclosure of the > contents of this E-Mail may be unlawful. > > Memorial Health System > 701 N. First Street > Springfield, IL 62781-0001 > 217-757-7753 > http://www.mhsil.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mdcchowa <@t> nus.edu.sg Tue Jan 13 21:52:36 2004 From: mdcchowa <@t> nus.edu.sg (Chow Wai Lyn, Adeline) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] PGP 9.5 Message-ID: <0DA086269B2D1A41AA6714CA61D08F641DDE10@MBXSRV03.stf.nus.edu.sg> Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin taken from the hypothenar region. The results have been inconsistent so far , sometimes staining well, sometimes not. Sections are 30um and stained overnight room temp. using DAKO envision kit. Any advice ? Thanks.. Lyn Research Assistant National University of Singapore From int09018 <@t> alphahunt.com Wed Jan 14 00:29:53 2004 From: int09018 <@t> alphahunt.com (HCS) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] I have a LX-120 tissue processor (non-working) free to anyone who wants it. Message-ID: <000a01c3da67$d20f2380$6501a8c0@hp> I would like to give away my old LX-120, It has a few minor problems so it is not running. It is complete with manuals and extra parts. Let me know if you are interested. LeRoy Brown HT(ASCP) HTL Histology Consultation Services 207 N. Harkness St. Everson, WA 98247 1-800-732-8158 www.histocs.com From IKirbis <@t> onko-i.si Wed Jan 14 01:19:33 2004 From: IKirbis <@t> onko-i.si (=?iso-8859-2?Q?Kirbi=B9_Srebotnik_Irena?=) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] pepsin Message-ID: Dear histoneters, we used to use Pepsin (porcine) from Serva:1 milliAnson U/mg, EC 3.4 23 1, Mr ca. 36 000 which is no more available, is someone familiar with this activity unit for pepsin?, what kind of pepsin can we use instead? treatment with pepsin is a part of FNAB sample processing procedure for the DNA measurements by flow cytometer. thanks for help Irena Kirbis Cytopathology Department Institute of Oncology From kemlo <@t> tiscali.co.uk Wed Jan 14 03:06:41 2004 From: kemlo <@t> tiscali.co.uk (Kemlo Rogerson) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Mortuary In-Reply-To: Message-ID: <000001c3da7d$bd9854b0$9bc4e150@KEMLOS> If descriptions of organs and weights etc are dictated to a MTO and recorded on a proforma, can that proforma be taken out of the Mortuary for the details to be typed up in the report? Given that the 'greens' worn in the Autopsy Suite have to be removed after use, then 'outdoor' cloths put on, can anything safely be removed? Even wider than that; what about forms that accompanies biopsy and resection specimens? They have been in the Theatre, then in cut up, then into an Office environment. Is paper bactericidal? Which infections must we be wary of and what is 'best practice'? In my day I remember a Pathologist that used to perch her cigarette on the cadaver. That broke every rule in the book; H&S, passive smoking, etc. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 01270 877625 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts:? ???????????? kemlo@tiscali.co.uk?or kemlo1@btinternet.com? Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kirbi? Srebotnik Irena Sent: 14 January 2004 07:20 To: 'histonet@pathology.swmed.edu' Subject: [Histonet] pepsin Dear histoneters, we used to use Pepsin (porcine) from Serva:1 milliAnson U/mg, EC 3.4 23 1, Mr ca. 36 000 which is no more available, is someone familiar with this activity unit for pepsin?, what kind of pepsin can we use instead? treatment with pepsin is a part of FNAB sample processing procedure for the DNA measurements by flow cytometer. thanks for help Irena Kirbis Cytopathology Department Institute of Oncology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renatapechova <@t> hotmail.com Wed Jan 14 04:38:51 2004 From: renatapechova <@t> hotmail.com (renata pechova) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] unsubcribe Message-ID: Unsubcribe Renata Pechova PRAHA, Czech republic _________________________________________________________________ Online hry! Spousta zabavy s MSN Messenger 6.1 http://www.msn.cz/procmessenger From hamilt_l <@t> hotmail.com Wed Jan 14 06:02:44 2004 From: hamilt_l <@t> hotmail.com (Loriann Hamilton) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Looking for Lawerence A. -Jim needs to get ahold of you Message-ID: Hello Lawerence please give Jim a call at the lab he needs your contact info. Thanks! Hope all is good in the warm South, becuase its cold up North-haha _________________________________________________________________ There are now three new levels of MSN Hotmail Extra Storage! Learn more. http://join.msn.com/?pgmarket=en-us&page=hotmail/es2&ST=1 From Loralee_Gehan <@t> URMC.Rochester.edu Wed Jan 14 07:02:45 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Osteoclast stain Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804AF@exmc3.urmc.rochester.edu> I do a TRAP stain, the enzyme histochemical way just about everyday and all of my samples are fixed in formalin. For the plastic sections (haven't been done in some time) we fixed in an alcoholic formalin. I can send a protocol if needed. It's not a kit but it is very similar. The antibody I have search high and low for and all I can find is one that works wonderfully in human tissue, not in animal. Hope that helps a little. Loralee Gehan University of Rochester Orthopaedics Research Lab > ---------- > From: Patsy Ruegg > Sent: Tuesday, January 13, 2004 12:25 PM > To: Paul Keogh; 'Histonet (E-mail) > Subject: RE: [Histonet] Osteoclast stain > > Paul, > If you have not fixed with formalin you could do Acid Phosphatase (TRAP) > for > osteoclasts, this is the enzyme histochemical method. I can provide you > the > method if you like. I used a kit from Sigma, I hope someone is still > selling it. There is also an antibody but I do not have experience with > it. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Paul > Keogh > Sent: Tuesday, January 13, 2004 5:33 AM > To: 'Histonet (E-mail) > Subject: [Histonet] Osteoclast stain > > > > > I have sections of MMA embedded rat femur . I want to stain for > osteoclasts. > > Can anyone recommend a tried and tested protocol please.? > > Paul > > > ********************************************************************** > This email is confidential and intended solely for the use > of the individual to whom it is addressed. > Any views or opinions presented are solely those > of the author and do not necessarily represent those > of the Dublin Dental School and Hospital. If you are not > the intended recipient, be advised that you have > received this email in error and that any use, > dissemination, forwarding, printing or copying of this > email is strictly prohibited. If you have received this > email in error, please contact the sender. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From HornHV <@t> archildrens.org Wed Jan 14 08:58:55 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: I have been looking for one of these as well. I have had no luck in finding one. The new ones all seem to be computer based and that is NOT what we want. Please reply to the list. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Regnault, Raoul [mailto:Raoul.Regnault@interiorhealth.ca] Sent: Tuesday, January 13, 2004 3:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From cgin <@t> pen.eiu.edu Wed Jan 14 09:02:04 2004 From: cgin <@t> pen.eiu.edu (ikenwosu) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Seeking used cryostats Message-ID: <400559ec.36e.5c35.23605@pen.eiu.edu> Hi All, Can anypne advise me and how and where to get a used cryostat. My Prof. just got funding to buy a used cryostat for our department and he has given me the responsibilty of purchasing one ASAP. I would appreciate your invaluable contributions! Theodore Ike Nwosu Grad. Std. (MSc.) Eastern Illinois University www.eiu.edu From ASelf <@t> gmhsc.com Wed Jan 14 09:09:00 2004 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] microprobe staining system Message-ID: I have a microprobe staining system that we are getting rid of - if anyone is interested. The last time it was used it worked fine. Amy Self Georgetown Memorial Hospital 843-527-7179 Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From philippegannon <@t> hotmail.com Wed Jan 14 09:10:07 2004 From: philippegannon <@t> hotmail.com (Philippe Gannon) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] unsubcribe Message-ID: Unsubcribe _________________________________________________________________ Add photos to your e-mail with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=features/photos&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From juan.gutierrez <@t> christushealth.org Wed Jan 14 09:22:15 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Work load Message-ID: They used the wrong prefix. Your lab is UNDERstaffed. Good luck, Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: eileen dusek [mailto:eileen_dusek@yahoo.com] Sent: Tue 1/13/2004 2:45 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Work load Hello everyone, A Pathologists was told by the "bean crunchers" they were overstaffed. What is your opinion? The lab does approximately 22,000 cases, Immunos on the Benchmark and a few Special Stains. The gross room is covered 95% of the time by Support Techs. This work load is performed by 2 FTE's and 1 Bench Supervisor. As we know the supervisor cannot bench all the time. Thanks Eileen Dusek --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Jan 14 09:28:18 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: Dictaphone has a nice foot pedal system. The only time the pathologist uses his/her hands on it is to dial their respective log-in credentials at the beginning, and to send the dictation at the end. If they ever figure out that the gross tech can do that for them, they wont ever have to touch it again. Good luck, Juan -----Original Message----- From: Regnault, Raoul [mailto:Raoul.Regnault@interiorhealth.ca] Sent: Tue 1/13/2004 3:22 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Wed Jan 14 09:19:03 2004 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] thank you regarding particle counting Message-ID: <005001c3dab1$bf5d86b0$41693442@selfuggnbwd3wt> Good morning Alton, Anila, Barry, Fred, Jim, and Patty, I would like to thank all of you who responded to my query regarding particle counting. Since this is not my forte, all of the suggestions and contact information as been forwarded to my client. Thank you all again for taking time out of your busy days to help us out. Have a super week!! Cathy ********************************************************** "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com From bjhighto <@t> utmb.edu Wed Jan 14 09:20:23 2004 From: bjhighto <@t> utmb.edu (Hightower, Barbara J.) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] unsubscribe Message-ID: <306666F3E0CDCC46BEE1FA50E877CCB4088E42@EXCHANGE2K8.utmb.edu> From matalikja <@t> upmc.edu Wed Jan 14 09:52:28 2004 From: matalikja <@t> upmc.edu (Matalik, Jennifer) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] articular cartridge Message-ID: Hi, I am currently looking for a special stain that will stain articular cartridge. Does anyone have any suggestions? Jennifer A. Matalik Histology Lab manager 100 technology drive room 271 Pittsburgh, PA 15219 Phone# 412-235-5215 Fax# 412-235-5110 matalikja@upmc.edu From Cathy.Stevens <@t> HealthONEcares.com Wed Jan 14 10:18:43 2004 From: Cathy.Stevens <@t> HealthONEcares.com (Stevens Cathy) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: We just installed the Lanier dictation system. It is used for Radiology and Pathology. All by foot pedal in pathology. For those that want, a hand held microphone is available. It is an awesome system and the support for setting up was wonderful. We can dictate from three outside sites and others by telephone. We have mic's on the doctor's scopes and they operate a foot pedal for dictation. Look them up on the web at www.lanierhealthcare.com -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, January 14, 2004 7:59 AM To: 'Regnault, Raoul'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Standalone Dictation System I have been looking for one of these as well. I have had no luck in finding one. The new ones all seem to be computer based and that is NOT what we want. Please reply to the list. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Regnault, Raoul [mailto:Raoul.Regnault@interiorhealth.ca] Sent: Tuesday, January 13, 2004 3:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Cathy.Stevens <@t> HealthONEcares.com Wed Jan 14 10:21:31 2004 From: Cathy.Stevens <@t> HealthONEcares.com (Stevens Cathy) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: Oops, I see all computer based is not what you want. Can I ask why not? Certainly is user friendly. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, January 14, 2004 7:59 AM To: 'Regnault, Raoul'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Standalone Dictation System I have been looking for one of these as well. I have had no luck in finding one. The new ones all seem to be computer based and that is NOT what we want. Please reply to the list. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Regnault, Raoul [mailto:Raoul.Regnault@interiorhealth.ca] Sent: Tuesday, January 13, 2004 3:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.P.Whitaker <@t> uth.tmc.edu Wed Jan 14 10:38:08 2004 From: Bonnie.P.Whitaker <@t> uth.tmc.edu (Bonnie P Whitaker) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System In-Reply-To: Message-ID: Where are you located? The reason I ask is that I need a system for the lab I will begin work for in 3 weeks, and Lanier hasn't even returned our calls requesting information!! Bonnie Whitaker Houston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stevens Cathy Sent: Wednesday, January 14, 2004 10:19 AM To: 'Horn, Hazel V'; 'Regnault, Raoul'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Standalone Dictation System We just installed the Lanier dictation system. It is used for Radiology and Pathology. All by foot pedal in pathology. For those that want, a hand held microphone is available. It is an awesome system and the support for setting up was wonderful. We can dictate from three outside sites and others by telephone. We have mic's on the doctor's scopes and they operate a foot pedal for dictation. Look them up on the web at www.lanierhealthcare.com -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, January 14, 2004 7:59 AM To: 'Regnault, Raoul'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Standalone Dictation System I have been looking for one of these as well. I have had no luck in finding one. The new ones all seem to be computer based and that is NOT what we want. Please reply to the list. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Regnault, Raoul [mailto:Raoul.Regnault@interiorhealth.ca] Sent: Tuesday, January 13, 2004 3:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSTULTS <@t> fremontmemorial.org Wed Jan 14 11:07:24 2004 From: DSTULTS <@t> fremontmemorial.org (Deb Stults) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] calculators and the HT exam Message-ID: You are able to bring calculators with you, but they have to be checked by the test administrator. The testing site should have calculators and wipe off boards that they let you use during the test. The place I went had everything I needed. From garygill <@t> dcla.com Wed Jan 14 11:12:17 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: And if the tooth is a healthy one, the solution is one normal molar. Gary Gill -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Tuesday, January 13, 2004 6:40 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] normal and molar solutions What do you call a tooth in a glass of water? A one molar solution. From: http://www.coolscience.org/CoolScience/CoolJokes/ChemJokes.htm Tim Morken -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Tuesday, January 13, 2004 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] normal and molar solutions OK IM CONFUSED.......can someone please explain to me how this works....i can't seem to calculate the correct answers if you have time maybe you can talk me through this one problem on finding the molar solution and the normal solution...HERE IT GOES.... this is the question.... How much of each of the following chemicals is needed to prepare 1000 mL of both a 1M and a 1N solution?... Atomic Weights: Na=22.99, Cl=35.45 1. NaCl Thanks a bunch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakerj <@t> umich.edu Wed Jan 14 11:14:46 2004 From: bakerj <@t> umich.edu (John Baker) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] stain for osteoblasts Message-ID: Does anyone have a protocol for something like this? Specimen cells are fixed in 10% aqueous formaldehyde, serially dehydrated, the specimens are embedded in paraffin and cut into 5um thick cross sections for H&E or von Kossa silver nitrate/alizarin red staining. The question is if anyone has a more detailed protocol for staining for bone formation with osteoblasts fixed inside a polymer (alginate in this case). The protocol is wanted by a graduate student in our lab and I am unfamiliar with working with cells. Thank you for any assistance with this! John -- ------------------------------------------------------- Today is the Tomorrow you worried about Yesterday. Was it worth it? Birthdays are good for you. Statistics show that the people who have the most live the longest. ------------------------------------------------------- John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 400 North Ingalls, G160 Ann Arbor, MI 48109-0486 Main lab office phone:734-763-9674 Histology office:734-936-1635 From asmith <@t> mail.barry.edu Wed Jan 14 11:40:54 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] PGP 9.5 Message-ID: <494304423C63E246A5CF87A3AEEB577011B5C5@bumail01.barrynet.barry.edu> I have had very good results staining nerve fibers with Kiernan's physically developed silver method [Kiernan, HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., pp371-373]. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chow Wai Lyn, Adeline Sent: Tuesday, January 13, 2004 10:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PGP 9.5 Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin taken from the hypothenar region. The results have been inconsistent so far , sometimes staining well, sometimes not. Sections are 30um and stained overnight room temp. using DAKO envision kit. Any advice ? Thanks.. Lyn Research Assistant National University of Singapore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Cathy.Stevens <@t> HealthONEcares.com Wed Jan 14 11:41:46 2004 From: Cathy.Stevens <@t> HealthONEcares.com (Stevens Cathy) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Standalone Dictation System Message-ID: We are in Denver/Aurora Colorado. Our account manager is Bill Landreneau. His phone is 1-720-981-4130 or 1-866-856-3000 X 3447. We got ours set up quite fast as the Radiology system died and we had ours budgeted. Maybe it's the manager that we have that was so good. Anyway, we are still satisfied. Cathy -----Original Message----- From: Bonnie P Whitaker [mailto:Bonnie.P.Whitaker@uth.tmc.edu] Sent: Wednesday, January 14, 2004 9:38 AM To: Stevens Cathy; 'Horn Hazel V'; 'Regnault Raoul'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Standalone Dictation System Where are you located? The reason I ask is that I need a system for the lab I will begin work for in 3 weeks, and Lanier hasn't even returned our calls requesting information!! Bonnie Whitaker Houston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stevens Cathy Sent: Wednesday, January 14, 2004 10:19 AM To: 'Horn, Hazel V'; 'Regnault, Raoul'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Standalone Dictation System We just installed the Lanier dictation system. It is used for Radiology and Pathology. All by foot pedal in pathology. For those that want, a hand held microphone is available. It is an awesome system and the support for setting up was wonderful. We can dictate from three outside sites and others by telephone. We have mic's on the doctor's scopes and they operate a foot pedal for dictation. Look them up on the web at www.lanierhealthcare.com -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, January 14, 2004 7:59 AM To: 'Regnault, Raoul'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Standalone Dictation System I have been looking for one of these as well. I have had no luck in finding one. The new ones all seem to be computer based and that is NOT what we want. Please reply to the list. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Regnault, Raoul [mailto:Raoul.Regnault@interiorhealth.ca] Sent: Tuesday, January 13, 2004 3:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standalone Dictation System The pathologists here are looking for a dictation system that allows all dictation functions to be accessed through a foot pedal. Our current system has been onsite modified to allow the concurrent use of the foot pedal with a dictation microphone while they are prosecting. This has the disadvantage that a switch on the microphone needs to be worked to change from the dictation mode to transcription mode (and vice versa) to allow review and editing of the macroscopic dictation. They find this inefficient and cumbersome. The pathologists would also like to use a foot pedal and boom microphone while dictating microscopics instead of a hand microphone. All stand alone dictating dictating systems seem to expect foot pedals and headsets to be used for transcription only and handheld microphones only to be used for dictation. I believe some central and digital systems allow foot pedal and mic but they are not an option at this time. Does any one on the list have experience, comments or pointers? Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Wed Jan 14 12:12:37 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Seeking used cryostats In-Reply-To: <400559ec.36e.5c35.23605@pen.eiu.edu> References: <400559ec.36e.5c35.23605@pen.eiu.edu> Message-ID: <40058695.6080000@bitstream.net> Contact: Ford Royer Analytical Instruments, LLC 1200 Mendelssohn Ave. N., Ste. 50 Minneapolis, MN 55427-4366 (800) 565-1895, Ext. 17 Fax: 952-929-1895 ikenwosu wrote: >Hi All, > Can anypne advise me and how and where to get a used >cryostat. My Prof. just got funding to buy a used cryostat >for our department and he has given me the responsibilty of >purchasing one ASAP. > I would appreciate your invaluable contributions! > >Theodore Ike Nwosu >Grad. Std. (MSc.) >Eastern Illinois University >www.eiu.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From garygill <@t> dcla.com Wed Jan 14 12:18:14 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Seeking used cryostats Message-ID: Try Tom O'Brien, IMEB (International Medical Equipment Brokers [New & Reconditioned Pathology Lab Equipment]), at (800) 543-8496 (http://www.imebinc.com/). Gary Gill -----Original Message----- From: Ford Royer [mailto:froyer@bitstream.net] Sent: Wednesday, January 14, 2004 1:13 PM To: cgin@eiu.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Seeking used cryostats Contact: Ford Royer Analytical Instruments, LLC 1200 Mendelssohn Ave. N., Ste. 50 Minneapolis, MN 55427-4366 (800) 565-1895, Ext. 17 Fax: 952-929-1895 ikenwosu wrote: >Hi All, > Can anypne advise me and how and where to get a used cryostat. My >Prof. just got funding to buy a used cryostat for our department and he >has given me the responsibilty of purchasing one ASAP. > I would appreciate your invaluable contributions! > >Theodore Ike Nwosu >Grad. Std. (MSc.) >Eastern Illinois University >www.eiu.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Jan 14 12:21:11 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Re: articular cartridge Staining In-Reply-To: Message-ID: <3.0.6.32.20040114112111.00bcffd8@gemini.msu.montana.edu> You did not say how the articular cartilage is prepared? Frozen sections, paraffin, decalcified, plastic sections. Toluidine Blue or saffranin O/Fast Green work well with paraffin. Toluidine blue is excellent with plastic sections. IF you have decalcified with EDTA, proteoglycans are extracted (NOT destroyed) by EDTA and tinctorial quality of these stains changes. You will need to control staining using an undecalcified piece of articular cartilage so you know what might be missing. At 10:52 AM 1/14/2004 -0500, you wrote: >Hi, I am currently looking for a special stain that will stain articular >cartridge. Does anyone have any suggestions? > >Jennifer A. Matalik >Histology Lab manager >100 technology drive >room 271 >Pittsburgh, PA 15219 >Phone# 412-235-5215 >Fax# 412-235-5110 >matalikja@upmc.edu > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From kaburns <@t> med.unc.edu Wed Jan 14 12:33:09 2004 From: kaburns <@t> med.unc.edu (Kim Burns) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Lab safety Message-ID: <000501c3dacc$dc62ec80$0b2e1398@peds.med.unc.edu> My question has to do with the inspection of a medical examiner's histopathology laboratory. What agencies or organizations are responsible for inspecting these labs? Are these inspections necessary for these labs to operate legally. Thanks for the information. Kimberlie Burns C.F. Center UNC-Chapel Hill. kaburns@med.unc.edu From sohaskey <@t> socrates.Berkeley.EDU Wed Jan 14 12:36:40 2004 From: sohaskey <@t> socrates.Berkeley.EDU (Mike Sohaskey) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] folds forming in bone/cartilage sections Message-ID: Hello, I find that, when sectioning EDTA-decalcified mouse tibiae at 5 - 10 microns, that folds (i.e., creases) frequently form specifically within the growth plate cartilage as the sections dry and adhere to the Superfrost slides. I have had no success with increasing the amount of time that I float the sections on a water bath prior to drying them on slides. I'm hoping that someone might be able to offer a suggestion as to how I can overcome this difficulty. Thanks very much. MIKE Michael Sohaskey, Ph.D. University of California, Berkeley Molecular and Cell Biology 585 Life Sciences Addition Berkeley, CA 94720-3200 sohaskey@socrates.berkeley.edu (510) 643-2775 (phone) From lizchlipala <@t> premierhistology.com Wed Jan 14 12:58:11 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] folds forming in bone/cartilage sections In-Reply-To: Message-ID: <000001c3dad0$5e6d9b00$74d48a80@LIZ> Mike We place all of our bone sections on a hot plate (around 40 degrees C, the paraffin should not melt) overnight and then stain them. If you need to take a look at some images you can go to our web page, there should be some images in the gallery on mouse joints, and a few pdf files that you could down load that might be helpful. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Sohaskey Sent: Wednesday, January 14, 2004 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] folds forming in bone/cartilage sections Hello, I find that, when sectioning EDTA-decalcified mouse tibiae at 5 - 10 microns, that folds (i.e., creases) frequently form specifically within the growth plate cartilage as the sections dry and adhere to the Superfrost slides. I have had no success with increasing the amount of time that I float the sections on a water bath prior to drying them on slides. I'm hoping that someone might be able to offer a suggestion as to how I can overcome this difficulty. Thanks very much. MIKE Michael Sohaskey, Ph.D. University of California, Berkeley Molecular and Cell Biology 585 Life Sciences Addition Berkeley, CA 94720-3200 sohaskey@socrates.berkeley.edu (510) 643-2775 (phone) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCarpenter764 <@t> aol.com Wed Jan 14 13:39:57 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: Fwd: [Histonet] normal and molar solutions Message-ID: ok i think i know the gist of this now......but i am still confused on how to get the positive valence, or number of dissociable or replacemable hydrogen ion...how do i figure this out. From JCarpenter764 <@t> aol.com Wed Jan 14 13:53:41 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal solutions question Message-ID: Thank You guys for all the responses i have received...but im still STUCK i don't understand how to come up with the positive valence, or number of dissociable or replaceable hydrogen ions.....this is the number that i divide the molar weight with to get the normal weight....every problem i have worked out i seem to be dividing by 2 and that doesn't make since to me. From BRobert <@t> ameripath.com Wed Jan 14 13:54:57 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Seeking used cryostats Message-ID: Try Recycle.net/used-equip/Laboratory-Equip/ they have used, refurbished lab equipment. -----Original Message----- From: ikenwosu [mailto:cgin@pen.eiu.edu] Sent: Wednesday, January 14, 2004 7:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking used cryostats Hi All, Can anypne advise me and how and where to get a used cryostat. My Prof. just got funding to buy a used cryostat for our department and he has given me the responsibilty of purchasing one ASAP. I would appreciate your invaluable contributions! Theodore Ike Nwosu Grad. Std. (MSc.) Eastern Illinois University www.eiu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Wed Jan 14 13:58:39 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: This link explains it all: http://www.uab.edu/clabsc/solution.htm BTW, molar means "little pile". Gary Gill -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Wednesday, January 14, 2004 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: Fwd: [Histonet] normal and molar solutions ok i think i know the gist of this now......but i am still confused on how to get the positive valence, or number of dissociable or replacemable hydrogen ion...how do i figure this out. From northma <@t> ohsu.edu Wed Jan 14 14:03:20 2004 From: northma <@t> ohsu.edu (Mary North) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Bone saws Message-ID: We are looking for a mechanized saw to section femurs. We have the SawBones and stryker available but would prefer a moving blade through which we can safely move the specimen. Do any of you use the Buehler Isomet? Are you using other types of saws? I appreciate your feedback. Mary North, HT(ASCP), HTL Oregon Health & Science University Portland, OR From gcallis <@t> montana.edu Wed Jan 14 14:05:09 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] folds forming in bone/cartilage sections In-Reply-To: Message-ID: <3.0.6.32.20040114130509.00bcdf98@gemini.msu.montana.edu> Two things that may help One, be careful about overprocessing mouse bone, decalcified tibia need only 1 holur per change, with this schedule and hopefully you have vacuum and pressure. 70%, 80%, 95% X 2, 100% x 2, xylene X 1, Clearite 3 X 1, paraffin a minimum of 3 chnages (30, 30, 1 hour) Use harder paraffin to give more rigidity to support embedding media, Tissue Prep 2 is excellent or at least embed in this. Do not add heat to processing and make sure the paraffin is no hotter than 60C. If you remove too much of bound water from cartilage rather than free water in tissue spaces, you will have problems. What you are dealing with is softer growth plate cartilage surrounded by much harder bone. Soaking helps the harder decalcified bone soften while cartilage is picking up some water. Don't oversoak or bone will swell out of block = messy sections! Trim block, soak on ice block with water on top of ice for a few minutes, cut at 5 um using a really GOOD quality disposable blade, and lay ribbon on a waterbath containing approx 5% DMSO! Pick up section on plus charge slide, drain well and dry overnight at 37C. If your waterbath is too hot, wrinkles set and you cannot get them corrected. Adjust waterbath carefully. DMSO lowers the surface tension of water, and allows cartilage to stretch properly without having to pull on the paraffin ribbon. Your ribbon must be totally uncompressed and wrinkled to begin with and that requires the best blade. I have excellent luck with these knives, Dura Edge, AccueEdge and EdgeRite. We also prefer high profile for more stability, edges are as sharp as low profiles. 5 um sections are preferred, 10 um may give you more wrinkle problems. Low profile blades will work as long as the bones are perfectly processed. Good luck, At 10:36 AM 1/14/2004 -0800, you wrote: >Hello, > > I find that, when sectioning EDTA-decalcified mouse tibiae at >5 - 10 microns, that folds (i.e., creases) frequently form >specifically within the growth plate cartilage as the sections dry >and adhere to the Superfrost slides. I have had no success with >increasing the amount of time that I float the sections on a water >bath prior to drying them on slides. I'm hoping that someone might >be able to offer a suggestion as to how I can overcome this >difficulty. Thanks very much. > >MIKE > >Michael Sohaskey, Ph.D. >University of California, Berkeley >Molecular and Cell Biology >585 Life Sciences Addition >Berkeley, CA 94720-3200 >sohaskey@socrates.berkeley.edu >(510) 643-2775 (phone) > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Jan 14 14:14:12 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:25 2005 Subject: Reading this with interest RE: [Histonet] normal and molar solutions In-Reply-To: Message-ID: <3.0.6.32.20040114131412.00bcdf98@gemini.msu.montana.edu> Thanks to Gary for handy website. Hrapchak and Sheehans histotechnics books and plus other histobooks tell you how to calculate these solutions and often have charts telling normality and molarity for common chemicals. If this helps, use it! My physical chemist husband suggested that anytime I write up a protocol or publication requiring normal solutions, that I convert these to molar solutions. This is what he does, and it is so much simpler for everyone! Ahh the joys of basic chemistry! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From tpmorken <@t> labvision.com Wed Jan 14 14:32:06 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:25 2005 Subject: Reading this with interest RE: [Histonet] normal and molar so lutions Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20356F8@usca0082k08.labvision.apogent.com> Gayle, Your husbands comments are right inline with what I found in when I looked around the web a bit and found that the term "normal solution" is out of favor now. I was wondering if the ASCP tests should be modified to be up to date. Chemistry chapter http://www.iupac.org/publications/analytical_compendium/Cha06sec3.pdf Use of terms "normal solution" and "normality" are not recommended. Chem website http://home.att.net/~cat6a/electrolysis-III.htm "Since normal solutions are a bit confusing, these days the practice is to quote strength of solutions in molar terms." Tim Morken Lab Vision / Neomarkers www.labvision.com -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, January 14, 2004 12:14 PM To: Histonet@lists.utsouthwestern.edu Subject: Reading this with interest RE: [Histonet] normal and molar solutions Thanks to Gary for handy website. Hrapchak and Sheehans histotechnics books and plus other histobooks tell you how to calculate these solutions and often have charts telling normality and molarity for common chemicals. If this helps, use it! My physical chemist husband suggested that anytime I write up a protocol or publication requiring normal solutions, that I convert these to molar solutions. This is what he does, and it is so much simpler for everyone! Ahh the joys of basic chemistry! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Wed Jan 14 14:33:10 2004 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Bone saws Message-ID: <8F3AB322628548428A992EFB0E80F5D3A2EED4@nihexchange8.nih.gov> I use the table saw from Mar-Med Inc. the phone number is 440-572-5175 address 14554 Windsor Castle Ln. Cleveland Ohio 44149. I think the website is Mar-Med.com. It's great takes up little space and is a real workhorse. I have had someone cut rabbit femurs with muscle tissue attached and it worked great. I also use the Buehler Isomet for finer cutting. The Mar-Med is best for the bone and it's inexpensive. Ruth Yaskovich National Institutes of Dental and Craniofacial Research N.I.H. 301-496-1392 -----Original Message----- From: Mary North [mailto:northma@ohsu.edu] Sent: Wednesday, January 14, 2004 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone saws We are looking for a mechanized saw to section femurs. We have the SawBones and stryker available but would prefer a moving blade through which we can safely move the specimen. Do any of you use the Buehler Isomet? Are you using other types of saws? I appreciate your feedback. Mary North, HT(ASCP), HTL Oregon Health & Science University Portland, OR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jenny-Oblander <@t> omrf.ouhsc.edu Wed Jan 14 14:33:46 2004 From: Jenny-Oblander <@t> omrf.ouhsc.edu (Jenny Oblander) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: This page has come in handy, especially the conversion calculator. Jenny www.manuelsweb.com -----Original Message----- From: Gary Gill [mailto:garygill@dcla.com] Sent: Wednesday, January 14, 2004 1:59 PM To: 'JCarpenter764@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] normal and molar solutions This link explains it all: http://www.uab.edu/clabsc/solution.htm BTW, molar means "little pile". Gary Gill -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Wednesday, January 14, 2004 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: Fwd: [Histonet] normal and molar solutions ok i think i know the gist of this now......but i am still confused on how to get the positive valence, or number of dissociable or replacemable hydrogen ion...how do i figure this out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtsonger <@t> vt.edu Wed Jan 14 14:54:23 2004 From: jtsonger <@t> vt.edu (Jill Songer) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Fraser-Lendrum stain Message-ID: <6.0.0.22.0.20040114155253.01bb07f0@pop.vt.edu> Would anybody in Histoland mind sharing their protocol for this fibrin stain with me? Many thanks. Jill Songer HT (ASCP) Histopathology Lab Veterinary Medical Teaching Hospital Virginia Tech From jmitchell <@t> neurology.wisc.edu Wed Jan 14 14:51:53 2004 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean A.)) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] PGP 9.5 Message-ID: <061D5F3CF2DFDF4EADF5611CC1218C4512A667@nrl-lorenz.neurology.wisc.edu> I have been using a polyclonal PGP 9.5 for immunolabeling epidermal neurites on 50um frozen sections of skin. Are you staining sections on slides or free floating your sections in well plates? I have attempted both and by far the most consistant results are obtained by free floating the sections in well culture plates for the entire staining procedure. Good luck & contact me directly if I can assist you more with this technique. Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI -----Original Message----- From: Chow Wai Lyn, Adeline [mailto:mdcchowa@nus.edu.sg] Sent: Tuesday, January 13, 2004 9:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PGP 9.5 Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin taken from the hypothenar region. The results have been inconsistent so far , sometimes staining well, sometimes not. Sections are 30um and stained overnight room temp. using DAKO envision kit. Any advice ? Thanks.. Lyn Research Assistant National University of Singapore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Jan 14 15:16:47 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:25 2005 Subject: Reading this with interest RE: [Histonet] normal and molar solutions Message-ID: <494304423C63E246A5CF87A3AEEB577011B5C6@bumail01.barrynet.barry.edu> The principal problem with normality is sloppy usage. A solution of xyz can be 1 molar, but it cannot be just plain 1 normal: it must be 1 normal in x or y or z. Normality is the concentration of the reactive species. Normality is a useful concept if it is expressed correctly. Allen A. Smith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 14, 2004 3:14 PM To: Histonet@lists.utsouthwestern.edu Subject: Reading this with interest RE: [Histonet] normal and molar solutions Thanks to Gary for handy website. Hrapchak and Sheehans histotechnics books and plus other histobooks tell you how to calculate these solutions and often have charts telling normality and molarity for common chemicals. If this helps, use it! My physical chemist husband suggested that anytime I write up a protocol or publication requiring normal solutions, that I convert these to molar solutions. This is what he does, and it is so much simpler for everyone! Ahh the joys of basic chemistry! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From FreidaC <@t> aol.com Wed Jan 14 15:19:25 2004 From: FreidaC <@t> aol.com (FreidaC@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal solutions question Message-ID: <39.42d641f9.2d370c5d@aol.com> Unless you are taking the HTL exam, you should not worry about normal solutions. Questions were not asked about normal solutions in the past - only questions on molar solutions. The exam may have changed, but you would only get one or two questions at the most. I would not concern myself too much about that. If you have had chemistry, then you should not have a problem with normal or molar solutions, and if you have not had chemistry, then you cannot be taking the HTL exam - so do not worry about normal solutions, only molar solutions. Freida Carson From asmith <@t> mail.barry.edu Wed Jan 14 15:43:55 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal solutions question Message-ID: <494304423C63E246A5CF87A3AEEB577011B5C7@bumail01.barrynet.barry.edu> Normality is the concentration of the reactive species. If the reactive species is hydrogen ions, you have to look up (or memorize) the number of hydrogen ions per molecule of acid. If someone gives the formula of lactic acid as CH3CHOHCOOH, you are supposed to know that only the last hydrogen dissociates in water and reacts with hydroxide ions. Thus, 1 molar lactic acid is 1 normal. If the formula of lactic acid is given as C3H6O3, you have to look up the structural formula. Sulfuric acid is H2SO4. There are two dissociable hydrogens. Thus, 1 molar sulfuric acid is 2 normal (or, better, 2 normal in hydrogen ions). Phosphoric acid is H3PO4. There are three dissociable hydrogens. Thus 1 molar phosphoric acid is 3 normal in hydrogen ions. Citric acid also has 3 dissociable hydrogens, and 1 molar citric acid is 3 normal in hydrogen ions. The molecular weight is 192 daltons per molecule (or 192 grams per mole). 192 grams of citric acid in a liter of solution would be a 1 molar or 3 normal solution. If you want a 1 normal solution, you will need only 1/3 as much citric acid per liter. Thus, 64 grams of citric acid in a liter of solution would be 0.333 molar or 1 normal, and 64 would be the "normal weight" of citric acid (assuming that you are concerned with the normality of dissociable hydrogen, which is usually the case). Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JCarpenter764@aol.com Sent: Wednesday, January 14, 2004 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] normal solutions question Thank You guys for all the responses i have received...but im still STUCK i don't understand how to come up with the positive valence, or number of dissociable or replaceable hydrogen ions.....this is the number that i divide the molar weight with to get the normal weight....every problem i have worked out i seem to be dividing by 2 and that doesn't make since to me. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From froyer <@t> bitstream.net Wed Jan 14 15:50:46 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Bone saws In-Reply-To: <8F3AB322628548428A992EFB0E80F5D3A2EED4@nihexchange8.nih.gov> References: <8F3AB322628548428A992EFB0E80F5D3A2EED4@nihexchange8.nih.gov> Message-ID: <4005B9B6.5060601@bitstream.net> In my lab days, we used a Sears Craftsman bench top band saw that one of the P.A.s bought at a garage sale. But then, that was in the old days when there was never any money to buy new equipment for the Histology Lab. Times are different now. Right? ;-) Never the less it worked great and was not difficult to disinfect. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, LLC Minneapolis, MN Yaskovich, Ruth A (NIH/NIDCR) wrote: >I use the table saw from Mar-Med Inc. the phone number is 440-572-5175 >address 14554 Windsor Castle Ln. Cleveland >Ohio 44149. I think the website is Mar-Med.com. It's great takes up little >space and is a real workhorse. I have had someone cut rabbit femurs with >muscle tissue attached and it worked great. I also use the Buehler Isomet >for finer cutting. The Mar-Med is best for the bone and it's inexpensive. >Ruth Yaskovich >National Institutes of Dental and Craniofacial Research >N.I.H. >301-496-1392 > >-----Original Message----- >From: Mary North [mailto:northma@ohsu.edu] >Sent: Wednesday, January 14, 2004 3:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Bone saws > > >We are looking for a mechanized saw to section femurs. We have the SawBones >and stryker available but would prefer a moving blade through which we can >safely move the specimen. Do any of you use the Buehler Isomet? Are you >using other types of saws? I appreciate your feedback. Mary North, HT(ASCP), >HTL Oregon Health & Science University Portland, OR >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Dndsomi <@t> aol.com Wed Jan 14 15:58:42 2004 From: Dndsomi <@t> aol.com (Dndsomi@aol.com) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Freida Carson's Histotechnology Text Message-ID: <8f.37013716.2d371592@aol.com> Does anyone know if there is a disk available for Freida Carson's Histotechnology, A Self-Instructional Text? Thanks. From bryand <@t> netbistro.com Wed Jan 14 22:49:10 2004 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Fraser-Lendrum stain References: <6.0.0.22.0.20040114155253.01bb07f0@pop.vt.edu> Message-ID: <009f01c3db23$033eee20$bb70c2cf@bryand> Lendrum, Fraser and others published several fibrin stains, but I presume the method you want is the Picro-Mallory, since that is the most well known. There are several variants of the method, all published by Lendrum. Some are more complicated than others. I include two of them here. With this trichrome stain full and complete fixation is absolutely essential. Minimalist formalin fixation and quick processing will give poorly stained erythrocytes with red or orange tinges instead of the yellow they should have. Lendrum and coworkers specified about seven days fixation in formal sublimate (9 parts saturated mercuric chloride and 1 part concentrated formalin) followed by processing thoroughly, sectioning, degreasing with trichlorethylene for 48 hours, then refixing in picromercuric ethanol (ethanol saturated with picric acid and mercuric chloride). Adequate results can be obtained with thorough formalin fixation (24 hours), thorough processing (overnight), sectioning, followed by refixing in Bouin's fluid at 56C for an hour or so. However, results are inferior to those obtained by the full procedure. It is also a method where experience is required for optimal results. I always found the shorter variant quite satisfactory. Long variant (Picro-Mallory IV, I think) Yellow mordant Saturated picric acid in 80% ethanol - 100 mL Orange G - 0.2 g. Lissamine yellow - 0.2 g Stock differentiator picric acid - 2.5 g. 95% ethanol - 100 mL Phosphotungstic acid - 25 g. Dissolve and filter Red stain Acid fuchsin or biebrich scarlet - 1% in 1% acetic acid or Acid fuchsin - 0.5% in 1% acetic acid - 80 mL Lissamine fast red - 1% in 1% acetic acid - 20 mL Red differentiator Stock differentiator - 40 mL 95% ethanol - 20 mL Distilled water - 40 mL Blue stain Soluble or methyl blue - 1% in 1% acetic acid Blue differentiator Stock differentiator - 10 mL Distilled water - 90 mL Method 1. Bring sections to water through xylene and ethanols. Remove mercury pigment. 2. Stain nuclei with an acid resistant nuclear stain (Weigert, Celestine blue hemalum). 3. Place in yellow mordant for 2-3 minutes. 4. Wash in distilled water until only erythrocytes are yellow. 5. Place in the red stain for 5-10 minutes. 6. Rinse with 1% aqueous acetic acid. 7. Differentiate with the red differentiator, microscopically, until fibrin stands out clearly. 8. Rinse well in distilled water. 9. Place in the blue stain for 5 minutes. 10. Rinse briefly with 1% aqueous acetic acid. 11. Place in the blue differentiator for 1-2 minutes. 12. Rinse with 1% aqueous acid briefly. 13. Dehydrate with ethanol, clear with xylene and mount in resinous medium. Results Fibrin - clear red Erythrocytes - yellow Connective tissue - blue Shorter variant (Picro-Mallory V) Picro-orange Orange G - 0.2 g Picric acid saturated in 80% ethanol - 100 mL Yellow differentiator Picro-orange - 50 mL 80% ethanol - 50 mL Red stain Acid fuchsin - 1% in 1% aqueous acetic acid Differentiator 1% phosphotungstic acid in distilled water Blue stain Soluble or methyl blue - 2% in 2% aqueous acetic acid Method 1. Bring sections to water through xylene and ethanols. Remove mercury pigment. 2. Stain nuclei with an acid resistant nuclear stain (Weigert, Celestine blue hemalum). 3. Rinse with 95% ethanol 3. Place in picro-orange for 2 minutes. 4. Without rinsing, place in red stain for 5 minutes. 5. Rinse with distilled water. 6. Dip into yellow differentiator. 7. Rinse with distilled water. 8. Place into phosphotungstic acid solution for 5 minutes. 9. Rinse with distilled water. 10. Stain in blue stain for 2 minutes. 11. Rinse well with 1% aqueous acetic acid. 12. Dehydrate with ethanol, clear with xylene and mount in a resinous medium. Results Fibrin - clear red Erythrocytes - orange Collagen - blue Fibrinoid - varies red to orange Bryan Llewellyn ----- Original Message ----- From: "Jill Songer" To: Sent: Wednesday, January 14, 2004 12:54 PM Subject: [Histonet] Fraser-Lendrum stain > Would anybody in Histoland mind sharing their protocol for this fibrin > stain with me? Many thanks. > > Jill Songer HT (ASCP) > Histopathology Lab > Veterinary Medical Teaching Hospital > Virginia Tech > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From lpwenk <@t> covad.net Thu Jan 15 04:46:29 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal solutions question References: Message-ID: <008201c3db54$d5465d00$30676940@hppav> It is the number of replaceable hydrogen atoms. The charge of one hydrogen atom is +1. So multiply the number of replaceable hydrogen atoms by (+1). In hydrochloric acid, the formula is HCl. There is 1 H, so it is 1 x (+1), so the valence is 1. In sulfuric acid, the formula is H2SO4. (Sorry, can't make my email do subnumbers.). There are 2 replaceable H, so it is 2 x (+1), so the valence is 2. It gets confusing if you are trying to make a normal solution, and the chemical does not have replaceable hydrogen atoms, but does have a positively charged atom. Or if you have a polyatom (atoms that always stay together, like hydroxide (OH) or phosphate (PO4) or carbonate (CO3) or sulfate (SO4)). If the hydrogen is in the polyatom is NOT counted as a replaceable atom. So you have to count the number of positive charges of the non-polyatom. Aluminum hydroxide is Al(OH)3. There is one aluminum atom, with a +3 charge. There are 3 hydroxide (OH), with a charge of -1 each. The hydrogen in the hydroxide is not replaceable. It is "stuck" in the hydroxide. You have to look at the "replaceable" positive charges. So there is 1 Al with a +3 charge, so that is 1 x (+3) = valence of 3. You need to know the charge of the positive atom (from the periodic table or other chemical table) and multiply it by the number of positive atoms in that compound. CaO is 1 calcium x (+2) = 2 valence. K2CO3 is 2 potassium atoms x (+1) = 2 valence. To make this even worse, if you are making a normal solution by diluting a liquid (solute such as HCl or H2SO4) into the water (solvent), the formula becomes even more complicated, as you have to include the concentration (c) of the solute as well as the specific gravity (sp). Both of these figures are usually obtained off the label of the bottle or from the MSDS. (Water has a specific gravity of 1. If the solution is heavier/thicker than water, the sp will be slightly larger than 1.0. If the solution is lighter/runnier than water, the sp will be less than 1.0.) And HCl and H2SO4, for example, are not pure acid. HCl is about a 33-37% solution when it is concentrate, with a specific gravity of about 1.1. If anyone wants the formula of normality for liquids, I'll post that tonight. I have it at work, and don't remember off the top of my head, and have to leave early for work because of the 7 inches of snow that hit us last night. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Wednesday, January 14, 2004 2:53 PM Subject: [Histonet] normal solutions question > Thank You guys for all the responses i have received...but im still STUCK i > don't understand how to come up with the positive valence, or number of > dissociable or replaceable hydrogen ions.....this is the number that i divide the > molar weight with to get the normal weight....every problem i have worked out i > seem to be dividing by 2 and that doesn't make since to me. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rschoon <@t> email.unc.edu Thu Jan 15 06:36:17 2004 From: rschoon <@t> email.unc.edu (rschoon@email.unc.edu) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] precollagen antibody In-Reply-To: References: Message-ID: <1074170177.400689420b7ee@webmail8.isis.unc.edu> Fellow HistoNetter's Has anyone used any antibodies against precollagen in FFPE tissues (that worked)? If so I would appreciate any information you would care to share. TIA Robert Schoonhoven CEHS Univ. of North Carolina From ian.montgomery <@t> bio.gla.ac.uk Thu Jan 15 07:15:47 2004 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Molar and Normal solutions. Message-ID: <6.0.1.1.2.20040115130705.02d3a840@udcf.gla.ac.uk> When I was a boy, before of the dawn of time, I used to make up various Ringer solutions for a character who would add the odd Molal solution to the list of ingredients. He claimed it kept me on my toes while I just thought he was a twisted sod. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From Loralee_Gehan <@t> URMC.Rochester.edu Thu Jan 15 08:24:24 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] folds forming in bone/cartilage sections Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804B4@exmc3.urmc.rochester.edu> We have been using a warming plate to lay our sections on after we cut them. This has helped them flatten out and stick to the plus slides better. Loralee Gehan > ---------- > From: Gayle Callis > Sent: Wednesday, January 14, 2004 3:05 PM > To: Mike Sohaskey; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] folds forming in bone/cartilage sections > > Two things that may help > > One, be careful about overprocessing mouse bone, decalcified tibia need > only 1 holur per change, with this schedule and hopefully you have vacuum > and pressure. > > 70%, 80%, 95% X 2, 100% x 2, xylene X 1, Clearite 3 X 1, paraffin a > minimum > of 3 chnages (30, 30, 1 hour) Use harder paraffin to give more rigidity > to > support embedding media, Tissue Prep 2 is excellent or at least embed in > this. Do not add heat to processing and make sure the paraffin is no > hotter > than 60C. If you remove too much of bound water from cartilage rather > than > free water in tissue spaces, you will have problems. What you are dealing > with is softer growth plate cartilage surrounded by much harder bone. > Soaking helps the harder decalcified bone soften while cartilage is > picking > up some water. Don't oversoak or bone will swell out of block = messy > sections! > > Trim block, soak on ice block with water on top of ice for a few minutes, > cut at 5 um using a really GOOD quality disposable blade, and lay ribbon > on > a waterbath containing approx 5% DMSO! Pick up section on plus charge > slide, drain well and dry overnight at 37C. If your waterbath is too hot, > wrinkles set and you cannot get them corrected. Adjust waterbath > carefully. > > DMSO lowers the surface tension of water, and allows cartilage to stretch > properly without having to pull on the paraffin ribbon. Your ribbon must > be totally uncompressed and wrinkled to begin with and that requires the > best blade. I have excellent luck with these knives, Dura Edge, AccueEdge > and EdgeRite. We also prefer high profile for more stability, edges are > as > sharp as low profiles. 5 um sections are preferred, 10 um may give you > more > wrinkle problems. Low profile blades will work as long as the bones are > perfectly processed. > > Good luck, > > > > At 10:36 AM 1/14/2004 -0800, you wrote: > >Hello, > > > > I find that, when sectioning EDTA-decalcified mouse tibiae at > >5 - 10 microns, that folds (i.e., creases) frequently form > >specifically within the growth plate cartilage as the sections dry > >and adhere to the Superfrost slides. I have had no success with > >increasing the amount of time that I float the sections on a water > >bath prior to drying them on slides. I'm hoping that someone might > >be able to offer a suggestion as to how I can overcome this > >difficulty. Thanks very much. > > > >MIKE > > > >Michael Sohaskey, Ph.D. > >University of California, Berkeley > >Molecular and Cell Biology > >585 Life Sciences Addition > >Berkeley, CA 94720-3200 > >sohaskey@socrates.berkeley.edu > >(510) 643-2775 (phone) > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From syedab <@t> totalise.co.uk Thu Jan 15 08:44:04 2004 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] Periodic acid in immunohistochemistry References: <95774A6A6036D411AFEA00D0B73C8643088804B4@exmc3.urmc.rochester.edu> Message-ID: <003f01c3db76$058f3100$80c401a3@clneuro.ox.ac.uk> I have two protocols for my antibody, 15F6. One which uses 1% periodic acid (and then 1% formic acid) and one which does not have this step. Does anyone know what the periodic acid does in this technique please, and whether it is necessary to include it? I have been trying this technique without success this week hence searching the internet for an alternative protocol. Thanks very much for you kind attention, Anila Syed --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.560 / Virus Database: 352 - Release Date: 08/01/04 From Susan.Walzer <@t> HCAHealthcare.com Thu Jan 15 08:45:07 2004 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Sep 16 15:22:25 2005 Subject: [Histonet] normal and molar solutions Message-ID: <49A0DB96A0A50C46A2D123454348B5E302138FD3@orlex07.orl.medcity.net> The formula for these solutions are in most histology books..we know as we are always having to make up these solutions for our chemistry dept. (ironic, huh?) -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Tuesday, January 13, 2004 5:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] normal and molar solutions OK IM CONFUSED.......can someone please explain to me how this works....i can't seem to calculate the correct answers if you have time maybe you can talk me through this one problem on finding the molar solution and the normal solution...HERE IT GOES.... this is the question.... How much of each of the following chemicals is needed to prepare 1000 mL of both a 1M and a 1N solution?... Atomic Weights: Na=22.99, Cl=35.45 1. NaCl Thanks a bunch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jan 15 09:42:29 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Re: DMSO In-Reply-To: References: <3.0.6.32.20040114130509.00bcdf98@gemini.msu.montana.edu> Message-ID: <3.0.6.32.20040115084229.00bd0638@gemini.msu.montana.edu> Whoops, broke a rule of mine, abbreviation DMSO is Dimethyl sulfoxide, and you can order it from any chemical supplier, it is a solvent commonly used in laboratories. Follow Material Safety Data Sheet instructions on handling this product. > Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Hi Gayle, >kann you tell me what you mean with DMSO and where I kann order this >product? >Jocelyne Leclerc > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From katherine-walters <@t> uiowa.edu Thu Jan 15 09:52:36 2004 From: katherine-walters <@t> uiowa.edu (katherine-walters@uiowa.edu) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] aqueous counterstain for plant tissue Message-ID: <1074181956.4006b7442be32@webmail1.its.uiowa.edu> Dear Plant Gurus, I'm doing an alkaline phosphatase immuno-stain on some plant tissues. My chromogen-substrate solution is fast red, so I can't use any organic solvents or my signal will disappear. I came across malachite green as a plant counterstain, and actually have an old bottle, but I'm not sure how it works and my web search hasn't produced anything very useful so far. Does anyone remember this stain and how it works, or have a better suggestion for a counterstain. Hematoxylin isn't very useful due to the small size of plant nuclei, I imagine a cell wall stain would work best, but most seem to be alcoholic solutions. Thanks a million for any help you can give me! Kathy From gcallis <@t> montana.edu Thu Jan 15 09:57:11 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: More on tricks to prevent RE: [Histonet] folds forming in bone/cartilage sections In-Reply-To: <95774A6A6036D411AFEA00D0B73C8643088804B4@exmc3.urmc.roches ter.edu> Message-ID: <3.0.6.32.20040115085711.00bd0638@gemini.msu.montana.edu> I have done this in the past and exploded my bone sections to the point of no return, it also will put "bubble" artifact aka vacuole in the nuclei of cells IF the hot plate is too warm. Another trick is to have a room temperature waterbath containing 10% ethanol, lay section on that, pick up section, onto a nonplus charge slide, then go to a warm waterbath and slowly lower section onto warmer water, this will flatten sections. In a pinch, I have also put a few drops of 10% under the section after picking it up, then laying it on a warm water bath to flatten. Most of the time, a perfectly processed piece of bone has few to no problems and you get the perfectly flat section with a very sharp disposable blade. Have been known to change blade edges between each ribbon IF problems occur. The price of a blade is cheap if I don't have to constantly correct fold, compression, other problems with dicey, frustrating bone sections. By the way, this has all been discussed before on Histonet, archives are searchable for more information. At 09:24 AM 1/15/2004 -0500, you wrote: >We have been using a warming plate to lay our sections on after we cut them. >This has helped them flatten out and stick to the plus slides better. >Loralee Gehan > >> ---------- >> From: Gayle Callis >> Sent: Wednesday, January 14, 2004 3:05 PM >> To: Mike Sohaskey; Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] folds forming in bone/cartilage sections >> >> Two things that may help >> >> One, be careful about overprocessing mouse bone, decalcified tibia need >> only 1 holur per change, with this schedule and hopefully you have vacuum >> and pressure. >> >> 70%, 80%, 95% X 2, 100% x 2, xylene X 1, Clearite 3 X 1, paraffin a >> minimum >> of 3 chnages (30, 30, 1 hour) Use harder paraffin to give more rigidity >> to >> support embedding media, Tissue Prep 2 is excellent or at least embed in >> this. Do not add heat to processing and make sure the paraffin is no >> hotter >> than 60C. If you remove too much of bound water from cartilage rather >> than >> free water in tissue spaces, you will have problems. What you are dealing >> with is softer growth plate cartilage surrounded by much harder bone. >> Soaking helps the harder decalcified bone soften while cartilage is >> picking >> up some water. Don't oversoak or bone will swell out of block = messy >> sections! >> >> Trim block, soak on ice block with water on top of ice for a few minutes, >> cut at 5 um using a really GOOD quality disposable blade, and lay ribbon >> on >> a waterbath containing approx 5% DMSO! Pick up section on plus charge >> slide, drain well and dry overnight at 37C. If your waterbath is too hot, >> wrinkles set and you cannot get them corrected. Adjust waterbath >> carefully. >> >> DMSO lowers the surface tension of water, and allows cartilage to stretch >> properly without having to pull on the paraffin ribbon. Your ribbon must >> be totally uncompressed and wrinkled to begin with and that requires the >> best blade. I have excellent luck with these knives, Dura Edge, AccueEdge >> and EdgeRite. We also prefer high profile for more stability, edges are >> as >> sharp as low profiles. 5 um sections are preferred, 10 um may give you >> more >> wrinkle problems. Low profile blades will work as long as the bones are >> perfectly processed. >> >> Good luck, >> >> >> >> At 10:36 AM 1/14/2004 -0800, you wrote: >> >Hello, >> > >> > I find that, when sectioning EDTA-decalcified mouse tibiae at >> >5 - 10 microns, that folds (i.e., creases) frequently form >> >specifically within the growth plate cartilage as the sections dry >> >and adhere to the Superfrost slides. I have had no success with >> >increasing the amount of time that I float the sections on a water >> >bath prior to drying them on slides. I'm hoping that someone might >> >be able to offer a suggestion as to how I can overcome this >> >difficulty. Thanks very much. >> > >> >MIKE >> > >> >Michael Sohaskey, Ph.D. >> >University of California, Berkeley >> >Molecular and Cell Biology >> >585 Life Sciences Addition >> >Berkeley, CA 94720-3200 >> >sohaskey@socrates.berkeley.edu >> >(510) 643-2775 (phone) >> > >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > >> > >> Gayle Callis >> MT,HT,HTL(ASCP) >> Research Histopathology Supervisor >> Veterinary Molecular Biology >> Montana State University - Bozeman >> PO Box 173610 >> Bozeman MT 59717-3610 >> 406 994-6367 (lab with voice mail) >> 406 994-4303 (FAX) >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Sue.Kapoor <@t> uhsi.org Thu Jan 15 10:03:49 2004 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] CDX-2 antibody source Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F552C@khmcexch.uhsi.org> Can anyone in Histoland direct me to a vendor for CDX-2 antibody? Thanks in advance, Sue Kapoor, HT(ASCP) Histology Supervisor Kenosha Medical Center Kenosha, WI From Linke_Noelle <@t> Allergan.com Thu Jan 15 10:42:33 2004 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] processor Message-ID: Hi all Does anyone have a good repairperson(s) who services Ventana processors in southern California (other than Ventana)? Thank you! Noelle Linke, BS, HTL(ASCP) Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From Rcartun <@t> harthosp.org Thu Jan 15 11:36:39 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] CDX-2 antibody source Message-ID: BioGenex. Richard Cartun >>> "Kapoor, Sue" 01/15/04 11:03AM >>> Can anyone in Histoland direct me to a vendor for CDX-2 antibody? Thanks in advance, Sue Kapoor, HT(ASCP) Histology Supervisor Kenosha Medical Center Kenosha, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Thu Jan 15 11:54:03 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Re: DMSO Message-ID: <494304423C63E246A5CF87A3AEEB577011B5CA@bumail01.barrynet.barry.edu> Although pure dimethylsulfoxide is only slightly toxic (less toxic than rubbing alcohol), DMSO carries anything dissolved in it through most gloves and through skin. If the DMSO contains anything you wouldn't want to eat, wear butyl rubber gloves when handling it. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, January 15, 2004 10:42 AM To: Leclerc Jocelyne; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: DMSO Whoops, broke a rule of mine, abbreviation DMSO is Dimethyl sulfoxide, and you can order it from any chemical supplier, it is a solvent commonly used in laboratories. Follow Material Safety Data Sheet instructions on handling this product. > Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Hi Gayle, >kann you tell me what you mean with DMSO and where I kann order this >product? Jocelyne Leclerc > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Susan.Walzer <@t> HCAHealthcare.com Thu Jan 15 08:40:28 2004 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Work load Message-ID: <49A0DB96A0A50C46A2D123454348B5E302138FD2@orlex07.orl.medcity.net> Until someone figures out how to cut blocks with automation the majority of our work in histology is still manual. Also we are doing more and more biopsies which require even more time. The more things change the more they stay the same..:) -----Original Message----- From: Waldner, Dale L MAJ MAMC [mailto:Dale.Waldner@nw.amedd.army.mil] Sent: Tuesday, January 13, 2004 4:29 PM To: 'eileen dusek'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Work load I am also very interested in any guidelines for evaluating productivity for histotechs. I have searched extensively for any standards but have come up empty-handed. I did find a reference to prior numbers being published based on the College of American Pathologists Laboratory Management Index Program (LMIP), but it is rather old and I believe, excluded automation in the laboratory. The LMIP seems like a great tool but would take some participation time prior to coming up with any meaningful data. Are there any standards available to give managers a general ballpark idea, for starters. The number of personnel Ms. Dusek has for her lab seems maybe even less than adequate to me, but who knows? Thank you. DW -----Original Message----- From: eileen dusek [mailto:eileen_dusek@yahoo.com] Sent: Tuesday, January 13, 2004 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Work load Hello everyone, A Pathologists was told by the "bean crunchers" they were overstaffed. What is your opinion? The lab does approximately 22,000 cases, Immunos on the Benchmark and a few Special Stains. The gross room is covered 95% of the time by Support Techs. This work load is performed by 2 FTE's and 1 Bench Supervisor. As we know the supervisor cannot bench all the time. Thanks Eileen Dusek --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Thu Jan 15 13:08:50 2004 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Contact # for CoPath Message-ID: <20040115.110931.5792.1360427@webmail23.nyc.untd.com> Hi Histonetters, Can someone give me a contact # or person for CoPath. Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From cormier <@t> MIT.EDU Thu Jan 15 14:07:57 2004 From: cormier <@t> MIT.EDU (Kathleen Cormier) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] paraffin to plastic embedding Message-ID: <5.0.2.1.2.20040115150338.00ac6d20@hesiod> Hello all, One of our researchers here has found some cool lesions in rabbit kidneys. Now he is asking for 1 micron sections. We of course have only the FFPE tissue. Is it possible to take the paraffin tissue and re-embedd it into plastic? What is recommended for future kidney lesions, type of plastic etc? Thanks!! Kathy Cormier Division of Comparative Medicine MIT From laurie.colbert <@t> huntingtonhospital.com Thu Jan 15 14:11:27 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Destaining slides Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BD67@EXCHANGE1.huntingtonhospital.com> Is there any way to de-stain either a retic or a trichrome and restain with H&E? Laurie Colbert Huntington Hospital Pasadena, CA From mprice26 <@t> juno.com Thu Jan 15 14:21:17 2004 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Salary and Wage Survey Message-ID: <20040115.122133.5792.1362118@webmail23.nyc.untd.com> Histonetters, Can someone direct me to the website for the latest wage and salary survey for Histology Techs. Also, I need to know what others are paying their OJT that are eligible to take the registry. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From siksik03 <@t> comcast.net Thu Jan 15 14:24:22 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Re: DMSO In-Reply-To: <494304423C63E246A5CF87A3AEEB577011B5CA@bumail01.barrynet.barry.edu> References: <494304423C63E246A5CF87A3AEEB577011B5CA@bumail01.barrynet.barry.edu> Message-ID: Dear HistoNetters I agree absolutely with this. I also want to point out that Paraplast Plus? paraffin has DMSO in it. If you read the container it comes in, it warns you not to get the wax on your hands! Of course, the DMSO is exactly what helps the paraffin penetrate the specimen better, but I would truly hesitate to use a wax which helped whatever else I was handling in the lab penetrate my skin, which is why I always counselled against its use in my microwaves. best regards, Steven Slap Microwave Consultant At 12:54 PM -0500 1/15/04, Smith, Allen wrote: >Although pure dimethylsulfoxide is only slightly toxic (less toxic than >rubbing alcohol), DMSO carries anything dissolved in it through most gloves >and through skin. If the DMSO contains anything you wouldn't want to eat, >wear butyl rubber gloves when handling it. From tflore <@t> lsuhsc.edu Thu Jan 15 15:23:45 2004 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] paraffin to plastic embedding Message-ID: Kathleen, yes, it is possible. You would first deparaffinize tissue : Xylene overnight agitate to remove all wax; Absolute ethanol; 95% ethanol; 75% ethanol; 50% ethanol; 25% ethanol then to preferred fixative, ours is Paraformaldehyde-G. Then process for HRLM-TEM. Our lab uses spur as epoxy of choice for diagnostic purposes as our stats are processed in 2 hrs, polymerized in 2 hrs and are HRLM sectioned the same day. That is the versitilaty of spur epoxy. But for research, epon or one of the other harder epoxies is recommended. Teresa -----Original Message----- From: Kathleen Cormier [mailto:cormier@MIT.EDU] Sent: Thursday, January 15, 2004 2:08 PM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin to plastic embedding Hello all, One of our researchers here has found some cool lesions in rabbit kidneys. Now he is asking for 1 micron sections. We of course have only the FFPE tissue. Is it possible to take the paraffin tissue and re-embedd it into plastic? What is recommended for future kidney lesions, type of plastic etc? Thanks!! Kathy Cormier Division of Comparative Medicine MIT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dave_laidley <@t> yahoo.ca Thu Jan 15 16:43:37 2004 From: dave_laidley <@t> yahoo.ca (David Laidley) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] glycol methacrylate and sectioning Message-ID: <20040115224337.78565.qmail@web20809.mail.yahoo.com> This may be a stupid question but can a sliding microtome cut brain tissue at 30 um when embedded in glycol methacrylate. I know that there exists rotory microtomes that cut glycol methacrylate but I can't seem to find any information about sliding microtomes. If you can cut 30 um sections of brain tissue in glycol methacrylate then does anyone have any tips on how this might be done (well). Or at least any tips or tricks you may have picked up over the years that may make the process go more smoothly. David Laidley (MSc student) Memorial University of Newfoundland Division of Basic Medical Sciences, Neuroscience dave_laidley@yahoo.ca --------------------------------- Post your free ad now! Yahoo! Canada Personals From gcallis <@t> montana.edu Thu Jan 15 17:14:14 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] paraffin to plastic embedding In-Reply-To: <5.0.2.1.2.20040115150338.00ac6d20@hesiod> Message-ID: <3.0.6.32.20040115161414.00bd38e0@gemini.msu.montana.edu> I have cut 1 um paraffin sections rather than have to take the paraffin out and put into GMA. If you don't remove the paraffin completely (several changes of xylene and then a couple of 100% ethanol) I would think any residual paraffin will cause polymerization problems. The tissue should be only 1 mm thick for good infiltration and polymerization with GMA. Glycol methacrylate will be excellent for 1 um but try paraffin first. I suggest you use an excellent disposable blade. Our 1 um paraffin sections were cut with AccuEdge, Sakura Finetek and either high or low profile will work. Make sure you block is cold, so you can get good firm support. IF you want to ever do immunostaining on plastic sections, then you should use polymethylmethacrylate since it can be totally removed (GMA, once polymerized, cannot be removed and is a poor embedding media for immunostaining). At 03:07 PM 1/15/2004 -0500, you wrote: >Hello all, > >One of our researchers here has found some cool lesions in rabbit kidneys. >Now he is asking for 1 micron sections. We of course have only the FFPE >tissue. Is it possible to take the paraffin tissue and re-embedd it into >plastic? What is recommended for future kidney lesions, type of plastic >etc? Thanks!! > >Kathy Cormier >Division of Comparative Medicine >MIT > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ploykasek <@t> phenopath.com Thu Jan 15 17:53:34 2004 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Cdx clone Message-ID: We are using clone Cdx2-88 from biogenex. We have had good performance with this antibody. Patti Loykasek Phenopath Laboratories Seattle, WA From M.Bromley <@t> dgri.scot.nhs.uk Fri Jan 16 03:17:17 2004 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] molar/normal solutions Message-ID: <7325D637DFE2D211928800902733A7F303B94A12@DGAMTBDCEMS> Dear All I enjoy these issues, they show we are helpful group with a sense of humour. However we should nowadays stick to molarity for our work and strive to adopt proper international units. Brits and Americans are probably the worst offenders with Fahrenheit, gallons and many other old fashioned units still used. Just in case you need to check your processing solutions: 100 proof alcohol is the concentration of alcohol that will ignite wet gun powder, But seriously don't try this Happy New Year and Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > From lpwenk <@t> covad.net Fri Jan 16 04:20:42 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Salary and Wage Survey References: <20040115.122133.5792.1362118@webmail23.nyc.untd.com> Message-ID: <003c01c3dc1a$805636a0$8732fea9@hppav> http://www.ascp.org/bor/center/center_research.asp Click on either part I or part II. A new version of Adobe is required, which can be loaded from this page, if needed. Some labs start their OJT as "trainees" at a lower wage - say 75% of a certified HT. Once they pass their registry, they go up to the regular HT pay scale. (Good incentive for people to take the registry exam, and good cost savings for the lab for hiring/paying people who are slower, can't do as much work in the beginning, and need more attention/help from other techs and supervisor.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Thursday, January 15, 2004 3:21 PM Subject: [Histonet] Salary and Wage Survey > > Histonetters, > Can someone direct me to the website for the latest wage and salary survey for Histology Techs. > > Also, I need to know what others are paying their OJT that are eligible to take the registry. