From j.p.g.ooms <@t> zonnet.nl Sun Feb 1 04:13:42 2004 From: j.p.g.ooms <@t> zonnet.nl (Hans Ooms) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] decalcification of crista-biopsies Message-ID: <000e01c3e8ac$12874620$e963a63e@st2c8pdli7fy3i> Dear techs, scientists or whatsoever, The method of decalcifying bony tissue (cristabiopsies, femurheadslices etc.) I have experience with is by putting the specimen in a 5% sulphosalicylic acidsolution (or EDTA). The problem is: "How long should decalcification last to preserve morphology ?". I don't know a method of control. If the biopsie "feels like a sponge" it was taken out and routine processing (dehydration/alcoholrange, clearing/xylene and paraffinimpregnation in a VIP) was performed. Nearly every time morphology was awful ! Crista-biopsies are usually processed by means of MMA-resin (great morphology!) but this would be to expensive for all bony tissues. Despite Benno Romeis, Gurr, Junqueira I couldn't find anything satisfactory !! Someone out there able to help? Thanks in advance. Hans Ooms (HT/The Netherlands). From nick.kirk3 <@t> btopenworld.com Sun Feb 1 04:47:27 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] decalcification of crista-biopsies In-Reply-To: <000e01c3e8ac$12874620$e963a63e@st2c8pdli7fy3i> Message-ID: Hans Probably the best way of determining the end point of decalcification is the Ammonium hydroxide/ammonium oxalate method as detailed below. SOLUTIONS: Solution A, 5% aqueous Ammonium Hydroxide Solution B, 5% aqueous Ammonium Oxalate METHOD: Pipette 5 ml solution A into suitably sized flask or tube. Add 5 ml of solution B Add 5 ml of solution from the bottom of decalcification vessel. (Avoid picking up particulates in the pipette.) Let stand 15 minutes. If the test aliquot is clear, decalcification is completed and solution is still usable. If cloudy, the solution is exhausted, indicated by the precipitated calcium oxalate, and decalcification is not complete. Change the fluid and proceed, testing frequently. Hope that's of some use. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Hans Ooms Sent: 01 February 2004 10:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of crista-biopsies Dear techs, scientists or whatsoever, The method of decalcifying bony tissue (cristabiopsies, femurheadslices etc.) I have experience with is by putting the specimen in a 5% sulphosalicylic acidsolution (or EDTA). The problem is: "How long should decalcification last to preserve morphology ?". I don't know a method of control. If the biopsie "feels like a sponge" it was taken out and routine processing (dehydration/alcoholrange, clearing/xylene and paraffinimpregnation in a VIP) was performed. Nearly every time morphology was awful ! Crista-biopsies are usually processed by means of MMA-resin (great morphology!) but this would be to expensive for all bony tissues. Despite Benno Romeis, Gurr, Junqueira I couldn't find anything satisfactory !! Someone out there able to help? Thanks in advance. Hans Ooms (HT/The Netherlands). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Feb 1 11:37:47 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Immunohistochemistry - Micobacterium Message-ID: In my experience, IHC for mycobacteria is very sensitive, but not specific. Unfortunately, mycobacteria share antigens with other bacteria. Therefore, it is unlikely that one can generate an antibody that will only label mycobacteria, let alone an individual species. Yes, I use IHC to identify mycobacteria in fixed tissue, but the immunoreactivity must be confirmed with another technique if equivocal. There are several articles available on the IHC detection of mycobacteria in formalin-fixed tissue. In fact, the early articles date back to the early 1980's. Richard Cartun >>> "Darlene Jones" 01/28/04 08:59AM >>> I am new to the Histonet World, so hello everyone, Would anyone have any suggestions as to where I could find a source of a immunohistochemistry protocol for Micobacterium on the avian subspecies paratuberculosis? Including reagent and antibody sources? Thank-you, Darlene Jones, BSc. Necropsy & Research Technician Department of Pathology & Microbiology Atlantic Veterinary College, U.P.E.I. Phone:(902)628-4314 Fax:(902)566-0851 E-mail:djones@upei.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SCheasty <@t> ahs.llumc.edu Sun Feb 1 15:46:40 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Justification for Tape Coverslipper Message-ID: <2E50F33F91EEDA46A77BC3B2575BB091058757@mars.llumc.edu> The staff here can hand coverslip 30 slides in 8-10 minutes The Sakura tape coverslipper does 30 in 1.5 minutes. With our workload this is about 2 hours a day in tech time that would be saved. When this tech time cost savings is weighed against the cost of the machine and the increase in consumables (tape vs. coverslips and mounting medium) the savings does not seem that overwhelming, even over 5 years. Am I missing some factor in this equation? I truly love the Sakura coverslipper, have used it in two other labs, and even wrote a justification for one, but that was in 1992. Has the cost of the tape gone up that much? I remember a much bigger savings. Is my memory failing? Any input you experts can give me would be much appreciated! (except those who blame my failing memory...) Thanks Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From maovel70 <@t> hotmail.com Sun Feb 1 22:15:52 2004 From: maovel70 <@t> hotmail.com (mauricio velandia) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] help with criostat HM 505 E Message-ID: Hello to every body, excuse me for my english, it`s no very good. I need your help, I have a criostat Microm HM 505 E, but I haven`t service or user manual and when I turn on the equipment appear error E-03,and I don?t know what the meaning this error code, if is possible and somebody can help me say me what is this error?, Thanks you. Maurice Velandia technics Bogot? D.C. - Colombia _________________________________________________________________ Consigue aqu? las mejores y mas recientes ofertas de trabajo en Am?rica Latina y USA: http://latam.msn.com/empleos/ From maovel70 <@t> hotmail.com Sun Feb 1 22:21:16 2004 From: maovel70 <@t> hotmail.com (mauricio velandia) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] help with criostat HM 505 E Message-ID: Excuse me I don?t say what is my e-mail to answer about error Code E-03 in Hm 505 E of Microm criostat. My e-mail is maovel70@hotmail.com Thanks for your answers. _________________________________________________________________ MSN Amor: busca tu ? naranja http://latam.msn.com/amor/ From mikael.niku <@t> helsinki.fi Mon Feb 2 01:47:00 2004 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] combined ISH/IHC In-Reply-To: <5.2.0.9.2.20040128155027.00b64008@pop.nt.ntnu.no> Message-ID: <000101c3e960$c0dcbd90$8c0fd680@ekk1116> Dear Tora, we are routinely doing combined ISH + IHC. The ISH is for genomic DNA, so with a RNA target this might be slightly more tricky. But RNA in tissues is surprisingly well preserved, so I think this should work. I spent a LOT of time trying to find a useful protocol for a double staining. The only generally useful way I know is to use the tyramide signal amplification system. This is how it goes: 1) Start with IHC: antigen retrieval, primary antibody, biotinylated / HRP-conjugated secondary antibody, and then the tyramide reaction. Now you have a covalently bound label (biotinylated tyramide, for example) in the tissue, so you don't need to worry about antigen destruction or stripping of bound antibodies. 2) Then perform the whole ISH procedure. 3) After you have completed the NBT/BCIP color reaction of ISH, finish the IHC (if using biotinylated tyramide, add HRP-avidin, then DAB). This protocol allows you to begin with IHC (to avoid destruction of antigens in the harsh ISH treatments) but to do the visualization step only after ISH (to avoid false negatives caused by the DAB precipitate). As additional bonus, you get signal amplification for IHC, and a nicely even DAB color intensity (nice provided that you don't need any idea of quantitation). If it's the RNA that is the more sensitive target, I guess you could do this the other way round (to use tyramide for RNA detection). The only drawbacks I can think of are: 1) A few additional incubations due to the tyramide protocol (these are quick to do, though) 2) The commercial tyramide reagents (which you need to use at least if you're doing any commercial stuff, as the technology is patented) are pretty expensive, if you're doing lots of slides. Please let me know if you would like to get a copy of our exact protocol. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ + Mikael Niku + University of Helsinki, Dept. Basic Veterinary Sciences + URL: www.helsinki.fi/~mniku/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! (Gandhi) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of Tora Bardal > Sent: 28. tammikuuta 2004 17:16 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] combined ISH/IHC > > > Hello > Does anyone have a working protocol they can share? > > I'm trying to combine in situ hybridization (RNAprobe) and > immunohistochemistry (PCNA) on fish paraffin sections. > So far: ISH ok, IHC doesn't work, or ISH no signal, IHC ok. > PCNA(DAKO) > needs AR. > > Before spending more time an money I would like to have some > good advices > on what to do when, ab simultaneous incubation? stepwise? > crossreactions? > blocking ? > I'm using sheep anti-DIG-AP/BCIP/NBT (ISH) and DAKO Envision > HRP (mouse)/DAB. > > Tora > > > Tora Bardal > Department of Biology, > Norwegian University of Science and Technology (NTNU) > Bratt?ra Research Center > N-7491 Trondheim > Norway + (47)73 59 09 38 / 970 25256 > E-mail: Tora.Bardal@bio.ntnu.no fax:+ (47)73 59 63 11 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listin> fo/histonet > From John.Auld <@t> whnt.nhs.uk Mon Feb 2 03:35:03 2004 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: IHC validation Message-ID: Joyce In the UK there is a National EQA scheme, which also has non UK based labs enrolled, for IHC. There are several schemes run by the body, eg general, lymphoma, cytology, breast etc. EQA participation is a requirement for accreditation in the UK and if the inspectors think we should be in more or different schemes will register this as a non compliance. The EQA body also run meetings and workshops. John Date: Fri, 30 Jan 2004 14:18:18 -0500 From: "Joyce Cline" Subject: [Histonet] Validation for IHC To: Message-ID: <002001c3e765$d180d3e0$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="iso-8859-1" Does anyone send their positive control tissue to another lab for validation of their immuno staining? The path that is over the immuno area wants me to varify the use of my patient controls by sending to another lab to show that these controls are positive by another source. Has anyone been CAP inspected and asked if they do this? Seems he thinks an inspector is going to ask this. From Tony.Fearn <@t> cd.burnleyhc-tr.nwest.nhs.uk Mon Feb 2 05:37:47 2004 From: Tony.Fearn <@t> cd.burnleyhc-tr.nwest.nhs.uk (Fearn Tony) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] BCl2 on Ventana Benchmark Message-ID: <8C3DE9069A06D611B8950002A550C15943BD4E@BHC_MAIL02> We've been trying to optimise our antibodies on our Ventana Benchmark. At the moment we've been trying to use the DAKO antibody but it's not been that fantastic. Does anyone use the Ventana one, and is it any good? Lesley From CarringtonL <@t> Cardiff.ac.uk Mon Feb 2 06:57:25 2004 From: CarringtonL <@t> Cardiff.ac.uk (Louise Carrington) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Wanted dead or alive - tissue negative for connexin 43 Message-ID: hello , I am trying to find a tissue negative for connexin 43 (Cx43) to preadsorb an antibody with and/or use as a negative staining tissue - i have been using it to immuno-stain fresh-frozen and frozen/fixed (ethanol, acetone, 1%, 4% pfa) and have ended up with nuclear staining in rat, human and bovine tissue. it looks 'real' ie heterogenous cell-cell staining, its not my secondary - as ommission of primary yeilds no background (i'm using alexafluor fluorescent secondaries) or my fixation as all fixation prtocols including no-fix show the same and i've found it in several differnet organs (whilst searching for a positive control) in several different species...... but the antibody is to a phosphorylated isoform of cx43 and my my pan connexin 43 antibody shows no nuclear staining at all. The phospho-cx43 has not been published yet and the company have been ignoring my emails so if anyone can suggest a tissue (or even cell culture as i get staining here as well) that has nucleate Connexin43 cells within it that are either a) abundant and easy to isolate so that i can preadsorb the antibody OR b) easy to recognise so that i can do immunostaining (or god help me ... both??) i would be really grateful, i have been searching but cx 43 is a tad ubiquitous :) thankyou in advance louise Louise Carrington PhD. Cell & Molecular Unit, Department of Optometry & Vision Sciences, Cardiff University, Redwood Building, King Edward VII Ave., Cathays Park, Cardiff, Wales, UK Tel 02920 875665 email: CarringtonL@cardiff.ac.uk From juan.gutierrez <@t> christushealth.org Mon Feb 2 08:12:18 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] BCl2 on Ventana Benchmark Message-ID: Yes we do, and yes it is. We use CC1 standard, 28 min. primary and A/B block. Comes out beatiful. Good luck, Juan -----Original Message----- From: Fearn Tony [mailto:Tony.Fearn@cd.burnleyhc-tr.nwest.nhs.uk] Sent: Mon 2/2/2004 5:37 AM To: Histonet (E-mail) Cc: Subject: [Histonet] BCl2 on Ventana Benchmark We've been trying to optimise our antibodies on our Ventana Benchmark. At the moment we've been trying to use the DAKO antibody but it's not been that fantastic. Does anyone use the Ventana one, and is it any good? Lesley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From L.D.Ridley <@t> bham.ac.uk Mon Feb 2 08:20:37 2004 From: L.D.Ridley <@t> bham.ac.uk (LD Ridley) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] TMA Software. Message-ID: <1075731637.af1d4400L.D.Ridley@bham.ac.uk> This is just an enquiry to all those out there that construct and analyse Tissue Microarrays in the lab. We are looking into the various analysis software packages availible to try and organise our data we are rapidly generating. I would be really grateful if people could let me know what they are using, where it can be obtained from including costs etc and any other opinions you have of it. Many thanks in advance for your help Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 From L.D.Ridley <@t> bham.ac.uk Mon Feb 2 08:25:50 2004 From: L.D.Ridley <@t> bham.ac.uk (LD Ridley) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] TMA Software. Message-ID: <1075731950.af1d4400L.D.Ridley@bham.ac.uk> This is just an enquiry to all those out there that construct and analyse Tissue Microarrays in the lab. We are looking into the various analysis software packages availible to try and organise our data we are rapidly generating. I would be really grateful if people could let me know what they are using, where it can be obtained from including costs etc and any other opinions you have of it. Many thanks in advance for your help Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 From hfedor <@t> jhmi.edu Mon Feb 2 08:45:17 2004 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] TMA Software. Message-ID: http://webhost5.nts.jhu.edu/~tmalab/ this is our website. We have our own software, Which was designed and written by our lab. It is pretty user friendly. The data for the array design is put into the array builder application. After the slides are stained they can be scanned with an image analysis system, and the images are then directly linked to the data from the scan. you can get to our software from this page. you can view a demo of the software and also log on as guest to view images. Please let me know if you need any further assistance. Helen Helen L. Fedor B.S. Johns Hopkins University Brady Urological Institute 600 N Wolfe St Marburg Room 406 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 >>> LD Ridley 02/02/04 09:25AM >>> This is just an enquiry to all those out there that construct and analyse Tissue Microarrays in the lab. We are looking into the various analysis software packages availible to try and organise our data we are rapidly generating. I would be really grateful if people could let me know what they are using, where it can be obtained from including costs etc and any other opinions you have of it. Many thanks in advance for your help Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From browning <@t> HHSC.CA Mon Feb 2 08:48:32 2004 From: browning <@t> HHSC.CA (Browning Deb) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Justification for Tape Coverslipper Message-ID: <3AADFB88753AD31189C100902786B91C0E277FE6@hch_nt_exchange.hhsc.ca> Our main concern, while we do find the tape expensive, is the fact that the tape coverslips "remove" themselves after a few years, often removing the entire section with it, leaving us with drawers full of blank slides and loose coverslip/tissue combo's. At this point we need to recut the original block. The beauty of this system is that we can ship our slides within minutes of coverslipping. -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Sunday, February 01, 2004 4:47 PM To: HistoNet (E-mail) Subject: [Histonet] Justification for Tape Coverslipper The staff here can hand coverslip 30 slides in 8-10 minutes The Sakura tape coverslipper does 30 in 1.5 minutes. With our workload this is about 2 hours a day in tech time that would be saved. When this tech time cost savings is weighed against the cost of the machine and the increase in consumables (tape vs. coverslips and mounting medium) the savings does not seem that overwhelming, even over 5 years. Am I missing some factor in this equation? I truly love the Sakura coverslipper, have used it in two other labs, and even wrote a justification for one, but that was in 1992. Has the cost of the tape gone up that much? I remember a much bigger savings. Is my memory failing? Any input you experts can give me would be much appreciated! (except those who blame my failing memory...) Thanks Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From Luis.Chiriboga <@t> med.nyu.edu Mon Feb 2 09:06:01 2004 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] TMA Software. In-Reply-To: <1075731637.af1d4400L.D.Ridley@bham.ac.uk> Message-ID: Chih Long Lui etal American journal of Pathology V161(5)Nov02 1557-1565 Just started using & so far not to bad. software is based on gene microarray analysis so it's pretty complimentary. can get more info from their website. It's freeware. http://genome-www.stanford.edu/TMA/index.shtml Luis -------------------------------------- Luis Chiriboga Ph.D., HT (ASCP) QIHC New York University School Of Medicine NYU Cancer Institute and Bellevue Hospital Center Department Of Pathology 4W27 27th Street & First Avenue New York, N.Y. 10016 (212) 562-4667. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LD Ridley Sent: Monday, February 02, 2004 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TMA Software. This is just an enquiry to all those out there that construct and analyse Tissue Microarrays in the lab. We are looking into the various analysis software packages availible to try and organise our data we are rapidly generating. I would be really grateful if people could let me know what they are using, where it can be obtained from including costs etc and any other opinions you have of it. Many thanks in advance for your help Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Mon Feb 2 08:50:59 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:31 2005 Subject: Fwd: [Histonet] pregnancy in histology lab Message-ID: <6.0.1.1.0.20040202084903.025ba2a0@mailhost.vetmed.auburn.edu> >Date: Fri, 30 Jan 2004 12:38:05 -0800 (PST) >From: Stacey Burton >To: histonet@pathology.swmed.edu >X-Scan-Signature: 6696ac9d93c496fe8014a1b98c524a58 >X-Content-Filtered-By: Mailman/MimeDel 2.1.3 >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 976d3788468f6168a965852c1e2d4d6f >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] pregnancy in histology lab >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.2 required=6.5 tests=RCVD_IN_NJABL,RCVD_IN_SORBS > autolearn=no version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes > >I am performing a pole for some inquiring employees. If anyone would like >to comment - Thank you. > >1. Have you ever been pregnant while working with in the histology >laboratory? Yes, twice. > >2. Did your work duties have to be altered while being pregnant and to >what extent? No. > >3. Did working in the histology laboratory effect your pregnancy in any >negative way? No. > >Our laboratory does perform regular ppm testing for xylene and formalin. > >Thank you for any comments for our pole, > >Stacey L. Burton, H.T. ASCP >Laboratory Manager >Unipath LLC >San Antonio TX >210) 521-7700 >staceylburton@yahoo.com > > > > > >--------------------------------- >Do you Yahoo!? >Yahoo! SiteBuilder - Free web site building tool. Try it! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From Janet.Bonner <@t> FLHOSP.ORG Mon Feb 2 09:28:39 2004 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] pregnancy in histology lab Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F4B@fh2k093.fhmis.net> I think that if anything develops later on in your children, you'll always wonder what role Histology may have played. I worked in a lab in 1980-81 when I was pregnant and my son seemed fine - until last week. An Echo cardiogram showed a missing tricuspid valve and the other two were fused! Of course now he's 22 and been through things like strept throat and ear aches and God only knows what other flus, genetic abnormalities never discovered before ECHOs, etc. Enjoy life while we have it together, but ALWAYS take precautions no matter what you are doing- (My older collegues suffered as a result of choloroform which used to be widely used in Histology but is now restricted/prohibited.) -----Original Message----- From: Gudrun Lang [mailto:gudrun.lang@aon.at] Sent: Friday, January 30, 2004 5:27 PM To: Histonetliste; Stacey Burton Subject: Re: [Histonet] pregnancy in histology lab My children are 8 and 11 years old, and until now they are obviosly healthy. During pregnancy I was not allowed to be in rooms with formalin, xylol or any other probably cancerogen reagens. I was not allowed to handle with nativ probes, like making cryo sections or grossing. My duties were cutting in a seperate room and doing administrative jobs. I had a coworker, who had her children in the 1960s. In this time, there were no fumehoods and xylol-wet slides were dried near the radiator. Using gloves was not really appriciated. But yet her girls are healthy and have also healthy children. But something irritated me, when she told about the good old times. Her elder colleages didn't reach a really high age and often had some cancer disease?! hope this helps Gudrun Lang Linz, Austria ----- Original Message ----- From: "Stacey Burton" To: Sent: Friday, January 30, 2004 9:38 PM Subject: [Histonet] pregnancy in histology lab > I am performing a pole for some inquiring employees. If anyone would like to comment - Thank you. > > 1. Have you ever been pregnant while working with in the histology laboratory? > > 2. Did your work duties have to be altered while being pregnant and to what extent? > > 3. Did working in the histology laboratory effect your pregnancy in any negative way? > > Our laboratory does perform regular ppm testing for xylene and formalin. > > Thank you for any comments for our pole, > > Stacey L. Burton, H.T. ASCP > Laboratory Manager > Unipath LLC > San Antonio TX > 210) 521-7700 > staceylburton@yahoo.com > > > > > > --------------------------------- > Do you Yahoo!? > Yahoo! SiteBuilder - Free web site building tool. Try it! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From hborgeri <@t> wfubmc.edu Mon Feb 2 09:29:29 2004 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Antibody search Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8EA13@EXCHVS2.medctr.ad.wfubmc.edu> Hi all, One of our research investigators is looking for two antibodies: (1) Steroid sulfatase and (2) 17 beta hydroxysteroid dehydrogenase. I did an online search but came up with nothing. He was also interested in knowing if any of you have successfully used Cytochrome P450 Aromatase from Serotec on FFPE tissues. I would greatly appreciate any input you might be able to give me. Hermina Hermina M. Borgerink, BA, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax (336) 716-1515 e-mail hborgeri@wfubmc.edu From Jackie.O'Connor <@t> abbott.com Mon Feb 2 10:08:36 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: pregnancy in lab Message-ID: I've been working in histology since 1970. I didn't work in a lab during my first two pregnancies - my oldest daughter (25) and second daughter (24) were biology and art majors, respectively. I worked during my 3rd pregnancy (1982) in a tox research lab with no safety measures except for an exhaust hood for formalin trimming and xylene coverslipping. A couple of the tox PhD's suggested I stay away from xylene during my pregnancy, so someone else coverslipped for me - I still did the staining. That daughter was diagnosed with learning disabilities when she was 8 years old, and she continues to have problems at age 21. While working full time in a lab where we only had laminar flow ventilation to draw away xylene and formalin fumes, my fourth pregnancy ended in a fetal demise at 20 weeks. I worked full time during my next pregnancy, (1984-1985). That daughter has multiple minor congenital defects which include a mitral valve prolapse, scoliosis, a concave sternum, a small area of left temporal lobe atrophy, and a mandible deformity. She also is plagued with learning disabilities. Two subsequent pregnancies (1987, 1989) both resulted in second trimester fetal demise. My high risk OB in Chicago suggested at the time (1989) that lab chemicals may be suspect in my case, and when it ended he said "You're not going to try this again, are you?" As I've heard other people state, you just never know. I'll never know. There are other chemicals in the lab to consider as well as formalin and xylene, tho - aniline dyes, silver nitrate, mercury, uranyl nitrate, chloroform, toluene, to name a few. Personally, I would recommend any pregnant woman stay out of the histology lab. I wish the NSH would do a study on pregnancy in the histology lab, retrospective or otherwise. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 From algranth <@t> u.arizona.edu Mon Feb 2 10:46:45 2004 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: pregnancy in lab In-Reply-To: Message-ID: <4.3.2.7.2.20040202092832.00cafee0@algranth.inbox.email.arizona.edu> My children are 29 and 26. I wasn't working in Histology when I was pregnant the first time. This child has a degree in Molecular Biology and is working in a cord blood registry lab, however despite an exceptionally high IQ he was diagnosed with several learning disabilities while in elementary and jr. high and suffered from allergies to just about everything. I know it is very controversial now but ritalin was our salvation along with a very busy schedule. The medication didn't make him a zombie and it was an incredible help to him in the academic setting. Thankfully he now does better with the allergies and he does well in work situations. My second child is an archeologist with a masters degree and suffered only from being an "average" child. I was working in histology when I was pregnant with her. I asked the pathologists and lab manager during that time about the hazards of working around the chemicals and they told me not to be concerned - their only concern was how long the maternity leave was going to be! I took what precautions I could - it was a lot different back then. She was born a month early but suffered no serious effects. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Rcartun <@t> harthosp.org Mon Feb 2 11:13:16 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Slide labeling pens Message-ID: I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass slides. Unfortunately, our supplier no long carries them. I was pleased to see that "Ted Pella, Inc." carries them. However, I spoke with them this morning and they told me that they are currently NOT available. Does anyone know a supplier for these pens? Thanks! Richard Cartun From HornHV <@t> archildrens.org Mon Feb 2 11:27:08 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: "Need your help" Message-ID: Our hospital has a surgical case review committee as well. We don't to the reqs for no specimens though. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: MTitford@aol.com [mailto:MTitford@aol.com] Sent: Friday, January 30, 2004 4:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: "Need your help" Nita Searcy asks about investigating possible unneeded surgical procedures. Our hospital, as part of its JCAHO accreditation (Joint Council for the Accretation of Hospitals Organization or something close to that)has a "Surgical Case Review Committee". It meets monthly and reviews suspect or unnecessary surgery's among other things. It also takes on little projects like reviewing appendix operations to make sure they are needed. Also as part of our accretation processes, we require a surgical pathology slip be issued for each surgery that takes place, even if there is no specimen. The idea is the pathologist reviews the slip and if he/she thinks it is unnecessary,or there should have been a specimen, the slip is sent on to the "Surgical Case Review Committee". The scrub nurse signs these off as "none" where the specimen should be listed. (I served on the committe for a couple of years. It was quite interesting. We sat in plush chairs in the hospital boardroom and got fed lunch....) Mike Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From garygill <@t> dcla.com Mon Feb 2 11:28:03 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Slide labeling pens Message-ID: Try Google, 86 hits for redisharp plus. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Monday, February 02, 2004 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling pens I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass slides. Unfortunately, our supplier no long carries them. I was pleased to see that "Ted Pella, Inc." carries them. However, I spoke with them this morning and they told me that they are currently NOT available. Does anyone know a supplier for these pens? Thanks! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbecker <@t> pathlabinc.com Mon Feb 2 11:43:30 2004 From: mbecker <@t> pathlabinc.com (Michelle Becker) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Slide labeling pens In-Reply-To: Message-ID: Try Staples #498428 for fine black ink - $13.08 per dozen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Monday, February 02, 2004 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling pens I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass slides. Unfortunately, our supplier no long carries them. I was pleased to see that "Ted Pella, Inc." carries them. However, I spoke with them this morning and they told me that they are currently NOT available. Does anyone know a supplier for these pens? Thanks! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epravda <@t> forsyth.org Mon Feb 2 11:56:53 2004 From: epravda <@t> forsyth.org (Pravda, Elke) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Image Analysis Software Message-ID: <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org> We have a post-doc in our lab that is looking for a Windows-based software for image analysis, specifically pixel analysis, of his confocal images. He is doing colocalization studies of his fluorescent-labeled tissue and is looking for a program that is less than $500 that he can load on his personal computer. We have Image-Pro Plus in the lab, but I am not familiar with other programs (let alone this one) and was wondering if anyone has any suggestions for us. Thanks so much!! Elke Pravda Research Assistant Biostructure Core Facility Forsyth Institute Boston, MA 02155 From spoulos <@t> saa.ars.usda.gov Mon Feb 2 12:26:37 2004 From: spoulos <@t> saa.ars.usda.gov (Sylvia Poulos) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] pregnancy in lab Message-ID: My comment is not specific to pregnancy and lab work but the lab environment in general. I once worked in a building where several individuals in one lab were continuously ill with anything from chronic fatigue to renal and liver failure. The employer stripped the lab, put in new tiles, new lab benches, cleaned and repainted the walls, etc,to no avail. In the end, the problem ended up being airflow in the building and "things" were entering the lab via the air ducts. My point? Although you do everything in your power to stay safe in the lab, you don't know what others in the area are doing (knowingly or unknowingly) and how that will affect you. Sylvia P. Poulos USDA-ARS-Animal Physiology Research Unit Athens, GA 30605 706-583-8279 706-542-0399 (fax) From sthevar <@t> bu.edu Mon Feb 2 12:33:59 2004 From: sthevar <@t> bu.edu (Sandy Thevarkunnel) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: help with criostat HM 505 E (mauricio velandia) In-Reply-To: Message-ID: <5E4D4DF4-55AE-11D8-98F3-00039376BC40@bu.edu> Hi, We had that problem when someone from another lab turned off our cryostat. There should be a blank button on the control panel probably under the lightbulb button. You just need to press that and it should restart it. The cryostat needs to cool down that's why the error message occurs. hope this helps Sandy Thevarkunnel Boston University School of Medicine On Monday, February 2, 2004, at 01:00 PM, histonet- request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Justification for Tape Coverslipper (Cheasty, Sandra) > 2. help with criostat HM 505 E (mauricio velandia) > 3. help with criostat HM 505 E (mauricio velandia) > 4. RE: combined ISH/IHC (Mikael Niku) > 5. Re: IHC validation (John Auld) > 6. BCl2 on Ventana Benchmark (Fearn Tony) > 7. Wanted dead or alive - tissue negative for connexin 43 > (Louise Carrington) > 8. RE: BCl2 on Ventana Benchmark (GUTIERREZ, JUAN) > 9. TMA Software. (LD Ridley) > 10. TMA Software. (LD Ridley) > 11. Re: TMA Software. (Helen Fedor) > 12. RE: Justification for Tape Coverslipper (Browning Deb) > 13. RE: TMA Software. (Luis Chiriboga) > 14. Fwd: [Histonet] pregnancy in histology lab (Atoska S. Gentry) > 15. RE: pregnancy in histology lab (Bonner, Janet) > 16. Antibody search (Hermina Borgerink) > 17. Re: pregnancy in lab (Jackie.O'Connor@abbott.com) > 18. Re: Re: pregnancy in lab (Andrea Grantham) > 19. Slide labeling pens (Richard Cartun) > 20. RE: Re: "Need your help" (Horn, Hazel V) > 21. RE: Slide labeling pens (Gary Gill) > 22. RE: Slide labeling pens (Michelle Becker) > 23. Image Analysis Software (Pravda, Elke) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 1 Feb 2004 13:46:40 -0800 > From: "Cheasty, Sandra" > Subject: [Histonet] Justification for Tape Coverslipper > To: "HistoNet \(E-mail\)" > Message-ID: <2E50F33F91EEDA46A77BC3B2575BB091058757@mars.llumc.edu> > Content-Type: text/plain; charset="iso-8859-1" > > The staff here can hand coverslip 30 slides in 8-10 minutes > The Sakura tape coverslipper does 30 in 1.5 minutes. > With our workload this is about 2 hours a day in tech time that would > be saved. > When this tech time cost savings is weighed against the cost of the > machine and the increase in consumables (tape vs. coverslips and > mounting medium) the savings does not seem that overwhelming, even over > 5 years. > > Am I missing some factor in this equation? I truly love the Sakura > coverslipper, have used it in two other labs, and even wrote a > justification for one, but that was in 1992. Has the cost of the tape > gone up that much? I remember a much bigger savings. Is my memory > failing? Any input you experts can give me would be much > appreciated! (except those who blame my failing memory...) > > Thanks > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains > information that may be confidential, protected by applicable legal > privileges, or constitute non-public information. It is intended to be > conveyed only to the designated recipient(s). If you are not an > intended recipient of this message, please notify the sender by > replying to this message and then delete it from your system. Use, > dissemination, distribution or reproduction of this message by > unintended recipients is not authorized and may be unlawful. > > > > > > ------------------------------ > > Message: 2 > Date: Sun, 01 Feb 2004 23:15:52 -0500 > From: "mauricio velandia" > Subject: [Histonet] help with criostat HM 505 E > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=iso-8859-1; format=flowed > > Hello to every body, excuse me for my english, it`s no very good. > > I need your help, I have a criostat Microm HM 505 E, but I haven`t > service > or user manual and when I turn on the equipment appear error E-03,and I > don?t know what the meaning this error code, if is possible and > somebody can > help me say me what is this error?, Thanks you. > > Maurice Velandia > technics > Bogot? D.C. - Colombia > > _________________________________________________________________ > Consigue aqu? las mejores y mas recientes ofertas de trabajo en Am?rica > Latina y USA: http://latam.msn.com/empleos/ > > > > > ------------------------------ > > Message: 3 > Date: Sun, 01 Feb 2004 23:21:16 -0500 > From: "mauricio velandia" > Subject: [Histonet] help with criostat HM 505 E > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=iso-8859-1; format=flowed > > Excuse me I don?t say what is my e-mail to answer about error Code E-03 > in > Hm 505 E of Microm criostat. > > My e-mail is maovel70@hotmail.com > > Thanks for your answers. > > _________________________________________________________________ > MSN Amor: busca tu ? naranja http://latam.msn.com/amor/ > > > > > ------------------------------ > > Message: 4 > Date: Mon, 2 Feb 2004 09:47:00 +0200 > From: "Mikael Niku" > Subject: RE: [Histonet] combined ISH/IHC > To: "'Tora Bardal'" , > > Message-ID: <000101c3e960$c0dcbd90$8c0fd680@ekk1116> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Tora, > > we are routinely doing combined ISH + IHC. The ISH is for genomic DNA, > so with a RNA target this might be slightly more tricky. But RNA in > tissues is surprisingly well preserved, so I think this should work. > > I spent a LOT of time trying to find a useful protocol for a double > staining. The only generally useful way I know is to use the tyramide > signal amplification system. > > This is how it goes: > > 1) Start with IHC: antigen retrieval, primary antibody, biotinylated / > HRP-conjugated secondary antibody, and then the tyramide reaction. Now > you have a covalently bound label (biotinylated tyramide, for example) > in the tissue, so you don't need to worry about antigen destruction or > stripping of bound antibodies. > > 2) Then perform the whole ISH procedure. > > 3) After you have completed the NBT/BCIP color reaction of ISH, finish > the IHC (if using biotinylated tyramide, add HRP-avidin, then DAB). > > This protocol allows you to begin with IHC (to avoid destruction of > antigens in the harsh ISH treatments) but to do the visualization step > only after ISH (to avoid false negatives caused by the DAB precipitate). > As additional bonus, you get signal amplification for IHC, and a nicely > even DAB color intensity (nice provided that you don't need any idea of > quantitation). > > If it's the RNA that is the more sensitive target, I guess you could do > this the other way round (to use tyramide for RNA detection). > > The only drawbacks I can think of are: > > 1) A few additional incubations due to the tyramide protocol (these are > quick to do, though) > 2) The commercial tyramide reagents (which you need to use at least if > you're doing any commercial stuff, as the technology is patented) are > pretty expensive, if you're doing lots of slides. > > Please let me know if you would like to get a copy of our exact > protocol. > > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > + Mikael Niku > + University of Helsinki, Dept. Basic Veterinary Sciences > + URL: www.helsinki.fi/~mniku/ > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > > - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? > Minusta se olisi erinomainen ajatus! > (Gandhi) > > > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf >> Of Tora Bardal >> Sent: 28. tammikuuta 2004 17:16 >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] combined ISH/IHC >> >> >> Hello >> Does anyone have a working protocol they can share? >> >> I'm trying to combine in situ hybridization (RNAprobe) and >> immunohistochemistry (PCNA) on fish paraffin sections. >> So far: ISH ok, IHC doesn't work, or ISH no signal, IHC ok. >> PCNA(DAKO) >> needs AR. >> >> Before spending more time an money I would like to have some >> good advices >> on what to do when, ab simultaneous incubation? stepwise? >> crossreactions? >> blocking ? >> I'm using sheep anti-DIG-AP/BCIP/NBT (ISH) and DAKO Envision >> HRP (mouse)/DAB. >> >> Tora >> >> >> Tora Bardal >> Department of Biology, >> Norwegian University of Science and Technology (NTNU) >> Bratt?ra Research Center >> N-7491 Trondheim >> Norway + (47)73 59 09 38 / 970 25256 >> E-mail: Tora.Bardal@bio.ntnu.no fax:+ (47)73 59 63 11 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listin> fo/histonet >> > > > > > ------------------------------ > > Message: 5 > Date: Mon, 2 Feb 2004 09:35:03 +0000 > From: "John Auld" > Subject: [Histonet] Re: IHC validation > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Joyce > > In the UK there is a National EQA scheme, which also has non UK based > labs > enrolled, for IHC. There are several schemes run by the body, eg > general, > lymphoma, cytology, breast etc. EQA participation is a requirement for > accreditation in the UK and if the inspectors think we should be in > more or > different schemes will register this as a non compliance. The EQA body > also > run meetings and workshops. > > John > > Date: Fri, 30 Jan 2004 14:18:18 -0500 > From: "Joyce Cline" > Subject: [Histonet] Validation for IHC > To: > Message-ID: <002001c3e765$d180d3e0$1d2a14ac@wchsys.org> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone send their positive control tissue to another lab for > validation of their immuno staining? > The path that is over the immuno area wants me to varify the use > of my > patient controls by sending to another lab to show that these controls > are > positive by another source. > Has anyone been CAP inspected and asked if they do this? Seems he > thinks an inspector is going to ask this. > > > > > ------------------------------ > > Message: 6 > Date: Mon, 2 Feb 2004 11:37:47 -0000 > From: Fearn Tony > Subject: [Histonet] BCl2 on Ventana Benchmark > To: "Histonet (E-mail)" > Message-ID: <8C3DE9069A06D611B8950002A550C15943BD4E@BHC_MAIL02> > Content-Type: text/plain; charset="ISO-8859-1" > > We've been trying to optimise our antibodies on our Ventana Benchmark. > At > the moment we've been trying to use the DAKO antibody but it's not been > that > fantastic. Does anyone use the Ventana one, and is it any good? > Lesley > > > > > > ------------------------------ > > Message: 7 > Date: Mon, 02 Feb 2004 12:57:25 +0000 > From: "Louise Carrington" > Subject: [Histonet] Wanted dead or alive - tissue negative for > connexin 43 > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > hello , > I am trying to find a tissue negative for connexin 43 (Cx43) to > preadsorb an > antibody with and/or use as a negative staining tissue - i have been > using it to > immuno-stain fresh-frozen and frozen/fixed (ethanol, acetone, 1%, 4% > pfa) and > have ended up with nuclear staining in rat, human and bovine tissue. it > looks > 'real' ie heterogenous cell-cell staining, its not my secondary - as > ommission > of primary yeilds no background (i'm using alexafluor fluorescent > secondaries) > or my fixation as all fixation prtocols including no-fix show the same > and i've > found it in several differnet organs (whilst searching for a positive > control) > in several different species...... but the antibody is to a > phosphorylated > isoform of cx43 and my my pan connexin 43 antibody shows no nuclear > staining at > all. The phospho-cx43 has not been published yet and the company have > been > ignoring my emails so if anyone can suggest a tissue (or even cell > culture as i > get staining here as well) that has nucleate Connexin43 cells within it > that are > either > > a) abundant and easy to isolate so that i can preadsorb the antibody OR > > b) easy to recognise so that i can do immunostaining > > (or god help me ... both??) > i would be really grateful, > > i have been searching but cx 43 is a tad ubiquitous :) > > thankyou in advance > > louise > > > > Louise Carrington PhD. > Cell & Molecular Unit, > Department of Optometry & Vision Sciences, > Cardiff University, > Redwood Building, > King Edward VII Ave., > Cathays Park, > Cardiff, > Wales, > UK > > Tel 02920 875665 > email: CarringtonL@cardiff.ac.uk > > > > ------------------------------ > > Message: 8 > Date: Mon, 2 Feb 2004 08:12:18 -0600 > From: "GUTIERREZ, JUAN" > Subject: RE: [Histonet] BCl2 on Ventana Benchmark > To: "Fearn Tony" , "Histonet > (E-mail)" > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > Yes we do, and yes it is. We use CC1 standard, 28 min. primary and A/B > block. Comes out beatiful. Good luck, > > Juan > > -----Original Message----- > From: Fearn Tony [mailto:Tony.Fearn@cd.burnleyhc-tr.nwest.nhs.uk] > Sent: Mon 2/2/2004 5:37 AM > To: Histonet (E-mail) > Cc: > Subject: [Histonet] BCl2 on Ventana Benchmark > > > > We've been trying to optimise our antibodies on our Ventana > Benchmark. At > the moment we've been trying to use the DAKO antibody but it's not > been that > fantastic. Does anyone use the Ventana one, and is it any good? > Lesley > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Mon, 02 Feb 2004 14:20:37 +0000 > From: LD Ridley > Subject: [Histonet] TMA Software. > To: histonet@lists.utsouthwestern.edu > Message-ID: <1075731637.af1d4400L.D.Ridley@bham.ac.uk> > Content-Type: text/plain; charset="UTF-8" > > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are rapidly generating. I would be really grateful if people could let > me know what they are using, where it can be obtained from including > costs etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > > > ------------------------------ > > Message: 10 > Date: Mon, 02 Feb 2004 14:25:50 +0000 > From: LD Ridley > Subject: [Histonet] TMA Software. > To: histonet@lists.utsouthwestern.edu > Message-ID: <1075731950.af1d4400L.D.Ridley@bham.ac.uk> > Content-Type: text/plain; charset="UTF-8" > > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are rapidly generating. I would be really grateful if people could let > me know what they are using, where it can be obtained from including > costs etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > > > ------------------------------ > > Message: 11 > Date: Mon, 02 Feb 2004 09:45:17 -0500 > From: Helen Fedor > Subject: Re: [Histonet] TMA Software. > To: L.D.Ridley@bham.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > http://webhost5.nts.jhu.edu/~tmalab/ > this is our website. > We have our own software, Which was designed and written by our lab. > It is pretty user friendly. The data for the array design is put into > the array builder application. After the slides are stained they can be > scanned with an image analysis system, and the images are then directly > linked to the data from the scan. > you can get to our software from this page. > you can view a demo of the software and also log on as guest to view > images. > Please let me know if you need any further assistance. > > Helen > > > Helen L. Fedor B.S. > Johns Hopkins University > Brady Urological Institute > 600 N Wolfe St > Marburg Room 406 > > WARNING: E-mail sent over the Internet is not secure. Information sent > by e-mail may not remain confidential. > DISCLAIMER: This e-mail is intended only for the individual to whom it > is addressed. It may be used only in accordance with applicable laws. If > you received this e-mail by mistake, notify the sender and destroy the > e-mail. > > Baltimore MD 21287 > email: hfedor@jhmi.edu > Phone: 410 614-1660 > Pager: 410 283-3419 > > >>>> LD Ridley 02/02/04 09:25AM >>> > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we are > rapidly generating. I would be really grateful if people could let me > know what they are using, where it can be obtained from including costs > etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Mon, 2 Feb 2004 09:48:32 -0500 > From: Browning Deb > Subject: RE: [Histonet] Justification for Tape Coverslipper > To: "'Cheasty, Sandra'" , "HistoNet (E-mail)" > > Message-ID: > <3AADFB88753AD31189C100902786B91C0E277FE6@hch_nt_exchange.hhsc.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Our main concern, while we do find the tape expensive, is the fact that > the > tape coverslips "remove" themselves after a few years, often removing > the > entire section with it, leaving us with drawers full of blank slides and > loose coverslip/tissue combo's. At this point we need to recut the > original > block. The beauty of this system is that we can ship our slides within > minutes of coverslipping. > > -----Original Message----- > From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] > Sent: Sunday, February 01, 2004 4:47 PM > To: HistoNet (E-mail) > Subject: [Histonet] Justification for Tape Coverslipper > > > The staff here can hand coverslip 30 slides in 8-10 minutes > The Sakura tape coverslipper does 30 in 1.5 minutes. > With our workload this is about 2 hours a day in tech time that would be > saved. > When this tech time cost savings is weighed against the cost of the > machine > and the increase in consumables (tape vs. coverslips and mounting > medium) > the savings does not seem that overwhelming, even over 5 years. > > Am I missing some factor in this equation? I truly love the Sakura > coverslipper, have used it in two other labs, and even wrote a > justification > for one, but that was in 1992. Has the cost of the tape gone up that > much? > I remember a much bigger savings. Is my memory failing? Any input you > experts can give me would be much appreciated! (except those who > blame my > failing memory...) > > Thanks > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains > information that may be confidential, protected by applicable legal > privileges, or constitute non-public information. It is intended to be > conveyed only to the designated recipient(s). If you are not an intended > recipient of this message, please notify the sender by replying to this > message and then delete it from your system. Use, dissemination, > distribution or reproduction of this message by unintended recipients > is not > authorized and may be unlawful. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This information is directed in confidence solely to the person named > above > and may not otherwise be distributed, copied or disclosed. Therefore, > this > information should be considered strictly confidential. If you have > received this email in error, please notify the sender immediately via a > return email for further direction. Thank you for your assistance. > > > > > > ------------------------------ > > Message: 13 > Date: Mon, 02 Feb 2004 10:06:01 -0500 > From: Luis Chiriboga > Subject: RE: [Histonet] TMA Software. > To: LD Ridley , > histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=utf-8 > > Chih Long Lui etal American journal of Pathology V161(5)Nov02 1557-1565 > Just started using & so far not to bad. software is based on gene > microarray analysis so it's pretty complimentary. can get more info > from their website. It's freeware. > http://genome-www.stanford.edu/TMA/index.shtml > > Luis > > > -------------------------------------- > Luis Chiriboga Ph.D., HT (ASCP) QIHC > New York University School Of Medicine > NYU Cancer Institute and > Bellevue Hospital Center > Department Of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > (212) 562-4667. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LD Ridley > Sent: Monday, February 02, 2004 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TMA Software. > > > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are rapidly generating. I would be really grateful if people could let > me know what they are using, where it can be obtained from including > costs etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Mon, 02 Feb 2004 08:50:59 -0600 > From: "Atoska S. Gentry" > Subject: Fwd: [Histonet] pregnancy in histology lab > To: Histonet > Message-ID: > <6.0.1.1.0.20040202084903.025ba2a0@mailhost.vetmed.auburn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > >> Date: Fri, 30 Jan 2004 12:38:05 -0800 (PST) >> From: Stacey Burton >> To: histonet@pathology.swmed.edu >> X-Scan-Signature: 6696ac9d93c496fe8014a1b98c524a58 >> X-Content-Filtered-By: Mailman/MimeDel 2.1.3 >> Cc: >> X-BeenThere: histonet@lists.utsouthwestern.edu >> X-Mailman-Version: 2.1.3 >> List-Id: For the exchange of information pertaining to histotechnology >> and >> related fields >> List-Unsubscribe: >> , >> >> >> List-Archive: >> List-Post: >> List-Help: > request@lists.utsouthwestern.edu?subject=help> >> List-Subscribe: >> , >> > request@lists.utsouthwestern.edu?subject=subscribe> >> Sender: histonet-bounces@lists.utsouthwestern.edu >> X-Scan-Signature: 976d3788468f6168a965852c1e2d4d6f >> X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >> Subject: [Histonet] pregnancy in histology lab >> X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >> swlx162.swmed.edu >> X-Spam-Level: >> X-Spam-Status: No, hits=0.2 required=6.5 >> tests=RCVD_IN_NJABL,RCVD_IN_SORBS >> autolearn=no version=2.63 >> X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >> X-SA-Exim-Scanned: Yes >> >> I am performing a pole for some inquiring employees. If anyone would >> like >> to comment - Thank you. >> >> 1. Have you ever been pregnant while working with in the histology >> laboratory? Yes, twice. >> >> 2. Did your work duties have to be altered while being pregnant and to >> what extent? No. >> >> 3. Did working in the histology laboratory effect your pregnancy in >> any >> negative way? No. > >> >> Our laboratory does perform regular ppm testing for xylene and >> formalin. >> >> Thank you for any comments for our pole, >> >> Stacey L. Burton, H.T. ASCP >> Laboratory Manager >> Unipath LLC >> San Antonio TX >> 210) 521-7700 >> staceylburton@yahoo.com >> >> >> >> >> >> --------------------------------- >> Do you Yahoo!? >> Yahoo! SiteBuilder - Free web site building tool. Try it! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > > > ------------------------------ > > Message: 15 > Date: Mon, 2 Feb 2004 10:28:39 -0500 > From: "Bonner, Janet" > Subject: RE: [Histonet] pregnancy in histology lab > To: "'Gudrun Lang'" , Histonetliste > , Stacey Burton > > Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F4B@fh2k093.fhmis.net> > Content-Type: text/plain; charset="iso-8859-1" > > I think that if anything develops later on in your children, you'll > always > wonder what role Histology may have played. I worked in a lab in > 1980-81 > when I was pregnant and my son seemed fine - until last week. An Echo > cardiogram showed a missing tricuspid valve and the other two were > fused! > Of course now he's 22 and been through things like strept throat and ear > aches and God only knows what other flus, genetic abnormalities never > discovered before ECHOs, etc. Enjoy life while we have it together, > but > ALWAYS take precautions no matter what you are doing- > (My older collegues suffered as a result of choloroform which used to > be > widely used in Histology but is now restricted/prohibited.) > > -----Original Message----- > From: Gudrun Lang [mailto:gudrun.lang@aon.at] > Sent: Friday, January 30, 2004 5:27 PM > To: Histonetliste; Stacey Burton > Subject: Re: [Histonet] pregnancy in histology lab > > > My children are 8 and 11 years old, and until now they are obviosly > healthy. > During pregnancy I was not allowed to be in rooms with formalin, xylol > or > any other probably cancerogen reagens. I was not allowed to handle with > nativ probes, like making cryo sections or grossing. > My duties were cutting in a seperate room and doing administrative jobs. > I had a coworker, who had her children in the 1960s. In this time, there > were no fumehoods and xylol-wet slides were dried near the radiator. > Using > gloves was not really appriciated. But yet her girls are healthy and > have > also healthy children. > But something irritated me, when she told about the good old times. Her > elder colleages didn't reach a really high age and often had some cancer > disease?! > hope this helps > > Gudrun Lang > Linz, Austria > ----- Original Message ----- > From: "Stacey Burton" > To: > Sent: Friday, January 30, 2004 9:38 PM > Subject: [Histonet] pregnancy in histology lab > > >> I am performing a pole for some inquiring employees. If anyone would >> like > to comment - Thank you. >> >> 1. Have you ever been pregnant while working with in the histology > laboratory? >> >> 2. Did your work duties have to be altered while being pregnant and to > what extent? >> >> 3. Did working in the histology laboratory effect your pregnancy in >> any > negative way? >> >> Our laboratory does perform regular ppm testing for xylene and >> formalin. >> >> Thank you for any comments for our pole, >> >> Stacey L. Burton, H.T. ASCP >> Laboratory Manager >> Unipath LLC >> San Antonio TX >> 210) 521-7700 >> staceylburton@yahoo.com >> >> >> >> >> >> --------------------------------- >> Do you Yahoo!? >> Yahoo! SiteBuilder - Free web site building tool. Try it! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be privileged and/or > confidential and protected from disclosure. If the reader of this > message > is not the intended recipient or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify > the sender immediately by replying to this message and deleting the > material > from any computer. > > > > ------------------------------ > > Message: 16 > Date: Mon, 2 Feb 2004 10:29:29 -0500 > From: "Hermina Borgerink" > Subject: [Histonet] Antibody search > To: "histonet" > Message-ID: > <9AEEF1FB6254224AA355ED285F84916503F8EA13@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="US-ASCII" > > Hi all, > One of our research investigators is looking for two antibodies: (1) > Steroid sulfatase and (2) 17 beta hydroxysteroid dehydrogenase. I did > an online search but came up with nothing. He was also interested in > knowing if any of you have successfully used Cytochrome P450 Aromatase > from Serotec on FFPE tissues. I would greatly appreciate any input you > might be able to give me. > Hermina > > Hermina M. Borgerink, BA, HTL(ASCP)QIHC > Wake Forest University Health Sciences > Department of Pathology > Medical Center Blvd. > Winston-Salem, NC 27157 > Tel. (336) 716-1538 > Fax (336) 716-1515 > e-mail hborgeri@wfubmc.edu > > > > > ------------------------------ > > Message: 17 > Date: Mon, 2 Feb 2004 10:08:36 -0600 > From: Jackie.O'Connor@abbott.com > Subject: [Histonet] Re: pregnancy in lab > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > ON86256E2E.0056AC80@northamerica.intra.abbott.com> > > Content-Type: text/plain; charset="us-ascii" > > I've been working in histology since 1970. I didn't work in a lab > during > my first two pregnancies - my oldest daughter (25) and second daughter > (24) were biology and art majors, respectively. I worked during my 3rd > pregnancy (1982) in a tox research lab with no safety measures except > for > an exhaust hood for formalin trimming and xylene coverslipping. A > couple > of the tox PhD's suggested I stay away from xylene during my pregnancy, > so > someone else coverslipped for me - I still did the staining. That > daughter was diagnosed with learning disabilities when she was 8 years > old, and she continues to have problems at age 21. While working full > time in a lab where we only had laminar flow ventilation to draw away > xylene and formalin fumes, my fourth pregnancy ended in a fetal demise > at > 20 weeks. I worked full time during my next pregnancy, (1984-1985). > That > daughter has multiple minor congenital defects which include a mitral > valve prolapse, scoliosis, a concave sternum, a small area of left > temporal lobe atrophy, and a mandible deformity. She also is plagued > with > learning disabilities. Two subsequent pregnancies (1987, 1989) both > resulted in second trimester fetal demise. My high risk OB in Chicago > suggested at the time (1989) that lab chemicals may be suspect in my > case, > and when it ended he said "You're not going to try this again, are you?" > As I've heard other people state, you just never know. I'll never know. > There are other chemicals in the lab to consider as well as formalin and > xylene, tho - aniline dyes, silver nitrate, mercury, uranyl nitrate, > chloroform, toluene, to name a few. Personally, I would recommend any > pregnant woman stay out of the histology lab. I wish the NSH would > do a > study on pregnancy in the histology lab, retrospective or otherwise. > > Jacqueline M. O'Connor HT(ASCP) > Abbott Laboratories > Global Pharmaceutical Research and Development > Discovery Chemotheraputics > 847.938.4919 > > > > ------------------------------ > > Message: 18 > Date: Mon, 02 Feb 2004 09:46:45 -0700 > From: Andrea Grantham > Subject: Re: [Histonet] Re: pregnancy in lab > To: histonet@lists.utsouthwestern.edu > Message-ID: > <4.3.2.7.2.20040202092832.00cafee0@algranth.inbox.email.arizona.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > My children are 29 and 26. I wasn't working in Histology when I was > pregnant the first time. This child has a degree in Molecular Biology > and > is working in a cord blood registry lab, however despite an > exceptionally > high IQ he was diagnosed with several learning disabilities while in > elementary and jr. high and suffered from allergies to just about > everything. I know it is very controversial now but ritalin was our > salvation along with a very busy schedule. The medication didn't make > him a > zombie and it was an incredible help to him in the academic setting. > Thankfully he now does better with the allergies and he does well in > work > situations. My second child is an archeologist with a masters degree and > suffered only from being an "average" child. I was working in histology > when I was pregnant with her. I asked the pathologists and lab manager > during that time about the hazards of working around the chemicals and > they > told me not to be concerned - their only concern was how long the > maternity > leave was going to be! I took what precautions I could - it was a lot > different back then. She was born a month early but suffered no serious > effects. > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > > > ------------------------------ > > Message: 19 > Date: Mon, 02 Feb 2004 12:13:16 -0500 > From: "Richard Cartun" > Subject: [Histonet] Slide labeling pens > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass > slides. Unfortunately, our supplier no long carries them. I was > pleased to see that "Ted Pella, Inc." carries them. However, I spoke > with them this morning and they told me that they are currently NOT > available. Does anyone know a supplier for these pens? Thanks! > > Richard Cartun > > > > ------------------------------ > > Message: 20 > Date: Mon, 2 Feb 2004 11:27:08 -0600 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Re: "Need your help" > To: "'MTitford@aol.com'" , > Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain > > Our hospital has a surgical case review committee as well. We don't > to > the reqs for no specimens though. > > Hazel Horn, HT/HTL (ASCP) > Histology Supervisor > Arkansas Children's Hospital > > Phone - 501.364.4240 > Fax - 501.364.3912 > > -----Original Message----- > From: MTitford@aol.com [mailto:MTitford@aol.com] > Sent: Friday, January 30, 2004 4:29 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: "Need your help" > > > Nita Searcy asks about investigating possible unneeded surgical > procedures. > Our hospital, as part of its JCAHO accreditation (Joint Council for the > Accretation of Hospitals Organization or something close to that)has a > "Surgical Case Review Committee". It meets monthly and reviews suspect > or > unnecessary surgery's among other things. It also takes on little > projects > like reviewing appendix operations to make sure they are needed. Also as > part of our accretation processes, we require a surgical pathology slip > be > issued for each surgery that takes place, even if there is no specimen. > The > idea is the pathologist reviews the slip and if he/she thinks it is > unnecessary,or there should have been a specimen, the slip is sent on > to the > "Surgical Case Review Committee". The scrub nurse signs these off as > "none" > where the specimen should be listed. (I served on the committe for a > couple > of years. It was quite interesting. We sat in plush chairs in the > hospital > boardroom and got fed lunch....) > > Mike Titford > USA Pathology > Mobile AL USA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, you > are hereby notified that any dissemination, distribution or copying of > this communication is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. > > > > > ------------------------------ > > Message: 21 > Date: Mon, 2 Feb 2004 12:28:03 -0500 > From: Gary Gill > Subject: RE: [Histonet] Slide labeling pens > To: 'Richard Cartun' , > histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Try Google, 86 hits for redisharp plus. > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Monday, February 02, 2004 12:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide labeling pens > > > I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass > slides. Unfortunately, our supplier no long carries them. I was > pleased to > see that "Ted Pella, Inc." carries them. However, I spoke with them > this > morning and they told me that they are currently NOT available. Does > anyone > know a supplier for these pens? Thanks! > > Richard Cartun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 22 > Date: Mon, 2 Feb 2004 12:43:30 -0500 > From: "Michelle Becker" > Subject: RE: [Histonet] Slide labeling pens > To: "Richard Cartun" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Try Staples #498428 for fine black ink - $13.08 per dozen. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Monday, February 02, 2004 12:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide labeling pens > > > I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass > slides. Unfortunately, our supplier no long carries them. I was > pleased to see that "Ted Pella, Inc." carries them. However, I spoke > with them this morning and they told me that they are currently NOT > available. Does anyone know a supplier for these pens? Thanks! > > Richard Cartun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 23 > Date: Mon, 2 Feb 2004 12:56:53 -0500 > From: "Pravda, Elke" > Subject: [Histonet] Image Analysis Software > To: > Message-ID: > <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org> > Content-Type: text/plain; charset="utf-8" > > We have a post-doc in our lab that is looking for a Windows-based > software for image analysis, specifically pixel analysis, of his > confocal images. He is doing colocalization studies of his > fluorescent-labeled tissue and is looking for a program that is less > than $500 that he can load on his personal computer. We have Image-Pro > Plus in the lab, but I am not familiar with other programs (let alone > this one) and was wondering if anyone has any suggestions for us. > > Thanks so much!! > > Elke Pravda > Research Assistant > Biostructure Core Facility > Forsyth Institute > Boston, MA 02155 > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 3, Issue 2 > ************************************** From ronald <@t> klinipath.nl Mon Feb 2 13:03:17 2004 From: ronald <@t> klinipath.nl (Klinipath R.Kusters) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Justification for Tape Coverslipper Message-ID: <200402021905.i12J5YTa018215@wnet.bos.nl> Dear all, Anyone having problems with tape coming off!! Contact Mercedes Medical in Sarasota Florida (www.mercedesmedical.com) for a free sample roll of the KP-Tape. Lotts of advantages such as a much better adhesive power and price. Good luck, Ronald Kusters Netherlands -----Original Message----- >From: "Browning Deb" >Sent: 02-02-04 15:48:32 >To: "'Cheasty, Sandra'", "HistoNet (E-mail)" >Subject: RE: [Histonet] Justification for Tape Coverslipper > >Our main concern, while we do find the tape expensive, is the fact that the >tape coverslips "remove" themselves after a few years, often removing the >entire section with it, leaving us with drawers full of blank slides and >loose coverslip/tissue combo's. At this point we need to recut the original >block. The beauty of this system is that we can ship our slides within >minutes of coverslipping. > >-----Original Message----- >From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] >Sent: Sunday, February 01, 2004 4:47 PM >To: HistoNet (E-mail) >Subject: [Histonet] Justification for Tape Coverslipper > > >The staff here can hand coverslip 30 slides in 8-10 minutes >The Sakura tape coverslipper does 30 in 1.5 minutes. >With our workload this is about 2 hours a day in tech time that would be >saved. >When this tech time cost savings is weighed against the cost of the machine >and the increase in consumables (tape vs. coverslips and mounting medium) >the savings does not seem that overwhelming, even over 5 years. > >Am I missing some factor in this equation? I truly love the Sakura >coverslipper, have used it in two other labs, and even wrote a justification >for one, but that was in 1992. Has the cost of the tape gone up that much? >I remember a much bigger savings. Is my memory failing? Any input you >experts can give me would be much appreciated! (except those who blame my >failing memory...) > >Thanks > >Confidentiality Note: > >The preceding e-mail message (including any attachments) contains >information that may be confidential, protected by applicable legal >privileges, or constitute non-public information. It is intended to be >conveyed only to the designated recipient(s). If you are not an intended >recipient of this message, please notify the sender by replying to this >message and then delete it from your system. Use, dissemination, >distribution or reproduction of this message by unintended recipients is not >authorized and may be unlawful. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >This information is directed in confidence solely to the person named above >and may not otherwise be distributed, copied or disclosed. Therefore, this > [Message truncated. Tap Edit->Mark for Download to get remaining portion.] From lizchlipala <@t> premierhistology.com Mon Feb 2 12:34:48 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Image Analysis Software In-Reply-To: <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org> Message-ID: <000901c3e9bb$3fcab030$74d48a80@LIZ> Elke If he has a mac available he could use NIH image, which is free. But if you have image pro plus that would be ideal to use. He could load the software on his pc and then he would need the key, which would be on the back of the computer that has the image pro plus on it. He could get another key, since you already have a license for image pro plus, but I believe that costs around $2000.00, I would check with your image pro vendor. If there is no issue with the transfer of the key, then that might be the way to go. I have worked with both NIH image and image pro plus. NIH image works ok if you are dealing with only one color, I have found that you can use the grey scale image for this. I have not been able to work with multiple color images very well in NIH image. An example of this is counting nuclei that have been stained with Hematoxylin and then counting lets say Brdu positive cells that are DAB stained, I could not figure out a way to do this with NIH image (maybe someone with more experience could), but I was able to do this with image pro plus. Whatever program he chooses to use, it is going to take some time to figure out how it will all work. I hope this helps some and good luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pravda, Elke Sent: Monday, February 02, 2004 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Image Analysis Software We have a post-doc in our lab that is looking for a Windows-based software for image analysis, specifically pixel analysis, of his confocal images. He is doing colocalization studies of his fluorescent-labeled tissue and is looking for a program that is less than $500 that he can load on his personal computer. We have Image-Pro Plus in the lab, but I am not familiar with other programs (let alone this one) and was wondering if anyone has any suggestions for us. Thanks so much!! Elke Pravda Research Assistant Biostructure Core Facility Forsyth Institute Boston, MA 02155 From Gillian.Barlow <@t> cshs.org Mon Feb 2 13:13:22 2004 From: Gillian.Barlow <@t> cshs.org (Barlow, Gillian) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Re: pregnancy in lab Message-ID: <3CFAA0108952D111A5BF00805FA6FB0F079473FD@PEDSNTAS.csmc.edu> I've been watching these notices with interest as I have just discovered that I am pregnant. I work in a research lab, and my work includes immunohistochemistry and H&E staining by hand. The xylene baths for dewaxing slides and H&E are in a fume hood (which works, tested regularly) and we do our coverslipping in the hood also, wearing gloves of course. Is this a sufficient level of protection during pregnancy? I do this once or twice a week Many thanks Gillian Gillian M. Barlow, PhD Postdoctoral Fellow Laboratory of Julie Korenberg, PhD, MD Cedars-Sinai Medical Center Davis Bldg, Lab 2007 110 George Burns Rd Los Angeles, CA 90048 Phone: (310) 423 7650 Fax: (310) 423 0302 > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Jackie.O'Connor@abbott.com > Sent: Monday, February 2, 2004 8:08 AM > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Re: pregnancy in lab > > I've been working in histology since 1970. I didn't work in a lab during > my first two pregnancies - my oldest daughter (25) and second daughter > (24) were biology and art majors, respectively. I worked during my 3rd > pregnancy (1982) in a tox research lab with no safety measures except for > an exhaust hood for formalin trimming and xylene coverslipping. A couple > of the tox PhD's suggested I stay away from xylene during my pregnancy, so > > someone else coverslipped for me - I still did the staining. That > daughter was diagnosed with learning disabilities when she was 8 years > old, and she continues to have problems at age 21. While working full > time in a lab where we only had laminar flow ventilation to draw away > xylene and formalin fumes, my fourth pregnancy ended in a fetal demise at > 20 weeks. I worked full time during my next pregnancy, (1984-1985). That > > daughter has multiple minor congenital defects which include a mitral > valve prolapse, scoliosis, a concave sternum, a small area of left > temporal lobe atrophy, and a mandible deformity. She also is plagued with > > learning disabilities. Two subsequent pregnancies (1987, 1989) both > resulted in second trimester fetal demise. My high risk OB in Chicago > suggested at the time (1989) that lab chemicals may be suspect in my case, > > and when it ended he said "You're not going to try this again, are you?" > As I've heard other people state, you just never know. I'll never know. > There are other chemicals in the lab to consider as well as formalin and > xylene, tho - aniline dyes, silver nitrate, mercury, uranyl nitrate, > chloroform, toluene, to name a few. Personally, I would recommend any > pregnant woman stay out of the histology lab. I wish the NSH would do a > > study on pregnancy in the histology lab, retrospective or otherwise. > > Jacqueline M. O'Connor HT(ASCP) > Abbott Laboratories > Global Pharmaceutical Research and Development > Discovery Chemotheraputics > 847.938.4919 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ljb <@t> medicine.wisc.edu Mon Feb 2 11:55:07 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:31 2005 Subject: Fwd: Re: [Histonet] pregnancy in histology lab Message-ID: >>> LaCinda Burchell 02/02/04 11:42AM >>> Good Morning Stacey, 1. Yes, I have been pregnant while a Histologist. 2. No, I was not unable to do any of my duties. (Except that some brought on nausea I had never experienced before. Then again, getting out of bed made me nauseous too!) 3. My work didn't affect my pregnancy at all, but I did find that all issues of safety seemed much more poignant to me during all those days. LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> Stacey Burton 01/30/04 02:38PM >>> I am performing a pole for some inquiring employees. If anyone would like to comment - Thank you. 1. Have you ever been pregnant while working with in the histology laboratory? 2. Did your work duties have to be altered while being pregnant and to what extent? 3. Did working in the histology laboratory effect your pregnancy in any negative way? Our laboratory does perform regular ppm testing for xylene and formalin. Thank you for any comments for our pole, Stacey L. Burton, H.T. ASCP Laboratory Manager Unipath LLC San Antonio TX 210) 521-7700 staceylburton@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ljb <@t> medicine.wisc.edu Mon Feb 2 11:55:36 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:31 2005 Subject: Fwd: Re: [Histonet] pregnancy in histology lab Message-ID: >>> LaCinda Burchell 02/02/04 11:53AM >>> I did fail to mention that while my pregnancy seemed without complication my son has High Functioning Ausperger's (spelling?) Autism. A fact that has always burdened me with guilt regarding issues of fault. Cindy B LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> Stacey Burton 01/30/04 02:38PM >>> I am performing a pole for some inquiring employees. If anyone would like to comment - Thank you. 1. Have you ever been pregnant while working with in the histology laboratory? 2. Did your work duties have to be altered while being pregnant and to what extent? 3. Did working in the histology laboratory effect your pregnancy in any negative way? Our laboratory does perform regular ppm testing for xylene and formalin. Thank you for any comments for our pole, Stacey L. Burton, H.T. ASCP Laboratory Manager Unipath LLC San Antonio TX 210) 521-7700 staceylburton@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free web site building tool. Try it! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From huffpw <@t> uleth.ca Mon Feb 2 13:50:20 2004 From: huffpw <@t> uleth.ca (Phillip Huff) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Image Analysis Software In-Reply-To: <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org> References: <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org> Message-ID: <1132.166.124.185.72.1075751420.squirrel@webmail.uleth.ca> Why spend any money? NIH has a free program called ImageJ (for PCs) (or NIH Image for Macs ;-)) that has a number of macro-based add-ons for different image analysis applications. Search for either ImageJ or NIH Image (in Google of course) and you should find the program through the NIH website. It may take some time to invest in getting the correct macros (or perhaps he can write his own). Then he should share them through the NIH Image webpage. Hope this helps, Phil > We have a post-doc in our lab that is looking for a Windows-based software > for image analysis, specifically pixel analysis, of his confocal images. > He is doing colocalization studies of his fluorescent-labeled tissue and > is looking for a program that is less than $500 that he can load on his > personal computer. We have Image-Pro Plus in the lab, but I am not > familiar with other programs (let alone this one) and was wondering if > anyone has any suggestions for us. > > Thanks so much!! > > Elke Pravda > Research Assistant > Biostructure Core Facility > Forsyth Institute > Boston, MA 02155 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kerney <@t> fas.harvard.edu Mon Feb 2 14:57:03 2004 From: kerney <@t> fas.harvard.edu (Ryan Kerney) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] The right processor for ISH In-Reply-To: References: Message-ID: <5A12C292-55C2-11D8-A03A-000A95BBC3D8@fas.harvard.edu> I sent this e-mail out on Christmas Day but didn't get too many responses. Can anyone suggest the right embedding machine for small scale ISH studies. My museum is interested in getting one and we are looking around for the right price/protocol. So far we've been embedding by hand, but it would be fun to have a machine do the solvent and paraplast changes instead. thanks, Ryan On Feb 2, 2004, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Justification for Tape Coverslipper (Cheasty, Sandra) > 2. help with criostat HM 505 E (mauricio velandia) > 3. help with criostat HM 505 E (mauricio velandia) > 4. RE: combined ISH/IHC (Mikael Niku) > 5. Re: IHC validation (John Auld) > 6. BCl2 on Ventana Benchmark (Fearn Tony) > 7. Wanted dead or alive - tissue negative for connexin 43 > (Louise Carrington) > 8. RE: BCl2 on Ventana Benchmark (GUTIERREZ, JUAN) > 9. TMA Software. (LD Ridley) > 10. TMA Software. (LD Ridley) > 11. Re: TMA Software. (Helen Fedor) > 12. RE: Justification for Tape Coverslipper (Browning Deb) > 13. RE: TMA Software. (Luis Chiriboga) > 14. Fwd: [Histonet] pregnancy in histology lab (Atoska S. Gentry) > 15. RE: pregnancy in histology lab (Bonner, Janet) > 16. Antibody search (Hermina Borgerink) > 17. Re: pregnancy in lab (Jackie.O'Connor@abbott.com) > 18. Re: Re: pregnancy in lab (Andrea Grantham) > 19. Slide labeling pens (Richard Cartun) > 20. RE: Re: "Need your help" (Horn, Hazel V) > 21. RE: Slide labeling pens (Gary Gill) > 22. RE: Slide labeling pens (Michelle Becker) > 23. Image Analysis Software (Pravda, Elke) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 1 Feb 2004 13:46:40 -0800 > From: "Cheasty, Sandra" > Subject: [Histonet] Justification for Tape Coverslipper > To: "HistoNet \(E-mail\)" > Message-ID: <2E50F33F91EEDA46A77BC3B2575BB091058757@mars.llumc.edu> > Content-Type: text/plain; charset="iso-8859-1" > > The staff here can hand coverslip 30 slides in 8-10 minutes > The Sakura tape coverslipper does 30 in 1.5 minutes. > With our workload this is about 2 hours a day in tech time that would > be saved. > When this tech time cost savings is weighed against the cost of the > machine and the increase in consumables (tape vs. coverslips and > mounting medium) the savings does not seem that overwhelming, even > over 5 years. > > Am I missing some factor in this equation? I truly love the Sakura > coverslipper, have used it in two other labs, and even wrote a > justification for one, but that was in 1992. Has the cost of the tape > gone up that much? I remember a much bigger savings. Is my memory > failing? Any input you experts can give me would be much appreciated! > (except those who blame my failing memory...) > > Thanks > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains > information that may be confidential, protected by applicable legal > privileges, or constitute non-public information. It is intended to be > conveyed only to the designated recipient(s). If you are not an > intended recipient of this message, please notify the sender by > replying to this message and then delete it from your system. Use, > dissemination, distribution or reproduction of this message by > unintended recipients is not authorized and may be unlawful. > > > > > > ------------------------------ > > Message: 2 > Date: Sun, 01 Feb 2004 23:15:52 -0500 > From: "mauricio velandia" > Subject: [Histonet] help with criostat HM 505 E > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=iso-8859-1; format=flowed > > Hello to every body, excuse me for my english, it`s no very good. > > I need your help, I have a criostat Microm HM 505 E, but I haven`t > service > or user manual and when I turn on the equipment appear error E-03,and I > don?t know what the meaning this error code, if is possible and > somebody can > help me say me what is this error?, Thanks you. > > Maurice Velandia > technics > Bogot? D.C. - Colombia > > _________________________________________________________________ > Consigue aqu? las mejores y mas recientes ofertas de trabajo en Am?rica > Latina y USA: http://latam.msn.com/empleos/ > > > > > ------------------------------ > > Message: 3 > Date: Sun, 01 Feb 2004 23:21:16 -0500 > From: "mauricio velandia" > Subject: [Histonet] help with criostat HM 505 E > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=iso-8859-1; format=flowed > > Excuse me I don?t say what is my e-mail to answer about error Code > E-03 in > Hm 505 E of Microm criostat. > > My e-mail is maovel70@hotmail.com > > Thanks for your answers. > > _________________________________________________________________ > MSN Amor: busca tu ? naranja http://latam.msn.com/amor/ > > > > > ------------------------------ > > Message: 4 > Date: Mon, 2 Feb 2004 09:47:00 +0200 > From: "Mikael Niku" > Subject: RE: [Histonet] combined ISH/IHC > To: "'Tora Bardal'" , > > Message-ID: <000101c3e960$c0dcbd90$8c0fd680@ekk1116> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Tora, > > we are routinely doing combined ISH + IHC. The ISH is for genomic DNA, > so with a RNA target this might be slightly more tricky. But RNA in > tissues is surprisingly well preserved, so I think this should work. > > I spent a LOT of time trying to find a useful protocol for a double > staining. The only generally useful way I know is to use the tyramide > signal amplification system. > > This is how it goes: > > 1) Start with IHC: antigen retrieval, primary antibody, biotinylated / > HRP-conjugated secondary antibody, and then the tyramide reaction. Now > you have a covalently bound label (biotinylated tyramide, for example) > in the tissue, so you don't need to worry about antigen destruction or > stripping of bound antibodies. > > 2) Then perform the whole ISH procedure. > > 3) After you have completed the NBT/BCIP color reaction of ISH, finish > the IHC (if using biotinylated tyramide, add HRP-avidin, then DAB). > > This protocol allows you to begin with IHC (to avoid destruction of > antigens in the harsh ISH treatments) but to do the visualization step > only after ISH (to avoid false negatives caused by the DAB > precipitate). > As additional bonus, you get signal amplification for IHC, and a nicely > even DAB color intensity (nice provided that you don't need any idea of > quantitation). > > If it's the RNA that is the more sensitive target, I guess you could do > this the other way round (to use tyramide for RNA detection). > > The only drawbacks I can think of are: > > 1) A few additional incubations due to the tyramide protocol (these are > quick to do, though) > 2) The commercial tyramide reagents (which you need to use at least if > you're doing any commercial stuff, as the technology is patented) are > pretty expensive, if you're doing lots of slides. > > Please let me know if you would like to get a copy of our exact > protocol. > > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > + Mikael Niku > + University of Helsinki, Dept. Basic Veterinary Sciences > + URL: www.helsinki.fi/~mniku/ > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > > - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? > Minusta se olisi erinomainen ajatus! > (Gandhi) > > > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf >> Of Tora Bardal >> Sent: 28. tammikuuta 2004 17:16 >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] combined ISH/IHC >> >> >> Hello >> Does anyone have a working protocol they can share? >> >> I'm trying to combine in situ hybridization (RNAprobe) and >> immunohistochemistry (PCNA) on fish paraffin sections. >> So far: ISH ok, IHC doesn't work, or ISH no signal, IHC ok. >> PCNA(DAKO) >> needs AR. >> >> Before spending more time an money I would like to have some >> good advices >> on what to do when, ab simultaneous incubation? stepwise? >> crossreactions? >> blocking ? >> I'm using sheep anti-DIG-AP/BCIP/NBT (ISH) and DAKO Envision >> HRP (mouse)/DAB. >> >> Tora >> >> >> Tora Bardal >> Department of Biology, >> Norwegian University of Science and Technology (NTNU) >> Bratt?ra Research Center >> N-7491 Trondheim >> Norway + (47)73 59 09 38 / 970 25256 >> E-mail: Tora.Bardal@bio.ntnu.no fax:+ (47)73 59 63 11 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listin> fo/histonet >> > > > > > ------------------------------ > > Message: 5 > Date: Mon, 2 Feb 2004 09:35:03 +0000 > From: "John Auld" > Subject: [Histonet] Re: IHC validation > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Joyce > > In the UK there is a National EQA scheme, which also has non UK based > labs > enrolled, for IHC. There are several schemes run by the body, eg > general, > lymphoma, cytology, breast etc. EQA participation is a requirement for > accreditation in the UK and if the inspectors think we should be in > more or > different schemes will register this as a non compliance. The EQA body > also > run meetings and workshops. > > John > > Date: Fri, 30 Jan 2004 14:18:18 -0500 > From: "Joyce Cline" > Subject: [Histonet] Validation for IHC > To: > Message-ID: <002001c3e765$d180d3e0$1d2a14ac@wchsys.org> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone send their positive control tissue to another lab for > validation of their immuno staining? > The path that is over the immuno area wants me to varify the use > of my > patient controls by sending to another lab to show that these controls > are > positive by another source. > Has anyone been CAP inspected and asked if they do this? Seems he > thinks an inspector is going to ask this. > > > > > ------------------------------ > > Message: 6 > Date: Mon, 2 Feb 2004 11:37:47 -0000 > From: Fearn Tony > Subject: [Histonet] BCl2 on Ventana Benchmark > To: "Histonet (E-mail)" > Message-ID: <8C3DE9069A06D611B8950002A550C15943BD4E@BHC_MAIL02> > Content-Type: text/plain; charset="ISO-8859-1" > > We've been trying to optimise our antibodies on our Ventana Benchmark. > At > the moment we've been trying to use the DAKO antibody but it's not > been that > fantastic. Does anyone use the Ventana one, and is it any good? > Lesley > > > > > > ------------------------------ > > Message: 7 > Date: Mon, 02 Feb 2004 12:57:25 +0000 > From: "Louise Carrington" > Subject: [Histonet] Wanted dead or alive - tissue negative for > connexin 43 > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > hello , > I am trying to find a tissue negative for connexin 43 (Cx43) to > preadsorb an > antibody with and/or use as a negative staining tissue - i have been > using it to > immuno-stain fresh-frozen and frozen/fixed (ethanol, acetone, 1%, 4% > pfa) and > have ended up with nuclear staining in rat, human and bovine tissue. > it looks > 'real' ie heterogenous cell-cell staining, its not my secondary - as > ommission > of primary yeilds no background (i'm using alexafluor fluorescent > secondaries) > or my fixation as all fixation prtocols including no-fix show the same > and i've > found it in several differnet organs (whilst searching for a positive > control) > in several different species...... but the antibody is to a > phosphorylated > isoform of cx43 and my my pan connexin 43 antibody shows no nuclear > staining at > all. The phospho-cx43 has not been published yet and the company have > been > ignoring my emails so if anyone can suggest a tissue (or even cell > culture as i > get staining here as well) that has nucleate Connexin43 cells within > it that are > either > > a) abundant and easy to isolate so that i can preadsorb the antibody OR > > b) easy to recognise so that i can do immunostaining > > (or god help me ... both??) > i would be really grateful, > > i have been searching but cx 43 is a tad ubiquitous :) > > thankyou in advance > > louise > > > > Louise Carrington PhD. > Cell & Molecular Unit, > Department of Optometry & Vision Sciences, > Cardiff University, > Redwood Building, > King Edward VII Ave., > Cathays Park, > Cardiff, > Wales, > UK > > Tel 02920 875665 > email: CarringtonL@cardiff.ac.uk > > > > ------------------------------ > > Message: 8 > Date: Mon, 2 Feb 2004 08:12:18 -0600 > From: "GUTIERREZ, JUAN" > Subject: RE: [Histonet] BCl2 on Ventana Benchmark > To: "Fearn Tony" , "Histonet > (E-mail)" > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > Yes we do, and yes it is. We use CC1 standard, 28 min. primary and > A/B block. Comes out beatiful. Good luck, > > Juan > > -----Original Message----- > From: Fearn Tony [mailto:Tony.Fearn@cd.burnleyhc-tr.nwest.nhs.uk] > Sent: Mon 2/2/2004 5:37 AM > To: Histonet (E-mail) > Cc: > Subject: [Histonet] BCl2 on Ventana Benchmark > > > > We've been trying to optimise our antibodies on our Ventana > Benchmark. At > the moment we've been trying to use the DAKO antibody but it's not > been that > fantastic. Does anyone use the Ventana one, and is it any good? > Lesley > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Mon, 02 Feb 2004 14:20:37 +0000 > From: LD Ridley > Subject: [Histonet] TMA Software. > To: histonet@lists.utsouthwestern.edu > Message-ID: <1075731637.af1d4400L.D.Ridley@bham.ac.uk> > Content-Type: text/plain; charset="UTF-8" > > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are rapidly generating. I would be really grateful if people could let > me know what they are using, where it can be obtained from including > costs etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > > > ------------------------------ > > Message: 10 > Date: Mon, 02 Feb 2004 14:25:50 +0000 > From: LD Ridley > Subject: [Histonet] TMA Software. > To: histonet@lists.utsouthwestern.edu > Message-ID: <1075731950.af1d4400L.D.Ridley@bham.ac.uk> > Content-Type: text/plain; charset="UTF-8" > > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are rapidly generating. I would be really grateful if people could let > me know what they are using, where it can be obtained from including > costs etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > > > ------------------------------ > > Message: 11 > Date: Mon, 02 Feb 2004 09:45:17 -0500 > From: Helen Fedor > Subject: Re: [Histonet] TMA Software. > To: L.D.Ridley@bham.