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bamur <@t> alaska.net Thu Jan 15 19:04:06 2004 From: bamur <@t> alaska.net (Barbara Murray) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink Message-ID: <001901c3dbcc$a529d7a0$129f70d1@d3b1l9> Greetings, We have been using a solidifier for our formalin, putting it into biohazard boxes for pickup by the housekeeping dept. We were told by our Safety Officer that we can now pour it down the drain with lots of running water. For the ones of you who are using formalin, how are you disposing of it? Thanks for your replies. Have a great day and weekend! Barbara A. Murray,HT.(ASCP) The Alaska Native Medical Center Anchorage, Alaska From M.Bromley <@t> dgri.scot.nhs.uk Fri Jan 16 06:58:56 2004 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] molar/normal solutions Message-ID: <7325D637DFE2D211928800902733A7F303B94A16@DGAMTBDCEMS> No Jeff you can light gun powder wetted with 100 proof or above spirit (about 55% ethanol) In the days when the British Navy was all sailing ships this was the only test and was "proof" that the alcohol was at least 100 proof. This was very important when the rum ration was part of our man management scheme and was probably the reason the Royal Navy faired so well against all-comers. Mike -----Original Message----- From: Jeff Jurczak [SMTP:jjurczak@att.net] Sent: 16 January 2004 12:52 To: Mike Bromley Subject: Re: [Histonet] molar/normal solutions You just put gunpowder in 100 percent hooch and it ignites?! Really? Interesting.... ----- Original Message ----- From: "Mike Bromley" To: "Histonet (E-mail)" Sent: Friday, January 16, 2004 3:17 AM Subject: [Histonet] molar/normal solutions Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > From Tbarnhart <@t> primecare.org Fri Jan 16 07:40:21 2004 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] PAS with and without Diastase for glycogen Message-ID: <1779904B5E82D511914C00D0B793339205BFD7C5@exchangent> We have been having problems with this stain for sometime. It has worked hit and miss. The Schiff's reaction seems to be fine. Shouldn't most any liver control work? We have tried everything, new Schiff's, new controls, spitting, amylase...I'm outta ideas here. Could you please advise...any and all help would be appreciated. What am I missing??? Tammy Barnhart Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From jtsonger <@t> vt.edu Fri Jan 16 07:47:57 2004 From: jtsonger <@t> vt.edu (Jill Songer) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink In-Reply-To: <001901c3dbcc$a529d7a0$129f70d1@d3b1l9> References: <001901c3dbcc$a529d7a0$129f70d1@d3b1l9> Message-ID: <6.0.0.22.0.20040116084535.01ae13c8@pop.vt.edu> We collect ours in 5 gallon containers and our Environmental Health and Safety Dept. picks it up once a week. From there it gets picked up by a truck and hauled off to an unknown (at least on my part) destination. At 08:04 PM 1/15/2004, Barbara Murray wrote: >Greetings, >We have been using a solidifier for our formalin, putting it >into biohazard boxes for pickup by the housekeeping dept. We were told >by our Safety Officer that we can now pour it down the drain with lots of >running water. >For the ones of you who are using formalin, how are you disposing of it? >Thanks for your replies. Have a great day and weekend! > >Barbara A. Murray,HT.(ASCP) >The Alaska Native Medical Center >Anchorage, Alaska > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Jill Songer HT (ASCP) Histopathology Lab Veterinary Medical Teaching Hospital Virginia Tech From Jackie.O'Connor <@t> abbott.com Fri Jan 16 08:18:32 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink Message-ID: Your institution must comply with all local, state, and federal guidelines for disposal of hazardous waste. You can be held personally responsible for any infractions. Your safety guy may not have all the correct information. I don't know about Alaska (although I always imagined it as the last really pristine state) - but I've never worked in another state where formalin could be poured down the drain - EVER. In Hawaii, we joked about pouring it into a volcano, but really didn't want to ire Pele, the goddess of fire. At that time, (10 years ago) we were paying $1,500 per 55 gallon drum for disposal of xylene and formalin waste. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Jill Songer Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2004 07:47 AM To: Barbara Murray , Histonet@lists.utsouthwestern.edu cc: Subject: Re: [Histonet] Formalin down the sink We collect ours in 5 gallon containers and our Environmental Health and Safety Dept. picks it up once a week. From there it gets picked up by a truck and hauled off to an unknown (at least on my part) destination. At 08:04 PM 1/15/2004, Barbara Murray wrote: >Greetings, >We have been using a solidifier for our formalin, putting it >into biohazard boxes for pickup by the housekeeping dept. We were told >by our Safety Officer that we can now pour it down the drain with lots of >running water. >For the ones of you who are using formalin, how are you disposing of it? >Thanks for your replies. Have a great day and weekend! > >Barbara A. Murray,HT.(ASCP) >The Alaska Native Medical Center >Anchorage, Alaska > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Jill Songer HT (ASCP) Histopathology Lab Veterinary Medical Teaching Hospital Virginia Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FreidaC <@t> aol.com Fri Jan 16 08:20:40 2004 From: FreidaC <@t> aol.com (FreidaC@aol.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] PAS with and without Diastase for glycogen Message-ID: <1c6.141f472a.2d394d38@aol.com> Tammy: Many livers do not contain a lot of glycogen - especially autopsy livers, because few are well nourished and have a lot of stored glycogen at the time of death. A better control in my opinion is a section of cervix containing both endo- and ecto- cervix. The stratified squamous epithelium of the ectocervix contains glycogen which will disappear with diastase digestion, while the mucous glands in the endocervix do not contain glycogen, but rather mucin that will be positive with the PAS. This will not disappear with diastase digestion. This is also easier control tissue to find in most institutions. Freida Carson From Jackie.O'Connor <@t> abbott.com Fri Jan 16 08:40:51 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink Message-ID: I'm very sorry - I misunderstood. I interpreted it as pouring formalin down the sink without benefit of neutralizing. I guess I DO need another cup of coffee! Jackie Jill Cox 01/16/2004 08:34 AM To: Jackie.O'Connor@abbott.com cc: Subject: Re: [Histonet] Formalin down the sink I have been neutralizing it and pouring it down the drain. I was told by the state it was fine. The neutralux is an approved neutralizer for state and federal guidelines. Not sure about the spelling. Sorry. Sakura finetek makes it. Jackie.O'Connor@abbott.com wrote: Your institution must comply with all local, state, and federal guidelines for disposal of hazardous waste. You can be held personally responsible for any infractions. Your safety guy may not have all the correct information. I don't know about Alaska (although I always imagined it as the last really pristine state) - but I've never worked in another state where formalin could be poured down the drain - EVER. In Hawaii, we joked about pouring it into a volcano, but really didn't want to ire Pele, the goddess of fire. At that time, (10 years ago) we were paying $1,500 per 55 gallon drum for disposal of xylene and formalin waste. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Jill Songer Sent b! y: histonet-bounces@lists.utsouthwestern.edu 01/16/2004 07:47 AM To: Barbara Murray , Histonet@lists.utsouthwestern.edu cc: Subject: Re: [Histonet] Formalin down the sink We collect ours in 5 gallon containers and our Environmental Health and Safety Dept. picks it up once a week. From there it gets picked up by a truck and hauled off to an unknown (at least on my part) destination. At 08:04 PM 1/15/2004, Barbara Murray wrote: >Greetings, >We have been using a solidifier for our formalin, putting it >into biohazard boxes for pickup by the housekeeping dept. We were told >by our Safety Officer that we can now pour it down the drain with lots of >running water. >For the ones of you who are using formalin, how are you disposing of it? >Thanks for your replies. Have a great day and weekend! > >Barbara A. Murray,HT.(ASCP) >The Alaska Nat! ive Medical Center >Anchorage, Alaska > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Jill Songer HT (ASCP) Histopathology Lab Veterinary Medical Teaching Hospital Virginia Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From mcauliff <@t> umdnj.edu Fri Jan 16 08:49:59 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] PAS with and without Diastase for glycogen In-Reply-To: <1779904B5E82D511914C00D0B793339205BFD7C5@exchangent> References: <1779904B5E82D511914C00D0B793339205BFD7C5@exchangent> Message-ID: <4007FA17.2030507@umdnj.edu> Hi Tammy: So the diastase is the problem? Order a fresh bottle from Sigma. I have always had good results with their alpha amylase, 0.25% in 4mM sodium acetate buffer, pH 6.0 for 10-15 min at room temp. This is on liver (rat or mouse) fixed 48 hours in neutral buffered formalin. I found no need to warm the enzyme sol'n to 37 degrees. Geoff Barnhart, Tammy wrote: >We have been having problems with this stain for sometime. It has worked >hit and miss. The Schiff's reaction seems to be fine. Shouldn't most any >liver control work? We have tried everything, new Schiff's, new controls, >spitting, amylase...I'm outta ideas here. Could you please advise...any and >all help would be appreciated. What am I missing??? > >Tammy Barnhart > > > >Confidentiality Notice:This e-mail message is for sole use of intended >recipient(s) and may contain confidential and privileged information. Any >unauthorized review, use, disclosure, distribution, or copying is >prohibited. If you are not the intended recipient, please contact the sender >by replying to this e-mail and destroy/delete all copies of this e-mail >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Tbarnhart <@t> primecare.org Fri Jan 16 08:52:08 2004 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] PAS with and without Diastase for glycogen Message-ID: <1779904B5E82D511914C00D0B793339205BFD7C6@exchangent> Thank you all for your ideas, let me elaborate... Our pathologists won't except cervix for a control, they want a liver control. When we use cervix, the stain seems to work great. I have not tried skin or kidney, but maybe I can get them to except them as controls. We have tried spitting, it works best. I don't feel it is a problem with our procedure, because it seems to work well on cervix and the Schiff's reaction seems to be very strong. Freida, you may have hit the nail on the head with your comment on glycogen storage in the liver. Autopsy tissue is about all the liver we can get. Could be that we will never get good, consistent results with liver controls. However, in my previous lab we did get consistent staining with liver. That is what has me so perplexed. I think I will try the kidney. If alot of you respond that liver will never make a good control, maybe I can use that as proof to the pathologists that we need to use the cervix control. Any other ideas....please let me know. [Barnhart, Tammy] -----Original Message----- From: FreidaC@aol.com [mailto:FreidaC@aol.com] Sent: Friday, January 16, 2004 8:21 AM To: Tbarnhart@primecare.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS with and without Diastase for glycogen Tammy: Many livers do not contain a lot of glycogen - especially autopsy livers, because few are well nourished and have a lot of stored glycogen at the time of death. A better control in my opinion is a section of cervix containing both endo- and ecto- cervix. The stratified squamous epithelium of the ectocervix contains glycogen which will disappear with diastase digestion, while the mucous glands in the endocervix do not contain glycogen, but rather mucin that will be positive with the PAS. This will not disappear with diastase digestion. This is also easier control tissue to find in most institutions. Freida Carson Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From celebrej <@t> HHSC.CA Fri Jan 16 09:05:56 2004 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] PAS with and without Diastase for glycogen Message-ID: <3AADFB88753AD31189C100902786B91C0E41C0CD@hch_nt_exchange.hhsc.ca> Here's one idea..... Have you thought of trying a composite block made up of liver, cervix and even kidney? That way all bases are covered... > ---------- > From: Barnhart, Tammy[SMTP:Tbarnhart@primecare.org] > Sent: Friday, January 16, 2004 9:52 AM > To: 'FreidaC@aol.com'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] PAS with and without Diastase for glycogen > > Thank you all for your ideas, let me elaborate... > > Our pathologists won't except cervix for a control, they want a liver > control. When we use cervix, the stain seems to work great. I have not > tried skin or kidney, but maybe I can get them to except them as controls. > We have tried spitting, it works best. I don't feel it is a problem with > our procedure, because it seems to work well on cervix and the Schiff's > reaction seems to be very strong. Freida, you may have hit the nail on > the > head with your comment on glycogen storage in the liver. Autopsy tissue > is > about all the liver we can get. Could be that we will never get good, > consistent results with liver controls. However, in my previous lab we > did > get consistent staining with liver. That is what has me so perplexed. I > think I will try the kidney. If alot of you respond that liver will never > make a good control, maybe I can use that as proof to the pathologists > that > we need to use the cervix control. Any other ideas....please let me know. > > > [Barnhart, Tammy] -----Original Message----- > From: FreidaC@aol.com [mailto:FreidaC@aol.com] > Sent: Friday, January 16, 2004 8:21 AM > To: Tbarnhart@primecare.org; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS with and without Diastase for glycogen > > > > Tammy: > > Many livers do not contain a lot of glycogen - especially autopsy livers, > because few are well nourished and have a lot of stored glycogen at the > time > of death. A better control in my opinion is a section of cervix > containing > both endo- and ecto- cervix. The stratified squamous epithelium of the > ectocervix contains glycogen which will disappear with diastase digestion, > while the mucous glands in the endocervix do not contain glycogen, but > rather mucin that will be positive with the PAS. This will not disappear > with diastase digestion. This is also easier control tissue to find in > most > institutions. > > Freida Carson > > > > > Confidentiality Notice:This e-mail message is for sole use of intended > recipient(s) and may contain confidential and privileged information. Any > unauthorized review, use, disclosure, distribution, or copying is > prohibited. If you are not the intended recipient, please contact the > sender > by replying to this e-mail and destroy/delete all copies of this e-mail > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From mcauliff <@t> umdnj.edu Fri Jan 16 09:16:35 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] glycol methacrylate and sectioning In-Reply-To: <20040115224337.78565.qmail@web20809.mail.yahoo.com> References: <20040115224337.78565.qmail@web20809.mail.yahoo.com> Message-ID: <40080053.20205@umdnj.edu> Hi David: If you want 30 micron sections of brain why not frozen sections? or celloidin sections? I have never heard of cutting glycol methacrylate that thick, I always thought the purpose of plastic sections was thin sections. Perhaps the "plastic" people on this list can provide advice. Geoff David Laidley wrote: >This may be a stupid question but can a sliding microtome cut brain tissue at 30 um when embedded in glycol methacrylate. I know that there exists rotory microtomes that cut glycol methacrylate but I can't seem to find any information about sliding microtomes. > >If you can cut 30 um sections of brain tissue in glycol methacrylate then does anyone have any tips on how this might be done (well). Or at least any tips or tricks you may have picked up over the years that may make the process go more smoothly. > >David Laidley (MSc student) >Memorial University of Newfoundland >Division of Basic Medical Sciences, Neuroscience >dave_laidley@yahoo.ca > > > >--------------------------------- >Post your free ad now! Yahoo! Canada Personals >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Fri Jan 16 09:32:41 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Metric versus other units In-Reply-To: <7325D637DFE2D211928800902733A7F303B94A12@DGAMTBDCEMS> Message-ID: <3.0.6.32.20040116083241.00bd9f50@gemini.msu.montana.edu> Interesting, at home I use the units you describe, but in the lab I work in Metric system. Born in the USA! but with a sense of humor --- At 09:17 AM 1/16/2004 -0000, you wrote: >Dear All >I enjoy these issues, they show we are helpful group with a sense of humour. > >However we should nowadays stick to molarity for our work and strive to >adopt proper international units. Brits and Americans are probably the worst >offenders with Fahrenheit, gallons and many other old fashioned units still >used. > >Just in case you need to check your processing solutions: 100 proof alcohol >is the concentration of alcohol that will ignite wet gun powder, > >But seriously don't try this > >Happy New Year and >Best Wishes > >Mike Bromley > >Chief Biomedical Scientist >Pathology >Dumfries & Galloway Royal Infirmary >Scotland, UK > >> This e-mail and any files transmitted with it are private and intended >> solely for the use of the individual or entity to whom they are addressed. >> If you have received this e-mail in error please return it to the address >> it >> came from telling them it is not for you and then delete it from your >> system. >> >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From victor <@t> pathology.washington.edu Fri Jan 16 09:48:34 2004 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Friday Humor Message-ID: <400807D2.5020302@pathology.washington.edu> This is courtesy of my blonde wife. Last night I finally ported our cell numbers from AT&T to Verizon. After being home for awhile my wife asked how close were the new numbers to our old number. Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center 206-543-4823 From EliMarGo <@t> aol.com Fri Jan 16 09:51:56 2004 From: EliMarGo <@t> aol.com (EliMarGo@aol.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink Message-ID: <6F599AA4.1F0D99BE.0071FAF8@aol.com> We use to use a neutralizer. A few hours after the neutralizer has been poured in you can simply pour it down the drain. It was very good stuff. It eliminated the formal in stink and at the same time made it same for our environment. Elisa Gonzalez Research Tech.II Doheny Eye Institute From gcallis <@t> montana.edu Fri Jan 16 09:55:12 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Glycogen storage disease re:PAS with and without Diastase for glycogen In-Reply-To: <1779904B5E82D511914C00D0B793339205BFD7C6@exchangent> Message-ID: <3.0.6.32.20040116085512.00bd9f50@gemini.msu.montana.edu> Dear All, Isn't glycogen water soluble? You may need to use different fixative to retain glycogen in the first place, Hrapchak and Sheehan book has a list of fixatives recommended for different tissue components. Carnoys or alcoholic formalin, even pure alcohol fixation is recommended for glycogen storage disease with aqueous fixatives avoided. Hence NBF may NOT be the fixative of choice for what you want to see. We found this with fish liver project and if you have a research project going, a piece of liver in the proper fixative may solve some of your problems with other pieces collected in NBF for routine staining, etc. It very well may NOT be the staining method, but initial handling. This way the experiment is controlled with the special stain on THE target tissue (liver) containing glycogen. At 08:52 AM 1/16/2004 -0600, you wrote: >Thank you all for your ideas, let me elaborate... > >Our pathologists won't except cervix for a control, they want a liver >control. When we use cervix, the stain seems to work great. I have not >tried skin or kidney, but maybe I can get them to except them as controls. >We have tried spitting, it works best. I don't feel it is a problem with >our procedure, because it seems to work well on cervix and the Schiff's >reaction seems to be very strong. Freida, you may have hit the nail on the >head with your comment on glycogen storage in the liver. Autopsy tissue is >about all the liver we can get. Could be that we will never get good, >consistent results with liver controls. However, in my previous lab we did >get consistent staining with liver. That is what has me so perplexed. I >think I will try the kidney. If alot of you respond that liver will never >make a good control, maybe I can use that as proof to the pathologists that >we need to use the cervix control. Any other ideas....please let me know. > > >[Barnhart, Tammy] -----Original Message----- >From: FreidaC@aol.com [mailto:FreidaC@aol.com] >Sent: Friday, January 16, 2004 8:21 AM >To: Tbarnhart@primecare.org; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] PAS with and without Diastase for glycogen > > > >Tammy: > >Many livers do not contain a lot of glycogen - especially autopsy livers, >because few are well nourished and have a lot of stored glycogen at the time >of death. A better control in my opinion is a section of cervix containing >both endo- and ecto- cervix. The stratified squamous epithelium of the >ectocervix contains glycogen which will disappear with diastase digestion, >while the mucous glands in the endocervix do not contain glycogen, but >rather mucin that will be positive with the PAS. This will not disappear >with diastase digestion. This is also easier control tissue to find in most >institutions. > >Freida Carson > > > > >Confidentiality Notice:This e-mail message is for sole use of intended >recipient(s) and may contain confidential and privileged information. Any >unauthorized review, use, disclosure, distribution, or copying is >prohibited. If you are not the intended recipient, please contact the sender >by replying to this e-mail and destroy/delete all copies of this e-mail >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From EliMarGo <@t> aol.com Fri Jan 16 09:57:54 2004 From: EliMarGo <@t> aol.com (EliMarGo@aol.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink Message-ID: <4EFEB8DB.3E34E129.0071FAF8@aol.com> Sorry, I meant "formalin" and "safe". . .it really is too early in the morning. . . Elisa Gonzalez Research Tech.II Doheny Eye Institute From gcallis <@t> montana.edu Fri Jan 16 09:59:57 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] glycol methacrylate and sectioning In-Reply-To: <40080053.20205@umdnj.edu> References: <20040115224337.78565.qmail@web20809.mail.yahoo.com> <20040115224337.78565.qmail@web20809.mail.yahoo.com> Message-ID: <3.0.6.32.20040116085957.00bd9f50@gemini.msu.montana.edu> Having done glycol methacrylate, I agree with Geoff's comments. I have a feeling 30 um sections of GMA embedded tissues would shatter coming off the knife, and you would probably need an expensive tungsten carbide knife to even attempt this and a really powerful sliding microtome. We always reserved GMA methods for 3 um or less thickness, thicker we went back to paraffin, OR with bone, ground and polished sections in methylmethacrylate, but this would NOT be suitable for brain work, rather mineralized bone sections. Good luck. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) At 10:16 AM 1/16/2004 -0500, you wrote: >Hi David: > > If you want 30 micron sections of brain why not frozen sections? or >celloidin sections? I have never heard of cutting glycol methacrylate >that thick, I always thought the purpose of plastic sections was thin >sections. Perhaps the "plastic" people on this list can provide advice. > >Geoff > >David Laidley wrote: > >>This may be a stupid question but can a sliding microtome cut brain tissue at 30 um when embedded in glycol methacrylate. I know that there exists rotory microtomes that cut glycol methacrylate but I can't seem to find any information about sliding microtomes. >> >>If you can cut 30 um sections of brain tissue in glycol methacrylate then does anyone have any tips on how this might be done (well). Or at least any tips or tricks you may have picked up over the years that may make the process go more smoothly. >> >>David Laidley (MSc student) >>Memorial University of Newfoundland >>Division of Basic Medical Sciences, Neuroscience >>dave_laidley@yahoo.ca >> >> >> >>--------------------------------- >>Post your free ad now! Yahoo! Canada Personals >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > >-- >-- >********************************************** >Geoff McAuliffe, Ph.D. >Neuroscience and Cell Biology >Robert Wood Johnson Medical School >675 Hoes Lane, Piscataway, NJ 08854 >voice: (732)-235-4583; fax: -4029 >mcauliff@umdnj.edu >********************************************** > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From tpmorken <@t> labvision.com Fri Jan 16 10:35:48 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA203571F@usca0082k08.labvision.apogent.com> I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California From JWEEMS <@t> sjha.org Fri Jan 16 10:39:09 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798E0@exch4.sjha.org> But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will not blow down this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From JWEEMS <@t> sjha.org Fri Jan 16 10:40:08 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Friday Humor Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798E1@exch4.sjha.org> Don't you be ugly. Anyway, blondes have more fun... Have a great weekend everyone! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victor Tobias Sent: Friday, January 16, 2004 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Humor This is courtesy of my blonde wife. Last night I finally ported our cell numbers from AT&T to Verizon. After being home for awhile my wife asked how close were the new numbers to our old number. Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center 206-543-4823 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From mprice26 <@t> juno.com Fri Jan 16 10:43:08 2004 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Ht Certification Route 3 Message-ID: <20040116.084317.5566.1031929@webmail18.nyc.untd.com> Histonetters, I know this has been discussed several times. And I did save this info but on my home computer. The question: I have an employee that her 2 year OJT will not be complete until December of this year. So, she will not be able to become an registered HT through Route 3 Is this correct? Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From BRobert <@t> ameripath.com Fri Jan 16 10:48:21 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Formalin down the sink Message-ID: Barbara, You know like me how hazardous formalin is, the best, safest and most economical way to dispose of it based on my experience is to neutralise it first and then dispose of it down the drain after testing the completed reaction. We use Formalex from American Bio Safety cat# FX-05. We neutralize at the end of the day, let sit overnight and test and dump the following morning. It works really well. Good luck! Brigitte Robert Histology Lab. Manager A.P.M.G Inc. Los Gatos, CA -----Original Message----- From: Barbara Murray [mailto:bamur@alaska.net] Sent: Thursday, January 15, 2004 5:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the sink Greetings, We have been using a solidifier for our formalin, putting it into biohazard boxes for pickup by the housekeeping dept. We were told by our Safety Officer that we can now pour it down the drain with lots of running water. For the ones of you who are using formalin, how are you disposing of it? Thanks for your replies. Have a great day and weekend! Barbara A. Murray,HT.(ASCP) The Alaska Native Medical Center Anchorage, Alaska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chris.goodall <@t> bristol.ac.uk Fri Jan 16 11:08:47 2004 From: chris.goodall <@t> bristol.ac.uk (anclg) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Staining nerve fibres in human palate Message-ID: <000701c3dc53$67c66f40$8f6ede89@hist143> Does anyone have any suggestions as to the most reliable way of staining nerve fibres in a complete human palate? I was wondering about sudan black on frozen sections, or one of the silver stains for paraffin sections.I have been asked to do this in order to reconstruct a 3D image of the nerves. Thanks. Chris Goodall From Jackie.O'Connor <@t> abbott.com Fri Jan 16 10:58:31 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Chicago is a balmy 25, with freezing rain and sleet predicted for this evening. Perfect. Why do we live here? Does anyone have a scientific reason for temps to get to -100F (windchill is what I heard for the East). Don't bugs die when it's warmer? Don't some just stay frozen and pop back up again in the spring? I don't see any reason for it to be this cold!!! (I don't see a scientific reason to support the existence of mosquitoes, either) Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2004 10:39 AM To: "'Morken, Tim - Labvision'" , Histonet@lists.utsouthwestern.edu cc: Subject: RE: [Histonet] Hope you all aren't freezing in the east! But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will not blow down this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Jan 16 11:06:38 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Ht Certification Route 3 Message-ID: Marsha, It would seem to me that if your employee's application to take the exam is turned in before Jan1, she will be eligible however I would urge you to call the BOR to confirm this information. If for example she becomes eligible on Dec 31, I would hope that a faxed application may be acceptable however you may learn that the BOR is allowing a grace period to prevent individuals in this situation from being excluded. please let us know what you learn Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> 01/16/04 11:43AM >>> Histonetters, I know this has been discussed several times. And I did save this info but on my home computer. The question: I have an employee that her 2 year OJT will not be complete until December of this year. So, she will not be able to become an registered HT through Route 3 Is this correct? Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From OBrennan1 <@t> rcsi.ie Fri Jan 16 11:12:27 2004 From: OBrennan1 <@t> rcsi.ie (Orlaith Brennan) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] (no subject) Message-ID: <3E9C9E677B39D249BA3B0A0AB81ED94D08ABAB@excha_stgsan1.rcsi-internal.ie> > I'm working on apoptosis in ovine osteocytes. We plan on doing TUNEL staining and also Caspase-3 antibody staining. I was wondering how long from the point the animal dies do you have to freeze the samples before the DNA and proteins will start to deteriorate? Is freezing necessarily the best method of preserving the samples, or should we consider an alternative? Has anyone performed either tunel or caspase 3 staining on undecalcified bone sections? > > We also plan on looking at other proteins within the bone, inparticular nitric oxide synthase. Again, how long before this protein begins to denature? Does anyone have a protocol for the preservation of bone proteins? > > Is there a commercial storage solution for proteins (similar to ambions RNALater)? If so would this be effective for a couple of hours to allow the samples be transported? > > Has anyone used a large bone biopsy set? > > Any advice? > > Thank You, > > Orlaith Brennan > > > > -------------------------------------------------------------------------------------------------------------------- This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of The Royal College Of Surgeons in Ireland. -------------------------------------------------------------------------------------------------------------------- From tbergeron <@t> criver.com Fri Jan 16 11:12:37 2004 From: tbergeron <@t> criver.com (tbergeron@criver.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Those of us here in the North East would GLADLY even take any warmer weather that you can send this way... Last night they said we were going to get -30 to -60 wind chills over night.. This am, not sure what the wind chill was but along with it being -10, it wasn't too pleasant a day that's for sure.. Tracy E. Bergeron, HT(ASCP) Histology Laboratory Charles River Laboratories Wilmington, MA 978-658-6000 x-1229 |---------+-----------------------------------------> | | Jackie.O'Connor@abbott.com | | | Sent by: | | | histonet-bounces@lists.utsouth| | | western.edu | | | | | | | | | 01/16/2004 11:58 AM | | | | |---------+-----------------------------------------> >---------------------------------------------------------------------------------------------------------------| | | | To: "Weems, Joyce" | | cc: Histonet@lists.utsouthwestern.edu | | Subject: RE: [Histonet] Hope you all aren't freezing in the east! | >---------------------------------------------------------------------------------------------------------------| Chicago is a balmy 25, with freezing rain and sleet predicted for this evening. Perfect. Why do we live here? Does anyone have a scientific reason for temps to get to -100F (windchill is what I heard for the East). Don't bugs die when it's warmer? Don't some just stay frozen and pop back up again in the spring? I don't see any reason for it to be this cold!!! (I don't see a scientific reason to support the existence of mosquitoes, either) Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2004 10:39 AM To: "'Morken, Tim - Labvision'" , Histonet@lists.utsouthwestern.edu cc: Subject: RE: [Histonet] Hope you all aren't freezing in the east! But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will not blow down this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Fri Jan 16 11:17:32 2004 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Please unsubscribe Message-ID: <61135F0455D33347B5AAE209B903A30403262346@EXCHVS2.medctr.ad.wfubmc.edu> I will be out of the office from 1/16 to 1/29. Martha Ward From lefortb <@t> crhsc.umontreal.ca Fri Jan 16 10:18:07 2004 From: lefortb <@t> crhsc.umontreal.ca (Bertrand Lefort) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Detection level In-Reply-To: Message-ID: Hello, I am used to do immuno-cyto-fluorecence on different receptors and peptides. I would like to know if there is someone who has used different technology to find the detection level of this technique. I imagine that I can see a signal if there is a certain number of molecules (10000 - 100000 receptor per cells ???). Have someone an idea of this number ? Bertrand From Linke_Noelle <@t> Allergan.com Fri Jan 16 11:23:56 2004 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Tim is right. It's kind of cloudy here in southern California too, and it's only supposed to get up to 70 today. Very sad. Noelle (damn glad she left Boston) Linke, BS, HTL(ASCP) Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Friday, January 16, 2004 8:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Jan 16 11:30:57 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Hmmmm....Here in Atlanta it's chilly in the mornings (around 32) and lovely in the afternoon (around 55). Not as warm as California but fewer fires, earthquakes, floods and mudslides! Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim - Labvision Sent: Fri 1/16/2004 11:35 AM To: Histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Jan 16 11:44:51 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] (no subject) In-Reply-To: <3E9C9E677B39D249BA3B0A0AB81ED94D08ABAB@excha_stgsan1.rcsi- internal.ie> Message-ID: <3.0.6.32.20040116104451.00bcfbc8@gemini.msu.montana.edu> I suggest you access RA Dodds publications on doing undecalcified bone frozen sections. By the way, what is a "large bone biopsy set"? A trephine? At 05:12 PM 1/16/2004 -0000, you wrote: > > > >> I'm working on apoptosis in ovine osteocytes. We plan on doing TUNEL staining and also Caspase-3 antibody staining. I was wondering how long from the point the animal dies do you have to freeze the samples before the DNA and proteins will start to deteriorate? Is freezing necessarily the best method of preserving the samples, or should we consider an alternative? Has anyone performed either tunel or caspase 3 staining on undecalcified bone sections? >> >> We also plan on looking at other proteins within the bone, inparticular nitric oxide synthase. Again, how long before this protein begins to denature? Does anyone have a protocol for the preservation of bone proteins? >> >> Is there a commercial storage solution for proteins (similar to ambions RNALater)? If so would this be effective for a couple of hours to allow the samples be transported? >> >> Has anyone used a large bone biopsy set? >> >> Any advice? >> >> Thank You, >> >> Orlaith Brennan >> >> >> >> >--------------------------------------------------------------------------- ----------------------------------------- >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom >they are addressed. >If you have received this email in error please notify the >originator of the message. This footer also confirms that this >email message has been scanned for the presence of computer viruses. > >Any views expressed in this message are those of the individual >sender, except where the sender specifies and with authority, >states them to be the views of The Royal College Of Surgeons in Ireland. > >--------------------------------------------------------------------------- ----------------------------------------- >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ldunikoski <@t> rbmc.org Fri Jan 16 11:52:09 2004 From: ldunikoski <@t> rbmc.org (Dunikoski, Leonard PhD) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <7A2DF880A7A1D511A63300508BF7D5550271AD00@es1.rbmc.org> Just remember the words of Scripture: Many are cold, but few are frozen Leonard K. Dunikoski, Ph.D. Director of Operations Raritan Bay Medical Center Perth Amboy, NJ 08861 -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: The information included in this email contains confidential information belonging to the sender. This information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents hereof is strictly prohibited. If you have received this email in error, please notify the sender immediately. From gcallis <@t> montana.edu Fri Jan 16 12:00:00 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Whoops, forgot to put undecalcified bone frozens Message-ID: <3.0.6.32.20040116110000.00bd1818@gemini.msu.montana.edu> I suggest you access RA Dodds publications on doing undecalcified bone frozen sections - a difficult task without Instrumedics Cryojane. By the way, what is a "large bone biopsy set"? A trephine? Wintering in Montana is sometimes with -75F chill factor with wind blowing and sunshine. An Arctic Express aka Clipper make us hearty and tough in this Wild, Wild West so we just wear down filled everything! Can almost do frozen sections outside with a razor blade! Have great sympathy for those who are not prepared for super-cold. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From jfish <@t> gladstone.ucsf.edu Fri Jan 16 12:02:30 2004 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! In-Reply-To: <0556BE8AC5551E4E8AF6BB9E42509BA203571F@usca0082k08.labvision.apogent.com> References: <0556BE8AC5551E4E8AF6BB9E42509BA203571F@usca0082k08.labvision.apogent.com> Message-ID: Tim, Yeah, and the sun only peeped through the clouds for a just few minutes yesterday here in San Francisco! I told someone working here how cold it was back there, and she said "I didn't know temperatures that low were possible!". >Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 From Lynne.Bell <@t> hitchcock.org Fri Jan 16 12:02:16 2004 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Here in central Vermont it was a balmy minus 16 this morning with about 30 mph winds. Much warmer than yesterday morning however, which was minus 27 with 30 mph winds. My husband is threatening to take the dog and move to Florida!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From peptolab <@t> hamptons.com Fri Jan 16 12:10:24 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Re: Histonet Digest, Vol 2, Issue 19 References: Message-ID: <001b01c3dc5c$044f1760$95a5bd18@JEFF> This weather sucks. I slid sideways into a telephone pole at 25 MPH and bent my right front wheel inwards trying to get to the lab at 4:45 AM yesterday. Five day weekend for me. Jeff Silverman ----- Original Message ----- From: To: Sent: Friday, January 16, 2004 1:00 PM Subject: Histonet Digest, Vol 2, Issue 19 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: glycol methacrylate and sectioning (Gayle Callis) > 2. Hope you all aren't freezing in the east! > (Morken, Tim - Labvision) > 3. RE: Hope you all aren't freezing in the east! (Weems, Joyce) > 4. RE: Friday Humor (Weems, Joyce) > 5. Ht Certification Route 3 (mprice26@juno.com) > 6. RE: Formalin down the sink (BRobert@ameripath.com) > 7. Staining nerve fibres in human palate (anclg) > 8. RE: Hope you all aren't freezing in the east! > (Jackie.O'Connor@abbott.com) > 9. Re: Ht Certification Route 3 (Vinnie Della Speranza) > 10. (no subject) (Orlaith Brennan) > 11. RE: Hope you all aren't freezing in the east! > (tbergeron@criver.com) > 12. Please unsubscribe (Martha Ward) > 13. Detection level (Bertrand Lefort) > 14. RE: Hope you all aren't freezing in the east! (Linke_Noelle) > 15. RE: Hope you all aren't freezing in the east! (Bartlett, Jeanine) > 16. Re: (no subject) (Gayle Callis) > 17. RE: Hope you all aren't freezing in the east! > (Dunikoski, Leonard PhD) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 Jan 2004 08:59:57 -0700 > From: Gayle Callis > Subject: Re: [Histonet] glycol methacrylate and sectioning > To: Geoff McAuliffe , > Histonet@lists.utsouthwestern.edu > Message-ID: <3.0.6.32.20040116085957.00bd9f50@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii" > > Having done glycol methacrylate, I agree with Geoff's comments. I have a > feeling 30 um sections of GMA embedded tissues would shatter coming off the > knife, and you would probably need an expensive tungsten carbide knife to > even attempt this and a really powerful sliding microtome. We always > reserved GMA methods for 3 um or less thickness, thicker we went back to > paraffin, OR with bone, ground and polished sections in methylmethacrylate, > but this would NOT be suitable for brain work, rather mineralized bone > sections. > > Good luck. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > At 10:16 AM 1/16/2004 -0500, you wrote: > >Hi David: > > > > If you want 30 micron sections of brain why not frozen sections? or > >celloidin sections? I have never heard of cutting glycol methacrylate > >that thick, I always thought the purpose of plastic sections was thin > >sections. Perhaps the "plastic" people on this list can provide advice. > > > >Geoff > > > >David Laidley wrote: > > > >>This may be a stupid question but can a sliding microtome cut brain > tissue at 30 um when embedded in glycol methacrylate. I know that there > exists rotory microtomes that cut glycol methacrylate but I can't seem to > find any information about sliding microtomes. > >> > >>If you can cut 30 um sections of brain tissue in glycol methacrylate then > does anyone have any tips on how this might be done (well). Or at least any > tips or tricks you may have picked up over the years that may make the > process go more smoothly. > >> > >>David Laidley (MSc student) > >>Memorial University of Newfoundland > >>Division of Basic Medical Sciences, Neuroscience > >>dave_laidley@yahoo.ca > >> > >> > >> > >>--------------------------------- > >>Post your free ad now! Yahoo! Canada Personals > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > > > >-- > >-- > >********************************************** > >Geoff McAuliffe, Ph.D. > >Neuroscience and Cell Biology > >Robert Wood Johnson Medical School > >675 Hoes Lane, Piscataway, NJ 08854 > >voice: (732)-235-4583; fax: -4029 > >mcauliff@umdnj.edu > >********************************************** > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 16 Jan 2004 08:35:48 -0800 > From: "Morken, Tim - Labvision" > Subject: [Histonet] Hope you all aren't freezing in the east! > To: Histonet@lists.utsouthwestern.edu > Message-ID: > <0556BE8AC5551E4E8AF6BB9E42509BA203571F@usca0082k08.labvision.apogent.com> > > Content-Type: text/plain > > I just want all of our collegues on the east coast (of US) to know we on the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > > > ------------------------------ > > Message: 3 > Date: Fri, 16 Jan 2004 11:39:09 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > To: "'Morken, Tim - Labvision'" , > Histonet@lists.utsouthwestern.edu > Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798E0@exch4.sjha.org> > Content-Type: text/plain; charset="utf-8" > > But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will > not > blow down this way! > > j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, > Tim - Labvision > Sent: Friday, January 16, 2004 11:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hope you all aren't freezing in the east! > > > I just want all of our collegues on the east coast (of US) to know we on > the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy > today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph??Ts Health System, Inc. > > > ------------------------------ > > Message: 4 > Date: Fri, 16 Jan 2004 11:40:08 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Friday Humor > To: 'Victor Tobias' , > Histonet@lists.utsouthwestern.edu > Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798E1@exch4.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Don't you be ugly. Anyway, blondes have more fun... > > Have a great weekend everyone! j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victor > Tobias > Sent: Friday, January 16, 2004 10:49 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Friday Humor > > > This is courtesy of my blonde wife. Last night I finally ported our cell > > numbers from AT&T to Verizon. After being home for awhile my wife asked > how close were the new numbers to our old number. > > Victor > > -- > Victor Tobias > Clinical Applications Analyst > Dept of Pathology > University of Washington Medical Center > 206-543-4823 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph??Ts Health System, Inc. > > > ------------------------------ > > Message: 5 > Date: Fri, 16 Jan 2004 16:43:08 GMT > From: mprice26@juno.com > Subject: [Histonet] Ht Certification Route 3 > To: histonet@lists.utsouthwestern.edu > Message-ID: <20040116.084317.5566.1031929@webmail18.nyc.untd.com> > Content-Type: text/plain > > > Histonetters, > I know this has been discussed several times. And I did save this info but on my home computer. > > The question: > I have an employee that her 2 year OJT will not be complete until December of this year. So, she will not be able to become an registered HT through Route 3 Is this correct? > > Thank you. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > > > ------------------------------ > > Message: 6 > Date: Fri, 16 Jan 2004 11:48:21 -0500 > From: BRobert@ameripath.com > Subject: RE: [Histonet] Formalin down the sink > To: bamur@alaska.net, Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Barbara, > You know like me how hazardous formalin is, the best, safest and most > economical way to dispose of it based on my experience is to neutralise it > first and then dispose of it down the drain after testing the completed > reaction. > We use Formalex from American Bio Safety cat# FX-05. We neutralize at the > end of the day, let sit overnight and test and dump the following morning. > It works really well. > Good luck! > > Brigitte Robert > Histology Lab. Manager > A.P.M.G Inc. Los Gatos, CA > > -----Original Message----- > From: Barbara Murray [mailto:bamur@alaska.net] > Sent: Thursday, January 15, 2004 5:04 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin down the sink > > > Greetings, > We have been using a solidifier for our formalin, putting it into biohazard > boxes for pickup by the housekeeping dept. We were told by our Safety > Officer that we can now pour it down the drain with lots of running water. > For the ones of you who are using formalin, how are you disposing of it? > Thanks for your replies. Have a great day and weekend! > > Barbara A. Murray,HT.(ASCP) > The Alaska Native Medical Center > Anchorage, Alaska > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 16 Jan 2004 17:08:47 -0000 > From: anclg > Subject: [Histonet] Staining nerve fibres in human palate > To: histonet > Message-ID: <000701c3dc53$67c66f40$8f6ede89@hist143> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have any suggestions as to the most reliable way of > staining nerve fibres in a complete human palate? I was wondering about > sudan black on frozen sections, or one of the silver stains for paraffin > sections.I have been asked to do this in order to reconstruct a 3D image > of the nerves. Thanks. > Chris Goodall > > > > > ------------------------------ > > Message: 8 > Date: Fri, 16 Jan 2004 10:58:31 -0600 > From: Jackie.O'Connor@abbott.com > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > To: "Weems, Joyce" > Cc: Histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > Chicago is a balmy 25, with freezing rain and sleet predicted for this > evening. Perfect. Why do we live here? > > Does anyone have a scientific reason for temps to get to -100F (windchill > is what I heard for the East). Don't bugs die when it's warmer? > Don't some just stay frozen and pop back up again in the spring? I don't > see any reason for it to be this cold!!! (I don't see a scientific reason > to support the existence of mosquitoes, either) > > Jacqueline M. O'Connor HT(ASCP) > Abbott Laboratories > Global Pharmaceutical Research and Development > Discovery Chemotheraputics > 847.938.4919 > > > > > > "Weems, Joyce" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/16/2004 10:39 AM > > > To: "'Morken, Tim - Labvision'" , > Histonet@lists.utsouthwestern.edu > cc: > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > > > But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will > not > blow down this way! > > j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, > Tim - Labvision > Sent: Friday, January 16, 2004 11:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hope you all aren't freezing in the east! > > > I just want all of our collegues on the east coast (of US) to know we on > the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy > today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Fri, 16 Jan 2004 12:06:38 -0500 > From: "Vinnie Della Speranza" > Subject: Re: [Histonet] Ht Certification Route 3 > To: , > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Marsha, > It would seem to me that if your employee's application to take the exam is turned in before Jan1, she will be eligible however I would urge you to call the BOR to confirm this information. If for example she becomes eligible on Dec 31, I would hope that a faxed application may be acceptable however you may learn that the BOR is allowing a grace period to prevent individuals in this situation from being excluded. please let us know what you learn > > Vinnie > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, SC 29425 > Ph: 843-792-6353 > fax: 843-792-8974 > > >>> 01/16/04 11:43AM >>> > > Histonetters, > I know this has been discussed several times. And I did save this info but on my home computer. > > The question: > I have an employee that her 2 year OJT will not be complete until December of this year. So, she will not be able to become an registered HT through Route 3 Is this correct? > > Thank you. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Fri, 16 Jan 2004 17:12:27 -0000 > From: "Orlaith Brennan" > Subject: [Histonet] (no subject) > To: > Message-ID: > <3E9C9E677B39D249BA3B0A0AB81ED94D08ABAB@excha_stgsan1.rcsi-internal.ie> > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > I'm working on apoptosis in ovine osteocytes. We plan on doing TUNEL staining and also Caspase-3 antibody staining. I was wondering how long from the point the animal dies do you have to freeze the samples before the DNA and proteins will start to deteriorate? Is freezing necessarily the best method of preserving the samples, or should we consider an alternative? Has anyone performed either tunel or caspase 3 staining on undecalcified bone sections? > > > > We also plan on looking at other proteins within the bone, inparticular nitric oxide synthase. Again, how long before this protein begins to denature? Does anyone have a protocol for the preservation of bone proteins? > > > > Is there a commercial storage solution for proteins (similar to ambions RNALater)? If so would this be effective for a couple of hours to allow the samples be transported? > > > > Has anyone used a large bone biopsy set? > > > > Any advice? > > > > Thank You, > > > > Orlaith Brennan > > > > > > > > > -------------------------------------------------------------------------- ------------------------------------------ > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of The Royal College Of Surgeons in Ireland. > > -------------------------------------------------------------------------- ------------------------------------------ > > ------------------------------ > > Message: 11 > Date: Fri, 16 Jan 2004 12:12:37 -0500 > From: tbergeron@criver.com > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > To: Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > > > Those of us here in the North East would GLADLY even take any warmer > weather that you can send this way... > > Last night they said we were going to get -30 to -60 wind chills over > night.. This am, not sure what the wind chill was but along with it being > -10, it wasn't too pleasant a day that's for sure.. > > > Tracy E. Bergeron, HT(ASCP) > Histology Laboratory > Charles River Laboratories > Wilmington, MA > 978-658-6000 > x-1229 > > > |---------+-----------------------------------------> > | | Jackie.O'Connor@abbott.com | > | | Sent by: | > | | histonet-bounces@lists.utsouth| > | | western.edu | > | | | > | | | > | | 01/16/2004 11:58 AM | > | | | > |---------+-----------------------------------------> > >--------------------------------------------------------------------------- ------------------------------------| > | | > | To: "Weems, Joyce" | > | cc: Histonet@lists.utsouthwestern.edu | > | Subject: RE: [Histonet] Hope you all aren't freezing in the east! | > >--------------------------------------------------------------------------- ------------------------------------| > > > > > Chicago is a balmy 25, with freezing rain and sleet predicted for this > evening. Perfect. Why do we live here? > > Does anyone have a scientific reason for temps to get to -100F (windchill > is what I heard for the East). Don't bugs die when it's warmer? > Don't some just stay frozen and pop back up again in the spring? I don't > see any reason for it to be this cold!!! (I don't see a scientific reason > to support the existence of mosquitoes, either) > > Jacqueline M. O'Connor HT(ASCP) > Abbott Laboratories > Global Pharmaceutical Research and Development > Discovery Chemotheraputics > 847.938.4919 > > > > > > "Weems, Joyce" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/16/2004 10:39 AM > > > To: "'Morken, Tim - Labvision'" , > Histonet@lists.utsouthwestern.edu > cc: > Subject: RE: [Histonet] Hope you all aren't freezing in the > east! > > > But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will > not > blow down this way! > > j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, > Tim - Labvision > Sent: Friday, January 16, 2004 11:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hope you all aren't freezing in the east! > > > I just want all of our collegues on the east coast (of US) to know we on > the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy > today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph's Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > ------------------------------ > > Message: 12 > Date: Fri, 16 Jan 2004 12:17:32 -0500 > From: "Martha Ward" > Subject: [Histonet] Please unsubscribe > To: > Message-ID: > <61135F0455D33347B5AAE209B903A30403262346@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="US-ASCII" > > I will be out of the office from 1/16 to 1/29. > Martha Ward > > > ------------------------------ > > Message: 13 > Date: Fri, 16 Jan 2004 12:18:07 -0400 > From: "Bertrand Lefort" > Subject: [Histonet] Detection level > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hello, > > I am used to do immuno-cyto-fluorecence on different receptors and peptides. > > I would like to know if there is someone who has used different technology > to find the detection level of this technique. > > I imagine that I can see a signal if there is a certain number of molecules > (10000 - 100000 receptor per cells ???). > > Have someone an idea of this number ? > > Bertrand > > > > > ------------------------------ > > Message: 14 > Date: Fri, 16 Jan 2004 09:23:56 -0800 > From: "Linke_Noelle" > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > To: "Morken, Tim - Labvision" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Tim is right. It's kind of cloudy here in southern California too, and > it's only supposed to get up to 70 today. Very sad. > > Noelle (damn glad she left Boston) Linke, BS, HTL(ASCP) > Allergan, Inc > 2525 Dupont Drive RD-2A > Irvine, CA 92612 > 714-246-5568 > > > -----Original Message----- > From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] > Sent: Friday, January 16, 2004 8:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hope you all aren't freezing in the east! > > I just want all of our collegues on the east coast (of US) to know we on > the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy > today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 15 > Date: Fri, 16 Jan 2004 12:30:57 -0500 > From: "Bartlett, Jeanine" > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > To: "Morken, Tim - Labvision" , > > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Hmmmm....Here in Atlanta it's chilly in the mornings (around 32) and lovely in the afternoon (around 55). Not as warm as California but fewer fires, earthquakes, floods and mudslides! > > Jeanine Bartlett > CDC/Atlanta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim - Labvision > Sent: Fri 1/16/2004 11:35 AM > To: Histonet@lists.utsouthwestern.edu > Cc: > Subject: [Histonet] Hope you all aren't freezing in the east! > > > > I just want all of our collegues on the east coast (of US) to know we on the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Fri, 16 Jan 2004 10:44:51 -0700 > From: Gayle Callis > Subject: Re: [Histonet] (no subject) > To: "Orlaith Brennan" , > Histonet@lists.utsouthwestern.edu > Message-ID: <3.0.6.32.20040116104451.00bcfbc8@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii" > > I suggest you access RA Dodds publications on doing undecalcified bone > frozen sections. > > By the way, what is a "large bone biopsy set"? A trephine? > > At 05:12 PM 1/16/2004 -0000, you wrote: > > > > > > > >> I'm working on apoptosis in ovine osteocytes. We plan on doing TUNEL > staining and also Caspase-3 antibody staining. I was wondering how long > from the point the animal dies do you have to freeze the samples before the > DNA and proteins will start to deteriorate? Is freezing necessarily the > best method of preserving the samples, or should we consider an > alternative? Has anyone performed either tunel or caspase 3 staining on > undecalcified bone sections? > >> > >> We also plan on looking at other proteins within the bone, inparticular > nitric oxide synthase. Again, how long before this protein begins to > denature? Does anyone have a protocol for the preservation of bone proteins? > >> > >> Is there a commercial storage solution for proteins (similar to ambions > RNALater)? If so would this be effective for a couple of hours to allow the > samples be transported? > >> > >> Has anyone used a large bone biopsy set? > >> > >> Any advice? > >> > >> Thank You, > >> > >> Orlaith Brennan > >> > >> > >> > >> > >--------------------------------------------------------------------------- > ----------------------------------------- > >This email and any files transmitted with it are confidential and > >intended solely for the use of the individual or entity to whom > >they are addressed. > >If you have received this email in error please notify the > >originator of the message. This footer also confirms that this > >email message has been scanned for the presence of computer viruses. > > > >Any views expressed in this message are those of the individual > >sender, except where the sender specifies and with authority, > >states them to be the views of The Royal College Of Surgeons in Ireland. > > > >--------------------------------------------------------------------------- > ----------------------------------------- > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > > > ------------------------------ > > Message: 17 > Date: Fri, 16 Jan 2004 12:52:09 -0500 > From: "Dunikoski, Leonard PhD" > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > To: Histonet@lists.utsouthwestern.edu > Message-ID: <7A2DF880A7A1D511A63300508BF7D5550271AD00@es1.rbmc.org> > Content-Type: text/plain > > Just remember the words of Scripture: > > Many are cold, but few are frozen > > > Leonard K. Dunikoski, Ph.D. > Director of Operations > Raritan Bay Medical Center > Perth Amboy, NJ 08861 > > > -----Original Message----- > From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] > Sent: Friday, January 16, 2004 11:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hope you all aren't freezing in the east! > > I just want all of our collegues on the east coast (of US) to know we on the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > NOTICE: The information included in this email contains confidential > information belonging to the sender. This information is intended only for > the use of the individual or entity named above. If you are not the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or action taken in reliance on the contents hereof is strictly > prohibited. If you have received this email in error, please notify the > sender immediately. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 2, Issue 19 > *************************************** From nena.dimaano <@t> stryker.com Fri Jan 16 12:06:58 2004 From: nena.dimaano <@t> stryker.com (Dimaano, Nena) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <20BE9059B6FC2D4B9E8FF2A9E2C4EEBD01F9FED2@HOS2KEXCHCL.howost.strykercorp.com> New Cold Jersey is 9 degrees, with minus 30 (windchills) and 5 inches of snow. Can't beat this cold weather... Stay warm, Nena Dimaano, MT/HT(ASCP) Advanced Technology Stryker, Orthopaedics Division 325 Corporate Drive Mahwah, NJ 07430 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com Sent: Friday, January 16, 2004 11:59 AM To: Weems, Joyce Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hope you all aren't freezing in the east! Chicago is a balmy 25, with freezing rain and sleet predicted for this evening. Perfect. Why do we live here? Does anyone have a scientific reason for temps to get to -100F (windchill is what I heard for the East). Don't bugs die when it's warmer? Don't some just stay frozen and pop back up again in the spring? I don't see any reason for it to be this cold!!! (I don't see a scientific reason to support the existence of mosquitoes, either) Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2004 10:39 AM To: "'Morken, Tim - Labvision'" , Histonet@lists.utsouthwestern.edu cc: Subject: RE: [Histonet] Hope you all aren't freezing in the east! But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will not blow down this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Fri Jan 16 12:04:46 2004 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <20040116.100516.490.921943@webmail25.nyc.untd.com> Well, here in North Texas, if you do not like the weather just wait a few minutes, it will change. We have been having unusually warm weather for this time of year. Last Friday about 80 but by Sunday it was down to 40. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From froyer <@t> bitstream.net Fri Jan 16 12:21:46 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Metric versus other units In-Reply-To: <3.0.6.32.20040116083241.00bd9f50@gemini.msu.montana.edu> References: <3.0.6.32.20040116083241.00bd9f50@gemini.msu.montana.edu> Message-ID: <40082BBA.6050800@bitstream.net> WOT! Now Mike, don't you know that it is the Brits & the Americans who are the ones that DO have it RIGHT! It's the rest of the world that is all goofy with that silly old metric system. You wouldn't even dream of asking your mate to meet you at the Pub after work for a few "473 mL'ers" now would you? That would be blasphemy! ;-) I agree with you that one should not try the igniting of wet gun powder with 100 proof alcohol (ethanol). Such a terrible waste of good sipping spirits. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, LLC Minneapolis, MN >>Dear All >> Brits and Americans are probably the worst >>offenders with Fahrenheit, gallons and many other old fashioned units still >>used. >> >>Just in case you need to check your processing solutions: 100 proof alcohol >>is the concentration of alcohol that will ignite wet gun powder, >> >>But seriously don't try this >> >>Happy New Year and >>Best Wishes >> >>Mike Bromley >> >>Chief Biomedical Scientist >>Pathology >>Dumfries & Galloway Royal Infirmary >>Scotland, UK >> >> From shive003 <@t> umn.edu Fri Jan 16 12:26:33 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] off-topic: weather talk Message-ID: <00dc01c3dc5e$44d0ff90$78065486@vdl220FAC> What with the unbelievably frigid weather out on the East Coast, I guess this means no more sarcastic jokes about winter in Minnesota from you folks. Today in St. Paul, it's 30 degrees ABOVE zero (Fahrenheit), and there's no snow on the grass lawns. It almost seems balmy! Or maybe that's me. Jan Shivers U of MN Vet Diag Lab St. Paul, MN, USA From mbryhan <@t> NORTHERNHEALTH.ORG Fri Jan 16 12:30:26 2004 From: mbryhan <@t> NORTHERNHEALTH.ORG (Mary Bryhan) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] off-topic: weather talk Message-ID: TGIF to all! It was -22 in Pellston, MI this morning! Have a good weekend! >>> "Jan Shivers" 01/16/04 01:26PM >>> What with the unbelievably frigid weather out on the East Coast, I guess this means no more sarcastic jokes about winter in Minnesota from you folks. Today in St. Paul, it's 30 degrees ABOVE zero (Fahrenheit), and there's no snow on the grass lawns. It almost seems balmy! Or maybe that's me. Jan Shivers U of MN Vet Diag Lab St. Paul, MN, USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Fri Jan 16 12:34:05 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Metric versus other units Message-ID: <494304423C63E246A5CF87A3AEEB577011B5D4@bumail01.barrynet.barry.edu> We used to finish off a long day at the lab with a schooner of Foster's. In spite of that, I still weigh only 11 1/2 stone (not bad for a guy who is just 4 barleycorns over 1 1/2 ells tall). If I keep up the long lab days, I may someday rate an office that larger than my present 1 rod long and 1/2 rod wide. I may also be able to get a car that won't occasionally force me to walk the 12 furlongs to the university. Allen A. Smith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, January 16, 2004 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Metric versus other units WOT! Now Mike, don't you know that it is the Brits & the Americans who are the ones that DO have it RIGHT! It's the rest of the world that is all goofy with that silly old metric system. You wouldn't even dream of asking your mate to meet you at the Pub after work for a few "473 mL'ers" now would you? That would be blasphemy! ;-) I agree with you that one should not try the igniting of wet gun powder with 100 proof alcohol (ethanol). Such a terrible waste of good sipping spirits. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, LLC Minneapolis, MN >>Dear All >> Brits and Americans are probably the worst >>offenders with Fahrenheit, gallons and many other old fashioned units >>still used. >> >>Just in case you need to check your processing solutions: 100 proof >>alcohol is the concentration of alcohol that will ignite wet gun >>powder, >> >>But seriously don't try this >> >>Happy New Year and >>Best Wishes >> >>Mike Bromley >> >>Chief Biomedical Scientist >>Pathology >>Dumfries & Galloway Royal Infirmary >>Scotland, UK >> >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From mrl0627 <@t> mail.ecu.edu Fri Jan 16 12:36:21 2004 From: mrl0627 <@t> mail.ecu.edu (mrl0627@mail.ecu.edu) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <18374450.1074278181796.JavaMail.SYSTEM@onestop1> Here in eastern North Carolina, went for my morning run wearing shorts 2 weeks ago---have needed 3 layers for single digit windchills since. Hurry summer!!! Maureen Loomer, East Carolina University -----Original Message----- ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Fri Jan 16 12:46:42 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! In-Reply-To: <9E75DAB5F369D84ABF84FAB7A0243B44019798E0@exch4.sjha.org> Message-ID: I don't want to rub it in but yesterday my husband and I went on a bike ride here in the Denver Colorado area rather than usual weekly cross-country ski trip in the mountains because it has been too warm and the snow wasn't any good. It has been in the 60's and even 70's here for about a week. It is a bit cooler today (48-50). This is not at all uncommon for Colorado. People think that it is cold here all winter but the fact of the matter that here on the plains in front of the mountains we have a very warm climate with more than 300 days per year of sunshine, but don't tell anyone we don't want anymore people moving here. Best regards,] Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, January 16, 2004 9:39 AM To: 'Morken, Tim - Labvision'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hope you all aren't freezing in the east! But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will not blow down this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From pruegg <@t> colobio.com Fri Jan 16 12:52:47 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] glycol methacrylate and sectioning In-Reply-To: <3.0.6.32.20040116085957.00bd9f50@gemini.msu.montana.edu> Message-ID: I agree. I never had any luck using disposable knives to cut even 5 micron sections in GMA although I seem to remember a tread a while ago where someone else said they used the disposables on GMA stuff. I guess it just depends on what knives, what microtome, what tissue, what blocks, what person, etc. In my hands the disposable blade holders did not hold tight enough for GMA sectioning. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Friday, January 16, 2004 9:00 AM To: Geoff McAuliffe; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] glycol methacrylate and sectioning Having done glycol methacrylate, I agree with Geoff's comments. I have a feeling 30 um sections of GMA embedded tissues would shatter coming off the knife, and you would probably need an expensive tungsten carbide knife to even attempt this and a really powerful sliding microtome. We always reserved GMA methods for 3 um or less thickness, thicker we went back to paraffin, OR with bone, ground and polished sections in methylmethacrylate, but this would NOT be suitable for brain work, rather mineralized bone sections. Good luck. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) At 10:16 AM 1/16/2004 -0500, you wrote: >Hi David: > > If you want 30 micron sections of brain why not frozen sections? or >celloidin sections? I have never heard of cutting glycol methacrylate >that thick, I always thought the purpose of plastic sections was thin >sections. Perhaps the "plastic" people on this list can provide advice. > >Geoff > >David Laidley wrote: > >>This may be a stupid question but can a sliding microtome cut brain tissue at 30 um when embedded in glycol methacrylate. I know that there exists rotory microtomes that cut glycol methacrylate but I can't seem to find any information about sliding microtomes. >> >>If you can cut 30 um sections of brain tissue in glycol methacrylate then does anyone have any tips on how this might be done (well). Or at least any tips or tricks you may have picked up over the years that may make the process go more smoothly. >> >>David Laidley (MSc student) >>Memorial University of Newfoundland >>Division of Basic Medical Sciences, Neuroscience >>dave_laidley@yahoo.ca >> >> >> >>--------------------------------- >>Post your free ad now! Yahoo! Canada Personals >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > >-- >-- >********************************************** >Geoff McAuliffe, Ph.D. >Neuroscience and Cell Biology >Robert Wood Johnson Medical School >675 Hoes Lane, Piscataway, NJ 08854 >voice: (732)-235-4583; fax: -4029 >mcauliff@umdnj.edu >********************************************** > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dobbin <@t> upei.ca Fri Jan 16 12:14:10 2004 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! In-Reply-To: Message-ID: <4007FFCC.6589.59A1E0E@localhost> Since we're comparing... Blizzard conditions and -40 degrees CELCIUS here on Canada's esat coast!! I'm loving it. Greg Date sent: Fri, 16 Jan 2004 09:23:56 -0800 From: Linke_Noelle Subject: RE: [Histonet] Hope you all aren't freezing in the east! To: "Morken, Tim - Labvision" , Histonet@lists.utsouthwestern.edu Copies to: > Tim is right. It's kind of cloudy here in southern California too, and > it's only supposed to get up to 70 today. Very sad. > > Noelle (damn glad she left Boston) Linke, BS, HTL(ASCP) > Allergan, Inc > 2525 Dupont Drive RD-2A > Irvine, CA 92612 > 714-246-5568 > > > -----Original Message----- > From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] > Sent: Friday, January 16, 2004 8:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hope you all aren't freezing in the east! > > I just want all of our collegues on the east coast (of US) to know we on > the > west coast empathize with your freezing cold weather and hope you get > through it OK. We are having really bad weather here too. It's cloudy > today. > > Tim Morken > Pleasanton (as nice as it sounds), California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Greg Dobbin Pathology Lab Atlantic Veterinary College, U.P.E.I. 550 University Ave. Charlottetown, P.E.I. Canada, C1A 4P3 Phone: (902)566-0744 Fax: (902)566-0851 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Making a living is getting; making a life is giving. From celebrej <@t> HHSC.CA Fri Jan 16 13:23:19 2004 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: <3AADFB88753AD31189C100902786B91C0E41C0D7@hch_nt_exchange.hhsc.ca> Here in Ontario Canada.... we are a balmy -20 degrees Celcius.... with a windchill factor of -35 degrees Celcius... not including the snow we were pummeled with.... not bad eh!! > ---------- > From: mprice26@juno.com[SMTP:mprice26@juno.com] > Sent: Friday, January 16, 2004 1:04 PM > To: Linke_Noelle@Allergan.com > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Hope you all aren't freezing in the east! > > > Well, here in North Texas, if you do not like the weather just wait a few > minutes, it will change. We have been having unusually warm weather for > this time of year. > > Last Friday about 80 but by Sunday it was down to 40. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From ljb <@t> medicine.wisc.edu Fri Jan 16 13:24:12 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Take care out there everyone, I'm in good old Madtown Wisconsin here. I know I sound old in saying this but the weather is far milder here than it was in the 70s when I was a kid. I kind of miss the winters I grew up with north of Green Bay! LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> 01/16/04 10:58AM >>> Chicago is a balmy 25, with freezing rain and sleet predicted for this evening. Perfect. Why do we live here? Does anyone have a scientific reason for temps to get to -100F (windchill is what I heard for the East). Don't bugs die when it's warmer? Don't some just stay frozen and pop back up again in the spring? I don't see any reason for it to be this cold!!! (I don't see a scientific reason to support the existence of mosquitoes, either) Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/16/2004 10:39 AM To: "'Morken, Tim - Labvision'" , Histonet@lists.utsouthwestern.edu cc: Subject: RE: [Histonet] Hope you all aren't freezing in the east! But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will not blow down this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Friday, January 16, 2004 11:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Fri Jan 16 13:37:29 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! In-Reply-To: <4007FFCC.6589.59A1E0E@localhost> References: <4007FFCC.6589.59A1E0E@localhost> Message-ID: <40083D79.40206@bitstream.net> VERY interesting that it is -40 degrees CELSIUS where you are in Canada.... -40 degrees CELSIUS == -40 degrees FAHRENHEIT (you can look it up) See... we are all using the same system after all!! ~ Ford ;-) Greg Dobbin wrote: >Since we're comparing... >Blizzard conditions and -40 degrees CELCIUS here on Canada's >esat coast!! I'm loving it. >Greg > From STEGTM <@t> samcstl.org Fri Jan 16 13:50:52 2004 From: STEGTM <@t> samcstl.org (Therersa Stegall) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! Message-ID: Lynne: Sorry about the possibility of losing your dog and husband. Cheer up, this is "abnormal" weather for you, and will likely not last for the entire winter. After being stationed at Minot AFB in North Dakota, and then living in northwestern Minnesota, I find it mildlly amusing that being cold is national news. 30 to 70 below windchills are par there, and the kids still stand at the bustops. We plugged our cars in to keep the oil from freezing in the motors, and just generally got to like the folks we lived with a lot because we saw them constantly. Hope the weather breaks soon for you all, snot-freezing cold is not pleasant! Peace, Terre >>> "Bell, Lynne" 1/16/2004 12:02:16 PM >>> Here in central Vermont it was a balmy minus 16 this morning with about 30 mph winds. Much warmer than yesterday morning however, which was minus 27 with 30 mph winds. My husband is threatening to take the dog and move to Florida!