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > http://webhost5.nts.jhu.edu/~tmalab/ > this is our website. > We have our own software, Which was designed and written by our lab. > It is pretty user friendly. The data for the array design is put into > the array builder application. After the slides are stained they can be > scanned with an image analysis system, and the images are then directly > linked to the data from the scan. > you can get to our software from this page. > you can view a demo of the software and also log on as guest to view > images. > Please let me know if you need any further assistance. > > Helen > > > Helen L. Fedor B.S. > Johns Hopkins University > Brady Urological Institute > 600 N Wolfe St > Marburg Room 406 > > WARNING: E-mail sent over the Internet is not secure. Information sent > by e-mail may not remain confidential. > DISCLAIMER: This e-mail is intended only for the individual to whom it > is addressed. It may be used only in accordance with applicable laws. > If > you received this e-mail by mistake, notify the sender and destroy the > e-mail. > > Baltimore MD 21287 > email: hfedor@jhmi.edu > Phone: 410 614-1660 > Pager: 410 283-3419 > > >>>> LD Ridley 02/02/04 09:25AM >>> > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are > rapidly generating. I would be really grateful if people could let me > know what they are using, where it can be obtained from including costs > etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Mon, 2 Feb 2004 09:48:32 -0500 > From: Browning Deb > Subject: RE: [Histonet] Justification for Tape Coverslipper > To: "'Cheasty, Sandra'" , "HistoNet (E-mail)" > > Message-ID: > <3AADFB88753AD31189C100902786B91C0E277FE6@hch_nt_exchange.hhsc.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Our main concern, while we do find the tape expensive, is the fact > that the > tape coverslips "remove" themselves after a few years, often removing > the > entire section with it, leaving us with drawers full of blank slides > and > loose coverslip/tissue combo's. At this point we need to recut the > original > block. The beauty of this system is that we can ship our slides within > minutes of coverslipping. > > -----Original Message----- > From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] > Sent: Sunday, February 01, 2004 4:47 PM > To: HistoNet (E-mail) > Subject: [Histonet] Justification for Tape Coverslipper > > > The staff here can hand coverslip 30 slides in 8-10 minutes > The Sakura tape coverslipper does 30 in 1.5 minutes. > With our workload this is about 2 hours a day in tech time that would > be > saved. > When this tech time cost savings is weighed against the cost of the > machine > and the increase in consumables (tape vs. coverslips and mounting > medium) > the savings does not seem that overwhelming, even over 5 years. > > Am I missing some factor in this equation? I truly love the Sakura > coverslipper, have used it in two other labs, and even wrote a > justification > for one, but that was in 1992. Has the cost of the tape gone up that > much? > I remember a much bigger savings. Is my memory failing? Any input you > experts can give me would be much appreciated! (except those who > blame my > failing memory...) > > Thanks > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains > information that may be confidential, protected by applicable legal > privileges, or constitute non-public information. It is intended to be > conveyed only to the designated recipient(s). If you are not an > intended > recipient of this message, please notify the sender by replying to this > message and then delete it from your system. Use, dissemination, > distribution or reproduction of this message by unintended recipients > is not > authorized and may be unlawful. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This information is directed in confidence solely to the person named > above > and may not otherwise be distributed, copied or disclosed. Therefore, > this > information should be considered strictly confidential. If you have > received this email in error, please notify the sender immediately via > a > return email for further direction. Thank you for your assistance. > > > > > > ------------------------------ > > Message: 13 > Date: Mon, 02 Feb 2004 10:06:01 -0500 > From: Luis Chiriboga > Subject: RE: [Histonet] TMA Software. > To: LD Ridley , > histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=utf-8 > > Chih Long Lui etal American journal of Pathology V161(5)Nov02 1557-1565 > Just started using & so far not to bad. software is based on gene > microarray analysis so it's pretty complimentary. can get more info > from their website. It's freeware. > http://genome-www.stanford.edu/TMA/index.shtml > > Luis > > > -------------------------------------- > Luis Chiriboga Ph.D., HT (ASCP) QIHC > New York University School Of Medicine > NYU Cancer Institute and > Bellevue Hospital Center > Department Of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > (212) 562-4667. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LD > Ridley > Sent: Monday, February 02, 2004 9:21 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TMA Software. > > > This is just an enquiry to all those out there that construct and > analyse Tissue Microarrays in the lab. We are looking into the various > analysis software packages availible to try and organise our data we > are rapidly generating. I would be really grateful if people could let > me know what they are using, where it can be obtained from including > costs etc and any other opinions you have of it. > Many thanks in advance for your help > Lee > Lee Ridley > Research Associate > > L.D.Ridley@bham.ac.uk > Cancer Research UK Institute for Cancer Studies > University of Birmingham > Vincent Drive > Edgbaston > Birmingham > B15 2TT > UK > > +44 (0)121 414 4472 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Mon, 02 Feb 2004 08:50:59 -0600 > From: "Atoska S. Gentry" > Subject: Fwd: [Histonet] pregnancy in histology lab > To: Histonet > Message-ID: > <6.0.1.1.0.20040202084903.025ba2a0@mailhost.vetmed.auburn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > >> Date: Fri, 30 Jan 2004 12:38:05 -0800 (PST) >> From: Stacey Burton >> To: histonet@pathology.swmed.edu >> X-Scan-Signature: 6696ac9d93c496fe8014a1b98c524a58 >> X-Content-Filtered-By: Mailman/MimeDel 2.1.3 >> Cc: >> X-BeenThere: histonet@lists.utsouthwestern.edu >> X-Mailman-Version: 2.1.3 >> List-Id: For the exchange of information pertaining to >> histotechnology and >> related fields >> List-Unsubscribe: >> , >> >> >> List-Archive: >> List-Post: >> List-Help: >> >> List-Subscribe: >> , >> >> >> Sender: histonet-bounces@lists.utsouthwestern.edu >> X-Scan-Signature: 976d3788468f6168a965852c1e2d4d6f >> X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >> Subject: [Histonet] pregnancy in histology lab >> X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >> swlx162.swmed.edu >> X-Spam-Level: >> X-Spam-Status: No, hits=0.2 required=6.5 >> tests=RCVD_IN_NJABL,RCVD_IN_SORBS >> autolearn=no version=2.63 >> X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >> X-SA-Exim-Scanned: Yes >> >> I am performing a pole for some inquiring employees. If anyone would >> like >> to comment - Thank you. >> >> 1. Have you ever been pregnant while working with in the histology >> laboratory? Yes, twice. >> >> 2. Did your work duties have to be altered while being pregnant and >> to >> what extent? No. >> >> 3. Did working in the histology laboratory effect your pregnancy in >> any >> negative way? No. > >> >> Our laboratory does perform regular ppm testing for xylene and >> formalin. >> >> Thank you for any comments for our pole, >> >> Stacey L. Burton, H.T. ASCP >> Laboratory Manager >> Unipath LLC >> San Antonio TX >> 210) 521-7700 >> staceylburton@yahoo.com >> >> >> >> >> >> --------------------------------- >> Do you Yahoo!? >> Yahoo! SiteBuilder - Free web site building tool. Try it! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > > > ------------------------------ > > Message: 15 > Date: Mon, 2 Feb 2004 10:28:39 -0500 > From: "Bonner, Janet" > Subject: RE: [Histonet] pregnancy in histology lab > To: "'Gudrun Lang'" , Histonetliste > , Stacey Burton > > Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F4B@fh2k093.fhmis.net> > Content-Type: text/plain; charset="iso-8859-1" > > I think that if anything develops later on in your children, you'll > always > wonder what role Histology may have played. I worked in a lab in > 1980-81 > when I was pregnant and my son seemed fine - until last week. An Echo > cardiogram showed a missing tricuspid valve and the other two were > fused! > Of course now he's 22 and been through things like strept throat and > ear > aches and God only knows what other flus, genetic abnormalities never > discovered before ECHOs, etc. Enjoy life while we have it together, > but > ALWAYS take precautions no matter what you are doing- > (My older collegues suffered as a result of choloroform which used to > be > widely used in Histology but is now restricted/prohibited.) > > -----Original Message----- > From: Gudrun Lang [mailto:gudrun.lang@aon.at] > Sent: Friday, January 30, 2004 5:27 PM > To: Histonetliste; Stacey Burton > Subject: Re: [Histonet] pregnancy in histology lab > > > My children are 8 and 11 years old, and until now they are obviosly > healthy. > During pregnancy I was not allowed to be in rooms with formalin, xylol > or > any other probably cancerogen reagens. I was not allowed to handle with > nativ probes, like making cryo sections or grossing. > My duties were cutting in a seperate room and doing administrative > jobs. > I had a coworker, who had her children in the 1960s. In this time, > there > were no fumehoods and xylol-wet slides were dried near the radiator. > Using > gloves was not really appriciated. But yet her girls are healthy and > have > also healthy children. > But something irritated me, when she told about the good old times. Her > elder colleages didn't reach a really high age and often had some > cancer > disease?! > hope this helps > > Gudrun Lang > Linz, Austria > ----- Original Message ----- > From: "Stacey Burton" > To: > Sent: Friday, January 30, 2004 9:38 PM > Subject: [Histonet] pregnancy in histology lab > > >> I am performing a pole for some inquiring employees. If anyone would >> like > to comment - Thank you. >> >> 1. Have you ever been pregnant while working with in the histology > laboratory? >> >> 2. Did your work duties have to be altered while being pregnant and >> to > what extent? >> >> 3. Did working in the histology laboratory effect your pregnancy in >> any > negative way? >> >> Our laboratory does perform regular ppm testing for xylene and >> formalin. >> >> Thank you for any comments for our pole, >> >> Stacey L. Burton, H.T. ASCP >> Laboratory Manager >> Unipath LLC >> San Antonio TX >> 210) 521-7700 >> staceylburton@yahoo.com >> >> >> >> >> >> --------------------------------- >> Do you Yahoo!? >> Yahoo! SiteBuilder - Free web site building tool. Try it! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be privileged and/or > confidential and protected from disclosure. If the reader of this > message > is not the intended recipient or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is > strictly > prohibited. If you have received this communication in error, please > notify > the sender immediately by replying to this message and deleting the > material > from any computer. > > > > ------------------------------ > > Message: 16 > Date: Mon, 2 Feb 2004 10:29:29 -0500 > From: "Hermina Borgerink" > Subject: [Histonet] Antibody search > To: "histonet" > Message-ID: > > <9AEEF1FB6254224AA355ED285F84916503F8EA13@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="US-ASCII" > > Hi all, > One of our research investigators is looking for two antibodies: (1) > Steroid sulfatase and (2) 17 beta hydroxysteroid dehydrogenase. I did > an online search but came up with nothing. He was also interested in > knowing if any of you have successfully used Cytochrome P450 Aromatase > from Serotec on FFPE tissues. I would greatly appreciate any input you > might be able to give me. > Hermina > > Hermina M. Borgerink, BA, HTL(ASCP)QIHC > Wake Forest University Health Sciences > Department of Pathology > Medical Center Blvd. > Winston-Salem, NC 27157 > Tel. (336) 716-1538 > Fax (336) 716-1515 > e-mail hborgeri@wfubmc.edu > > > > > ------------------------------ > > Message: 17 > Date: Mon, 2 Feb 2004 10:08:36 -0600 > From: Jackie.O'Connor@abbott.com > Subject: [Histonet] Re: pregnancy in lab > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > ON86256E2E.0056AC80@northamerica.intra.abbott.com> > > Content-Type: text/plain; charset="us-ascii" > > I've been working in histology since 1970. I didn't work in a lab > during > my first two pregnancies - my oldest daughter (25) and second daughter > (24) were biology and art majors, respectively. I worked during my 3rd > pregnancy (1982) in a tox research lab with no safety measures except > for > an exhaust hood for formalin trimming and xylene coverslipping. A > couple > of the tox PhD's suggested I stay away from xylene during my > pregnancy, so > someone else coverslipped for me - I still did the staining. That > daughter was diagnosed with learning disabilities when she was 8 years > old, and she continues to have problems at age 21. While working full > time in a lab where we only had laminar flow ventilation to draw away > xylene and formalin fumes, my fourth pregnancy ended in a fetal demise > at > 20 weeks. I worked full time during my next pregnancy, (1984-1985). > That > daughter has multiple minor congenital defects which include a mitral > valve prolapse, scoliosis, a concave sternum, a small area of left > temporal lobe atrophy, and a mandible deformity. She also is plagued > with > learning disabilities. Two subsequent pregnancies (1987, 1989) both > resulted in second trimester fetal demise. My high risk OB in Chicago > suggested at the time (1989) that lab chemicals may be suspect in my > case, > and when it ended he said "You're not going to try this again, are > you?" > As I've heard other people state, you just never know. I'll never > know. > There are other chemicals in the lab to consider as well as formalin > and > xylene, tho - aniline dyes, silver nitrate, mercury, uranyl nitrate, > chloroform, toluene, to name a few. Personally, I would recommend any > pregnant woman stay out of the histology lab. I wish the NSH would > do a > study on pregnancy in the histology lab, retrospective or otherwise. > > Jacqueline M. O'Connor HT(ASCP) > Abbott Laboratories > Global Pharmaceutical Research and Development > Discovery Chemotheraputics > 847.938.4919 > > > > ------------------------------ > > Message: 18 > Date: Mon, 02 Feb 2004 09:46:45 -0700 > From: Andrea Grantham > Subject: Re: [Histonet] Re: pregnancy in lab > To: histonet@lists.utsouthwestern.edu > Message-ID: > <4.3.2.7.2.20040202092832.00cafee0@algranth.inbox.email.arizona.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > My children are 29 and 26. I wasn't working in Histology when I was > pregnant the first time. This child has a degree in Molecular Biology > and > is working in a cord blood registry lab, however despite an > exceptionally > high IQ he was diagnosed with several learning disabilities while in > elementary and jr. high and suffered from allergies to just about > everything. I know it is very controversial now but ritalin was our > salvation along with a very busy schedule. The medication didn't make > him a > zombie and it was an incredible help to him in the academic setting. > Thankfully he now does better with the allergies and he does well in > work > situations. My second child is an archeologist with a masters degree > and > suffered only from being an "average" child. I was working in histology > when I was pregnant with her. I asked the pathologists and lab manager > during that time about the hazards of working around the chemicals and > they > told me not to be concerned - their only concern was how long the > maternity > leave was going to be! I took what precautions I could - it was a lot > different back then. She was born a month early but suffered no serious > effects. > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) > : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > > > ------------------------------ > > Message: 19 > Date: Mon, 02 Feb 2004 12:13:16 -0500 > From: "Richard Cartun" > Subject: [Histonet] Slide labeling pens > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass > slides. Unfortunately, our supplier no long carries them. I was > pleased to see that "Ted Pella, Inc." carries them. However, I spoke > with them this morning and they told me that they are currently NOT > available. Does anyone know a supplier for these pens? Thanks! > > Richard Cartun > > > > ------------------------------ > > Message: 20 > Date: Mon, 2 Feb 2004 11:27:08 -0600 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Re: "Need your help" > To: "'MTitford@aol.com'" , > Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain > > Our hospital has a surgical case review committee as well. We don't > to > the reqs for no specimens though. > > Hazel Horn, HT/HTL (ASCP) > Histology Supervisor > Arkansas Children's Hospital > > Phone - 501.364.4240 > Fax - 501.364.3912 > > -----Original Message----- > From: MTitford@aol.com [mailto:MTitford@aol.com] > Sent: Friday, January 30, 2004 4:29 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: "Need your help" > > > Nita Searcy asks about investigating possible unneeded surgical > procedures. > Our hospital, as part of its JCAHO accreditation (Joint Council for the > Accretation of Hospitals Organization or something close to that)has a > "Surgical Case Review Committee". It meets monthly and reviews suspect > or > unnecessary surgery's among other things. It also takes on little > projects > like reviewing appendix operations to make sure they are needed. Also > as > part of our accretation processes, we require a surgical pathology > slip be > issued for each surgery that takes place, even if there is no > specimen. The > idea is the pathologist reviews the slip and if he/she thinks it is > unnecessary,or there should have been a specimen, the slip is sent on > to the > "Surgical Case Review Committee". The scrub nurse signs these off as > "none" > where the specimen should be listed. (I served on the committe for a > couple > of years. It was quite interesting. We sat in plush chairs in the > hospital > boardroom and got fed lunch....) > > Mike Titford > USA Pathology > Mobile AL USA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, you > are hereby notified that any dissemination, distribution or copying of > this communication is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to > the message and deleting it from your computer. Thank you. > > > > > ------------------------------ > > Message: 21 > Date: Mon, 2 Feb 2004 12:28:03 -0500 > From: Gary Gill > Subject: RE: [Histonet] Slide labeling pens > To: 'Richard Cartun' , > histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Try Google, 86 hits for redisharp plus. > > -----Original Message----- > From: Richard Cartun [mailto:Rcartun@harthosp.org] > Sent: Monday, February 02, 2004 12:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide labeling pens > > > I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass > slides. Unfortunately, our supplier no long carries them. I was > pleased to > see that "Ted Pella, Inc." carries them. However, I spoke with them > this > morning and they told me that they are currently NOT available. Does > anyone > know a supplier for these pens? Thanks! > > Richard Cartun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 22 > Date: Mon, 2 Feb 2004 12:43:30 -0500 > From: "Michelle Becker" > Subject: RE: [Histonet] Slide labeling pens > To: "Richard Cartun" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Try Staples #498428 for fine black ink - $13.08 per dozen. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Monday, February 02, 2004 12:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide labeling pens > > > I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass > slides. Unfortunately, our supplier no long carries them. I was > pleased to see that "Ted Pella, Inc." carries them. However, I spoke > with them this morning and they told me that they are currently NOT > available. Does anyone know a supplier for these pens? Thanks! > > Richard Cartun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 23 > Date: Mon, 2 Feb 2004 12:56:53 -0500 > From: "Pravda, Elke" > Subject: [Histonet] Image Analysis Software > To: > Message-ID: > <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org> > Content-Type: text/plain; charset="utf-8" > > We have a post-doc in our lab that is looking for a Windows-based > software for image analysis, specifically pixel analysis, of his > confocal images. He is doing colocalization studies of his > fluorescent-labeled tissue and is looking for a program that is less > than $500 that he can load on his personal computer. We have Image-Pro > Plus in the lab, but I am not familiar with other programs (let alone > this one) and was wondering if anyone has any suggestions for us. > > Thanks so much!! > > Elke Pravda > Research Assistant > Biostructure Core Facility > Forsyth Institute > Boston, MA 02155 > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 3, Issue 2 > ************************************** > > Ryan Kerney Museum of Comparative Zoology Harvard University 26 Oxford St. Cambridge, MA 02138 Lab: 617-496-4632 Office: 617-496-4065 From kennedya <@t> email.cs.nsw.gov.au Mon Feb 2 15:23:00 2004 From: kennedya <@t> email.cs.nsw.gov.au (Andrew Kennedy) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] KP Tape Message-ID: <004301c3e9d2$be244d80$94e04c98@LAB0216.cs.nsw.gov.au> KP Tape!? - been there, done that - it still comes off the same as the other tape, taking the sections with it!! Most of our slides lose the tape 'slips in around about two years. Far better to go with a glass coverslipper. They are a bit slower, but still faster than hand coverslipping...and glass is for good!!! Andrew Kennedy Senior Science Officer in charge - Histopathology. Department of Anatomical Pathology Concord Repatriation General Hospital Hospital Road Concord, NSW, Australia 2139 Phone: +612 9767 6115 Fax: +612 9767 8427 "Noli illegitimi carborundum" ------------------------------------------------------------------------------------------------------------ This message is intended for the addressee(s) named and may contain confidential information. If you are not an intended recipient, please delete this email and notify the sender. Views expressed in this message are those of the individual sender and are not necessarily the views of Central Sydney Area Health Service. ------------------------------------------------------------------------------------------------------------ From garygill <@t> dcla.com Mon Feb 2 15:26:19 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] KP Tape Message-ID: Tape is a pane in the glass. -----Original Message----- From: Andrew Kennedy [mailto:kennedya@email.cs.nsw.gov.au] Sent: Monday, February 02, 2004 4:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Tape KP Tape!? - been there, done that - it still comes off the same as the other tape, taking the sections with it!! Most of our slides lose the tape 'slips in around about two years. Far better to go with a glass coverslipper. They are a bit slower, but still faster than hand coverslipping...and glass is for good!!! Andrew Kennedy Senior Science Officer in charge - Histopathology. Department of Anatomical Pathology Concord Repatriation General Hospital Hospital Road Concord, NSW, Australia 2139 Phone: +612 9767 6115 Fax: +612 9767 8427 "Noli illegitimi carborundum" ---------------------------------------------------------------------------- -------------------------------- This message is intended for the addressee(s) named and may contain confidential information. If you are not an intended recipient, please delete this email and notify the sender. Views expressed in this message are those of the individual sender and are not necessarily the views of Central Sydney Area Health Service. ---------------------------------------------------------------------------- -------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DELONG_CYNTHIA_A <@t> LILLY.COM Mon Feb 2 17:08:38 2004 From: DELONG_CYNTHIA_A <@t> LILLY.COM (Cynthia A Delong) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] staining rack for 18 x18 coverslips Message-ID: Does anyone know of a company that makes a (staining rack) for 18 x 18 coverslips. I am staining sections on coverslips that need to be stained and right now I am doing each one individually. I would like to be able to bulk stain. I know what everyone is thinking (Why doesn't she just put them on slides) I cannot place these sections on slides. These sections are having other things being done to them that will not allow them to be on slides. Thank you for all of your help. Cindy Cynthia A DeLong LRL - Corporate Center DC0533 - 48B/3042 355 E Merrill St Indianapolis, IN 46225 phone: 317-276-7635 fax: 317-277-6146 From ploykasek <@t> phenopath.com Mon Feb 2 17:09:11 2004 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Antibody validation Message-ID: Just a few thoughts on the whole antibody validation format. I won't claim to have figured the complete meaning behind all the regulations, but here goes. I think it is incumbent upon each lab to validate the antibodies they are using in their lab on their specimens with their reagents. I do mean more than just a titer and pretreatment work-up. The antibody sensitivity, specificity, accuracy and precision must be addressed. This will entail collecting journal articles, staining a set of expected positive & expected negative cases. Then you must analyze what this data means for this antibody. This is fairly easy with some antibodies, and difficult with others. We then have a lay out that we "plot" this information into, and expound on these topics of the antibody. I do realize this is a lot of work, and not done overnight. Just some things to think about. Thanks Patti Loykasek PhenoPath Laboratories Seattle, EA From ploykasek <@t> phenopath.com Mon Feb 2 17:49:20 2004 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Tape coverslip Message-ID: We have not had trouble with the tape coming off slides. In use here for almost 6 years. Perhaps it has to do with climate, amount of xylene initially placed on slide, or maybe how you held your mouth that day! Patti Loykasek PhenoPath Labs Seattle, WA From Jason.PALMER <@t> svhm.org.au Tue Feb 3 00:24:35 2004 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Zymed monoclonal Ab to B-galactosidase (ZBG1 clone) Message-ID: Hi Histonetters. I'm trying to get the above antibody working with immunohistochemistry, but without any luck. I have tried FFPE tissues, with different pretreatment / retrieval steps, and snap frozen tissue, cut then fixed in acetone or paraformaldehyde, all to no avail. I'm using the DAKO ARK kit since I'm using mouse antibody on mouse (transgenic) tissue. Has anybody had success of any sort with this antibody? Any responses much appreciated. Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: palmerj@svhm.org.au -------------- next part -------------- Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. From kwuny <@t> email.cs.nsw.gov.au Tue Feb 3 00:56:17 2004 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Slide labeling pens Message-ID: <01C3EA7F.065FBE70.kwuny@email.cs.nsw.gov.au> Another fantastic pen for labelling glass slides would be Mitsubishi uniPIN fine line. It is a pigment ink pen. Once dried, waterter and fade proof (by most solvents including xylene). Good stationary stores would have them. Young Kwun -----Original Message----- From: Gary Gill [SMTP:garygill@dcla.com] Sent: Tuesday, 3 February 2004 04:28 To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labeling pens Try Google, 86 hits for redisharp plus. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Monday, February 02, 2004 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling pens I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass slides. Unfortunately, our supplier no long carries them. I was pleased to see that "Ted Pella, Inc." carries them. However, I spoke with them this morning and they told me that they are currently NOT available. Does anyone know a supplier for these pens? Thanks! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Central Sydney Area Health Service." From j.p.g.ooms <@t> zonnet.nl Tue Feb 3 03:45:32 2004 From: j.p.g.ooms <@t> zonnet.nl (Hans Ooms) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] cowdrybodies Message-ID: <002301c3ea3a$78c2a210$8867a63e@st2c8pdli7fy3i> Hello folkes, Does anyone know if there are antibodies against Cowdry-bodies? Thanks Hans From juan.gutierrez <@t> christushealth.org Tue Feb 3 07:09:54 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] staining rack for 18 x18 coverslips Message-ID: Thermo (Shandon) has one that will hold 22mm coverslips, but I'm afraid that's as small as they come. Can you switch to bigger coverslips? I use to do the muscle enzyme reactions on 22 and 24mm coverslips using the two types of racks that Thermo sells with great success. Good luck on your search. Juan p.s. If you are interested in the Thermo racks their order #s are: 116 for the 5 slide holder. 