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peoshel <@t> wisc.edu Fri Jan 16 13:58:37 2004 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! In-Reply-To: References: Message-ID: Nice, mid-twenties, skate-sailers out on the frozen Lake Mendota. No snow, though, rats. But why so cold? Well, I think of a couple of reasons ... one, naturally (all together now) is global warming. The air heats up even more in the tropics and subtropics, especially over the oceans, and rises to the upper atmosphere, which is at lower pressure. The upper atmosphere is colder, and the air expands at the lower pressure, cooling it further. Global warming increases the amount of warm air rising to loose its heat. Looking at a general global circulation map, we find a general flow of air from the tropics to the higher latitudes in the upper atmosphere. This air gets even colder as it moves north (or south), then sinks, and there we are! lots more cold air sinking in the temperate latitudes because there's lots more hot air in the subtropics and tropics. Don't like that one? OK, how about basic physics. Heat is molecular motion, right? At normal pressures, molecules move very little before bouncing off of another molecule. These collisions transfer energy from one molecule to another, and the molecule that lost energy moves slower or cools. But! Following the Second Law of Thermodynamics, we find that there cannot be a 100% efficient transfer of energy, some is always lost, so the energy-receiving molecule in the example above is not warmed as much as the energy-donating molecule is cooled. As a result, the bulk temperature drops, and over time and all molecules, the population of molecules gets slower and slower, i.e. gets colder and colder. So it's really cold in the eastern US basically because their molecules are fatigued from all the jostling. Or how about ... You're right, it's the bugs. Many species do freeze in the winter, depending on natural cryoprotectants to survive the freezing. But, recall that orange growers in Florida spray water on their crop during a frost advisory to prevent freezing. Why? Latent heat -- the freezing water in the spray gives off heat as the water crystallizes, and this keeps the oranges above freezing. For the short while Florida gets freezing temperatures (which is why people in New Hampshire don't carry spray bottles outdoors in the winter). What does this have to do with bugs? After ice freezes, it's still water, with a high specific heat, and it takes up heat from the environment. Same with the bugs: Winter comes, they freeze, giving off latent heat and briefly warming their surroundings -- we call it Indian Summer -- and then, finally frozen, they suck all the rest of the heat out of things. So take your choice: global warming, tired molecules, or frozen bugs. Phil By the way, I've got this nice bridge in Brooklyn, if you're interested -- a bit icy, but only used by little old ladies on Sunday ... >Chicago is a balmy 25, with freezing rain and sleet predicted for this >evening. Perfect. Why do we live here? > >Does anyone have a scientific reason for temps to get to -100F (windchill >is what I heard for the East). Don't bugs die when it's warmer? >Don't some just stay frozen and pop back up again in the spring? I don't >see any reason for it to be this cold!!! (I don't see a scientific reason >to support the existence of mosquitoes, either) > >Jacqueline M. O'Connor HT(ASCP) >Abbott Laboratories >Global Pharmaceutical Research and Development >Discovery Chemotheraputics >847.938.4919 > > > > > >"Weems, Joyce" >Sent by: histonet-bounces@lists.utsouthwestern.edu >01/16/2004 10:39 AM > > > To: "'Morken, Tim - Labvision'" , >Histonet@lists.utsouthwestern.edu > cc: > Subject: RE: [Histonet] Hope you all aren't freezing >in the east! > > >But in Hotlanta we are in the sun and shirt sleeves. Hopefully it will >not >blow down this way! > >j > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, >Tim - Labvision >Sent: Friday, January 16, 2004 11:36 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] Hope you all aren't freezing in the east! > > >I just want all of our collegues on the east coast (of US) to know we on >the >west coast empathize with your freezing cold weather and hope you get >through it OK. We are having really bad weather here too. It's cloudy >today. > >Tim Morken >Pleasanton (as nice as it sounds), California > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message may >be privileged and is confidential information intended for the use of >the addressee listed above. If you are neither the intended recipient >nor the employee or agent responsible for delivering this message to the >intended recipient, you are hereby notified that any disclosure, >copying, distribution or the taking of any action in reliance on the >contents of this information is strictly prohibited. If you have >received this communication in error, please notify us immediately by >replying to the message and deleting it from your computer. >Thank you. Saint Joseph's Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From JWEEMS <@t> sjha.org Fri Jan 16 14:20:47 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] - Off Topic - Hope you all aren't freezing in the east! Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44019798FE@exch4.sjha.org> I have really enjoyed the chatter today! We should do more often! Have a good weekend everyone, j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Therersa Stegall Sent: Friday, January 16, 2004 2:51 PM To: Lynne.Bell@hitchcock.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hope you all aren't freezing in the east! Lynne: Sorry about the possibility of losing your dog and husband. Cheer up, this is "abnormal" weather for you, and will likely not last for the entire winter. After being stationed at Minot AFB in North Dakota, and then living in northwestern Minnesota, I find it mildlly amusing that being cold is national news. 30 to 70 below windchills are par there, and the kids still stand at the bustops. We plugged our cars in to keep the oil from freezing in the motors, and just generally got to like the folks we lived with a lot because we saw them constantly. Hope the weather breaks soon for you all, snot-freezing cold is not pleasant! Peace, Terre >>> "Bell, Lynne" 1/16/2004 12:02:16 PM >>> Here in central Vermont it was a balmy minus 16 this morning with about 30 mph winds. Much warmer than yesterday morning however, which was minus 27 with 30 mph winds. My husband is threatening to take the dog and move to Florida!! Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From jlinda <@t> ces.clemson.edu Fri Jan 16 15:07:42 2004 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Sectioning GMA with disposable blades! Message-ID: <5.1.0.14.2.20040116155715.02990840@mailhost.ces.clemson.edu> Dear Patsy, et al, That was me that mentioned sectioning GMA with disposable blades. I have a one-year old Leica 2155 automated microtome and I use the high profile disposable blades (NOT tungsten carbide). The speed is reduced to a VERY slow speed and the sections cut great ~ 4 micron. We are routinely using this for our tissue engineered scaffolds and cellular constructs. The engineering students much prefer using this method over the Polycut E. That all being said, I doubt that 30 microns would section in this manner. Also, it is necessary to wear safety glasses while sectioning just in case you hit an extra brittle area (first hand knowledge). Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From jnocito <@t> satx.rr.com Fri Jan 16 17:43:46 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] off-topic: weather talk References: <00dc01c3dc5e$44d0ff90$78065486@vdl220FAC> Message-ID: <009b01c3dc8a$9c225880$70494542@satx.rr.com> I knew there was reason why I moved to south Texas. Thunderstorms, flood watch and 65 degrees above 0. Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas ----- Original Message ----- From: "Jan Shivers" To: "histonet" Sent: Friday, January 16, 2004 10:26 AM Subject: [Histonet] off-topic: weather talk > What with the unbelievably frigid weather out on the East Coast, I guess > this means no more sarcastic jokes about winter in Minnesota from you folks. > > Today in St. Paul, it's 30 degrees ABOVE zero (Fahrenheit), and there's no > snow on the grass lawns. It almost seems balmy! > > Or maybe that's me. > > Jan Shivers > U of MN Vet Diag Lab > St. Paul, MN, USA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joeamateur <@t> hotmail.com Fri Jan 16 16:49:43 2004 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] off-topic: weather talk Message-ID: >I knew there was reason why I moved to south Texas. Thunderstorms, flood >watch and 65 degrees above 0. This is why it's good to be here in Honolulu. Our Stuff may be a bit pricier with shipping costs, but you can't beat the 76 F (24 C) degree weather. No storms at the moment, though two days ago was a doozy. If I could box up some warmth and aloha, I'd send it to all of you dear wonderful people, whether or not you're freezing at the moment. My warmest, toastiest wishes to all of you. Have a great weekend, and aloha to all! --Jack England _________________________________________________________________ Scope out the new MSN Plus Internet Software — optimizes dial-up to the max! http://join.msn.com/?pgmarket=en-us&page=byoa/plus&ST=1 From ekaplan <@t> squ.edu.om Fri Jan 16 22:12:52 2004 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Hope you all aren't freezing in the east! In-Reply-To: Message-ID: And here in the very east...........it rained heavily on Thurs (first time in 6 months) and temps went down to 15 deg C last night!! Lovely! Best wishes Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bartlett, Jeanine Sent: Friday, January 16, 2004 9:31 PM To: Morken, Tim - Labvision; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hope you all aren't freezing in the east! Hmmmm....Here in Atlanta it's chilly in the mornings (around 32) and lovely in the afternoon (around 55). Not as warm as California but fewer fires, earthquakes, floods and mudslides! Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim - Labvision Sent: Fri 1/16/2004 11:35 AM To: Histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Hope you all aren't freezing in the east! I just want all of our collegues on the east coast (of US) to know we on the west coast empathize with your freezing cold weather and hope you get through it OK. We are having really bad weather here too. It's cloudy today. Tim Morken Pleasanton (as nice as it sounds), California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SPARKYKA <@t> aol.com Sat Jan 17 07:41:08 2004 From: SPARKYKA <@t> aol.com (SPARKYKA@aol.com) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Re: Histonet Digest, Vol 2, Issue 18 Message-ID: <7e.4533ad25.2d3a9574@aol.com> From amosbrooks <@t> earthlink.net Sat Jan 17 12:33:46 2004 From: amosbrooks <@t> earthlink.net (amosbrooks) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] North East Climate Message-ID: <10501656.1074364426765.JavaMail.Administrator@atp> Hi, Although it is pretty cold and snowy here, I wouldn't have traded sledding with my 6 year old daughter Meghan this afternoon for anything. If you're having fun you hardly notice the cold. When you do feel the cold just remember how fun it was teaching her to swim in pool in blazing heat of July. Shivering in CT, Amos Brooks From jqb7 <@t> cdc.gov Sat Jan 17 12:28:43 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] North East Climate Message-ID: I'm with you! I'd give anything to have more snow days down here in Atlanta! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of amosbrooks Sent: Saturday, January 17, 2004 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] North East Climate Hi, Although it is pretty cold and snowy here, I wouldn't have traded sledding with my 6 year old daughter Meghan this afternoon for anything. If you're having fun you hardly notice the cold. When you do feel the cold just remember how fun it was teaching her to swim in pool in blazing heat of July. Shivering in CT, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From georgecole <@t> ev1.net Sun Jan 18 10:24:24 2004 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:26 2005 Subject: [Histonet] Moral Imperatives in biopsy processing Message-ID: <000001c3dddf$8a613de0$064dbad0@hppav> Histotechs: 97 packets of new muscle and nerve biopsy methods have been sent so far to 33 states and 16 countries outside the US since last July. I really didn't dream there would be such a response to my offer. It has begun to grow a greater significance---the dawning of a kind of MORAL IMPERATIVE here----I mean that finding improvements in one's methods of service to a patient, -makes the performance of the improved method and replacing the less adequate method a no-choice matter: there can be no question about continuing practices if they are truly inferior to other known methods that are truly more effective. This is life and death stuff. This is not one's golf swing or dance style. This is what it is to be involved in the biopsy process---we are in the front lines in the patient's fight for a diagnosis of disease or wellness, decline or improvement---and, so we are often involved in maintaining life itself. When an improvement was made to the searching out the information in biopsy tissues, it was immediately put into the rotuine of was found of biopsy patients. Let us beseech each other to keep us at the good efforts to contribute something in muscle and nerve biopsy service to the people who need their muscle and nerve tissues searched for the good or the ill in them. georgecole@ev1.net . From siksik03 <@t> comcast.net Sun Jan 18 13:19:50 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Sunday Humor In-Reply-To: <400807D2.5020302@pathology.washington.edu> References: <400807D2.5020302@pathology.washington.edu> Message-ID: Hi HistoNetters I heard of a blonde that thought that Taco Bell was the national phone company of Mexico. Steven At 7:48 AM -0800 1/16/04, Victor Tobias wrote: >This is courtesy of my blonde wife. Last night I finally ported our >cell numbers from AT&T to Verizon. After being home for awhile my >wife asked how close were the new numbers to our old number. From caroline.stott <@t> anatomy.otago.ac.nz Sun Jan 18 13:58:39 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] glycol methacrylate and sectioning Message-ID: <5.2.1.1.0.20040119085326.0290cb30@anatomy.otago.ac.nz> Hi, we regularly cut thick Glycol methacrylate sections. I am doing some right now in fact. We apply a drop of water using a cotton bud to soften the surface of the resin and also create a small puddle on the glass knife itself. They come off fine. We then sink the sections to the bottom of a cold water bath (instead of floating them on top like thin sections) pick them up onto the slide, and place a wet piece of filter paper over the sections and use a roller to firmly stick the sections to the slide. Next we put the sections onto a hot plate around 60-70 degrees C for approximately 10 minutes , this ensures a firm stick. If they start to lift off, roll them again. A lot of people use thick sections for stereology. Hope that helps. Caroline Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From JWEEMS <@t> sjha.org Sun Jan 18 21:21:02 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Sunday Humor Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B440197990A@exch4.sjha.org> You mean its not?! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: Victor Tobias; Histonet@lists.utsouthwestern.edu Sent: 1/18/2004 2:19 PM Subject: Re: [Histonet] Sunday Humor Hi HistoNetters I heard of a blonde that thought that Taco Bell was the national phone company of Mexico. Steven At 7:48 AM -0800 1/16/04, Victor Tobias wrote: >This is courtesy of my blonde wife. Last night I finally ported our >cell numbers from AT&T to Verizon. After being home for awhile my >wife asked how close were the new numbers to our old number. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From anaflorenti <@t> yahoo.com Mon Jan 19 06:44:59 2004 From: anaflorenti <@t> yahoo.com (Ana Florentin) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] BrdU/DAPI and BrdU/hematoxylin counterstaining Message-ID: <20040119124459.65400.qmail@web11903.mail.yahoo.com> Hello everybody! I am trying to use the BrdU staining to look at proliferation in frozen rat aortic sections. Detection of incorporated BrdU, by using monoclonal antibodies directed against BrdU, is only feasible in single stranded DNA. Therefore this technique requires a pretreatment to denature double stranded DNA. I am using 2 M HCl at 37ºC for 15 minutes and I obtained positive labeled cells in thymus (used as positive controls) as long as in the experimental aorta sections. My interest is to count de BrdU positive cells and to quantify them as mean percentage of positive cells. In this regard, I need to easily discriminate the labeled cells from the unlabeled nuclei in the same microscopic fields. My problem is that I can not perform a double immunofluorescence staining BrdU/DAPI of the nuclei. The HCl pretreatment made the DAPI staining giving only a fuzzy blue background, thus I was unable to detect the nuclei. I also tried the immunoperoxidase staining (which also worked for BrdU) and counterstaining with hematoxylin, with the same pretreatment, results and…problem. I know from literature that the hematoxylin counterstaining should work, even if there is the HCl pretreatment. But, in my hands, the supposed hematoxylin stained nuclei could not be clearly identified. I would be really grateful for any suggestions or comments. Thank you and all my best! Anna --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From DPALLP <@t> aol.com Mon Jan 19 08:52:18 2004 From: DPALLP <@t> aol.com (DPALLP@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] job opening in Southeast Texas Message-ID: <0C80602B.087AFE39.00042A59@aol.com> Diagnostic Pathology Associates, LLP in Beaumont, Texas has an opening for a qualified histology technician. If interested, please fax resume to 409-842-8244. Susie Duhon, HT(ASCP) QIHC Diagnostic Pathology Associates, LLP Beaumont, Texas 409-842-8222 409-842-8244 (fax) dpallp@aol.com From Janet.Bonner <@t> FLHOSP.ORG Mon Jan 19 09:37:41 2004 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] off-topic: weather talk Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F45@fh2k093.fhmis.net> Low 70's - high 60's here in Orlando, FL - We'll pray for ya'll! -----Original Message----- From: Mary Bryhan [mailto:mbryhan@NORTHERNHEALTH.ORG] Sent: Friday, January 16, 2004 1:30 PM To: histonet@lists.utsouthwestern.edu; shive003@umn.edu Subject: Re: [Histonet] off-topic: weather talk TGIF to all! It was -22 in Pellston, MI this morning! Have a good weekend! >>> "Jan Shivers" 01/16/04 01:26PM >>> What with the unbelievably frigid weather out on the East Coast, I guess this means no more sarcastic jokes about winter in Minnesota from you folks. Today in St. Paul, it's 30 degrees ABOVE zero (Fahrenheit), and there's no snow on the grass lawns. It almost seems balmy! Or maybe that's me. Jan Shivers U of MN Vet Diag Lab St. Paul, MN, USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From franktringale <@t> comcast.net Mon Jan 19 11:53:31 2004 From: franktringale <@t> comcast.net (Frank Tringale) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Newly designed Labs Message-ID: <652E58B7-4AA8-11D8-89BA-000A95C5266A@comcast.net> Stanford Medical Center is in the process of designing a new Anatomical and Clinical Laboratory. We are are currently working with architects with ideas for the new labs. This is going to be a huge project for us and we want it to be "state of the art". If any of you have gone through this process I would appreciate any suggestions that you may have. Also, if there is a lab that has been recently built or redesigned, I would like to make a site visit, if possible. Thank you in advance. Frank Tringale HT, HTL Supervisor of Histology Services Department of Surgical Pathology Stanford University Medical Center 650-725-2350 From SCheasty <@t> ahs.llumc.edu Mon Jan 19 12:34:29 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] CLIA eligible Grossers - What should they be paid? Message-ID: <2E50F33F91EEDA46A77BC3B2575BB091058736@mars.llumc.edu> * I'm not talking about certified P.A.'s who complete an accredited program and who are capable of doing entire autopsies. * I'm talking about CLIA-eligible staff who train on-the-job for grossing that institution's specimens. * What should they get paid in comparison to certified histologists at that institution? * What if they are certified histologists as well? Should they get paid more than a certified Histologist who doesn't/can't do the grossing? Any input is most appreciated. Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From JWEEMS <@t> sjha.org Mon Jan 19 14:08:05 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B4401979923@exch4.sjha.org> An unofficial survey: How many hospitals are working Saturdays? And what is the case load of those that do? Thanks for your help! Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From JMVICTO <@t> arborgen.com Mon Jan 19 14:49:22 2004 From: JMVICTO <@t> arborgen.com (Jerrin Victor) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Help with histology... Message-ID: Hi! I am a new member to this group. I am interested in getting some Histology work done to study anatomical differences among normal and abnormal Pine somatic embryos. Is there a company or departments in universities out there who does Histology work as a service to outside clients?? Any information on this will be greatly appreciated.. I am stationed in South Caroline, USA. Thanks Jerrin. From barbalb <@t> itsa.ucsf.edu Mon Jan 19 14:51:20 2004 From: barbalb <@t> itsa.ucsf.edu (barbalb@itsa.ucsf.edu) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: <200401192051.i0JKpKF13192@itsa.ucsf.edu> UCSF Medical Center, San Francisco here. We have one person working Saturdays. Embed, cut, stain (and process, if necessary) any rush biopsy (usually those with suspected transplant rejection), embed 200-500 routine surgicals, then organize them (using Co-Path) and print labels (again using Co-Path) so they're ready to cut on Monday morning. That plus clean-up usually takes all day. Barbara Albert From HornHV <@t> archildrens.org Mon Jan 19 15:11:33 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: We do not work Saturdays unless there is an emergency case that cannot wait until Monday. This happens about 4 times a year. And if we come in that is the only case we do. We are on-call for long weekend holidays. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Monday, January 19, 2004 2:08 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Working Saturdays An unofficial survey: How many hospitals are working Saturdays? And what is the case load of those that do? Thanks for your help! Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From BlazekL <@t> childrensdayton.org Mon Jan 19 15:34:10 2004 From: BlazekL <@t> childrensdayton.org (Linda Blazek) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: We do not work Saturdays unless there is an emergency case that cannot wait until Monday. This doesn't happen very often. We rotate call and cover all weekends and holidays. Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center 1 Children's Plaza Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl@childrensdayton.org >>> "Weems, Joyce" 01/19/04 03:08PM >>> An unofficial survey: How many hospitals are working Saturdays? And what is the case load of those that do? Thanks for your help! Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From laurie.colbert <@t> huntingtonhospital.com Mon Jan 19 15:48:45 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BD73@EXCHANGE1.huntingtonhospital.com> We work Saturdays and it is often the busiest day of the week for us. The case load varies but we probably average 200-250 blocks. We have one lab assistant and three histotechs working. -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Monday, January 19, 2004 12:08 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Working Saturdays An unofficial survey: How many hospitals are working Saturdays? And what is the case load of those that do? Thanks for your help! Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From histomjans <@t> yahoo.com Mon Jan 19 17:26:03 2004 From: histomjans <@t> yahoo.com (Melissa Jans) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] working saturdays Message-ID: <20040119232603.35621.qmail@web14912.mail.yahoo.com> Joyce, We have a tech that works each Saturday, it is assigned on a rotating basis and is also the same person who is on call for that particular weekend. They embed, cut and stain 65 blocks in a 4 hour time frame. Melissa Jans University of Iowa Hospitals and Clinics --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From Instaken <@t> aol.com Mon Jan 19 19:22:21 2004 From: Instaken <@t> aol.com (Instaken@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] HPV by ISH question Message-ID: <1cc.17fdcbf3.2d3ddccd@aol.com> Hello all: Does anyone know what level of professional reads/screens ThinPrep Pap tests for HPV by ISH? I have in mind the Ventana Benchmark autoimmunostainer test for HPV. Do histo or cyto techs prescreen these and send them to a pathologist or can these be "signed out" by someone other than a pathologist? Many thanks, K Whittenburg From micro <@t> formatex.org Mon Jan 19 19:01:48 2004 From: micro <@t> formatex.org (Microscopy Book Series) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] CALL FOR CHAPTERS for Multidisciplinary Microscopy Book Message-ID: <00ac01c3def1$04432620$0e001aac@LocalHost> Dear Colleague, Formatex, a Spanish Research Center, in association with Kluwer Academic Publishers, is now preparing the Second number of the Formatex Microscopy book series, with the preliminary title of "Current Issues in Multidisciplinary Microscopy Research and Education". You can see the contents of the first number published in 2003 in http://www.formatex.org/micro2002/callforpaper.htm Website of the 2004 edition is http://www.formatex.org/micro2003/callforpaper2.htm This second number of the series is committed to giving an overview of the state of the art as well as upcoming trends, and to promoting discussion about scientific, technological and educational aspects of Microscopy in both the biological/biomedical and physical/chemical sciences. Although all types of papers are a priori accepted (research articles, reviews, case studies, etc.), priority will be given to those which clearly emphasize the scientific/technological/pedagogical results, as well as those making comparative discussions of two or more microscopy techniques or showing the complementarity of microscopy techniques with other techniques. For this second number, "educationally-oriented" and mini-review papers are specially welcome, although also "regular" research papers are accepted. If you are interested in participating in this edition submitting a (technical, scientific, educational, introductory...) chapter related to microscopy, please see the website for details. As you may see from the Call for Papers' website, the deadline for chapter submission is MARCH 15TH. Early submission of a short abstract of your chapter proposal is appreciated in order to allow potencial authors to know what other authors will write about for the book and avoid contents duplications. We hope that you find this new approach to microscopy issues interesting and we hope to hear from you/your team for this and/or future editions. If you any enquiry or suggestion about this volume, please contact us. Best wishes from Spain. Jos? Antonio Mesa Gonzalez Editorial Assistant Formatex Research Center C / Encarnacion, 3 1?E 06001 Badajoz SPAIN Phone/Fax: +34 924258615 Email: micro@formatex.org From CrochiereSteve <@t> aol.com Mon Jan 19 19:48:53 2004 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: <191.243e063d.2d3de305@aol.com> Joyce, My lab does not have a Saturday schedule, however I am on-call most weekends for autopsy deiner. I do work Saturday mornings at another hospital where I embed and cut only (about 100 - 200 blocks embed - 60 - 80 slides cut) . I may need to start Saturday hours in my own lab, but would probably start with a rotating schedule shared by myself & the other 3 techs. Steven M. Crochiere, HT (ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center 299 Carew St. Springfield, MA 01104 From CrochiereSteve <@t> aol.com Mon Jan 19 19:51:01 2004 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] CLIA eligible Grossers - What should they be paid? Message-ID: <19d.1f852606.2d3de385@aol.com> Sandy, Yes they probably should get paid more, but in my 23 years experience I've grossed many a specimen as "Part of the job." One does what one must to ensure the job gets done. Steven M. Crochiere, HT (ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center 299 Carew St. Springfield, MA 01104 From juan.gutierrez <@t> christushealth.org Tue Jan 20 07:25:21 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: Hello Joyce. We work every Saturday with workloads ranging from 20 -120 blocks. I have one tech that embeds and covers the gross room. I also have another tech that comes in and does the cutting, staining and a few specials(bug stains only). We do not run immuno or liver and kidney panels on Saturdays. Both techs are here from 4-12. The cutter position is on a rotation schedule, the embedder is a part-timer. Hope this helps. Juan -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Mon 1/19/2004 2:08 PM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] Working Saturdays An unofficial survey: How many hospitals are working Saturdays? And what is the case load of those that do? Thanks for your help! Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From Vickroy.Jim <@t> mhsil.com Tue Jan 20 07:40:02 2004 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carryover on slides Message-ID: <584AE8968B1EE14998210A0D6B97D854055AEE64@mmcmail2.mhsil.com> Has anyone ever seen any articles on what is an acceptable rate of carryover on routine slides? We have been monitoring our carryover rate and our pathologists wondered if anyone had ever seen a study on carryover rates. James R. Vickroy BS, HT (ASCP) Technical Supervisor, Surgical Pathology 788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Ngilman <@t> nbhd.org Tue Jan 20 07:48:01 2004 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carryover on slides Message-ID: Jim, what do you mean by "carryover"? Can you be more specific? Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager >>> "Vickroy, Jim" 1/20/2004 8:40:02 AM >>> Has anyone ever seen any articles on what is an acceptable rate of carryover on routine slides? We have been monitoring our carryover rate and our pathologists wondered if anyone had ever seen a study on carryover rates. James R. Vickroy BS, HT (ASCP) Technical Supervisor, Surgical Pathology 788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** From Dave.Pizi <@t> carolinashealthcare.org Tue Jan 20 07:08:12 2004 From: Dave.Pizi <@t> carolinashealthcare.org (Pizi, Dave) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Working Saturdays Message-ID: <7E22BEDC45D51449B847E15983E99BAD721C91@dcr-xchg-04.Carolinas.org> Joyce We have 2 techs working a full shift (usually a little more) and a lab assistant works half a day on Saturdays. They embed about 350 to 450 blocks, cut and stain 20-40 rush blocks for the docs and than just cut anything else they can to get a head start for Monday. Dave Pizi > -----Original Message----- > From: Weems, Joyce [SMTP:JWEEMS@sjha.org] > Sent: Monday, January 19, 2004 3:08 PM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Working Saturdays > > > An unofficial survey: > > How many hospitals are working Saturdays? And what is the case load of > those > that do? > > Thanks for your help! > > Joyce > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph's Health System, Inc. > << File: ATT97920.txt >> From rocan <@t> mac.com Tue Jan 20 08:19:10 2004 From: rocan <@t> mac.com (rocan) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] pepsin In-Reply-To: References: Message-ID: <9D40E466-4B53-11D8-8168-000A958A30B2@mac.com> Try Worthington http://www.worthington-biochem.com/default.html Rocio Honigmann Cedars-Sinai Medical Center On Jan 13, 2004, at 11:19 PM, Kirbi? Srebotnik Irena wrote: > Dear histoneters, > we used to use Pepsin (porcine) from Serva:1 milliAnson U/mg, EC 3.4 > 23 1, > Mr ca. 36 000 which is no more available, is someone familiar with this > activity unit for pepsin?, what kind of pepsin can we use instead? > treatment with pepsin is a part of FNAB sample processing procedure > for the > DNA measurements by flow cytometer. > > thanks for help > Irena Kirbis > Cytopathology Department > Institute of Oncology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jan 20 08:44:51 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carryover on slides In-Reply-To: <584AE8968B1EE14998210A0D6B97D854055AEE64@mmcmail2.mhsil.co m> Message-ID: <3.0.6.32.20040120074451.00bea4e8@gemini.msu.montana.edu> Could you define carryover? Do you mean cells, tissue fragments, stains from one staining dish to another? At 07:40 AM 1/20/2004 -0600, you wrote: >Has anyone ever seen any articles on what is an acceptable rate of carryover >on routine slides? We have been monitoring our carryover rate and our >pathologists wondered if anyone had ever seen a study on carryover rates. > > >James R. Vickroy BS, HT (ASCP) >Technical Supervisor, Surgical Pathology >788-4046 >vickroy.jim@mhsil.com > >This message (including any attachments) contains confidential information >intended for a specific individual and purpose, and is protected by law. If >you are not the intended recipient, you should delete this message. Any >disclosure, copying, or distribution of this message, or the taking of any >action based on it, is strictly prohibited. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Myri37 <@t> aol.com Tue Jan 20 08:58:16 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] antibody for bone alkaline phosphatase Message-ID: <1509D822.767EFC64.0005167B@aol.com> Hi everyone does anyone know a reference of bone alkaline phosphatase antibody can we use it on paraffin or MMA embedded samples ? thank you very much for your help myriam From Terry.Marshall <@t> rothgen.nhs.uk Tue Jan 20 09:00:59 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carryover on slides Message-ID: Surely all are examples of carryover. Carryover is anything that "didn't ought to be there" and has come from another specimen. It must be difficult to monitor, and the most difficult (i.e. impossible) is the like-to-like tissue carryover, that is to say, a bit of A's endometrium appearing on B's slide. Cut-up and water bath are the most likely origins, and can be minimised by scrupulous cleanliness. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 20 January 2004 14:45 To: Vickroy, Jim; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] carryover on slides Could you define carryover? Do you mean cells, tissue fragments, stains from one staining dish to another? At 07:40 AM 1/20/2004 -0600, you wrote: >Has anyone ever seen any articles on what is an acceptable rate of carryover >on routine slides? We have been monitoring our carryover rate and our >pathologists wondered if anyone had ever seen a study on carryover rates. > > >James R. Vickroy BS, HT (ASCP) >Technical Supervisor, Surgical Pathology >788-4046 >vickroy.jim@mhsil.com > >This message (including any attachments) contains confidential information >intended for a specific individual and purpose, and is protected by law. If >you are not the intended recipient, you should delete this message. Any >disclosure, copying, or distribution of this message, or the taking of any >action based on it, is strictly prohibited. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Jan 20 09:07:11 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carryover on slides Message-ID: Drat! I meant to say "... bit of A's endometrium appearing on B's slide of endometrium." Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist Sent: 20 January 2004 15:01 To: Gayle Callis; Vickroy, Jim; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] carryover on slides Surely all are examples of carryover. Carryover is anything that "didn't ought to be there" and has come from another specimen. It must be difficult to monitor, and the most difficult (i.e. impossible) is the like-to-like tissue carryover, that is to say, a bit of A's endometrium appearing on B's slide. Cut-up and water bath are the most likely origins, and can be minimised by scrupulous cleanliness. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 20 January 2004 14:45 To: Vickroy, Jim; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] carryover on slides Could you define carryover? Do you mean cells, tissue fragments, stains from one staining dish to another? At 07:40 AM 1/20/2004 -0600, you wrote: >Has anyone ever seen any articles on what is an acceptable rate of carryover >on routine slides? We have been monitoring our carryover rate and our >pathologists wondered if anyone had ever seen a study on carryover rates. > > >James R. Vickroy BS, HT (ASCP) >Technical Supervisor, Surgical Pathology >788-4046 >vickroy.jim@mhsil.com > >This message (including any attachments) contains confidential information >intended for a specific individual and purpose, and is protected by law. If >you are not the intended recipient, you should delete this message. Any >disclosure, copying, or distribution of this message, or the taking of any >action based on it, is strictly prohibited. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judy.stephens <@t> syngenta.com Tue Jan 20 09:08:46 2004 From: judy.stephens <@t> syngenta.com (judy.stephens@syngenta.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] hair sectioning Message-ID: <7D28784DD2EBD511AC4900508B6219C1033252CF@ukapagmbx01.ukct.zeneca.com> Does anyone have any knowledge of routine wax processing of human hair for immuno staining. Cutting at 2.5microns. Judy Stephens, Syngenta Cheshire, England From briany <@t> ufl.edu Tue Jan 20 09:32:40 2004 From: briany <@t> ufl.edu (Brian Hatcher) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Quantitative von Kossa Message-ID: <200401201532.i0KFWeAW092584@smtp.ufl.edu> Is anyone aware of a technique/references on extracting quantitative information from a von Kossa stain. I am interested in trying to measure the rate and extent of mineralization in a marrow stromal cell culture, and would like to know if this is possible using this stain, and also what target values I should be trying to achieve. Thanks. Brian Hatcher Graduate Research Asst. Dept. of Biomedical Engineering University of Florida From garygill <@t> dcla.com Tue Jan 20 08:59:08 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carryover on slides Message-ID: Regardless, how do you measure it? In terms of: fouling of rinses and frequency of replacement, cellular cross-contamination and false positives? Acceptable compared to what? And what's the motivation behind the question? Gary Gill -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Tuesday, January 20, 2004 9:45 AM To: Vickroy, Jim; Histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] carryover on slides Could you define carryover? Do you mean cells, tissue fragments, stains from one staining dish to another? At 07:40 AM 1/20/2004 -0600, you wrote: >Has anyone ever seen any articles on what is an acceptable rate of >carryover on routine slides? We have been monitoring our carryover >rate and our pathologists wondered if anyone had ever seen a study on >carryover rates. > > >James R. Vickroy BS, HT (ASCP) >Technical Supervisor, Surgical Pathology >788-4046 >vickroy.jim@mhsil.com > >This message (including any attachments) contains confidential >information intended for a specific individual and purpose, and is >protected by law. If you are not the intended recipient, you should >delete this message. Any disclosure, copying, or distribution of this >message, or the taking of any action based on it, is strictly >prohibited. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jlinda <@t> ces.clemson.edu Tue Jan 20 10:25:12 2004 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: <5.1.0.14.2.20040120111934.05634b88@mailhost.ces.clemson.edu> Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From SCheasty <@t> ahs.llumc.edu Tue Jan 20 10:39:18 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: <2E50F33F91EEDA46A77BC3B2575BB091058739@mars.llumc.edu> Frank, The biggest mistakes I've seen in designing labs or Information Systems is not including the end-user's input. Often the pathologists will have the last say, but they are not the ones who are processing the specimens from start to finish. You can avoid a lot of expensive changes after the fact, (and a lot of hurt feelings from staff) by having an avenue for staff to give input and keeping everyone aware of how things are proceeding. Whether the ideas are good and used, or not-so-good and discarded, I have found it worthwhile to include input from everyone. Also, if you have access to Microsoft Visio, (sort of a simple CAD software program) you can lay out the space easily and throw in counters and sinks and fume hoods and desks and re-arrange them in endless ways to give you ideas. Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 08:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From Loralee_Gehan <@t> URMC.Rochester.edu Tue Jan 20 10:45:19 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Pecam-1 (CD31) Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804BD@exmc3.urmc.rochester.edu> Has anyone out there come across a CD31 that works in formalin fixed paraffin embedded mouse tissue? Any retrieval methods used would help also. There are about 200 CD31's for human tissue. Can't seem to find one that works in mouse. Thanks a lot. Loralee Gehan Orthopaedics Research Lab From james.zimmerman <@t> pharma.novartis.com Tue Jan 20 10:32:46 2004 From: james.zimmerman <@t> pharma.novartis.com (james.zimmerman@pharma.novartis.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Uranyl Nitrate Message-ID: Hello, Has anyone recently ordered uranyl nitrate? If so, could you please forward me the name and address of the vendor. Thanks, JPZ From StarkusL <@t> ummhc.org Tue Jan 20 10:51:40 2004 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: Sandra makes an excellent point here. I'm currently working in a Mohs lab that was just designed three years ago. Because no one thought to ask me (the only tech working here), no one considered that a cryostat generates a LOT of heat as it cools. Consequently, we are running a room air conditioner 24 hours a day, 7 days a week, 365 days a year. Yes, it was -10 outside this past week, and we are running an air conditioner. And, you still can't close the door or the air conditioner can't keep up. Also, they forgot to include room for the explosives cabinet (for ethanol) and our microscope is behind the door. So, when someone is using the microscope, the door isn't all the way open. Our surgeon sometimes gets hit in the butt with the door as people try to get into the lab. -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Tuesday, January 20, 2004 11:39 AM To: Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Frank, The biggest mistakes I've seen in designing labs or Information Systems is not including the end-user's input. Often the pathologists will have the last say, but they are not the ones who are processing the specimens from start to finish. You can avoid a lot of expensive changes after the fact, (and a lot of hurt feelings from staff) by having an avenue for staff to give input and keeping everyone aware of how things are proceeding. Whether the ideas are good and used, or not-so-good and discarded, I have found it worthwhile to include input from everyone. Also, if you have access to Microsoft Visio, (sort of a simple CAD software program) you can lay out the space easily and throw in counters and sinks and fume hoods and desks and re-arrange them in endless ways to give you ideas. Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 08:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Tue Jan 20 10:55:40 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: At my old hospital when designing the lab somehow the blueprints were flipped and ventalation ducts ended up next to the cutting stations. Because the contractors wouldn't let us in the lab area until they were finished and our supervisor at the time didn't insist on inspecting the area, this wasn't noticed until they told us we could move in. Obviously they had to make a change before we could move in, costing money and time. Once construction starts inspect the area. Make sure you have authority to make changes after they start. It is likely that once things start to get in place you will see problems that are easier to correct in the middle of the renovation than after the renovation. Good luck. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson_jm <@t> cimar.org Tue Jan 20 11:03:09 2004 From: wilson_jm <@t> cimar.org (Jonathan Wilson) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] microtubes for aliquoting antibodies References: Message-ID: <01bb01c3df77$48058750$17dea8c0@ciimar.up.pt> Hello, I was wondering if there was anyone out there that could recommend a source (preferably in Europe) for plastic microtubes for aliquoting antibody sera (10-100ul) for storing in the freezer (to avoid freeze-thaw). I have recently been using PE beckman type tubes (0.4ml, 4 cm long) but I find them a bit bulky and the lids done seal so great. In the past (when I was a student in Canada), I was using shorter PP tubes with a maximum volume of 100-200 ul. I found that these softer tubes had better sealing lids. For aliquoting sera in such small volumes they were vastly superior to regular eppendorf type bullet tubes. Thanks for any suggestions. Sincerely, Jonathan CIIMAR-Ecofisiologia Rua dos Bragas 289 4050-123 Porto Portugal tel 351 22 340 1809 fax 351 22 339 0608 From tbailey <@t> uab.edu Tue Jan 20 11:08:26 2004 From: tbailey <@t> uab.edu (Tammy Bailey) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs In-Reply-To: <5.1.0.14.2.20040120111934.05634b88@mailhost.ces.clemson.edu> Message-ID: <00d501c3df78$0482b3d0$0f9c1a8a@tbailey1> We are in the middle of phase 2 of lab renovations here..We have a 2 Photon Laser Confocal Scope and the "german" plugs and the back up system are causing us lots of agony!! Make sure your architect understands your needs and concerns for back up power!! tam Tammy M. Bailey University of Alabama-Birmingham Histology Analysis Module Manager Vision Science Research Center 924 18th St. South Birmingham, AL 35294 205.934.3074 205.934-5725-fax tbailey@uab.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angela.mcnabola.b <@t> bayer.com Tue Jan 20 11:07:43 2004 From: angela.mcnabola.b <@t> bayer.com (Angela McNabola) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Pecam-1 (CD31) Message-ID: Hi, We use Santa Cruz PECAM-1 M-20 (SC-15060) goat polyclonal. We steam for 30 minutes in Dako TRS (S1699) for 30 minutes (cool to room temperature). We also avidin/biotin block (Vector), and use ABC (Vector elite kit). Works great, especially on mouse xenografts. Let me know if you need more information. Angela McNabola, MS, HT(ASCP)SLS Bayer Corp. West Haven, CT From John.Auld <@t> whnt.nhs.uk Tue Jan 20 11:21:25 2004 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: acceptable rate of carryover Message-ID: Jim I have a (very) vague recollection of a study done on carryover on an automated stainer for cervical cytology many many years ago. Don't remember what it said. BUT IMO there is only one acceptable rate for carryover and that is ZERO. This may not always be possible but that is what we should always work to. In my lab any slide with possible tissue carryover is always recut even if the suspect part is away from the section. regards John John Auld FIBMS MSc Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral Date: Tue, 20 Jan 2004 07:40:02 -0600 From: "Vickroy, Jim" Subject: [Histonet] carryover on slides To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <584AE8968B1EE14998210A0D6B97D854055AEE64@mmcmail2.mhsil.com> Content-Type: text/plain; charset="iso-8859-1" Has anyone ever seen any articles on what is an acceptable rate of carryover on routine slides? We have been monitoring our carryover rate and our pathologists wondered if anyone had ever seen a study on carryover rates. James R. Vickroy BS, HT (ASCP) Technical Supervisor, Surgical Pathology 788-4046 vickroy.jim@mhsil.com From Jackie.O'Connor <@t> abbott.com Tue Jan 20 11:19:46 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: Many many years ago - we started our first day in a newly built hospital histo lab just to realize that we had all the bench top jets we needed, i.e., air, gas, (we used a flame to embed with then) vacuum, water - but no one put in the 'guts' for any of those features - just the jets - for decoration, I suppose. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Stapf, Ross" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/20/2004 10:55 AM To: cc: Subject: RE: [Histonet] Re: Newly designed Labs At my old hospital when designing the lab somehow the blueprints were flipped and ventalation ducts ended up next to the cutting stations. Because the contractors wouldn't let us in the lab area until they were finished and our supervisor at the time didn't insist on inspecting the area, this wasn't noticed until they told us we could move in. Obviously they had to make a change before we could move in, costing money and time. Once construction starts inspect the area. Make sure you have authority to make changes after they start. It is likely that once things start to get in place you will see problems that are easier to correct in the middle of the renovation than after the renovation. Good luck. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Tue Jan 20 11:30:42 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: acceptable rate of carryover Message-ID: Husain OA, Grainger JM, Sims J. Cross contamination of cytological smears, with automated staining machines and bulk manual staining procedures. With a specific study of the problems of the Cytotek and the Shandon Elliott staining machines. J Clin Pathol. 1978 Jan; 31(1): 63-8. Further development of an individual staining machine is to be strongly encouraged but meanwhile, using bulk stainers, frequent changing of wash fluids and staining solutions, particularly leading up to and following the haematoxylin pot, is essential to reduce the risk of cross contamination. Certain smears, such as from semen or from serous fluids where malignancy is suspected or known, must be stained on separate racks. In some laboratories it is the rule not to stain semen or serous fluids in bulk staining machines at all and this may have to become the rule everywhere until we are provided with safe individual slide stainers. The Ames Cytotek moved Pap smears cellside-down across a stainless steel platen with grooves through which stain and rinses were metered. Manufacturer claimed there was a "capillary gap" that prevented cells from being abraded from one slide and attaching to another. Dr. Husain stained a set of Pap smears with a series of single blank slides between each Pap smear. The blank slides demonstrated cellular cross-contamination. So much for the veracity of the manufacturer's claim. Gary Gill -----Original Message----- From: John Auld [mailto:John.Auld@whnt.nhs.uk] Sent: Tuesday, January 20, 2004 12:21 PM To: histonet@lists.utsouthwestern.edu; vickroy.jim@mhsil.com Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: acceptable rate of carryover Jim I have a (very) vague recollection of a study done on carryover on an automated stainer for cervical cytology many many years ago. Don't remember what it said. BUT IMO there is only one acceptable rate for carryover and that is ZERO. This may not always be possible but that is what we should always work to. In my lab any slide with possible tissue carryover is always recut even if the suspect part is away from the section. regards John John Auld FIBMS MSc Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral Date: Tue, 20 Jan 2004 07:40:02 -0600 From: "Vickroy, Jim" Subject: [Histonet] carryover on slides To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <584AE8968B1EE14998210A0D6B97D854055AEE64@mmcmail2.mhsil.com> Content-Type: text/plain; charset="iso-8859-1" Has anyone ever seen any articles on what is an acceptable rate of carryover on routine slides? We have been monitoring our carryover rate and our pathologists wondered if anyone had ever seen a study on carryover rates. James R. Vickroy BS, HT (ASCP) Technical Supervisor, Surgical Pathology 788-4046 vickroy.jim@mhsil.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Tue Jan 20 11:31:28 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: Just jets for safety, for sure. Gary Gill -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Tuesday, January 20, 2004 12:20 PM To: Stapf, Ross Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Many many years ago - we started our first day in a newly built hospital histo lab just to realize that we had all the bench top jets we needed, i.e., air, gas, (we used a flame to embed with then) vacuum, water - but no one put in the 'guts' for any of those features - just the jets - for decoration, I suppose. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Stapf, Ross" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/20/2004 10:55 AM To: cc: Subject: RE: [Histonet] Re: Newly designed Labs At my old hospital when designing the lab somehow the blueprints were flipped and ventalation ducts ended up next to the cutting stations. Because the contractors wouldn't let us in the lab area until they were finished and our supervisor at the time didn't insist on inspecting the area, this wasn't noticed until they told us we could move in. Obviously they had to make a change before we could move in, costing money and time. Once construction starts inspect the area. Make sure you have authority to make changes after they start. It is likely that once things start to get in place you will see problems that are easier to correct in the middle of the renovation than after the renovation. Good luck. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From georgecole <@t> ev1.net Tue Jan 20 11:37:15 2004 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] The range of Histotech duities Message-ID: <000001c3df7c$0c5dabb0$044dbad0@hppav> Histotechs---- December 31st saw the end of my second year of retirement. And I am sending this note out on the Histonet of healthy, admiring and respectful discombobulation: You histotechs have leventy seven times the vocabulary that I had to learn when I started doing histoteching in 1962. I like very much to listen in on your communicatings back & forth from my rocking chair with my lap rug on my knees. But I have one complaint-----what in tarnation is this 83% of your communicating talking about? Your language keeps jumping out into the Great Beyond over Lillie's and Masson's hematoxylin, Bodian srong silver protein silver and all those dear old standbys that we all knew how to mix---they didn't work half the time---but we knew how to mix 'em! Why, when I opened the pages of my trusty 1933 Gray's Microtomists Formulary and Guide lately, a moth flew out in a gust of dust and fluttered into the light fixture, sighed and its poor little wings dissembled. You younguns don't seem to know it, but you are passing Medical Technicians on the inner track----passing them by with mentionables so crammed full of Science, you do to them what they did to us back then---snow 'em! All of the bibbity bobbity booing going out on the Histonet these days sounds so impressive, if I wore a hat, I would take it off to you all. Your anti and uncle bodies, immunos and moleclular mollycoddlin' look mighty good there coming out of the air on my combined Crystal Set AM radio and vacuumed tubed computater. Mind you, I'm not gojng to throw out my Ehrlich hematoxylin---some of the original--- mixed ought '86---19th C,.that is---. A body gets used to those blotchy green-gray-mud toned nuclei----and what a down-to-earth effect that is! So go ahead with your Greek mixin's----I'm for the Good Old Days-and what did we have in those Good Old Days? Nothing much, but we had the Good Old Days!!! Now how about puttin' your feet up on the stove railing next to the cracker barrel and let up for a spell on the Greek---there must be something else we can yarn about like---like---well, like muscle and nerve biopsies---- georgecole@ev1.net From info <@t> instrumedics.com Tue Jan 20 11:44:22 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] tissue carryover Message-ID: <014301c3df7d$0a1db150$6501a8c0@INSTRUMEDICS22> One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com From Janet.Bonner <@t> FLHOSP.ORG Tue Jan 20 11:56:39 2004 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Uranyl Nitrate Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F47@fh2k093.fhmis.net> We use 1% uranyl Nitrate aq from Poly Scientific (www.PolyRnD.com). -----Original Message----- From: james.zimmerman@pharma.novartis.com [mailto:james.zimmerman@pharma.novartis.com] Sent: Tuesday, January 20, 2004 11:33 AM To: Histonet@Pathology.swmed.edu Subject: [Histonet] Uranyl Nitrate Hello, Has anyone recently ordered uranyl nitrate? If so, could you please forward me the name and address of the vendor. Thanks, JPZ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From MAUGER <@t> email.chop.edu Tue Jan 20 11:57:20 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Pecam-1 (CD31) Message-ID: Hi Loralee, I have used a rat anti mouse CD31 from Pharmingen(BD Biosciences).Cat. #553370. I use a rabbit anti rat(mouse adsorbed)secondary-biotinylated,from Vector Labs. For retreival, use pepsin at 37C for 20-40 min. depending on fixation. I used the ABC detection from Vector with DAB. Good luck, Jo M >>> "Gehan, Loralee" 01/20/04 11:45AM >>> Has anyone out there come across a CD31 that works in formalin fixed paraffin embedded mouse tissue? Any retrieval methods used would help also. There are about 200 CD31's for human tissue. Can't seem to find one that works in mouse. Thanks a lot. Loralee Gehan Orthopaedics Research Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jan 20 12:11:07 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:27 2005 Subject: More on RE: [Histonet] carryover on slides In-Reply-To: Message-ID: <3.0.6.32.20040120111107.00bd2238@gemini.msu.montana.edu> Terry makes an excellent point here. If this is the type of carryover problem one is experiencing - some of these tricks may help. 1. Take a clean kimwipe (NOT kleenex tissues!), lay it flat on top of waterbath and skim this quickly across surface. Surface floaters of the larger variety aka carryover's will attach to kimwipe. Do this BETWEEN blocks or at least each case. A waterbath that is FULL to the brim with water is easier to skim! and, in my estimation, pick up a section. 2. Keep your BARE fingers out of the water to prevent exfoliating cells from skin out of water. These ugly little clingons show up on slides and on top of tissue sections. If your cells keep showing up in an excessive manner, wear gloves without powder. A good microtomist should be a clean freak and keep hands above the waves! 3. One lab I worked in insisted that tissue samples be staggered to have a cancer case? sandwiched between a noncancer case or some other tissue sample so the blocks were spaced away from each other during sectioning. This really didn't work all the time! What if all you had in one day's work was cancer cases!?? When I pointed out the fallacy of this method, they still insisted. At 03:00 PM 1/20/2004 -0000, you wrote: >Surely all are examples of carryover. 4. Keep your waterbath scrupulously clean, soap and hot water, whatever it takes. >Carryover is anything that "didn't ought to be there" and has come from another specimen. >It must be difficult to monitor, and the most difficult (i.e. impossible) is the like-to-like tissue carryover, that is to say, a bit of A's endometrium appearing on B's slide. >Cut-up and water bath are the most likely origins, and can be minimised by scrupulous cleanliness. > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: 20 January 2004 14:45 >To: Vickroy, Jim; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] carryover on slides > > >Could you define carryover? Do you mean cells, tissue fragments, stains >from one staining dish to another? > >At 07:40 AM 1/20/2004 -0600, you wrote: >>Has anyone ever seen any articles on what is an acceptable rate of carryover >>on routine slides? We have been monitoring our carryover rate and our >>pathologists wondered if anyone had ever seen a study on carryover rates. >> >> >>James R. Vickroy BS, HT (ASCP) >>Technical Supervisor, Surgical Pathology >>788-4046 >>vickroy.jim@mhsil.com >> >>This message (including any attachments) contains confidential information >>intended for a specific individual and purpose, and is protected by law. If >>you are not the intended recipient, you should delete this message. Any >>disclosure, copying, or distribution of this message, or the taking of any >>action based on it, is strictly prohibited. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From AFeatherstone <@t> KaleidaHealth.Org Tue Jan 20 12:41:03 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody Message-ID: Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From gcallis <@t> montana.edu Tue Jan 20 13:04:23 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Pecam-1 (CD31) In-Reply-To: <95774A6A6036D411AFEA00D0B73C8643088804BD@exmc3.urmc.roches ter.edu> Message-ID: <3.0.6.32.20040120120423.00bcfc10@gemini.msu.montana.edu> The antibody is available from BD Pharmingen and probably SEROTEC, but we only work with this marker on frozen sections. It is a monoclonal, rat antimouse. Check out the spec sheets from BD Pharmingen, they should tell you whether or not the antibody works on FFPE tissues. There is probably info already in Histonet archives, so do a search for whether the antibody works on FFPE, and retrieval method. At 11:45 AM 1/20/2004 -0500, you wrote: >Has anyone out there come across a CD31 that works in formalin fixed >paraffin embedded mouse tissue? Any retrieval methods used would help also. > >There are about 200 CD31's for human tissue. Can't seem to find one that >works in mouse. > >Thanks a lot. > > >Loralee Gehan >Orthopaedics Research Lab > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From carl.hobbs <@t> kcl.ac.uk Tue Jan 20 13:05:14 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Proteolytic enzyme....Dako replacement Message-ID: <002401c3df88$64d0fcf0$a91a8451@home> Be very grateful for any input.....Dako used to do a trypsin soln that was great for my BrdU disclosing immunostaining. They no longer provide this but now market a replacement, S 3007. This gives relatively poor disclosure over a range of incubation times eg 5-30mins. Additionally, the tissue, even at 10mins incubation( at room temp!), appears rather digested. ( did H&E on sequential section and that showed no evidence of proteolytic digestion)Thanks Carl From la.sebree <@t> hosp.wisc.edu Tue Jan 20 13:17:04 2004 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody Message-ID: Annette, We use C4d on frozen renal biopsies at 1:600 for 32" on Ventana automated staining instruments after 2" in Ventana's Morphosave. This is a DAB protocol. Our C4d is from Biogenesis, cat. # 2222-8004. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org] Sent: Tuesday, January 20, 2004 12:41 PM To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Jan 20 13:23:10 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FBF9@UTHEVS3.mail.uthouston.edu> A few comments It is some time since I helped to design a laboratory but I believe that the most important theme to keep in mind is flexibility. To this end the use of cantilever benches that allow cupboard units to be placed where you want is an ideal approach. This allows flexibility if the needs of the laboratory changes, as the type of cupboard units can be changed if the functions change. It also allows cupboard units to be removed if seating spaces are required. I think someone already mentioned the lack of electric outlets. Extra outlets should be available on walls even if there does not seem to be a need at this time. In addition to have some different electric outlets as regards 220 volts etc. Emergency outlets on a circuit fed by a separate generator would seem to be a must when tissues are being processed. Blocked in water also. It is much cheaper to have the basic electric and plumbing in place for future needs than to have new lines added at a later date. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Starkus, Laurie Sent: Tuesday, January 20, 2004 10:52 AM To: 'Cheasty, Sandra'; Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Sandra makes an excellent point here. I'm currently working in a Mohs lab that was just designed three years ago. Because no one thought to ask me (the only tech working here), no one considered that a cryostat generates a LOT of heat as it cools. Consequently, we are running a room air conditioner 24 hours a day, 7 days a week, 365 days a year. Yes, it was -10 outside this past week, and we are running an air conditioner. And, you still can't close the door or the air conditioner can't keep up. Also, they forgot to include room for the explosives cabinet (for ethanol) and our microscope is behind the door. So, when someone is using the microscope, the door isn't all the way open. Our surgeon sometimes gets hit in the butt with the door as people try to get into the lab. -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Tuesday, January 20, 2004 11:39 AM To: Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Frank, The biggest mistakes I've seen in designing labs or Information Systems is not including the end-user's input. Often the pathologists will have the last say, but they are not the ones who are processing the specimens from start to finish. You can avoid a lot of expensive changes after the fact, (and a lot of hurt feelings from staff) by having an avenue for staff to give input and keeping everyone aware of how things are proceeding. Whether the ideas are good and used, or not-so-good and discarded, I have found it worthwhile to include input from everyone. Also, if you have access to Microsoft Visio, (sort of a simple CAD software program) you can lay out the space easily and throw in counters and sinks and fume hoods and desks and re-arrange them in endless ways to give you ideas. Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 08:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Jan 20 13:29:25 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Pecam-1 (CD31) Message-ID: Novocastra NCL-CD31-1A10. Citrate 6.0pH HIER - works well on formalin fixed murine tissue. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics Angela McNabola Sent by: histonet-bounces@lists.utsouthwestern.edu 01/20/2004 11:07 AM To: Loralee_Gehan@URMC.Rochester.edu, histonet@pathology.swmed.edu cc: Subject: Re: [Histonet] Pecam-1 (CD31) Hi, We use Santa Cruz PECAM-1 M-20 (SC-15060) goat polyclonal. We steam for 30 minutes in Dako TRS (S1699) for 30 minutes (cool to room temperature). We also avidin/biotin block (Vector), and use ABC (Vector elite kit). Works great, especially on mouse xenografts. Let me know if you need more information. Angela McNabola, MS, HT(ASCP)SLS Bayer Corp. West Haven, CT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caroline.stott <@t> anatomy.otago.ac.nz Tue Jan 20 13:43:40 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] glycol methacrylate and sectioning Message-ID: <5.2.1.1.0.20040121084016.024636c0@anatomy.otago.ac.nz> Yes I am currently cutting 30 um sections with Glycol Methacrylate. We have done 40 um also. We haven't had a problem, provided the block is moist. Give it a try, the results are very good. And staining isn't a problem either. Caroline Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From nick.kirk3 <@t> btopenworld.com Tue Jan 20 13:40:55 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody In-Reply-To: Message-ID: We use Novocastra's CD4 on FFPE sections using Vector's Antigen Unmasking Fluid in a pressure cooker and Dako's ChemMate HRP detection kit which uses DAB. We use our routine method and have few problems getting it to work. The biggest problem is the availability of good quality tonsil control material, that hasn't been fixing for years in the cupboard. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Featherstone, Annette Sent: 20 January 2004 18:41 To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nick.kirk3 <@t> btopenworld.com Tue Jan 20 13:40:55 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:27 2005 Subject: More on RE: [Histonet] carryover on slides In-Reply-To: <3.0.6.32.20040120111107.00bd2238@gemini.msu.montana.edu> Message-ID: Another possible source of "carryover" is from the cut-up board. I have seen cases where tissue is present in the block which has been picked up from the cut-up board as well as the more conventional reasons such as a dirty waterbath or "floaters" in the staining machine. In my experience some Pathologists are more conscious of this than others and there is a varying degree of "cleanliness" depending on the person doing the grossing. Until recently we had to use the same staining machine for both our routine H&Es and PAP stains. On one occasion last year we had a nice fragment of cervical microbiopsy from a cervical smear present itself on an appendix case. Either the surgeon got it horribly wrong or one of the reagents on the staining machine had "strays" in it. We now have separate staining machines so the "strays" shouldn't be a problem anymore (I hope), but it does raise interesting questions especially where batch staining of cervical smears takes place. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: 20 January 2004 18:11 To: Marshall Terry Dr,Consultant Histopathologist; Histonet@lists.utsouthwestern.edu Subject: More on RE: [Histonet] carryover on slides Terry makes an excellent point here. If this is the type of carryover problem one is experiencing - some of these tricks may help. 1. Take a clean kimwipe (NOT kleenex tissues!), lay it flat on top of waterbath and skim this quickly across surface. Surface floaters of the larger variety aka carryover's will attach to kimwipe. Do this BETWEEN blocks or at least each case. A waterbath that is FULL to the brim with water is easier to skim! and, in my estimation, pick up a section. 2. Keep your BARE fingers out of the water to prevent exfoliating cells from skin out of water. These ugly little clingons show up on slides and on top of tissue sections. If your cells keep showing up in an excessive manner, wear gloves without powder. A good microtomist should be a clean freak and keep hands above the waves! 3. One lab I worked in insisted that tissue samples be staggered to have a cancer case? sandwiched between a noncancer case or some other tissue sample so the blocks were spaced away from each other during sectioning. This really didn't work all the time! What if all you had in one day's work was cancer cases!?? When I pointed out the fallacy of this method, they still insisted. At 03:00 PM 1/20/2004 -0000, you wrote: >Surely all are examples of carryover. 4. Keep your waterbath scrupulously clean, soap and hot water, whatever it takes. >Carryover is anything that "didn't ought to be there" and has come from another specimen. >It must be difficult to monitor, and the most difficult (i.e. impossible) is the like-to-like tissue carryover, that is to say, a bit of A's endometrium appearing on B's slide. >Cut-up and water bath are the most likely origins, and can be minimised by scrupulous cleanliness. > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: 20 January 2004 14:45 >To: Vickroy, Jim; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] carryover on slides > > >Could you define carryover? Do you mean cells, tissue fragments, stains >from one staining dish to another? > >At 07:40 AM 1/20/2004 -0600, you wrote: >>Has anyone ever seen any articles on what is an acceptable rate of carryover >>on routine slides? We have been monitoring our carryover rate and our >>pathologists wondered if anyone had ever seen a study on carryover rates. >> >> >>James R. Vickroy BS, HT (ASCP) >>Technical Supervisor, Surgical Pathology >>788-4046 >>vickroy.jim@mhsil.com >> >>This message (including any attachments) contains confidential information >>intended for a specific individual and purpose, and is protected by law. If >>you are not the intended recipient, you should delete this message. Any >>disclosure, copying, or distribution of this message, or the taking of any >>action based on it, is strictly prohibited. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nick.kirk3 <@t> btopenworld.com Tue Jan 20 13:43:20 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody In-Reply-To: Message-ID: Oops, must need my eyes testing, just read this again and noticed it is an enquiry for C4d not CD4. Ignore the previous mumblings of an addled person. Nick Kirk -----Original Message----- From: Nick Kirk [mailto:nick.kirk3@btopenworld.com] Sent: 20 January 2004 19:41 To: Histonet Subject: RE: [Histonet] C4d antibody We use Novocastra's CD4 on FFPE sections using Vector's Antigen Unmasking Fluid in a pressure cooker and Dako's ChemMate HRP detection kit which uses DAB. We use our routine method and have few problems getting it to work. The biggest problem is the availability of good quality tonsil control material, that hasn't been fixing for years in the cupboard. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Featherstone, Annette Sent: 20 January 2004 18:41 To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Tue Jan 20 13:29:45 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody Message-ID: We also use the Biogenisis C4d for Frozen. For Paraffin sections we use Alpco Diagnostics CAT #004-BI-RC4D (www.alpco.com) at 1:40. Pretreatment is citrate buffer (pH 6.0) in the pressure cooker for 10 minutes. We use the Envision + detection system from Dako. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, January 20, 2004 1:17 PM To: Featherstone, Annette; Instrumedics; HistoNet Server Subject: RE: [Histonet] C4d antibody Annette, We use C4d on frozen renal biopsies at 1:600 for 32" on Ventana automated staining instruments after 2" in Ventana's Morphosave. This is a DAB protocol. Our C4d is from Biogenesis, cat. # 2222-8004. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org] Sent: Tuesday, January 20, 2004 12:41 PM To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Tue Jan 20 13:53:27 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody Message-ID: I made the same mistake for the first month I was here. You should have seen the look on my immuno techs' faces when after they asked me to find a new source for C4D, I handed them a whole bunch of CD4 spec sheets. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nick Kirk Sent: Tuesday, January 20, 2004 1:43 PM To: Histonet Subject: RE: [Histonet] C4d antibody Oops, must need my eyes testing, just read this again and noticed it is an enquiry for C4d not CD4. Ignore the previous mumblings of an addled person. Nick Kirk -----Original Message----- From: Nick Kirk [mailto:nick.kirk3@btopenworld.com] Sent: 20 January 2004 19:41 To: Histonet Subject: RE: [Histonet] C4d antibody We use Novocastra's CD4 on FFPE sections using Vector's Antigen Unmasking Fluid in a pressure cooker and Dako's ChemMate HRP detection kit which uses DAB. We use our routine method and have few problems getting it to work. The biggest problem is the availability of good quality tonsil control material, that hasn't been fixing for years in the cupboard. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Featherstone, Annette Sent: 20 January 2004 18:41 To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nick.kirk3 <@t> btopenworld.com Tue Jan 20 14:03:21 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody In-Reply-To: Message-ID: I blame it on all those years of inhaling xylene fumes before the phrase "Health and Safety" was invented. Demyelinatedly yours Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: Stapf, Ross [mailto:RossS@BaylorHealth.edu] Sent: 20 January 2004 19:53 To: Nick Kirk; Histonet Subject: RE: [Histonet] C4d antibody I made the same mistake for the first month I was here. You should have seen the look on my immuno techs' faces when after they asked me to find a new source for C4D, I handed them a whole bunch of CD4 spec sheets. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nick Kirk Sent: Tuesday, January 20, 2004 1:43 PM To: Histonet Subject: RE: [Histonet] C4d antibody Oops, must need my eyes testing, just read this again and noticed it is an enquiry for C4d not CD4. Ignore the previous mumblings of an addled person. Nick Kirk -----Original Message----- From: Nick Kirk [mailto:nick.kirk3@btopenworld.com] Sent: 20 January 2004 19:41 To: Histonet Subject: RE: [Histonet] C4d antibody We use Novocastra's CD4 on FFPE sections using Vector's Antigen Unmasking Fluid in a pressure cooker and Dako's ChemMate HRP detection kit which uses DAB. We use our routine method and have few problems getting it to work. The biggest problem is the availability of good quality tonsil control material, that hasn't been fixing for years in the cupboard. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Featherstone, Annette Sent: 20 January 2004 18:41 To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From browning <@t> HHSC.CA Tue Jan 20 15:04:51 2004 From: browning <@t> HHSC.CA (Browning Deb) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] C4d antibody Message-ID: <3AADFB88753AD31189C100902786B91C0E277F9A@hch_nt_exchange.hhsc.ca> We are experiencing the same thing. We originally tried the antibody from Biomedica (distributed by Medicorp in Montreal), with no success, and now are about to try IF. However, a colleague in Toronto is successful with the Quidel Corporation antibody, A213, (distributed by Cedarlane) on frozen sections, using the Ventana Benchmark, avidin/biotin system. Despite all claims, I haven't found anyone yet who actually has C4d working on PPFE, just on frozen sections. Please share your success stories!!! -----Original Message----- From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org] Sent: Tuesday, January 20, 2004 1:41 PM To: 'Instrumedics'; HistoNet Server Subject: [Histonet] C4d antibody Hi Anyone out there using C4d antibody? Have to work it up and want to know if there are any tips to make this work as they haven't been able to get it to work right. Manufacturer recommends Alk Phos, but I was hoping to get it to work with DAB. Any suggestions? Thanks Annette Featherstone HT/MLT -----Original Message----- From: Instrumedics [mailto:info@instrumedics.com] Sent: Tuesday, January 20, 2004 12:44 To: HistoNet Server Subject: [Histonet] tissue carryover One of the advantages of the Cryo-Vac-Away vacuum system for the cryostat is that the debris generated during the trimming step is suctioned away as it is generated at the blockface. This substantially reduces the chances that any sections will cling to the underside of the knife which can then can carryover to the following case. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From BRobert <@t> ameripath.com Tue Jan 20 16:19:37 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs Message-ID: These are very good observations, I experienced similar problems in the past with lab not designed to accomodate the equipment size, proximity to water for stainers for example, cabinetery too low to be able to fit any equipment on counters etc. the ventilation is definitely something to be planned as you do not want any fumes in the rooms but not too much air draft in the cutting area either but good fresh air where you have heat producing equipment. Including the techs in the planning is a must if you do not want to remodel your lab all the time once it's done! Good luck! p.s. I remember a workshop on the subject at the NSH meeting a few years ago, maybe someone has more details or a copy of the hand out for you! Brigitte -----Original Message----- From: Starkus, Laurie [mailto:StarkusL@ummhc.org] Sent: Tuesday, January 20, 2004 8:52 AM To: 'Cheasty, Sandra'; Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Sandra makes an excellent point here. I'm currently working in a Mohs lab that was just designed three years ago. Because no one thought to ask me (the only tech working here), no one considered that a cryostat generates a LOT of heat as it cools. Consequently, we are running a room air conditioner 24 hours a day, 7 days a week, 365 days a year. Yes, it was -10 outside this past week, and we are running an air conditioner. And, you still can't close the door or the air conditioner can't keep up. Also, they forgot to include room for the explosives cabinet (for ethanol) and our microscope is behind the door. So, when someone is using the microscope, the door isn't all the way open. Our surgeon sometimes gets hit in the butt with the door as people try to get into the lab. -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Tuesday, January 20, 2004 11:39 AM To: Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Frank, The biggest mistakes I've seen in designing labs or Information Systems is not including the end-user's input. Often the pathologists will have the last say, but they are not the ones who are processing the specimens from start to finish. You can avoid a lot of expensive changes after the fact, (and a lot of hurt feelings from staff) by having an avenue for staff to give input and keeping everyone aware of how things are proceeding. Whether the ideas are good and used, or not-so-good and discarded, I have found it worthwhile to include input from everyone. Also, if you have access to Microsoft Visio, (sort of a simple CAD software program) you can lay out the space easily and throw in counters and sinks and fume hoods and desks and re-arrange them in endless ways to give you ideas. Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 08:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bills <@t> icpmr.wsahs.nsw.gov.au Tue Jan 20 16:47:17 2004 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs In-Reply-To: Message-ID: <000001c3dfa7$5b5d27b0$3187080a@wsahs.nsw.gov.au> All these are good comments, but don't forget the people (numbers) who might work in these areas as a human body gives off heat as well as equipment. Most airconditioning is rated against human heat production and not the extra heat of any equipment and the combined load is quite often large. I cannot remember the exact figure but I think the human rating is equivalent to a 500w element. Easily height adjusted benches are also essential to account for varying stature of individuals. Particularly in microtomy. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of BRobert@ameripath.com Sent: Wednesday, 21 January 2004 9:20 AM To: StarkusL@ummhc.org; SCheasty@ahs.llumc.edu; jlinda@ces.clemson.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs These are very good observations, I experienced similar problems in the past with lab not designed to accomodate the equipment size, proximity to water for stainers for example, cabinetery too low to be able to fit any equipment on counters etc. the ventilation is definitely something to be planned as you do not want any fumes in the rooms but not too much air draft in the cutting area either but good fresh air where you have heat producing equipment. Including the techs in the planning is a must if you do not want to remodel your lab all the time once it's done! Good luck! p.s. I remember a workshop on the subject at the NSH meeting a few years ago, maybe someone has more details or a copy of the hand out for you! Brigitte -----Original Message----- From: Starkus, Laurie [mailto:StarkusL@ummhc.org] Sent: Tuesday, January 20, 2004 8:52 AM To: 'Cheasty, Sandra'; Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Sandra makes an excellent point here. I'm currently working in a Mohs lab that was just designed three years ago. Because no one thought to ask me (the only tech working here), no one considered that a cryostat generates a LOT of heat as it cools. Consequently, we are running a room air conditioner 24 hours a day, 7 days a week, 365 days a year. Yes, it was -10 outside this past week, and we are running an air conditioner. And, you still can't close the door or the air conditioner can't keep up. Also, they forgot to include room for the explosives cabinet (for ethanol) and our microscope is behind the door. So, when someone is using the microscope, the door isn't all the way open. Our surgeon sometimes gets hit in the butt with the door as people try to get into the lab. -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Tuesday, January 20, 2004 11:39 AM To: Linda Jenkins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Newly designed Labs Frank, The biggest mistakes I've seen in designing labs or Information Systems is not including the end-user's input. Often the pathologists will have the last say, but they are not the ones who are processing the specimens from start to finish. You can avoid a lot of expensive changes after the fact, (and a lot of hurt feelings from staff) by having an avenue for staff to give input and keeping everyone aware of how things are proceeding. Whether the ideas are good and used, or not-so-good and discarded, I have found it worthwhile to include input from everyone. Also, if you have access to Microsoft Visio, (sort of a simple CAD software program) you can lay out the space easily and throw in counters and sinks and fume hoods and desks and re-arrange them in endless ways to give you ideas. Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Tuesday, January 20, 2004 08:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Newly designed Labs Hi, Frank, Based on my previous experience with designing a new lab, the one thing we didn't check on was the location of the critical power outlets. The electrical people put in the correct number BUT they put them all on one wall. Also, we needed at least one - two more sinks. Have fun planning! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. 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From BRobert <@t> ameripath.com Tue Jan 20 16:54:34 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Salary adjustments Message-ID: We are encouraging people in the lab to get their HT certification, now we are discussing how we should re-adjust the salaries of those who get certified. We want it to be significant enough to really encourage people to go for it and value the effort, but also want a good balance. I would like to know what other labs are doing. Is that included to the annual review as raise justification? Do you have a specific salary adjustment given automatically when they receive their certificate? What is the average value $$ do you give to the certification? Any feedback on that would be appreciated. Thanks! Brigitte Robert Histology Laboratory Manager APMG Inc. 408-399-5050 ext 106 fax: 408-354-4581 From ploykasek <@t> phenopath.com Tue Jan 20 17:14:17 2004 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Re: Newly designed Labs In-Reply-To: <566FB0B522443D43AF02D2ADBE35A6F077FBF9@UTHEVS3.mail.uthouston.edu> Message-ID: We moved into a new lab about a year ago. It's great. You should see the views we have - we're on the ship canal in Seattle. Lovely. But, back to ideas. Not only do you need enough electrical outlets, but be sure that you have enough circuits. We have a lot of microwaves, steamers,ect... Going at once & we have blown circuits. We have adjustable lab benches ? the height adjusts & the shelving can change. I really like this feature, very adaptable. One thing we added at the end of one bench was a series of cubby holes that hold slide pallets to organize everyone?s projects. I?ll try to think of more ideas. Sorry, I can?t ship you the view. Patti Loykasek PhenoPath Laboratories Seattle, WA> A few comments > It is some time since I helped to design a laboratory but I believe that > the most important theme to keep in mind is flexibility. To this end the > use of cantilever benches that allow cupboard units to be placed where > you want is an ideal approach. This allows flexibility if the needs of > the laboratory changes, as the type of cupboard units can be changed if > the functions change. It also allows cupboard units to be removed if > seating spaces are required. > I think someone already mentioned the lack of electric outlets. Extra > outlets should be available on walls even if there does not seem to be a > need at this time. In addition to have some different electric outlets > as regards 220 volts etc. > Emergency outlets on a circuit fed by a separate generator would seem to > be a must when tissues are being processed. > Blocked in water also. It is much cheaper to have the basic electric and > plumbing in place for future needs than to have new lines added at a > later date. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Starkus, > Laurie > Sent: Tuesday, January 20, 2004 10:52 AM > To: 'Cheasty, Sandra'; Linda Jenkins; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Newly designed Labs > > > Sandra makes an excellent point here. I'm currently working in a Mohs > lab that was just designed three years ago. Because no one thought to > ask me (the only tech working here), no one considered that a cryostat > generates a LOT of heat as it cools. Consequently, we are running a > room air conditioner 24 hours a day, 7 days a week, 365 days a year. > Yes, it was -10 outside this past week, and we are running an air > conditioner. And, you still can't close the door or the air conditioner > can't keep up. > > Also, they forgot to include room for the explosives cabinet (for > ethanol) and our microscope is behind the door. So, when someone is > using the microscope, the door isn't all the way open. Our surgeon > sometimes gets hit in the butt with the door as people try to get into > the lab. > > -----Original Message----- > From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] > Sent: Tuesday, January 20, 2004 11:39 AM > To: Linda Jenkins; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Newly designed Labs > > > Frank, > The biggest mistakes I've seen in designing labs or Information > Systems is not including the end-user's input. Often the pathologists > will have the last say, but they are not the ones who are processing the > specimens from start to finish. You can avoid a lot of expensive changes > after the fact, (and a lot of hurt feelings from staff) by having an > avenue for staff to give input and keeping everyone aware of how things > are proceeding. Whether the ideas are good and used, or not-so-good and > discarded, I have found it worthwhile to include input from everyone. > Also, if you have access to Microsoft Visio, (sort of a simple > CAD software program) you can lay out the space easily and throw in > counters and sinks and fume hoods and desks and re-arrange them in > endless ways to give you ideas. > > Sandy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda > Jenkins > Sent: Tuesday, January 20, 2004 08:25 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Newly designed Labs > > Hi, Frank, > Based on my previous experience with designing a new lab, the > one > thing we didn't check on was the location of the critical power > outlets. The electrical people put in the correct number BUT they put > them > all on one wall. Also, we needed at least one - two more sinks. > Have fun planning! > Linda > > Linda Jenkins, HT > Clemson University > Dept. of Bioengineering > Clemson, SC 29634-0905 > 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains > information that may be confidential, protected by applicable legal > privileges, or constitute non-public information. It is intended to be > conveyed only to the designated recipient(s). If you are not an intended > recipient of this message, please notify the sender by replying to this > message and then delete it from your system. Use, dissemination, > distribution or reproduction of this message by unintended recipients is > not authorized and may be unlawful. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegg <@t> colobio.com Tue Jan 20 17:16:42 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Quantitative von Kossa In-Reply-To: <200401201532.i0KFWeAW092584@smtp.ufl.edu> Message-ID: Brian, never did this on cells but used to use VK to measure calcified area in bone biopsies allt the time. We used an image analysis system with a video camera on a scope and would measure the total area in a field (usually 10x) and then measure the black calcified area which got reported out as "total area of sample calcified". not sure how you could do this on cells. with bone we had standards for different components and they stayed in place in relation to each other. we knew given a sample size how much of it should be cortical bone, trabecular bone, marrow, fat, etc. i suppose if you know the normal area of your cells when they are not calcified you could apply something like this. I would refer you to a paper we wrote An Indirect Method of Measuring Widths Suitable for Automated Bone Histomorphometry Journal of Bone and Mineral Research Vol. 7 NO. 12, 1992 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brian Hatcher Sent: Tuesday, January 20, 2004 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quantitative von Kossa Is anyone aware of a technique/references on extracting quantitative information from a von Kossa stain. I am interested in trying to measure the rate and extent of mineralization in a marrow stromal cell culture, and would like to know if this is possible using this stain, and also what target values I should be trying to achieve. Thanks. Brian Hatcher Graduate Research Asst. Dept. of Biomedical Engineering University of Florida _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Tue Jan 20 17:19:22 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] antibody for bone alkaline phosphatase In-Reply-To: <1509D822.767EFC64.0005167B@aol.com> Message-ID: Haven't used an antibody but have used histochemical method to demonstrat alkaline phosphatase in osteoblasts on GMA embedded undecalcified bone fixed in cold methanol. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Myri37@aol.com Sent: Tuesday, January 20, 2004 7:58 AM To: histonet@pathology.swmed.edu Subject: [Histonet] antibody for bone alkaline phosphatase Hi everyone does anyone know a reference of bone alkaline phosphatase antibody can we use it on paraffin or MMA embedded samples ? thank you very much for your help myriam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jan 20 17:27:47 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] More on "Newly designed Labs", very blabby wish list In-Reply-To: <000001c3dfa7$5b5d27b0$3187080a@wsahs.nsw.gov.au> References: Message-ID: <3.0.6.32.20040120162747.00bd5f68@gemini.msu.montana.edu> Having moved into two new laboratory spaces within one year (one thought out cleverly and designed with histology work in mind, and the other - a willy nilly, make it work space!), the lighting is absolutely critical when performing microtomy, embedding, grossing in tissues, and immunostaining (if one does manual staining). Make sure the lights are placed above lab benches and other work areas - these should not be blocked by upper shelves. The constant moving of lights to optimize lighting for microtomy, embedding and pipetting immunoreagents onto sections is a pain. Consider how the work flows during the day: 1. Processing followed by 2. embedding followed by 3. microtomy followed by 4. staining, etc, etc. A joy to not be running into someone all the time. Consider a special area for immunostaining and its prepartion time/protocols, the same for grossing in tissues. I find a standard lower bench height often too high for microtomy, when my arms are in upward slant rather than relaxed and laying flat on the bench top. It often is important to look down at top edge of block for alignment and fine adjustments. How about a built in paraffin trimming disposal "tray" aka drawer just in front of the microtome? Pull it out and clean quickly, and made of something waterproof?? Plug in outlets should be designed to not interfer with the backside of ANY instrument and must be in easy reach or drill holes in benchtops to access outlets easilty at underbench, lower level. I get tired of moving heavy instruments or standing on my head along with crawling on hands and knees to plug/unplug things! Consider L formation for microtomy area, with microtome in front of you, turn slightly to lay ribbon on a waterbath on L shaped modular benchtop. This modular L should have drawers built in - preferably on wheels that lock down to not sacrifice other storage space and aid floor cleaning. The module would have capability for placement on opposite side for those who are lefthanded or trained to work that direction. Be sure to buy ergonomic chairs for your techs, with pneumatic height adjustments. My dream lab would have my two cryostats next to higher lab benches (on either side of instrument, but not interfering with flywheels nor cooling vents). It is nice to set things within arms reach to the side rather than reach up and over a cryostat chamber. This works if your cryostat is in the new lab. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From SCheasty <@t> ahs.llumc.edu Tue Jan 20 17:47:00 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Histology Part-Timers? Message-ID: <2E50F33F91EEDA46A77BC3B2575BB09105873D@mars.llumc.edu> There is an opening for a part-time histologist in the San Bernardino area of southern California. * 6:30-10:30 M-F * Plenty of time left in the day to get to the beach, spot celebrities, etc. * Well established lab, routine histology * Numerous pot lucks * Friendly co-workers Please e-mail me if you are interested.... Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From Histolady710 <@t> aol.com Tue Jan 20 18:13:26 2004 From: Histolady710 <@t> aol.com (Histolady710@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] HT Certified vs. HT non-certified Message-ID: <1a0.1f86bad9.2d3f1e26@aol.com> This is for histology managers ! I'm curious as to which HT you would hire - a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years experience. Thanks. From peptolab <@t> hamptons.com Tue Jan 20 18:47:07 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Grossing Pay- Saturdays Message-ID: <001401c3dfb8$1ac5c110$95a5bd18@JEFF> We have a per diem tech who comes in Saturdays and embeds, cuts, stains, and coverslips our entire Friday workload- from 40 to 100 blocks per day. This is nice since it gives my tech and a half a day to catch up on chores with virtually no histo other than specials or recuts- the pathologists like it too since they can read at the time they arrive. As for grossing- if someone is just pouring endoscopic biosies and curettings into cassettes- I don't think a pay differential is indicated. Appendices, gall bladders, simple skin lesions and especially placentas in the mix deserves a moderate salary differential. However, if , after conferring with the pathologist, they are grossing colectomies, evaluating margins in skin, breast, or soft tissue resections, or staging radical surgeries for colon, breast, stomach, kidney, bladder, prostate, uterine and lung cancers with lymph node searches, then a substantial differential is indicated along the lines of a PA's pay, especially if 50% or more of their time is spent doing these "complex testing" duties. Of course, I am prejudiced. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant/Histology Supervisor/Immunohistochemist/Histotech/Specimen Courrier and Schlepper in chief/Laboratory Safety Officer Southside Hospital Bay Shore, NY From BRobert <@t> ameripath.com Tue Jan 20 18:42:42 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] HT Certified vs. HT non-certified Message-ID: It is not all that black or white! It all depends on the person and what we are looking for...each case is unique and has to be considered and evaluated in it's context. I would certainly never base a decision only on the #years of experience and certified or not certified! BR -----Original Message----- From: Histolady710@aol.com [mailto:Histolady710@aol.com] Sent: Tuesday, January 20, 2004 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Certified vs. HT non-certified This is for histology managers ! I'm curious as to which HT you would hire - a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years experience. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccdub <@t> earthlink.net Tue Jan 20 19:37:21 2004 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] CBG vs. B & R Message-ID: We are currently demo-ing a CBG recycling unit. We are using it for Xylene and Formalin only. B & R found out about our search for a recycler and has contacted us. Other than being very pushy, their unit looks comparable to the CBG. The only plus I see in their unit is that you can run cycles for both reagents at the same time. I am curious as to what others are using and the volumes they are running. Any feedback both pros and cons of both these instruments would be greatly appreciated. Cindy DuBois Delta Pathology Stockton, CA From Mplhisto <@t> aol.com Tue Jan 20 20:41:38 2004 From: Mplhisto <@t> aol.com (Mplhisto@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: <19c.1f3f894e.2d3f40e2@aol.com> Our lab will be looking for an automatic Special/ Immuno stainer this year. I was curious to see which stainers you would recommend. We do a large volume of Immunos and specials and we are currently using the Cytologix. Any feedback positive or negative would be greatly appreciated. Thanks, Meredith Hale HT ( ASCP) From CrochiereSteve <@t> aol.com Tue Jan 20 20:40:22 2004 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] CBG vs. B & R Message-ID: <10d.2f03aa0d.2d3f4096@aol.com> Cindy, I have used BR in the past and know of a lab that uses their machine. I currently have 2 CBG machines, one for xylene & Histosolve and the second for alcohol only. I think the CBG machines are great. I have been using them every day for 3 years (since I joined my present group) and have never had a problem that required service. The machines were in use prior to my arrival, are at least 6-10 years old and the customer service rep call at least twice a year to make sure they're still running ok. I think that's amazing these days. Steven M. Crochiere, HT (ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center 299 Carew St. Springfield, MA 01104 From DMBCMP <@t> aol.com Tue Jan 20 21:23:43 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] To Joe Nocito...CLIA Regs Message-ID: <8a.1a0fdf1.2d3f4abf@aol.com> Hello, Joe: My Lab manager is very interested in your information about 'person's other than Pathologists doing gross descriptions". Is there a specific CLIA reg you can quote for us or someplace where we can get specifics on the "prior to 1995" training? Do you know if state regs would overide the CLIA reg? This is very important to our lab. I am glad I saw it discussed here on Histonet. Your help will be very much appreciated. Thank you. Dannie Blake HT(ASCP) Histo Lead Tech Fresno Community Hospital Fresno, California From slakmon <@t> hotmail.com Tue Jan 20 21:28:35 2004 From: slakmon <@t> hotmail.com (Philip Slakmon) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] (no subject) Message-ID: I Philip Slakmon, would like slakmon@hotmail.com to be added to your list. Thank you ! From DMBCMP <@t> aol.com Tue Jan 20 21:37:12 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin Message-ID: Hi, everyone: In order to settle a difference of opinion between generations of trained Histotechs in my lab, may I have feedback from anyone interested in responding? Do you cover the blocks in melted paraffin in the embedding centre reservior and hold them that way while embedding? or.... Do you dump them in the warm reservior "dry" (not covered in melted paraffin) and embed them that way? Do you consider this "dry" method as bad technique since a tiny biopsy specimen MAY not be noticed as the paraffin quickly solidifies? We have a dispute. I have researched every book I can find and there is no reference to it anywhere. A newly trained tech that came to work for us said no mention was made during her training period. Some techs did...and some didn't. As a tech of nearly 27 years, I find this practice to be just asking for trouble. I was trained to keep everything melted. There seems to be some argument against keeping the cassettes in the melted paraffin, claiming it "cooks" the biopsies. I don't buy it, but what are the opinions of others? It seems so basic to me. I hope this does not come across as frivilous. Thanks for your input. Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Medical Centre Fresno, California From DDDeltour <@t> sig.med.navy.mil Wed Jan 21 00:05:13 2004 From: DDDeltour <@t> sig.med.navy.mil (Deltour, Douglas D.(HM2)) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] HT Certified vs. HT non-certified Message-ID: I think they you have to look more at experience than certification. When you think about it what does the certification actually mean? They studied for a test and passed it. They did a couple of stains or had someone do it and they were acceptable. Does everyone actually know the concepts behind ALL of the certification questions or do they just know the answers? When will they apply those questions to the work? I have worked with both certified and non-certified techs. By watching them work you could not distinguish which ones were certified and which ones were not. I am not trying to knock certification but some facilities may pass over good techs or lose good techs because of it. Look at the big picture. Douglas D. Deltour Naval Hospital Sigonella Italy Supervisor Histology Histology Technician -----Original Message----- From: BRobert@ameripath.com [mailto:BRobert@ameripath.com] Sent: Wednesday, January 21, 2004 1:43 AM To: Histolady710@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT Certified vs. HT non-certified It is not all that black or white! It all depends on the person and what we are looking for...each case is unique and has to be considered and evaluated in it's context. I would certainly never base a decision only on the #years of experience and certified or not certified! BR -----Original Message----- From: Histolady710@aol.com [mailto:Histolady710@aol.com] Sent: Tuesday, January 20, 2004 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Certified vs. HT non-certified This is for histology managers ! I'm curious as to which HT you would hire - a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years experience. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This document may contain information covered under the privacy Act, 5 USC 552(a), and/or the Health Insurance Portability and Accountability Act (PL 104-191) and its various implementing regulations and must be protected in accordance with those provisions. Healthcare information is personal and sensitive and must be treated accordingly. If this correspondence contains healthcare information it is being provided to you after applying the appropriate security controls and authorization from the patient, or under circumstances that don't require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Redisclosure without additional patient consent or as permitted by law is prohibited. Unauthorized redisclosure or failure to maintain confidentiality subjects you to application of appropriate sanction. If you have received this correspondence in error, please notify the sender and the command Privacy Officer at privacy@sig.med.navy.mil at once and destroy any copies you have made. From nick.kirk3 <@t> btopenworld.com Wed Jan 21 00:20:22 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin In-Reply-To: Message-ID: Dannie I agree with you entirely. The argument that the biopsies will "cook" is totally spurious, after all, they've been sitting in molten paraffin wax on the processing machine for several hours before reaching the embedding stage so a few more minutes will have no effect. Also speaking from personal experience, embedding a "wet" piece of tissue is much easier than embedding a "warm but dry" piece of tissue. This is especially useful when you have a small piece of tissue requiring a particular orientation. The point you make about small biopsies is also a good one. You might be able to get away with using the "warm but dry" technique if you only had a few blocks to embed, but any lab with a sizeable through put should avoid that method of embedding in my opinion (for what it's worth). I think I'm probably correct in saying that here in the UK, the "wet" method is used in almost every lab in the country, if not all of the labs in the country. Nick Kirk Head Biomedical Scientist Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DMBCMP@aol.com Sent: 21 January 2004 03:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding W/WO Melted Paraffin Hi, everyone: In order to settle a difference of opinion between generations of trained Histotechs in my lab, may I have feedback from anyone interested in responding? Do you cover the blocks in melted paraffin in the embedding centre reservior and hold them that way while embedding? or.... Do you dump them in the warm reservior "dry" (not covered in melted paraffin) and embed them that way? Do you consider this "dry" method as bad technique since a tiny biopsy specimen MAY not be noticed as the paraffin quickly solidifies? We have a dispute. I have researched every book I can find and there is no reference to it anywhere. A newly trained tech that came to work for us said no mention was made during her training period. Some techs did...and some didn't. As a tech of nearly 27 years, I find this practice to be just asking for trouble. I was trained to keep everything melted. There seems to be some argument against keeping the cassettes in the melted paraffin, claiming it "cooks" the biopsies. I don't buy it, but what are the opinions of others? It seems so basic to me. I hope this does not come across as frivilous. Thanks for your input. Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Medical Centre Fresno, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Hedley.Glencross <@t> CMMC.nhs.uk Wed Jan 21 02:22:28 2004 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] carry over Message-ID: Hi everyone I can confirm Nick's scenario, when I was a histology TEQA facilitator, the very first H&E's looked at had some Pap stained cervical cells over the section. Also, another good source of carry over is the embedding station. If anyone has ever cleaned out wax filled forcep warming blocks (like those on the old Shandon embedder), they will have discovered a treasure trove of tissue pieces! On the cytology front, it is very good practice to split gynae & non gynae staining, but also the quality of the non gynae preps can help, by achieving mono layers rather than multilayers. Remember cells stick really well to glass, but very poorly to each other. I wonder if LBC will help? However, if you do get carry over of cervical cells from one smear to another during batch staining, it may be almost impossible to tell. A possible source of false positive cytology and consequent over investigation that is poorly recognised. Good technique is essential. Regards Hedley Glencross Manchester Cytology Centre UK From alex.knisely <@t> kcl.ac.uk Wed Jan 21 05:31:57 2004 From: alex.knisely <@t> kcl.ac.uk (Alex Knisely) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] CD13 -- FFPE immunohistochemistry Message-ID: <3.0.6.32.20040121113157.0114b2e0@137.73.66.5> CD13, also called "aminopeptidase N", is a white-cell antigen usually assessed by flow cytometry. I'd like to look at it in FFPE materials. A search through ABCAM has not found a reference to a commercially available source of an anti-CD13 antibody that works in FFPE sections. Can anyone recommend a source and protocol for such an antibody? Best thanks in advance Alex K Alex Knisely, MD Consultant Histopathologist alex.knisely@kcl.ac.uk Institute of Liver Studies King's College Hospital Denmark Hill London SE5 9RS UK +44 (0)20 - 7346 - 3125 telefax +44 (0)20 - 7346 - 4627 office From ekaplan <@t> squ.edu.om Wed Jan 21 05:45:39 2004 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] invitation Message-ID: INVITATION (1st Announcement) College of Medicine & Health Sciences Pathology Department Electron Microscopy Unit In collaboration with Center for Community Service & Continuing Education Invites you to attend ?OMAN FIRST ELECTRON MICROSCOPY WORKSHOP? March 6 ? 9, 2004 Venue: Lecture Theater 1, Sultan Qaboos University Opening Ceremony: Saturday March 6 2004 at 9:00 a.m. Tentative Program Schedule: Day (1) Saturday, 6 March 2004 8:00-9:00 Registration, Coffee 9:00-9:45 Opening Ceremony 9:45-10:00 Break 10:00-11:00 Lecture 11:00-12:00 Lecture 12:00-12:30 Tour around EM Lab 12:30-14:00 Lunch 13:00-17:30 Sample preparation for TEM Day (2) Sunday, 7 March 2004 8:30-9:30 Lecture 9:30-10:30 Lecture 10:30-11:00 Break / Exhibition 11:00-12:00 Lecture 12:00-13:00 Lunch 13:00-17:00 Sample preparation for SEM 19:00- 21:00 Workshop Dinner Day (3) Monday, 8 March 2004 8:30-9:30 Lecture 9:30-10:30 Lecture 10:30-11:00 Break / Exhibition 11:00-12:00 Lecture 12:00-13:00 Lunch 13:00-17:00 Operation & Maintenance of Electron Microscopes Day (4) Tuesday, 9 March 2004 8:30-9:30 Lecture 9:30-10:30 Lecture 10:30-11:00 Break / Exhibition 11:00-14:00 Operation & Maintenance of Electron Microscopes 14:00-15:00 Lunch 17:00-21:00 Tour - Muscat area For registration and full details about the workshop please visit our web site address: http://www.squ.edu.om/med/NEWS/med/index.htm Please note: registration for SQU staff & students is free of charge. For further information please call workshop coordinator Mrs. Edna Ranada at 513333 Ext 3562/1737, or Fax 513419, or send an E-mail: ebranada@squ.edu.om ------------------------------------------------------------------------ With Compliments : Public Relations & Information Dep Contact : P.O. Box 50, P.C 123 Al-Khode. Tel : +968-515271, Fax : +968-513158 Email : squinfo@squ.edu.om From wayneholland1959 <@t> msn.com Wed Jan 21 05:55:23 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] HT Certified vs. HT non-certified Message-ID: 15 years experience >From: Histolady710@aol.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] HT Certified vs. HT non-certified >Date: Tue, 20 Jan 2004 19:13:26 EST > >This is for histology managers ! I'm curious as to which HT you would hire >- >a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years >experience. Thanks. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Find high-speed ‘net deals — comparison-shop your local providers here. https://broadband.msn.com From wayneholland1959 <@t> msn.com Wed Jan 21 06:01:59 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:27 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: I also am looking, however after an exhaustive search and comparisons. My choice is Ventana. >From: Mplhisto@aol.com >To: histonet@pathology.swmed.edu (histonet) >Subject: [Histonet] Automatic Immuno Stainers >Date: Tue, 20 Jan 2004 21:41:38 EST > > Our lab will be looking for an automatic Special/ Immuno stainer this >year. >I was curious to see which stainers you would recommend. We do a large >volume >of Immunos and specials and we are currently using the Cytologix. Any >feedback positive or negative would be greatly appreciated. > >Thanks, >Meredith Hale HT ( ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 From MAUGER <@t> email.chop.edu Wed Jan 21 07:12:39 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: No way to Ventana. They are not customer oriented at all. They do not provide good support, and keep you locked in to their reagent use. Dako is a very supportive company,and their autostainer is totally open,so you can use their reagents,or any other you may choose. They have a special stainer -Artisan- which is very easy to use with great results. I have been using Dako's autostainer for immuno for ten years,and have always been happy with results,service, and support.(They don"t pay me!!) Jo M. >>> "Wayne Holland" 01/21/04 07:01AM >>> I also am looking, however after an exhaustive search and comparisons. My choice is Ventana. >From: Mplhisto@aol.com >To: histonet@pathology.swmed.edu (histonet) >Subject: [Histonet] Automatic Immuno Stainers >Date: Tue, 20 Jan 2004 21:41:38 EST > > Our lab will be looking for an automatic Special/ Immuno stainer this >year. >I was curious to see which stainers you would recommend. We do a large >volume >of Immunos and specials and we are currently using the Cytologix. Any >feedback positive or negative would be greatly appreciated. > >Thanks, >Meredith Hale HT ( ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Wed Jan 21 07:42:01 2004 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: I'm sorry to hear that Joanne Mauger has had bad luck with Ventana. We've experienced just the opposite in terms of customer service; they have been very supportive of us and have worked with us in dealing with any staining issues that have come up. I don't have experience with their special stain module but the quality of the immunostains and in situ hybridization is superior and I highly recommend their products. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Joanne Mauger [mailto:MAUGER@email.chop.edu] Sent: Wednesday, January 21, 2004 7:13 AM To: Mplhisto@aol.com; wayneholland1959@msn.com; histonet@pathology.swmed.edu Subject: RE: [Histonet] Automatic Immuno Stainers No way to Ventana. They are not customer oriented at all. They do not provide good support, and keep you locked in to their reagent use. Dako is a very supportive company,and their autostainer is totally open,so you can use their reagents,or any other you may choose. They have a special stainer -Artisan- which is very easy to use with great results. I have been using Dako's autostainer for immuno for ten years,and have always been happy with results,service, and support.(They don"t pay me!!) Jo M. >>> "Wayne Holland" 01/21/04 07:01AM >>> I also am looking, however after an exhaustive search and comparisons. My choice is Ventana. >From: Mplhisto@aol.com >To: histonet@pathology.swmed.edu (histonet) >Subject: [Histonet] Automatic Immuno Stainers >Date: Tue, 20 Jan 2004 21:41:38 EST > > Our lab will be looking for an automatic Special/ Immuno stainer this >year. >I was curious to see which stainers you would recommend. We do a large >volume >of Immunos and specials and we are currently using the Cytologix. Any >feedback positive or negative would be greatly appreciated. > >Thanks, >Meredith Hale HT ( ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lynzjackson <@t> hotmail.com Wed Jan 21 08:09:13 2004 From: lynzjackson <@t> hotmail.com (lynsay jackson) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] necrosis IHC markers Message-ID: Hi, Does any body know any good immunohistochemistry markers for necrotic/dead cells? _________________________________________________________________ Tired of 56k? Get a FREE BT Broadband connection http://www.msn.co.uk/specials/btbroadband From katherine-walters <@t> uiowa.edu Wed Jan 21 08:27:22 2004 From: katherine-walters <@t> uiowa.edu (katherine-walters@uiowa.edu) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] peltier vs. refrigeration cold stage for sliding microtome Message-ID: <1074695242.400e8c4a844d8@webmail1.its.uiowa.edu> Dear Histologists, I am beginning the process of looking at cold stages for a sliding microtome. Does anyone have experience or opinions about the usage of peltier stages as opposed to refrigeration stages? I would also appreciate any information on which companies have these for sale. Thank you once again, Kathy Walters From DMBCMP <@t> aol.com Wed Jan 21 08:34:28 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Stanford Histo Supervisor Message-ID: <5b.45b21bda.2d3fe7f4@aol.com> Good Morning: I wonder if you would mind responding to my question about using melted paraffin...o not... in the embedding centre while embedding blocks? I worked at Stanford in 1998 while Pam was still supervisor, so I feel an attachment. Two of my pathologists trained there and we would like to know your present policy on this matter. If you have a minute, will you let me know? Thanks so much. If Marty is still there, will you say hello for me, as well as the other techs? Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Hospital From juan.gutierrez <@t> christushealth.org Wed Jan 21 08:38:31 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] HT Certified vs. HT non-certified Message-ID: You know you're openning a can of worms with this question. Why hasn't the OJT tech gotten registered yet? I would hire the certified tech and teach him/her how I want things done in my lab. My pre-emptive apologies to anyone this might offend. Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare San Antonio, TX -----Original Message----- From: Histolady710@aol.com [mailto:Histolady710@aol.com] Sent: Tue 1/20/2004 6:13 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] HT Certified vs. HT non-certified This is for histology managers ! I'm curious as to which HT you would hire - a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years experience. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wayneholland1959 <@t> msn.com Wed Jan 21 08:40:37 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: Sorry to hear of your problems with Ventana, I have had the opportunity in my 26 years to have a good relationship with both companies. However; a lot has to do with what suits your needs, along with your sales rep and their abilities. >From: "Joanne Mauger" >To: Mplhisto@aol.com,wayneholland1959@msn.com,histonet@pathology.swmed.edu >Subject: RE: [Histonet] Automatic Immuno Stainers >Date: Wed, 21 Jan 2004 08:12:39 -0500 > >No way to Ventana. They are not customer oriented at all. They do not >provide good support, and keep you locked in to their reagent use. >Dako is a very supportive company,and their autostainer is totally open,so >you can use their reagents,or any other you may choose. They have a special >stainer -Artisan- which is very easy to use with great results. I have been >using Dako's autostainer for immuno for ten years,and have always been >happy with results,service, and support.(They don"t pay me!!) >Jo M. > > >>> "Wayne Holland" 01/21/04 07:01AM >>> >I also am looking, however after an exhaustive search and comparisons. My >choice is Ventana. > > > >From: Mplhisto@aol.com > >To: histonet@pathology.swmed.edu (histonet) > >Subject: [Histonet] Automatic Immuno Stainers > >Date: Tue, 20 Jan 2004 21:41:38 EST > > > > Our lab will be looking for an automatic Special/ Immuno stainer this > >year. > >I was curious to see which stainers you would recommend. We do a large > >volume > >of Immunos and specials and we are currently using the Cytologix. Any > >feedback positive or negative would be greatly appreciated. > > > >Thanks, > >Meredith Hale HT ( ASCP) > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________________________________________ >Check out the coupons and bargains on MSN Offers! >http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _________________________________________________________________ High-speed users—be more efficient online with the new MSN Premium Internet Software. http://join.msn.com/?pgmarket=en-us&page=byoa/prem&ST=1 From juan.gutierrez <@t> christushealth.org Wed Jan 21 08:42:58 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: Ventana's Benchmark XT. It will do IHC, FISH and CISH in the same run. Juan -----Original Message----- From: Mplhisto@aol.com [mailto:Mplhisto@aol.com] Sent: Tue 1/20/2004 8:41 PM To: histonet Cc: Subject: [Histonet] Automatic Immuno Stainers Our lab will be looking for an automatic Special/ Immuno stainer this year. I was curious to see which stainers you would recommend. We do a large volume of Immunos and specials and we are currently using the Cytologix. Any feedback positive or negative would be greatly appreciated. Thanks, Meredith Hale HT ( ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Jan 21 08:43:27 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Stanford Histo Supervisor Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B4401979954@exch4.sjha.org> I have always embedded from hot melted paraffin, (and I am older than dirt). Would have no idea how it would work otherwise. If I cannot get to blocks in a reasonable amount of time, I set them out of the heat and remelt before embedding. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DMBCMP@aol.com Sent: Wednesday, January 21, 2004 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stanford Histo Supervisor Good Morning: I wonder if you would mind responding to my question about using melted paraffin...o not... in the embedding centre while embedding blocks? I worked at Stanford in 1998 while Pam was still supervisor, so I feel an attachment. Two of my pathologists trained there and we would like to know your present policy on this matter. If you have a minute, will you let me know? Thanks so much. If Marty is still there, will you say hello for me, as well as the other techs? Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From DonnaWillis <@t> texashealth.org Wed Jan 21 08:55:21 2004 From: DonnaWillis <@t> texashealth.org (Willis, Donna) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Remove from list server Message-ID: <5C6CBCCEB04B894C8BD15B312487F7B201A32EA5@ftwex01.txhealth.org> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From flemons <@t> bhset.org Wed Jan 21 08:54:17 2004 From: flemons <@t> bhset.org (Fran Lemons) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Cassette holders Message-ID: A while back I had asked if there was a company that made cassette racks for 12-20 cassettes like the kind Tissue tek used to make. I got a response and wrote down the name of the company and promptly lost the piece of paper. I think it was 'Golden" something. Any guesses? Thanks histo buddies- Fran From juan.gutierrez <@t> christushealth.org Wed Jan 21 09:01:05 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Cassette holders Message-ID: Golden State Histology Specialties, Inc. P.O. Box 279 Lexington, SC 29071 (803)996-9075 gldnsthisto@aol.com -----Original Message----- From: Fran Lemons [mailto:flemons@bhset.org] Sent: Wed 1/21/2004 8:54 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Cassette holders A while back I had asked if there was a company that made cassette racks for 12-20 cassettes like the kind Tissue tek used to make. I got a response and wrote down the name of the company and promptly lost the piece of paper. I think it was 'Golden" something. Any guesses? Thanks histo buddies- Fran _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Wed Jan 21 09:04:58 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin Message-ID: Dannie, I put my blocks in a warm reservoir with no melted paraffin. I process animal tissue mostly rodent and this to me is gentler on the brain tissue and I have found no difference in the cutting or staining but have not done a blinded controlled study. I work on neonatal mouse eyes at this time and wrap them in lens paper or but in histo gel to facilitate the orientation and ensure the specimen does not get lost! My opinion only! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: DMBCMP@aol.com [mailto:DMBCMP@aol.com] Sent: Tuesday, January 20, 2004 8:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding W/WO Melted Paraffin Hi, everyone: In order to settle a difference of opinion between generations of trained Histotechs in my lab, may I have feedback from anyone interested in responding? Do you cover the blocks in melted paraffin in the embedding centre reservior and hold them that way while embedding? or.... Do you dump them in the warm reservior "dry" (not covered in melted paraffin) and embed them that way? Do you consider this "dry" method as bad technique since a tiny biopsy specimen MAY not be noticed as the paraffin quickly solidifies? We have a dispute. I have researched every book I can find and there is no reference to it anywhere. A newly trained tech that came to work for us said no mention was made during her training period. Some techs did...and some didn't. As a tech of nearly 27 years, I find this practice to be just asking for trouble. I was trained to keep everything melted. There seems to be some argument against keeping the cassettes in the melted paraffin, claiming it "cooks" the biopsies. I don't buy it, but what are the opinions of others? It seems so basic to me. I hope this does not come across as frivilous. Thanks for your input. Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Medical Centre Fresno, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Wed Jan 21 09:12:42 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin Message-ID: At my former hospital and here the blocks are embedded "dry". Biopsies are found just fine. On the hot plate any paraffin is melted so confusing paraffin for a biopsy doesn't happen. The exception is maybe using paper instead of mesh cassettes, and then you just scrape everything on the paper into the cassette with a scalpel. I can't tell you how many time techs have scraped the paper and didn't see anything, but embedded the block anyway and there is something (and the correct something) on the cut slide. I think either way is fine. It is a matter of what folks are used to. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DMBCMP@aol.com Sent: Tuesday, January 20, 2004 9:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding W/WO Melted Paraffin Hi, everyone: In order to settle a difference of opinion between generations of trained Histotechs in my lab, may I have feedback from anyone interested in responding? Do you cover the blocks in melted paraffin in the embedding centre reservior and hold them that way while embedding? or.... Do you dump them in the warm reservior "dry" (not covered in melted paraffin) and embed them that way? Do you consider this "dry" method as bad technique since a tiny biopsy specimen MAY not be noticed as the paraffin quickly solidifies? We have a dispute. I have researched every book I can find and there is no reference to it anywhere. A newly trained tech that came to work for us said no mention was made during her training period. Some techs did...and some didn't. As a tech of nearly 27 years, I find this practice to be just asking for trouble. I was trained to keep everything melted. There seems to be some argument against keeping the cassettes in the melted paraffin, claiming it "cooks" the biopsies. I don't buy it, but what are the opinions of others? It seems so basic to me. I hope this does not come across as frivilous. Thanks for your input. Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Medical Centre Fresno, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jan 21 09:12:56 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Stanford Histo Supervisor Message-ID: I, too, put molten paraffin in the transfer tray with the cassettes as they come off the processor. However, we just got a brand new embedding center and I was told by the rep. that I am in the minority. Most people it would seem just put them in the heated tray with no paraffin. The temperature keeps them warm enough to embed, or so I am told. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 21, 2004 9:43 AM To: 'DMBCMP@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stanford Histo Supervisor I have always embedded from hot melted paraffin, (and I am older than dirt). Would have no idea how it would work otherwise. If I cannot get to blocks in a reasonable amount of time, I set them out of the heat and remelt before embedding. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DMBCMP@aol.com Sent: Wednesday, January 21, 2004 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stanford Histo Supervisor Good Morning: I wonder if you would mind responding to my question about using melted paraffin...o not... in the embedding centre while embedding blocks? I worked at Stanford in 1998 while Pam was still supervisor, so I feel an attachment. Two of my pathologists trained there and we would like to know your present policy on this matter. If you have a minute, will you let me know? Thanks so much. If Marty is still there, will you say hello for me, as well as the other techs? Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From cfavara <@t> niaid.nih.gov Wed Jan 21 09:10:14 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: I use Ventana do only research. I find them very responsive to anything I want to do, customer support is the best I have ever experienced. I am out in the boonies so that Is a very important feature for me! Know reagents are pricey but I find it worth while! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Mplhisto@aol.com [mailto:Mplhisto@aol.com] Sent: Tuesday, January 20, 2004 7:42 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Automatic Immuno Stainers Our lab will be looking for an automatic Special/ Immuno stainer this year. I was curious to see which stainers you would recommend. We do a large volume of Immunos and specials and we are currently using the Cytologix. Any feedback positive or negative would be greatly appreciated. Thanks, Meredith Hale HT ( ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Jan 21 08:56:40 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: Interesting! We have quite the opposite situation here in Texas. I have yet to meet my DAKO rep, even though I have one immunostainer and continue to use some of their primaries. My Ventana rep is always in contact with me and comes by at least once a month to check up on us. We are also able to run any reagent we want on the Benchmarks, so I don't know where you get the idea about being locked up into their reagents. Open your mind and explore the possibilities. I did and I think I'm better off for it. Like you I used to put Ventana down and praise DAKO, not anymore. DAKO fell asleep a couple of years back and the guys from Tucson knew what to do and how to do it well. (They don't pay me either). Good luck, Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Joanne Mauger [mailto:MAUGER@email.chop.edu] Sent: Wed 1/21/2004 7:12 AM To: Mplhisto@aol.com; wayneholland1959@msn.com; histonet@pathology.swmed.edu Cc: Subject: RE: [Histonet] Automatic Immuno Stainers No way to Ventana. They are not customer oriented at all. They do not provide good support, and keep you locked in to their reagent use. Dako is a very supportive company,and their autostainer is totally open,so you can use their reagents,or any other you may choose. They have a special stainer -Artisan- which is very easy to use with great results. I have been using Dako's autostainer for immuno for ten years,and have always been happy with results,service, and support.(They don"t pay me!!) Jo M. >>> "Wayne Holland" 01/21/04 07:01AM >>> I also am looking, however after an exhaustive search and comparisons. My choice is Ventana. >From: Mplhisto@aol.com >To: histonet@pathology.swmed.edu (histonet) >Subject: [Histonet] Automatic Immuno Stainers >Date: Tue, 20 Jan 2004 21:41:38 EST > > Our lab will be looking for an automatic Special/ Immuno stainer this >year. >I was curious to see which stainers you would recommend. We do a large >volume >of Immunos and specials and we are currently using the Cytologix. Any >feedback positive or negative would be greatly appreciated. > >Thanks, >Meredith Hale HT ( ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Wed Jan 21 09:28:53 2004 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Older than dirt comment Message-ID: Morning all, I had to laugh at Joyce's "older than dirt comment". At Christmas, my youngest grandson asked me if I was alive when the dinosaurs were still around. It's nice to know that there are some of us of advanced age who are still working in our chosen field. Jacquie Poteete MT(ASCP) QIHC, Lead Technologist IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From JWEEMS <@t> sjha.org Wed Jan 21 09:43:20 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Off topic - Older than dirt comment Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B440197995B@exch4.sjha.org> There's an email test making the rounds that certified me as older than dirt. :>) Isn't is wonderful when our grandchildren ask US about the good old days? But then, shows you what I know with the most recent posts about putting blocks in dry heat before embedding. Learn something new everyday! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Poteete, Jacquie A. Sent: Wednesday, January 21, 2004 10:29 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Older than dirt comment Morning all, I had to laugh at Joyce's "older than dirt comment". At Christmas, my youngest grandson asked me if I was alive when the dinosaurs were still around. It's nice to know that there are some of us of advanced age who are still working in our chosen field. Jacquie Poteete MT(ASCP) QIHC, Lead Technologist IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From RossS <@t> BaylorHealth.edu Wed Jan 21 09:44:38 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Automatic Immuno Stainers Message-ID: Customer service really depends on your local Rep. My Rep in DC for DAKO was great. My DAKO Rep here leaves a bit to be desired lets say. As for which machine is best, it depends on your needs and what antibodies you are running. It also depends on how knowledgable your immuno techs are. On average I would say for a lab with less experienced immuno techs where tech time is the biggest problem and most of the immuno's that are run are pretty standard then the Ventana Benchmark XT is best. If you do more obscure immuno stains and have experienced techs who have time to do the pretreatment themselves then on average Dako is better. That is not to say that obscure antibodies can't be done on the Ventana, they just often take more patience and persistance than with the Dako stainer in my experience. If you have the time to work with a brand new instrument then I would look at the Bond from Vision Biosystems. The Bond seems to take the best of Dako and the best of Ventana and put it together in one instrument. I just wish they had the Insitu and de-paraffinization available for me to evaluate now. The basic version of the machine seems to work great so far. We currently use the Ventana ES, but we are evaluating all three stainers right now. I wish we could get more than one because they all have their strengths and weaknesses. It is just a matter of deciding which strengths are most important and which weaknesses you can live with. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GUTIERREZ, JUAN Sent: Wednesday, January 21, 2004 8:57 AM To: Joanne Mauger; Mplhisto@aol.com; wayneholland1959@msn.com; histonet@pathology.swmed.edu Subject: RE: [Histonet] Automatic Immuno Stainers Interesting! We have quite the opposite situation here in Texas. I have yet to meet my DAKO rep, even though I have one immunostainer and continue to use some of their primaries. My Ventana rep is always in contact with me and comes by at least once a month to check up on us. We are also able to run any reagent we want on the Benchmarks, so I don't know where you get the idea about being locked up into their reagents. Open your mind and explore the possibilities. I did and I think I'm better off for it. Like you I used to put Ventana down and praise DAKO, not anymore. DAKO fell asleep a couple of years back and the guys from Tucson knew what to do and how to do it well. (They don't pay me either). Good luck, Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Joanne Mauger [mailto:MAUGER@email.chop.edu] Sent: Wed 1/21/2004 7:12 AM To: Mplhisto@aol.com; wayneholland1959@msn.com; histonet@pathology.swmed.edu Cc: Subject: RE: [Histonet] Automatic Immuno Stainers No way to Ventana. They are not customer oriented at all. They do not provide good support, and keep you locked in to their reagent use. Dako is a very supportive company,and their autostainer is totally open,so you can use their reagents,or any other you may choose. They have a special stainer -Artisan- which is very easy to use with great results. I have been using Dako's autostainer for immuno for ten years,and have always been happy with results,service, and support.(They don"t pay me!!) Jo M. >>> "Wayne Holland" 01/21/04 07:01AM >>> I also am looking, however after an exhaustive search and comparisons. My choice is Ventana. >From: Mplhisto@aol.com >To: histonet@pathology.swmed.edu (histonet) >Subject: [Histonet] Automatic Immuno Stainers >Date: Tue, 20 Jan 2004 21:41:38 EST > > Our lab will be looking for an automatic Special/ Immuno stainer this >year. >I was curious to see which stainers you would recommend. We do a large >volume >of Immunos and specials and we are currently using the Cytologix. Any >feedback positive or negative would be greatly appreciated. > >Thanks, >Meredith Hale HT ( ASCP) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susan.wells <@t> bms.com Wed Jan 21 10:17:43 2004 From: susan.wells <@t> bms.com (Susan Q Wells) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] apotosis marker in mice Message-ID: <400EA627.3CDD1DCC@bms.com> Hello fellow histonetters - Does anyone have a straightforward protocol for an apotosis marker to use on frozen or paraffin mice sections.I used the TUNEL stain a number of years ago but I'm thinking there may be an antibody out there that works just as well? Thanks in advance for your time, Sue Wells HT(ASCP),QIHC From laurie.colbert <@t> huntingtonhospital.com Wed Jan 21 10:35:09 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Leica Cassette Printer Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C91@EXCHANGE1.huntingtonhospital.com> Does anyone out there have or tried out the Leica IP C Cassetter Printer? If so, can you provide any feedback? I am still trying to find a cassette labeler that will work for us. We have tried the Shandon, the TBS, and the new Sakura, but I wasn't totally happy with any of them. Does anyone have info on the annual CSH meeting this year? Laurie Colbert Huntington Hospital Pasadena, CA From JWEEMS <@t> sjha.org Wed Jan 21 10:37:06 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Leica Cassette Printer Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B4401979963@exch4.sjha.org> Just fyi - Leica is the same intrument as the Sakura. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, January 21, 2004 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Cassette Printer Does anyone out there have or tried out the Leica IP C Cassetter Printer? If so, can you provide any feedback? I am still trying to find a cassette labeler that will work for us. We have tried the Shandon, the TBS, and the new Sakura, but I wasn't totally happy with any of them. Does anyone have info on the annual CSH meeting this year? Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From MAUGER <@t> email.chop.edu Wed Jan 21 10:36:49 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Stanford Histo Supervisor Message-ID: I have found that either way works fine, as long as your forceps are hot. We put tint biopsies on sponges, and they heat up on the embedding center so tint tissues is pretty easy to see! >>> "Bartlett, Jeanine" 01/21/04 10:12AM >>> I, too, put molten paraffin in the transfer tray with the cassettes as they come off the processor. However, we just got a brand new embedding center and I was told by the rep. that I am in the minority. Most people it would seem just put them in the heated tray with no paraffin. The temperature keeps them warm enough to embed, or so I am told. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 21, 2004 9:43 AM To: 'DMBCMP@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stanford Histo Supervisor I have always embedded from hot melted paraffin, (and I am older than dirt). Would have no idea how it would work otherwise. If I cannot get to blocks in a reasonable amount of time, I set them out of the heat and remelt before embedding. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DMBCMP@aol.com Sent: Wednesday, January 21, 2004 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stanford Histo Supervisor Good Morning: I wonder if you would mind responding to my question about using melted paraffin...o not... in the embedding centre while embedding blocks? I worked at Stanford in 1998 while Pam was still supervisor, so I feel an attachment. Two of my pathologists trained there and we would like to know your present policy on this matter. If you have a minute, will you let me know? Thanks so much. If Marty is still there, will you say hello for me, as well as the other techs? Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Jan 21 10:43:03 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BD7F@EXCHANGE1.huntingtonhospital.com> We put our cassettes in the reservoir "dry." As long as the reservoir is warm enough and you keep the lid closed, the cassettes will stay warm and melted. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: DMBCMP@aol.com [mailto:DMBCMP@aol.com] Sent: Tuesday, January 20, 2004 7:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding W/WO Melted Paraffin Hi, everyone: In order to settle a difference of opinion between generations of trained Histotechs in my lab, may I have feedback from anyone interested in responding? Do you cover the blocks in melted paraffin in the embedding centre reservior and hold them that way while embedding? or.... Do you dump them in the warm reservior "dry" (not covered in melted paraffin) and embed them that way? Do you consider this "dry" method as bad technique since a tiny biopsy specimen MAY not be noticed as the paraffin quickly solidifies? We have a dispute. I have researched every book I can find and there is no reference to it anywhere. A newly trained tech that came to work for us said no mention was made during her training period. Some techs did...and some didn't. As a tech of nearly 27 years, I find this practice to be just asking for trouble. I was trained to keep everything melted. There seems to be some argument against keeping the cassettes in the melted paraffin, claiming it "cooks" the biopsies. I don't buy it, but what are the opinions of others? It seems so basic to me. I hope this does not come across as frivilous. Thanks for your input. Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Medical Centre Fresno, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Wed Jan 21 10:45:54 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] apotosis marker in mice Message-ID: There are a number of the caspaces that will work for you. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Susan Q Wells [mailto:susan.wells@bms.com] Sent: Wednesday, January 21, 2004 9:18 AM To: histonet@pathology.swmed.edu Subject: [Histonet] apotosis marker in mice Hello fellow histonetters - Does anyone have a straightforward protocol for an apotosis marker to use on frozen or paraffin mice sections.I used the TUNEL stain a number of years ago but I'm thinking there may be an antibody out there that works just as well? Thanks in advance for your time, Sue Wells HT(ASCP),QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TIGERGIL <@t> aol.com Wed Jan 21 10:57:57 2004 From: TIGERGIL <@t> aol.com (TIGERGIL@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] forensic section membership moacad.org Message-ID: <1ed.17c98023.2d400995@aol.com> Missourians and neighbors working in the forensic sciences - and i include forensic histology- are welcome to join our new re-organized forensic section of the Missouri Academy of Science...it is a place to present your research and new findings and techniques to a formal audience once a year...and a place for your students to present their first paper...so go to www.moacad.org and sign up and don't forget to join the forensic section...ps there are other sections open to you as well...this is a great way to maintain your skills and interest in science...by giving a paper every year..tigergil@aol.com gil corrigan 11801 hidden lake st louis mo 63138 From dbpiontek <@t> hsc.wvu.edu Wed Jan 21 10:58:51 2004 From: dbpiontek <@t> hsc.wvu.edu (Denise Bland-Piontek) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Block Pulling Fees Message-ID: Just wondering how many clinical labs bill for pulling blocks? Our clinical lab just began charging $2.00 per block. Even our pathologists must pay when having blocks pulled for research or education. Just wondering if this is standard? Thanks, Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator WVU From kappeler <@t> patho.unibe.ch Wed Jan 21 11:09:29 2004 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] C4d antibody References: Message-ID: <013501c3e041$54ac7430$27955c82@patho.unibe.ch> Hi Annette rb-a-C4d, Biomedica (http://www.biomedica.co.at/; in the US try http://www.bmgrp.com/) Cat.No. BI-RC4D. Working dilution around 1:25-1:50. Requires HIER, pressure cooker with citrate buffer, pH 6.0, may be okay, better results with microwave and 100 mM Tris - 5% urea, pH 9.5 (strange stuff, but it works). Make sure to use a biotin-independent visualization system or use an avidin-biotin block. Good luck! Regards, Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Featherstone, Annette" To: "'Instrumedics'" ; "HistoNet Server" Sent: Tuesday, January 20, 2004 7:41 PM Subject: [Histonet] C4d antibody > Hi > > Anyone out there using C4d antibody? Have to work it up and want to know if > there are any tips to make this work as they haven't been able to get it to > work right. Manufacturer recommends Alk Phos, but I was hoping to get it to > work with DAB. Any suggestions? Thanks > Annette Featherstone HT/MLT From gcallis <@t> montana.edu Wed Jan 21 11:13:56 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin Message-ID: <3.0.6.32.20040121101356.00bd6418@gemini.msu.montana.edu> >Not frivilous at all. > >I would never put cassettes with tissues IN my embedding center reservoir, that is asking for the proverbial carryovers! Pieces of tiny tissues can come out of those cassettes and cross contaminate a new block when paraffin is dispensed into another samples embedding mold! > >There is no law to say you can't put melted paraffin in the cassette holding reservoir. In fact, when doing FDA/GLP, this is exactly what we did to put a thermometer in that melted paraffin to know reservoir temperature. Readout temperatures were not accurate and we were required to use a certified calibrated thermometer. > >We place cassettes in the dry reservoir all the time (there is always some melted paraffin in there!) without problems. But that reservoir is set at 60C, the melting point of the paraffin, so if you are experiencing solid paraffin coming from dry reservoir, readjust the temperature so you samples stay melted. > >If you use colored cassettes for biopsies, and worried about cooking them, the color will alert you to embed these biopsies first. Cooking is the result of excessive temperature, so make sure the temp is adjusted on all surfaces of your embedding center, also the paraffin dispenser, and dry reservoir. > > > >At 10:37 PM 1/20/2004 EST, you wrote: >>Hi, everyone: >>In order to settle a difference of opinion between generations of trained >>Histotechs in my lab, may I have feedback from anyone interested in responding? >>Do you cover the blocks in melted paraffin in the embedding centre reservior >>and hold them that way while embedding? or.... >>Do you dump them in the warm reservior "dry" (not covered in melted paraffin) >>and embed them that way? >>Do you consider this "dry" method as bad technique since a tiny biopsy >>specimen MAY not be noticed as the paraffin quickly solidifies? >>We have a dispute. I have researched every book I can find and there is no >>reference to it anywhere. A newly trained tech that came to work for us said >>no mention was made during her training period. Some techs did...and some >>didn't. As a tech of nearly 27 years, I find this practice to be just asking for >>trouble. I was trained to keep everything melted. There seems to be some >>argument against keeping the cassettes in the melted paraffin, claiming it "cooks" >>the biopsies. I don't buy it, but what are the opinions of others? >>It seems so basic to me. I hope this does not come across as frivilous. >>Thanks for your input. >> >>Dannie Blake HT(ASCP) Histology Lead Tech >>Fresno Community Medical Centre >>Fresno, California >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From mrsgbd2001 <@t> yahoo.com Wed Jan 21 11:14:24 2004 From: mrsgbd2001 <@t> yahoo.com (Gareth Davis) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] peltier vs. refrigeration cold stage for sliding microtome In-Reply-To: <1074695242.400e8c4a844d8@webmail1.its.uiowa.edu> Message-ID: <20040121171424.85171.qmail@web13302.mail.yahoo.com> About a year ago, the company I worked for bought a sliding microtome and freezing stage from Richard-Allen Scientific. I was very satisfied with the both the Micon microtome and the freezing stage. I do remember that Richard-Allen had to had to do a special order on the freezing stage, becuase they didn't keep it in stock, but they were more than helpful with finding it. I was the stage that had the "cups" on either end to hold the dry ice chips. Hope this helps. Gareth Blaeuer Davis Histotechinican PathGroup Nashville, Tennessee katherine-walters@uiowa.edu wrote: Dear Histologists, I am beginning the process of looking at cold stages for a sliding microtome. Does anyone have experience or opinions about the usage of peltier stages as opposed to refrigeration stages? I would also appreciate any information on which companies have these for sale. Thank you once again, Kathy Walters _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From MinHan.Tan <@t> vai.org Wed Jan 21 11:20:46 2004 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Kappa and interobserver variation Message-ID: <74D0F0AB07F2E647A02D839ED79520F9B482B9@VAIEXCH02.vai.org> Hi, I'd like to ask a mildly peripheral question on histopathology. ;) Does anyone know of a friendly program for deriving the weighted kappa statistic (for the measurement of inter-pathologist variation)? SPSS 11.5 doesn't seem to have weighted kappa (only unweighted), and I don't have SAS. Thanks! Min-Han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. From JWEEMS <@t> sjha.org Wed Jan 21 11:10:07 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Block Pulling Fees Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B4401979969@exch4.sjha.org> We only pay for STAT requests. That is part of the service of our off-site storage company. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Denise Bland-Piontek Sent: Wednesday, January 21, 2004 11:59 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Block Pulling Fees Just wondering how many clinical labs bill for pulling blocks? Our clinical lab just began charging $2.00 per block. Even our pathologists must pay when having blocks pulled for research or education. Just wondering if this is standard? Thanks, Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator WVU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From Jackie.O'Connor <@t> abbott.com Wed Jan 21 11:25:43 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] apotosis marker in mice Message-ID: I use Caspase-3 routinely for apoptosis. I use an antibody on zinc fixed paraffin embedded tissues, but there are some out there for FFPE. Much easier than TUNEL, and more specific. Susan Q Wells Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2004 10:17 AM To: histonet@pathology.swmed.edu cc: Subject: [Histonet] apotosis marker in mice Hello fellow histonetters - Does anyone have a straightforward protocol for an apotosis marker to use on frozen or paraffin mice sections.I used the TUNEL stain a number of years ago but I'm thinking there may be an antibody out there that works just as well? Thanks in advance for your time, Sue Wells HT(ASCP),QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrsgbd2001 <@t> yahoo.com Wed Jan 21 11:28:28 2004 From: mrsgbd2001 <@t> yahoo.com (Gareth Davis) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Working Saturdays In-Reply-To: <9E75DAB5F369D84ABF84FAB7A0243B4401979923@exch4.sjha.org> Message-ID: <20040121172828.37965.qmail@web13306.mail.yahoo.com> In our lab at PathGroup, the day shift takes turns to do Saturdays. In our case we only need one person, because don't do any special stains or IHC. We basically just cut and get slides ready to be done early Monday moring. But, the person who is scheduled to work on a Saturday gets the previous Monday (so that the time works). Good luck. Gareth Blaeuer Davis Histotechinican PathGroup Nashville, Tennessee "Weems, Joyce" wrote: An unofficial survey: How many hospitals are working Saturdays? And what is the case load of those that do? Thanks for your help! Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph’s Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes From Luis.Chiriboga <@t> med.nyu.edu Wed Jan 21 11:34:12 2004 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Hep C Message-ID: Hi All is anyone using/trying/ have an antibody for Hep C Thanks Luis From tpmorken <@t> labvision.com Wed Jan 21 11:34:45 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] HT Certified vs. HT non-certified Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2035762@usca0082k08.labvision.apogent.com> Yes, the age old question. As usual, it depends...... I worked with an uncertified histotech of over 15 years experience who could do the usual special stains well, and was an excellet cutter, but who could not seem to develop methods on his own, write any meaningful procedures or even read very well. Basically he was one of those with one year of experience repeated 15 times. Although he was not certified it was not for lack of trying - he tried five times and couldn't pass the written test (which goes along with the inability to develop procedures, I think, and he only attempted certification when others in the lab started a study group). But he had some skill so he was an asset to the lab as a general bench worker. I have also worked with formally-trained (ie, completed a course), certified, very interested, but who were simply mediocre and never advanced. Then there are those OJT trained techs who got certified by their own hard work and were absolutely excellent and advanced almost at will (Indeed, I recently interviewed a non-certified histotech of only two years of experience (and no formal histotech courses) who had accomplished more in that two years than most techs do in their entire careers!). So, it depends on the person. I tend to be concerned about a person who has worked in the field for 15 years and is not certified. That is due either to lack of interest, or lack of ability in some way. A person who has received the certificaiton has proven their basic skill AND at least some potential for personal advancement. After all, the test is a personal endeavor; the person has to do the lab work, the reading, and the test. You don't get certified just because you occupied a space in the lab for 15 years. And if the issue is trainability, probably the certified person is going to be more trainiable than someone who can't, or won't attempt to, get certified So I guess the short answer is to look into their past very carefully and see what they have been doing. Never base it simply on years (long or short) or certification alone. Tim Morken -----Original Message----- From: Histolady710@aol.com [mailto:Histolady710@aol.com] Sent: Tuesday, January 20, 2004 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Certified vs. HT non-certified This is for histology managers ! I'm curious as to which HT you would hire - a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years experience. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barbara.Harris <@t> UTSouthwestern.edu Wed Jan 21 11:39:48 2004 From: Barbara.Harris <@t> UTSouthwestern.edu (Barbara Harris) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Recutting Old GMA tissue blocks Message-ID: Need helpful tips,protocols,and experienced advise, on how to resoften or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will be sectioning thin section for teaching purposes.Thanks in advance for any and all responses. From naje1972 <@t> yahoo.com Wed Jan 21 12:09:02 2004 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Part time jobs in chicago Message-ID: <20040121180902.58366.qmail@web41708.mail.yahoo.com> I have a question, are there any part time Histology in the chicago land area. If so please e-mail at cynthiahaynes@hotmail.com or call me at 1-773-342-1559. Thanks in advance Cynthia Haynes H.T. __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus From MAUGER <@t> email.chop.edu Wed Jan 21 11:44:43 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Hep C Message-ID: Luis, Novocastra(Vector) has a good monoclonal Hep C antibody. Jo >>> "Luis Chiriboga" 01/21/04 12:34PM >>> Hi All is anyone using/trying/ have an antibody for Hep C Thanks Luis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Jan 21 11:32:04 2004 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Block Pulling Fees References: Message-ID: <400EB794.6040205@pathology.washington.edu> We also charge for pulling slides and blocks, but not for our pathologists. I don't recall the $ amounts. Denise Bland-Piontek wrote: >Just wondering how many clinical labs bill for pulling blocks? Our >clinical lab just began charging $2.00 per block. Even our pathologists >must pay when having blocks pulled for research or education. Just >wondering if this is standard? Thanks, >Denise Bland-Piontek, HTL(ASCP) >Tissue Bank & Research Administrator >WVU > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center 206-543-4823 From scoop <@t> mail.nih.gov Wed Jan 21 12:38:15 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] embarrassing question Message-ID: We recently had someone perfuse mice with 0.2M Sorensen's PBS instead of 0.1M (with 4% formalin). I would expect the cells to shrivel, but when I looked at H&E's of the sections they didn't look any worse than some of our other blocks which were done correctly. Does anyone know what happens when you perfuse tissues with 2x Sorensen's PBS or have experience with this problem to tell me what problems I should look out for - eg. damage to the tissues that might not be obvious on a quick scan of an H&E? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From SCheasty <@t> ahs.llumc.edu Wed Jan 21 12:42:07 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Question re: High School and 2 years experience not accepted as HT criteria starting 2005 Message-ID: <2E50F33F91EEDA46A77BC3B2575BB09105873F@mars.llumc.edu> RE: the accreditation route of "High school graduation (or equivalent) plus two years full time acceptable experience in histopathology" that is discontinued in 2005... Does that mean that you have to have your 2 years experience completed by 2005, or you have to have started your 2 years experience by 2005? Or maybe you have to apply by 2005? Thanks, Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From acjanes <@t> bu.edu Wed Jan 21 13:11:17 2004 From: acjanes <@t> bu.edu (Amy Janes) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] How to eliminate holes in mouse brain tissue? Message-ID: Hi, I am writing to see if I can get some advice on how to eliminate holes I am seeing in my tissue. I am using tissue from the mouse olfactory bulb and brain for fos ICC. Unfortunately I have been unable to see any fos because my tissue is full of little holes and therefore I have no actual cells. I have determined that it is not the ICC that is causing the holes because they are present before staining. So I am assuming I am doing something wrong during fixation and cryoprotection. I perfuse the animal with 4% para at a rate of 3.5ml/min and I give the animal around 50mls. I then post fix with 4% para overnight and then cryoprotect with 30% sucrose until the brain drops (usually overnight). The brain is then imbedded in OCT and put in the cryostat at -20C to freeze and cut at 20microns. I typically do not perfuse with PBS before para but others in my lab have tried perfusing with and without PBS and have found no difference in tissue quality. Any suggestions as to how I can fix this? Thanks, Amy From jqb7 <@t> cdc.gov Wed Jan 21 13:19:16 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] looking for antibody Message-ID: Does anyone know where I can find a commercial source for streptobacillus? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 From CTague <@t> ahs.llumc.edu Wed Jan 21 13:24:51 2004 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Histology Part-Timers? Message-ID: how do i apply? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cheasty, Sandra Sent: Tuesday, January 20, 2004 15:47 To: HistoNet (E-mail) Subject: [Histonet] Histology Part-Timers? There is an opening for a part-time histologist in the San Bernardino area of southern California. * 6:30-10:30 M-F * Plenty of time left in the day to get to the beach, spot celebrities, etc. * Well established lab, routine histology * Numerous pot lucks * Friendly co-workers Please e-mail me if you are interested.... Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From mcauliff <@t> umdnj.edu Wed Jan 21 13:31:29 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] How to eliminate holes in mouse brain tissue? In-Reply-To: References: Message-ID: <400ED391.1010208@umdnj.edu> Hi Amy: Your problem is relatively common, you are freezing the tissue too slowly. Ice crystals form and when the ice melts, it leaves holes behind. You will never get good results by putting the tissue in a cryostat at -20C or even -50C , air does not conduct heat fast enough. Freeze the tissue on a rapid freezing stage and/or surround it with dry ice 'snow' or partially submerge a metal chuck (with the tissue on it) in 2-methylbutane previously cooled with liquid nitrogen or dry ice. Then put the tissue in the cryostat and have a cup of coffee while it equilibrates to cryostat temp. Geoff Amy Janes wrote: >Hi, >I am writing to see if I can get some advice on how to eliminate holes I >am seeing in my tissue. >I am using tissue from the mouse olfactory bulb and brain for fos ICC. >Unfortunately I have been unable to see any fos because my tissue is full >of little holes and therefore I have no actual cells. I have determined >that it is not the ICC that is causing the holes because they are present >before staining. So I am assuming I am doing something wrong during >fixation and cryoprotection. I perfuse the animal with 4% para at a rate >of 3.5ml/min and I give the animal around 50mls. I then post fix with 4% >para overnight and then cryoprotect with 30% sucrose until the brain drops >(usually overnight). The brain is then imbedded in OCT and put in the >cryostat at -20C to freeze and cut at 20microns. I typically do not >perfuse with PBS before para but others in my lab have tried perfusing >with and without PBS and have found no difference in tissue quality. Any >suggestions as to how I can fix this? > >Thanks, > Amy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From nick.kirk3 <@t> btopenworld.com Wed Jan 21 13:36:18 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin In-Reply-To: <002401c3e03a$caf3b4c0$054dbad0@hppav> Message-ID: George We send all of ours away now as we do so few it's difficult to retain the level of expertise required. thanks for the offer though. As for the Robin Hood boyhood dreams, well sorry to burst your bubble but the Earl of Huntingdon was a Victorian invention that got into the legend and was then firmly concreted into the myth by the likes of Errol Flynn (who incidentally was a jobbing actor at the Northampton Repertory Theatre just a few miles down the road, before he made it big in Hollywood). The real Earl of Huntingdon who lived at the time of the Magna Carta when "The Hooded Man" was supposed to have lived was David de HUNTINGDON Earl of Huntingdon (1144-1219) and not old "Bob Hood" The Earl was the son of the King of Scotland. There was a castle at Huntingdon, but it was destroyed by King Henry II (Richard I and John's father) when it transpired that the Earl's family plotted to overthrow him. That may have something to do with how he got mixed up in the legend. All that remains of it now is a grassy hump in a park in the town. Incidentally the Earls descendents were on the side of the Royalists during the English Civil War and another son of Huntingdon, one Oliver Cromwell, laid siege to their nice new castle at Ashby-de-la-Zouch in Leicestershire and destroyed it. So the Earls of Huntingdon never had much luck with castles. Sherwood Forest is also about 70 miles away. Not far these days, but in 1205, a very long way away. Here endeth today's History lecture. Tomorrow, another son of Huntingdon, the diarist Samuel Pepys. Nicke Kirke Ye Head Purveyor of leeches and strange pungent unctures Histopathology Hinchingbrooke Hospital for ye sicke and Infirmed Huntingdon Merry Olde England -----Original Message----- From: George Cole [mailto:georgecole@ev1.net] Sent: 21 January 2004 16:23 To: 'Nick Kirk' Subject: RE: [Histonet] Embedding W/WO Melted Paraffin Nick I've been sending out packets of new more effective muscle and nerve biopsy methods to histotechs since last July. I have your name on my list from a few months back, but it doesn't show that I sent you a packet. If muscle biopsies and/or nerve biopsies are done there in your histopathology lab at Hunchingbrooke Hospital, I would be happy to send you one of these packets. THERE IS NO CHARGE FOR IT. You would be the 100th histotech to get one. I need the complete address of your institution---the post office is strict in that. I only wish I could bring it myself. As a child, I read every Robin Hood story ever written. I would go deep into Sherwood Forest, cut myself a good stout cudgel (actually, buy one from a lumber mill so as not to bother the woods)take up a truncheon of good stout brown pepsi cola and toast the discomfort of the sheriff of Nottingham under a tree close to the stream where Big John and Robin met with quarter staves a-knocking. georgecole@ev1.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nick Kirk Sent: Tuesday, January 20, 24 10:20 PM To: Histonet Subject: RE: [Histonet] Embedding W/WO Melted Paraffin Dannie I agree with you entirely. The argument that the biopsies will "cook" is totally spurious, after all, they've been sitting in molten paraffin wax on the processing machine for several hours before reaching the embedding stage so a few more minutes will have no effect. Also speaking from personal experience, embedding a "wet" piece of tissue is much easier than embedding a "warm but dry" piece of tissue. This is especially useful when you have a small piece of tissue requiring a particular orientation. The point you make about small biopsies is also a good one. You might be able to get away with using the "warm but dry" technique if you only had a few blocks to embed, but any lab with a sizeable through put should avoid that method of embedding in my opinion (for what it's worth). I think I'm probably correct in saying that here in the UK, the "wet" method is used in almost every lab in the country, if not all of the labs in the country. Nick Kirk Head Biomedical Scientist Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DMBCMP@aol.com Sent: 21 January 2004 03:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding W/WO Melted Paraffin Hi, everyone: In order to settle a difference of opinion between generations of trained Histotechs in my lab, may I have feedback from anyone interested in responding? Do you cover the blocks in melted paraffin in the embedding centre reservior and hold them that way while embedding? or.... Do you dump them in the warm reservior "dry" (not covered in melted paraffin) and embed them that way? Do you consider this "dry" method as bad technique since a tiny biopsy specimen MAY not be noticed as the paraffin quickly solidifies? We have a dispute. I have researched every book I can find and there is no reference to it anywhere. A newly trained tech that came to work for us said no mention was made during her training period. Some techs did...and some didn't. As a tech of nearly 27 years, I find this practice to be just asking for trouble. I was trained to keep everything melted. There seems to be some argument against keeping the cassettes in the melted paraffin, claiming it "cooks" the biopsies. I don't buy it, but what are the opinions of others? It seems so basic to me. I hope this does not come across as frivilous. Thanks for your input. Dannie Blake HT(ASCP) Histology Lead Tech Fresno Community Medical Centre Fresno, California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From powell_sa <@t> Mercer.EDU Wed Jan 21 13:42:15 2004 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Block Pulling Fees References: Message-ID: <005d01c3e056$ac18c6a0$e3f2acd1@powellsa1> Wow, I could be a millionaire. :) ----- Original Message ----- From: "Denise Bland-Piontek" To: Sent: Wednesday, January 21, 2004 11:58 AM Subject: [Histonet] Block Pulling Fees > Just wondering how many clinical labs bill for pulling blocks? Our > clinical lab just began charging $2.00 per block. Even our pathologists > must pay when having blocks pulled for research or education. Just > wondering if this is standard? Thanks, > Denise Bland-Piontek, HTL(ASCP) > Tissue Bank & Research Administrator > WVU > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 21 13:52:39 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding w/wo paraffin Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FC07@UTHEVS3.mail.uthouston.edu> I do not like the concept of placing tissues in a dry, heated area, before placing them in paraffin wax in a mold. I prefer to have a small container with molten paraffin wax and place tissues from there directly into the final mold. If tissues are placed "dry" there is greater possibility of paraffin wax draining from some areas and air bubbles developing. There was some mention about tissue carryover or losing tissue in the molten wax if you use this method. If the tissues are that small I think that it would be prudent to stain them with dilute eosin while processing so that they are clearly visible. Barry From Dndsomi <@t> aol.com Wed Jan 21 13:59:14 2004 From: Dndsomi <@t> aol.com (Dndsomi@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] GMS Staining Message-ID: We're having problems with our tissue, both patient and control, washing off during GMS staining. Has anyone else had this problem and if so how did you remedy it? From mcauliff <@t> umdnj.edu Wed Jan 21 13:59:19 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] embarrassing question In-Reply-To: References: Message-ID: <400EDA17.9040304@umdnj.edu> HI Sharon: In my experience the damage, if any, will depend on the tissue (some are more sensitive than others) and how good a look you get at it. Morphology that may be fine for the light microscope may not be fine for the EM. Cheer up, everyone I know has done this at least once! Geoff Sharon Cooperman wrote: > We recently had someone perfuse mice with 0.2M Sorensen's PBS instead > of 0.1M (with 4% formalin). I would expect the cells to shrivel, but > when I looked at H&E's of the sections they didn't look any worse than > some of our other blocks which were done correctly. Does anyone know > what happens when you perfuse tissues with 2x Sorensen's PBS or have > experience with this problem to tell me what problems I should look > out for - eg. damage to the tissues that might not be obvious on a > quick scan of an H&E? > > Thanks, > Sharon -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jqb7 <@t> cdc.gov Wed Jan 21 14:03:45 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] GMS Staining Message-ID: What type of slides do you use? And do you use a microwave method or a traditional method? Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dndsomi@aol.com Sent: Wednesday, January 21, 2004 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS Staining We're having problems with our tissue, both patient and control, washing off during GMS staining. Has anyone else had this problem and if so how did you remedy it? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ALee <@t> mrl.ubc.ca Wed Jan 21 14:05:40 2004 From: ALee <@t> mrl.ubc.ca (Albert Lee) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Mounting whole organs in wax Message-ID: Hi, Anybody have ideas on how to mount / impregnate whole organs in wax for display? Has anybody put large tissues on the processor for use in displays? Thanks! Albert Albert Lee, BSc., RT (CSLT) Cardiovascular Registry, the iCAPTURE Centre St. Paul's Hospital Room 166, Burrard Building 1081 Burrard Street, Vancouver, BC Canada V6Z 1Y6 Phone: 604 - 682 - 2344 extension 63572 Fax: 604 - 806 - 8158 From ahorstma <@t> waldenu.edu Wed Jan 21 14:17:30 2004 From: ahorstma <@t> waldenu.edu (Anne M. Horstmann) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] GMS Staining References: Message-ID: <000f01c3e05b$9c093240$492df7a5@anne> First check the type of slides you are using, then maybe look into Stay-on from Suripath. You add about a capful to the waterbath and this helps sections stay on slides. There is no background staining with Stay-on ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 2:59 PM Subject: [Histonet] GMS Staining > We're having problems with our tissue, both patient and control, washing off > during GMS staining. Has anyone else had this problem and if so how did you > remedy it? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dndsomi <@t> aol.com Wed Jan 21 14:36:51 2004 From: Dndsomi <@t> aol.com (Dndsomi@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] GMS Staining - P.S. Message-ID: Sorry, I should have mentioned that we are using charged slides. From paw555 <@t> yahoo.com Wed Jan 21 14:44:42 2004 From: paw555 <@t> yahoo.com (pam plumlee) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Another embedding query Message-ID: <20040121204442.40906.qmail@web11601.mail.yahoo.com> Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus From JCarpenter764 <@t> aol.com Wed Jan 21 14:48:13 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Question re: High School and 2 years experience not accepted a... Message-ID: i might be wrong but i am currently in the process of doing this myself and i believe that you have to have completed 2 years experience plus your high school diploma..Jennell From RossS <@t> BaylorHealth.edu Wed Jan 21 14:58:21 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] GMS Staining - P.S. Message-ID: You may have a bad batch of charged slides. This is too common. In my opinion you should not be able to float a section back off of a charged slide. Unfortunately far too often you can. I'm sure you have checked your oven temperature. At my former lab we had trouble with immuno tissue coming off the slides. We tried everything including extending the time in the oven, and changing brands and types of adhesive slides. Finally someone suggested leaving the slides to air dry upright for a couple of hours before putting them in the oven. The theory was that if water was under the tissue and the slides were put in the oven the water wouldn't really escape because the edges of the paraffin section would melt first trapping the water and causing the tissue to be more likely to lift off of the slide. I don't know how true that is, but we tried it and it worked. We left our immuno's to sit for 2 hours before putting them in the oven (forced air) at 60 C for 30 minutes. Most tissue that wasn't badly processed would then survive even the more extreme unmasking solutions. It is worth a try if you can't find another reason for your problem. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dndsomi@aol.com Sent: Wednesday, January 21, 2004 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS Staining - P.S. Sorry, I should have mentioned that we are using charged slides. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peptolab <@t> hamptons.com Wed Jan 21 15:29:37 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Artisan vs ????? Message-ID: <002301c3e065$ad5fc7c0$95a5bd18@JEFF> Hi Meredith. Why are you abandoning the Artisan (its Dako Cytomation now, not Cytologix). Is it the volume of your lab- are you unhappy with the Artisan. I have one for three years and I love it. Jeff Silverman Southside Hospital From JLE <@t> rice.willmar.mn.us Wed Jan 21 15:23:48 2004 From: JLE <@t> rice.willmar.mn.us (Jennifer Englin) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] alcian yellow on the nexes Message-ID: We are having difficulty getting even staining with our Alcian yellow on nexes. Our blue is very light and the yellow will start out dark on the top of the slide and fade gradually to faint or almost no staining by the end of the slide. We have tried both regular slides with gelatin and + slides without and it doesn't seem to make a difference. The module is level. If anyone has this problem or has a solution please let me know. Thanks, Jennifer Englin HT(ASCP),PA Rice Memorial Hospital Willmar MN jle@rice.willmar.mn.us From RossS <@t> BaylorHealth.edu Wed Jan 21 15:11:41 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Another embedding query Message-ID: At my former hospital they embedded dry with room temperature molds. Here they embed dry with hot molds. I think the practice of using room temperature molds at my former job was mostly due to the main embedding tech's preference for ergonomic comfort. They have the old Shandon Embedding Centers and she prefered the molds to be on top of the unit, reach for the tissue with the right hand and the mold with the left. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Wednesday, January 21, 2004 2:45 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Another embedding query Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Wed Jan 21 15:32:21 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Pen Message-ID: I wanted to share a wonderful discovery with everyone. We have had trouble finding pens that stay on cassette during processing and on slide during staining. We found a pen that works but it dulled rather quickly. We have found a pen that works great. I got it from Ted Pella and it is the RediSharp Plus. Hope this helps anyone that is having the same trouble as us. Becky From pruegg <@t> colobio.com Wed Jan 21 15:40:46 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] necrosis IHC markers In-Reply-To: Message-ID: some may not like this response but during my many hours of working on apoptosis using Tunnel assays we found it to be a very good marker for necrotic cells as a matter of fact it was not much use to us because we could not distinquish between apoptotic cells and necrotic cells. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of lynsay jackson Sent: Wednesday, January 21, 2004 7:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] necrosis IHC markers Hi, Does any body know any good immunohistochemistry markers for necrotic/dead cells? _________________________________________________________________ Tired of 56k? Get a FREE BT Broadband connection http://www.msn.co.uk/specials/btbroadband _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Wed Jan 21 15:35:34 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Recutting Old GMA tissue blocks In-Reply-To: Message-ID: cut GMA blocks off any block holders and infiltrate them in fresh activated monomer (A+C if using the JB4 kit) as if they are unembedded tissue as you got it in the first place. The GMA will penetrate through the polymerized block. Reembedd the reinfiltrated old block of tissue as for the first embedding. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barbara Harris Sent: Wednesday, January 21, 2004 10:40 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Recutting Old GMA tissue blocks Need helpful tips,protocols,and experienced advise, on how to resoften or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will be sectioning thin section for teaching purposes.Thanks in advance for any and all responses. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 21 16:12:25 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Another embedding query Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FC0C@UTHEVS3.mail.uthouston.edu> Pam Shoot the co-worker, keep the molds. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Wednesday, January 21, 2004 2:45 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Another embedding query Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfish <@t> gladstone.ucsf.edu Wed Jan 21 16:15:47 2004 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Pen In-Reply-To: References: Message-ID: Yes!!! These are the best. I use them on my slides, too, and I have NEVER lost a label through alcohol and xylenes!!! You do have to be careful not to rub the label end of the cassette after it hits 70%, though, but once the cassettes have been in paraffin there is no problem. Take care, Jo Dee Fish ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 From Jackie.O'Connor <@t> abbott.com Wed Jan 21 16:38:55 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] necrosis IHC markers Message-ID: In doing studies on cytotoxins, we have determined the same. TUNEL will stain necrotic as well as our desired apoptotic cells. I like your answer, Patsy. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Patsy Ruegg" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2004 03:40 PM To: "lynsay jackson" , cc: Subject: RE: [Histonet] necrosis IHC markers some may not like this response but during my many hours of working on apoptosis using Tunnel assays we found it to be a very good marker for necrotic cells as a matter of fact it was not much use to us because we could not distinquish between apoptotic cells and necrotic cells. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of lynsay jackson Sent: Wednesday, January 21, 2004 7:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] necrosis IHC markers Hi, Does any body know any good immunohistochemistry markers for necrotic/dead cells? _________________________________________________________________ Tired of 56k? Get a FREE BT Broadband connection http://www.msn.co.uk/specials/btbroadband _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Jan 21 16:45:35 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] How to eliminate holes in mouse brain tissue? In-Reply-To: Message-ID: <3.0.6.32.20040121154535.00bd7798@gemini.msu.montana.edu> AMy, Freezing cryoprotected tissue in a cryostat will create freezing artifact (big holes caused by water ice crystal formation with lower temperature/slower freezing in a cryostat). You need to SNAP FREEZE. Histonet archives is filled with suggestions on various ways to snap freeze, do a search as this has been discussed at length recently. Sucrose helps reduce this problem, but it can still rear its ugly head with cryostat freezing. Be sure you cut at a correct temperature also, cryoprotected/prefixed tissue I find are cryosectioning well at around -26C otherwise the sucrose oozes out like Karo syrup. At 02:11 PM 1/21/2004 -0500, you wrote: >Hi, >I am writing to see if I can get some advice on how to eliminate holes I >am seeing in my tissue. >I am using tissue from the mouse olfactory bulb and brain for fos ICC. >Unfortunately I have been unable to see any fos because my tissue is full >of little holes and therefore I have no actual cells. I have determined >that it is not the ICC that is causing the holes because they are present >before staining. So I am assuming I am doing something wrong during >fixation and cryoprotection. I perfuse the animal with 4% para at a rate >of 3.5ml/min and I give the animal around 50mls. I then post fix with 4% >para overnight and then cryoprotect with 30% sucrose until the brain drops >(usually overnight). The brain is then imbedded in OCT and put in the >cryostat at -20C to freeze and cut at 20microns. I typically do not >perfuse with PBS before para but others in my lab have tried perfusing >with and without PBS and have found no difference in tissue quality. Any >suggestions as to how I can fix this? > >Thanks, > Amy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Jackie.O'Connor <@t> abbott.com Wed Jan 21 16:31:24 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Another embedding query Message-ID: As long as you have a homogenous block with all your tissue at the appropriate plane - I don't think it much matters. If you thrust cold tissue into molten paraffin, you're going to have tissue popping out because of the lack of homogenous(ness?) Homogeneity? You fill in the noun. If your mold is too cold when you put your tissue in, your block will crumble around the edges. That's why we are artists - each block is unique, and takes a skilled person with some brains (left after formalin fixation) to determine what is best for each block. I've tried everything - that's how I learned what NOT to do. I prefer using room temp molds because some of the newer cassettes have a perfect leak-hole at the bottom where the hinge was - if the mold is as hot as the paraffin, it leaks a lot. If the mold is room temp, it chills faster, and I have less leaking paraffin. Face it - it's a messy job. If we're taking a poll, I keep my cassettes in the warm embedding center sans molten paraffin - but I embed everything in less than an hour. I find for my purposes, the tissue is kept at a decent temp to embed, and the paraffin it was processed in protects it from the air. Seems to work OK for what I'm doing. If it ain't broke, don't fix it. I'm all for heading off disaster, but I don't see any looming on the horizon for us as far as embedding artifacts, etc. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Stapf, Ross" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2004 03:11 PM To: "pam plumlee" , cc: Subject: RE: [Histonet] Another embedding query At my former hospital they embedded dry with room temperature molds. Here they embed dry with hot molds. I think the practice of using room temperature molds at my former job was mostly due to the main embedding tech's preference for ergonomic comfort. They have the old Shandon Embedding Centers and she prefered the molds to be on top of the unit, reach for the tissue with the right hand and the mold with the left. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Wednesday, January 21, 2004 2:45 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Another embedding query Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Wed Jan 21 17:16:47 2004 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Another embedding query Message-ID: <8C6E05FA69571948B461F1327CBB893E16C345@LMMAIL2> I agree with Jackie... We embed almost exactly as Jackie and we've done so for years and years. I've been here for 13 years and we embedded the same at the hospital I worked at before I came here. Never had any problems. Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Wednesday, January 21, 2004 4:31 PM To: Stapf, Ross Cc: histonet@pathology.swmed.edu Subject: RE: [Histonet] Another embedding query As long as you have a homogenous block with all your tissue at the appropriate plane - I don't think it much matters. If you thrust cold tissue into molten paraffin, you're going to have tissue popping out because of the lack of homogenous(ness?) Homogeneity? You fill in the noun. If your mold is too cold when you put your tissue in, your block will crumble around the edges. That's why we are artists - each block is unique, and takes a skilled person with some brains (left after formalin fixation) to determine what is best for each block. I've tried everything - that's how I learned what NOT to do. I prefer using room temp molds because some of the newer cassettes have a perfect leak-hole at the bottom where the hinge was - if the mold is as hot as the paraffin, it leaks a lot. If the mold is room temp, it chills faster, and I have less leaking paraffin. Face it - it's a messy job. If we're taking a poll, I keep my cassettes in the warm embedding center sans molten paraffin - but I embed everything in less than an hour. I find for my purposes, the tissue is kept at a decent temp to embed, and the paraffin it was processed in protects it from the air. Seems to work OK for what I'm doing. If it ain't broke, don't fix it. I'm all for heading off disaster, but I don't see any looming on the horizon for us as far as embedding artifacts, etc. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Stapf, Ross" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2004 03:11 PM To: "pam plumlee" , cc: Subject: RE: [Histonet] Another embedding query At my former hospital they embedded dry with room temperature molds. Here they embed dry with hot molds. I think the practice of using room temperature molds at my former job was mostly due to the main embedding tech's preference for ergonomic comfort. They have the old Shandon Embedding Centers and she prefered the molds to be on top of the unit, reach for the tissue with the right hand and the mold with the left. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Wednesday, January 21, 2004 2:45 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Another embedding query Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From stancelb <@t> msn.com Wed Jan 21 17:17:45 2004 From: stancelb <@t> msn.com (Barbara Stancel) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Sakura or Leica Cassette Printer Message-ID: Laurie, We just bought the Sakura cassette and slide autowriters. Love'em. Love'em. Love'em. The only difficult part was training the techs......it took us quite a while to catch on. Me longer than the others. But once we caught on, it has been great. Great service from Sakura also (thanks Sharon Weyman for your hours and hours of patience!). Histologically yours, Barbara USDA, FSIS, OPHS, Eastern Laboratory, Pathology Athens, Georgia 30604 Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, January 21, 2004 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Cassette Printer Does anyone out there have or tried out the Leica IP C Cassetter Printer? If so, can you provide any feedback? I am still trying to find a cassette labeler that will work for us. We have tried the Shandon, the TBS, and the new Sakura, but I wasn't totally happy with any of them. Does anyone have info on the annual CSH meeting this year? Laurie Colbert Huntington Hospital Pasadena, CA _________________________________________________________________ There are now three new levels of MSN Hotmail Extra Storage! Learn more. http://join.msn.com/?pgmarket=en-us&page=hotmail/es2&ST=1 From Boneslides <@t> aol.com Wed Jan 21 17:20:48 2004 From: Boneslides <@t> aol.com (Boneslides@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Cole's Hematoxylin Message-ID: Does anyone have a procedure for making Cole's Hematoxylin that they would be willing to share?? Thanks in advance! Diane Mahovlic, HT(ASCP) Orthopedic Pathology and Biomaterials Laboratory The Cleveland Clinic Foundation Cleveland, Ohio From peptolab <@t> hamptons.com Wed Jan 21 17:39:58 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding technique Message-ID: <000e01c3e078$1b744d50$95a5bd18@JEFF> I use room temperature molds for everything (Tissue Tek Plastics). The only things that get melted on the warm plate are any needle biopsies- melt the bottom layer, place em in and position, move to cold plate and quickly tamp with the little tamper thingies. The larger sized rectangular molds are banned from my lab. I embed from the holding reservoir filled half way or less. Everything sinks to the bottom and comes out fine, the relative chill though enables most curettings to come out in one cake- easy to transfer to the mold. Tricks: TURP, I gross in a "monolayer" of prostate chips in the cassette. When embedding, I invert the cassette over the base mold and tap, everything falls into place (if your lucky) and there is no need to mess with the chips further. Gunk wrapped in lens tissue can be cooled ever so slightly, scraped off the paper with a slide, as the layer of wax and gunk builds up on the edge, one can warm the bottom of the slide gently and scrape it off in one piece right into the mold. One thing though- I sure miss my bunsen burner. It would keep us warm during these frigid winter days. Jeff Silverman Southside Hospital Bay Shore NY From peptolab <@t> hamptons.com Wed Jan 21 17:43:30 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding technique Message-ID: <001301c3e078$6376c010$95a5bd18@JEFF> I use room temperature molds for everything (Tissue Tek Plastics). They are filled leaving 0.5-1 mm empty at the top. Saves lots of time cleaning the blocks and everything is level and fine at the bottom. The only things that get melted on the warm plate are any needle biopsies- melt the bottom layer, place em in and position, move to cold plate and quickly tamp with the little tamper thingies. The larger sized rectangular molds are banned from my lab. I embed from the holding reservoir filled half way or less. Everything sinks to the bottom and comes out fine, the relative chill though enables most curettings to come out in one cake- easy to transfer to the mold. Tricks: TURP, I gross in a "monolayer" of prostate chips in the cassette. When embedding, I invert the cassette over the base mold and tap, everything falls into place (if your lucky) and there is no need to mess with the chips further. Gunk wrapped in lens tissue can be cooled ever so slightly, scraped off the paper with a slide, as the layer of wax and gunk builds up on the edge, one can warm the bottom of the slide gently and scrape it off in one piece right into the mold. One thing though- I sure miss my bunsen burner. It would keep us warm during these frigid winter days. Jeff Silverman Southside Hospital Bay Shore NY From bhewlett <@t> cogeco.ca Wed Jan 21 19:51:54 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] CD13 -- FFPE immunohistochemistry References: <3.0.6.32.20040121113157.0114b2e0@137.73.66.5> Message-ID: <000401c3e08a$50179fa0$6500a8c0@bryanmainbox> Alex, I haven't tried it, but Novocastra list the following; NCL-CD13-304, clone 38C12, which is reported to work on FFPE following HIER in citrate buffer pH6.0 Worth a try! Regards, Bryan ----- Original Message ----- From: "Alex Knisely" To: "HistoNet Server" Sent: Wednesday, January 21, 2004 6:31 AM Subject: [Histonet] CD13 -- FFPE immunohistochemistry > CD13, also called "aminopeptidase N", is a white-cell antigen usually > assessed by flow cytometry. > > I'd like to look at it in FFPE materials. > > A search through ABCAM has not found a reference to a commercially > available source of an anti-CD13 antibody that works in FFPE sections. > > Can anyone recommend a source and protocol for such an antibody? > > Best thanks in advance > > Alex K > > > Alex Knisely, MD > Consultant Histopathologist > > alex.knisely@kcl.ac.uk > > Institute of Liver Studies > King's College Hospital > Denmark Hill > London SE5 9RS UK > > +44 (0)20 - 7346 - 3125 telefax > +44 (0)20 - 7346 - 4627 office > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Wed Jan 21 20:03:28 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Cole's Hematoxylin References: Message-ID: <001501c3e08b$edb2f470$6500a8c0@bryanmainbox> Diane, Go to the following for everything you need; http://members.pgonline.com/~bryand/StainsFile/stain/hemalum/cole.htm Regards, Bryan ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 6:20 PM Subject: [Histonet] Cole's Hematoxylin > Does anyone have a procedure for making Cole's Hematoxylin that they would be > willing to share?? > > Thanks in advance! > Diane Mahovlic, HT(ASCP) > Orthopedic Pathology and Biomaterials Laboratory > The Cleveland Clinic Foundation > Cleveland, Ohio > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WWmn916 <@t> aol.com Wed Jan 21 20:26:45 2004 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] HT job opportunity, Sacramento Message-ID: <192.24f5498e.2d408ee5@aol.com> We are looking for qualified histotechs (ASCP certified, but will consider registry eligible and experienced). We are located in Sacramento, CA. We offer great benefits and hard to beat profit sharing. Yosemite, the ocean, Lake Tahoe, tall redwoods, are geographical attractions in this area. We are a busy laboratory and growing! For more information please call (916) 447-2718. Deborah King, HT (ASCP) Email: WWmn916@aol.com From bryand <@t> netbistro.com Wed Jan 21 20:39:48 2004 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Cole's Hematoxylin References: Message-ID: <019601c3e091$036c1300$a370c2cf@bryand> The formula for Cole's hemalum is on StainsFile; http://stainsfile.info. Choose staining techniques, then mordanted hematoxylin formulae. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 3:20 PM Subject: [Histonet] Cole's Hematoxylin > Does anyone have a procedure for making Cole's Hematoxylin that they would be > willing to share?? > > Thanks in advance! > Diane Mahovlic, HT(ASCP) > Orthopedic Pathology and Biomaterials Laboratory > The Cleveland Clinic Foundation > Cleveland, Ohio > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From sonyalhogg <@t> yahoo.co.nz Thu Jan 22 00:40:14 2004 From: sonyalhogg <@t> yahoo.co.nz (=?iso-8859-1?q?Sonya=20Hogg?=) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Website help Message-ID: <20040122064014.13637.qmail@web14903.mail.yahoo.com> I am looking for websites relating to histology special staining techniques and am having difficulty does anyone have any good links?? Thanks http://personals.yahoo.com.au - Yahoo! Personals New people, new possibilities. FREE for a limited time. From gagermeierjp <@t> upmc.edu Thu Jan 22 00:56:43 2004 From: gagermeierjp <@t> upmc.edu (Gagermeier, James) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] (no subject) Message-ID: <9122C182D4268F45BCB9E64A10B7331F024D1247@1upmc-msx7.isdip.upmc.edu> Basics of LCM A few questions in regards to laser capture microdissection utilizing a Leica AS LMD System: In regards to buffers to place in the caps - I have routinely used RNA later. This, however, readily crystalizes setting up issues with debris on the microscope. First, is this an acceptable buffer, and second, what are some other buffer alternatives. Furthermore, when preparing slides, I generally store them in a -80 C unit. I generally place them in a zip lock bag - not a heat sealed bag. Also, I do not generally add a dessicant. Should: a) I use heat-sealed bags. If not, do I need to place them in a zip lock at all ?(i.e. can I simply put them in a slide box in the -80C unit) b) Do I need to start using a dessicant? I appreciate any response on these issues. Jim Gagermeier From nick.kirk3 <@t> btopenworld.com Thu Jan 22 01:33:38 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Website help In-Reply-To: <20040122064014.13637.qmail@web14903.mail.yahoo.com> Message-ID: Try http://members.pgonline.com/~bryand/StainsFile/ or http://www.nottingham.ac.uk/pathology/default.html or http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sonya Hogg Sent: 22 January 2004 06:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Website help I am looking for websites relating to histology special staining techniques and am having difficulty does anyone have any good links?? Thanks http://personals.yahoo.com.au - Yahoo! Personals New people, new possibilities. FREE for a limited time. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From G.A.McHardy <@t> arh.grampian.scot.nhs.uk Thu Jan 22 02:46:46 2004 From: G.A.McHardy <@t> arh.grampian.scot.nhs.uk (G.A.McHardy@arh.grampian.scot.nhs.uk) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] RE: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS Anti-Virus] Message-ID: Does anyone know of a relatively safe, commercially available substitute for acid dichromate glassware cleaning solution? In our neuropathology lab we prepare this solution in-house and it is used for general glassware cleaning and especially for glassware used in silver staining methods. There are some commercial reagents available in the US but I have yet to source one in the UK. Any ideas? Graham A McHardy Pathology Dept ARI Aberdeen > -----Original Message----- > From: histonet-request@lists.utsouthwestern.edu > [SMTP:histonet-request@lists.utsouthwestern.edu] > Sent: 21 January 2004 18:00 > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS > Anti-Virus] > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: apotosis marker in mice (Jackie.O'Connor@abbott.com) > 2. Re: Working Saturdays (Gareth Davis) > 3. Hep C (Luis Chiriboga) > 4. RE: HT Certified vs. HT non-certified (Morken, Tim - Labvision) > 5. Recutting Old GMA tissue blocks (Barbara Harris) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Jan 2004 11:25:43 -0600 > From: Jackie.O'Connor@abbott.com > Subject: Re: [Histonet] apotosis marker in mice > To: Susan Q Wells > Cc: histonet-bounces@lists.utsouthwestern.edu, > histonet@pathology.swmed.edu > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > I use Caspase-3 routinely for apoptosis. I use an antibody on zinc fixed > paraffin embedded tissues, but there are some out there for FFPE. > Much easier than TUNEL, and more specific. > > > > > Susan Q Wells > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/21/2004 10:17 AM > > > To: histonet@pathology.swmed.edu > cc: > Subject: [Histonet] apotosis marker in mice > > > Hello fellow histonetters - Does anyone have a straightforward > protocol for an apotosis marker to use on frozen or paraffin mice > sections.I used the TUNEL stain a number of years ago but I'm > thinking there may be an antibody out there that works just as > well? > Thanks in advance for your time, > Sue Wells HT(ASCP),QIHC > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Wed, 21 Jan 2004 09:28:28 -0800 (PST) > From: Gareth Davis > Subject: Re: [Histonet] Working Saturdays > To: "Weems, Joyce" , Histonet > > Message-ID: <20040121172828.37965.qmail@web13306.mail.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > In our lab at PathGroup, the day shift takes turns to do Saturdays. In > our case we only need one person, because don't do any special stains or > IHC. We basically just cut and get slides ready to be done early Monday > moring. But, the person who is scheduled to work on a Saturday gets the > previous Monday (so that the time works). > Good luck. > > Gareth Blaeuer Davis > Histotechinican > PathGroup > Nashville, Tennessee > > "Weems, Joyce" wrote: > > An unofficial survey: > > How many hospitals are working Saturdays? And what is the case load of > those > that do? > > Thanks for your help! > > Joyce > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph?EUR(tm)s Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------- > Do you Yahoo!? > Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes > > ------------------------------ > > Message: 3 > Date: Wed, 21 Jan 2004 12:34:12 -0500 > From: Luis Chiriboga > Subject: [Histonet] Hep C > To: Histonet > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi All > is anyone using/trying/ have an antibody for Hep C > Thanks > Luis > > ------------------------------ > > Message: 4 > Date: Wed, 21 Jan 2004 09:34:45 -0800 > From: "Morken, Tim - Labvision" > Subject: RE: [Histonet] HT Certified vs. HT non-certified > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <0556BE8AC5551E4E8AF6BB9E42509BA2035762@usca0082k08.labvision.apogent.com> > > Content-Type: text/plain > > Yes, the age old question. As usual, it depends...... > > I worked with an uncertified histotech of over 15 years experience who > could > do the usual special stains well, and was an excellet cutter, but who > could > not seem to develop methods on his own, write any meaningful procedures or > even read very well. Basically he was one of those with one year of > experience repeated 15 times. Although he was not certified it was not > for > lack of trying - he tried five times and couldn't pass the written test > (which goes along with the inability to develop procedures, I think, and > he > only attempted certification when others in the lab started a study > group). > But he had some skill so he was an asset to the lab as a general bench > worker. > > I have also worked with formally-trained (ie, completed a course), > certified, very interested, but who were simply mediocre and never > advanced. > > > Then there are those OJT trained techs who got certified by their own hard > work and were absolutely excellent and advanced almost at will (Indeed, I > recently interviewed a non-certified histotech of only two years of > experience (and no formal histotech courses) who had accomplished more in > that two years than most techs do in their entire careers!). > > So, it depends on the person. I tend to be concerned about a person who > has > worked in the field for 15 years and is not certified. That is due either > to > lack of interest, or lack of ability in some way. A person who has > received > the certificaiton has proven their basic skill AND at least some potential > for personal advancement. After all, the test is a personal endeavor; the > person has to do the lab work, the reading, and the test. You don't get > certified just because you occupied a space in the lab for 15 years. And > if > the issue is trainability, probably the certified person is going to be > more > trainiable than someone who can't, or won't attempt to, get certified > > So I guess the short answer is to look into their past very carefully and > see what they have been doing. Never base it simply on years (long or > short) > or certification alone. > > Tim Morken > > > -----Original Message----- > From: Histolady710@aol.com [mailto:Histolady710@aol.com] > Sent: Tuesday, January 20, 2004 4:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HT Certified vs. HT non-certified > > > This is for histology managers ! I'm curious as to which HT you would > hire > - > a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years > experience. Thanks. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 21 Jan 2004 11:39:48 -0600 > From: "Barbara Harris" > Subject: [Histonet] Recutting Old GMA tissue blocks > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Need helpful tips,protocols,and experienced advise, on how to resoften > or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will > be sectioning thin section for teaching purposes.Thanks in advance for > any and all responses. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 2, Issue 29 > *************************************** From ames1 <@t> breathe.com Thu Jan 22 03:20:03 2004 From: ames1 <@t> breathe.com (ames1@breathe.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Rat Micronuclei scoring In-Reply-To: References: Message-ID: Hi there, I'm hoping someone can help me with a staining technique to view micronuclei in PCEs and NCEs on rat bone marrow smears. Apparently the best technique is the H&E stain but when I try it the haematoxylin is too strong. I have tried reducing the concentration to 0.5% but the some of the PCEs are still blacked out. Am I doing something wrong? I have resolved the Eosin being overpowering by using aqueous Eosin. I hope everyone is enjoying the New Year. My thanks to anyone who can help, Amy Greenhalgh Scientist Sequani Ltd, Bromyard Road, Ledbury, HR8 1LH From lynzjackson <@t> hotmail.com Thu Jan 22 04:00:57 2004 From: lynzjackson <@t> hotmail.com (lynsay jackson) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] apoptosis/necrosis IHC antibodies Message-ID: Hi does anyone know any companies to buy IHC necrosis/apoptosis antibodies from? _________________________________________________________________ Express yourself with cool emoticons - download MSN Messenger today! http://www.msn.co.uk/messenger From gudrun.lang <@t> aon.at Thu Jan 22 05:04:18 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Embedding W/WO Melted Paraffin References: Message-ID: <005d01c3e0d7$7b5bf380$eeeea8c0@SERVER> In my lab we do the "dry" method for at least twenty years and have no problems. The probes stay in the 60 degree-oven and then in the warm area of the embedding center for about two hours, while embedding. It is cleaner to handle. Lang Gudrun ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 4:37 AM Subject: [Histonet] Embedding W/WO Melted Paraffin > Hi, everyone: > In order to settle a difference of opinion between generations of trained > Histotechs in my lab, may I have feedback from anyone interested in responding? > Do you cover the blocks in melted paraffin in the embedding centre reservior > and hold them that way while embedding? or.... > Do you dump them in the warm reservior "dry" (not covered in melted paraffin) > and embed them that way? > Do you consider this "dry" method as bad technique since a tiny biopsy > specimen MAY not be noticed as the paraffin quickly solidifies? > We have a dispute. I have researched every book I can find and there is no > reference to it anywhere. A newly trained tech that came to work for us said > no mention was made during her training period. Some techs did...and some > didn't. As a tech of nearly 27 years, I find this practice to be just asking for > trouble. I was trained to keep everything melted. There seems to be some > argument against keeping the cassettes in the melted paraffin, claiming it "cooks" > the biopsies. I don't buy it, but what are the opinions of others? > It seems so basic to me. I hope this does not come across as frivilous. > Thanks for your input. > > Dannie Blake HT(ASCP) Histology Lead Tech > Fresno Community Medical Centre > Fresno, California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From abright <@t> brightinstruments.com Thu Jan 22 05:52:02 2004 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] peltier vs. refrigeration cold stage for slidingmicrotome Message-ID: Dear Kathy, We manufacture both systems the two Peltier solid state type, have cooling outputs for a 30 X 30mm stage is 15watts @ 0?C & 40 X 40mm stage is 25 watts @ 0?C. The refrigerated Stage type cooling output for a 90 x 130mm stage is 300 watts @ 0?C decreasing to 125 watts @ -30?C. With this information you will need to decide which suits the sizes and sectioning temperature of the specimens required for sectioning best, If you require any additional assistance, I will be only to willing to help. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: katherine-walters@uiowa.edu [mailto:katherine-walters@uiowa.edu] Sent: 21 January 2004 14:27 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peltier vs. refrigeration cold stage for slidingmicrotome Dear Histologists, I am beginning the process of looking at cold stages for a sliding microtome. Does anyone have experience or opinions about the usage of peltier stages as opposed to refrigeration stages? I would also appreciate any information on which companies have these for sale. Thank you once again, Kathy Walters _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mparker <@t> epl-inc.com Thu Jan 22 05:53:50 2004 From: mparker <@t> epl-inc.com (Mary Parker) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Fw: Shur/Mount Coverslipper Message-ID: <007c01c3e0de$67955fb0$6b01a8c0@93F1H11> ----- Original Message ----- From: Mary Parker To: histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 22, 2004 6:45 AM Subject: Shur/Mount Coverslipper If anyone has a newer model of this automatic coverslipper (formerly Hacker, now TBS), please tell me which mounting medium has worked best. I understand from the sales rep that we can not use Permount on this machine. Any comments? Mary Parker, H.T., A.S.C.P. Histology Manager EPL, Inc. P.O. Box 12766 RTP, N.C. 27709 (919) 998-9407 From MAUGER <@t> email.chop.edu Thu Jan 22 06:52:35 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Fw: Shur/Mount Coverslipper Message-ID: Mary, In reference to the mounting medium for the Hacker- Permount is toluene based. If you use xylene as a clearing agent, you should use a xylene based mounting medium. The toluene based one will dry out real quickly, and you will have coverslips popping off,and lots of extra work. Jo >>> "Mary Parker" 01/22/04 06:53AM >>> ----- Original Message ----- From: Mary Parker To: histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 22, 2004 6:45 AM Subject: Shur/Mount Coverslipper If anyone has a newer model of this automatic coverslipper (formerly Hacker, now TBS), please tell me which mounting medium has worked best. I understand from the sales rep that we can not use Permount on this machine. Any comments? Mary Parker, H.T., A.S.C.P. Histology Manager EPL, Inc. P.O. Box 12766 RTP, N.C. 27709 (919) 998-9407 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Cderiso <@t> harthosp.org Thu Jan 22 07:16:16 2004 From: Cderiso <@t> harthosp.org (Cindy Deriso) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Block Pulling Fees Message-ID: And why not.. I would rather charge at the other end. Filing... at least $5 per slide and 3 per block! >>> "Denise Bland-Piontek" 01/21/04 11:58AM >>> Just wondering how many clinical labs bill for pulling blocks? Our clinical lab just began charging $2.00 per block. Even our pathologists must pay when having blocks pulled for research or education. Just wondering if this is standard? Thanks, Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator WVU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Jan 22 07:26:35 2004 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] Pen Message-ID: <3D502BBF5356D31184650090275B750D0346C6C8@mail.cooley-dickinson.org> We use these pens. I tried to reorder and was told that they were no longer being manufactured. Do you mind sharing your vendor info? Thanks, Stacy -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Wednesday, January 21, 2004 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pen I wanted to share a wonderful discovery with everyone. We have had trouble finding pens that stay on cassette during processing and on slide during staining. We found a pen that works but it dulled rather quickly. We have found a pen that works great. I got it from Ted Pella and it is the RediSharp Plus. Hope this helps anyone that is having the same trouble as us. Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From BSylinda <@t> aol.com Thu Jan 22 07:50:25 2004 From: BSylinda <@t> aol.com (BSylinda@aol.com) Date: Fri Sep 16 15:22:28 2005 Subject: [Histonet] pens Message-ID: <623063F4.6295A405.00698496@aol.com> We use a Statmark Pen from Statlab, that is resistant in alcohols and xylene. Statlab Medical Products 18004423573 item # SMP-BK Sylinda From Terry.Marshall <@t> rothgen.nhs.uk Thu Jan 22 07:22:47 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Block Pulling Fees Message-ID: If techs charge for pulling blocks, do ornithologists charge for pulling birds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From DPALLP <@t> aol.com Thu Jan 22 09:08:15 2004 From: DPALLP <@t> aol.com (DPALLP@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] coverslipper justification Message-ID: <34FA2027.1A9497BC.00042A59@aol.com> Is there a general rule about the volume of slides needed to justify the cost of an automated coverslipper? One of my pathologists seems to think the magical number is 300 slides per day. Thanks. Susie From jqb7 <@t> cdc.gov Thu Jan 22 09:17:39 2004 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] coverslipper justification Message-ID: I think that whenever you can walk away from a machine while it is performing a task and do other work, then that it is a productive and justified item. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DPALLP@aol.com Sent: Thursday, January 22, 2004 10:08 AM To: histonet@pathology.swmed.edu Subject: [Histonet] coverslipper justification Is there a general rule about the volume of slides needed to justify the cost of an automated coverslipper? One of my pathologists seems to think the magical number is 300 slides per day. Thanks. Susie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kspencer <@t> utmem.edu Thu Jan 22 09:36:09 2004 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] (no subject) In-Reply-To: <9122C182D4268F45BCB9E64A10B7331F024D1247@1upmc-msx7.isdip.upmc.edu> Message-ID: I put slides in a slide box with dessicant, wrap the box in foil, and place this in a ziploc bag. Then into the -80. I am sure you know never to let them air dry. I have an Arcturus LCM and we mostly use their kits, thus their buffers. Kathleen Spencer LCM Supervisor UTHSC On Thursday, January 22, 2004, at 12:56 AM, Gagermeier, James wrote: > Basics of LCM > > A few questions in regards to laser capture microdissection utilizing a > Leica AS LMD System: > > In regards to buffers to place in the caps - I have routinely used RNA > later. This, however, readily crystalizes setting up issues with debris > on > the microscope. First, is this an acceptable buffer, and second, what > are > some other buffer alternatives. > > Furthermore, when preparing slides, I generally store them in a -80 C > unit. > I generally place them in a zip lock bag - not a heat sealed bag. Also, > I do > not generally add a dessicant. Should: > > a) I use heat-sealed bags. If not, do I need to place them in a zip > lock at > all ?(i.e. can I simply put them in a slide box in the -80C unit) > > b) Do I need to start using a dessicant? > > I appreciate any response on these issues. > > > Jim Gagermeier > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahorstma <@t> waldenu.edu Thu Jan 22 09:44:32 2004 From: ahorstma <@t> waldenu.edu (Anne M. Horstmann) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] HT/HTL Job Offer Message-ID: <001d01c3e0fe$a3a32940$f41cf7a5@anne> Private DermPath Lab on Long Island is looking for a F/T Days (M-F) Histology Tech. At least an Associate degree and HT/HTL eligible required. Willing to train someone if they don't know how to gross Derm specimens. Please either e-mail ahorstma@waldenu.edu or call 516-944-7104 if interested. Thanks From powell_sa <@t> Mercer.EDU Thu Jan 22 09:53:37 2004 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Website help References: <20040122064014.13637.qmail@web14903.mail.yahoo.com> Message-ID: <001e01c3e0ff$e6158c40$e3f2acd1@powellsa1> Hi Sonya, Visit histosearch.com and check out the links on the home page. You will find the histonet archives at this site also. Shirley Powell www.histosearch.com ----- Original Message ----- From: "Sonya Hogg" To: Sent: Thursday, January 22, 2004 1:40 AM Subject: [Histonet] Website help > I am looking for websites relating to histology > special staining techniques and am having difficulty > does anyone have any good links?? Thanks > > http://personals.yahoo.com.au - Yahoo! Personals > New people, new possibilities. FREE for a limited time. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From L.D.Ridley <@t> bham.ac.uk Thu Jan 22 10:03:20 2004 From: L.D.Ridley <@t> bham.ac.uk (LD Ridley) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Tissue Microarrays. Message-ID: <1074787400.b15467e0L.D.Ridley@bham.ac.uk> Hi Histonetters. I am just seeing how many of you out there currently use tissue microarrays either routinely or for research. We are currently researching paediatric brain tumours and are about to get started on our first 'real' array using the Beecher's TMAinstrument using a normal positive control tissue block for which we have lots and lots of tissue. I am just seeing if anyone else has a lot of experience with this technique and would like to discuss any good tips they may have before we take the plunge with our 'precious' research material Thanks in advance Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 From Boneslides <@t> aol.com Thu Jan 22 10:02:51 2004 From: Boneslides <@t> aol.com (Boneslides@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Thank-you!! Message-ID: <157.2c21a827.2d414e2b@aol.com> Thanks to all the folks who were kind enough to respond to my request for a Cole's Hematoxylin recipe!!! I really appreciate the quick response!! Have a nice day!!!! Diane Mahovlic THe Cleveland Clinic Foundation Cleveland, Ohio From MTitford <@t> aol.com Thu Jan 22 10:13:57 2004 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Is "Chromopaque" still available? Message-ID: <0EEC395E.5A04BE27.00762DB1@aol.com> Many years ago, in a world far, far away (When I lived and worked in London in the 60's)we used a colored radio-opaque product for injection studies. It was quite good. Injected easily, X-ray'd well, and the tissues processed and sectioned well afterwards. Additionally the colors did not bleed out! Is this product still available here in the States or in Great Britain? Thank you in advance Mike Titford USA Pathology Mobile AL USA From kemlo <@t> tiscali.co.uk Thu Jan 22 10:24:15 2004 From: kemlo <@t> tiscali.co.uk (Kemlo Rogerson) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Block Pulling Fees In-Reply-To: Message-ID: <000701c3e104$3101b4a0$e2362850@KEMLOS> No but the birds would! Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 01270 877625 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts:? ???????????? kemlo@tiscali.co.uk?or kemlo1@btinternet.com? Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 22 January 2004 13:23 To: histonet@pathology.swmed.edu Subject: RE: [Histonet] Block Pulling Fees If techs charge for pulling blocks, do ornithologists charge for pulling birds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lizchlipala <@t> premierhistology.com Thu Jan 22 10:26:37 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Rat Micronuclei scoring In-Reply-To: Message-ID: <000001c3e104$855c8de0$74d48a80@LIZ> When we did this in the past we used a diff quick stain to stain the slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ames1@breathe.com Sent: Thursday, January 22, 2004 2:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat Micronuclei scoring Hi there, I'm hoping someone can help me with a staining technique to view micronuclei in PCEs and NCEs on rat bone marrow smears. Apparently the best technique is the H&E stain but when I try it the haematoxylin is too strong. I have tried reducing the concentration to 0.5% but the some of the PCEs are still blacked out. Am I doing something wrong? I have resolved the Eosin being overpowering by using aqueous Eosin. I hope everyone is enjoying the New Year. My thanks to anyone who can help, Amy Greenhalgh Scientist Sequani Ltd, Bromyard Road, Ledbury, HR8 1LH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From am102 <@t> st-andrews.ac.uk Thu Jan 22 11:02:34 2004 From: am102 <@t> st-andrews.ac.uk (Anne-Sophie Martinez) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] (no subject) Message-ID: <200401221652.QAA25491@bute.st-andrews.ac.uk> Hello everybody, Can I localize the intestinal cells which produce the serotonin, not with an antibody against this hormone, but with a special histological staining? Thank you very much for your help. AS ---------------------------------------------- Dr Anne-Sophie Martinez School of Biology University of St. Andrews Bute Medical Buildings St. Andrews Fife, KY16 9TS UK. Tel + 44 (0) 1334 463546 Fax + 44 (0) 1334 463600 From syedab <@t> totalise.co.uk Thu Jan 22 11:17:01 2004 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] DAB precipitates Help please References: <20040122064014.13637.qmail@web14903.mail.yahoo.com> <001e01c3e0ff$e6158c40$e3f2acd1@powellsa1> Message-ID: <001401c3e10b$8dcfc6c0$80c401a3@clneuro.ox.ac.uk> Dear All, I have been using DAB successfully for a while, but today when I stained my sections, there were huge clumps of DAB precipitates stuck all over the section. I didn't do anything different and it was a brand new kit. I am sure that I mixed it well as I vortex for about ten seconds prior to use. Is there a way of removing these to see the section underneath? I have tried copious washing with PBS and left them for a long time in alcohols and histoclear. All help gratefully appreciated, Anila Syed --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.561 / Virus Database: 353 - Release Date: 13/01/04 From gudrun.lang <@t> aon.at Thu Jan 22 12:21:10 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Block Pulling Fees References: Message-ID: <003401c3e114$829cde60$eeeea8c0@SERVER> Please, what is the meaning of "pulling blocks"? An unknowing Austrian histotech ----- Original Message ----- From: "Marshall Terry Dr,Consultant Histopathologist" To: Sent: Thursday, January 22, 2004 2:22 PM Subject: RE: [Histonet] Block Pulling Fees If techs charge for pulling blocks, do ornithologists charge for pulling birds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From otis2k <@t> yahoo.com Thu Jan 22 12:26:32 2004 From: otis2k <@t> yahoo.com (tony filips) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] E1Ajb0N-0004YT-Qs@mk-webmail-2.b2b.uk.tiscali.com Micronuclei Message-ID: <20040122182632.6027.qmail@web20602.mail.yahoo.com> A few years ago when I was doing Gene Tox work we used Acridine orange (pre-coated slides) on blood smears (then coverslipped) for NCE's PCE's and Micronuclei. caveat- the slide folders had to be stored in the freezer (in ziploc bags) as the stain fades with prolonged time at R.T. I'll post the procedure if I can find it. Anthony Filipunas (otis2k@yahoo.com) Histology Manager MPI Research Mattawan, MI __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! http://webhosting.yahoo.com/ps/sb/ From TJJ <@t> Stowers-Institute.org Thu Jan 22 13:37:43 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Re: DAB precipitate Message-ID: Anila Syed wrote: >I have been using DAB successfully for a while, but today when I stained my sections, there were huge clumps of DAB precipitates stuck all over the section. I didn't do anything different and it was a brand new kit. I am sure that I mixed it well as I vortex for about ten seconds prior to use.< You didn't say what vendor you get yours from. We had that problem with Dako's DAB+, we opened a kit that started precipitating on us. We complained to them and they sent a replacement and haven't had that problem with it since. I'd suggest you contact the vendor and let them know what is happening. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From gcallis <@t> montana.edu Thu Jan 22 13:45:25 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] DAB precipitates Help please In-Reply-To: <001401c3e10b$8dcfc6c0$80c401a3@clneuro.ox.ac.uk> References: <20040122064014.13637.qmail@web14903.mail.yahoo.com> <001e01c3e0ff$e6158c40$e3f2acd1@powellsa1> Message-ID: <3.0.6.32.20040122124525.00bd3b28@gemini.msu.montana.edu> If I have a DAB ppt problem, I look for a DAB kit that doesn't produce them!! Some companies have better, less precipitating DAB's than others. It could be a lot problem? Maybe contact company to see if this is a problem. You can microfilter DAB solutions using syringe and microfilter (45 micrometer pore size in nonsterile units (cheaper), then pipette or dispense directly onto. This is messy and another handling of chromogen. At 05:17 PM 1/22/2004 -0000, you wrote: >Dear All, > >I have been using DAB successfully for a while, but today when I stained my >sections, there were huge clumps of DAB precipitates stuck all over the >section. I didn't do anything different and it was a brand new kit. I am >sure that I mixed it well as I vortex for about ten seconds prior to use. > >Is there a way of removing these to see the section underneath? I have tried >copious washing with PBS and left them for a long time in alcohols and >histoclear. >All help gratefully appreciated, > >Anila Syed > > >--- >Outgoing mail is certified Virus Free. >Checked by AVG anti-virus system (http://www.grisoft.com). >Version: 6.0.561 / Virus Database: 353 - Release Date: 13/01/04 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From KHays <@t> mbhs.org Thu Jan 22 15:41:47 2004 From: KHays <@t> mbhs.org (KHays@mbhs.org) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] tbs block printer interface Message-ID: Kathy Tedford-Hays HT (ASCP) Technical Specialist, Histology Dept (601)-968-3070 ext 7398 Baptist Medical Center 1225 North State Street Jackson, MS 39202 i am in need of any information concerning any of the histology labs that are using the scc soft path lab system u that have tbs block printers. we are in the process of trying to gather information for our information systems people so that we can interface the soft path system with a tbs block printer. we are going to upgrade with gui in may. we want to set up a default that automatically prints a certain amount of blocks for each specimen source at the time of accession. someone out in histo land may be doing this already. any ideas would be greatly appreciate. The information contained in this email is confidential and is intended for the recipient only. Do not forward this email without permission from its originator. From SJones <@t> cvm.tamu.edu Thu Jan 22 16:52:32 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] (no subject) Message-ID: The Sevier-Munger or the Grimelius stain can be used for this I believe. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> Anne-Sophie Martinez 1/22/2004 11:02:34 AM >>> Hello everybody, Can I localize the intestinal cells which produce the serotonin, not with an antibody against this hormone, but with a special histological staining? Thank you very much for your help. AS ---------------------------------------------- Dr Anne-Sophie Martinez School of Biology University of St. Andrews Bute Medical Buildings St. Andrews Fife, KY16 9TS UK. Tel + 44 (0) 1334 463546 Fax + 44 (0) 1334 463600 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HACKERLAB <@t> aol.com Thu Jan 22 16:55:41 2004 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Mounting Media for Hacker Coverslippers Message-ID: Dear Histonetters; In response to the exchange between Mary and Jo, please allow me to clarify what appears to be a confusing situation: The coverslipper currently sold by TBS was never a Hacker product nor was it ever sold by Hacker. For a brief period TBS sold the RCM3660 which was previously distributed by Hacker. There is no indication that the mounting media used successfully in our units is compatible with the instrument TBS is now offering. Thanks for your attention, Elfi Hacker HACKER Instruments & Industries Inc. Mary, In reference to the mounting medium for the Hacker- Permount is toluene based. If you use xylene as a clearing agent, you should use a xylene based mounting medium. The toluene based one will dry out real quickly, and you will have coverslips popping off,and lots of extra work. Jo >>> "Mary Parker" 01/22/04 06:53AM >>> ----- Original Message ----- From: Mary Parker To: histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 22, 2004 6:45 AM Subject: Shur/Mount Coverslipper If anyone has a newer model of this automatic coverslipper (formerly Hacker, now TBS), please tell me which mounting medium has worked best. I understand from the sales rep that we can not use Permount on this machine. Any comments? Mary Parker, H.T., A.S.C.P. Histology Manager EPL, Inc. P.O. Box 12766 RTP, N.C. 27709 (919) 998-9407 From HACKERLAB <@t> aol.com Thu Jan 22 16:57:24 2004 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Mounting media for Hacker coverslippers Message-ID: <1a1.1f8b3cd2.2d41af54@aol.com> Dear Histonetters; In response to the exchange between Mary and Jo, please allow me to clarify what appears to be a confusing situation: The coverslipper currently sold by TBS was never a Hacker product nor was it ever sold by Hacker. For a brief period TBS sold the RCM3660 which was previously distributed by Hacker. There is no indication that the mounting media used successfully in our units is compatible with the instrument TBS is now offering. Thanks for your attention, Elfi Hacker HACKER Instruments & Industries Inc. Mary, In reference to the mounting medium for the Hacker- Permount is toluene based. If you use xylene as a clearing agent, you should use a xylene based mounting medium. The toluene based one will dry out real quickly, and you will have coverslips popping off,and lots of extra work. Jo >>> "Mary Parker" 01/22/04 06:53AM >>> ----- Original Message ----- From: Mary Parker To: histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 22, 2004 6:45 AM Subject: Shur/Mount Coverslipper If anyone has a newer model of this automatic coverslipper (formerly Hacker, now TBS), please tell me which mounting medium has worked best. I understand from the sales rep that we can not use Permount on this machine. Any comments? Mary Parker, H.T., A.S.C.P. Histology Manager EPL, Inc. P.O. Box 12766 RTP, N.C. 27709 (919) 998-9407 From Tom_Wells <@t> bcit.ca Thu Jan 22 17:20:34 2004 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Request for digital images Message-ID: I tried sending this email earlier but It didn't appear to go through. If it does make it through I appologize for the duplication. I am teaching a course on Histotechnology and I am looking for digital pictures of various kinds of microtomes (sledge, ultramicrotome, etc.) as well as steel knives and knife sharpening equipment. Since the latter is not routinely used anymore it is difficult to find examples that I can use for my own pictures. Does anyone have any pictures that they wouldn't mind sharing or a website with some images that I could use without breaking any copyrights? I am sure that the students would enjoy seeing what it was like in the "good old days". With thanks. Tom Tom Wells Instructor, Histology BCIT Burnaby, B.C. Canada From DMBCMP <@t> aol.com Thu Jan 22 19:23:07 2004 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Thanks For the Great Response Message-ID: I want to thank all the techs for their responses to my question about embedding W/WO melted paraffin. Just in case anyone is interested, the many replys I got indicate that labs across the US and UK, run about 60/40 in favor of embedding out of melted paraffin. In general, the techs with many years of experience seem to have been trained with this method,.... with the newer techs liking the dry method. The reasons vary, but it really seems to simply be a personal choice. I still prefer the wet method. So good luck to us all and may we never lose someones precious specimen. It COULD be yours!! Thanks again. This was most interesting. Dannie Blake HT(ASCP) Histo Lead Tech Fresno Community Hospital Fresno, California USA From scoop <@t> mail.nih.gov Thu Jan 22 21:29:24 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] long term storage of B-plus fixed bone marrow smears Message-ID: I have some bone marrow smears that I would like to fix in B-plus and then store. I'm thinking that my best option would be to fix the smears, then dehydrate them thru ethanol to xylene and then let them dry for storage. Before staining (I would be doing immunhistochemistry or immunofluorescence) I would rehydrate the smears to water. Has anyone ever stored bone marrow smears fixed in B-plus, B-5 or formalin long term and how did you do it, or does anyone have any advice? Thanks, Sharon -- Sharon Cooperman From ctsblack <@t> capeheart.uct.ac.za Fri Jan 23 00:35:48 2004 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Fixatives for (RDI) Actin on rat tissue. Message-ID: Hi There Does anyone know whether Zinc fixation will adversely affect the staining of Actin in Rat tissue. The method for Actin (RDI anti Human, cross reacts with rat) requires formalin fixation, which we normally do, but as we have done CD 31 on them which required zinc fixing, they are already fixed. The first run has not worked, so if anyone has any experience with this, I would be greatful. Many Thanks Melanie Black. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From r.lederer <@t> uq.edu.au Fri Jan 23 00:57:43 2004 From: r.lederer <@t> uq.edu.au (Rose Lederer) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] aldehyde fuchsin In-Reply-To: Message-ID: <000001c3e17e$32fd5d20$89a6a8c0@vet.uq.edu.au> Dear experts, How good is the (Gomory's) Aldehyde Fuchsin stain to demonstrate the islet cells? Is it used at all? Sorry to bother you but I just wonder if it's worth doing From Stuart.Lamonby <@t> mail.bhrv.nwest.nhs.uk Fri Jan 23 03:51:41 2004 From: Stuart.Lamonby <@t> mail.bhrv.nwest.nhs.uk (Stuart Lamonby) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] RE: Block Pulling Fees Message-ID: <1030B679AD69D6119C3F00080210DD9D01B0B3C4@bhrv-nt-11.bhrv.nwest.nhs.uk> I would be interested to know if any labs, particularly in the UK, charge a fee for pulling either blocks or slides, for example the legal profession when they are requested in pursuit of medico-legal claims. -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: 22 January 2004 15:22 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 2, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to eliminate holes in mouse brain tissue? (Gayle Callis) 2. RE: Another embedding query (Jackie.O'Connor@abbott.com) 3. RE: Another embedding query (Bauer, Karen) 4. Sakura or Leica Cassette Printer (Barbara Stancel) 5. Cole's Hematoxylin (Boneslides@aol.com) 6. Embedding technique (peptolab) 7. Embedding technique (peptolab) 8. Re: CD13 -- FFPE immunohistochemistry (Bryan Hewlett) 9. Re: Cole's Hematoxylin (Bryan Hewlett) 10. HT job opportunity, Sacramento (WWmn916@aol.com) 11. Re: Cole's Hematoxylin (Bryan Llewellyn) 12. Website help (Sonya Hogg) 13. (no subject) (Gagermeier, James) 14. RE: Website help (Nick Kirk) 15. RE: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS Anti-Virus] (G.A.McHardy@arh.grampian.scot.nhs.uk) 16. Rat Micronuclei scoring (ames1@breathe.com) 17. apoptosis/necrosis IHC antibodies (lynsay jackson) 18. Re: Embedding W/WO Melted Paraffin (Gudrun Lang) 19. RE: peltier vs. refrigeration cold stage for slidingmicrotome (Alan Bright) 20. Fw: Shur/Mount Coverslipper (Mary Parker) 21. Re: Fw: Shur/Mount Coverslipper (Joanne Mauger) 22. Re: Block Pulling Fees (Cindy Deriso) 23. RE: Pen (Stacy McLaughlin) 24. pens (BSylinda@aol.com) 25. RE: Block Pulling Fees (Marshall Terry Dr, Consultant Histopathologist) 26. coverslipper justification (DPALLP@aol.com) 27. RE: coverslipper justification (Bartlett, Jeanine) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Jan 2004 15:45:35 -0700 From: Gayle Callis Subject: Re: [Histonet] How to eliminate holes in mouse brain tissue? To: Amy Janes , Histonet@lists.utsouthwestern.edu Message-ID: <3.0.6.32.20040121154535.00bd7798@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii" AMy, Freezing cryoprotected tissue in a cryostat will create freezing artifact (big holes caused by water ice crystal formation with lower temperature/slower freezing in a cryostat). You need to SNAP FREEZE. Histonet archives is filled with suggestions on various ways to snap freeze, do a search as this has been discussed at length recently. Sucrose helps reduce this problem, but it can still rear its ugly head with cryostat freezing. Be sure you cut at a correct temperature also, cryoprotected/prefixed tissue I find are cryosectioning well at around -26C otherwise the sucrose oozes out like Karo syrup. At 02:11 PM 1/21/2004 -0500, you wrote: >Hi, >I am writing to see if I can get some advice on how to eliminate holes I >am seeing in my tissue. >I am using tissue from the mouse olfactory bulb and brain for fos ICC. >Unfortunately I have been unable to see any fos because my tissue is full >of little holes and therefore I have no actual cells. I have determined >that it is not the ICC that is causing the holes because they are present >before staining. So I am assuming I am doing something wrong during >fixation and cryoprotection. I perfuse the animal with 4% para at a rate >of 3.5ml/min and I give the animal around 50mls. I then post fix with 4% >para overnight and then cryoprotect with 30% sucrose until the brain drops >(usually overnight). The brain is then imbedded in OCT and put in the >cryostat at -20C to freeze and cut at 20microns. I typically do not >perfuse with PBS before para but others in my lab have tried perfusing >with and without PBS and have found no difference in tissue quality. Any >suggestions as to how I can fix this? > >Thanks, > Amy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 2 Date: Wed, 21 Jan 2004 16:31:24 -0600 From: Jackie.O'Connor@abbott.com Subject: RE: [Histonet] Another embedding query To: "Stapf, Ross" Cc: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" As long as you have a homogenous block with all your tissue at the appropriate plane - I don't think it much matters. If you thrust cold tissue into molten paraffin, you're going to have tissue popping out because of the lack of homogenous(ness?) Homogeneity? You fill in the noun. If your mold is too cold when you put your tissue in, your block will crumble around the edges. That's why we are artists - each block is unique, and takes a skilled person with some brains (left after formalin fixation) to determine what is best for each block. I've tried everything - that's how I learned what NOT to do. I prefer using room temp molds because some of the newer cassettes have a perfect leak-hole at the bottom where the hinge was - if the mold is as hot as the paraffin, it leaks a lot. If the mold is room temp, it chills faster, and I have less leaking paraffin. Face it - it's a messy job. If we're taking a poll, I keep my cassettes in the warm embedding center sans molten paraffin - but I embed everything in less than an hour. I find for my purposes, the tissue is kept at a decent temp to embed, and the paraffin it was processed in protects it from the air. Seems to work OK for what I'm doing. If it ain't broke, don't fix it. I'm all for heading off disaster, but I don't see any looming on the horizon for us as far as embedding artifacts, etc. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Stapf, Ross" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2004 03:11 PM To: "pam plumlee" , cc: Subject: RE: [Histonet] Another embedding query At my former hospital they embedded dry with room temperature molds. Here they embed dry with hot molds. I think the practice of using room temperature molds at my former job was mostly due to the main embedding tech's preference for ergonomic comfort. They have the old Shandon Embedding Centers and she prefered the molds to be on top of the unit, reach for the tissue with the right hand and the mold with the left. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Wednesday, January 21, 2004 2:45 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Another embedding query Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 21 Jan 2004 17:16:47 -0600 From: "Bauer, Karen" Subject: RE: [Histonet] Another embedding query To: "'Jackie.O'Connor@abbott.com'" , "Stapf, Ross" Cc: histonet@pathology.swmed.edu Message-ID: <8C6E05FA69571948B461F1327CBB893E16C345@LMMAIL2> Content-Type: text/plain; charset="iso-8859-1" I agree with Jackie... We embed almost exactly as Jackie and we've done so for years and years. I've been here for 13 years and we embedded the same at the hospital I worked at before I came here. Never had any problems. Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Wednesday, January 21, 2004 4:31 PM To: Stapf, Ross Cc: histonet@pathology.swmed.edu Subject: RE: [Histonet] Another embedding query As long as you have a homogenous block with all your tissue at the appropriate plane - I don't think it much matters. If you thrust cold tissue into molten paraffin, you're going to have tissue popping out because of the lack of homogenous(ness?) Homogeneity? You fill in the noun. If your mold is too cold when you put your tissue in, your block will crumble around the edges. That's why we are artists - each block is unique, and takes a skilled person with some brains (left after formalin fixation) to determine what is best for each block. I've tried everything - that's how I learned what NOT to do. I prefer using room temp molds because some of the newer cassettes have a perfect leak-hole at the bottom where the hinge was - if the mold is as hot as the paraffin, it leaks a lot. If the mold is room temp, it chills faster, and I have less leaking paraffin. Face it - it's a messy job. If we're taking a poll, I keep my cassettes in the warm embedding center sans molten paraffin - but I embed everything in less than an hour. I find for my purposes, the tissue is kept at a decent temp to embed, and the paraffin it was processed in protects it from the air. Seems to work OK for what I'm doing. If it ain't broke, don't fix it. I'm all for heading off disaster, but I don't see any looming on the horizon for us as far as embedding artifacts, etc. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Stapf, Ross" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/21/2004 03:11 PM To: "pam plumlee" , cc: Subject: RE: [Histonet] Another embedding query At my former hospital they embedded dry with room temperature molds. Here they embed dry with hot molds. I think the practice of using room temperature molds at my former job was mostly due to the main embedding tech's preference for ergonomic comfort. They have the old Shandon Embedding Centers and she prefered the molds to be on top of the unit, reach for the tissue with the right hand and the mold with the left. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Wednesday, January 21, 2004 2:45 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Another embedding query Since the subject of embedding styles is being discussed...I have a new co-worker (great tech with many years experience), that embeds with small amount of paraffin in the holding tank and with room temp. metal molds. I've tried it and don't like it much...maybe I just have to get used to it. So far the only benefit I've seen is a little less paraffin around the blocks-hey, whats a para-trimmer for? Anyone practice or tried this method? Thanks, Pam __________________________________ Do you Yahoo!? Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes http://hotjobs.sweepstakes.yahoo.com/signingbonus _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. ------------------------------ Message: 4 Date: Wed, 21 Jan 2004 23:17:45 +0000 From: "Barbara Stancel" Subject: [Histonet] Sakura or Leica Cassette Printer To: laurie.colbert@huntingtonhospital.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Laurie, We just bought the Sakura cassette and slide autowriters. Love'em. Love'em. Love'em. The only difficult part was training the techs......it took us quite a while to catch on. Me longer than the others. But once we caught on, it has been great. Great service from Sakura also (thanks Sharon Weyman for your hours and hours of patience!). Histologically yours, Barbara USDA, FSIS, OPHS, Eastern Laboratory, Pathology Athens, Georgia 30604 Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Wednesday, January 21, 2004 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Cassette Printer Does anyone out there have or tried out the Leica IP C Cassetter Printer? If so, can you provide any feedback? I am still trying to find a cassette labeler that will work for us. We have tried the Shandon, the TBS, and the new Sakura, but I wasn't totally happy with any of them. Does anyone have info on the annual CSH meeting this year? Laurie Colbert Huntington Hospital Pasadena, CA _________________________________________________________________ There are now three new levels of MSN Hotmail Extra Storage! Learn more. http://join.msn.com/?pgmarket=en-us&page=hotmail/es2&ST=1 ------------------------------ Message: 5 Date: Wed, 21 Jan 2004 18:20:48 EST From: Boneslides@aol.com Subject: [Histonet] Cole's Hematoxylin To: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Does anyone have a procedure for making Cole's Hematoxylin that they would be willing to share?? Thanks in advance! Diane Mahovlic, HT(ASCP) Orthopedic Pathology and Biomaterials Laboratory The Cleveland Clinic Foundation Cleveland, Ohio ------------------------------ Message: 6 Date: Wed, 21 Jan 2004 18:39:58 -0500 From: "peptolab" Subject: [Histonet] Embedding technique To: "HistoNet Server" Message-ID: <000e01c3e078$1b744d50$95a5bd18@JEFF> Content-Type: text/plain; charset="iso-8859-1" I use room temperature molds for everything (Tissue Tek Plastics). The only things that get melted on the warm plate are any needle biopsies- melt the bottom layer, place em in and position, move to cold plate and quickly tamp with the little tamper thingies. The larger sized rectangular molds are banned from my lab. I embed from the holding reservoir filled half way or less. Everything sinks to the bottom and comes out fine, the relative chill though enables most curettings to come out in one cake- easy to transfer to the mold. Tricks: TURP, I gross in a "monolayer" of prostate chips in the cassette. When embedding, I invert the cassette over the base mold and tap, everything falls into place (if your lucky) and there is no need to mess with the chips further. Gunk wrapped in lens tissue can be cooled ever so slightly, scraped off the paper with a slide, as the layer of wax and gunk builds up on the edge, one can warm the bottom of the slide gently and scrape it off in one piece right into the mold. One thing though- I sure miss my bunsen burner. It would keep us warm during these frigid winter days. Jeff Silverman Southside Hospital Bay Shore NY ------------------------------ Message: 7 Date: Wed, 21 Jan 2004 18:43:30 -0500 From: "peptolab" Subject: [Histonet] Embedding technique To: "HistoNet Server" Message-ID: <001301c3e078$6376c010$95a5bd18@JEFF> Content-Type: text/plain; charset="iso-8859-1" I use room temperature molds for everything (Tissue Tek Plastics). They are filled leaving 0.5-1 mm empty at the top. Saves lots of time cleaning the blocks and everything is level and fine at the bottom. The only things that get melted on the warm plate are any needle biopsies- melt the bottom layer, place em in and position, move to cold plate and quickly tamp with the little tamper thingies. The larger sized rectangular molds are banned from my lab. I embed from the holding reservoir filled half way or less. Everything sinks to the bottom and comes out fine, the relative chill though enables most curettings to come out in one cake- easy to transfer to the mold. Tricks: TURP, I gross in a "monolayer" of prostate chips in the cassette. When embedding, I invert the cassette over the base mold and tap, everything falls into place (if your lucky) and there is no need to mess with the chips further. Gunk wrapped in lens tissue can be cooled ever so slightly, scraped off the paper with a slide, as the layer of wax and gunk builds up on the edge, one can warm the bottom of the slide gently and scrape it off in one piece right into the mold. One thing though- I sure miss my bunsen burner. It would keep us warm during these frigid winter days. Jeff Silverman Southside Hospital Bay Shore NY ------------------------------ Message: 8 Date: Wed, 21 Jan 2004 20:51:54 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] CD13 -- FFPE immunohistochemistry To: "HistoNet Server" , "Alex Knisely" Message-ID: <000401c3e08a$50179fa0$6500a8c0@bryanmainbox> Content-Type: text/plain; charset="iso-8859-1" Alex, I haven't tried it, but Novocastra list the following; NCL-CD13-304, clone 38C12, which is reported to work on FFPE following HIER in citrate buffer pH6.0 Worth a try! Regards, Bryan ----- Original Message ----- From: "Alex Knisely" To: "HistoNet Server" Sent: Wednesday, January 21, 2004 6:31 AM Subject: [Histonet] CD13 -- FFPE immunohistochemistry > CD13, also called "aminopeptidase N", is a white-cell antigen usually > assessed by flow cytometry. > > I'd like to look at it in FFPE materials. > > A search through ABCAM has not found a reference to a commercially > available source of an anti-CD13 antibody that works in FFPE sections. > > Can anyone recommend a source and protocol for such an antibody? > > Best thanks in advance > > Alex K > > > Alex Knisely, MD > Consultant Histopathologist > > alex.knisely@kcl.ac.uk > > Institute of Liver Studies > King's College Hospital > Denmark Hill > London SE5 9RS UK > > +44 (0)20 - 7346 - 3125 telefax > +44 (0)20 - 7346 - 4627 office > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 21 Jan 2004 21:03:28 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] Cole's Hematoxylin To: , Message-ID: <001501c3e08b$edb2f470$6500a8c0@bryanmainbox> Content-Type: text/plain; charset="iso-8859-1" Diane, Go to the following for everything you need; http://members.pgonline.com/~bryand/StainsFile/stain/hemalum/cole.htm Regards, Bryan ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 6:20 PM Subject: [Histonet] Cole's Hematoxylin > Does anyone have a procedure for making Cole's Hematoxylin that they would be > willing to share?? > > Thanks in advance! > Diane Mahovlic, HT(ASCP) > Orthopedic Pathology and Biomaterials Laboratory > The Cleveland Clinic Foundation > Cleveland, Ohio > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 21 Jan 2004 21:26:45 EST From: WWmn916@aol.com Subject: [Histonet] HT job opportunity, Sacramento To: histonet@pathology.swmed.edu Message-ID: <192.24f5498e.2d408ee5@aol.com> Content-Type: text/plain; charset="US-ASCII" We are looking for qualified histotechs (ASCP certified, but will consider registry eligible and experienced). We are located in Sacramento, CA. We offer great benefits and hard to beat profit sharing. Yosemite, the ocean, Lake Tahoe, tall redwoods, are geographical attractions in this area. We are a busy laboratory and growing! For more information please call (916) 447-2718. Deborah King, HT (ASCP) Email: WWmn916@aol.com ------------------------------ Message: 11 Date: Wed, 21 Jan 2004 18:39:48 -0800 From: "Bryan Llewellyn" Subject: Re: [Histonet] Cole's Hematoxylin To: "Histonet" , Message-ID: <019601c3e091$036c1300$a370c2cf@bryand> Content-Type: text/plain; charset="iso-8859-1" The formula for Cole's hemalum is on StainsFile; http://stainsfile.info. Choose staining techniques, then mordanted hematoxylin formulae. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 3:20 PM Subject: [Histonet] Cole's Hematoxylin > Does anyone have a procedure for making Cole's Hematoxylin that they would be > willing to share?? > > Thanks in advance! > Diane Mahovlic, HT(ASCP) > Orthopedic Pathology and Biomaterials Laboratory > The Cleveland Clinic Foundation > Cleveland, Ohio > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 12 Date: Thu, 22 Jan 2004 19:40:14 +1300 (NZDT) From: Sonya Hogg Subject: [Histonet] Website help To: histonet@lists.utsouthwestern.edu Message-ID: <20040122064014.13637.qmail@web14903.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am looking for websites relating to histology special staining techniques and am having difficulty does anyone have any good links?? Thanks http://personals.yahoo.com.au - Yahoo! Personals New people, new possibilities. FREE for a limited time. ------------------------------ Message: 13 Date: Thu, 22 Jan 2004 01:56:43 -0500 From: "Gagermeier, James" Subject: [Histonet] (no subject) To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9122C182D4268F45BCB9E64A10B7331F024D1247@1upmc-msx7.isdip.upmc.edu> Content-Type: text/plain Basics of LCM A few questions in regards to laser capture microdissection utilizing a Leica AS LMD System: In regards to buffers to place in the caps - I have routinely used RNA later. This, however, readily crystalizes setting up issues with debris on the microscope. First, is this an acceptable buffer, and second, what are some other buffer alternatives. Furthermore, when preparing slides, I generally store them in a -80 C unit. I generally place them in a zip lock bag - not a heat sealed bag. Also, I do not generally add a dessicant. Should: a) I use heat-sealed bags. If not, do I need to place them in a zip lock at all ?(i.e. can I simply put them in a slide box in the -80C unit) b) Do I need to start using a dessicant? I appreciate any response on these issues. Jim Gagermeier ------------------------------ Message: 14 Date: Thu, 22 Jan 2004 07:33:38 -0000 From: "Nick Kirk" Subject: RE: [Histonet] Website help To: "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Try http://members.pgonline.com/~bryand/StainsFile/ or http://www.nottingham.ac.uk/pathology/default.html or http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sonya Hogg Sent: 22 January 2004 06:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Website help I am looking for websites relating to histology special staining techniques and am having difficulty does anyone have any good links?? Thanks http://personals.yahoo.com.au - Yahoo! Personals New people, new possibilities. FREE for a limited time. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 22 Jan 2004 08:46:46 -0000 From: Subject: [Histonet] RE: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS Anti-Virus] To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone know of a relatively safe, commercially available substitute for acid dichromate glassware cleaning solution? In our neuropathology lab we prepare this solution in-house and it is used for general glassware cleaning and especially for glassware used in silver staining methods. There are some commercial reagents available in the US but I have yet to source one in the UK. Any ideas? Graham A McHardy Pathology Dept ARI Aberdeen > -----Original Message----- > From: histonet-request@lists.utsouthwestern.edu > [SMTP:histonet-request@lists.utsouthwestern.edu] > Sent: 21 January 2004 18:00 > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 2, Issue 29 [Scanned By SOPHOS > Anti-Virus] > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: apotosis marker in mice (Jackie.O'Connor@abbott.com) > 2. Re: Working Saturdays (Gareth Davis) > 3. Hep C (Luis Chiriboga) > 4. RE: HT Certified vs. HT non-certified (Morken, Tim - Labvision) > 5. Recutting Old GMA tissue blocks (Barbara Harris) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Jan 2004 11:25:43 -0600 > From: Jackie.O'Connor@abbott.com > Subject: Re: [Histonet] apotosis marker in mice > To: Susan Q Wells > Cc: histonet-bounces@lists.utsouthwestern.edu, > histonet@pathology.swmed.edu > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > I use Caspase-3 routinely for apoptosis. I use an antibody on zinc fixed > paraffin embedded tissues, but there are some out there for FFPE. > Much easier than TUNEL, and more specific. > > > > > Susan Q Wells > Sent by: histonet-bounces@lists.utsouthwestern.edu > 01/21/2004 10:17 AM > > > To: histonet@pathology.swmed.edu > cc: > Subject: [Histonet] apotosis marker in mice > > > Hello fellow histonetters - Does anyone have a straightforward > protocol for an apotosis marker to use on frozen or paraffin mice > sections.I used the TUNEL stain a number of years ago but I'm > thinking there may be an antibody out there that works just as > well? > Thanks in advance for your time, > Sue Wells HT(ASCP),QIHC > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Wed, 21 Jan 2004 09:28:28 -0800 (PST) > From: Gareth Davis > Subject: Re: [Histonet] Working Saturdays > To: "Weems, Joyce" , Histonet > > Message-ID: <20040121172828.37965.qmail@web13306.mail.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > In our lab at PathGroup, the day shift takes turns to do Saturdays. In > our case we only need one person, because don't do any special stains or > IHC. We basically just cut and get slides ready to be done early Monday > moring. But, the person who is scheduled to work on a Saturday gets the > previous Monday (so that the time works). > Good luck. > > Gareth Blaeuer Davis > Histotechinican > PathGroup > Nashville, Tennessee > > "Weems, Joyce" wrote: > > An unofficial survey: > > How many hospitals are working Saturdays? And what is the case load of > those > that do? > > Thanks for your help! > > Joyce > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. > Thank you. Saint Joseph?EUR(tm)s Health System, Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------- > Do you Yahoo!? > Yahoo! Hotjobs: Enter the "Signing Bonus" Sweepstakes > > ------------------------------ > > Message: 3 > Date: Wed, 21 Jan 2004 12:34:12 -0500 > From: Luis Chiriboga > Subject: [Histonet] Hep C > To: Histonet > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi All > is anyone using/trying/ have an antibody for Hep C > Thanks > Luis > > ------------------------------ > > Message: 4 > Date: Wed, 21 Jan 2004 09:34:45 -0800 > From: "Morken, Tim - Labvision" > Subject: RE: [Histonet] HT Certified vs. HT non-certified > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <0556BE8AC5551E4E8AF6BB9E42509BA2035762@usca0082k08.labvision.apogent.com> > > Content-Type: text/plain > > Yes, the age old question. As usual, it depends...... > > I worked with an uncertified histotech of over 15 years experience who > could > do the usual special stains well, and was an excellet cutter, but who > could > not seem to develop methods on his own, write any meaningful procedures or > even read very well. Basically he was one of those with one year of > experience repeated 15 times. Although he was not certified it was not > for > lack of trying - he tried five times and couldn't pass the written test > (which goes along with the inability to develop procedures, I think, and > he > only attempted certification when others in the lab started a study > group). > But he had some skill so he was an asset to the lab as a general bench > worker. > > I have also worked with formally-trained (ie, completed a course), > certified, very interested, but who were simply mediocre and never > advanced. > > > Then there are those OJT trained techs who got certified by their own hard > work and were absolutely excellent and advanced almost at will (Indeed, I > recently interviewed a non-certified histotech of only two years of > experience (and no formal histotech courses) who had accomplished more in > that two years than most techs do in their entire careers!). > > So, it depends on the person. I tend to be concerned about a person who > has > worked in the field for 15 years and is not certified. That is due either > to > lack of interest, or lack of ability in some way. A person who has > received > the certificaiton has proven their basic skill AND at least some potential > for personal advancement. After all, the test is a personal endeavor; the > person has to do the lab work, the reading, and the test. You don't get > certified just because you occupied a space in the lab for 15 years. And > if > the issue is trainability, probably the certified person is going to be > more > trainiable than someone who can't, or won't attempt to, get certified > > So I guess the short answer is to look into their past very carefully and > see what they have been doing. Never base it simply on years (long or > short) > or certification alone. > > Tim Morken > > > -----Original Message----- > From: Histolady710@aol.com [mailto:Histolady710@aol.com] > Sent: Tuesday, January 20, 2004 4:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HT Certified vs. HT non-certified > > > This is for histology managers ! I'm curious as to which HT you would > hire > - > a non-certified with 15 years experience or a OJT HT (ASCP) with 2 years > experience. Thanks. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Wed, 21 Jan 2004 11:39:48 -0600 > From: "Barbara Harris" > Subject: [Histonet] Recutting Old GMA tissue blocks > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Need helpful tips,protocols,and experienced advise, on how to resoften > or re-embed 12 year old GMA (Glycol Methacrylate)blocked tissues.I will > be sectioning thin section for teaching purposes.Thanks in advance for > any and all responses. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 2, Issue 29 > *************************************** ------------------------------ Message: 16 Date: Thu, 22 Jan 2004 09:20:03 +0000 From: ames1@breathe.com Subject: [Histonet] Rat Micronuclei scoring To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed; charset="utf-8" Hi there, I'm hoping someone can help me with a staining technique to view micronuclei in PCEs and NCEs on rat bone marrow smears. Apparently the best technique is the H&E stain but when I try it the haematoxylin is too strong. I have tried reducing the concentration to 0.5% but the some of the PCEs are still blacked out. Am I doing something wrong? I have resolved the Eosin being overpowering by using aqueous Eosin. I hope everyone is enjoying the New Year. My thanks to anyone who can help, Amy Greenhalgh Scientist Sequani Ltd, Bromyard Road, Ledbury, HR8 1LH ------------------------------ Message: 17 Date: Thu, 22 Jan 2004 10:00:57 +0000 From: "lynsay jackson" Subject: [Histonet] apoptosis/necrosis IHC antibodies To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Hi does anyone know any companies to buy IHC necrosis/apoptosis antibodies from? _________________________________________________________________ Express yourself with cool emoticons - download MSN Messenger today! http://www.msn.co.uk/messenger ------------------------------ Message: 18 Date: Thu, 22 Jan 2004 12:04:18 +0100 From: "Gudrun Lang" Subject: Re: [Histonet] Embedding W/WO Melted Paraffin To: "Histonetliste" , Message-ID: <005d01c3e0d7$7b5bf380$eeeea8c0@SERVER> Content-Type: text/plain; charset="iso-8859-1" In my lab we do the "dry" method for at least twenty years and have no problems. The probes stay in the 60 degree-oven and then in the warm area of the embedding center for about two hours, while embedding. It is cleaner to handle. Lang Gudrun ----- Original Message ----- From: To: Sent: Wednesday, January 21, 2004 4:37 AM Subject: [Histonet] Embedding W/WO Melted Paraffin > Hi, everyone: > In order to settle a difference of opinion between generations of trained > Histotechs in my lab, may I have feedback from anyone interested in responding? > Do you cover the blocks in melted paraffin in the embedding centre reservior > and hold them that way while embedding? or.... > Do you dump them in the warm reservior "dry" (not covered in melted paraffin) > and embed them that way? > Do you consider this "dry" method as bad technique since a tiny biopsy > specimen MAY not be noticed as the paraffin quickly solidifies? > We have a dispute. I have researched every book I can find and there is no > reference to it anywhere. A newly trained tech that came to work for us said > no mention was made during her training period. Some techs did...and some > didn't. As a tech of nearly 27 years, I find this practice to be just asking for > trouble. I was trained to keep everything melted. There seems to be some > argument against keeping the cassettes in the melted paraffin, claiming it "cooks" > the biopsies. I don't buy it, but what are the opinions of others? > It seems so basic to me. I hope this does not come across as frivilous. > Thanks for your input. > > Dannie Blake HT(ASCP) Histology Lead Tech > Fresno Community Medical Centre > Fresno, California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 19 Date: Thu, 22 Jan 2004 11:52:02 -0000 From: "Alan Bright" Subject: RE: [Histonet] peltier vs. refrigeration cold stage for slidingmicrotome To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Kathy, We manufacture both systems the two Peltier solid state type, have cooling outputs for a 30 X 30mm stage is 15watts @ 0?C & 40 X 40mm stage is 25 watts @ 0?C. The refrigerated Stage type cooling output for a 90 x 130mm stage is 300 watts @ 0?C decreasing to 125 watts @ -30?C. With this information you will need to decide which suits the sizes and sectioning temperature of the specimens required for sectioning best, If you require any additional assistance, I will be only to willing to help. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: katherine-walters@uiowa.edu [mailto:katherine-walters@uiowa.edu] Sent: 21 January 2004 14:27 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peltier vs. refrigeration cold stage for slidingmicrotome Dear Histologists, I am beginning the process of looking at cold stages for a sliding microtome. Does anyone have experience or opinions about the usage of peltier stages as opposed to refrigeration stages? I would also appreciate any information on which companies have these for sale. Thank you once again, Kathy Walters _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 22 Jan 2004 06:53:50 -0500 From: "Mary Parker" Subject: [Histonet] Fw: Shur/Mount Coverslipper To: Message-ID: <007c01c3e0de$67955fb0$6b01a8c0@93F1H11> Content-Type: text/plain; charset="Windows-1252" ----- Original Message ----- From: Mary Parker To: histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 22, 2004 6:45 AM Subject: Shur/Mount Coverslipper If anyone has a newer model of this automatic coverslipper (formerly Hacker, now TBS), please tell me which mounting medium has worked best. I understand from the sales rep that we can not use Permount on this machine. Any comments? Mary Parker, H.T., A.S.C.P. Histology Manager EPL, Inc. P.O. Box 12766 RTP, N.C. 27709 (919) 998-9407 ------------------------------ Message: 21 Date: Thu, 22 Jan 2004 07:52:35 -0500 From: "Joanne Mauger" Subject: Re: [Histonet] Fw: Shur/Mount Coverslipper To: mparker@epl-inc.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Mary, In reference to the mounting medium for the Hacker- Permount is toluene based. If you use xylene as a clearing agent, you should use a xylene based mounting medium. The toluene based one will dry out real quickly, and you will have coverslips popping off,and lots of extra work. Jo >>> "Mary Parker" 01/22/04 06:53AM >>> ----- Original Message ----- From: Mary Parker To: histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 22, 2004 6:45 AM Subject: Shur/Mount Coverslipper If anyone has a newer model of this automatic coverslipper (formerly Hacker, now TBS), please tell me which mounting medium has worked best. I understand from the sales rep that we can not use Permount on this machine. Any comments? Mary Parker, H.T., A.S.C.P. Histology Manager EPL, Inc. P.O. Box 12766 RTP, N.C. 27709 (919) 998-9407 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Thu, 22 Jan 2004 08:16:16 -0500 From: "Cindy Deriso" Subject: Re: [Histonet] Block Pulling Fees To: , Message-ID: Content-Type: text/plain; charset=US-ASCII And why not.. I would rather charge at the other end. Filing... at least $5 per slide and 3 per block! >>> "Denise Bland-Piontek" 01/21/04 11:58AM >>> Just wondering how many clinical labs bill for pulling blocks? Our clinical lab just began charging $2.00 per block. Even our pathologists must pay when having blocks pulled for research or education. Just wondering if this is standard? Thanks, Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator WVU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Thu, 22 Jan 2004 08:26:35 -0500 From: Stacy McLaughlin Subject: RE: [Histonet] Pen To: 'Rebecca Barnhart' , histonet@lists.utsouthwestern.edu Message-ID: <3D502BBF5356D31184650090275B750D0346C6C8@mail.cooley-dickinson.org> Content-Type: text/plain We use these pens. I tried to reorder and was told that they were no longer being manufactured. Do you mind sharing your vendor info? Thanks, Stacy -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Wednesday, January 21, 2004 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pen I wanted to share a wonderful discovery with everyone. We have had trouble finding pens that stay on cassette during processing and on slide during staining. We found a pen that works but it dulled rather quickly. We have found a pen that works great. I got it from Ted Pella and it is the RediSharp Plus. Hope this helps anyone that is having the same trouble as us. Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. ------------------------------ Message: 24 Date: Thu, 22 Jan 2004 08:50:25 -0500 From: BSylinda@aol.com Subject: [Histonet] pens To: histonet@lists.utsouthwestern.edu Message-ID: <623063F4.6295A405.00698496@aol.com> Content-Type: text/plain; charset=iso-8859-1 We use a Statmark Pen from Statlab, that is resistant in alcohols and xylene. Statlab Medical Products 18004423573 item # SMP-BK Sylinda ------------------------------ Message: 25 Date: Thu, 22 Jan 2004 13:22:47 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Block Pulling Fees To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" If techs charge for pulling blocks, do ornithologists charge for pulling birds? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk ------------------------------ Message: 26 Date: Thu, 22 Jan 2004 10:08:15 -0500 From: DPALLP@aol.com Subject: [Histonet] coverslipper justification To: histonet@pathology.swmed.edu Message-ID: <34FA2027.1A9497BC.00042A59@aol.com> Content-Type: text/plain; charset=iso-8859-1 Is there a general rule about the volume of slides needed to justify the cost of an automated coverslipper? One of my pathologists seems to think the magical number is 300 slides per day. Thanks. Susie ------------------------------ Message: 27 Date: Thu, 22 Jan 2004 10:17:39 -0500 From: "Bartlett, Jeanine" Subject: RE: [Histonet] coverslipper justification To: , Message-ID: Content-Type: text/plain; charset="us-ascii" I think that whenever you can walk away from a machine while it is performing a task and do other work, then that it is a productive and justified item. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DPALLP@aol.com Sent: Thursday, January 22, 2004 10:08 AM To: histonet@pathology.swmed.edu Subject: [Histonet] coverslipper justification Is there a general rule about the volume of slides needed to justify the cost of an automated coverslipper? One of my pathologists seems to think the magical number is 300 slides per day. Thanks. Susie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 2, Issue 31 *************************************** From RHESSLER <@t> mail.mcg.edu Fri Jan 23 06:12:05 2004 From: RHESSLER <@t> mail.mcg.edu (Richard Hessler) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Rapid Acetylcholinesterase staining. Message-ID: I was wondering if anyone had experience with rapid acetylcholinesterase staining for the interoperative analysis of GI biopsies for Hirshprung's/Neural Intestinal Dysplasia. I found 3 rapid protocols in the litterature, all of which use iso-octamethylpyrophosphoramide (isoOMPA). According to Sigma this chemical is used by the military in nerve gas production and if fatal on contact with skin or inhalation; not something we can use in the frozen section room! Any less toxic alternatives out there? Richard B Hessler, MD Chief, Section of Anatomic Pathology Associate Professor of Pathology and Neurology The Medical College of Georgia -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:internet:rhessler@mail.mcg.edu TEL;WORK:9575 ORG:;Anatomic Pathology EMAIL;WORK;PREF:RHESSLER@mail.mcg.edu N:Hessler;Richard ADR;DOM;WORK;PARCEL;POSTAL:;BAE2571C LABEL;DOM;WORK;PARCEL;POSTAL;ENCODING=QUOTED-PRINTABLE:internet:rhessler@mail.mcg.edu=0A= BAE2571C END:VCARD From mparker <@t> epl-inc.com Fri Jan 23 06:24:55 2004 From: mparker <@t> epl-inc.com (Mary Parker) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Mounting Media for Hacker Coverslippers References: Message-ID: <003001c3e1ab$e9213270$6b01a8c0@93F1H11> For clarification, the coverslipper in question is in fact manufactired and distributed with no ties to Hacker Instruments what so ever. This machine is a Shur/Mark 7000 and is distributed by TBS. My mistake. Any toes stepped upon was purely unintentional. Does anyone else have this unit in their lab? ----- Original Message ----- From: To: Sent: Thursday, January 22, 2004 5:55 PM Subject: [Histonet] Mounting Media for Hacker Coverslippers > Dear Histonetters; > > In response to the exchange between Mary and Jo, please allow me to clarify > what appears to be a confusing situation: > > The coverslipper currently sold by TBS was never a Hacker product nor was it > ever sold by Hacker. For a brief period TBS sold the RCM3660 which was > previously distributed by Hacker. There is no indication that the mounting media used > successfully in our units is compatible with the instrument TBS is now > offering. > > Thanks for your attention, > > Elfi Hacker > HACKER Instruments & Industries Inc. > Mary, > > In reference to the mounting medium for the Hacker- Permount is toluene > based. If you use xylene as a clearing agent, you should use a xylene based > mounting medium. The toluene based one will dry out real quickly, and you will have > coverslips popping off,and lots of extra work. > Jo > > >>> "Mary Parker" 01/22/04 06:53AM >>> > > ----- Original Message ----- > From: Mary Parker > To: histonet-request@lists.utsouthwestern.edu > Sent: Thursday, January 22, 2004 6:45 AM > Subject: Shur/Mount Coverslipper > > > If anyone has a newer model of this automatic coverslipper (formerly Hacker, > now TBS), please tell me which mounting medium has worked best. I understand > from the sales rep that we can not use Permount on this machine. Any comments? > > Mary Parker, H.T., A.S.C.P. > Histology Manager > EPL, Inc. > P.O. Box 12766 > RTP, N.C. 27709 > (919) 998-9407 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mparker <@t> epl-inc.com Fri Jan 23 06:37:09 2004 From: mparker <@t> epl-inc.com (Mary Parker) Date: Fri Sep 16 15:22:29 2005 Subject: Fw: [Histonet] Mounting Media for Hacker Coverslippers Message-ID: <005b01c3e1ad$9e94ce90$6b01a8c0@93F1H11> ----- Original Message ----- From: To: Sent: Thursday, January 22, 2004 5:55 PM Subject: [Histonet] Mounting Media for Hacker Coverslippers > Dear Histonetters; > > In response to the exchange between Mary and Jo, please allow me to clarify > what appears to be a confusing situation: > > The coverslipper currently sold by TBS was never a Hacker product nor was it > ever sold by Hacker. For a brief period TBS sold the RCM3660 which was > previously distributed by Hacker. There is no indication that the mounting media used > successfully in our units is compatible with the instrument TBS is now > offering. > > Thanks for your attention, > > Elfi Hacker > HACKER Instruments & Industries Inc. > Mary, > > In reference to the mounting medium for the Hacker- Permount is toluene > based. If you use xylene as a clearing agent, you should use a xylene based > mounting medium. The toluene based one will dry out real quickly, and you will have > coverslips popping off,and lots of extra work. > Jo > > >>> "Mary Parker" 01/22/04 06:53AM >>> > > ----- Original Message ----- > From: Mary Parker > To: histonet-request@lists.utsouthwestern.edu > Sent: Thursday, January 22, 2004 6:45 AM > Subject: Shur/Mount Coverslipper > > > If anyone has a newer model of this automatic coverslipper (formerly Hacker, > now TBS), please tell me which mounting medium has worked best. I understand > from the sales rep that we can not use Permount on this machine. Any comments? > > Mary Parker, H.T., A.S.C.P. > Histology Manager > EPL, Inc. > P.O. Box 12766 > RTP, N.C. 27709 > (919) 998-9407 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ian.Bernard <@t> LACKLAND.AF.MIL Fri Jan 23 06:47:49 2004 From: Ian.Bernard <@t> LACKLAND.AF.MIL (Bernard Ian R SSgt 59 CRES/MSROP) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Looking for method to make up an Evans blue solution?? Message-ID: <588C513CC306D611A2910003479604F906591C16@fsmpls17.whmc.af.mil> Need a concentration and solvent suggestion to make an Evans blue solution for staining Heart tissue. Traditionally Methylene Blue is used. Should I used alcoholic or Distilled water as solvent?? What good concentration to stain the fresh heart tissue? Thanks B From RBARNHART <@t> summithealth.org Fri Jan 23 08:06:18 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Miracle Pens that stay on anything Message-ID: Several people asked where to order these pens from. Ted Pella (www.tedpella.com) item #27173. Becky From Stanley.Lupo <@t> gsk.com Fri Jan 23 08:40:06 2004 From: Stanley.Lupo <@t> gsk.com (Stanley.Lupo@gsk.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] E1Ajb0N-0004YT-Qs@mk-webmail-2.b2b.uk.tiscali.com Micronuclei Message-ID: Stanley.Lupo@GSK.Com Safety Assessment Mail Code: UE0461 Phone: 610 270-7340 Fax: 610 270-7202 It's been years since I've done the Micronucleus Test in rodents, but we always had good differentiation of polychromatic and normochromatic erythrocytes with the traditional May Gruenwald and Giemsa staining as recommended by the early investigators, Boller and Schmid, Heddle, etc. NCE's were pink to orange, while PCE's were blue. Micronuclei appeared dark purple and were unmistakeable. "tony filips" Sent by: histonet-bounces@lists.utsouthwestern.edu 22-Jan-2004 13:26 To: histonet cc: Subject: [Histonet] E1Ajb0N-0004YT-Qs@mk-webmail-2.b2b.uk.tiscali.com Micronuclei A few years ago when I was doing Gene Tox work we used Acridine orange (pre-coated slides) on blood smears (then coverslipped) for NCE's PCE's and Micronuclei. caveat- the slide folders had to be stored in the freezer (in ziploc bags) as the stain fades with prolonged time at R.T. I'll post the procedure if I can find it. Anthony Filipunas (otis2k@yahoo.com) Histology Manager MPI Research Mattawan, MI __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! http://webhosting.yahoo.com/ps/sb/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chris.goodall <@t> bristol.ac.uk Fri Jan 23 09:10:30 2004 From: chris.goodall <@t> bristol.ac.uk (anclg) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Request for digital images Message-ID: <000401c3e1c3$0a5c7690$8f6ede89@hist143> Hi Tom, I would be very happy to send you some photos of knife sharpening. I still use an old (approx 30 yrs old)Shandon Elliott automatic knife sharpener Mk II for sharpening tunsten carbide knives for resin embedded undecalcified bone. My tissue processor, the Shandon 2L processor Mk II ( the old dip and dunk type)is also still used, as is an old Leitz sledge microtome with peltier freezing stage. I`m in England, lots of antiques here. Chris Goodall From mcauliff <@t> umdnj.edu Fri Jan 23 09:17:22 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] aldehyde fuchsin In-Reply-To: <000001c3e17e$32fd5d20$89a6a8c0@vet.uq.edu.au> References: <000001c3e17e$32fd5d20$89a6a8c0@vet.uq.edu.au> Message-ID: <40113B02.5090101@umdnj.edu> It's beautiful. Fix with Bouin's, wash out the picric acid with several changes of 50% ethanol. The stain is easy to make and apply. Oxidation of the sections is not needed if your basic fuchsin has enough pararosanaline in it. Pararosanaline is the molecule that complexes with the aldehyde to form the stain. In the past, some lots of basic fuchsin did not contain sufficient pararosanaline to give a good reaction so be sure your dye has a high percentage of pararosanaline. Or just order a bottle of Pararosanaline, be sure it is Certified by the Biological Stain Commission. Paraldehyde is a controlled substance and can be difficult to obtain, if you are at a hospital the pharmacy may have it in little glass ampoules which is good because in needs to be fresh. Acetaldehyde, which is easier to get, can be substituted for paraldehyde but you need to use three times as much. See Buehner, Nettleton and Longley, J. Histochem. Cytochem 27:782-787, 1979 for details. Geoff Rose Lederer wrote: >Dear experts, > >How good is the (Gomory's) Aldehyde Fuchsin stain to demonstrate the >islet cells? >Is it used at all? > >Sorry to bother you but I just wonder if it's worth doing > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From kosmicdog <@t> hotmail.com Fri Jan 23 09:35:08 2004 From: kosmicdog <@t> hotmail.com (jason madore) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] making a perfect paraffin block? Message-ID: does anyone have any advice on making a perfect 'blank' paraffin block in the lab (not a pathology lab). This block will be the recipient for a tissue array. How do you avoid internal cracks and how do you get the block out of the mold? also how do you make sure it is strongly attached to the cassette?... any help would be greatly appreciated. thanks J. _________________________________________________________________ Add photos to your messages with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From mcauliff <@t> umdnj.edu Fri Jan 23 09:45:40 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Mounting whole organs in wax In-Reply-To: References: Message-ID: <401141A4.7000104@umdnj.edu> You can buy kits for embedding all sorts of things from Polysciences. I think they are methyacrylate-based. The resulting embeddment is as clear as glass. Wax might look cloudy? Geoff Albert Lee wrote: >Hi, > >Anybody have ideas on how to mount / impregnate whole organs in wax for >display? Has anybody put large tissues on the processor for use in >displays? > >Thanks! Albert > >Albert Lee, BSc., RT (CSLT) >Cardiovascular Registry, the iCAPTURE Centre >St. Paul's Hospital >Room 166, Burrard Building >1081 Burrard Street, Vancouver, BC >Canada V6Z 1Y6 >Phone: 604 - 682 - 2344 extension 63572 >Fax: 604 - 806 - 8158 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Fri Jan 23 10:07:12 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] making a perfect paraffin block? In-Reply-To: Message-ID: <3.0.6.32.20040123090712.00bcd650@gemini.msu.montana.edu> Jason, Buy some disposable plastic molds (Tissue Tek cryomolds) and some tissue cassettes. Melt paraffin at correct melting temperature of paraffin you prefer to use, pour into mold, add cassette and remove mold (disposable are very easy to remove). To harden the paraffin, sit the mold/cassette on a block of ice. Cheap and easy. Fisher has these as does VWR, they are NOT expensive and you can get them in various sizes for your needs. At 07:35 AM 1/23/2004 -0800, you wrote: >does anyone have any advice on making a perfect 'blank' paraffin block in >the lab (not a pathology lab). This block will be the recipient for a tissue >array. How do you avoid internal cracks and how do you get the block out of >the mold? also how do you make sure it is strongly attached to the >cassette?... any help would be greatly appreciated. > >thanks >J. > >_________________________________________________________________ >Add photos to your messages with MSN 8. Get 2 months FREE*. >http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin .msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From juan.gutierrez <@t> christushealth.org Fri Jan 23 10:24:06 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Rapid Acetylcholinesterase staining. Message-ID: The only replacement for isoOMPA that I know of is Ethopropazine and it's no longer available the last I heard. Good luck. Juan -----Original Message----- From: Richard Hessler [mailto:RHESSLER@mail.mcg.edu] Sent: Fri 1/23/2004 6:12 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Rapid Acetylcholinesterase staining. I was wondering if anyone had experience with rapid acetylcholinesterase staining for the interoperative analysis of GI biopsies for Hirshprung's/Neural Intestinal Dysplasia. I found 3 rapid protocols in the litterature, all of which use iso-octamethylpyrophosphoramide (isoOMPA). According to Sigma this chemical is used by the military in nerve gas production and if fatal on contact with skin or inhalation; not something we can use in the frozen section room! Any less toxic alternatives out there? Richard B Hessler, MD Chief, Section of Anatomic Pathology Associate Professor of Pathology and Neurology The Medical College of Georgia From DDittus787 <@t> aol.com Fri Jan 23 12:26:49 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] AP computer Systems Message-ID: <3C6C6048.28438BC9.0A1F969F@aol.com> Once more I am coming to my experts for advice and information. We are currently looking at some AP computer systems for upgrade and I would love to find out what you guys are using, special set ups or needs you found to do with the systems, problems and /or solutions you have encountered. I am not looking at slamming any system, my mind is wide open, just want to hear what everyone is doing and encountering. thanks in advance. Dana Dittus From BrealK <@t> Alexian.net Fri Jan 23 15:07:46 2004 From: BrealK <@t> Alexian.net (Kari Breal) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Phenol congo red Message-ID: Does anyone have a procedure for Phenol Congo Red stain? Kari Breal Histology Supervisor Alexian Brothers Medical Center From cmather <@t> origentherapeutics.com Fri Jan 23 16:21:17 2004 From: cmather <@t> origentherapeutics.com (Christine Mather) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Phosphate buffered saline (PBS) with and without Ca and Mg Message-ID: What are the essential differences in the uses of PBS with and without calcium and magnesium. Why would you use one in favor of the other? Christine. biotech employee From dteleis <@t> vet.upenn.edu Fri Jan 23 16:39:20 2004 From: dteleis <@t> vet.upenn.edu (Donna Teleis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] lifting tissue! Message-ID: <401179B6@webmail.vet.upenn.edu> Okay, so here is my problem... I am cutting Equine Intestine, in MMA, at 3.5 microns, using a Tungsten blade. No problems there. I float them on a drop of 30% ETOH on a slide warmer set at 50 degrees C, and they flatten out nicely. They sit on the warmer overnight. To remove the plastic, I put the slides in warm (40 deg) xylene for 60 minutes, then rinse with 70% ETOH, 50% ETOH, to dist. water. All of this seems to work well, and the tissue sticks on the slide like glue, except for the mucosa. For some reason, when the stained slide dries (H&E stain)the mucosal layer lifts. The rest of the tissue is fine, but that little rim of mucosa just folds right up from the slide. Any thoughts? Ideas? These sections are heading for IHC, and while I know they will survive all the steps while wet, I dont want to loose them when they dry at the end!! Thanks Donna Donna Teleis Research Specialist Department of Clinical Studies Equine Sports Medicine Building University of Pa., New Bolton Center phone: 610-925-6429 fax: 610-925-8131 From BRobert <@t> ameripath.com Fri Jan 23 16:55:54 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] lifting tissue! Message-ID: I had similar problems with thicker stented artery sections and we solved it by leaving slides overnight in xylene at ~60 degrees. You might want to give it a try. Good luck! Brigitte -----Original Message----- From: Donna Teleis [mailto:dteleis@vet.upenn.edu] Sent: Friday, January 23, 2004 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lifting tissue! Okay, so here is my problem... I am cutting Equine Intestine, in MMA, at 3.5 microns, using a Tungsten blade. No problems there. I float them on a drop of 30% ETOH on a slide warmer set at 50 degrees C, and they flatten out nicely. They sit on the warmer overnight. To remove the plastic, I put the slides in warm (40 deg) xylene for 60 minutes, then rinse with 70% ETOH, 50% ETOH, to dist. water. All of this seems to work well, and the tissue sticks on the slide like glue, except for the mucosa. For some reason, when the stained slide dries (H&E stain)the mucosal layer lifts. The rest of the tissue is fine, but that little rim of mucosa just folds right up from the slide. Any thoughts? Ideas? These sections are heading for IHC, and while I know they will survive all the steps while wet, I dont want to loose them when they dry at the end!! Thanks Donna Donna Teleis Research Specialist Department of Clinical Studies Equine Sports Medicine Building University of Pa., New Bolton Center phone: 610-925-6429 fax: 610-925-8131 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joeamateur <@t> hotmail.com Fri Jan 23 16:56:27 2004 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Phosphate buffered saline (PBS) with and without Ca andMg Message-ID: Christine, I'm a newbie to histo, so I can't necessarily speak to the pros and cons for use in staining. My primary training is in cell culture, though, and in those applications, Ca/Mg-free PBS is used to disrupt adherent cells from whatever they're growing on (flasks, slides, etc), prior to applying trypsin (which disrupts them from each other). Hope this helps... --Aloha, Jack England >From: "Christine Mather" >Reply-To: cmather@origentherapeutics.com >To: "Histonet" >Subject: [Histonet] Phosphate buffered saline (PBS) with and without Ca >andMg >Date: Fri, 23 Jan 2004 14:21:17 -0800 > >What are the essential differences in the uses of PBS with and without >calcium and magnesium. Why would you use one in favor of the other? > >Christine. > >biotech employee _________________________________________________________________ Find high-speed ‘net deals — comparison-shop your local providers here. https://broadband.msn.com From cmather <@t> origentherapeutics.com Fri Jan 23 17:08:00 2004 From: cmather <@t> origentherapeutics.com (Christine Mather) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Phosphate buffered saline (PBS) with and without Ca andMg In-Reply-To: <3.0.6.32.20040123154044.00bd1da8@gemini.msu.montana.edu> Message-ID: I am using PBS for making paraformaldehyde fix and also for 30% sucrose for cryoprotecting tissues for both immunostaining and for conventional histochemistry. I have the idea that the calcium and magnesium is required when you are working with tissues as opposed to cells, since the Ca and Mg are important for cell adhesion. With cell culture you want the cells to stay separate so that's when to use the Ca/Mg free PBS. Am I correct? For the sucrose, which is used on fixed tissue, would it still be necessary to use PBS with Ca/Mg, since now the tissues are fixed they should not dissociate if Ca/Mg free PBS? -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, January 23, 2004 2:41 PM To: cmather@origentherapeutics.com Subject: Re: [Histonet] Phosphate buffered saline (PBS) with and without Ca andMg I presume you are using the PBS for immunostaining? You did not say what application was needed. We use Dulbeccos PBS without Ca and Mg for immunostaining of all kinds. For Beta Galactosidase or Beta Gal stained tissue sections/whole lungs, the Dulbeccos has Mg added, probably to aid enzyme reaction and the method has stringent directions. People who do cell cultures and some other molecular biology applications in our department sometimes use Dulbeccos PBS with the Ca and Mg added as essential nutrients? during cell growth. Not being a cell culture type, I may have not answered this completely. I think for most immunostaining purposes, rinses, etc, plain PBS is the standard reagent unless otherwise specified for some special enzyme or other method protocol. If a method calls for Mg and Ca, then you need it. At 02:21 PM 1/23/2004 -0800, you wrote: >What are the essential differences in the uses of PBS with and without >calcium and magnesium. Why would you use one in favor of the other? > >Christine. > >biotech employee > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From MTitford <@t> aol.com Fri Jan 23 18:09:55 2004 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Histology Supervisor Position in Mobile, Alabama. Message-ID: <67791CD9.01C61B86.00762DB1@aol.com> The Clinical Laboratory, University of South Alabama Medical Center in Mobile, Alabama has a vacancy for a Histology Supervisor. The Histology Laboratory is the core laboratory for three hospitals and associated outpatient clinics. The Histology Section includes the Gross room and Histology (H & E's and special stains), immunohistochemistry/ enzyme histochemistry, and Electron Microscopy (Scanning and TEM) with a staff of eight. The laboratory stays busy with clinical, autopsy, research and teaching cases, and histotechnologists work closely with pathologists, residents and medical examiners. If you are a career histotechnologists with a genuine interest in learning more, this may be the position for you! Mobile is located on Mobile Bay near Alabama's Gulf Coast with mild weather (It snowed once a few years ago), fairly low cost of living, Mardi Gras and few traffic jams! If interested, please contact Ms S.Williams at Personnel Relations (251) 471 7325 (FAX) (251) 471 7075 or email at: Swilliams@usouthal.edu 1) Preemployment drug screen required 2) Essential job functions available at Office of Personnel Relations. 3) E.O.E./M.F.D. I would be happy to talk with people offline about this position, but personnel relations handles filling positions, salaries, etc. Thank you! Mike Titford USA Pathology Mobile AL USA From Tom_Wells <@t> bcit.ca Fri Jan 23 18:37:39 2004 From: Tom_Wells <@t> bcit.ca (Tom Wells) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Digital Images Message-ID: I would like to thank everyone who has responded to my request for pictures so far. I really appreciate it. The Histonet is really a remarkable resource. Thank you. Tom From jessgrocki <@t> yahoo.com Fri Jan 23 21:15:41 2004 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Fri Sep 16 15:22:29 2005 Subject: [histonet] unsubscribe Message-ID: <20040124031541.62653.qmail@web41604.mail.yahoo.com> From JCarpenter764 <@t> aol.com Sun Jan 25 08:32:34 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] just a test Message-ID: <77.21156dcf.2d452d82@aol.com> From JCarpenter764 <@t> aol.com Sun Jan 25 09:30:55 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] just a test Message-ID: <77.2116382d.2d453b2f@aol.com> THANKS JIM :-} From dellav <@t> musc.edu Mon Jan 26 07:45:47 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] AP Administrator Vacancy Announcement Message-ID: I am posting this for the University of Arkansas for Medical Sciences in Little Rock VACANCY ANNOUNCEMENT University of Arkansas for Medical Sciences Department of Pathology and Laboratory Services Anatomic Pathology Division Administrator Position Title: Medical Services Manager The Department of Pathology and Laboratory Service at University of Arkansas for Medical Sciences has an immediate opening for a full-time Anatomic Pathology (AP) Division Administrator to oversee the operations of histology and cytology laboratories and transcription unit. The AP Administrator is the business officer for the Division of Anatomic Pathology within the College of Medicine, Department of Pathology. Incumbent reports to the Medical Director of the Anatomic Pathology Division and the Department Administrator. Responsibilities include fiscal management, program and facilities planning, human resources management, and internal and external marketing. The Administrator serves as the administrative liaison with clinical staff and faculty in the College of Medicine, University Hospital, and affiliated hospitals. The Administrator will also act as a partner with the Chairperson in strategic planning and development activities. A Bachelor's degree in Business, Healthcare Administration, or related field plus three years of management/financial experience in a laboratory or related area, preferably in an academic medical center OR a Master's degree in Health Services Administration, Business, or related field plus one year of management/financial experience in a laboratory or related area. Interested individuals should apply at the UAMS Human Resource Office (Recruiting) 4324 W. Markham Street, Little Rock, Arkansas 72205-7199. Questions concerning the position should be addressed to Kelley Suskie, 686-5170 or e-mail ksuskie@uams.edu. The position will close March 31, 2004. From jasmien.taildeman <@t> ugent.be Mon Jan 26 09:04:03 2004 From: jasmien.taildeman <@t> ugent.be (Jasmien Taildeman) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] (no subject) Message-ID: <000e01c3e41d$a2c26820$a55ec19d@PATOL016> Jasmien Taildeman, PhD student Dept. of Pathology (GE22), Block A, fifth floor Faculty of Medicine and Health Sciences Ghent University De Pintelaan 185 B - 9000 Gent Belgium Tel 32-9-2404953 Fax 32-9-2404965 Email: jasmien.taildeman@ugent.be From katherine-walters <@t> uiowa.edu Mon Jan 26 09:05:12 2004 From: katherine-walters <@t> uiowa.edu (katherine-walters@uiowa.edu) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] DRS-601 reservoirs Message-ID: <1075129512.40152ca83cfde@webmail2.its.uiowa.edu> Hi again, I am looking for extra reservoirs to purchase for a Sakura DRS-601 auto- stainer. Please contact me either on line or at (319) 335-8142. Thanks, Kathy From gcallis <@t> montana.edu Mon Jan 26 10:13:00 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Phosphate buffered saline (PBS) with and without CaandMg In-Reply-To: References: <3.0.6.32.20040123154044.00bd1da8@gemini.msu.montana.edu> Message-ID: <3.0.6.32.20040126091300.00bcfdc8@gemini.msu.montana.edu> We add sucrose to Mg and Ca free Dulbeccos PBS for cryoprotection. This is the same DPBS we use for immunostaining but without the sucrose! It works well, and tissues are fine. When immunostaining cells cultured on multiwell chamber slides and destined for immunostaining, we use the same DPBS that we use for frozen sections. At 03:08 PM 1/23/2004 -0800, you wrote: >I am using PBS for making paraformaldehyde fix and also for 30% sucrose for >cryoprotecting tissues for both immunostaining and for conventional >histochemistry. I have the idea that the calcium and magnesium is required >when you are working with tissues as opposed to cells, since the Ca and Mg >are important for cell adhesion. With cell culture you want the cells to >stay separate so that's when to use the Ca/Mg free PBS. Am I correct? > >For the sucrose, which is used on fixed tissue, would it still be necessary >to use PBS with Ca/Mg, since now the tissues are fixed they should not >dissociate if Ca/Mg free PBS? > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: Friday, January 23, 2004 2:41 PM >To: cmather@origentherapeutics.com >Subject: Re: [Histonet] Phosphate buffered saline (PBS) with and without >Ca andMg > > >I presume you are using the PBS for immunostaining? You did not say what >application was needed. We use Dulbeccos PBS without Ca and Mg for >immunostaining of all kinds. For Beta Galactosidase or Beta Gal stained >tissue sections/whole lungs, the Dulbeccos has Mg added, probably to aid >enzyme reaction and the method has stringent directions. > >People who do cell cultures and some other molecular biology applications >in our department sometimes use Dulbeccos PBS with the Ca and Mg added as >essential nutrients? during cell growth. Not being a cell culture type, I >may have not answered this completely. > >I think for most immunostaining purposes, rinses, etc, plain PBS is the >standard reagent unless otherwise specified for some special enzyme or >other method protocol. If a method calls for Mg and Ca, then you need it. > >At 02:21 PM 1/23/2004 -0800, you wrote: >>What are the essential differences in the uses of PBS with and without >>calcium and magnesium. Why would you use one in favor of the other? >> >>Christine. >> >>biotech employee >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From lizchlipala <@t> premierhistology.com Mon Jan 26 13:17:36 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] cryostat sections of undecalcified tissue implants Message-ID: <000001c3e441$11f54640$74d48a80@LIZ> Hello all Is it possible to section undecalcified cryostat sections from implants (no plastic) from a rat subcutaneous implant animal model. The implants will have possibly new bone and cartilage, not very dense bone, but some bone. Would I need to use a tape transfer system or could I use a tungsten knife and be o.k. Any suggestions would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From pruegg <@t> colobio.com Mon Jan 26 13:45:33 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] cryostat sections of undecalcified tissue implants In-Reply-To: <000001c3e441$11f54640$74d48a80@LIZ> Message-ID: Liz, It depends on how big and crunchy it is. Dabbs method for cutting fs of bone marrow biopsies undecalcified worked pretty well on small samples. He embedded in (I can't think of the name of it off the top of my head) but it set up harder than OCT. I have done whole rat femurs without decal using the tape transfer system and D profile tungsten carbide knives. I have also done fs on osicles of collagen sponges treated with growth factors to stimulate bone and cartilage formation. Some of them at early stages could be cut in the cryostat without using the tape and the more developed one's could not. Hopes this helps. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Elizabeth Chlipala Sent: Monday, January 26, 2004 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat sections of undecalcified tissue implants Hello all Is it possible to section undecalcified cryostat sections from implants (no plastic) from a rat subcutaneous implant animal model. The implants will have possibly new bone and cartilage, not very dense bone, but some bone. Would I need to use a tape transfer system or could I use a tungsten knife and be o.k. Any suggestions would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Myri37 <@t> aol.com Mon Jan 26 14:08:06 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] SBF HELP !! Message-ID: Please does anyone have a true protocol to preparate SBF (simulated body fluid) i tried to prepare this solution about 15 times, and whitout any result.. Myriam From froyer <@t> bitstream.net Mon Jan 26 13:56:34 2004 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Cassmark II Cassette Laberler Message-ID: <401570F2.6060906@bitstream.net> I am posting this for a researcher that does not have access to the web. She has inherited a Surgipath Cassmark II cassette labeler from a local university, but the operator manual is missing. If anyone out there in Histoland has one, could you please contact me off-list. Thanks, ~ Ford Ford Royer Analytical Instruments, llc Minneapolis, MN From gcallis <@t> montana.edu Mon Jan 26 14:41:30 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] cryostat sections of undecalcified tissue implants In-Reply-To: <000001c3e441$11f54640$74d48a80@LIZ> Message-ID: <3.0.6.32.20040126134130.00be2a70@gemini.msu.montana.edu> Tape transfer but try TC knife first, bone will give you the most problem but you may survive with a really sharp, new edge. You will have to set the temperature cold, -28C to -30C usually works. At 12:17 PM 1/26/2004 -0700, you wrote: > >Hello all > >Is it possible to section undecalcified cryostat sections from implants >(no plastic) from a rat subcutaneous implant animal model. The implants >will have possibly new bone and cartilage, not very dense bone, but some >bone. Would I need to use a tape transfer system or could I use a >tungsten knife and be o.k. Any suggestions would be appreciated. > >Thanks in advance > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP) >Premier Histology Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >lizchlipala@premierhistology.com >www.premierhistology.com > >Ship to Address: >Premier Histology Laboratory >University of Colorado >MCBD, Room A3B40 >Boulder, Colorado 80309 > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachments, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, >printing, or copying of this email or any attached files is strictly >prohibited. If you have received this email in error, please immediately >purge it and all attachments and notify the sender by reply email or >contact the sender at the number listed above if one is provided. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon Jan 26 14:50:26 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] cryostat sections of undecalcified tissue implants In-Reply-To: References: <000001c3e441$11f54640$74d48a80@LIZ> Message-ID: <3.0.6.32.20040126135026.00be2a70@gemini.msu.montana.edu> Patsy et al, RA Dodds did not embed his bone samples, he coated them with 4% polyvinyl alcohol (70,000 MW water soluble) then dropped them into a dry ice/Hexane mixture to snap freeze. After freezing, he placed bone on dry ice to evaporate the nasty hexane fumes, and mounted the bones on a metal chuck with the 4% PVA. Personally, I do not like this mixture as an embedding media, OCT works just as well. OCT does have PVA in it, but which one is proprietary, MW unknown, this is combined with Polyethylene glycol (MW unknown) and is softer than Dodds method, but with tape transfer, we have no problems with OCT. You can use OCT, and we dilute it 1:1 with water to do the coating for that method. It works just as well as the 4% PVA. Most of the time, we embed bone directly in OCT, which helps surround the sample for support during sectioning. Dodds has a publication in J O Histotechnology some years back, and John Tarpley has a wonderful publication last year on sectioning bone with the Cryojane tape transfer. The methods are worth reading. At 12:45 PM 1/26/2004 -0700, you wrote: >Liz, >It depends on how big and crunchy it is. Dabbs method for cutting fs of >bone marrow biopsies undecalcified worked pretty well on small samples. He >embedded in (I can't think of the name of it off the top of my head) but it >set up harder than OCT. >I have done whole rat femurs without decal using the tape transfer system >and D profile tungsten carbide knives. I have also done fs on osicles of >collagen sponges treated with growth factors to stimulate bone and cartilage >formation. Some of them at early stages could be cut in the cryostat >without using the tape and the more developed one's could not. >Hopes this helps. >Patsy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Elizabeth >Chlipala >Sent: Monday, January 26, 2004 12:18 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] cryostat sections of undecalcified tissue implants > > > >Hello all > >Is it possible to section undecalcified cryostat sections from implants >(no plastic) from a rat subcutaneous implant animal model. The implants >will have possibly new bone and cartilage, not very dense bone, but some >bone. Would I need to use a tape transfer system or could I use a >tungsten knife and be o.k. Any suggestions would be appreciated. > >Thanks in advance > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP) >Premier Histology Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >lizchlipala@premierhistology.com >www.premierhistology.com > >Ship to Address: >Premier Histology Laboratory >University of Colorado >MCBD, Room A3B40 >Boulder, Colorado 80309 > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachments, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, >printing, or copying of this email or any attached files is strictly >prohibited. If you have received this email in error, please immediately >purge it and all attachments and notify the sender by reply email or >contact the sender at the number listed above if one is provided. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gregj <@t> paradise.net.nz Mon Jan 26 15:42:40 2004 From: gregj <@t> paradise.net.nz (Greg Jacobson) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Nuclear-specific counterstain for wholemount mouse mammary gland Message-ID: <000501c3e455$55f5c8b0$3e1ef6d2@workhorse> Hi Histonetters, I need a nuclear-specific counterstain for ?-gal (mosaically) stained wholemount mouse mammary gland. I would prefer not having to section this tissue as I am observing gland wide ?-gal staining directly on the fat-cleared wholemount. To date I have been staining with carmine alum. Thanks for your helpful suggestions on this one, Greg Jacobson The University of Waikato Hamilton New Zealand gregj@paradise.net.nz From sonyalhogg <@t> yahoo.co.nz Mon Jan 26 20:14:18 2004 From: sonyalhogg <@t> yahoo.co.nz (=?iso-8859-1?q?Sonya=20Hogg?=) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] ? Masson Trichrome Message-ID: <20040127021418.39438.qmail@web14903.mail.yahoo.com> In a masson trichrome would you replace PMA with HCL? http://personals.yahoo.com.au - Yahoo! Personals New people, new possibilities. FREE for a limited time. From CCLYATT <@t> mail.mcg.edu Tue Jan 27 05:02:49 2004 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Looking for Freida Carson Message-ID: Freida, I need to discuss something with you. Please give me a number I can call. Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 e-mail: cclyatt@mail.mcg.edu From asmith <@t> mail.barry.edu Tue Jan 27 08:09:41 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Limax flavus lectin Message-ID: <494304423C63E246A5CF87A3AEEB577011EDEA@bumail01.barrynet.barry.edu> Does anyone know of a supplier who has Limax flavus lectin (preferably conjugated) in stock? Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From abright <@t> brightinstruments.com Tue Jan 27 09:33:02 2004 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] cryostat sections of undecalcified tissue implants Message-ID: Dear Elizabeth, Our cryostats fitted with the 5030 & 5040 have no problem sectioning the bone you mention, using a standard C profile Tungsten carbide tipped knife and temperature settings for the cryochamber @ -28 to -30?C. By using a C profile knife the bone is delivered onto the knife face through the standard anti-roll system without the stress effect caused by D profile knives, which also disposes of the need for the costly and slow, tape transfer method. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Elizabeth Chlipala [mailto:lizchlipala@premierhistology.com] Sent: 26 January 2004 19:18 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat sections of undecalcified tissue implants Hello all Is it possible to section undecalcified cryostat sections from implants (no plastic) from a rat subcutaneous implant animal model. The implants will have possibly new bone and cartilage, not very dense bone, but some bone. Would I need to use a tape transfer system or could I use a tungsten knife and be o.k. Any suggestions would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jan 27 10:11:02 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Limax flavus lectin In-Reply-To: <494304423C63E246A5CF87A3AEEB577011EDEA@bumail01.barrynet.b arry.edu> Message-ID: <3.0.6.32.20040127091102.00bdb108@gemini.msu.montana.edu> Did you try Vector Laboratories, at least their technical services. Ask this fellow who is their tech rep, cspow@vectorlabs.com . Craig is extremely helpful and if anything, he could direct you to a source. Vector has excellent lectin information. At 09:09 AM 1/27/2004 -0500, you wrote: >Does anyone know of a supplier who has Limax flavus lectin (preferably >conjugated) in stock? > >Allen A. Smith, Ph.D. >Professor of Anatomy >School of Graduate Medical Sciences >Barry University >Miami Shores, FL > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From pruegg <@t> colobio.com Tue Jan 27 11:18:03 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] cryostat sections of undecalcified tissue implants In-Reply-To: <3.0.6.32.20040126135026.00be2a70@gemini.msu.montana.edu> Message-ID: Gayle, what I meant was that Dabbs used the pva instead of OCT. I tried his method back in the day and I did like that the pva set up harder it seemed to be more of the consistantancie of the bone I was trying to cut (small bone/marrow biopsies) I found Dabbs method to be limited to very small samples without a lot of calcification. This may be fine for Liz as I do not think that at two weeks her explants will be very calcified. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Monday, January 26, 2004 1:50 PM To: Patsy Ruegg; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat sections of undecalcified tissue implants Patsy et al, RA Dodds did not embed his bone samples, he coated them with 4% polyvinyl alcohol (70,000 MW water soluble) then dropped them into a dry ice/Hexane mixture to snap freeze. After freezing, he placed bone on dry ice to evaporate the nasty hexane fumes, and mounted the bones on a metal chuck with the 4% PVA. Personally, I do not like this mixture as an embedding media, OCT works just as well. OCT does have PVA in it, but which one is proprietary, MW unknown, this is combined with Polyethylene glycol (MW unknown) and is softer than Dodds method, but with tape transfer, we have no problems with OCT. You can use OCT, and we dilute it 1:1 with water to do the coating for that method. It works just as well as the 4% PVA. Most of the time, we embed bone directly in OCT, which helps surround the sample for support during sectioning. Dodds has a publication in J O Histotechnology some years back, and John Tarpley has a wonderful publication last year on sectioning bone with the Cryojane tape transfer. The methods are worth reading. At 12:45 PM 1/26/2004 -0700, you wrote: >Liz, >It depends on how big and crunchy it is. Dabbs method for cutting fs of >bone marrow biopsies undecalcified worked pretty well on small samples. He >embedded in (I can't think of the name of it off the top of my head) but it >set up harder than OCT. >I have done whole rat femurs without decal using the tape transfer system >and D profile tungsten carbide knives. I have also done fs on osicles of >collagen sponges treated with growth factors to stimulate bone and cartilage >formation. Some of them at early stages could be cut in the cryostat >without using the tape and the more developed one's could not. >Hopes this helps. >Patsy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Elizabeth >Chlipala >Sent: Monday, January 26, 2004 12:18 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] cryostat sections of undecalcified tissue implants > > > >Hello all > >Is it possible to section undecalcified cryostat sections from implants >(no plastic) from a rat subcutaneous implant animal model. The implants >will have possibly new bone and cartilage, not very dense bone, but some >bone. Would I need to use a tape transfer system or could I use a >tungsten knife and be o.k. Any suggestions would be appreciated. > >Thanks in advance > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP) >Premier Histology Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >lizchlipala@premierhistology.com >www.premierhistology.com > >Ship to Address: >Premier Histology Laboratory >University of Colorado >MCBD, Room A3B40 >Boulder, Colorado 80309 > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachments, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, >printing, or copying of this email or any attached files is strictly >prohibited. If you have received this email in error, please immediately >purge it and all attachments and notify the sender by reply email or >contact the sender at the number listed above if one is provided. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann <@t> lan1.molonc.mcgill.ca Tue Jan 27 12:46:08 2004 From: jo-ann <@t> lan1.molonc.mcgill.ca (jo-ann) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Kryofix Message-ID: Hi to all Can someone tell me if you have heard of a product called "Kryofix"? Where can I get it in Canada? Thanks Jo-Ann Bader From kspencer <@t> utmem.edu Tue Jan 27 13:01:58 2004 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Kryofix In-Reply-To: Message-ID: <4849A1A7-50FB-11D8-BD58-000393967904@utmem.edu> EM Science's has it. As far as Canada goes, I have no idea. Just do an on-line search for kryofix. -Kathleen On Tuesday, January 27, 2004, at 12:46 PM, jo-ann wrote: > Hi to all > > Can someone tell me if you have heard of a product called "Kryofix"? > Where > can I get it in Canada? > > Thanks > > Jo-Ann Bader > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeannette.Mitchell <@t> vtmednet.org Tue Jan 27 13:05:03 2004 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] FW: ASRs Message-ID: > Can folks tell me what they are doing with regard to CAP Checklist Dec. 2003 > question # ANP.12425 regarding ASRs (Analyte Specific Reagents) ? > We currently do not have any disclaimer in our patient surgical reports because > all our antibodies are commercially produced (not "home brews"). > In reading this recently revised requirement however it sounds like we need to have this > statement in all our reports that have had IHC performed. > Most of the antibodies we use have package inserts saying for "Research Use Only". > > I'd appreciate any feedback as to whether the CAP recommended disclaimer is necessary > with commercially bought antibodies (not in kits). > thanks > Jude > Jude Carpenter, BS, HTL(ASCP) > Chief Technologist for Autopsy/Histology/Surgical Pathology > 111 Colchester Ave. > Burlington, VT 05401 > jude.carpenter@vtmednet.org > (802)847-5116 > fax: (802)847-3509 > Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From HornHV <@t> archildrens.org Tue Jan 27 13:42:39 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] FW: ASRs Message-ID: The disclaimer is necessary if the antibody is labeled ASR by the manufacturer. If it is, you must use the disclaimer. Almost all of our antibodies are IVD or ASR and we bill for those. But, if we have a RUO antibody (we have 2) we do not bill for those immunos. It's not a CAP requirement, it's a CLIA requirement for the statement and CAP is just following through with their inspections to see if labs are complying. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org] Sent: Tuesday, January 27, 2004 1:05 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] FW: ASRs > Can folks tell me what they are doing with regard to CAP Checklist > Dec. 2003 question # ANP.12425 regarding ASRs (Analyte Specific > Reagents) ? We currently do not have any disclaimer in our patient > surgical reports because all our antibodies are commercially produced > (not "home brews"). In reading this recently revised requirement > however it sounds like we need to have this statement in all our > reports that have had IHC performed. Most of the antibodies we use > have package inserts saying for "Research Use Only". > > I'd appreciate any feedback as to whether the CAP recommended > disclaimer is necessary with commercially bought antibodies (not in > kits). thanks Jude > Jude Carpenter, BS, HTL(ASCP) > Chief Technologist for Autopsy/Histology/Surgical Pathology > 111 Colchester Ave. > Burlington, VT 05401 > jude.carpenter@vtmednet.org > (802)847-5116 > fax: (802)847-3509 > Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Myri37 <@t> aol.com Tue Jan 27 14:22:45 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] von kossa stain Message-ID: <1c0.145a8f54.2d482295@aol.com> hello everyone Do you know if does von kossa stain give a positif result with any calcium salts ? i think the most common ions are phosphate and carbonates, but if it is "calcium titanate" do you think if this salt is soluble and can give a positive result with von kossa stain ? thank you for any help Myriam From DDittus787 <@t> aol.com Tue Jan 27 14:23:18 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Message-ID: <64C52E2D.14B48113.0A1F969F@aol.com> Hi, once more i am asking for your assistance, what are you guys doing, when two vas or fallopian tube come in one container? as i understand it, for compliance when one container one cassette, you cannot designate right and left unless indicated.am i barking up the wrong tree or is this the compliance issue so that all things are correct. help me out here. thanks Dana From pruegg <@t> colobio.com Tue Jan 27 14:47:15 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Pecam-1 (CD31) In-Reply-To: Message-ID: does anyone know of a rabbit cd31 or of an anti-goat labeled polymer detection reagent. my mouse livers have too much endogenous biotin even with very aggressive a/b block to use an avidin/biotin detection system. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela McNabola Sent: Tuesday, January 20, 2004 10:08 AM To: Loralee_Gehan@URMC.Rochester.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] Pecam-1 (CD31) Hi, We use Santa Cruz PECAM-1 M-20 (SC-15060) goat polyclonal. We steam for 30 minutes in Dako TRS (S1699) for 30 minutes (cool to room temperature). We also avidin/biotin block (Vector), and use ABC (Vector elite kit). Works great, especially on mouse xenografts. Let me know if you need more information. Angela McNabola, MS, HT(ASCP)SLS Bayer Corp. West Haven, CT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCarpenter764 <@t> aol.com Tue Jan 27 15:20:16 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Message-ID: <7e.45aacb1b.2d483010@aol.com> In our histo lab when we receive one container for vas or fallopian tubes the surgical requistion usually indicates that one ( the right or left is tagged) in that case we make out two blocks for that one container and the pathologist dictates which one is tagged or marked. Hope this helps Jennell From SCheasty <@t> ahs.llumc.edu Tue Jan 27 15:35:07 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Message-ID: <2E50F33F91EEDA46A77BC3B2575BB09105874B@mars.llumc.edu> If the submitting physician does not choose to differentiate left from right, it's out of pathology's hands. If the pathologist feels that this is not acceptable, (such as a left and right breast biopsy) she can address it directly with the submitting doc, through the institution's QI committee, change the lab's acceptance policy etc. With vas, most places would let the submitting doc make that choice to designate it or not. When I gross 2 vas received in the same container, unless they are identical, I always ink one of them and dictate which one was inked. ("The longer-larger diameter-curved-etc specimen is inked black, serially sectioned, yadda-yadda-yadda") If I was a doc doing vasectomies or tubals, I would want to know which side was which, just in case the path report came back stating that one of them was not vas or f. tube. But then again, physicians do many things that puzzle me. Sandy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DDittus787@aol.com Sent: Tuesday, January 27, 2004 12:23 To: Histonet@pathology.swmed.edu Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Hi, once more i am asking for your assistance, what are you guys doing, when two vas or fallopian tube come in one container? as i understand it, for compliance when one container one cassette, you cannot designate right and left unless indicated.am i barking up the wrong tree or is this the compliance issue so that all things are correct. help me out here. thanks Dana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From pruegg <@t> colobio.com Tue Jan 27 15:32:09 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER In-Reply-To: <64C52E2D.14B48113.0A1F969F@aol.com> Message-ID: Dana I can't answer your question but this reminds me of something that really happened to me. Bare with me folks I am in a mood..... There was once a cytotechnologists who was not allowed to screen cytologies anymore because they had signed out too many positive cases as negative. The department could not get rid of this person outright (don't ask me why not) so they made them work as a gofer for all the other labs. I was running the heme/path lab at the time by myself and very understaffed. Besides all the laboratory duties I had I also assisted with bone marrow biopsies which took me out of the lab away from my duties even more. Since the demoted cytotech had experience with assisting with FNA's etc., the administration put them on bone marrow assistance duty to help me out. When word got around that this person was helping out, my pathologists came to me an said specifically "do not let that person touch my samples". Well, the admin. person said send them on bm duty anyway. On a very busy day, this person came back to my lab with one vial with 2 bone marrow biopsies in the vial and said to me as they handed me the vial "the longer sample belongs to patient Joe Blow and the shorter sample belongs to patient Mary Doe". The tech had run out of fixative vials and decided to put two samples from two different patients in the same vial instead of coming back to the lab for more vials. Imagine my horror! I carefully recorded the description of which sample belonged to who so that at least that would not get lost. I then walked the tech with the vial with 2 bm samples in it to the office of the administrator and said that I would not take responsible for this situation and wanted nothing to do with it. I also told the admin. what my pathologists had just said to me about not letting that person touch their samples. I was told to process the samples as usual and label them as had been described. I did. As it turned out there was no difference in diagnosis in these two samples. They were the same. These were renal patients being biopsied to test for aluminum bone disease and they both were negative. Imagine if it had come out some other way. One positive and one neg. or if they were leukemia patients and one was in remission and the other not. As I recall it still took months to finally get rid of this person, but they did not do bm assistant duty anymore. It matters what we do and how we do it! Best regards, Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of DDittus787@aol.com Sent: Tuesday, January 27, 2004 1:23 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Hi, once more i am asking for your assistance, what are you guys doing, when two vas or fallopian tube come in one container? as i understand it, for compliance when one container one cassette, you cannot designate right and left unless indicated.am i barking up the wrong tree or is this the compliance issue so that all things are correct. help me out here. thanks Dana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wayneholland1959 <@t> msn.com Tue Jan 27 17:17:25 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Message-ID: I have a new twist to this question of two or more specimens in one container. Often we receive two or more skin lesions, but mostly skin punch biopsies in one specimen container from outside doctors offices. However; usually these specimens are labeled as the same area of excision, but obvious distinct different sites. I have expressed my concern for obvious reasons, both for the pathologist and the physician. But the main question I need answers to is, can we separate these specimens into a,b,c- etc; ??? I guess I have not looked in the right place for the answer, until now hopefully. Is it legal to separate and charge accordingly? I know we can separate and process in a number of ways, but this will not provide correct billing. Any feedback would be great. >From: "Patsy Ruegg" >To: , >Subject: RE: [Histonet] TWO SPECIMENS IN ONE CONTAINER >Date: Tue, 27 Jan 2004 14:32:09 -0700 > >Dana I can't answer your question but this reminds me of something that >really happened to me. Bare with me folks I am in a mood..... > >There was once a cytotechnologists who was not allowed to screen cytologies >anymore because they had signed out too many positive cases as negative. >The department could not get rid of this person outright (don't ask me why >not) so they made them work as a gofer for all the other labs. I was >running the heme/path lab at the time by myself and very understaffed. >Besides all the laboratory duties I had I also assisted with bone marrow >biopsies which took me out of the lab away from my duties even more. Since >the demoted cytotech had experience with assisting with FNA's etc., the >administration put them on bone marrow assistance duty to help me out. >When >word got around that this person was helping out, my pathologists came to >me >an said specifically "do not let that person touch my samples". Well, the >admin. person said send them on bm duty anyway. On a very busy day, this >person came back to my lab with one vial with 2 bone marrow biopsies in the >vial and said to me as they handed me the vial "the longer sample belongs >to >patient Joe Blow and the shorter sample belongs to patient Mary Doe". The >tech had run out of fixative vials and decided to put two samples from two >different patients in the same vial instead of coming back to the lab for >more vials. Imagine my horror! I carefully recorded the description of >which sample belonged to who so that at least that would not get lost. I >then walked the tech with the vial with 2 bm samples in it to the office of >the administrator and said that I would not take responsible for this >situation and wanted nothing to do with it. I also told the admin. what my >pathologists had just said to me about not letting that person touch their >samples. >I was told to process the samples as usual and label them as had been >described. I did. As it turned out there was no difference in diagnosis >in these two samples. They were the same. These were renal patients being >biopsied to test for aluminum bone disease and they both were negative. >Imagine if it had come out some other way. One positive and one neg. or if >they were leukemia patients and one was in remission and the other not. As >I recall it still took months to finally get rid of this person, but they >did not do bm assistant duty anymore. It matters what we do and how we do >it! >Best regards, >Patsy > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >DDittus787@aol.com >Sent: Tuesday, January 27, 2004 1:23 PM >To: Histonet@pathology.swmed.edu >Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER > > >Hi, once more i am asking for your assistance, what are you guys doing, >when >two vas or fallopian tube come in one container? as i understand it, for >compliance when one container one cassette, you cannot designate right and >left unless indicated.am i barking up the wrong tree or is this the >compliance issue so that all things are correct. help me out here. >thanks > Dana > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 From AnthonyH <@t> chw.edu.au Tue Jan 27 17:38:37 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Phosphate buffered saline (PBS) with and without C a and Mg Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E11C@simba.kids> Christine, Ca and Mg ions are often included in lectin diluents when different sugars are being demonstrated. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Christine Mather [mailto:cmather@origentherapeutics.com] Sent: Saturday, 24 January 2004 9:21 AM To: Histonet Subject: [Histonet] Phosphate buffered saline (PBS) with and without Ca and Mg What are the essential differences in the uses of PBS with and without calcium and magnesium. Why would you use one in favor of the other? Christine. biotech employee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From pam <@t> ategra.com Tue Jan 27 15:49:57 2004 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:22:29 2005 Subject: [Histonet] Job Opportunities in Histology Latest Update 01/27/04 Message-ID: Hi Histotech, Here is the latest update on opportunities with some of my best clients throughout the US who are seeking Histology Supervisors, Histo Technologists and Histo Technicians. These are positions as direct employees of our client. There are fulltime 40 hour per week positions.. As a direct employee of one of our clients you will be provided with full benefits including Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative sign-on bonus. I have part time and temporary positions as well. I have supervisory, team lead and bench positions. These positions require HTL or HT certification or registry eligibility. Here are some of my HOTTEST Histology Supevisory positions: 1. Maine - Histology Supervisor 2. Upstate NY - Histology Supervisor 3. Southern IN - Histology Supervisor 4. Philadelphia, PA - Histology Supervisor 5. Kansas City, MO - Histology Supervisor 6. Northern, VA - Histology Supervisor 7. Denver, CO - Histology Supervisor Here are some of my HOTTEST Histo Tech bench positions: 1. Southern Florida - Histo Tech 3. Central VA - Histo Tech 4. Pittsburgh, PA - Histo Tech 5. Oregon - Histo Tech 6. San Antonio area, TX - Histo Tech 7. Boston, MA - Histo Tech (part time 2 positions) 8. Boston, MA - MOHS Tech 9. Upstate NY - Histo Tech 10. Kansas City KS - Lead Histo Tech 11. Southern IN - Lead Histo Tech 12. Northern VA - Lead Histo Tech If you are interested in these jobs, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well. I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who do not have an email address, if you could pass my query & name on to them I'd be very grateful. I don't want to be a bother - I was told that you were a hands-on Histo Tech or a Lab Supervisor. If you are no longer working in a lab please send me an email and I will remove you from my list of people to contact. However, if you are interested in any of the jobs above, please call me. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: Pam Barker Senior Lab Recruiter Ategra Systems Inc Specialists in Permanent & Contract Staffing 7085 University Blvd. Winter Park, FL 32792 VOICE: 407-671-5800 ext 234 TOLLFREE: 800-466-9919 ext 234 EMAIL: pam@ategra.com WEBSITE: If you received this email in error, or if you wish to receive no more emails from us, please send me an email to the above address and you will be deleted from our prospect list. For faster results, please tell us the email address we used to email you, (in your case it is "Histonet@pathology.swmed.edu". ---------------------------------------------------------------- --------------------------------------------------------- From michael_lafriniere <@t> memorial.org Tue Jan 27 20:34:32 2004 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER Message-ID: Hi Wayne, Currently, I would advise against separation of the two specimens received in one container and labeling received as A&B and charging two distinct separate cpt codes. I would only charge separate cpt codes if RECEIVED separately. However, this is a very interesting subject and seeing the different ways laboratories are dealing with this issue. I personally will go one step further and seek advisement from CAP and will put their advice here on the histonet as soon as I receive a response. Regards, Michael LaFriniere PA,HT(ASCP) -----Original Message----- From: Wayne Holland [mailto:wayneholland1959@msn.com] Sent: Tuesday, January 27, 2004 6:17 PM To: pruegg@colobio.com; DDittus787@aol.com; Histonet@pathology.swmed.edu Subject: RE: [Histonet] TWO SPECIMENS IN ONE CONTAINER I have a new twist to this question of two or more specimens in one container. Often we receive two or more skin lesions, but mostly skin punch biopsies in one specimen container from outside doctors offices. However; usually these specimens are labeled as the same area of excision, but obvious distinct different sites. I have expressed my concern for obvious reasons, both for the pathologist and the physician. But the main question I need answers to is, can we separate these specimens into a,b,c- etc; ??? I guess I have not looked in the right place for the answer, until now hopefully. Is it legal to separate and charge accordingly? I know we can separate and process in a number of ways, but this will not provide correct billing. Any feedback would be great. >From: "Patsy Ruegg" >To: , >Subject: RE: [Histonet] TWO SPECIMENS IN ONE CONTAINER >Date: Tue, 27 Jan 2004 14:32:09 -0700 > >Dana I can't answer your question but this reminds me of something that >really happened to me. Bare with me folks I am in a mood..... > >There was once a cytotechnologists who was not allowed to screen cytologies >anymore because they had signed out too many positive cases as negative. >The department could not get rid of this person outright (don't ask me why >not) so they made them work as a gofer for all the other labs. I was >running the heme/path lab at the time by myself and very understaffed. >Besides all the laboratory duties I had I also assisted with bone marrow >biopsies which took me out of the lab away from my duties even more. Since >the demoted cytotech had experience with assisting with FNA's etc., the >administration put them on bone marrow assistance duty to help me out. >When >word got around that this person was helping out, my pathologists came to >me >an said specifically "do not let that person touch my samples". Well, the >admin. person said send them on bm duty anyway. On a very busy day, this >person came back to my lab with one vial with 2 bone marrow biopsies in the >vial and said to me as they handed me the vial "the longer sample belongs >to >patient Joe Blow and the shorter sample belongs to patient Mary Doe". The >tech had run out of fixative vials and decided to put two samples from two >different patients in the same vial instead of coming back to the lab for >more vials. Imagine my horror! I carefully recorded the description of >which sample belonged to who so that at least that would not get lost. I >then walked the tech with the vial with 2 bm samples in it to the office of >the administrator and said that I would not take responsible for this >situation and wanted nothing to do with it. I also told the admin. what my >pathologists had just said to me about not letting that person touch their >samples. >I was told to process the samples as usual and label them as had been >described. I did. As it turned out there was no difference in diagnosis >in these two samples. They were the same. These were renal patients being >biopsied to test for aluminum bone disease and they both were negative. >Imagine if it had come out some other way. One positive and one neg. or if >they were leukemia patients and one was in remission and the other not. As >I recall it still took months to finally get rid of this person, but they >did not do bm assistant duty anymore. It matters what we do and how we do >it! >Best regards, >Patsy > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >DDittus787@aol.com >Sent: Tuesday, January 27, 2004 1:23 PM >To: Histonet@pathology.swmed.edu >Subject: [Histonet] TWO SPECIMENS IN ONE CONTAINER > > >Hi, once more i am asking for your assistance, what are you guys doing, >when >two vas or fallopian tube come in one container? as i understand it, for >compliance when one container one cassette, you cannot designate right and >left unless indicated.am i barking up the wrong tree or is this the >compliance issue so that all things are correct. help me out here. >thanks > Dana > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Check out the coupons and bargains on MSN Offers! http://shopping.msn.com/softcontent/softcontent.aspx?scmId=1418 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagermeierjp <@t> upmc.edu Tue Jan 27 22:41:09 2004 From: gagermeierjp <@t> upmc.edu (Gagermeier, James) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Ideal endothelial cell-isolatin' antibody Message-ID: <9122C182D4268F45BCB9E64A10B7331F024D126F@1upmc-msx7.isdip.upmc.edu> I am attempting to find an antibody for immunofluorescence that is the best (from experience) in isolating endothelial cells. This is significant for me as I am attempting to isolate endothelail cells in order to perform laser microdissection on these cells for further studies. The problem I am faced with is that an approach utilizing double staining will take too long, allowing RNA degradation. It is reccomended that a specific primary biotinylated antibody be used - and then a rapid IF staining kit by Arcturus is used. The IF protocol appears to take 12-15 minutes. I am trying to determine the best antibody so that I can use a single biotinylated primary antibody with the rapid Arcturus IF protocol. Any reccs are appreciated. Thanks Jim Gagermeier From JNocito <@t> Pathreflab.com Wed Jan 28 04:24:40 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Thank you Cancer Diagnostics Message-ID: I want to thank Cancer Diagnostics for not doing anything to try make amends for their inferior marking pens. Last November, I had an entire run of cassettes have their number come off during processing. Some of you may have remembered Reality Check 1 Well, I returned all 10 packages of pens with a lettering explaining the incident as well as a request for a refund. Not one person tried to contact me via phone, mail, e-mail. Nothing. I waited for the holidays to pass thinking that I would be patient waiting for a response. I've been patient too long. So if Cancer Diagnostics is listening or reading, do not bother contacting me. It's too late and I do not wish to do business with you. Thanks for nothing. Joe Nocito From lpwenk <@t> covad.net Wed Jan 28 04:31:23 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] von kossa stain References: <1c0.145a8f54.2d482295@aol.com> Message-ID: <001a01c3e589$e0a202a0$8732fea9@hppav> My understanding of the von Kossa stain is that it is NOT specific for calcium. Rather, the silver nitrate in the staining solution (silver ion is + charge) binds with the anionic (- charge) portion of the salt. As you indicated, the von Kossa usually stains calcium phosphate and calcium carbonate, by binding the positive silver ion with the negative phosphate or carbonate portion. (Not by binding with the calcium.) This then makes a different silver salt (silver phosphate or calcium phosphate, for example), which we will see as black. In theory, then, von Kossa could also then bind with any other phosphate or carbonate salt, such as sodium phosphate. In theory, these would also stain black. However, our bodies usually do not have enough of these other salts in our bodies to make a positive reaction. So, usually, when we see positive staining with von Kossa, we assume it is demonstrating a calcium salt. Also, not all calcium salts give a positive (black) reaction. Calcium oxalate is thought to not turn black when exposed to silver nitrate. Now, whether the silver does not bind with the oxalate, or whether the silver does bind with the oxalate but the new silver oxidate does not turn black, well, I don't know. So, in summary, some anions (carbonate and phosphate) do turn black when exposed to silver nitrate, while others do not (oxalate). In summary, the von Kossa stain is not specific for calcium, but is specific to the anions of different salts, but not all salts. I tried (quickly) this morning to look up the chemical formula for a tinanate, but couldn't find anything. Obviously, since the tinanate is bound to calcium, the tinanate is negatively charged. The big questions are: - will silver ions bind with the tinanate portion - will silver tinanate be created when calcium tinanate and silver nitrate are combined - does silver tinanate turn black so we can see it. Sorry, I don't know the answers to these. Hopefully, someone else will. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Tuesday, January 27, 2004 3:22 PM Subject: [Histonet] von kossa stain > hello everyone > Do you know if does von kossa stain give a positif result with any calcium > salts ? > i think the most common ions are phosphate and carbonates, but if it is > "calcium titanate" do you think if this salt is soluble and can give a positive > result with von kossa stain ? > thank you for any help > Myriam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From philip.bergin <@t> microbio.gu.se Wed Jan 28 04:29:27 2004 From: philip.bergin <@t> microbio.gu.se (Phil Bergin) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] mouse epithelium Message-ID: Hi, I have a colleague that has fed mice DAPI fluorescent particles and is interested in what cells take it up in the intestine. Does anyone have a good method for identifying the epithelial cells. She was mainly interested in a surface marker expressed on most murine epithelial cells. This isn?t my area, so any advice would be greatly appreciated! Thanks in advance, Phil ----------------------------------------------------------------- Philip Bergin G?teborg University Department of Medical Microbiology and Immunology Box 435, SE-405 30 G?teborg, Sweden Phone: +46-31-7736213 Fax: +46-31-7736205 From Michele.Ellender <@t> nrpb.org Wed Jan 28 05:21:33 2004 From: Michele.Ellender <@t> nrpb.org (Michele Ellender) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] staining for stellate cells Message-ID: <1674D52D81376743AE9AC83C6A50F0B72C6998@hilda2.inexsys.org> Hello all, does anyone have a staining method to stain for stellate cells in mouse liver sections? Thanks, Michele Dr Michele Ellender Radiation Effects Department, NRPB, Chilton, Didcot, Oxon. OX11 0RQ. UK michele.ellender@nrpb.org This e-mail transmission is strictly confidential and intended solely for the person or organisation to whom it is addressed. It may contain privileged and confidential information and if you are not the intended recipient, please do not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, please notify the sender as soon as possible and delete the message. Please note that NRPB monitors incoming and outgoing e-mail for compliance with its Acceptable Use Policy. This will include scanning incoming e-mails to detect viruses and key-words and may in some circumstances result in the manual monitoring of the content of messages. National Radiological Protection Board E-mail: nrpb@nrpb.org Web site: www.nrpb.org -------------------------------------------- From cfavara <@t> niaid.nih.gov Wed Jan 28 06:43:40 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] lectinRCA120 and Laminin Message-ID: Hi All, Thought I sent this previously but no responses. Anyone working with lectin RCA120 for microglial staining and or Laminin on formalin fixed paraffin embedded mouse tissue? Know there was some discussion about lectin staining but have not been able to find anything in the archives. Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 From siksik03 <@t> comcast.net Wed Jan 28 07:02:50 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Kryofix In-Reply-To: References: Message-ID: Hi HistoNetters Kryofix was a product of EM Sciences/Merck. I believe that it has been replaced by a new product called "NeoFix". Energy Beam Sciences also sold a replacement product developed with the assistance of Anatech called MicroFix. All of these products are ethyl alcohol/polyethylene glycol fixatives. Dr. Boon is selling a similar product in Europe called BoonFix. best regards, Steven Slap Microwave Consultant At 1:46 PM -0500 1/27/04, jo-ann wrote: >Can someone tell me if you have heard of a product called "Kryofix"? Where >can I get it in Canada? From MGINSU <@t> aol.com Wed Jan 28 07:09:56 2004 From: MGINSU <@t> aol.com (MGINSU@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Biopsy Sponges Melting in Processor Message-ID: Morning Histonetters, I was wondering if anyone has ever come across having their biopsy sponges melt during their routine processing cycle, and if so, what was the cause. We use Fisher blue biopsy pads and run a routine 12-hour overnight cycle. Any input is great appreciated. Bobby Surgical Pathology Laboratories, Florida :) From DDDeltour <@t> sig.med.navy.mil Wed Jan 28 07:35:31 2004 From: DDDeltour <@t> sig.med.navy.mil (Deltour, Douglas D.(HM2)) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Biopsy Sponges Melting in Processor Message-ID: What temp do you have your Fluids/Wax set at? HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy Supervisor Histology Histology Technician HT -----Original Message----- From: MGINSU@aol.com [mailto:MGINSU@aol.com] Sent: Wednesday, January 28, 2004 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Sponges Melting in Processor Morning Histonetters, I was wondering if anyone has ever come across having their biopsy sponges melt during their routine processing cycle, and if so, what was the cause. We use Fisher blue biopsy pads and run a routine 12-hour overnight cycle. Any input is great appreciated. Bobby Surgical Pathology Laboratories, Florida :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This document may contain information covered under the privacy Act, 5 USC 552(a), and/or the Health Insurance Portability and Accountability Act (PL 104-191) and its various implementing regulations and must be protected in accordance with those provisions. Healthcare information is personal and sensitive and must be treated accordingly. If this correspondence contains healthcare information it is being provided to you after applying the appropriate security controls and authorization from the patient, or under circumstances that don't require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Redisclosure without additional patient consent or as permitted by law is prohibited. Unauthorized redisclosure or failure to maintain confidentiality subjects you to application of appropriate sanction. If you have received this correspondence in error, please notify the sender and the command Privacy Officer at privacy@sig.med.navy.mil at once and destroy any copies you have made. From susan.wells <@t> bms.com Wed Jan 28 07:00:21 2004 From: susan.wells <@t> bms.com (Susan Q Wells) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Muscle fiber differentiation Message-ID: <4017B265.CE87E19B@bms.com> Does anyone have any ideas on how to distinguish Type 1 and Type 2 muscle fibers with IHC? We have done an ATPase stain but wanted to know if there are available antibodies? Thanks in advance for your help, Sue Wells HT(ASCP),QIHC From hhawkins <@t> utmb.edu Wed Jan 28 08:23:40 2004 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] differential stains Message-ID: <44F06D8E2C162C4F92D0C6900AC434CE2F0FD0@EXCHANGE2K3.utmb.edu> In our research on acute lung injury in sheep, we have found that partial or complete obstruction of bronchi and bronchioles is a significant problem. The obstructive material is composed (mainly) of mucus, both neutral and acidic, fibrin, and cells and cell debris including epithelial cells and neutrophils. If possible, we would like to use a histologic stain that will stain the mucins a distinct color different from the cells and the fibrin, and another stain for fibrin that will let us estimate its extent. Even better would be a stain that would mark mucus, fibrin, and cells differently on the same section. Has anyone used or developed such a combination stain? Any suggestions on how to proceed? Many thanks, Hal Hawkins UT Medical Branch, Galveston, TX, USA From browning <@t> HHSC.CA Wed Jan 28 08:29:42 2004 From: browning <@t> HHSC.CA (Browning Deb) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Biopsy Sponges Melting in Processor Message-ID: <3AADFB88753AD31189C100902786B91C0E277FC1@hch_nt_exchange.hhsc.ca> We had the same problem last year, the blue sponges disintegrated during the processing. I went thru a lengthy project with each reagent on the processor trying to find out why/what happened, with no easy answer, I couldn't duplicate the disintegration. We ended up changing every solution on the processor, and it hasnt' happened since, but it leaves you with an uneasy feeling because it wasn't answered, just momentarily resolved. Good luck. -----Original Message----- From: Deltour, Douglas D.(HM2) [mailto:DDDeltour@sig.med.navy.mil] Sent: Wednesday, January 28, 2004 8:36 AM To: 'MGINSU@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biopsy Sponges Melting in Processor What temp do you have your Fluids/Wax set at? HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy Supervisor Histology Histology Technician HT -----Original Message----- From: MGINSU@aol.com [mailto:MGINSU@aol.com] Sent: Wednesday, January 28, 2004 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Sponges Melting in Processor Morning Histonetters, I was wondering if anyone has ever come across having their biopsy sponges melt during their routine processing cycle, and if so, what was the cause. We use Fisher blue biopsy pads and run a routine 12-hour overnight cycle. Any input is great appreciated. Bobby Surgical Pathology Laboratories, Florida :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This document may contain information covered under the privacy Act, 5 USC 552(a), and/or the Health Insurance Portability and Accountability Act (PL 104-191) and its various implementing regulations and must be protected in accordance with those provisions. Healthcare information is personal and sensitive and must be treated accordingly. If this correspondence contains healthcare information it is being provided to you after applying the appropriate security controls and authorization from the patient, or under circumstances that don't require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Redisclosure without additional patient consent or as permitted by law is prohibited. Unauthorized redisclosure or failure to maintain confidentiality subjects you to application of appropriate sanction. If you have received this correspondence in error, please notify the sender and the command Privacy Officer at privacy@sig.med.navy.mil at once and destroy any copies you have made. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From Marilyn.Wadsworth <@t> uvm.edu Wed Jan 28 08:51:51 2004 From: Marilyn.Wadsworth <@t> uvm.edu (Wadsworth, Marilyn P) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Articular cartilage wrinkles Message-ID: <92FA977E2471094E8A2B699738A0C8090AD1FD@med04.med.uvm> Dear Histonet, > I am processing specimens of articular cartilage/bone from rabbit > specimens. The tissue has been fixed and decaled using EDTA in > formalin using the method of Kirviranta, 1985. > > Kiviranta, I., et al., Microspectrophotometric quantitation of > glycosaminoglycans in articular cartilage sections stained with > Safranin O. Histochemistry., 1985. 82(3): p. 249-55. > > The sections are paraffin embedded and sectioned at 5 or 6 microns. > Upon mounting on slides, the articular cartilage appears puckered or > wrinkled (result of swelling of cartilage when exposed to water). Is > there anything that may be done to eliminate this artifact? Is it > possible to float the microtomed sections on a non-aqueous solution? > I'd appreciate any tips you may offer. Thanks > Maria Roemhildt, PhD University of Vermont Department of Orthopaedics & Rehabilitation 95 Carrigan Drive Burlington, VT 05405 Ph. 802-656-3823 fax: 802-656-4247 maria.roemhildt@med.uvm.edu From djones <@t> upei.ca Wed Jan 28 07:59:07 2004 From: djones <@t> upei.ca (Darlene Jones) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Immunohistochemistry - Micobacterium Message-ID: <401787EB.5073.3F0324@localhost> I am new to the Histonet World, so hello everyone, Would anyone have any suggestions as to where I could find a source of a immunohistochemistry protocol for Micobacterium on the avian subspecies paratuberculosis? Including reagent and antibody sources? Thank-you, Darlene Jones, BSc. Necropsy & Research Technician Department of Pathology & Microbiology Atlantic Veterinary College, U.P.E.I. Phone:(902)628-4314 Fax:(902)566-0851 E-mail:djones@upei.ca From Tora.Bardal <@t> bio.ntnu.no Wed Jan 28 09:15:43 2004 From: Tora.Bardal <@t> bio.ntnu.no (Tora Bardal) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] combined ISH/IHC Message-ID: <5.2.0.9.2.20040128155027.00b64008@pop.nt.ntnu.no> Hello Does anyone have a working protocol they can share? I'm trying to combine in situ hybridization (RNAprobe) and immunohistochemistry (PCNA) on fish paraffin sections. So far: ISH ok, IHC doesn't work, or ISH no signal, IHC ok. PCNA(DAKO) needs AR. Before spending more time an money I would like to have some good advices on what to do when, ab simultaneous incubation? stepwise? crossreactions? blocking ? I'm using sheep anti-DIG-AP/BCIP/NBT (ISH) and DAKO Envision HRP (mouse)/DAB. Tora Tora Bardal Department of Biology, Norwegian University of Science and Technology (NTNU) Bratt?ra Research Center N-7491 Trondheim Norway + (47)73 59 09 38 / 970 25256 E-mail: Tora.Bardal@bio.ntnu.no fax:+ (47)73 59 63 11 From pzeitlow <@t> bbpllab.com Wed Jan 28 09:29:35 2004 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] FW: ASRs Message-ID: <813FB33DA405334F947F8BFC6EBD0B2AE652@bbplsrv1.bbpl> This discussion takes place frequently and I also would like final resolution. The following statement is from CAP Advocacy (11/98) and seems clear enough. Please let me know if something more current exists. ASR FACT SHEET "The FDA very clearly distinguishes ASRs from Immunohistochemistry (IHC) reagents (final rule effective Aug. 17, 1998). The FDA defines IHCs as "in vitro diagnostic devices...intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASRs, IHCs are labeled by manufacturers with directions for use and performance indications." Based upon that, my interpretation and practice has been that disclaimers are unnecessary on IHC antibodies in general. It may be that you could be using antibodies that are worked up with home brew methods (do not come with instructions and performance indications) in which case I would assume the disclaimer would be appropriate. I look forward to the responses from the group on this. Pat Zeitlow Boyce & Bynum Laboratories -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, January 27, 2004 1:43 PM To: 'Mitchell, Jeannette M.'; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs The disclaimer is necessary if the antibody is labeled ASR by the manufacturer. If it is, you must use the disclaimer. Almost all of our antibodies are IVD or ASR and we bill for those. But, if we have a RUO antibody (we have 2) we do not bill for those immunos. It's not a CAP requirement, it's a CLIA requirement for the statement and CAP is just following through with their inspections to see if labs are complying. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org] Sent: Tuesday, January 27, 2004 1:05 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] FW: ASRs > Can folks tell me what they are doing with regard to CAP Checklist > Dec. 2003 question # ANP.12425 regarding ASRs (Analyte Specific > Reagents) ? We currently do not have any disclaimer in our patient > surgical reports because all our antibodies are commercially produced > (not "home brews"). In reading this recently revised requirement > however it sounds like we need to have this statement in all our > reports that have had IHC performed. Most of the antibodies we use > have package inserts saying for "Research Use Only". > > I'd appreciate any feedback as to whether the CAP recommended > disclaimer is necessary with commercially bought antibodies (not in > kits). thanks Jude > Jude Carpenter, BS, HTL(ASCP) > Chief Technologist for Autopsy/Histology/Surgical Pathology > 111 Colchester Ave. > Burlington, VT 05401 > jude.carpenter@vtmednet.org > (802)847-5116 > fax: (802)847-3509 > Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Jan 28 09:36:13 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] re Laminin staining Message-ID: <002d01c3e5b4$76079bf0$e8345c9f@Carlos> I also thought I replied....ah, well. Yes I use anti Laminin on FFPP mouse tissues. Sigma L9393. Needs Ag unmasking :citric pH6 doesn't work for me: I use a TRIS soln adjusted to pH10. Lovely! ( or use trypsin): From mcauliff <@t> umdnj.edu Wed Jan 28 09:40:00 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] staining for stellate cells In-Reply-To: <1674D52D81376743AE9AC83C6A50F0B72C6998@hilda2.inexsys.org> References: <1674D52D81376743AE9AC83C6A50F0B72C6998@hilda2.inexsys.org> Message-ID: <4017D7D0.2040904@umdnj.edu> By stellate cells do you mean Kupffer cells, the fixed macrophages in the sinusoids? If so, any antibody that stains macrophages will work. For mouse Mac1 or F4/80 will work on frozen sections of fixed tissue. Geoff Michele Ellender wrote: >Hello all, >does anyone have a staining method to stain for stellate cells in mouse >liver sections? >Thanks, >Michele > >Dr Michele Ellender >Radiation Effects Department, >NRPB, Chilton, Didcot, >Oxon. OX11 0RQ. UK > >michele.ellender@nrpb.org > > > >This e-mail transmission is strictly confidential and intended >solely for the person or organisation to whom it is >addressed. It may contain privileged and confidential >information and if you are not the intended recipient, >please do not copy, distribute or take any action in >reliance on it. If you have received this e-mail in error, >please notify the sender as soon as possible and delete >the message. > >Please note that NRPB monitors incoming and outgoing >e-mail for compliance with its Acceptable Use Policy. This >will include scanning incoming e-mails to detect viruses >and key-words and may in some circumstances result in >the manual monitoring of the content of messages. > >National Radiological Protection Board >E-mail: nrpb@nrpb.org >Web site: www.nrpb.org >-------------------------------------------- > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From carl.hobbs <@t> kcl.ac.uk Wed Jan 28 09:44:27 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] re muscle fibre-typing Message-ID: <003301c3e5b5$9c623930$e8345c9f@Carlos> On FFPP mouse muscles: I used Sigma MY32 ( MHC Type II) . For me, it works without Ag unmasking, but patchy. With citric pH6 unnmasking got a paler result but not patchy. TRIS pH 10 solution may well be better. For type I MHC, I used MAB1628, from Chemicon. Again plus Citric pH6 unmasking( can try TRIS pH10 ) From Terry.Marshall <@t> rothgen.nhs.uk Wed Jan 28 10:08:19 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] staining for stellate cells Message-ID: No, I think the stellate cell is the lipocyte, or cell of Ito, which lies within the space of Disse. I know of nothing but a fat stain to demonstrate, and as always that would show the fat not the cell. For everyone's information, they become enlarged (and store the vitamin) in Vitamin A poisoning. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: 28 January 2004 15:40 To: Michele Ellender Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] staining for stellate cells By stellate cells do you mean Kupffer cells, the fixed macrophages in the sinusoids? If so, any antibody that stains macrophages will work. For mouse Mac1 or F4/80 will work on frozen sections of fixed tissue. Geoff Michele Ellender wrote: >Hello all, >does anyone have a staining method to stain for stellate cells in mouse >liver sections? >Thanks, >Michele > >Dr Michele Ellender >Radiation Effects Department, >NRPB, Chilton, Didcot, >Oxon. OX11 0RQ. UK > >michele.ellender@nrpb.org > > > >This e-mail transmission is strictly confidential and intended >solely for the person or organisation to whom it is >addressed. It may contain privileged and confidential >information and if you are not the intended recipient, >please do not copy, distribute or take any action in >reliance on it. If you have received this e-mail in error, >please notify the sender as soon as possible and delete >the message. > >Please note that NRPB monitors incoming and outgoing >e-mail for compliance with its Acceptable Use Policy. This >will include scanning incoming e-mails to detect viruses >and key-words and may in some circumstances result in >the manual monitoring of the content of messages. > >National Radiological Protection Board >E-mail: nrpb@nrpb.org >Web site: www.nrpb.org >-------------------------------------------- > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Jan 28 11:12:56 2004 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] staining for stellate cells Message-ID: .....and if you eat polar bear liver, the vitamin A will kill you! E.S. Kimo -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 28 January 2004 16:08 To: Geoff McAuliffe; Michele Ellender Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] staining for stellate cells No, I think the stellate cell is the lipocyte, or cell of Ito, which lies within the space of Disse. I know of nothing but a fat stain to demonstrate, and as always that would show the fat not the cell. For everyone's information, they become enlarged (and store the vitamin) in Vitamin A poisoning. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: 28 January 2004 15:40 To: Michele Ellender Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] staining for stellate cells By stellate cells do you mean Kupffer cells, the fixed macrophages in the sinusoids? If so, any antibody that stains macrophages will work. For mouse Mac1 or F4/80 will work on frozen sections of fixed tissue. Geoff Michele Ellender wrote: >Hello all, >does anyone have a staining method to stain for stellate cells in mouse >liver sections? >Thanks, >Michele > >Dr Michele Ellender >Radiation Effects Department, >NRPB, Chilton, Didcot, >Oxon. OX11 0RQ. UK > >michele.ellender@nrpb.org > > > >This e-mail transmission is strictly confidential and intended >solely for the person or organisation to whom it is >addressed. It may contain privileged and confidential >information and if you are not the intended recipient, >please do not copy, distribute or take any action in >reliance on it. If you have received this e-mail in error, >please notify the sender as soon as possible and delete >the message. > >Please note that NRPB monitors incoming and outgoing >e-mail for compliance with its Acceptable Use Policy. This >will include scanning incoming e-mails to detect viruses >and key-words and may in some circumstances result in >the manual monitoring of the content of messages. > >National Radiological Protection Board >E-mail: nrpb@nrpb.org >Web site: www.nrpb.org >-------------------------------------------- > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Wed Jan 28 11:40:08 2004 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Biopsy Sponges Melting in Processor Message-ID: We have had this problem when people accidentally left the blue sponges in cassettes that were put through RDO solution for decalcification and then put on the processor...but not at any other time... Vicki Gauch AMCH Albany, NY >>> Browning Deb 1/28/2004 9:29:42 AM >>> We had the same problem last year, the blue sponges disintegrated during the processing. I went thru a lengthy project with each reagent on the processor trying to find out why/what happened, with no easy answer, I couldn't duplicate the disintegration. We ended up changing every solution on the processor, and it hasnt' happened since, but it leaves you with an uneasy feeling because it wasn't answered, just momentarily resolved. Good luck. -----Original Message----- From: Deltour, Douglas D.(HM2) [mailto:DDDeltour@sig.med.navy.mil] Sent: Wednesday, January 28, 2004 8:36 AM To: 'MGINSU@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biopsy Sponges Melting in Processor What temp do you have your Fluids/Wax set at? HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy Supervisor Histology Histology Technician HT -----Original Message----- From: MGINSU@aol.com [mailto:MGINSU@aol.com] Sent: Wednesday, January 28, 2004 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biopsy Sponges Melting in Processor Morning Histonetters, I was wondering if anyone has ever come across having their biopsy sponges melt during their routine processing cycle, and if so, what was the cause. We use Fisher blue biopsy pads and run a routine 12-hour overnight cycle. Any input is great appreciated. Bobby Surgical Pathology Laboratories, Florida :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This document may contain information covered under the privacy Act, 5 USC 552(a), and/or the Health Insurance Portability and Accountability Act (PL 104-191) and its various implementing regulations and must be protected in accordance with those provisions. Healthcare information is personal and sensitive and must be treated accordingly. If this correspondence contains healthcare information it is being provided to you after applying the appropriate security controls and authorization from the patient, or under circumstances that don't require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Redisclosure without additional patient consent or as permitted by law is prohibited. Unauthorized redisclosure or failure to maintain confidentiality subjects you to application of appropriate sanction. If you have received this correspondence in error, please notify the sender and the command Privacy Officer at privacy@sig.med.navy.mil at once and destroy any copies you have made. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Wed Jan 28 11:54:55 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] FW: ASRs Message-ID: I have found a few links regarding ASR's, RUO's and what has happened in the USA with these rules. http://www.ashi-hla.org/usefullinksfiles/asr-freg.pdf This one is the FDA ruling regarding ASR's. Especially read Comment 16 on page 8. http://www.fda.gov/cdrh/comp/fr01598b.html http://www.gklaw.com/powerpoint/IP/Analyte_Specific_Reagent_Regulations. ppt The last one is a PowerPoint presentation prepared by a PhD and an attorney on the subject. If you download it and then open it in PowerPoint viewing the presenter's notes, it has a lot of information. I admit that I'm still trying to figure it out. I hope someone here knows for certain. As far as I can tell, the FDA does not want Pathologist's to use RUO's in their diagnosis, but has not begun to enforce this and may not have published final rules stating this. Please feel free to read the above links and disagree. I'm not an expert. I'm trying to figure this out myself. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Wednesday, January 28, 2004 9:30 AM To: Horn, Hazel V; Mitchell, Jeannette M.; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs This discussion takes place frequently and I also would like final resolution. The following statement is from CAP Advocacy (11/98) and seems clear enough. Please let me know if something more current exists. ASR FACT SHEET "The FDA very clearly distinguishes ASRs from Immunohistochemistry (IHC) reagents (final rule effective Aug. 17, 1998). The FDA defines IHCs as "in vitro diagnostic devices...intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASRs, IHCs are labeled by manufacturers with directions for use and performance indications." Based upon that, my interpretation and practice has been that disclaimers are unnecessary on IHC antibodies in general. It may be that you could be using antibodies that are worked up with home brew methods (do not come with instructions and performance indications) in which case I would assume the disclaimer would be appropriate. I look forward to the responses from the group on this. Pat Zeitlow Boyce & Bynum Laboratories -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, January 27, 2004 1:43 PM To: 'Mitchell, Jeannette M.'; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs The disclaimer is necessary if the antibody is labeled ASR by the manufacturer. If it is, you must use the disclaimer. Almost all of our antibodies are IVD or ASR and we bill for those. But, if we have a RUO antibody (we have 2) we do not bill for those immunos. It's not a CAP requirement, it's a CLIA requirement for the statement and CAP is just following through with their inspections to see if labs are complying. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org] Sent: Tuesday, January 27, 2004 1:05 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] FW: ASRs > Can folks tell me what they are doing with regard to CAP Checklist > Dec. 2003 question # ANP.12425 regarding ASRs (Analyte Specific > Reagents) ? We currently do not have any disclaimer in our patient > surgical reports because all our antibodies are commercially produced > (not "home brews"). In reading this recently revised requirement > however it sounds like we need to have this statement in all our > reports that have had IHC performed. Most of the antibodies we use > have package inserts saying for "Research Use Only". > > I'd appreciate any feedback as to whether the CAP recommended > disclaimer is necessary with commercially bought antibodies (not in > kits). thanks Jude > Jude Carpenter, BS, HTL(ASCP) > Chief Technologist for Autopsy/Histology/Surgical Pathology > 111 Colchester Ave. > Burlington, VT 05401 > jude.carpenter@vtmednet.org > (802)847-5116 > fax: (802)847-3509 > Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From RossS <@t> BaylorHealth.edu Wed Jan 28 12:15:53 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] FW: ASRs Message-ID: I found another link which has the FDA Draft Compliance Policy Guide. http://www.fda.gov/cdrh/comp/ivddrfg.html Important quote: "Devices labeled "For Research Use" are mislabeled if the device is being used for investigational purposes, i.e., in a clinical study, even if involving only one subject, where the diagnostic or prognostic measurement will be reported to the patient's physician or medical records or will be used to assess the patient's condition, regardless of whether or not confirmatory tests or procedures are used. Research use is limited to the initial research phase of product development that is necessary to identify test kit methods, components, and analytes to be measured or to laboratory research that is entirely unrelated to product development. See 21 CFR 809.10(c)(2)(I). However, the Agency recognizes that certain improperly commercialized IVD's have been in extensive clinical use for a significant period of time. The Agency further recognizes that immediate regulatory action against certain of these IVD's might result in adverse consequences to individual patients and the public health. Therefore, FDA is publishing this CPG in order to describe the Agency's enforcement policy, which includes the Agency's intention to exercise discretion for designated periods of time, so as not to cause undue disruption to the possibly beneficial use of IVD's that have not received Agency clearance prior to commercialization." This is from 1998 and is a draft to get public comments prior to issuing a final Compliance Policy Guide. I can't find where a final CPG was ever released. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stapf, Ross Sent: Wednesday, January 28, 2004 11:55 AM To: Horn, Hazel V; Mitchell, Jeannette M.; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs I have found a few links regarding ASR's, RUO's and what has happened in the USA with these rules. http://www.ashi-hla.org/usefullinksfiles/asr-freg.pdf This one is the FDA ruling regarding ASR's. Especially read Comment 16 on page 8. http://www.fda.gov/cdrh/comp/fr01598b.html http://www.gklaw.com/powerpoint/IP/Analyte_Specific_Reagent_Regulations. ppt The last one is a PowerPoint presentation prepared by a PhD and an attorney on the subject. If you download it and then open it in PowerPoint viewing the presenter's notes, it has a lot of information. I admit that I'm still trying to figure it out. I hope someone here knows for certain. As far as I can tell, the FDA does not want Pathologist's to use RUO's in their diagnosis, but has not begun to enforce this and may not have published final rules stating this. Please feel free to read the above links and disagree. I'm not an expert. I'm trying to figure this out myself. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Wednesday, January 28, 2004 9:30 AM To: Horn, Hazel V; Mitchell, Jeannette M.; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs This discussion takes place frequently and I also would like final resolution. The following statement is from CAP Advocacy (11/98) and seems clear enough. Please let me know if something more current exists. ASR FACT SHEET "The FDA very clearly distinguishes ASRs from Immunohistochemistry (IHC) reagents (final rule effective Aug. 17, 1998). The FDA defines IHCs as "in vitro diagnostic devices...intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASRs, IHCs are labeled by manufacturers with directions for use and performance indications." Based upon that, my interpretation and practice has been that disclaimers are unnecessary on IHC antibodies in general. It may be that you could be using antibodies that are worked up with home brew methods (do not come with instructions and performance indications) in which case I would assume the disclaimer would be appropriate. I look forward to the responses from the group on this. Pat Zeitlow Boyce & Bynum Laboratories -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, January 27, 2004 1:43 PM To: 'Mitchell, Jeannette M.'; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs The disclaimer is necessary if the antibody is labeled ASR by the manufacturer. If it is, you must use the disclaimer. Almost all of our antibodies are IVD or ASR and we bill for those. But, if we have a RUO antibody (we have 2) we do not bill for those immunos. It's not a CAP requirement, it's a CLIA requirement for the statement and CAP is just following through with their inspections to see if labs are complying. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org] Sent: Tuesday, January 27, 2004 1:05 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] FW: ASRs > Can folks tell me what they are doing with regard to CAP Checklist > Dec. 2003 question # ANP.12425 regarding ASRs (Analyte Specific > Reagents) ? We currently do not have any disclaimer in our patient > surgical reports because all our antibodies are commercially produced > (not "home brews"). In reading this recently revised requirement > however it sounds like we need to have this statement in all our > reports that have had IHC performed. Most of the antibodies we use > have package inserts saying for "Research Use Only". > > I'd appreciate any feedback as to whether the CAP recommended > disclaimer is necessary with commercially bought antibodies (not in > kits). thanks Jude > Jude Carpenter, BS, HTL(ASCP) > Chief Technologist for Autopsy/Histology/Surgical Pathology 111 > Colchester Ave. Burlington, VT 05401 > jude.carpenter@vtmednet.org > (802)847-5116 > fax: (802)847-3509 > Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From JosefaNava <@t> texashealth.org Wed Jan 28 13:34:34 2004 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] looking for antibodies :HPL,EBV,AE1 AE3, BER-e P04 Message-ID: <7A5E02BB0E0A2E409F971D889B200C298A53D2@phdex05.txhealth.org> Hello to Everyone! I am looking for antibodies that will work well with our Ventana Benchmark . The antibodies are HPL, AE1 AE3, EBV and BER-eP04. Thank you. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From janis-rodgers <@t> uiowa.edu Wed Jan 28 13:58:51 2004 From: janis-rodgers <@t> uiowa.edu (Rodgers, Janis) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] caspase 2 Message-ID: <5D03ED7B9391D4119D9B0008C76B7B24062B0E8E@uihc-mail1.uihc.uiowa.edu> Has anyone had success with IHC for caspase 2 on FFPE tissue? Thanks Jan Rodgers From RSRICHMOND <@t> aol.com Wed Jan 28 14:13:28 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Re: TWO SPECIMENS IN ONE CONTAINER Message-ID: <12d.39dfe974.2d4971e8@aol.com> Dana Dittus asks the old, eternally confusing question about what to do with two specimens in the same container. If it's possible to tell which specimen is which, it's two specimens, and the case will get two CPT codes (thus tube segments would be 88302 x2, or two skin bumps would be 88305 x2. One of the tube segments might be marked with a suture, or one of the skin bumps might be described by the submitting surgeon as larger than the other, or three skin bumps might be strung on a needle with the note that the one closest to the needle hub is from the left elbow, the one in the middle is from the right ankle, and the one closest to the point of the needle is from the ol' wazoo. (I've seen all of these methods used.) If there is no way to tell the specimens apart, then only a single CPT code can be used. It doesn't matter whether the specimen is embedded with one cassette or two, as long as the specimens can be told apart (say by inking one of them). (This is how it's done in the USA - other countries could have wholly different rules.) Bob Richmond Samurai Pathologist P.S. The ol' Samurai Pathologist has a FULL TIME JOB in Gastonia NC (west of Charlotte) with a group of five pathologists at a 450 bed hospital. Superb histology and other laboratory services. - Same e-mail address and all. From tpmorken <@t> labvision.com Wed Jan 28 15:06:16 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] FW: ASRs Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20357D6@usca0082k08.labvision.apogent.com> CellMarque has a concise explanation of the IVD/ASR system at http://www.cellmarque.com/2000/Pages/finalrule.html Also, As I understand it, an RUO antibody can be used like an ASR if the institution undertakes full validation (that includes proving the intended target is labeled). There are some large institutions that develop their own antibodies in a research setting and then validate them for in-house diagnostic use. Tim Morken Lab Vision / Neomarkers www.labvision.com -----Original Message----- From: Stapf, Ross [mailto:RossS@BaylorHealth.edu] Sent: Wednesday, January 28, 2004 9:55 AM To: Horn, Hazel V; Mitchell, Jeannette M.; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs I have found a few links regarding ASR's, RUO's and what has happened in the USA with these rules. http://www.ashi-hla.org/usefullinksfiles/asr-freg.pdf This one is the FDA ruling regarding ASR's. Especially read Comment 16 on page 8. http://www.fda.gov/cdrh/comp/fr01598b.html http://www.gklaw.com/powerpoint/IP/Analyte_Specific_Reagent_Regulations. ppt The last one is a PowerPoint presentation prepared by a PhD and an attorney on the subject. If you download it and then open it in PowerPoint viewing the presenter's notes, it has a lot of information. I admit that I'm still trying to figure it out. I hope someone here knows for certain. As far as I can tell, the FDA does not want Pathologist's to use RUO's in their diagnosis, but has not begun to enforce this and may not have published final rules stating this. Please feel free to read the above links and disagree. I'm not an expert. I'm trying to figure this out myself. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Wednesday, January 28, 2004 9:30 AM To: Horn, Hazel V; Mitchell, Jeannette M.; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs This discussion takes place frequently and I also would like final resolution. The following statement is from CAP Advocacy (11/98) and seems clear enough. Please let me know if something more current exists. ASR FACT SHEET "The FDA very clearly distinguishes ASRs from Immunohistochemistry (IHC) reagents (final rule effective Aug. 17, 1998). The FDA defines IHCs as "in vitro diagnostic devices...intended to identify, by immunological techniques, antigens in tissue or cytologic specimens." Unlike ASRs, IHCs are labeled by manufacturers with directions for use and performance indications." Based upon that, my interpretation and practice has been that disclaimers are unnecessary on IHC antibodies in general. It may be that you could be using antibodies that are worked up with home brew methods (do not come with instructions and performance indications) in which case I would assume the disclaimer would be appropriate. I look forward to the responses from the group on this. Pat Zeitlow Boyce & Bynum Laboratories -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, January 27, 2004 1:43 PM To: 'Mitchell, Jeannette M.'; Histonet@pathology.swmed.edu Subject: RE: [Histonet] FW: ASRs The disclaimer is necessary if the antibody is labeled ASR by the manufacturer. If it is, you must use the disclaimer. Almost all of our antibodies are IVD or ASR and we bill for those. But, if we have a RUO antibody (we have 2) we do not bill for those immunos. It's not a CAP requirement, it's a CLIA requirement for the statement and CAP is just following through with their inspections to see if labs are complying. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org] Sent: Tuesday, January 27, 2004 1:05 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] FW: ASRs > Can folks tell me what they are doing with regard to CAP Checklist > Dec. 2003 question # ANP.12425 regarding ASRs (Analyte Specific > Reagents) ? We currently do not have any disclaimer in our patient > surgical reports because all our antibodies are commercially produced > (not "home brews"). In reading this recently revised requirement > however it sounds like we need to have this statement in all our > reports that have had IHC performed. Most of the antibodies we use > have package inserts saying for "Research Use Only". > > I'd appreciate any feedback as to whether the CAP recommended > disclaimer is necessary with commercially bought antibodies (not in > kits). thanks Jude > Jude Carpenter, BS, HTL(ASCP) > Chief Technologist for Autopsy/Histology/Surgical Pathology 111 > Colchester Ave. Burlington, VT 05401 > jude.carpenter@vtmednet.org > (802)847-5116 > fax: (802)847-3509 > Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kosmicdog <@t> hotmail.com Wed Jan 28 16:07:32 2004 From: kosmicdog <@t> hotmail.com (jason madore) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] cell pellets or spheriods - FFPE protocol? Message-ID: First, thank you everyone for all the help from this site. A great resource and obviously a great bunch of people. Does anyone have a bench top protocol or advice for putting cell pellets or spheriods (hanging cell droplets) in formalin and embedding in paraffin. Keep in mind that this is not a pathology lab and I do not have access to much fancy equipment. Thanks in advance. Jason. Research Assistant Institut du cancer de Montréal Y4609-Pavillon J.A. De Seve 2099, rue Alexandre De Seve Montréal, Quebec Canada. H2L 2W5 tele: 514.890.8000e27082 _________________________________________________________________ The new MSN 8: smart spam protection and 2 months FREE* http://join.msn.com/?page=features/junkmail http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From Barry.R.Rittman <@t> uth.tmc.edu Wed Jan 28 16:25:14 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Articular cartilage wrinkles Message-ID: <566FB0B522443D43AF02D2ADBE35A6F063592C@UTHEVS3.mail.uthouston.edu> Hi Maria Because of uneven shrinkage and the different rates at which tissues dry on a warming plate, you will generally get some wrinkling ofparaffin sections. What we used to do for cartilage, bone and teeth was to brush out the wrinkles. (No I have not been at the Bourbon!) Place the slide with most of the water drained on a slide warming plate. Make sure that the paraffin wax is not going to restrict the section in the direction you are going to brush and that you do not have paraffin smears as these will prevent the tissue from moving in that direction. Using a fine sable brush gentle brush out the wrinkles. This is a skill that needs to be acquired and depends on care in selecting the direction of brushing and brushing out those tissues that dry fastest cartilage, then bone, so you may screw up a few sections first. After brushing out a small portion of the section, dry the brush by briefly touching to some filter paper. The important thing is to go slowly and select the best direction to brush. You are in effect gradually pushing the water from under the section and at the same time are flattening the section, removing the folds. This is superior to using needles as it will not cause tears in the tissue. If you have not managed to brush out a section before it starts to dry to much simply add a little fluid to the fold and then try again. You have to be careful not to brush the soft tissue as you will generally damage this. Once section has been brushed and is dry then gently pass over a spirit lamp to JUST melt the wax. This will aid in keeping the section on the slide. Two points There are sometimes some folds that will simply not be removable and you have to accept that. Second is that if you have fluid on top of the section you will not be able to remove folds from that area unless you can remove that fluid. For those of you who are new to histotechnology, this method was suggested by a trainee only two weeks after they started working with us! Call me if you need any further details. Barry 713-500-4134 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wadsworth, Marilyn P Sent: Wednesday, January 28, 2004 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Articular cartilage wrinkles Dear Histonet, > I am processing specimens of articular cartilage/bone from rabbit > specimens. The tissue has been fixed and decaled using EDTA in > formalin using the method of Kirviranta, 1985. > > Kiviranta, I., et al., Microspectrophotometric quantitation of > glycosaminoglycans in articular cartilage sections stained with > Safranin O. Histochemistry., 1985. 82(3): p. 249-55. > > The sections are paraffin embedded and sectioned at 5 or 6 microns. > Upon mounting on slides, the articular cartilage appears puckered or > wrinkled (result of swelling of cartilage when exposed to water). Is > there anything that may be done to eliminate this artifact? Is it > possible to float the microtomed sections on a non-aqueous solution? > I'd appreciate any tips you may offer. Thanks > Maria Roemhildt, PhD University of Vermont Department of Orthopaedics & Rehabilitation 95 Carrigan Drive Burlington, VT 05405 Ph. 802-656-3823 fax: 802-656-4247 maria.roemhildt@med.uvm.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bryand <@t> netbistro.com Wed Jan 28 18:18:30 2004 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] differential stains References: <44F06D8E2C162C4F92D0C6900AC434CE2F0FD0@EXCHANGE2K3.utmb.edu> Message-ID: <01b601c3e5fd$ac4cb260$a270c2cf@bryand> Try Garvey's modification of the Movat pentachrome stain. http://members.pgonline.com/~bryand/StainsFile/stain/many/garvey_movat.htm Alternatively, do a standard alcian blue and counterstain with the MSB. Bryan Llewellyn ----- Original Message ----- From: "Hawkins, Hal K." To: "histonet" Sent: Wednesday, January 28, 2004 6:23 AM Subject: [Histonet] differential stains In our research on acute lung injury in sheep, we have found that partial or complete obstruction of bronchi and bronchioles is a significant problem. The obstructive material is composed (mainly) of mucus, both neutral and acidic, fibrin, and cells and cell debris including epithelial cells and neutrophils. If possible, we would like to use a histologic stain that will stain the mucins a distinct color different from the cells and the fibrin, and another stain for fibrin that will let us estimate its extent. Even better would be a stain that would mark mucus, fibrin, and cells differently on the same section. Has anyone used or developed such a combination stain? Any suggestions on how to proceed? Many thanks, Hal Hawkins UT Medical Branch, Galveston, TX, USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Jan 28 18:21:09 2004 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] (no subject) Message-ID: <494304423C63E246A5CF87A3AEEB577011EDF4@bumail01.barrynet.barry.edu> Fontana's silver stain will show serotonin-secreting cells, but it probably shows histamine secreting cells, too. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne-Sophie Martinez Sent: Thursday, January 22, 2004 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello everybody, Can I localize the intestinal cells which produce the serotonin, not with an antibody against this hormone, but with a special histological staining? Thank you very much for your help. AS ---------------------------------------------- Dr Anne-Sophie Martinez School of Biology University of St. Andrews Bute Medical Buildings St. Andrews Fife, KY16 9TS UK. Tel + 44 (0) 1334 463546 Fax + 44 (0) 1334 463600 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From murmandamus <@t> iinet.net.au Thu Jan 29 02:47:12 2004 From: murmandamus <@t> iinet.net.au (Jeff Leaming) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Biopsy Sponges Melting in Processor References: Message-ID: <001f01c3e644$7d57b350$f901a8c0@FRED> If the sponges are left in 5% nitric overnight they will turn to mush after processing..... Jeff Leaming Scientific Officer Sydney Skin Pathology ----- Original Message ----- From: To: Sent: Thursday, January 29, 2004 12:09 AM Subject: [Histonet] Biopsy Sponges Melting in Processor > Morning Histonetters, > > I was wondering if anyone has ever come across having their biopsy sponges > melt during their routine processing cycle, and if so, what was the cause. We > use Fisher blue biopsy pads and run a routine 12-hour overnight cycle. Any input > is great appreciated. > > Bobby > Surgical Pathology Laboratories, Florida :) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ernestinemiddleton <@t> yahoo.ca Thu Jan 29 04:43:25 2004 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Temporary position Message-ID: <20040129104325.77718.qmail@web41610.mail.yahoo.com> The Histology Laboratory at Montefiore Med. Center has a temp. position opening for 4 months. The position is located in grossing laboratory; hour schedule is 8-4 (Mon.- Fri.). Please contact Ernestine at 718-920-4157. Ernestine Middleton, Manager Montefiore Med. Center Bronx, NY 718-920-4157 718-547-1920 (fax) --------------------------------- Post your free ad now! Yahoo! Canada Personals From kappeler <@t> patho.unibe.ch Thu Jan 29 04:58:06 2004 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Muscle fiber differentiation References: <4017B265.CE87E19B@bms.com> Message-ID: <005201c3e656$c68bc310$27955c82@patho.unibe.ch> Hi Sue We routinely use the following mouse monoclonal antibodies on FFPE human skeletal muscle: Myosin I (slow), clone NOQ7.5.4D, Sigma M-8421; working conc. 5 ug Ig/ml, HIER in 10 mM citrate buffer, pH 6.0, pressure cooker. Myosin II (fast), clone MY-31, Sigma M.4276; working conc. 10 ug Ig/ml, HIER as above. Visualization is with a StrABC system. Hope this helps. Andi Kappeler Insitute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Susan Q Wells" To: Sent: Wednesday, January 28, 2004 2:00 PM Subject: [Histonet] Muscle fiber differentiation > Does anyone have any ideas on how to distinguish Type 1 and Type > 2 muscle fibers with IHC? We have done an ATPase stain but > wanted to know if there are available antibodies? > Thanks in advance for your help, > Sue Wells HT(ASCP),QIHC > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mwhitesi <@t> adventisthealthcare.com Thu Jan 29 08:00:19 2004 From: mwhitesi <@t> adventisthealthcare.com (Marnie Whiteside) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] ASR Validation Message-ID: Does anyone have a protocol for validating ASR (Analyte Specific Reagents) and Research Use only antibodies? Any help would be greatly appreciated. Thank you Marnie Whiteside Washington Adventist Hospital From CaltonE <@t> HSS.EDU Thu Jan 29 08:41:13 2004 From: CaltonE <@t> HSS.EDU (Calton, Ericka) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] hydroxyproline assay kit Message-ID: <1AED92C7EB53D940AAAD1D5F0D5B9E5B711FF2@hssmail2.hssmain.org> Hi. I am planning an experiment that involves quantification of collagen and proteoglycan (glycosaminoglycan) content in cartilage. Does anyone know of any assy kits for hydroxyproline or for using dimethylmethylene blue (DMMB)? Thanks. Ericka From mark.lewis <@t> thermo.com Thu Jan 29 09:31:36 2004 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: Hello everyone ! I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs. Thanks ! Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com From MAUGER <@t> email.chop.edu Thu Jan 29 09:40:17 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: We use Harris and Gills hematoxylin. >>> 01/29/04 10:31AM >>> Hello everyone ! I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs. Thanks ! Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Jan 29 09:47:24 2004 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Haematoxylin. Message-ID: <6.0.1.1.2.20040129154607.0330d050@udcf.gla.ac.uk> Mayer. Ehrlich Carazzi Heidenhain Weigert Gill. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From Lynne.Bell <@t> hitchcock.org Thu Jan 29 10:05:40 2004 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: We use Harris for histology and Gill 1 for cytology. Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From Marion.Hiles <@t> north-bristol.swest.nhs.uk Thu Jan 29 10:14:03 2004 From: Marion.Hiles <@t> north-bristol.swest.nhs.uk (Marion Hiles) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Muscle fiber differentiation Message-ID: <2EE924DF60902943AC6E2EF35155451F19CCF3@nbfexch03.north-bristol.nhs> We use Myosin Slow & Myosin Fast from Novocastra which work best on frozen sections. Some degree of staining on FFPE after microwaving in citrate pH6 buffer. Bob Quilty Dept of Neuropathology Frenchay Hospital Bristol UK -----Original Message----- From: Susan Q Wells [mailto:susan.wells@bms.com] Sent: 28 January 2004 13:00 To: histonet@pathology.swmed.edu Subject: [Histonet] Muscle fiber differentiation Does anyone have any ideas on how to distinguish Type 1 and Type 2 muscle fibers with IHC? We have done an ATPase stain but wanted to know if there are available antibodies? Thanks in advance for your help, Sue Wells HT(ASCP),QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ This e-mail is confidential and privileged. If you are not the intended recipient, please accept our apologies. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents, to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. North Bristol NHS Trust does not guarantee that this material is free from viruses or any other defects, although due care has been taken to minimise the risk. No contracts may be concluded on behalf of North Bristol NHS Trust by means of email communications. Thank you for your cooperation. ************************************************************************ From TJJ <@t> Stowers-Institute.org Thu Jan 29 10:47:24 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Optical Projection Tomography (OPT) Message-ID: Dear colleagues, Is anyone out there using the OPT technology? We have the system here and I need some pointers on making the LMP agarose that the samples are embedded in. Thanks for your help! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From BRobert <@t> ameripath.com Thu Jan 29 11:29:07 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: We use Gills III for histo and Gills I for cyto. BR -----Original Message----- From: mark.lewis@thermo.com [mailto:mark.lewis@thermo.com] Sent: Thursday, January 29, 2004 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin Hello everyone ! I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs. Thanks ! Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gsennello <@t> osip.com Thu Jan 29 11:37:30 2004 From: gsennello <@t> osip.com (Sennello, Gina) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] phospho-specific antibodies Message-ID: I was wondering how everyone was fixing tissue (xenograft tumors) for detection of phospho-specific antibodies such as pcKit, pAKT, pKDR and others? Has anyone found that cold fixatives stop the de-phosphorylation process better than room temp? We have seen a staining pattern that suggests that something is going on with either the penetration of the fixative or the phosphorylation process upon removal of the tumor. Any suggestions would be welcome. Thanks in advance, Gina Sennello Associate Scientist/ Histotechnologist OSIP 2860 Wilderness Place Boulder, CO 80301 phone 303-546-7739 fax 303-444-0672 From NSEARCY <@t> swmail.sw.org Thu Jan 29 11:37:05 2004 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Need your help Message-ID: <04Jan29.113726cst.119126@healthcare2.sw.org> Have been asked to research surgeries that are happening frequently that pathologist believe that "are not standard-of-care" and could possibly be a research project or not necessary to procedure. Where would I go for help with the matter? Can-o-worms. A pathologist asked me to research??? Many thanks From garygill <@t> dcla.com Thu Jan 29 11:45:24 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: I recommend Gill's for everything. ;-) Gary Gill -----Original Message----- From: BRobert@ameripath.com [mailto:BRobert@ameripath.com] Sent: Thursday, January 29, 2004 12:29 PM To: mark.lewis@thermo.com; histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hematoxylin We use Gills III for histo and Gills I for cyto. BR -----Original Message----- From: mark.lewis@thermo.com [mailto:mark.lewis@thermo.com] Sent: Thursday, January 29, 2004 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin Hello everyone ! I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs. Thanks ! Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KathyOconnor <@t> hmh.westsound.net Thu Jan 29 12:20:12 2004 From: KathyOconnor <@t> hmh.westsound.net (Kathy OConnor) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Competency checklists Message-ID: Does anyone have a competency checklist for Histotechs that perform routine histology but no grossing or IHC they would like to share? Kathy O'Connor PAKC/DSL Bremerton, WA From gudrun.lang <@t> aon.at Thu Jan 29 12:27:06 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin References: Message-ID: <002501c3e695$7fe07d40$eeeea8c0@SERVER> We use Mayer's hematoxylin for routine HE, and Harris hematoxylin for cryocuts. Weigert is used with specistains. Gudrun Lang general hospital, Linz, Austria ----- Original Message ----- From: To: Sent: Thursday, January 29, 2004 4:31 PM Subject: [Histonet] Hematoxylin > Hello everyone ! > > I'm taking a survey to find out which type of Hematoxylin is most commonly > used in Histology and Cytology labs. > > Thanks ! > > Best regards, > > Mark > > Mark Lewis > Product Specialist > Anatomical Pathology > Clinical Diagnostics > Thermo Electron Corporation > (412) 747-4013 > (412) 788-1097 > E-mail: mark.lewis@thermo.com > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DDittus787 <@t> aol.com Thu Jan 29 12:41:35 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] looking for antibodies :HPL,EBV,AE1 AE3, BER-e P04 Message-ID: <75B93967.23A1D7FC.0A1F969F@aol.com> Josef: I too have a ventana system and have found that both the zymed concentrates and v-line work very well on the system. their number is 1-800-874-4494. if you need any protocols for these please feel free to contact me. Dana From info <@t> instrumedics.com Thu Jan 29 12:32:33 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] cartilage wrinkles Message-ID: <003001c3e696$43821fb0$6501a8c0@INSTRUMEDICS22> Maria, You might find the Paraffin Tape-Transfer system an answer to your problems. The section is captured on a tape window as it is cut and omitting the water bath the section is adhered to an adhesive-coated slide. The section is flat and unwrinkled. The adhesive is polymerized in 30 sec. under a Curing Lamp. the tape is peeled away in an inert solvent and the slide is ready to be deparaffinized Please visit www.instrumedics.com for all the details. We welcome your questions. Bernice schiller@instrumedics.com From RSRICHMOND <@t> aol.com Thu Jan 29 13:20:15 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Re: Hematoxylin Message-ID: <15c.2bdfe23a.2d4ab6ef@aol.com> Mark Lewis, Product Specialist, Anatomical Pathology, Clinical Diagnostics, Thermo Electron Corporation asks us: >>I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs.<< And Gary Gill responds: >>I recommend Gill's for everything.? ;-) The Gill hematoxylins are iodate-oxidized (Mayer) hematoxylins. I believe - please comment on this, Gary - that the term "Gill hematoxylin" is proprietary and cannot be used without Gary Gill's permission. The term "Harris hematoxylin" is correctly applied only to hematoxylins oxidized with mercuric oxide. Since mercury is a hazmat that (usually) is not permitted in present-day histology labs, the term "Harris hematoxylin" has come to mean any alum hematoxylin that the Marketing Department decides to call Harris hematoxylin. This usage is deplorable. Iodate-oxidized hematoxylins should not be called Harris hematoxylin. Bob Richmond still the Samurai Pathologist Gastonia NC From jcline <@t> wchsys.org Thu Jan 29 13:20:41 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] stains used Message-ID: <003201c3e69c$fc5632a0$1d2a14ac@wchsys.org> I use Gill's III and Eosin Y alcoholic From caroline.stott <@t> anatomy.otago.ac.nz Thu Jan 29 13:41:49 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: <5.2.1.1.0.20040130084033.024fcd40@anatomy.otago.ac.nz> We routinely use Harris Haematoxylin, but have most others at hand for when required. Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From garygill <@t> dcla.com Thu Jan 29 14:02:51 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Re: Hematoxylin Message-ID: Hello Bob: Gill hematoxylins more closely resemble Mayer's than Harris's hematoxylins in terms of hematoxylin concentration (2 gm/L [gill] vs. 1 gm/L [Mayer {5 gm/L for Harris}]) and iodate oxidizer (Harris uses mercuric oxide). However, Gill's No. 1: * Uses half the sodium iodate to oxidize the hematoxylin (0.1 gm/gm hematoxylin vs. Mayer's 0.2 gm) to compensate for the variable amount of hematein ordinarily present in hematoxylin (oxidized hematoxylin becomes hematein, which combines with the Al mordant to form a "lake" that colors chromatin [hematoxylin alone does not impart color]), and therefore, avoid overoxidation. Overoxidation produces oxyhematein, which is brownish, and shortens the useful life of a hematoxylin formulation. * Uses glacial acetic acid, rather than citric acid. Glacial acetic acid separates some of the aluminum ions from the hematein molecules to bring the working concentration of Al-hematein to approximately the same starting concentration each time a batch is prepared. * Uses 1 part ethylene glycol (antifreeze) to 3 parts water to stabilize the solution and to increase the solubility of the solution for Al-hematein. In Harris formulations, a greenish-golden surface precipitate forms on standing. This precipitate is sometimes identified as an overoxidation product. In fact, it is the "good stuff" -- pure Al-hematein. It forms because the ingredient hematoxylin continues to oxidize over time as the surface of the solution is exposed to atmospheric oxygen. Eventually, the concentration of Al-hematein thus formed exceeds its solubility limit in water, and so it precipitates. It won't precipitate, however, in a 25% ethylene glycol solution. The greenish-golden surface precipitate can be retrieved by filtering the Harris hematoxylin solution, and redissolved in 25% ethylene glycol. The resulting solution resembles a diluted hematoxylin solution. It stains a test buccal smear just as though it were full strength hematoxylin. Ergo, the precipitate is not junk, but the "good stuff". The late Irwin Lerner registered my surname with the US Patent and Trademark Office in 1973 without my permission. That trademark has expired, so anyone can use the Gill name with my formulation. My permission was not, and is not, required. In the mid-70s, Lerner was sued to block some companies (e.g., Polysciences) from using Gill in conjunction with my formulation. I assisted defense attorneys in fighting the suits. BTW, I receive no royalties for this product. However, I'm always open to potential offers. Gary Gill -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: Thursday, January 29, 2004 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Hematoxylin Mark Lewis, Product Specialist, Anatomical Pathology, Clinical Diagnostics, Thermo Electron Corporation asks us: >>I'm taking a survey to find out which type of Hematoxylin is most >>commonly used in Histology and Cytology labs.<< And Gary Gill responds: >>I recommend Gill's for everything.? ;-) The Gill hematoxylins are iodate-oxidized (Mayer) hematoxylins. I believe - please comment on this, Gary - that the term "Gill hematoxylin" is proprietary and cannot be used without Gary Gill's permission. The term "Harris hematoxylin" is correctly applied only to hematoxylins oxidized with mercuric oxide. Since mercury is a hazmat that (usually) is not permitted in present-day histology labs, the term "Harris hematoxylin" has come to mean any alum hematoxylin that the Marketing Department decides to call Harris hematoxylin. This usage is deplorable. Iodate-oxidized hematoxylins should not be called Harris hematoxylin. Bob Richmond still the Samurai Pathologist Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renpillai <@t> yahoo.com Thu Jan 29 14:22:34 2004 From: renpillai <@t> yahoo.com (Ren Pillai) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Alizarin red/alcian blues staining in zebrafish Message-ID: <20040129202234.12522.qmail@web60801.mail.yahoo.com> Hi, I am trying to find a protocol to stain some zebrafish 8days old embryos with alizarin red/alcian blue (double staining). Any help will be greatly appreciated. Thanks, Ren Renjitha Pillai Graduate Student Department of Biology University of Ottawa Ottawa Canada tel-613-562-5800 * 6329 --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! From juan.gutierrez <@t> christushealth.org Thu Jan 29 13:59:11 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] looking for antibodies :HPL,EBV,AE1 AE3, BER-e P04 Message-ID: Hello Josie, we use Ventana's lyop. EBV cat.#250-2758 with excellent results. For AE1/AE3 we use keratin(Pan) from Ventana cat.#760-2595 or if you want them separate we also use their products. We tried using concentrates, but we've got better and more consistent results with their predilutes. The HPL I run on my DAKO stainer for now, but I'm trying to move all my immunos to the benchmarks. Not enough time in the day for that, maybe I should get another Benchmark. The BER-ePO4 we do not have yet. I'm sure it will be added to our ever increasig inventory(122 and counting). It might not be much, but for a lab that only has 7,000 surgical specimens a year. it's a lot. Good luck with your search and sorry about rambling. Take care, Juan -----Original Message----- From: Nava, Josefa [mailto:JosefaNava@texashealth.org] Sent: Wed 1/28/2004 1:34 PM To: 'Histonet@pathology.swmed.edu' Cc: Subject: [Histonet] looking for antibodies :HPL,EBV,AE1 AE3, BER-e P04 Hello to Everyone! I am looking for antibodies that will work well with our Ventana Benchmark . The antibodies are HPL, AE1 AE3, EBV and BER-eP04. Thank you. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caroline.stott <@t> anatomy.otago.ac.nz Thu Jan 29 14:43:38 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: <5.2.1.1.0.20040130093901.024da590@anatomy.otago.ac.nz> Hi Mark, We use 5g Haematoxylin 100g Ammonium Alum 50ml Ethyl alcohol 1000ml Distilled water 0.37g Sodium iodate and when cool we add 40ml glacial acetic acid. So it is acidified. We use weak acid alcohol to differentiate the slides, followed by an alcoholic eosin Y. Good luck with the survey Caroline Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From JCarpenter764 <@t> aol.com Thu Jan 29 16:02:31 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: <70.3780b62d.2d4adcf7@aol.com> Our lab uses Gills Hematoxylin as well. Jennell From naje1972 <@t> yahoo.com Thu Jan 29 16:47:25 2004 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Staining protocols for cell cultures Message-ID: <20040129224725.43655.qmail@web41710.mail.yahoo.com> Hello everyone in Histoland. I was wondering if someone could share with me their protocols for H&E staining of cell cultures grown in wells. Is cold formalin ok to use for these cultures? Thanks in advance. Cynthia Haynes H.T. __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! http://webhosting.yahoo.com/ps/sb/ From AnthonyH <@t> chw.edu.au Thu Jan 29 17:01:02 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Re: Hematoxylin Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E11E@simba.kids> Bob, Good point re the naming of iodate oxidised Harris Haematoxylins. Any idea for a more appropriate name? How about "HENWOOD'S Haematoxylin" ????? (Hee Hee !!!!) Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: Friday, 30 January 2004 6:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Hematoxylin Mark Lewis, Product Specialist, Anatomical Pathology, Clinical Diagnostics, Thermo Electron Corporation asks us: >>I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs.<< And Gary Gill responds: >>I recommend Gill's for everything.? ;-) The Gill hematoxylins are iodate-oxidized (Mayer) hematoxylins. I believe - please comment on this, Gary - that the term "Gill hematoxylin" is proprietary and cannot be used without Gary Gill's permission. The term "Harris hematoxylin" is correctly applied only to hematoxylins oxidized with mercuric oxide. Since mercury is a hazmat that (usually) is not permitted in present-day histology labs, the term "Harris hematoxylin" has come to mean any alum hematoxylin that the Marketing Department decides to call Harris hematoxylin. This usage is deplorable. Iodate-oxidized hematoxylins should not be called Harris hematoxylin. Bob Richmond still the Samurai Pathologist Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From laurie.reilly <@t> jcu.edu.au Thu Jan 29 17:49:47 2004 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin In-Reply-To: Message-ID: <5.1.0.14.0.20040130094714.00a33580@mail.jcu.edu.au> We use Mayer's routinely, and add a Celestin Blue step for trichromes. Regards, Laurie. At 10:31 AM 01/29/04 -0500, mark.lewis@thermo.com wrote: >Hello everyone ! > >I'm taking a survey to find out which type of Hematoxylin is most commonly >used in Histology and Cytology labs. > >Thanks ! > >Best regards, > >Mark > >Mark Lewis >Product Specialist >Anatomical Pathology >Clinical Diagnostics >Thermo Electron Corporation >(412) 747-4013 >(412) 788-1097 >E-mail: mark.lewis@thermo.com > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly Ph 07 4781 4468 Physiology & Pharmacology Fax 07 4779 1526 Aust.Inst.of Tropical Vet.& Animal Sc. James Cook University Townsville Qld. 4811 laurie.reilly@jcu.edu.au Australia. From Loralee_Gehan <@t> URMC.Rochester.edu Wed Jan 28 09:10:34 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Articular cartilage wrinkles Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804CA@exmc3.urmc.rochester.edu> We cut our samples at 3microns. This seems to help. And we have also started using a slide warmer. Set at about 37C. Lay the slides on that after sectioning and let sit for a few hours or all day. Depending on your preference. This slide warmer has improved the way the sections stick to the slides and also eliminated the wrinkles. Our slide warmer came from Fisher Scientific. Around $500, but well worth it. We bought two. Hope that helps you. Loralee Gehan Orthopaedics Research lab > ---------- > From: Wadsworth, Marilyn P > Sent: Wednesday, January 28, 2004 9:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Articular cartilage wrinkles > > > Dear Histonet, > > > I am processing specimens of articular cartilage/bone from rabbit > > specimens. The tissue has been fixed and decaled using EDTA in > > formalin using the method of Kirviranta, 1985. > > > > Kiviranta, I., et al., Microspectrophotometric quantitation of > > glycosaminoglycans in articular cartilage sections stained with > > Safranin O. Histochemistry., 1985. 82(3): p. 249-55. > > > > The sections are paraffin embedded and sectioned at 5 or 6 microns. > > Upon mounting on slides, the articular cartilage appears puckered or > > wrinkled (result of swelling of cartilage when exposed to water). Is > > there anything that may be done to eliminate this artifact? Is it > > possible to float the microtomed sections on a non-aqueous solution? > > I'd appreciate any tips you may offer. Thanks > > > Maria Roemhildt, PhD > University of Vermont > Department of Orthopaedics & Rehabilitation > 95 Carrigan Drive > Burlington, VT 05405 > > Ph. 802-656-3823 > fax: 802-656-4247 > > maria.roemhildt@med.uvm.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kdink <@t> ruraltel.net Thu Jan 29 19:18:11 2004 From: kdink <@t> ruraltel.net (Kris Dinkle) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: <4019B0D3.8090905@ruraltel.net> We currently use Harris, Weigert and Gill Kris Histology Cytology Prep Central Plains Lab Hays,KS From drmoses <@t> erols.com Thu Jan 29 19:06:24 2004 From: drmoses <@t> erols.com (david moses) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] DAKO synaptophysin on Ventana ES Message-ID: <002d01c3e6cd$48fa6740$d4f63ad0@turbo> Is anyone using DAKO's synaptophysin monoclonal (sy38) on their Ventana ES or NexES? I used citrate buffer in a decloaker at a 1:20 titer, with no staining at all in a pancreas control. Renee Boston-Moses HT Virtua Health Ststems Voorhees,New Jersey From Loralee_Gehan <@t> URMC.Rochester.edu Wed Jan 28 09:10:34 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Articular cartilage wrinkles Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804CA@exmc3.urmc.rochester.edu> We cut our samples at 3microns. This seems to help. And we have also started using a slide warmer. Set at about 37C. Lay the slides on that after sectioning and let sit for a few hours or all day. Depending on your preference. This slide warmer has improved the way the sections stick to the slides and also eliminated the wrinkles. Our slide warmer came from Fisher Scientific. Around $500, but well worth it. We bought two. Hope that helps you. Loralee Gehan Orthopaedics Research lab > ---------- > From: Wadsworth, Marilyn P > Sent: Wednesday, January 28, 2004 9:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Articular cartilage wrinkles > > > Dear Histonet, > > > I am processing specimens of articular cartilage/bone from rabbit > > specimens. The tissue has been fixed and decaled using EDTA in > > formalin using the method of Kirviranta, 1985. > > > > Kiviranta, I., et al., Microspectrophotometric quantitation of > > glycosaminoglycans in articular cartilage sections stained with > > Safranin O. Histochemistry., 1985. 82(3): p. 249-55. > > > > The sections are paraffin embedded and sectioned at 5 or 6 microns. > > Upon mounting on slides, the articular cartilage appears puckered or > > wrinkled (result of swelling of cartilage when exposed to water). Is > > there anything that may be done to eliminate this artifact? Is it > > possible to float the microtomed sections on a non-aqueous solution? > > I'd appreciate any tips you may offer. Thanks > > > Maria Roemhildt, PhD > University of Vermont > Department of Orthopaedics & Rehabilitation > 95 Carrigan Drive > Burlington, VT 05405 > > Ph. 802-656-3823 > fax: 802-656-4247 > > maria.roemhildt@med.uvm.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From DDDeltour <@t> sig.med.navy.mil Thu Jan 29 23:55:47 2004 From: DDDeltour <@t> sig.med.navy.mil (Deltour, Douglas D.(HM2)) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Need your help Message-ID: First of all I wouldn't touch it with a ten foot pole. Secondly, do you have any training that would allow you to second guess a surgeon? I would toss it back in the Pathologist lap. If the surgeons find out that you are up to this ..... Your pathologist needs to take his concerns to the Board or Head of surgery. Run away from the light... HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy Anatomic Pathology, Histology Supervisor (HT) FROM US: 01139095564862 DSN: 624-4862 FAX FROM US: 01139095564680 DSN: 624-4680 DISCLAIMER: Confidentiality Notice - This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. **** Informazione Confidenziale - Questa e-Mail, incluso eventuali allegati, contiene informazioni confidenziali intese unicamente alla persona/e a cui e' indirizzata. Se avete ricevuto per errore questo messaggio, cortesemente contattare immediatamente il mittente e cancellare la e-Mail. Grazie****. -----Original Message----- From: Nita Searcy [mailto:NSEARCY@swmail.sw.org] Sent: Thursday, January 29, 2004 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need your help Have been asked to research surgeries that are happening frequently that pathologist believe that "are not standard-of-care" and could possibly be a research project or not necessary to procedure. Where would I go for help with the matter? Can-o-worms. A pathologist asked me to research??? Many thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This document may contain information covered under the privacy Act, 5 USC 552(a), and/or the Health Insurance Portability and Accountability Act (PL 104-191) and its various implementing regulations and must be protected in accordance with those provisions. Healthcare information is personal and sensitive and must be treated accordingly. If this correspondence contains healthcare information it is being provided to you after applying the appropriate security controls and authorization from the patient, or under circumstances that don't require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Redisclosure without additional patient consent or as permitted by law is prohibited. Unauthorized redisclosure or failure to maintain confidentiality subjects you to application of appropriate sanction. If you have received this correspondence in error, please notify the sender and the command Privacy Officer at privacy@sig.med.navy.mil at once and destroy any copies you have made. From nick.kirk3 <@t> btopenworld.com Fri Jan 30 01:35:07 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Haematoxylin Message-ID: We use Gill's 3 for our routine H&Es and immuno counterstain, Celestin Blue/Carazzi's Hx for some of our Special stains and Harris's for our Pap stains and Cytology H&Es. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England From gudrun.lang <@t> aon.at Fri Jan 30 05:19:39 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin References: <000601c3e6bf$3a5e5ab0$014dbad0@hppav> Message-ID: <004b01c3e722$f372fed0$eeeea8c0@SERVER> George, thanks for your advice. Yes, we belong to this old fashioned labs, that mix their stain solutions by themeselves. We tried the purchaseable hematoxilines, but our pathologiest did not like any change. Because of the steady exchange of solutions, there is a constant colouring. The hematoxilin bottles are used up within a month. It is a kind of question of believe, I think. greetings Gudrun ----- Original Message ----- From: "George Cole" To: "'Gudrun Lang'" Sent: Friday, January 30, 2004 12:25 AM Subject: RE: [Histonet] Hematoxylin > Gudrun > If you can find a source, GILL's III would replace all three of your > hematoxylins. It keeps so well, you might well be able to order enough > from Sigma or some other supplier to last for months. Mayer's, remember, > lasts only 30 days after being mixed. It does not stain steadily for 30 > days then die---it starts fading day by day from the beginning, thus > bringing in some variation you don't want. Harris demands clarification > with acid alcohol, thus letting in variations between those who do the > dunking in the acid alcohol, and Weigert's---I will make no comment on > Weigert's---I never used it---I simply saw the Weigert mixtures dating > from the 1900's. If it compares favorably with the Gill's III I will > swim from here to Linz without my water wings!!! > georgecole@ev1.net > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Thursday, January 29, 2004 10:27 AM > To: Histonetliste; mark.lewis@thermo.com > Subject: Re: [Histonet] Hematoxylin > > We use Mayer's hematoxylin for routine HE, and Harris hematoxylin for > cryocuts. > Weigert is used with specistains. > > Gudrun Lang > general hospital, Linz, Austria > > ----- Original Message ----- > From: > To: > Sent: Thursday, January 29, 2004 4:31 PM > Subject: [Histonet] Hematoxylin > > > > Hello everyone ! > > > > I'm taking a survey to find out which type of Hematoxylin is most > commonly > > used in Histology and Cytology labs. > > > > Thanks ! > > > > Best regards, > > > > Mark > > > > Mark Lewis > > Product Specialist > > Anatomical Pathology > > Clinical Diagnostics > > Thermo Electron Corporation > > (412) 747-4013 > > (412) 788-1097 > > E-mail: mark.lewis@thermo.com > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From CCLYATT <@t> mail.mcg.edu Fri Jan 30 05:34:45 2004 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Competency checklists Message-ID: I'm also interested to know how others are handling this. I've written a test with items I find techs most often forget that come from the SOP manual as well as technical questions directed to thier level of responsibility. I'm asking each tech to complete before their annual review. I'm waiting for the first test to be returned now. I would also like to develop a checklist of skills to be observed on a random basis. Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Kathy OConnor" 01/29/04 01:20PM >>> Does anyone have a competency checklist for Histotechs that perform routine histology but no grossing or IHC they would like to share? Kathy O'Connor PAKC/DSL Bremerton, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Jan 30 06:50:40 2004 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Competency checklists Message-ID: <90092A4ED388D7119575006008F7112049CB61@NT_EXCHANGE> I use a checklist for the routine things. It covers the basics such as cutting, maintenance, processing, staining, etc. It does have a section on Cytology and IHC so that I can use the same one for everybody. I would be happy to share it. It may not suit your individual needs but you can use it as a starting point. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio mailto:tmcnemar@lmhealth.org -----Original Message----- From: Claye Clyatt [mailto:CCLYATT@mail.mcg.edu] Sent: Friday, January 30, 2004 6:35 AM To: KathyOconnor@hmh.westsound.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Competency checklists I'm also interested to know how others are handling this. I've written a test with items I find techs most often forget that come from the SOP manual as well as technical questions directed to thier level of responsibility. I'm asking each tech to complete before their annual review. I'm waiting for the first test to be returned now. I would also like to develop a checklist of skills to be observed on a random basis. Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Kathy OConnor" 01/29/04 01:20PM >>> Does anyone have a competency checklist for Histotechs that perform routine histology but no grossing or IHC they would like to share? Kathy O'Connor PAKC/DSL Bremerton, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VAbbott <@t> Trianglebiomedical.com Fri Jan 30 08:02:08 2004 From: VAbbott <@t> Trianglebiomedical.com (Valerie Abbott) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Mounting Media for Hacker Coverslippers Message-ID: <600E7CD630C81542ADCA1457EB6A8F4D04D810@tbs01.trianglebiomed.com> TBS is the exclusive North American Importer of the SHUR/Mount RCM7000 Glass Coverslipper. The instrument is produced in Japan by Meisei, the supplier of the now discontinued coverslipper models 3655 and 3660, previously marketed by Hacker. SHUR/Mount utilizes a xylene bath to stage slides, a xylene reservoir to prevent the dispensing needle from drying out as well as xylene to lubricate the dispensing system pump. Toluene based mounting media should not be utilized as the functionality of the instruments is matched to the evaporation rate of xylene. Toluene was also not chosen because of its more hazardous properties to human health Various viscosities of xylene based mounting media can be used in SHUR/Mount and are available by multiple suppliers. TBS offers a mounting medium that is formulated for use with the RCM7000 and is also compatible with the 3555 and 3660. The viscosity is specifically formulated to match the midpoint of the pressure ranges of the pumping system of the instrument. Jack Hunnell http://www.trianglebiomedical.com/ From BoozerKA <@t> pa1.ah.org Fri Jan 30 08:36:30 2004 From: BoozerKA <@t> pa1.ah.org (Kathleen Boozer) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Re: Hematoxylin Message-ID: We use Thermo Shandon Instant Hematox # 6765015 as a regressive manual stain....small hospital. >>> 01/29/04 11:20AM >>> Mark Lewis, Product Specialist, Anatomical Pathology, Clinical Diagnostics, Thermo Electron Corporation asks us: >>I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs.<< And Gary Gill responds: >>I recommend Gill's for everything. ;-) The Gill hematoxylins are iodate-oxidized (Mayer) hematoxylins. I believe - please comment on this, Gary - that the term "Gill hematoxylin" is proprietary and cannot be used without Gary Gill's permission. The term "Harris hematoxylin" is correctly applied only to hematoxylins oxidized with mercuric oxide. Since mercury is a hazmat that (usually) is not permitted in present-day histology labs, the term "Harris hematoxylin" has come to mean any alum hematoxylin that the Marketing Department decides to call Harris hematoxylin. This usage is deplorable. Iodate-oxidized hematoxylins should not be called Harris hematoxylin. Bob Richmond still the Samurai Pathologist Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BoozerKA <@t> pa1.ah.org Fri Jan 30 08:38:38 2004 From: BoozerKA <@t> pa1.ah.org (Kathleen Boozer) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Competency checklists Message-ID: NSH has a good one. >>> "Claye Clyatt" 01/30/04 03:34AM >>> I'm also interested to know how others are handling this. I've written a test with items I find techs most often forget that come from the SOP manual as well as technical questions directed to thier level of responsibility. I'm asking each tech to complete before their annual review. I'm waiting for the first test to be returned now. I would also like to develop a checklist of skills to be observed on a random basis. Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Kathy OConnor" 01/29/04 01:20PM >>> Does anyone have a competency checklist for Histotechs that perform routine histology but no grossing or IHC they would like to share? Kathy O'Connor PAKC/DSL Bremerton, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Fri Jan 30 09:01:39 2004 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin Message-ID: Richard-Allan makes a beautiful stain...I've used it in almost everywhere I've worked in the past years. You pay a little more but for automatic stainers the combination of Hematoxylin, bluing, and clarifier and Eosin (all RA products) it gives you a consistent stain. Our doctors really like it and so have the others. I also have competency check lists for those of you who need it. I have a check list (observation) in some areas of our work... ex. embedding, cutting, and equipment operation. I have a checklist for special stains of the main stains we perform and observation for technique. (We check the slides under the scope plus grade on technique). Also I have a separate checklist for computer functions and immuno and a test for immuno. I created it in Excel ....I will be glad to email so you can adjust for your institution. Carole Fields Lex Med Ctn W.Columbia, SC _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From DDittus787 <@t> aol.com Fri Jan 30 09:09:22 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] DAKO synaptophysin on Ventana ES Message-ID: <281D156B.2C0BAA79.0A1F969F@aol.com> renee: try 1:80 with protease 1 for 4 min and incubate for 32 min, our islet cells light up nice, by the way make sure you use dako diluent, not ventanas. Dana From cgfields <@t> lexhealth.org Fri Jan 30 09:11:52 2004 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Competency Forms Message-ID: In case this was not seen because of the Hematoxylin remark. I also have competency check lists for those of you who need it. I have a check list (observation) in some areas of our work... ex. embedding, cutting, and equipment operation. I have a checklist for special stains of the main stains we perform and observation for technique. (We check the slides under the scope plus grade on technique). Also I have a separate checklist for computer functions and immuno and a test for immuno. I created it in Excel ....I will be glad to email so you can adjust for your institution. Carole Fields Lex Med Ctn W.Columbia, SC _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From AJohnson <@t> system1.net Fri Jan 30 09:32:01 2004 From: AJohnson <@t> system1.net (Amy Johnson) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Histology Positions in New Jersey Message-ID: Good Morning! I am currently working with a rapidly growing client in NJ that has needs for both a supervisor and techs. These positions require strong IHC skills and the ability to thrive in a fast-paced environment. They are offering competitive salaries and excellent benefits. Techs need to have at least 2 years experience doing both routine histology and IHC. They heavily prefer HT, HTL, QIHC. Supervisors need at least 5 years plus supervisory experience. Minimum of HT, but HTL/QIHC is preferred. For further information or to be considered, please contact Amy Johnson at toll free: 866-797-8361 or via email ajohnson@system1.net Amy Johnson System 1 Search (864) 627-0012 (864) 627-0013 Fax From mcauliff <@t> umdnj.edu Fri Jan 30 10:12:20 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Need your help In-Reply-To: References: Message-ID: <401A8264.40508@umdnj.edu> Dear Nita: Keeping in mind that I have NO clinical experience ........... I think Doug is correct. Sounds like someone has given the pathologist a job he does not want to do and he is dumping it on you and trying to avoid blame. Where are you supposed to find this data, the Journal of SubStandard Care? Also, might YOU get blamed for trying to second guess surgeons? If he wants you to pull blocks/slides/reports on some cases that HE selects, sounds OK to me (again keeping in mind that I am not involved in clinical matters). Good luck! Geoff Deltour, Douglas D.(HM2) wrote: >First of all I wouldn't touch it with a ten foot pole. Secondly, do you have >any training that would allow you to second guess a surgeon? I would toss it >back in the Pathologist lap. If the surgeons find out that you are up to >this ..... Your pathologist needs to take his concerns to the Board or Head >of surgery. Run away from the light... > >HM2(FMF) Douglas D. Deltour >Naval Hospital Sigonella Italy >Anatomic Pathology, Histology Supervisor (HT) >FROM US: 01139095564862 DSN: 624-4862 >FAX FROM US: 01139095564680 DSN: 624-4680 > > >DISCLAIMER: Confidentiality Notice - This e-mail message, including any >attachments, is for the sole use of the intended recipient(s) and may >contain confidential and privileged information. Any unauthorized review, >use, disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all copies >of the original message. **** Informazione Confidenziale - Questa e-Mail, >incluso eventuali allegati, contiene informazioni confidenziali intese >unicamente alla persona/e a cui e' indirizzata. Se avete ricevuto per errore >questo messaggio, cortesemente contattare immediatamente il mittente e >cancellare la e-Mail. Grazie****. > > >-----Original Message----- >From: Nita Searcy [mailto:NSEARCY@swmail.sw.org] >Sent: Thursday, January 29, 2004 6:37 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Need your help > > >Have been asked to research surgeries that are happening frequently that >pathologist believe that "are not standard-of-care" and could possibly >be a research project or not necessary to procedure. Where would I go >for help with the matter? > >Can-o-worms. > >A pathologist asked me to research??? > >Many thanks > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >This document may contain information covered under the privacy Act, 5 USC >552(a), and/or the Health Insurance Portability and Accountability Act (PL >104-191) and its various implementing regulations and must be protected in >accordance with those provisions. Healthcare information is personal and >sensitive and must be treated accordingly. If this correspondence contains >healthcare information it is being provided to you after applying the >appropriate security controls and authorization from the patient, or under >circumstances that don't require patient authorization. You, the recipient, >are obligated to maintain it in a safe, secure and confidential manner. >Redisclosure without additional patient consent or as permitted by law is >prohibited. Unauthorized redisclosure or failure to maintain confidentiality >subjects you to application of appropriate sanction. If you have received >this correspondence in error, please notify the sender and the command >Privacy Officer at privacy@sig.med.navy.mil at once and destroy any copies >you have made. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From JCarpenter764 <@t> aol.com Fri Jan 30 10:17:07 2004 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] correct answer in book.... Message-ID: <0FF889D1.408E511E.40C6E687@aol.com> Hey maybe you guys can be of some help...I recently bought the second edition board of registry study guide. when i received this book a little note came with it telling me that one of the answers to a question was misprinted. The note also gave me the correct answer. I can't remember what section it was or the page number. I have lost the little piece of paper.Maybe someone can help....Thanks Jennell From ttroyer <@t> pcllab.com Fri Jan 30 10:21:18 2004 From: ttroyer <@t> pcllab.com (Travis Troyer) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] OUTSIDE CPT CODE AUDIT Message-ID: <001801c3e74d$17a12280$6601010a@Peterson.local> I was curious to know if any other labs have had an outside company come in and audit the billing procedure to make sure all possible techniques are being billed adequately. I was wanting to find out opinions on the pros and cons of having this done and if it was advantageous. Thanks, Travis From dencrowl <@t> MIT.EDU Fri Jan 30 10:19:49 2004 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] employment at MIT in Cambridge Ma Message-ID: We are looking to add a histotech to our Histology Core Facility in the Center for Cancer Research at Massachusetts Institute of Technology. Our staff consists of 2 techs and a facility manager working for 20 different laboratories all interested in researching the genetic basis for cancer. Our work consists of processing, sectioning, and H&E staining of animal tissues (mostly mouse, but some zebrafish as well). We have the latest in automated equipment including a ThermoElectron Excelsior processor, ThermoElectron Finesse microtomes, ThermoElectron Cryotome, ThermoElectron Gemini Stainer, and ThermoElectron Consul Coverslipper We are looking for a full-time tech with Histology experience. While certification is not required, we need someone who is committed to staying in Histology and who can serve as a resource for grad students and postdoctoral fellows in helping to design their experiments. Hours are flexible and benefits are great. If you are interested in a challenging environment where your expertise is truly appreciated, send me a resume and we will talk. Denise Crowley, Facility Manager Histology Massachusetts Institute of Technology Center for Cancer Research 40 Ames St. E17-230 Cambridge MA 02139 dencrowl@mit.edu From JNocito <@t> Pathreflab.com Fri Jan 30 10:33:38 2004 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Need your help In-Reply-To: <04Jan29.113726cst.119126@healthcare2.sw.org> Message-ID: Run, don't walk away from this. I may second guess a pathologist when they order off the wall immunos, but we don't the education, training or experience to second guess a surgeon. That's my story and I'm sticking to it. Joe Nocito, BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nita Searcy Sent: Thursday, January 29, 2004 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need your help Have been asked to research surgeries that are happening frequently that pathologist believe that "are not standard-of-care" and could possibly be a research project or not necessary to procedure. Where would I go for help with the matter? Can-o-worms. A pathologist asked me to research??? Many thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Jan 30 10:40:28 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Need your help Message-ID: Joe writes: "Run, don't walk away from this. I may second guess a pathologist when they order off the wall immunos, but we don't (have) the education, training or experience to second guess a surgeon. That's my story and I'm sticking to it." Absolutely, and in my view, neither do pathologists (though we may on occasions have our suspicions). Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Joe Nocito [mailto:JNocito@Pathreflab.com] Sent: 30 January 2004 16:34 To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need your help Joe Nocito, BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Nita Searcy Sent: Thursday, January 29, 2004 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need your help Have been asked to research surgeries that are happening frequently that pathologist believe that "are not standard-of-care" and could possibly be a research project or not necessary to procedure. Where would I go for help with the matter? Can-o-worms. A pathologist asked me to research??? Many thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Fri Jan 30 11:47:07 2004 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] To All Tamtron/PowerPath users Message-ID: <401A989B.3080808@pathology.washington.edu> A new list server was created by my boss Dr. Rod Schmidt with support from the University of Washington. The PowerPath Mutual Assistance (PPMA) email list is a forum for system administrators, developers, superusers and users to disucss issues and solutions related to PowerPath. We hope it will be a positive forum and a way for us to share information that will help us all provide excellent patient care through Pathology informatics. General information about the mailing list is at: https://mailman.u.washington.edu/mailman/listinfo/ppma Please forward this to anyone who may be interested. Victor -- Victor Tobias Clinical Applications Analyst Dept of Pathology University of Washington Medical Center 206-543-4823 From cgfields <@t> lexhealth.org Fri Jan 30 11:49:52 2004 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Competency Message-ID: Glad I could help. Carole fields _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From jcline <@t> wchsys.org Fri Jan 30 13:18:18 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Validation for IHC Message-ID: <002001c3e765$d180d3e0$1d2a14ac@wchsys.org> Does anyone send their positive control tissue to another lab for validation of their immuno staining? The path that is over the immuno area wants me to varify the use of my patient controls by sending to another lab to show that these controls are positive by another source. Has anyone been CAP inspected and asked if they do this? Seems he thinks an inspector is going to ask this. From MAUGER <@t> email.chop.edu Fri Jan 30 13:27:22 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Validation for IHC Message-ID: To Joyce, No, I have never heard of sending controls to another lab for verification. This sounds unreasonable because other labs use different chemistrys,different dilutions,different fixation,etc. your pathologist should be able to verify for herself wether the control tissue is positive,how did they get diagnosed in the first place? Are they sent out to verify the diagnosis? This doesn't make sense to me. Jo >>> "Joyce Cline" 01/30/04 02:18PM >>> Does anyone send their positive control tissue to another lab for validation of their immuno staining? The path that is over the immuno area wants me to varify the use of my patient controls by sending to another lab to show that these controls are positive by another source. Has anyone been CAP inspected and asked if they do this? Seems he thinks an inspector is going to ask this. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Fri Jan 30 13:47:09 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Validation for IHC Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20357FA@usca0082k08.labvision.apogent.com> Joyce, That is one way to validate how well your system works. I used to do that with our controls. It is also helpful to reciprocate with the other lab. It is especially helpful if the other lab uses a different kit/chemistry than your lab uses. You can also do it by: 1) using different(but same target)antibodies and kits in house on the same tissue to control for kit variations, 2) using non-sense antibodies (those that should not stain on the tissue/tumor)to control for non-specific tissue staining, 3) checking specificity by testing non-target tissue with the antibody and 4) comparing several control blocks of varying staining intensity (tisse arrays are good for this type of test as well as #3). If you choose to use another lab I would suggest random testing - not everything. Doing everything that way can get mighty complicated and add a lot to the workload. Maybe even just your most heavily used antibodies. Tim Morken Lab Vision / Neomarkers www.labvision.com -----Original Message----- From: Joyce Cline [mailto:jcline@wchsys.org] Sent: Friday, January 30, 2004 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validation for IHC Does anyone send their positive control tissue to another lab for validation of their immuno staining? The path that is over the immuno area wants me to varify the use of my patient controls by sending to another lab to show that these controls are positive by another source. Has anyone been CAP inspected and asked if they do this? Seems he thinks an inspector is going to ask this. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Fri Jan 30 14:01:54 2004 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Validation for IHC Message-ID: I have been thru 2 CAP inspections and have never seen that question. The important aspect is that the positive control tissues stain appropriately in your laboratory. If one of my pathologists has a question about an IHC test, we send a few of the tests in question to be validated. I would think it would be the decision of the pathologist in charge and what he/she is comfortable with. Then there is the issue of billing, payment, etc., but that's another subject. Jacquie Poteete MT(ASCP) QIHC, Lead Technologist IHC Lab Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com > -----Original Message----- > From: Joyce Cline [SMTP:jcline@wchsys.org] > Sent: Friday, January 30, 2004 1:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Validation for IHC > > Does anyone send their positive control tissue to another lab for > validation of their immuno staining? > The path that is over the immuno area wants me to varify the use of > my patient controls by sending to another lab to show that these controls > are positive by another source. > Has anyone been CAP inspected and asked if they do this? Seems he > thinks an inspector is going to ask this. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From psrice <@t> email.arizona.edu Fri Jan 30 14:25:55 2004 From: psrice <@t> email.arizona.edu (Photini Faith S. Rice) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Automated microtome for plastic sectioning Message-ID: <1075494355.c0779096660d2@www.email.arizona.edu> Could anyone give an opinion on the most popular Automated microtomes for sectioning MMA and other plastics? From staceylburton <@t> yahoo.com Fri Jan 30 14:38:05 2004 From: staceylburton <@t> yahoo.com (Stacey Burton) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] pregnancy in histology lab Message-ID: <20040130203805.31447.qmail@web14521.mail.yahoo.com> I am performing a pole for some inquiring employees. If anyone would like to comment - Thank you. 1. Have you ever been pregnant while working with in the histology laboratory? 2. Did your work duties have to be altered while being pregnant and to what extent? 3. Did working in the histology laboratory effect your pregnancy in any negative way? Our laboratory does perform regular ppm testing for xylene and formalin. Thank you for any comments for our pole, Stacey L. Burton, H.T. ASCP Laboratory Manager Unipath LLC San Antonio TX 210) 521-7700 staceylburton@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! From pruegg <@t> colobio.com Fri Jan 30 14:55:04 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:30 2005 Subject: [Histonet] Hematoxylin In-Reply-To: Message-ID: I use Gills III for everything. I dilute it for IHC. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of BRobert@ameripath.com Sent: Thursday, January 29, 2004 10:29 AM To: mark.lewis@thermo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hematoxylin We use Gills III for histo and Gills I for cyto. BR -----Original Message----- From: mark.lewis@thermo.com [mailto:mark.lewis@thermo.com] Sent: Thursday, January 29, 2004 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin Hello everyone ! I'm taking a survey to find out which type of Hematoxylin is most commonly used in Histology and Cytology labs. Thanks ! Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Fri Jan 30 15:32:34 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Ideal endothelial cell-isolatin' antibody In-Reply-To: <9122C182D4268F45BCB9E64A10B7331F024D126F@1upmc-msx7.isdip.upmc.edu> Message-ID: Jim, Your best bet would be CD31, other alternatives would include cd34, factor VIII, and vegf, but cd31 is probably the most specific for just endothelial cells, the others stain a lot of other things as well. We didn't get endoglin to work very well in ffpe tissue, it was inconsistant. You should be able to biotinylate or buy a bt cd31 and do a quick sensitive detection for it. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gagermeier, James Sent: Tuesday, January 27, 2004 9:41 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ideal endothelial cell-isolatin' antibody I am attempting to find an antibody for immunofluorescence that is the best (from experience) in isolating endothelial cells. This is significant for me as I am attempting to isolate endothelail cells in order to perform laser microdissection on these cells for further studies. The problem I am faced with is that an approach utilizing double staining will take too long, allowing RNA degradation. It is reccomended that a specific primary biotinylated antibody be used - and then a rapid IF staining kit by Arcturus is used. The IF protocol appears to take 12-15 minutes. I am trying to determine the best antibody so that I can use a single biotinylated primary antibody with the rapid Arcturus IF protocol. Any reccs are appreciated. Thanks Jim Gagermeier _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KYAMAMOTO <@t> chromavision.com Fri Jan 30 16:03:15 2004 From: KYAMAMOTO <@t> chromavision.com (Yamamoto, Karen) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] positions available Message-ID: ChromaVision Medical Systems, the leader in cellular imaging diagnostics and manufacturer of the Automated Cellular Imaging System (ACIS*), is seeking self-starters with excellent communication, interpersonal, multi-tasking, and organizational skills for the following positions: Customer Service Coordinator/Dispatcher Provides centralized call coordination including tracking/documenting calls, and resolves client services issues. Qualifications: working knowledge of MS Word, excellent customer service skills and laboratory/oncology experience desired. High school or GED certificate plus a minimum of two years customer service experience required. Laboratory/Operations Assistant Provides general administrative support including receiving/entering patient data, monitoring specimens, responding to clients/trial managers and interacting with national/local couriers. Qualifications: MS Word, accurate data entry skills and ability to multi-task desired. High school or GED certificate plus two years medical administrative support in a laboratory/clinical trial or oncology work environment required. IHC Technician Performs image quality IHC stains, routine histology and participates in training. Requires the ability to handle tissue, blood/blood products, work aseptically, comprehend/follow written protocols and document accurate results. Qualifications: Knowledge/skill in tissue culture, immunohistochemistry, histology, assay development and IHC laboratory quality control. BS/MS in Biological Sciences or technical certification plus a minimum of two years clinical/commercial laboratory experience. Histo Technician Performs routine histology, image quality IHC stains, and participates in training. Requires the ability to handle tissue, blood/blood products, work aseptically, comprehend/follow written protocols and document accurate results. Qualifications: Knowledge/skill in tissue culture, immunohistochemistry, histology, assay development and IHC laboratory quality control. BS/MS in Biological Sciences or technical certification plus a minimum of two years clinical/commercial laboratory experience. We offer a competitive compensation and benefits package and enjoy a team-based approach to success in a business-casual work environment. For consideration, send resume indicating position of interest and salary requirements to: ChromaVision Medical Systems, 33171 Paseo Cerveza, San Juan Capistrano, CA 92675; Fax: (949) 443-5257; E-mail: dwilliams@chromavision.com EOE > > > > From gudrun.lang <@t> aon.at Fri Jan 30 16:27:20 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] pregnancy in histology lab References: <20040130203805.31447.qmail@web14521.mail.yahoo.com> Message-ID: <002c01c3e780$3a0f57a0$eeeea8c0@SERVER> My children are 8 and 11 years old, and until now they are obviosly healthy. During pregnancy I was not allowed to be in rooms with formalin, xylol or any other probably cancerogen reagens. I was not allowed to handle with nativ probes, like making cryo sections or grossing. My duties were cutting in a seperate room and doing administrative jobs. I had a coworker, who had her children in the 1960s. In this time, there were no fumehoods and xylol-wet slides were dried near the radiator. Using gloves was not really appriciated. But yet her girls are healthy and have also healthy children. But something irritated me, when she told about the good old times. Her elder colleages didn't reach a really high age and often had some cancer disease?! hope this helps Gudrun Lang Linz, Austria ----- Original Message ----- From: "Stacey Burton" To: Sent: Friday, January 30, 2004 9:38 PM Subject: [Histonet] pregnancy in histology lab > I am performing a pole for some inquiring employees. If anyone would like to comment - Thank you. > > 1. Have you ever been pregnant while working with in the histology laboratory? > > 2. Did your work duties have to be altered while being pregnant and to what extent? > > 3. Did working in the histology laboratory effect your pregnancy in any negative way? > > Our laboratory does perform regular ppm testing for xylene and formalin. > > Thank you for any comments for our pole, > > Stacey L. Burton, H.T. ASCP > Laboratory Manager > Unipath LLC > San Antonio TX > 210) 521-7700 > staceylburton@yahoo.com > > > > > > --------------------------------- > Do you Yahoo!? > Yahoo! SiteBuilder - Free web site building tool. Try it! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MTitford <@t> aol.com Fri Jan 30 16:28:45 2004 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: "Need your help" Message-ID: <2327C2DA.3B33AFF3.00762DB1@aol.com> Nita Searcy asks about investigating possible unneeded surgical procedures. Our hospital, as part of its JCAHO accreditation (Joint Council for the Accretation of Hospitals Organization or something close to that)has a "Surgical Case Review Committee". It meets monthly and reviews suspect or unnecessary surgery's among other things. It also takes on little projects like reviewing appendix operations to make sure they are needed. Also as part of our accretation processes, we require a surgical pathology slip be issued for each surgery that takes place, even if there is no specimen. The idea is the pathologist reviews the slip and if he/she thinks it is unnecessary,or there should have been a specimen, the slip is sent on to the "Surgical Case Review Committee". The scrub nurse signs these off as "none" where the specimen should be listed. (I served on the committe for a couple of years. It was quite interesting. We sat in plush chairs in the hospital boardroom and got fed lunch....) Mike Titford USA Pathology Mobile AL USA From kdink <@t> ruraltel.net Sat Jan 31 08:34:24 2004 From: kdink <@t> ruraltel.net (Kris Dinkle) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Pregnancy in the workplace Message-ID: <401BBCF0.2030808@ruraltel.net> Hello everyone. In regards to pregnancy in the Histology Lab, I have been through one pregnancy. I did not require any changes to my schedule or tasks. I had a very normal pregnancy and a healthy baby boy as expected. In addition to myself, a co-worker became pregnant with twins a year later. She also had a "normal" pregnancy, although, because of the amount of weight she gained (she was wider than she was tall), she eventually had difficulties in some tasks and did deliver a healthy boy and girl about a month early. Kris Dinkel Histology Hays, KS From stancelb <@t> msn.com Sat Jan 31 12:04:38 2004 From: stancelb <@t> msn.com (Barbara Stancel) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] pregnancy in histo, my story Message-ID: Dear Stacey, I began in histology in the very early 1970's. I was fresh out of college and technical school. Histology was a portion of my overall position as a grant tech for four Vet college professors and the department head. I had never heard of ASCP and almost nothing about safety. I had an unventilated room with one window. It was at the end of the hall and the end of the heating and AC ducts!! So, I had to run a fan in the summer and a little portable heater in the winter. Neither appliance was grounded or had any safety features. I did lab animal necropsies, fixation, processing(a brand new Technicon that lifted the tissues out of the solvents!), embedding, microtomy, staining, coverslipping in the same 16'X20' room. All the solvents were stored in there along with any acids, bases or any other chemicals I needed. I did about 250 to 350 blocks a month. And produced an average of 1500 slides a month because I did all the student sets for parasitology, along with the research and grad student work. Universities were not know for their desire to provide a safe environment for their employees. I also did all of the clinical parasitology for the college. Two days a week, I did ALL fecal parasite counts for the small and large animal clinics and all mail-in material, as well as total gut splits from necropsy. Total gut splits? Yep, you take the entire digestive tract of an animal (a 50 gallon trashcan full from an adult cow), split it and scrap all the contents out, separate the fecal matter from the intestinal parasites and count the parasites. Sounds awful, but it was very interesting. My position did not change during my pregnancy. My department head was mad at me for being pregnant!!! He was so upset that he quit talking to me in my 8th month until 3 months after I came back to work. He even tried to prevent me from using sick/maternity leave. He considered my "condition" was "elective" not medical. He always made sure that no one was available to help me. I can remember being so pregnant I had to turn sideways to get close enough to the big sinks (the kind that you could bath a St. Bernard in) to handle those gut splits. I am still grateful to all the Vet students and necropsy room techs I drafted from the hallways to help me get those 50 gallons of guts going. I was young and naive and not terribly assertive. When I think back I realize I was discriminated for being female and pregnant. But I had a good paying job and benefits. And since my husband was still in college, I could not quit. It was a marvelous learning experience for me. I learned ALOT about histology, parasites, and myself. I don't know why I still have a nose, lungs or brains. I had one uneventful pregnancy. A very healthy baby. Through the grace of God, my grown daughter is quite normal. I am not proud of my lack of knowledge during those times. I was not stupid. I just didn't know any better. It wasn't until the late 1970's that I started learning about all the hazards. By that time, I had switched to a new position in a federal laboratory where safety was developing as a new issue. My, My how things have changed!! Barbara Athens, Georgia From: Stacey Burton To: histonet@pathology.swmed.edu Subject: [Histonet] pregnancy in histology lab Date: Fri, 30 Jan 2004 12:38:05 -0800 (PST) I am performing a pole for some inquiring employees. If anyone would like to comment - Thank you. 1. Have you ever been pregnant while working with in the histology laboratory? 2. Did your work duties have to be altered while being pregnant and to what extent? 3. Did working in the histology laboratory effect your pregnancy in any negative way? Our laboratory does perform regular ppm testing for xylene and formalin. Thank you for any comments for our pole, Stacey L. Burton, H.T. ASCP Laboratory Manager Unipath LLC San Antonio TX 210) 521-7700 staceylburton@yahoo.com _________________________________________________________________ Get a FREE online virus check for your PC here, from McAfee. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From bmcmahill <@t> incytepathology.com Sat Jan 31 12:05:50 2004 From: bmcmahill <@t> incytepathology.com (Bonnie J. McMahill) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] pregnancy in histology lab Message-ID: <3F7537D9A2DE6D40BA884DF1A32A08CF16E694@PATH2K-SRV2.PAI.E-PATHOLOGY.COM> I've been working in the histology lab for almost 30 years (yikes!!) - so... many of my co-workers and myself have gone thru pregnancies. I can't think of any out of the normal pregnancies, deliveries, or health effects that could be attributed with our work environment. We currently try to accommodate for any restriction-requests by the pregnant worker. If they don't want any fume exposure, then they won't be assigned duties such as coverslipping (which is automated) or working in the gross room. It's always good to stress the use of PPE's to everyone, especially when a concern about pregnancy is brought up. Our fumes are carefully monitored, and not a risk for any of the staff, but I do understand the concerns when pregnant. Recently a new mom expressed concern about what she was doing during the 1st few weeks before she found out she was pregnant. Her main concern was the 3-4 drinks of alcohol she had on a Saturday night, not what she was doing at work.... Bonnie McMahill InCyte Pathology Spokane, WA -----Original Message----- From: Stacey Burton [mailto:staceylburton@yahoo.com] Sent: Fri 1/30/2004 12:38 PM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] pregnancy in histology lab From kildee6093 <@t> msn.com Sat Jan 31 12:31:18 2004 From: kildee6093 <@t> msn.com (MARILYN MCDONALD) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Fw: Histology Position Message-ID: ----- Original Message ----- From: Marilyn McDonald To: kildee6093@msn.com Sent: Friday, January 30, 2004 4:42 PM Subject: Fw: Histology Position ----- Original Message ----- From: Marilyn McDonald To: Histonet-requests@lists.utsouthwestern.edu Sent: Thursday, January 29, 2004 4:58 PM Subject: Histology Position January 29, 2004 GI Pathology Partners, PLLC is presently looking to fill a full time Histology position. Applicants desired are HT (ASCP) certified or eligible, dependable, flexible in scheduling demands, adept in all aspects of routine Histology inclusive of Frozen sections and willing to work in a fast paced,same-day turn-around-time based business. Our staff of 6, soon to be 7, Pathologists are Board Trained GI Pathologists, dedicated to our Client's and patient's needs as well as ours. Continuing Education is fully supported and encouraged. GI Pathology provides excellent benefits; 401K, life, health, and dental insurance, cafeteria plan, and bonus structure. Willing to assist on relocation expenses. If interested, please mail or e-mail resume to: Histology Dept attn: Marilyn McDonald 660 Jefferson Ave. Memphis, TN 38105 or m.mcdonald@gipath.com