114 for the 30 slide holder. You have to call them for pricing. 1-800-547-7429 -----Original Message----- From: Cynthia A Delong [mailto:DELONG_CYNTHIA_A@LILLY.COM] Sent: Mon 2/2/2004 5:08 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] staining rack for 18 x18 coverslips Does anyone know of a company that makes a (staining rack) for 18 x 18 coverslips. I am staining sections on coverslips that need to be stained and right now I am doing each one individually. I would like to be able to bulk stain. I know what everyone is thinking (Why doesn't she just put them on slides) I cannot place these sections on slides. These sections are having other things being done to them that will not allow them to be on slides. Thank you for all of your help. Cindy Cynthia A DeLong LRL - Corporate Center DC0533 - 48B/3042 355 E Merrill St Indianapolis, IN 46225 phone: 317-276-7635 fax: 317-277-6146 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eshields <@t> bhset.org Tue Feb 3 07:20:39 2004 From: eshields <@t> bhset.org (E Sharon Shields) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Help from Durham NC Hisotnetters Message-ID: My husband is going to Duke March 1st for an evaluation for a possible lung transplant. I need a safe, clean, cheap place for us to stay for the first week of March, any suggestions? Do you know anything about the InnKeepers Durham West? I found the place on the internet. Any suggestion would be greatly appreciated. Sharon Baptist Hospital of E TN 865 549-4351 8:00 to 4:30 M - F From Jacquie.Mack <@t> CLS.ab.ca Tue Feb 3 07:58:21 2004 From: Jacquie.Mack <@t> CLS.ab.ca (Jacquie.Mack@CLS.ab.ca) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] unsubscribe Message-ID: <30C050525B881C4AAFF41E6D16543E6803488A15@mail3.cls.ab.ca> unsubscribe Jacqueline Mack Tech III , Anatomic Pathology Foothills Medical Center Calgary Laboratory Services ( (403)-944-4162 fax: (403)-270-4093 pager: 212-8223 # 0540 * Ja cquie.Mack@CLS.ab.ca Laboratory Medicine Enhancing Your Health Care From MAUGER <@t> email.chop.edu Tue Feb 3 08:08:17 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] staining rack for 18 x18 coverslips Message-ID: To Cindy, You can glue your coverslips onto regular glass slides with mounting medium. Put a drop on the slide, and place specimen up on the slide. Let it dry well. this works great! Jo >>> "GUTIERREZ, JUAN" 02/03/04 08:09AM >>> Thermo (Shandon) has one that will hold 22mm coverslips, but I'm afraid that's as small as they come. Can you switch to bigger coverslips? I use to do the muscle enzyme reactions on 22 and 24mm coverslips using the two types of racks that Thermo sells with great success. Good luck on your search. Juan p.s. If you are interested in the Thermo racks their order #s are: 116 for the 5 slide holder. 114 for the 30 slide holder. You have to call them for pricing. 1-800-547-7429 -----Original Message----- From: Cynthia A Delong [mailto:DELONG_CYNTHIA_A@LILLY.COM] Sent: Mon 2/2/2004 5:08 PM To: Histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] staining rack for 18 x18 coverslips Does anyone know of a company that makes a (staining rack) for 18 x 18 coverslips. I am staining sections on coverslips that need to be stained and right now I am doing each one individually. I would like to be able to bulk stain. I know what everyone is thinking (Why doesn't she just put them on slides) I cannot place these sections on slides. These sections are having other things being done to them that will not allow them to be on slides. Thank you for all of your help. Cindy Cynthia A DeLong LRL - Corporate Center DC0533 - 48B/3042 355 E Merrill St Indianapolis, IN 46225 phone: 317-276-7635 fax: 317-277-6146 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDittus787 <@t> aol.com Tue Feb 3 08:19:58 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Antibody validation Message-ID: <44408969.3382419F.0A1F969F@aol.com> patti: i think your ideas are very good and sound, can you share your format with us? Dana From Ronnie_Houston <@t> bshsi.com Tue Feb 3 09:40:02 2004 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] liability issues Message-ID: <530361BF03351B4CAE5270A05D3037B5FE065F@bsrexms01.BSHSIR.COM> Is anyone aware of there are any legal liability issues when providing a technical component service only, both within and outwith one's native state? thanks Ronnie Houston ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From bingham <@t> cvm.msstate.edu Tue Feb 3 11:29:37 2004 From: bingham <@t> cvm.msstate.edu (Willie Bingham) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] How to get on the daily e-mail list Message-ID: Hello, To whom it may concern, I subscribed to histonet on yesterday evening. I think I failed to sign up for the daily e-mail FAQs. How do I sign up for this service? Thanks, WB From padunnje <@t> iupui.edu Tue Feb 3 14:44:54 2004 From: padunnje <@t> iupui.edu (Dunn-Jena, Patsy A) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] question/help-Marking ink Message-ID: Normally do Bone work in the laboratory, but recently did some skin for an investigator and wished afterwards that we had marked the cut surface. Can anybody please give some suggestions on where to locate marking ink? I would really appreciate it. Patsy Dunn-Jena, RVT, LAT, HT (ASCP) Indiana University School of Dentistry Mineralized Tissue and Histology Research Laboratory 1121 West Michigan Street, Room 238 Indianapolis, IN 46202 (317) 274-0544 padunnje@iupui.edu From scoop <@t> mail.nih.gov Tue Feb 3 15:00:15 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] marking ink Message-ID: Dear Histonetters, I'm also looking for marking ink, something I can use to mark the orientation of a mouse spinal cord (dissected out of the spine) to tell which side is dorsal or ventral to orient myself for embedding. Does anyone know of something that would work for this? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From travers.3 <@t> osu.edu Tue Feb 3 17:34:05 2004 From: travers.3 <@t> osu.edu (Susan Travers) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] soluble guanylyl cyclase aby for ihc Message-ID: <74FB5F21-56A1-11D8-B85E-0030655671F2@osu.edu> Does anyone know of a commercially available source of soluble guanylyl cyclase that will work in immunohistochemistry? We are particularly interested in using it on brain tissue. Thanks for any tips! Susan Travers From meyenhmf <@t> umdnj.edu Tue Feb 3 15:11:33 2004 From: meyenhmf <@t> umdnj.edu (meyenhmf@umdnj.edu) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] Cell Prep (TM) Antigen retrieval Message-ID: <1075842693.40200e85b1306@webmail.umdnj.edu> Hello all. Is there something comparable to "Cell Prep" (TM) that can be prepared in the lab? Regards, the "cheap" guy. -- From lizchlipala <@t> premierhistology.com Tue Feb 3 15:25:43 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] marking ink In-Reply-To: Message-ID: <000001c3ea9c$4b55ae50$74d48a80@LIZ> You can use Indian ink or any colored tattoo ink. I believe TBS sells a colored marking system, they are called tissue marking dyes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Cooperman Sent: Tuesday, February 03, 2004 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking ink Dear Histonetters, I'm also looking for marking ink, something I can use to mark the orientation of a mouse spinal cord (dissected out of the spine) to tell which side is dorsal or ventral to orient myself for embedding. Does anyone know of something that would work for this? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histolog <@t> fcv.unl.edu.ar Tue Feb 3 15:50:05 2004 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] erbB-2 protocol Message-ID: <000001c3ea9f$b010da60$5a0cd2aa@unl.edu.ar> Does any one have a good protocol for an immuno stain using the Dako Rabbit Anti-Human c-erbB-2 Oncoprotein antibody. If so, which antigen retrieval solution / protocol worked best for you and which the best positive control? Thankyou in advance? Hugo ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From cfavara <@t> niaid.nih.gov Tue Feb 3 15:54:54 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:31 2005 Subject: [Histonet] marking ink Message-ID: Sharon, I have tried numerous inking dyes for mouse brain and spinal cord and have found that they obscure the stain we use here to a significant extent. I have found that the best thing to do it to get a very good pair of magnifying glasses and familiarize yourself with the anatomy. I also place the spinal cord on tissue/lens paper with a specified side down. Hope this helps. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Cooperman, Sharon (NIH/NICHD) Sent: Tuesday, February 03, 2004 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking ink Dear Histonetters, I'm also looking for marking ink, something I can use to mark the orientation of a mouse spinal cord (dissected out of the spine) to tell which side is dorsal or ventral to orient myself for embedding. Does anyone know of something that would work for this? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Feb 3 16:04:10 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Hazards to pregnant histotechs Message-ID: for those of you who were discussing concerns about working in the lab while pregnant, I thought I would pass along this URL that I just stumbled upon at that Anatech website entitled XYLENE CONFIRMED AS A REPRODUCTIVE HAZARD you can find it here http://www.anatechltdusa.com/Innovators/5_InnXylHaz.html Vinnie From mcauliff <@t> umdnj.edu Tue Feb 3 19:26:41 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] liability issues In-Reply-To: <530361BF03351B4CAE5270A05D3037B5FE065F@bsrexms01.BSHSIR.COM> References: <530361BF03351B4CAE5270A05D3037B5FE065F@bsrexms01.BSHSIR.COM> Message-ID: <40204A51.70002@umdnj.edu> I think you should ask a lawyer who 1.practices in the state you are concerned about. 2. Is knowledgable about the specific area. Geoff Houston, Ronnie wrote: >Is anyone aware of there are any legal liability issues when providing a >technical component service only, both within and outwith one's native >state? > >thanks > >Ronnie Houston > >________________________________________________________________________________________________________________________________ >________________________________________________________________________________________________________________________________ > >The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. >It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. >In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information >contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should >this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, >and permanently delete the original e-mail, attachment(s), and any copies. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From dellav <@t> musc.edu Tue Feb 3 16:46:07 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] question/help-Marking ink Message-ID: Fisher Scientific distributes Tissue Marking dyes from TBS. Dyes may be purchased in a set of five different colors or may be purchased indvidiually. You can reach Fisher at 1-800-640-0640. Similar dye products are available from other sources. One is called Davidson Marking System which is another selection of different colored dyes. Someone else on fhis forum will know where to buy. Lastly, some labs have purchased and successfully used tatoo dyes which can be purchased from various sources on the internet. do a google search. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Dunn-Jena, Patsy A" 02/03/04 03:44PM >>> Normally do Bone work in the laboratory, but recently did some skin for an investigator and wished afterwards that we had marked the cut surface. Can anybody please give some suggestions on where to locate marking ink? I would really appreciate it. Patsy Dunn-Jena, RVT, LAT, HT (ASCP) Indiana University School of Dentistry Mineralized Tissue and Histology Research Laboratory 1121 West Michigan Street, Room 238 Indianapolis, IN 46202 (317) 274-0544 padunnje@iupui.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael_lafriniere <@t> memorial.org Tue Feb 3 20:55:51 2004 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] (no subject) Message-ID: Wayne, and all who were interested in the two specimens in one container, separation of and appropriate billing practice. This is the response from CAP Question asked: At any time can one split up two specimens received in one container and bill two separate CPT codes? Answer Rec'd from CAP: "Only if the specimens are separately identifiable." Regards, Michael LaFriniere PA, HT(ASCP) From christl <@t> chempath.uct.ac.za Tue Feb 3 22:48:06 2004 From: christl <@t> chempath.uct.ac.za (Christl Honiball) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] 4-HNE Antibody Message-ID: <40207986.AD8CB858@chempath.uct.ac.za> Hello Histonetters Is there anyone who has used the Chemicon Goat Anti-4-Hydroxynonenal (Cat.#AB5605) antibody on IHC? I would really appreciate any info that might be available. Regards, Christl Honiball From vandries <@t> vub.ac.be Wed Feb 4 04:00:29 2004 From: vandries <@t> vub.ac.be (Veronique Andriessen) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] staining for stellate cells In-Reply-To: <1674D52D81376743AE9AC83C6A50F0B72C6998@hilda2.inexsys.org> Message-ID: Hi Michele, Try staining for Desmin and GFAP. We have exellent results using Sigma monoclonal antibodies in combination with the DAKO ARK kit. Best regards, Veronique Andriessen Cell biology and Histology Free University of Brussels Belgium -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michele Ellender Sent: woensdag 28 januari 2004 12:22 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] staining for stellate cells Hello all, does anyone have a staining method to stain for stellate cells in mouse liver sections? Thanks, Michele Dr Michele Ellender Radiation Effects Department, NRPB, Chilton, Didcot, Oxon. OX11 0RQ. UK michele.ellender@nrpb.org This e-mail transmission is strictly confidential and intended solely for the person or organisation to whom it is addressed. It may contain privileged and confidential information and if you are not the intended recipient, please do not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, please notify the sender as soon as possible and delete the message. Please note that NRPB monitors incoming and outgoing e-mail for compliance with its Acceptable Use Policy. This will include scanning incoming e-mails to detect viruses and key-words and may in some circumstances result in the manual monitoring of the content of messages. National Radiological Protection Board E-mail: nrpb@nrpb.org Web site: www.nrpb.org -------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cycling <@t> another.com Wed Feb 4 08:10:24 2004 From: cycling <@t> another.com (cycling@another.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] The right processor for ISH Message-ID: <5350024.1075903824453.JavaMail.root@172.16.100.50> Maybe we are talking Processing machine as the subject line says,rather than embedding machine, embedding being the point of tissue going into wax and solidifying. Thermo Citadel brand processor is/was simple and uncomplicated. Dave Ed Christie Hosp, Manchester UK -----Original Message----- >From : Ryan Kerney To : histonet@lists.utsouthwestern.edu Date : 02 February 2004 20:57:03 Subject : [Histonet] The right processor for ISH I sent this e-mail out on Christmas Day but didn't get too many >responses. > >Can anyone suggest the right embedding machine for small scale ISH >studies. My museum is interested in getting one and we are looking >around for the right price/protocol. So far we've been embedding by >hand, but it would be fun to have a machine do the solvent and >paraplast changes instead. > -- Personalised email by http://another.com From brett_connolly <@t> merck.com Wed Feb 4 09:34:57 2004 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Phosphorylated Tau antibodies Message-ID: Has anyone found a source for anti-phosphorylated Tau antibodies that can be used for IHC. I only see several for Westerns and immunoblotting. I'm looking for anti-Tau (s) phosphorylated at Ser 262, 396 and 404. Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From m.vankempen <@t> erasmusmc.nl Wed Feb 4 09:52:02 2004 From: m.vankempen <@t> erasmusmc.nl (m. van mkempen) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Embryonic Endothelial Marker Message-ID: <40211522.EE1C0455@erasmusmc.nl> Dear all, I am a PhD student at the Erasmus MC in Rotterdam and I am trying to isolate embryonic endothelial cells from mouse lungs and set primary cultures. At the moment I have a couple of cultures that I have isolated by using a Percoll gradient. To identify these type of cells I have been using CD31 and Fli-1 antibodies in immunostainings but they did not reveal to be such good markers. I was woundering if anyone could sugest other maker/antibodies that would work weel on embryonic endothelial cells. Thanks in advance for all your suggestions! Kind regards. Marta -- ---------------------------- *- mkempen -* MAILTO:m.vankempen@erasmusmc.nl ----------------------------------------- From ahorstma <@t> waldenu.edu Wed Feb 4 11:02:29 2004 From: ahorstma <@t> waldenu.edu (Anne M. Horstmann) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] question/help-Marking ink References: Message-ID: <006901c3eb40$af161480$8519f7a5@anne> The best marking ink is from Delasco Dermatology Products. Their tissue stain is fantastic we have been using it for over 12 years with no problems. They have 5 colors including Blue, Black, Red, Yellow, and Green. The stain does not run during processing and not problems cutting. 1-800-831-6273 Hope this helps, Anne ----- Original Message ----- From: "Dunn-Jena, Patsy A" To: Sent: Tuesday, February 03, 2004 3:44 PM Subject: [Histonet] question/help-Marking ink > > Normally do Bone work in the laboratory, but recently did some skin for an investigator and wished afterwards that we had marked the cut surface. Can anybody please give some suggestions on where to locate marking ink? I would really appreciate it. > > Patsy Dunn-Jena, RVT, LAT, HT (ASCP) > Indiana University School of Dentistry > Mineralized Tissue and Histology Research Laboratory > 1121 West Michigan Street, Room 238 > Indianapolis, IN 46202 > (317) 274-0544 > padunnje@iupui.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barry.R.Rittman <@t> uth.tmc.edu Wed Feb 4 11:40:29 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Control tissues. Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FC3E@UTHEVS3.mail.uthouston.edu> I have been asked to approach the Histonet readers to see if y'all know of any suppliers of control tissue, Amyloid, bacteria in tissue etc. Thanks Barry Phone: 713-500-4134 From Patty.Lott <@t> ORTHO.UAB.EDU Wed Feb 4 12:00:04 2004 From: Patty.Lott <@t> ORTHO.UAB.EDU (Patty Lott) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] anti-collagen 2 Message-ID: <85F6C7A1330E794DB8540AFD001CC77E027130@ROSCO> Does anyone know of a supplier for an antibody to collagen 2? We would like to perform immunohistochemistry of FFPE tissues! Thanks in advance! Patty Lott, Laboratory Supervisor Orthopaedic Research Laboratory Center for Metabolic Bone Disease Laboratory University of Alabama at Birmingham LHRB B37 0007 1919 7th Ave. South Birmingham, AL 35294 (205) 934-2007 From jcline <@t> wchsys.org Wed Feb 4 12:33:45 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Control tissues. References: <566FB0B522443D43AF02D2ADBE35A6F077FC3E@UTHEVS3.mail.uthouston.edu> Message-ID: <002001c3eb4d$6c763080$1d2a14ac@wchsys.org> I geat my controls from Histology Outlet Mall Enterprises (H.O.M.E.) 800-474-9477 ----- Original Message ----- From: "Barry R Rittman" To: Sent: Wednesday, February 04, 2004 12:40 PM Subject: [Histonet] Control tissues. I have been asked to approach the Histonet readers to see if y'all know of any suppliers of control tissue, Amyloid, bacteria in tissue etc. Thanks Barry Phone: 713-500-4134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From huffpw <@t> uleth.ca Wed Feb 4 12:57:25 2004 From: huffpw <@t> uleth.ca (Phillip Huff) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Adipocyte- and Preadipocyte-Specific Antibody Search Message-ID: <50236.166.124.185.50.1075921045.squirrel@webmail.uleth.ca> I would like to have the advice of the histonet community on recommended antibodies against preadipocytes and adipocytes that people have had success with. Currently we are using a mouse tissues. I would like to use preadipocyte factor-1 for an anti-preadipocyte antibody, but would like to know if anyone has had success with other antibodies. In addition, can anyone direct me to sources (commercial or otherwise) of pref-1 antibody that they have found, other that Alpha Diagnostic, which is the only one I am aware of. As for an adipocyte-specific antibody, any recommendations would be nice. I would like to use ACRP30 but would appreciate any recommendations on other antibodies and sources. Thank you in advance for your help, Phillip Huff From SCheasty <@t> ahs.llumc.edu Wed Feb 4 15:42:34 2004 From: SCheasty <@t> ahs.llumc.edu (Cheasty, Sandra) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Does Tape Coverslipper Work for Cytology Slides? Message-ID: <2E50F33F91EEDA46A77BC3B2575BB09105875A@mars.llumc.edu> Hello again... The Cytopathologist here heard that the "waviness" of the tape coverslip will cause the cytologists to over-call the Pap smears. (Most of our Paps are Thin Prep) Does anyone knows of a cytology lab that uses the Sakura Tape coverslipper for paps? I would like to either confirm or dispel this rumor. Thanks, Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From garygill <@t> dcla.com Wed Feb 4 15:49:47 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Does Tape Coverslipper Work for Cytology Slides? Message-ID: Urban legend. There are numerous technical and optical reasons that contradict using tape coverslipper, but overcalling Paps is not one of them. If the distortion were so bad, one shouldn't attempt to interpret the cells in the first place. Gary Gill -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Wednesday, February 04, 2004 4:43 PM To: HistoNet (E-mail) Subject: [Histonet] Does Tape Coverslipper Work for Cytology Slides? Hello again... The Cytopathologist here heard that the "waviness" of the tape coverslip will cause the cytologists to over-call the Pap smears. (Most of our Paps are Thin Prep) Does anyone knows of a cytology lab that uses the Sakura Tape coverslipper for paps? I would like to either confirm or dispel this rumor. Thanks, Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BBenish <@t> celgene.com Wed Feb 4 16:21:41 2004 From: BBenish <@t> celgene.com (Brent Benish) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] immunoflourescent positive signal around tissue edges only Message-ID: <13A1ABE4769DD31192AC009027DE945D02F7ED89@MAIL> Having an issue where my liver tissue only has positive immunofluorescent labeling near the lateral edges of the tissue. As I look medially, the positive signal is lost. This effect is dramatic in paraffin embedded tissues, and less dramatic with frozen sections that are postfixed on slides with 4% para. Sections are covered with solutions for all protocol steps, and are not drying out. Any ideas about why only the outer edges (5 cells deep) of the liver label properly??? Thanks Brent ********************************************************* THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR INDIVIDUALS NAMED ABOVE. If the reader is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please reply to the sender to notify us of the error and delete the original message. Thank You. ********************************************************* From scoop <@t> mail.nih.gov Wed Feb 4 22:02:47 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] thanks for tissue marking dye help Message-ID: Thanks to everyone who gave me so much help finding an appropriate tissue marking dye (and also suggesting alternatives) for my mouse spinal cords! Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From bruyntjes <@t> voeding.tno.nl Thu Feb 5 02:51:43 2004 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] (no subject) Message-ID: <3B070848E7C2204F9DEB8BCFD76772800210D595@ntexch1.voeding.tno.nl> Hi all Is anyone of you familiar with a marker for apoptosis called "Cytodeath" (M30) from Roche on rat tissues? Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From SURGPATH19 <@t> aol.com Thu Feb 5 05:20:09 2004 From: SURGPATH19 <@t> aol.com (SURGPATH19@aol.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Slide Labeling Makers Message-ID: <0B03A508.67705189.0C1B89D8@aol.com> Hello Histoneters, Would someone be so kind as to forward me the information once again on the new slide labeling markers that has been discussed? Thanks for the help. Penny Duvall Mercy Hospital From Loralee_Gehan <@t> URMC.Rochester.edu Thu Feb 5 07:00:22 2004 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] anti-collagen 2 Message-ID: <95774A6A6036D411AFEA00D0B73C8643088804D4@exmc3.urmc.rochester.edu> We have been using Collagen Type II from NeoMarkers. Which I think that you buy through Lab Vision. Cat# ms-235--p. You have to use pepsin digestion. We have used it on Mouse and rabbit with much success at about a 1/40 dilution. But according to the data sheet it works in human, rat, cow and chicken as well. Any questions let me know. Loralee Gehan, BA, HTL (ASCP) Orthopaedics Research Lab University of Rochester Medical Center > ---------- > From: Patty Lott > Sent: Wednesday, February 4, 2004 1:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] anti-collagen 2 > > Does anyone know of a supplier for an antibody to collagen 2? We would > like > to perform immunohistochemistry of FFPE tissues! > > Thanks in advance! > > > > Patty Lott, Laboratory Supervisor > > Orthopaedic Research Laboratory > > Center for Metabolic Bone Disease Laboratory > > University of Alabama at Birmingham > > LHRB B37 0007 > > 1919 7th Ave. South > > Birmingham, AL 35294 > > (205) 934-2007 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From j.p.g.ooms <@t> zonnet.nl Thu Feb 5 07:29:42 2004 From: j.p.g.ooms <@t> zonnet.nl (Hans Ooms) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] histolab-organisation Message-ID: <001001c3ebec$1da92760$9370a63e@st2c8pdli7fy3i> Dear fellow-histonetters, I've had some discussion about the following two subjects : First : Every histologylab has its own way of tissueprocessing, as we all know; one lab will choose paraffinesectioning and mounting performed by one tech, the other chooses for sectioning by one tech, supported by a second tech who will mount the sections. (using waterbaths), i.e. one microtome, two techs. Does anyone of you have experience with both methods and what method is the most efficient? I've had experience with both and I think the latter is, but had some arguing with colleagues about this subject. Second : We use waterbaths to stretch paraffinsections and put some glycerine (app. 10 ml : 1000 ml water) in the bath for better mounting. The results are usually satisfying, except for very greasy tissue (lumps, mainly). Some labs use waterbaths and prepared slides (manually putting a film of glycerine on it) resulting in more background staining, depending the concentration of glycerine. Does anyone know a method which provides great mounting results, fairly no loss of tissue and absence of background staining ? Thanks very much in advance !! Hans Ooms Histotech The Netherlands From JWEEMS <@t> sjha.org Thu Feb 5 08:49:40 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] OT -help! Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD62C0@exch2.sjha.org> Sorry everyone, but I've lost Pricilla Delventhal! Pricilla, will you respond ASAP please? Thanks, Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From RBARNHART <@t> summithealth.org Thu Feb 5 09:27:50 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Safety Message-ID: After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org From thallada <@t> noch.org Thu Feb 5 09:29:49 2004 From: thallada <@t> noch.org (Hallada, Teri) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Cerner vs Meditech Message-ID: I was wondering if anyone could offer advice in choosing between Cerner and Meditech AP systems. We are currently using Tamtron (and hope to continue). The hospital is in the process of choosing a new HIS and we may be asked to utilize one of these two systems. Thanks. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. From gudrun.lang <@t> aon.at Thu Feb 5 10:09:25 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] histolab-organisation References: <001001c3ebec$1da92760$9370a63e@st2c8pdli7fy3i> Message-ID: <004901c3ec02$6ca813b0$eeeea8c0@SERVER> Hans, In our lab we use sliding microtoms and one person does the sectioning and mounting. Our waterbaths are filles with pure aqua dest., without any additive, at ca. 45 degrees. Most of the slides are untreated. The sections are 3 ?m thick. The slides dry 25 minutes in a drying chamber with air circulation at 60 degrees. Then we put them (warm) 3 x 5 min in bioclear and so on. We have almost no lost of sections and because of the lack of any additive no background stain. We loose most of the sections, when the drying-time is not long enough. How do you treat your slides after sectioning? Gudrun Lang, gneral hospital Linz, Austria ----- Original Message ----- From: "Hans Ooms" To: Sent: Thursday, February 05, 2004 2:29 PM Subject: [Histonet] histolab-organisation Dear fellow-histonetters, I've had some discussion about the following two subjects : First : Every histologylab has its own way of tissueprocessing, as we all know; one lab will choose paraffinesectioning and mounting performed by one tech, the other chooses for sectioning by one tech, supported by a second tech who will mount the sections. (using waterbaths), i.e. one microtome, two techs. Does anyone of you have experience with both methods and what method is the most efficient? I've had experience with both and I think the latter is, but had some arguing with colleagues about this subject. Second : We use waterbaths to stretch paraffinsections and put some glycerine (app. 10 ml : 1000 ml water) in the bath for better mounting. The results are usually satisfying, except for very greasy tissue (lumps, mainly). Some labs use waterbaths and prepared slides (manually putting a film of glycerine on it) resulting in more background staining, depending the concentration of glycerine. Does anyone know a method which provides great mounting results, fairly no loss of tissue and absence of background staining ? Thanks very much in advance !! Hans Ooms Histotech The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gudrun.lang <@t> aon.at Thu Feb 5 10:23:36 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] further training events? Message-ID: <005401c3ec04$683a9580$eeeea8c0@SERVER> Dear histonetters! My pathologist asked me to look for histotech trainings. (I don't know, how to say it better in English). Any event, where interested histotechs can get informations about new techniques, histology-knowledge e.g. in U.K. (USA is unfortunatly too expensive for our small hospital.) Can anybody tell me an adress, where I can ask for such events? For example a pathology-association or histotech-association? Thanks in advance Gudrun Lang Austria From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Feb 5 10:34:06 2004 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Safety Message-ID: <3D502BBF5356D31184650090275B750D0346C6E1@mail.cooley-dickinson.org> Becky, All of the safety discussions have really gotten my attention as well. I work at a small hospital with 3 techs and 3 pathologists. In a very small room. I just joined a year ago. I've been in histology for 12 yrs. As far as dumping solutions down the drain, try enlisting the help of your institution's safety officer. Try contacting your local water/sewer department. When I arrived they were still dumping silver, gold, chromic acid and others down the drain. The excuse was always "it's such a small quantity." I am not comfortable with heavy metals and carcinogens going into our water system. Thankfully I got the extra help I needed from outside my department to back up that decision. As for the fumes, we monitor formalin and xylene. This year we are starting ethanol and methanol as well. I use gloves when coverslipping. Like yourself, we have a lot of issues that need to be addressed. Not always an easy task, given the fact that so many people want to do things their way. I'm trying to get rid of our glass specimen jars with cork tops! Been an uphill battle for years... Good luck, Stacy McLaughlin -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Thursday, February 05, 2004 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From garsha <@t> itg.uiuc.edu Thu Feb 5 11:22:48 2004 From: garsha <@t> itg.uiuc.edu (Karl Garsha) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] anti-myelin (mouse brain) antibody? Message-ID: <40227BE8.9000201@itg.uiuc.edu> Greetings histologists, Can someone point me in the right direction for a good, specific monoclonal antibody to myelin in mouse brains? Thanks in advance for any tips. Cheers, Karl G. -- Karl Garsha Light Microscopy Specialist Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 North Mathews Avenue Urbana, IL 61801 Office: B650J Phone: 217.244.6292 Fax: 217.244.6219 Mobile: 217.390.1874 www.itg.uiuc.edu From MAUGER <@t> email.chop.edu Thu Feb 5 11:51:05 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Cerner vs Meditech Message-ID: They have used meditech here for years and don,t like it. We are looking at Cerner, which is windows based, and much easier to use and interface. Meditech is archaic key funtion DOS like system. >>> "Hallada, Teri" 02/05/04 10:29AM >>> I was wondering if anyone could offer advice in choosing between Cerner and Meditech AP systems. We are currently using Tamtron (and hope to continue). The hospital is in the process of choosing a new HIS and we may be asked to utilize one of these two systems. Thanks. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Thu Feb 5 12:06:38 2004 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Cerner vs Meditech Message-ID: Our entire hospital has Meditech here and love it. Meditech Client Server is windows based (that is what we have) but Magic is like a DOS system. I have never used but seen magic and was not impressed with it. I was on our Meditech team and set up the pathology module. Out of the 3 other system we have had, I like Meditech the best. I think it is very user friendly, point and click. There are a lot of ways to customize to suit your needs. If you need any more information let me know. I can put together some screen prints if it would help. Becky rbarnhart@summithealth.org >>> "Joanne Mauger" 02/05/04 12:51PM >>> They have used meditech here for years and don,t like it. We are looking at Cerner, which is windows based, and much easier to use and interface. Meditech is archaic key funtion DOS like system. >>> "Hallada, Teri" 02/05/04 10:29AM >>> I was wondering if anyone could offer advice in choosing between Cerner and Meditech AP systems. We are currently using Tamtron (and hope to continue). The hospital is in the process of choosing a new HIS and we may be asked to utilize one of these two systems. Thanks. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Thu Feb 5 12:13:59 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Cerner vs Meditech Message-ID: We also use Meditech, but ours is windows based and very easy to use. I think you need to contact all of them and see what best suits your needs. Good luck, Juan -----Original Message----- From: Joanne Mauger [mailto:MAUGER@email.chop.edu] Sent: Thu 2/5/2004 11:51 AM To: histonet@lists.utsouthwestern.edu; thallada@noch.org Cc: Subject: Re: [Histonet] Cerner vs Meditech They have used meditech here for years and don,t like it. We are looking at Cerner, which is windows based, and much easier to use and interface. Meditech is archaic key funtion DOS like system. >>> "Hallada, Teri" 02/05/04 10:29AM >>> I was wondering if anyone could offer advice in choosing between Cerner and Meditech AP systems. We are currently using Tamtron (and hope to continue). The hospital is in the process of choosing a new HIS and we may be asked to utilize one of these two systems. Thanks. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Feb 5 12:20:18 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Innogenetics Message-ID: Do any of our European colleagues have contact information (e-mail address) for a biotech company in Belgium called "Innogenetics"? Thank you. Richard Cartun From Jacquie.Mack <@t> CLS.ab.ca Thu Feb 5 12:12:47 2004 From: Jacquie.Mack <@t> CLS.ab.ca (Jacquie.Mack@CLS.ab.ca) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] unsubscribe Message-ID: <30C050525B881C4AAFF41E6D16543E6803488A54@mail3.cls.ab.ca> unsubscribe Jacqueline Mack Tech III , Anatomic Pathology Foothills Medical Center Calgary Laboratory Services ( (403)-944-4162 fax: (403)-270-4093 pager: 212-8223 # 0540 * Ja cquie.Mack@CLS.ab.ca Laboratory Medicine Enhancing Your Health Care From LINDA.MARGRAF <@t> childrens.com Thu Feb 5 12:26:48 2004 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] immunofluorescent antibody tissue controls Message-ID: Dear Histonetters We are continually running low on control tissue for our immunofluorescent (IF) stains for IgG, IgA, IgM, and C3 that we run on kidney biopsies. Our pediatric population rarely has a kidney removed for diseases with antibody deposits and none of our neighboring hospitals has any control tissue to spare. DOes anyone know of a good commercial source for positive IF control tissue for Igs and C3? Thanks Linda M Histonet administrator Director of Anatomic Pathology Children's Medical Center of Dallas From Rcartun <@t> harthosp.org Thu Feb 5 12:30:46 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Freezer Message-ID: I am looking for a "small" -70 degree C. freezer for our frozen section room in the operating room. Any suggestions? Thank you. Richard Cartun From susan.wells <@t> bms.com Thu Feb 5 12:42:11 2004 From: susan.wells <@t> bms.com (Susan Q Wells) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] immunofluorescent antibody tissue controls References: Message-ID: <40228E83.584EE1D7@bms.com> I'd also like responses to this.I haven't been able to locate a control either. Thanks in advance. Sue Wells LINDA MARGRAF wrote: > Dear Histonetters > We are continually running low on control tissue for our > immunofluorescent (IF) stains for IgG, IgA, IgM, and C3 that we run on > kidney biopsies. Our pediatric population rarely has a kidney removed > for diseases with antibody deposits and none of our neighboring > hospitals has any control tissue to spare. DOes anyone know of a good > commercial source for positive IF control tissue for Igs and C3? > Thanks > Linda M > Histonet administrator > Director of Anatomic Pathology > Children's Medical Center of Dallas > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BRobert <@t> ameripath.com Thu Feb 5 12:52:25 2004 From: BRobert <@t> ameripath.com (BRobert@ameripath.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Does Tape Coverslipper Work for Cytology Slides? Message-ID: We do use the Tissue Tek tape coverslipper for cyto slides. Everybody seems happy with it. B. Robert APMG, Los Gatos, CA -----Original Message----- From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu] Sent: Wednesday, February 04, 2004 1:43 PM To: HistoNet (E-mail) Subject: [Histonet] Does Tape Coverslipper Work for Cytology Slides? Hello again... The Cytopathologist here heard that the "waviness" of the tape coverslip will cause the cytologists to over-call the Pap smears. (Most of our Paps are Thin Prep) Does anyone knows of a cytology lab that uses the Sakura Tape coverslipper for paps? I would like to either confirm or dispel this rumor. Thanks, Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbrown <@t> sparks.org Thu Feb 5 13:02:36 2004 From: dbrown <@t> sparks.org (dbrown@sparks.org) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Proficiency/Competnecy Histology Tests Message-ID: Does anyone out there know of a source for proficiciency tests for HistoTechs? I know they are out there for MedTechs but I haven't had any luck trying the internet. Thanks for your help.... From japoteete <@t> saintfrancis.com Thu Feb 5 12:58:17 2004 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] GMS Stain Message-ID: I know there is something to use when the slides get left in the silver solution to long but have forgotten. Can someone refresh my memory? Thanks ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From JWEEMS <@t> sjha.org Thu Feb 5 13:21:44 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] GMS Stain Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD62C8@exch2.sjha.org> Perm solution will do the trick. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Poteete, Jacquie A. Sent: Thursday, February 05, 2004 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS Stain I know there is something to use when the slides get left in the silver solution to long but have forgotten. Can someone refresh my memory? Thanks ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From SohrabB <@t> wmmcpo.ah.org Thu Feb 5 13:23:35 2004 From: SohrabB <@t> wmmcpo.ah.org (Behnaz Sohrab) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] NEW ANTIBODY Message-ID: Is anyone of you familiar with ZAP 70 antibody ? And if yes , What is your protocol ? Thanks From nick.kirk3 <@t> btopenworld.com Thu Feb 5 13:39:43 2004 From: nick.kirk3 <@t> btopenworld.com (=?iso-8859-1?q?NICK=20KIRK?=) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] further training events? In-Reply-To: <005401c3ec04$683a9580$eeeea8c0@SERVER> Message-ID: <20040205193943.68771.qmail@web86309.mail.ukl.yahoo.com> Gudrun In the UK we have a Professional body called the Institute of Biomedical Science. Within this organisation are numerous Cellular Pathology discussion groups who hold meetings/lectures as well as the IBMS itself which holds a biennial Scientific Conference (the next one is in 2006). If you go to the IBMS website www.ibms.org, there are listings of events, courses etc as well as links to other organisations and providers of educational activities. We also have another website called www.ibmsscience.org, where you will find articles as well as scientific information. Hope that is of some use. If I can be of any further help, just ask. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England Gudrun Lang wrote: Dear histonetters! My pathologist asked me to look for histotech trainings. (I don't know, how to say it better in English). Any event, where interested histotechs can get informations about new techniques, histology-knowledge e.g. in U.K. (USA is unfortunatly too expensive for our small hospital.) Can anybody tell me an adress, where I can ask for such events? For example a pathology-association or histotech-association? Thanks in advance Gudrun Lang Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Feb 5 14:38:42 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:32 2005 Subject: Fw: [Histonet] GMS Stain Message-ID: <003701c3ec28$0ba1b5a0$1d2a14ac@wchsys.org> I use a coplin jar of distilled water and I add two -three drops of clorox and then dip the slides until the excess silver comes off. Rinse well in water. ----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PM Subject: [Histonet] GMS Stain > I know there is something to use when the slides get left in the silver > solution to long but have forgotten. Can someone refresh my memory? > Thanks > > > > > ********* Email Confidentiality Statement ********* > Visit http://www.saintfrancis.com/emailconf.asp > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From pruegg <@t> colobio.com Thu Feb 5 14:39:31 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] anti-collagen 2 In-Reply-To: <85F6C7A1330E794DB8540AFD001CC77E027130@ROSCO> Message-ID: I get all my collagen antibodies from the U of Iowa Hybridoma BanK. They are cheap and very good quality. Developmental Studies BioSciences University of Iowa 007 BBE Iowa City, IA 52242-1324 319-335-3826 dshb@uiowa.edu I use pepsin digestion (premade from Invitrogen (previosly sold by Research Genetics) 10 min. at 37dc. The antibody I buy is supernate and I use it neat. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patty Lott Sent: Wednesday, February 04, 2004 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] anti-collagen 2 Does anyone know of a supplier for an antibody to collagen 2? We would like to perform immunohistochemistry of FFPE tissues! Thanks in advance! Patty Lott, Laboratory Supervisor Orthopaedic Research Laboratory Center for Metabolic Bone Disease Laboratory University of Alabama at Birmingham LHRB B37 0007 1919 7th Ave. South Birmingham, AL 35294 (205) 934-2007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Thu Feb 5 14:47:09 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] apoptosis vs necrosis In-Reply-To: <3B070848E7C2204F9DEB8BCFD76772800210D595@ntexch1.voeding.tno.nl> Message-ID: I am charged with trying to label apoptotic cells in mouse tissue that is necrotic due to the bacterial infection it has been exposed to. I have previously worked extensively with the tunnel assay for apoptosis and could not ever tell the difference between apoptotic cells and necrotic cells. Is there something new that would make this distinctsion? Please advise -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bruijntjes, J.P. Sent: Thursday, February 05, 2004 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi all Is anyone of you familiar with a marker for apoptosis called "Cytodeath" (M30) from Roche on rat tissues? Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Feb 5 14:59:25 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Re: [IHCRG] apoptosis vs necrosis Message-ID: Anti-active Caspase 3 will label only apoptotic cells, and NOT the necrotic cells. I prefer Caspase to TUNEL for that very reason - TUNEL stains everything dying or dead. Jackie O' "Patsy Ruegg" 02/05/2004 02:47 PM To: "Bruijntjes, J.P." , cc: "Ihcrg@Yahoogroups. Com" Subject: [IHCRG] apoptosis vs necrosis I am charged with trying to label apoptotic cells in mouse tissue that is necrotic due to the bacterial infection it has been exposed to. I have previously worked extensively with the tunnel assay for apoptosis and could not ever tell the difference between apoptotic cells and necrotic cells. Is there something new that would make this distinctsion? Please advise -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bruijntjes, J.P. Sent: Thursday, February 05, 2004 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi all Is anyone of you familiar with a marker for apoptosis called "Cytodeath" (M30) from Roche on rat tissues? Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Yahoo! Groups Links <*> To visit your group on the web, go to: http://groups.yahoo.com/group/IHCRG/ <*> To unsubscribe from this group, send an email to: IHCRG-unsubscribe@yahoogroups.com <*> Your use of Yahoo! Groups is subject to: http://docs.yahoo.com/info/terms/ From tpmorken <@t> labvision.com Thu Feb 5 15:03:43 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Safety Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2035827@usca0082k08.labvision.apogent.com> Becky, I would suggest you talk with your fire marshal about that 55 gallon drum - I seriously doubt that quantity will be allowed in your lab - it certainly isn't under CAP guidelines. Tim Morken After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Thu Feb 5 15:08:28 2004 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] immunofluorescent antibody tissue controls Message-ID: <38F928258973D711977200D0B7DB9D74018DE3DF@mail.umc.ufl.edu> Linda, Since we on an immunofluorescent thread...would any one be willing to share their protocol for IF on kidney biopsies (IgG, IgA, IgM, C3) including source/dilutions of antibodies? Thanks. Becky Garrison Pathology Shands Jacksonville 904-244-6237 -----Original Message----- From: LINDA MARGRAF [mailto:LINDA.MARGRAF@childrens.com] Sent: Thursday, February 05, 2004 1:27 PM To: Histonet@lists.utsouthwestern.edu Cc: MICHELLE MCNEESE Subject: [Histonet] immunofluorescent antibody tissue controls Dear Histonetters We are continually running low on control tissue for our immunofluorescent (IF) stains for IgG, IgA, IgM, and C3 that we run on kidney biopsies. Our pediatric population rarely has a kidney removed for diseases with antibody deposits and none of our neighboring hospitals has any control tissue to spare. DOes anyone know of a good commercial source for positive IF control tissue for Igs and C3? Thanks Linda M Histonet administrator Director of Anatomic Pathology Children's Medical Center of Dallas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peptolab <@t> hamptons.com Thu Feb 5 15:37:08 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Preadipocytes Message-ID: <001d01c3ec30$369537c0$95a5bd18@JEFF> Phil- In the literature, I have maintained that the CD34+ interstitial dendritic fibroblast seen as the predominant stromal cell in fat, dermis, and most collagenous connective tissue is, among other things, a preadipocyte. If you like, do a medline search for Silverman JS and send me your address if any titles interest you. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital Bay Shore, New York USA From Luis.Chiriboga <@t> med.nyu.edu Thu Feb 5 15:59:33 2004 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] RE: [IHCRG] apoptosis vs necrosis In-Reply-To: Message-ID: check out the following article "comparison of IHC for Activated caspase3 and cleaved cytokeratin 18 with the tunel method for quantification of apoptosis in histological sections of PC-3 Subcutaneous xenografts" J Path 199:221-228 2003 -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Thursday, February 05, 2004 3:47 PM To: Bruijntjes, J.P.; histonet@lists.utsouthwestern.edu Cc: Ihcrg@Yahoogroups. Com Subject: [IHCRG] apoptosis vs necrosis I am charged with trying to label apoptotic cells in mouse tissue that is necrotic due to the bacterial infection it has been exposed to. I have previously worked extensively with the tunnel assay for apoptosis and could not ever tell the difference between apoptotic cells and necrotic cells. Is there something new that would make this distinctsion? Please advise -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bruijntjes, J.P. Sent: Thursday, February 05, 2004 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi all Is anyone of you familiar with a marker for apoptosis called "Cytodeath" (M30) from Roche on rat tissues? Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Yahoo! Groups Links <*> To visit your group on the web, go to: http://groups.yahoo.com/group/IHCRG/ <*> To unsubscribe from this group, send an email to: IHCRG-unsubscribe@yahoogroups.com <*> Your use of Yahoo! Groups is subject to: http://docs.yahoo.com/info/terms/ From csawrenk <@t> bccancer.bc.ca Thu Feb 5 16:02:25 2004 From: csawrenk <@t> bccancer.bc.ca (Christina Sawrenko) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Antibodies to NFkB, p57, MDM2 Message-ID: <6BAF4D075F07D411B30900508B94CBA09C6D2E@SERVER20> Hello, Our pathologists have asked us to investigate the following antibodies: p65 (RelA), cRel (these are part of the Nuclear Factor kB family) p57 MDM2 We would be using the antibodies on formalin fixed, paraffin processed human tissue. Does anyone in Histoland have any experience with any of these antibodies? Which clone(s) are you using; who is your supplier; and do you have any tips to pass along regarding the technical details (antigen retrieval, dilution etc). Thanks in advance for any help. You are always a great resource. Chris Sawrenko Histopathology BC Cancer Agency Vancouver BC, Canada From peptolab <@t> hamptons.com Thu Feb 5 15:48:02 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Microtomy teamwork/glycerine ?Jelly Message-ID: <002f01c3ec31$ec1975b0$95a5bd18@JEFF> Hoi Hans- I have never heard of two-person microtomy- where one handles only the ribbon on the waterbath. I would like to see that at full speed. Is this common in NL? What town/city do you practice in? (I love NL and visit often- a lekker ding). For section adherence, I use nothing but fresh distilled water each day. 99% of the tissue stays on the slide after only 15 minutes in the 65 degree C oven of my Leica stainer. Troublesome tissues- (bone, brain, little else) get floated onto Plus charge silanized slides. By glycerine- I assume you mean Mayers albumen- (glycerine and egg white with thymol) ? Groetjes van Jeff Silverman Histotech 32 years Southside Hospital Bay Shore, New York USA From laurie.colbert <@t> huntingtonhospital.com Thu Feb 5 16:13:05 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Safety Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0CA7@EXCHANGE1.huntingtonhospital.com> We have a 55 gallon drum that is enclosed in a flammable cabinet. The flammable cabinet is also vented to the outside. Although we are now recycling most of our reagents, we are able to dump formalin, alcohol, and xylene all together into this one big drum and have it hauled off. It makes it very easy on us, and we have never had any problems with CAP or safety inspections. As far as fumes and smells go, we have an air filter that we got from Creative Waste Solutions, Inc. It is supposed to be good at reducing not only the smells but also the exposure to the chemicals. I use the air filter in the grossing area and several of us noticed a reduction in the formalin fumes. It is also supposed to work with xylene. I am not affilitate with Creative Waste and if you're interested, their number is (888) 795-8300. The people there are great to work with. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Thursday, February 05, 2004 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Feb 5 16:16:52 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] immunofluorescent antibody tissue controls Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E124@simba.kids> Becky, >From our manual: Document Procedure: Immunofluorescence Frozen Section Principle: Immunofluorescence is part of immunohistochemistry that combines the basic principles of histochemistry with the high degree of molecular specificity inherent in the antigen-antibody reaction. In tissue there are essentially two forms of fluorescence, primary or autofluorescence that can be seen in collagen fibres (blue green) or in lipids (shades of yellow) and secondary fluorescence where fluorochrome dyes applied and the created immuncomplex fluoresces when activated by UV light. Localisation is revealed by labelling one of the components of the staining reaction with a fluorochrome dye. Flourescence isothyocyanate (FITC) is the commonly used dye for this purpose. Purpose: To demonstrate the presence of IgA, IgG, IgM, C3c, C1q and Fibrinogen antigens in skin lesions and renal biopsies by employing fluorescence technique instead of immunoperoxidase. Controls: Not used with frozen specimens Reagents: 1. Tris Buffer - as used in immunoperoxidase 2. Antibodies: a. IgG-FITC Dako F0202 b. IgA-FITC Dako F0204 c. IgM-FITC Dako F0203 d. C3c-FITC Dako F0201 e. C1q-FITC Dako F0254 f. Fibrinogen-FITC Dako F0111 Dilute antibodies 1/10 in Antibody diluent 3. 1% Toluidine Blue 4. Permafluor mounting medium Procedure: 1. Freeze tissues as rapidly as possible. 2. Trim and cut first section, stain with 1% Toluidine blue to assess the presence of glomeruli 3. Cut 7 sections at 5 microns, fix 7th section in methanol for H&E staining 4. Air dry sections 15minutes. 5. Place sections in Tris buffer at 37oC for 7 minutes 6. Apply diluted antibodies to each section and incubate at room temperature 60min. Cover slides from light 7. Place slides in Tris buffer at 37oC for 7 minutes. 8. Apply drops of Permafluor & coverslip - dry for 20 mins. Slides mounted with Permafluor are stable at RT -Long term storage at 4?C. Reference: Henwood, Harmata & Scott, (1983) "A Control for Routine Immunofluorescent Histochemistry". Aust J Med Lab Sc 4:187-188. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Garrison, Becky [mailto:becky.garrison@jax.ufl.edu] Sent: Friday, 6 February 2004 8:08 AM To: 'LINDA MARGRAF'; Histonet@lists.utsouthwestern.edu Cc: MICHELLE MCNEESE Subject: RE: [Histonet] immunofluorescent antibody tissue controls Linda, Since we on an immunofluorescent thread...would any one be willing to share their protocol for IF on kidney biopsies (IgG, IgA, IgM, C3) including source/dilutions of antibodies? Thanks. Becky Garrison Pathology Shands Jacksonville 904-244-6237 -----Original Message----- From: LINDA MARGRAF [mailto:LINDA.MARGRAF@childrens.com] Sent: Thursday, February 05, 2004 1:27 PM To: Histonet@lists.utsouthwestern.edu Cc: MICHELLE MCNEESE Subject: [Histonet] immunofluorescent antibody tissue controls Dear Histonetters We are continually running low on control tissue for our immunofluorescent (IF) stains for IgG, IgA, IgM, and C3 that we run on kidney biopsies. Our pediatric population rarely has a kidney removed for diseases with antibody deposits and none of our neighboring hospitals has any control tissue to spare. DOes anyone know of a good commercial source for positive IF control tissue for Igs and C3? Thanks Linda M Histonet administrator Director of Anatomic Pathology Children's Medical Center of Dallas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From peptolab <@t> hamptons.com Thu Feb 5 16:10:02 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Chemical Safety Message-ID: <004201c3ec34$cf2b45c0$95a5bd18@JEFF> Rebecca- If you have installed engineering controls in the lab like fume hoods for transfering hazardous solvents and some employees refuse to use them, your lab or hospital's safety officer and lab administration need to counsel those individuals and begin progressive discipline for continued unsafe work practices. This assumes that you have a written chemical hygiene plan with specific PPE's and engineering controls designated for each hazardous task in the laboratory (task assessment). You should not be dumping methanol down the drain- ?does your hematology lab do that? It is on the DEC/EPA schedule as toxic. We are also careful that ethanol baths saturated with hazardous clearing agents are added to the waste clearing agents for disposal and not washed away. We use Americlear too for purging the processor and in the staining lines- but we use xylene to clear on the processor and as the last coverslip bath. Americlear is cool stuff-supposedly it has ANTITUMOR effects and has been fed to humans in clinical trials. Some people are violently allergic to it- though I have never met (or worked with someone) who is. Also, special stain waste that contains heavy metals like chromium, silver, mercury, or DAB should not be dumped down the drain. Hope this helps. Jeff Silverman Pathologists' Assistant/Safety Officer Southside Hospital Bay Shore, New York USA From peptolab <@t> hamptons.com Thu Feb 5 16:41:39 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Xylene or "solvents" a hazard? Message-ID: <006401c3ec39$3d1c4620$95a5bd18@JEFF> Very informative article Vinnie- but only 21 of the 125 exposed women were histotechs- it would be more useful to know how many of the 13 major and 5 minor malformations, the 17 fetal distress, the 9 premies, the 12 miscarriages (not much more than unexposed- 20% greater risk) , were in histotechs rather than the 104 persons in industry or other disciplines presuambly using methyl ethyl ketone, benzene, and who knows what else. This is not to say that I don't think pregnant techs should be rotated away from exposure. By the way- I read about the "horrors" of limonene- I love my limonene, have for 20+years, and I wonder how many labs use it versus aliphatics? Never met anyone who had a problem with it and visitors to the lab (courriers, other techs) love the smell- when you can smell it at all. Regards Jeff Silverman From peptolab <@t> hamptons.com Thu Feb 5 16:27:41 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Embryonic endothelium maker Message-ID: <005001c3ec37$46a23da0$95a5bd18@JEFF> Marta- I think CD34 human progenitor cell antigen (clone QBend10) would be better for embryonic endothelium than CD31 (PECAM Platelet Endothelial Cell Adhesion Molecule). groeten van Jeff Silverman Southside Hospital Bay Shore, New York USA From tpmorken <@t> labvision.com Thu Feb 5 17:44:00 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Safety Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA203582D@usca0082k08.labvision.apogent.com> Laurie wrote: <> Here is the pertinent section of the July 2003 CAP checklist (Applies to CAP-accredited labs only, a USA agency) having to do with flammable storage. Further below are all the flammable checklist items. Flammable storage *************************************************************** GEN.72050 Phase II N/A YES NO Are supplies of flammable and combustible liquids reasonable for the laboratory's needs, and are they properly stored? NOTE: In each laboratory area, up to 1 gallon of Class I, II, and IIIA liquids may be stored on open shelving for each 100 ft2 of space defined by fire-resistant walls/doors. Up to 2 gallons of Class I, II, and IIIA liquids may be stored in safety cans and safety cabinets for each 100 ft2. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). COMMENTARY: The supplies of flammable liquids were excessive or they were improperly stored. No more than 1 gallon of class I, II, and IIIA liquids may be stored on open shelving per 100 ft2 of space defined by fire-resistant walls/doors. No more than 2 gallons of class I, II, and IIIA liquids may be stored in safety cans and safety cabinets per 100 ft2 in any laboratory work area. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). REFERENCES: 1) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996; 2) National Fire Protection Association. Standard 99, chapter 10-7.2.2. Quincy, MA: NFPA, 2000. ***************************************************************** Can anyone say their lab meets this standard? It allows only a very small amount of flammables to be in the working lab area. Lets say you had a 55 gallon drum of flammables in your lab (where people are working) waiting to be carted off, or even recycled. If it is in a flammable cabinet and you have sprinklers you need a minimum of over 1300 square feet of lab space just for that (55/4 * 100). If you have more flammables you need even more space. This holds for any flammables in an area where people are working - for instance, those stored for use in processors, stainers etc. Not to mention those already in the processors and stainers. The only real solution to this is to have a dedicated storage area for bulk flammables that is isolated from the lab. Preferably one that is designed to contain/suppress a big fire. We had the "bunker" at one hosptial I worked at - an underground storage area with spark-proof electrical system and a bomb-proof door that was used for all bulk flamables storage. BUT, having said that, I have to say that in 8 CAP inspections I never had a CAP inspector even comment on this, despite being over the standard in almost every case. So maybe in the real world nobody really cares about it. Tim Morken All the flammable standards **************************************************************************** ********* GEN.72000 Phase I N/A YES NO Are safety cans used instead of glass bottles for volumes of flammable solvents larger than one quart (or larger than one pint for solvents that are highly volatile such as isopentane) if the purity required does not mandate glass storage? COMMENTARY: Safety cans should be used for bulk storage of flammable and combustible liquid (National Fire Protection Association classes I and II). Metal or DOT approved plastic containers provide an intermediate level of hazard containment between glass and safety cans. One pint of a highly volatile solvent, such as isopentane, stored in glass has about the same ignitability risk as 2 gallons stored in safety cans. Safety cans should be used instead of glass bottles if the purity required does not mandate glass storage. REFERENCE: National Fire Protection Association. Flammable and combustible liquids code. Standard 30, chapter 10-2.2.4.3. Quincy, MA: NFPA, 1996. **************************************************************************** ************** GEN.72050 Phase II N/A YES NO Are supplies of flammable and combustible liquids reasonable for the laboratory's needs, and are they properly stored? NOTE: In each laboratory area, up to 1 gallon of Class I, II, and IIIA liquids may be stored on open shelving for each 100 ft2 of space defined by fire-resistant walls/doors. Up to 2 gallons of Class I, II, and IIIA liquids may be stored in safety cans and safety cabinets for each 100 ft2. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). COMMENTARY: The supplies of flammable liquids were excessive or they were improperly stored. No more than 1 gallon of class I, II, and IIIA liquids may be stored on open shelving per 100 ft2 of space defined by fire-resistant walls/doors. No more than 2 gallons of class I, II, and IIIA liquids may be stored in safety cans and safety cabinets per 100 ft2 in any laboratory work area. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). REFERENCES: 1) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996; 2) National Fire Protection Association. Standard 99, chapter 10-7.2.2. Quincy, MA: NFPA, 2000. **************************************************************************** *************** GEN.72100 Phase II N/A YES NO Are storage areas and/or rooms where volatile solvents are used adequately ventilated? COMMENTARY: Areas where flammable liquids are used must be ventilated for protection of employee health, as well as fire prevention. Areas where flammable liquids are stored should be ventilated primarily for fire protection. Storage cabinets do not need to be vented, but if they are vented the duct system must be explosion proof. REFERENCE: National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996. **************************************************************************** *************** GEN.72150 Phase II N/A YES NO Are flammable or combustible liquids or gas cylinders positioned well away from open flame or other heat sources, not in corridors and not within exhaust canopies? COMMENTARY: Flammable or combustible liquids or gas cylinders must not be positioned near open flame, heat sources, in corridors, or within exhaust canopies. REFERENCE: National Fire Protection Association. Standard 99, chapter 10-7.2.4. Quincy, MA: NFPA, 2000. **REVISED** 07/31/2003 **************************************************************************** **************** GEN.72200 Phase I N/A YES NO If flammables are decanted from large drum (bulk) containers, is the secondary (receiving) container grounded? COMMENTARY: Transfer of flammable liquid from bulk storage containers should be made in storage rooms as described in NFPA 30, and the secondary container(s) should be grounded to prevent static electrical discharge if constructed of metal or other conductive material. REFERENCES: 1) National Fire Protection Association. Recommended practice on static electricity. Standard 77. Quincy, MA: NFPA, 1993; 2) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996. -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Thursday, February 05, 2004 2:13 PM To: Rebecca Barnhart; Histonet (E-mail) Subject: RE: [Histonet] Safety We have a 55 gallon drum that is enclosed in a flammable cabinet. The flammable cabinet is also vented to the outside. Although we are now recycling most of our reagents, we are able to dump formalin, alcohol, and xylene all together into this one big drum and have it hauled off. It makes it very easy on us, and we have never had any problems with CAP or safety inspections. As far as fumes and smells go, we have an air filter that we got from Creative Waste Solutions, Inc. It is supposed to be good at reducing not only the smells but also the exposure to the chemicals. I use the air filter in the grossing area and several of us noticed a reduction in the formalin fumes. It is also supposed to work with xylene. I am not affilitate with Creative Waste and if you're interested, their number is (888) 795-8300. The people there are great to work with. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Thursday, February 05, 2004 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bills <@t> icpmr.wsahs.nsw.gov.au Thu Feb 5 18:28:25 2004 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Xylene or "solvents" a hazard? In-Reply-To: <006401c3ec39$3d1c4620$95a5bd18@wsahs.nsw.gov.au> Message-ID: <000901c3ec48$22c14dc0$3187080a@wsahs.nsw.gov.au> Jeff, I agree with all you have said about the article, in my 38 years in the laboratory 34 in Histopathology, I have never known of a female staff member having any troubles with birth defects, however, we have had some who have experienced miscarriages. But they were usually staff who had not worked for long in the laboratory and the minute we knew they were pregnant they were moved to minimise any exposure. Limonene is now the bain of my life, I have become sensitised to the smell although I have had very limited exposure over the years. An allergy test gave results in about 15 seconds, with other related problems such as nausea, headaches, rash, and an hayfever type reaction. The only way I can stop the problem is with high does of antihistamine. I am still not convinced that it is so wonderful. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of peptolab Sent: Friday, 06 February 2004 9:42 AM To: dellav@musc.edu Cc: HistoNet Server Subject: [Histonet] Xylene or "solvents" a hazard? Very informative article Vinnie- but only 21 of the 125 exposed women were histotechs- it would be more useful to know how many of the 13 major and 5 minor malformations, the 17 fetal distress, the 9 premies, the 12 miscarriages (not much more than unexposed- 20% greater risk) , were in histotechs rather than the 104 persons in industry or other disciplines presuambly using methyl ethyl ketone, benzene, and who knows what else. This is not to say that I don't think pregnant techs should be rotated away from exposure. By the way- I read about the "horrors" of limonene- I love my limonene, have for 20+years, and I wonder how many labs use it versus aliphatics? Never met anyone who had a problem with it and visitors to the lab (courriers, other techs) love the smell- when you can smell it at all. Regards Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From nick.kirk3 <@t> btopenworld.com Thu Feb 5 22:36:52 2004 From: nick.kirk3 <@t> btopenworld.com (=?iso-8859-1?q?NICK=20KIRK?=) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet]Correction to further training events? In-Reply-To: <005401c3ec04$683a9580$eeeea8c0@SERVER> Message-ID: <20040206043652.47611.qmail@web86311.mail.ukl.yahoo.com> Gudren Sorry, I made a mistake with the dates for the Biennial Conference, I should have said that the next one is in 2005, not 2006. The website for the one held in October last year does have some details of some of the lectures on it. You can find it at www.ibmscongress.com. There is a Histology day meeting being held in Aylesbury Buckinghamshire in May of this year, as well as a weekend Histology/Cytology meeting being held in Hinckley, Leicestershire in October of this year. Both are easy to get to from local airports that have services from Austria. I can fowrard you the details of these events if you would like. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England Gudrun Lang wrote: Dear histonetters! My pathologist asked me to look for histotech trainings. (I don't know, how to say it better in English). Any event, where interested histotechs can get informations about new techniques, histology-knowledge e.g. in U.K. (USA is unfortunatly too expensive for our small hospital.) Can anybody tell me an adress, where I can ask for such events? For example a pathology-association or histotech-association? Thanks in advance Gudrun Lang Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Fri Feb 6 02:44:25 2004 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Freezer Message-ID: Dear Richard, We manufacture a -80?C small freezer, its prime function is for fast freezing of specimens, but it is ideal for storage too. See our Website. If of interest please come back to me for further information. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: 05 February 2004 18:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezer I am looking for a "small" -70 degree C. freezer for our frozen section room in the operating room. Any suggestions? Thank you. Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Hedley.Glencross <@t> CMMC.nhs.uk Fri Feb 6 03:04:06 2004 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] RE: Histonet Digest, Vol 3, Issue 6 Message-ID: Hi Sandy I have used tape coverslips in the past for conventional smears, and apart from the annoynace of having to re focus more often, and the propensity of the tape to scratch and lift off, it was acceptable. With liquid based preparations, there should be little problem with the waviness, as they should be effectively as flat as a histology section. Given the money and choice though, I would always use glass for cytology. I still think they are optically superior. I hope this helps Regards Hedley Glencross Manchester Cytology Centre UK ------------------------------ Message: 3 Date: Wed, 4 Feb 2004 13:42:34 -0800 From: "Cheasty, Sandra" Subject: [Histonet] Does Tape Coverslipper Work for Cytology Slides? To: "HistoNet \(E-mail\)" Message-ID: <2E50F33F91EEDA46A77BC3B2575BB09105875A@mars.llumc.edu> Content-Type: text/plain; charset="iso-8859-1" Hello again... The Cytopathologist here heard that the "waviness" of the tape coverslip will cause the cytologists to over-call the Pap smears. (Most of our Paps are Thin Prep) Does anyone knows of a cytology lab that uses the Sakura Tape coverslipper for paps? I would like to either confirm or dispel this rumor. Thanks, Sandy Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. ------------------------------ Message: 4 Date: Wed, 4 Feb 2004 16:49:47 -0500 From: Gary Gill Subject: RE: [Histonet] Does Tape Coverslipper Work for Cytology Slides? To: "'Cheasty, Sandra'" , "HistoNet (E-mail)" Message-ID: Content-Type: text/plain Urban legend. There are numerous technical and optical reasons that contradict using tape coverslipper, but overcalling Paps is not one of them. If the distortion were so bad, one shouldn't attempt to interpret the cells in the first place. Gary Gill From mfredrickson <@t> cohenderm.com Fri Feb 6 07:46:58 2004 From: mfredrickson <@t> cohenderm.com (Michael Fredrickson) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Availability of antibody for Rickettsia Message-ID: <3D55809F621E7C438DA80098CDE3744D10C3EB@hub.cohenderm.com> We are looking for an antibody for rickettsia (specifically Rickettsia rickettsii,) suitable for use on FFPE or FF tissue. Does anyone know of a vendor or have a protocol in place? Any information would be appreciated. Thank you. Michael Fredrickson mfredrickson@cohenderm.com From thallada <@t> noch.org Fri Feb 6 07:55:20 2004 From: thallada <@t> noch.org (Hallada, Teri) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Skin Immuno Protocols Message-ID: Does anyone out there have a Standard Skin Immuno Protocol. I'm particularly interested in protocols set up by groups to be sure that everyone practices the same procedures. Thanks, Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. From pmarcum <@t> polysciences.com Fri Feb 6 08:01:55 2004 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Xylene or "solvents" a hazard? In-Reply-To: <000901c3ec48$22c14dc0$3187080a@wsahs.nsw.gov.au> Message-ID: <000d01c3ecb9$c742bb10$4800a8c0@PMARCUM2K> Antihistamine doesn't even work for me. I can't work with limonene at all and even limited exposure results in all your symptoms and a few more we won't mention. Thanks, Pam Marcum? > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill > Sinai > Sent: Thursday, February 05, 2004 7:28 PM > To: 'peptolab'; histonet (E-mail) > Subject: RE: [Histonet] Xylene or "solvents" a hazard? > > > > Jeff, > > I agree with all you have said about the article, in my 38 years in the > laboratory 34 in Histopathology, I have never known of a female > staff member > having any troubles with birth defects, however, we have had some who have > experienced miscarriages. But they were usually staff who had not worked > for long in the laboratory and the minute we knew they were pregnant they > were moved to minimise any exposure. > > Limonene is now the bain of my life, I have become sensitised to the smell > although I have had very limited exposure over the years. An allergy test > gave results in about 15 seconds, with other related problems such as > nausea, headaches, rash, and an hayfever type reaction. The only > way I can > stop the problem is with high does of antihistamine. > > I am still not convinced that it is so wonderful. > > Bill Sinai > Laboratory Manager > Tissue Pathology, ICPMR > Westmead NSW 2145 > Australia > Ph 02 9845 7774 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of peptolab > Sent: Friday, 06 February 2004 9:42 AM > To: dellav@musc.edu > Cc: HistoNet Server > Subject: [Histonet] Xylene or "solvents" a hazard? > > > Very informative article Vinnie- but only 21 of the 125 exposed women were > histotechs- it would be more useful to know how many of the 13 major and 5 > minor malformations, the 17 fetal distress, the 9 premies, the 12 > miscarriages (not much more than unexposed- 20% greater risk) , were in > histotechs rather than the 104 persons in industry or other disciplines > presuambly using methyl ethyl ketone, benzene, and who knows what > else. This > is not to say that I don't think pregnant techs should be rotated > away from > exposure. > By the way- I read about the "horrors" of limonene- I love my limonene, > have for 20+years, and I wonder how many labs use it versus aliphatics? > Never met anyone who had a problem with it and visitors to the lab > (courriers, other techs) love the smell- when you can smell it at all. > Regards > Jeff Silverman > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________________________________________________________________ > > This electronic message and any attachments may be confidential. If you > are not the intended recipient of this message would you please delete the > message and any attachments and advise the sender. Western Sydney > Area Health Services (WSAHS) uses virus scanning software but excludes > any liability for viruses contained in any email or attachment. > > This email may contain privileged and confidential information intended > only for the use of the addressees named above. If you are not the > intended recipient of this email, you are hereby notified that any use, > dissemination, distribution, or reproduction of this email is > prohibited. If > you have received this email in error, please notify WSAHS > immediately. > > Any views expressed in this email are those of the individual sender > except where the sender expressly and with authority states them > to be the views of WSAHS. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DWilliams <@t> ciit.org Fri Feb 6 09:00:02 2004 From: DWilliams <@t> ciit.org (Delorise Williams) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] NCSHT Spring Meeting Message-ID: <12816CB59E68184F9F5F28063E68B047285D9B@xsrvr.ciit.org> The North Carolina Society of Histopathology Technologists Meeting will be held at the Hilton Wilmington Riverside in Wilmington NC on April 23-24-2004. Non Society members will need to contact me if you would like more information. Vendors, Please contact Lyle Baker at: Bakerofnc@cs.com for exhibit info. Delorise Williams CIIT Centers for Health Research PO Box 12137 Research Triangle Park, NC 27709 (919) 558-1200 Voice Mail-(919) 558-1252 Fax-(919) 558-1300 From Stephen.J.Scholz <@t> osfhealthcare.org Fri Feb 6 09:08:40 2004 From: Stephen.J.Scholz <@t> osfhealthcare.org (Scholz, Stephen J.) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Safety Message-ID: <7F1312711CA7474A89B3DF8BA0BA54D0F5F0B4@pmc-rfd-mx01.intranet.osfnet.org> Hello all; The storing of a 55 gal in the histo lab is a concern. Whether or not your CAP inspector had a problem with it is a non-factor. They probably do not know all the OSHA laws. I suggest you talk to your facilities management people about this. We went through this and there are laws ( I don't have the specifics at my finger tips) about the volume that can be stored per square foot in a manned laboratory and after a certain volume it has to be stored in a room that has an explosion out (outside) wall. It also my need to be stored with a spill containment system that handle up to the 55gal. I hope this can of worms isn't to big but it is better safe than sorry (lawsuit). Your facilities management people should be able to help you with the details. We went through it and it is a pain dealing with some of these inconvenient OSHA laws. Sorry for the info; Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 e-mail: sjscholz@osfhealthcare.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [SMTP:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, February 05, 2004 4:13 PM To: Rebecca Barnhart; Histonet (E-mail) Subject: RE: [Histonet] Safety We have a 55 gallon drum that is enclosed in a flammable cabinet. The flammable cabinet is also vented to the outside. Although we are now recycling most of our reagents, we are able to dump formalin, alcohol, and xylene all together into this one big drum and have it hauled off. It makes it very easy on us, and we have never had any problems with CAP or safety inspections. As far as fumes and smells go, we have an air filter that we got from Creative Waste Solutions, Inc. It is supposed to be good at reducing not only the smells but also the exposure to the chemicals. I use the air filter in the grossing area and several of us noticed a reduction in the formalin fumes. It is also supposed to work with xylene. I am not affilitate with Creative Waste and if you're interested, their number is (888) 795-8300. The people there are great to work with. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Thursday, February 05, 2004 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adelaur <@t> nimr.mrc.ac.uk Thu Feb 5 21:08:10 2004 From: adelaur <@t> nimr.mrc.ac.uk (April DeLaurier) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Fluorophore soluble in BABB Message-ID: Hi everyone, Does anyone have any experience with using fluorescent-labelled antibodies with a fluorophore that is NOT soluble in clearing agent benzyl alcohol/benzyl benzoate (BABB)? I have had success with Texas Red , but have found the following soluble: FITC, GFP, Alexa 488, Cy2. Any tips?? Thanks very much. April -- April DeLaurier, PhD Division of Developmental Biology National Institute for Medical Research The Ridgeway, Mill Hill London NW7 1AA United Kingdom Tel: +44 208 959 3666 ext. 2095 Fax: +44 208 816 4477 E-mail: adelaur@nimr.mrc.ac.uk From gcallis <@t> montana.edu Fri Feb 6 10:20:09 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Fluorophore soluble in BABB In-Reply-To: Message-ID: <3.0.6.32.20040206092009.00be47a8@gemini.msu.montana.edu> We do not dehydrate and clear any IFA stained sections, they are mounted with a suitable aqueous mounting media with some using Molecular Probes mounting medias (there are two) that are superb for many fluorophores. At 03:08 AM 2/6/2004 +0000, you wrote: >Hi everyone, > >Does anyone have any experience with using fluorescent-labelled >antibodies with a fluorophore that is NOT soluble in clearing agent >benzyl alcohol/benzyl benzoate (BABB)? I have had success with Texas >Red , but have found the following soluble: FITC, GFP, Alexa 488, Cy2. > >Any tips?? > >Thanks very much. >April > >-- >April DeLaurier, PhD >Division of Developmental Biology >National Institute for Medical Research >The Ridgeway, Mill Hill >London NW7 1AA >United Kingdom >Tel: +44 208 959 3666 ext. 2095 >Fax: +44 208 816 4477 >E-mail: adelaur@nimr.mrc.ac.uk > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From tpmorken <@t> labvision.com Fri Feb 6 10:36:08 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:32 2005 Subject: Flammables and CAP RE: [Histonet] Safety Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA203582F@usca0082k08.labvision.apogent.com> Laurie wrote: <> Here is the pertinent section of the July 2003 CAP checklist (Applies to CAP-accredited labs only, a US agency) having to do with flammable storage. Further below are all the flammable checklist items. Flammable storage *************************************************************** GEN.72050 Phase II N/A YES NO Are supplies of flammable and combustible liquids reasonable for the laboratory's needs, and are they properly stored? NOTE: In each laboratory area, up to 1 gallon of Class I, II, and IIIA liquids may be stored on open shelving for each 100 ft2 of space defined by fire-resistant walls/doors. Up to 2 gallons of Class I, II, and IIIA liquids may be stored in safety cans and safety cabinets for each 100 ft2. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). COMMENTARY: The supplies of flammable liquids were excessive or they were improperly stored. No more than 1 gallon of class I, II, and IIIA liquids may be stored on open shelving per 100 ft2 of space defined by fire-resistant walls/doors. No more than 2 gallons of class I, II, and IIIA liquids may be stored in safety cans and safety cabinets per 100 ft2 in any laboratory work area. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). REFERENCES: 1) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996; 2) National Fire Protection Association. Standard 99, chapter 10-7.2.2. Quincy, MA: NFPA, 2000. ***************************************************************** Can anyone say their lab meets this standard? It allows only a very small amount of flammables to be in the working lab area. Lets say you had a 55 gallon drum of flammables in your lab (where people are working) waiting to be carted off, or even recycled. If it is in a flammable cabinet and you have sprinklers you need a minimum of over 1300 square feet of lab space just for that (55/4 * 100). If you have more flammables you need even more space. This holds for any flammables in an area where people are working - for instance, those stored for use in processors, stainers etc. Not to mention those already in the processors and stainers. The only real solution to this is to have a dedicated storage area for bulk flammables that is isolated from the lab. Preferably one that is designed to contain/suppress a big fire. We had the "bunker" at one hosptial I worked at - an underground storage area with spark-proof electrical system and a bomb-proof door that was used for all bulk flamables storage. BUT, having said that, I have to say that in 8 CAP inspections I never had a CAP inspector even comment on this, despite being over the standard in almost every case. So maybe in the real world nobody really cares about it. Tim Morken All the flammable standards **************************************************************************** ********* GEN.72000 Phase I N/A YES NO Are safety cans used instead of glass bottles for volumes of flammable solvents larger than one quart (or larger than one pint for solvents that are highly volatile such as isopentane) if the purity required does not mandate glass storage? COMMENTARY: Safety cans should be used for bulk storage of flammable and combustible liquid (National Fire Protection Association classes I and II). Metal or DOT approved plastic containers provide an intermediate level of hazard containment between glass and safety cans. One pint of a highly volatile solvent, such as isopentane, stored in glass has about the same ignitability risk as 2 gallons stored in safety cans. Safety cans should be used instead of glass bottles if the purity required does not mandate glass storage. REFERENCE: National Fire Protection Association. Flammable and combustible liquids code. Standard 30, chapter 10-2.2.4.3. Quincy, MA: NFPA, 1996. **************************************************************************** ************** GEN.72050 Phase II N/A YES NO Are supplies of flammable and combustible liquids reasonable for the laboratory's needs, and are they properly stored? NOTE: In each laboratory area, up to 1 gallon of Class I, II, and IIIA liquids may be stored on open shelving for each 100 ft2 of space defined by fire-resistant walls/doors. Up to 2 gallons of Class I, II, and IIIA liquids may be stored in safety cans and safety cabinets for each 100 ft2. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). COMMENTARY: The supplies of flammable liquids were excessive or they were improperly stored. No more than 1 gallon of class I, II, and IIIA liquids may be stored on open shelving per 100 ft2 of space defined by fire-resistant walls/doors. No more than 2 gallons of class I, II, and IIIA liquids may be stored in safety cans and safety cabinets per 100 ft2 in any laboratory work area. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). REFERENCES: 1) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996; 2) National Fire Protection Association. Standard 99, chapter 10-7.2.2. Quincy, MA: NFPA, 2000. **************************************************************************** *************** GEN.72100 Phase II N/A YES NO Are storage areas and/or rooms where volatile solvents are used adequately ventilated? COMMENTARY: Areas where flammable liquids are used must be ventilated for protection of employee health, as well as fire prevention. Areas where flammable liquids are stored should be ventilated primarily for fire protection. Storage cabinets do not need to be vented, but if they are vented the duct system must be explosion proof. REFERENCE: National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996. **************************************************************************** *************** GEN.72150 Phase II N/A YES NO Are flammable or combustible liquids or gas cylinders positioned well away from open flame or other heat sources, not in corridors and not within exhaust canopies? COMMENTARY: Flammable or combustible liquids or gas cylinders must not be positioned near open flame, heat sources, in corridors, or within exhaust canopies. REFERENCE: National Fire Protection Association. Standard 99, chapter 10-7.2.4. Quincy, MA: NFPA, 2000. **REVISED** 07/31/2003 **************************************************************************** **************** GEN.72200 Phase I N/A YES NO If flammables are decanted from large drum (bulk) containers, is the secondary (receiving) container grounded? COMMENTARY: Transfer of flammable liquid from bulk storage containers should be made in storage rooms as described in NFPA 30, and the secondary container(s) should be grounded to prevent static electrical discharge if constructed of metal or other conductive material. REFERENCES: 1) National Fire Protection Association. Recommended practice on static electricity. Standard 77. Quincy, MA: NFPA, 1993; 2) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996. -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Thursday, February 05, 2004 2:13 PM To: Rebecca Barnhart; Histonet (E-mail) Subject: RE: [Histonet] Safety We have a 55 gallon drum that is enclosed in a flammable cabinet. The flammable cabinet is also vented to the outside. Although we are now recycling most of our reagents, we are able to dump formalin, alcohol, and xylene all together into this one big drum and have it hauled off. It makes it very easy on us, and we have never had any problems with CAP or safety inspections. As far as fumes and smells go, we have an air filter that we got from Creative Waste Solutions, Inc. It is supposed to be good at reducing not only the smells but also the exposure to the chemicals. I use the air filter in the grossing area and several of us noticed a reduction in the formalin fumes. It is also supposed to work with xylene. I am not affilitate with Creative Waste and if you're interested, their number is (888) 795-8300. The people there are great to work with. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Thursday, February 05, 2004 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From p_bourne_14526 <@t> yahoo.com Fri Feb 6 10:40:33 2004 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] cassette printers Message-ID: <20040206164033.83778.qmail@web10010.mail.yahoo.com> Dear all, We are looking to purchase a new cassette printer for our grossing area. What is everyone using? Does is have the ability to be directly linked to the LIS? Are you required to buy cassettes from that manufacture? How many cassettes does it hold? Thanks upfront for the information. Pat --------------------------------- Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online From ritta69 <@t> iwon.com Fri Feb 6 10:55:42 2004 From: ritta69 <@t> iwon.com (ritta69@iwon.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Golgi-Cox & aqueous mounting media Message-ID: <20040206165542.B718627C90@email.iwon.com> I am trying to get the golgi-cox stain working in rat neonatal brain tissue. I am using perfusion fixed tissue (10% formalin) and then immersing in impregnation fluid. I am using mixture taken from Ramon-Moliner's 1970 paper in contemporary methods in neuroanatomy. I do not have access to the equipment used for plastic or celloidin embedding and thus, am sectioning the tissue on a freezing microtome after washing and cryoprotecting in 30%sucrose. I am finding that it is necessary to cut sections at least 180um thick to get decent sections. Otherwise, the sections fold upon themselves and are not unfolding. So, is this what most people have found trying to cut frozen sections that were treated with the Golgi-Cox impregnation fluid? And what do I do to coverslip these thick sections? What is Canadian Balsam? Also, I have been using hardset from vector to coverslip slides from a seperate experiment that were stained with fluorojade. So far I don't like this aqueous mounting media. It seems to thick and bubbles form far to easily. Can I dilute it with PBS? I used to use Gel-mount which I liked better. Brad Drexel U of Med School _______________________________________________ From northma <@t> ohsu.edu Fri Feb 6 11:10:16 2004 From: northma <@t> ohsu.edu (Mary North) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Neurofilament protein Message-ID: Is anyone using a low molecular weight NFP for FFPE slides that does not require heat-induced antigen retrieval? The sections of peripheral nerve on charged slides with overnight warming followed by oven heat are not staying on the slide after steamer/citrate retrieval. Is there another type of slide or adhesive which helps? Any information on these issues is welcomed and appreciated. Mary North, HT(ASCP), HTL Oregon Health and Science University Portland, OR From ldunikoski <@t> rbmc.org Fri Feb 6 11:15:10 2004 From: ldunikoski <@t> rbmc.org (Dunikoski, Leonard PhD) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Histotech Position Available Message-ID: <7A2DF880A7A1D511A63300508BF7D5550271AE43@es1.rbmc.org> Raritan Bay Medical Center has an immediate opening for a full-time histotechnologist at its Perth Amboy, NJ hospital location. This is a Monday to Friday day shift opening in a CAP-accredited, computerized (CoPath) laboratory that serves both the medical center and the county Medical Examiner's Office. IHC experience a plus. We take "pride in caring" for our patients and our staff, and we offer an excellent work environment (in a safety-conscious, well-ventilated lab). If you're interested in joining our team, please email your curriculum vitae to: Leonard K. Dunikoski, Ph.D., DABCC Director of Operations Raritan Bay Medical Center ldunikoski@rbmc.org NOTICE: The information included in this email contains confidential information belonging to the sender. This information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents hereof is strictly prohibited. If you have received this email in error, please notify the sender immediately. From juan.gutierrez <@t> christushealth.org Fri Feb 6 11:41:00 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Neurofilament protein Message-ID: I think you need to work on your slide problem before switching antibodies. Do you use coated slides? If you do, do you put any type of adhesive in your water bath(no-no)? We use coated slides that we get from Stat-Lab. They are German-made and of very good quality. We used Erie slides before(Erie supplies a lot of vendors with their slides), but found them to be unreliable. We had the same problem with tissue falling off. Surgipath also makes their own slides and they were good for a while, but they also started having some quality problems. Moral of the story, ask for samples and see what works best for you. When it comes to the waterbath, if you are using coated slides there is no need to put gelatin or Stay-on or glue or what have you in there. All the additive does is neutralize the charge on the slide and not letting your tissue adhere properly to it. We use charged slides for everything, including H&E's, so we dont add anythig at all to our water. Hope this helps and good luck, Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Mary North [mailto:northma@ohsu.edu] Sent: Fri 2/6/2004 11:10 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Neurofilament protein Is anyone using a low molecular weight NFP for FFPE slides that does not require heat-induced antigen retrieval? The sections of peripheral nerve on charged slides with overnight warming followed by oven heat are not staying on the slide after steamer/citrate retrieval. Is there another type of slide or adhesive which helps? Any information on these issues is welcomed and appreciated. Mary North, HT(ASCP), HTL Oregon Health and Science University Portland, OR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Feb 6 11:44:12 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:32 2005 Subject: Flammables and CAP RE: [Histonet] Safety Message-ID: The key in this is "space defined by fire resistant doors/walls". Because the *firewall* of our lab is not our labs wall (confused?) but the wall of the metabolic lab down the hall we can count the extra space in our footage. If your lab does not have firewalls or only one firewall and the other is in another lab....you get to count all the space in your footage. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Friday, February 06, 2004 10:36 AM To: Histonet (E-mail) Subject: Flammables and CAP RE: [Histonet] Safety Laurie wrote: <> Here is the pertinent section of the July 2003 CAP checklist (Applies to CAP-accredited labs only, a US agency) having to do with flammable storage. Further below are all the flammable checklist items. Flammable storage *************************************************************** GEN.72050 Phase II N/A YES NO Are supplies of flammable and combustible liquids reasonable for the laboratory's needs, and are they properly stored? NOTE: In each laboratory area, up to 1 gallon of Class I, II, and IIIA liquids may be stored on open shelving for each 100 ft2 of space defined by fire-resistant walls/doors. Up to 2 gallons of Class I, II, and IIIA liquids may be stored in safety cans and safety cabinets for each 100 ft2. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). COMMENTARY: The supplies of flammable liquids were excessive or they were improperly stored. No more than 1 gallon of class I, II, and IIIA liquids may be stored on open shelving per 100 ft2 of space defined by fire-resistant walls/doors. No more than 2 gallons of class I, II, and IIIA liquids may be stored in safety cans and safety cabinets per 100 ft2 in any laboratory work area. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). REFERENCES: 1) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996; 2) National Fire Protection Association. Standard 99, chapter 10-7.2.2. Quincy, MA: NFPA, 2000. ***************************************************************** Can anyone say their lab meets this standard? It allows only a very small amount of flammables to be in the working lab area. Lets say you had a 55 gallon drum of flammables in your lab (where people are working) waiting to be carted off, or even recycled. If it is in a flammable cabinet and you have sprinklers you need a minimum of over 1300 square feet of lab space just for that (55/4 * 100). If you have more flammables you need even more space. This holds for any flammables in an area where people are working - for instance, those stored for use in processors, stainers etc. Not to mention those already in the processors and stainers. The only real solution to this is to have a dedicated storage area for bulk flammables that is isolated from the lab. Preferably one that is designed to contain/suppress a big fire. We had the "bunker" at one hosptial I worked at - an underground storage area with spark-proof electrical system and a bomb-proof door that was used for all bulk flamables storage. BUT, having said that, I have to say that in 8 CAP inspections I never had a CAP inspector even comment on this, despite being over the standard in almost every case. So maybe in the real world nobody really cares about it. Tim Morken All the flammable standards **************************************************************************** ********* GEN.72000 Phase I N/A YES NO Are safety cans used instead of glass bottles for volumes of flammable solvents larger than one quart (or larger than one pint for solvents that are highly volatile such as isopentane) if the purity required does not mandate glass storage? COMMENTARY: Safety cans should be used for bulk storage of flammable and combustible liquid (National Fire Protection Association classes I and II). Metal or DOT approved plastic containers provide an intermediate level of hazard containment between glass and safety cans. One pint of a highly volatile solvent, such as isopentane, stored in glass has about the same ignitability risk as 2 gallons stored in safety cans. Safety cans should be used instead of glass bottles if the purity required does not mandate glass storage. REFERENCE: National Fire Protection Association. Flammable and combustible liquids code. Standard 30, chapter 10-2.2.4.3. Quincy, MA: NFPA, 1996. **************************************************************************** ************** GEN.72050 Phase II N/A YES NO Are supplies of flammable and combustible liquids reasonable for the laboratory's needs, and are they properly stored? NOTE: In each laboratory area, up to 1 gallon of Class I, II, and IIIA liquids may be stored on open shelving for each 100 ft2 of space defined by fire-resistant walls/doors. Up to 2 gallons of Class I, II, and IIIA liquids may be stored in safety cans and safety cabinets for each 100 ft2. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). COMMENTARY: The supplies of flammable liquids were excessive or they were improperly stored. No more than 1 gallon of class I, II, and IIIA liquids may be stored on open shelving per 100 ft2 of space defined by fire-resistant walls/doors. No more than 2 gallons of class I, II, and IIIA liquids may be stored in safety cans and safety cabinets per 100 ft2 in any laboratory work area. These amounts may be doubled if there is an automatic fire suppression system (e.g., sprinklers). REFERENCES: 1) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996; 2) National Fire Protection Association. Standard 99, chapter 10-7.2.2. Quincy, MA: NFPA, 2000. **************************************************************************** *************** GEN.72100 Phase II N/A YES NO Are storage areas and/or rooms where volatile solvents are used adequately ventilated? COMMENTARY: Areas where flammable liquids are used must be ventilated for protection of employee health, as well as fire prevention. Areas where flammable liquids are stored should be ventilated primarily for fire protection. Storage cabinets do not need to be vented, but if they are vented the duct system must be explosion proof. REFERENCE: National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996. **************************************************************************** *************** GEN.72150 Phase II N/A YES NO Are flammable or combustible liquids or gas cylinders positioned well away from open flame or other heat sources, not in corridors and not within exhaust canopies? COMMENTARY: Flammable or combustible liquids or gas cylinders must not be positioned near open flame, heat sources, in corridors, or within exhaust canopies. REFERENCE: National Fire Protection Association. Standard 99, chapter 10-7.2.4. Quincy, MA: NFPA, 2000. **REVISED** 07/31/2003 **************************************************************************** **************** GEN.72200 Phase I N/A YES NO If flammables are decanted from large drum (bulk) containers, is the secondary (receiving) container grounded? COMMENTARY: Transfer of flammable liquid from bulk storage containers should be made in storage rooms as described in NFPA 30, and the secondary container(s) should be grounded to prevent static electrical discharge if constructed of metal or other conductive material. REFERENCES: 1) National Fire Protection Association. Recommended practice on static electricity. Standard 77. Quincy, MA: NFPA, 1993; 2) National Fire Protection Association. Flammable and combustible liquids code. Standard 30. Quincy, MA: NFPA, 1996. -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Thursday, February 05, 2004 2:13 PM To: Rebecca Barnhart; Histonet (E-mail) Subject: RE: [Histonet] Safety We have a 55 gallon drum that is enclosed in a flammable cabinet. The flammable cabinet is also vented to the outside. Although we are now recycling most of our reagents, we are able to dump formalin, alcohol, and xylene all together into this one big drum and have it hauled off. It makes it very easy on us, and we have never had any problems with CAP or safety inspections. As far as fumes and smells go, we have an air filter that we got from Creative Waste Solutions, Inc. It is supposed to be good at reducing not only the smells but also the exposure to the chemicals. I use the air filter in the grossing area and several of us noticed a reduction in the formalin fumes. It is also supposed to work with xylene. I am not affilitate with Creative Waste and if you're interested, their number is (888) 795-8300. The people there are great to work with. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Rebecca Barnhart [mailto:RBARNHART@summithealth.org] Sent: Thursday, February 05, 2004 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety After all the discussion with the pregnancy in the lab that I have read it started making me think. I have worked in the lab almost 9 years and when I started I noticed some safety concerns and finally some have began to change. Don't get me wrong, we use safety precautions but there are things we overlook because they have became the norm for us. For example we use to store our used americlear and acetone in gallon jugs (the empty alcohol, americlear, acetone, etc ones) under our stainer that sits on a wood table. There would be anywhere from 12 to 16 gallons until it was dumped, for several weeks up to a month. Finally we are getting it dumped at least once a week and in the very near future we are renovating our histo lab. This will enable us to have a 55 gallon drum in a flam cabinet so we can dump our waste in as we change the processor and stainer. Some of my concerns are dumping all waste (except americal and acetone) down the drain, cover slipping and changing the solutions. Currently we coverslip on the counter, no hood. Same with changing the solutions. I use the hood to dump old solutions out and new in but not everyone does. How does everyone else handle the issue of fumes, dumping waste down the drain and any safety issues? We are a small lab, only 2 histo tech and 1 pathologist. We are in a small room (at least with our renovation it will be opened up more, which I think will help with ventilation) All new employees seem to notice a smell, even ones that are use to the histo environment. Scary as it sounds I am use to the smell. Thanks in advance for all the input. Becky Barnhart rbarnhart@summithealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From ccrowder <@t> mail.vetmed.lsu.edu Fri Feb 6 11:49:00 2004 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Cryostats Message-ID: We and our anatomy department is looking for a new cryostat. We would like some input on the different brands. Anatomy has looked at the Brite OTF5000HS, with the 5040 microtome. It is terribly expensive. We have been looking at the Leica and Sakura. However, we haven't looked at the Thermo yet or other brands. Can any of you help us get a handle on what's out there. We will be doing all types of tissues, particularly fat and/or skin. Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 From flemons <@t> bhset.org Fri Feb 6 11:58:25 2004 From: flemons <@t> bhset.org (Fran Lemons) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Does anyone know of a quick methylene blue stain for mast cells? It rings a bell with me, but can't Message-ID: Does anyone know of a quick methylene blue stain for mast cells? It rings a bell with me, but can't put a finger on it or find it in any of our reference literature. hay-ulp!!! thanks in advance From dbpiontek <@t> hsc.wvu.edu Fri Feb 6 12:10:09 2004 From: dbpiontek <@t> hsc.wvu.edu (Denise Bland-Piontek) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Refractive index coverslipping film Message-ID: Does any one know where I may be able to order refractive index coverslipping film for glass plates? Plates are 4"x3"x1mm. I need to coverslip sections of brain for mapping purposes. Thanks, Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator West Virginia University From gentras <@t> vetmed.auburn.edu Fri Feb 6 12:10:39 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:32 2005 Subject: Fwd: [Histonet] Cryostats Message-ID: <6.0.1.1.0.20040206120925.0259d6e0@mailhost.vetmed.auburn.edu> We have a Microm 505E HM and are thoroughly pleased with it. We purchased it through Richard Alan Scientific. Best wishes. Atoska >Date: Fri, 06 Feb 2004 11:49:00 -0600 >From: "Cheryl Crowder" >To: "Histonet" >X-Mailer: WorldClient 6.8.5 >X-Authenticated-Sender: ccrowder@mail.vetmed.lsu.edu >X-MDRemoteIP: 127.0.0.1 >X-Return-Path: ccrowder@mail.vetmed.lsu.edu >X-MDaemon-Deliver-To: histonet@pathology.swmed.edu >X-Scan-Signature: ab3f4c55490e4d672805895add812bc5 >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 7bf0d7e947a05afa907d3090d45e1f8d >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] Cryostats >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes > >We and our anatomy department is looking for a new cryostat. We would >like some input on the different brands. Anatomy has looked at the >Brite OTF5000HS, with the 5040 microtome. It is terribly expensive. We >have been looking at the Leica and Sakura. However, we haven't looked >at the Thermo yet or other brands. Can any of you help us get a handle >on what's out there. We will be doing all types of tissues, >particularly fat and/or skin. Thank you, Cheryl > > >Cheryl Crowder, BA, HTL(ASCP) >Chief Technologist >Anatomic Pathology >Department of Pathobiological Sciences >School of Veterinary Medicine >Louisiana State University >Skip Bertman Drive >Baton Rouge, LA 70803 > >225-578-9734 >FAX: 225-578-9720 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From Ngilman <@t> nbhd.org Fri Feb 6 12:12:37 2004 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Job Opportunity Message-ID: Broward General Medical Center has a Full-Time - Monday - Friday 6:00am-2:30pm (hours negotiable) position for a histotechnologist: Apply on-line at www.nbhd.org Qualifications: * Florida technologist license in Histology * Must be ASCP HT/HTL or eligible * Minimum 2 years experience in Immuno Histochemistry * Minimum 2 years experience in clinical laboratory Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** From gcallis <@t> montana.edu Fri Feb 6 13:24:02 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Mast cell with toluidine blue In-Reply-To: Message-ID: <3.0.6.32.20040206122402.00bd0610@gemini.msu.montana.edu> We prefer to use a toluidine blue method that does not give any overall bluish purple background (annoying!) to the other tissue elements, just the mast cells stain. Try Churukian Schenk method from J of Histotechnology - it is a delight, easy to make up and and works on decalcified bone also. A toludine blue method for demonstration of mast cells, J Histotechnology, 4:85,1981. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From d.gregg <@t> juno.com Fri Feb 6 13:23:05 2004 From: d.gregg <@t> juno.com (d.gregg@juno.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Need good S-100 MAb for paraffin sections Message-ID: <20040206.142306.916.0.d.gregg@juno.com> Can anyone suggest a strong and robust MAb for S-100 that will work in formalin fixed or PLP fixed tissue embedded in paraffin. I have used a polyclonal rabbit antibody that works OK but I am using pig tissues and get too much background with a rabbit antibody despite various blocking regimes and adsorption of the secondary with swine serum. Thanks for any suggestions. Douglas Gregg Plum Island Animal Disease Center Greenport, NY From gentras <@t> vetmed.auburn.edu Fri Feb 6 13:42:27 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:32 2005 Subject: Fwd: [Histonet] Mast Cells Stain Message-ID: <6.0.1.1.0.20040206133709.0259c2c0@mailhost.vetmed.auburn.edu> Hello, we too use Toluidine Blue Stain (TBO) for mast cells. After deparaffinization & hydrating, stain in 0.1% Toluidine Blue Stain for 10 seconds - 1 minute depending on stain (until tissue is medium blue in color). 10 seconds works best for us. After staining rinse in distilled water, dehydrate, clear, & mount. Best wishes. Atoska >X-Mailer: Novell GroupWise Internet Agent 6.0.3 >Date: Fri, 06 Feb 2004 12:58:25 -0500 >From: "Fran Lemons" >To: >X-Scan-Signature: 1b80acdfcc20e9beac1ecc1899376e31 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 56b1ed4a6b9d2c7c11817481be952b1e >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] Does anyone know of a quick methylene blue stain for mast > cells? It rings a bell with me, but can't >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes >X-MIME-Autoconverted: from quoted-printable to 8bit by >cvm4.vetmed.auburn.edu id i16I63B24191 > >Does anyone know of a quick methylene blue stain for mast cells? It rings >a bell with me, but can't put a finger on it or find it in any of our >reference literature. hay-ulp!!! > >thanks in advance > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From Jenny-Oblander <@t> omrf.ouhsc.edu Fri Feb 6 15:58:21 2004 From: Jenny-Oblander <@t> omrf.ouhsc.edu (Jenny Oblander) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] FW: [COMPMED] Cassettes for an AO Spencer 820 rotary microtome Message-ID: I am forwarding this message for a member of the Comp Med list server. Can anyone help him ? Thanks Jenny -----Original Message----- From: Brian Gordon, DVM Sent: Friday, February 06, 2004 3:08 PM To: Jenny Oblander Subject: FW: [COMPMED] Cassetts for an AO Spencer 820 rotary microtome -----Original Message----- From: Fernando Catalan [mailto:catalanf18@yahoo.com] Sent: Friday, February 06, 2004 2:56 PM To: COMPMED@LISTSERV.AALAS.ORG Subject: [COMPMED] Cassetts for an AO Spencer 820 rotary microtome Hello, Would anyone happen to know where I can find tissue embedding cassetts for an American Optical Spencer 820 rotary microtome? These are very small cassetts with maximum dimensions of about 1.5 X 3 X 0.5 cm. Thank you in advance for any information Fernando Catalan Trinity University TX __________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html From Jacquie.Mack <@t> CLS.ab.ca Fri Feb 6 16:03:32 2004 From: Jacquie.Mack <@t> CLS.ab.ca (Jacquie.Mack@CLS.ab.ca) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] unsubscribe Message-ID: <30C050525B881C4AAFF41E6D16543E6803488A9A@mail3.cls.ab.ca> unsubscribe Jacqueline Mack Tech III , Anatomic Pathology Foothills Medical Center Calgary Laboratory Services ( (403)-944-4162 fax: (403)-270-4093 pager: 212-8223 # 0540 * Ja cquie.Mack@CLS.ab.ca Laboratory Medicine Enhancing Your Health Care From Linresearch <@t> aol.com Fri Feb 6 18:00:15 2004 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] CD Markers Message-ID: <6b.220b867b.2d55848f@aol.com> Hi, I would appreciate any hints on working-up the following Abs on FFPE rodent tissues: Cd3 Cd45R Cd21 Thanks in advance. lin From nfitzgi726 <@t> charter.net Fri Feb 6 18:59:40 2004 From: nfitzgi726 <@t> charter.net (Noreen Fitzgibbons) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Cyclin D 1 Message-ID: <00e801c3ed15$aab10590$6400a8c0@CPQ23478322552> Hello Histonet bodies, Our lab at Washoe Medical Center, in Reno Nevada has recently started doing IHC and we are having a difficult time finding either tissue for the Cyclin D 1 to make controls or finding control slides for this stain. If anyone has any information about how I can get tissue or slides for this I would be very happy. Please share. Thanks, Noreen Fitzgibbons oink oink / " \___/ " \ | ? ? | \ oo / ~~ From sonyalhogg <@t> yahoo.co.nz Sat Feb 7 01:01:45 2004 From: sonyalhogg <@t> yahoo.co.nz (=?iso-8859-1?q?Sonya=20Hogg?=) Date: Fri Sep 16 15:22:32 2005 Subject: [Histonet] Lipid Classification Message-ID: <20040207070145.69179.qmail@web14909.mail.yahoo.com> Hello, I was wondering what the current classification system is for lipids. I have seen a few various ones. Is it classified as simple, compound and derived. OR Triglycerides, Glycerophosphatides, or sphingolipids and waxes? I have found these in Sheehan and Hrapchak. They are different from the ones in Bancroft and Gamble!!!! http://greetings.yahoo.com.au - Yahoo! Greetings Send your love online with Yahoo! Greetings - FREE! From tamamakj <@t> upmc.edu Sat Feb 7 09:48:28 2004 From: tamamakj <@t> upmc.edu (Tamama, Kenichi) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] X-gal staining and fixation Message-ID: <9122C182D4268F45BCB9E64A10B7331FE8DBB7@1upmc-msx7.isdip.upmc.edu> Dear histonet members, I have been working on X-gal staining of cultured cells with iron cyanide solution to see the efficiency of adenovirus transfection, and decaying of cell structure is bothering me. I fixed cells with 1%glutaraldehyde/PBS(-) for 30 min followed with 0.2% Triton-X/PBS(-) for 10 min before incubation with iron cyanide solution. The cell structure looks fine soon after fixation, but some junks/debris of cells start floating soon after incubation with X-gal solution. Higher conc of glutaraldehyde preserves the structure more robustly, but at the same time it denatures the beta-galactosidase and loses the blue color. I would deeply appreciate your comments/suggestions/advice. Ken Tamama Kenichi Tamama, M.D.,Ph.D. Resident of Clinicial Pathology (Laboratory Medicine) University of Pittsburgh School of Medicine Department of Pathology From Raoul.Regnault <@t> interiorhealth.ca Sat Feb 7 11:12:26 2004 From: Raoul.Regnault <@t> interiorhealth.ca (Regnault, Raoul) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Technicon Ultra Manual Message-ID: <1DA1FB459D6ED44EB804DB0C8BE230951B05FF@dc1serv2> I discovered the manual for our no longer existing Ultra. Also in the binder containing the manual are 3 punched timing discs. Available for the cost of shipping to anyone having use of the manual. I would also scan and email pages of the manual for anyone wanting that. Raoul Regnault Anatomic Pathology Kootenay Boundary Regional Hospital Trail B.C. 250-368-3311 fax 364-3421 raoul.regnault@interiorhealth.ca From KHays <@t> mbhs.org Sat Feb 7 14:29:36 2004 From: KHays <@t> mbhs.org (KHays@mbhs.org) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] looking to buy a cryostat Message-ID: Kathy Tedford-Hays HT (ASCP) Technical Specialist, Histology Dept (601)-968-3070 ext 7398 Baptist Medical Center 1225 North State Street Jackson, MS 39202 looking to buy a new cryostat for our frozen room. i would like some information concerning likes and dislikes about the ones that are in use. thanks ahead of time for any information concerning this topic. The information contained in this email is confidential and is intended for the recipient only. Do not forward this email without permission from its originator. From micro <@t> formatex.org Sat Feb 7 20:10:16 2004 From: micro <@t> formatex.org (Advanced Materials) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Microsc for Cell adhesion studies Message-ID: <005501c3edea$ebe528e0$0e001aac@LocalHost> Hi, our group is studying interaction of microbial cells (yeast and bacteria) to several kinds of biomaterials. We conduct physico/chemical analysis of the involved surfaces (mainly throgh surface thermodynamical analysis: contact angles with several liquids) and have also an atomic force microscopy for high resolution mapping of the surface topography and force measurements. At this moment, we are interested in seeing what is happening with the contact side of the cell with the substratum. For this reason, we are now interested in applying optical methods to analyse cell deposition/adhesion, in order to complete a set of experiments already performed with other techniques with Candida, Enterococcus and Staphyloccocus strains. I am planning now to acquire such a type of instrument, and I am specially interested in 1. Total internal reflection microscopy 2. Confocal Laser scanning microscopy (CLSM) I would very much appreciate your advice on which of these two techniques have been proven to be more useful for this kind of experiments, and also advice on some good and cost-effective equipment (model). Of course if some of you have already experience in this kind of experiments and are open to a collaboration to get more insight into these adhesion phenomena, I will be mostly grateful if you contact me to discuss it further. Best wishes, Antonio M?ndez Vilas Interfacial Phenomena and Biosurfaces Group Department of Physics Universidad de Extremadura Avda de Elvas s/n 06001 Badajoz SPAIN E-mail: amvilas@unex.es From ekaplan <@t> squ.edu.om Sun Feb 8 00:13:37 2004 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] control tissues Message-ID: Good morning, I realise this is a subject which has been previously discussed but I wonder if anyone has had experience of 'manufacturing' their own control blocks? I have a student about to start a project and would apreciate any protocols. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman From Rcartun <@t> harthosp.org Sun Feb 8 06:29:49 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Cyclin D 1 Message-ID: If you are using cyclin D1 to confirm a diagnosis of mantle cell lymphoma, you need to use a mantle cell lymphoma that overexpresses this nuclear protein as a positive control. Richard Cartun >>> "Noreen Fitzgibbons" 02/06/04 07:59PM >>> Hello Histonet bodies, Our lab at Washoe Medical Center, in Reno Nevada has recently started doing IHC and we are having a difficult time finding either tissue for the Cyclin D 1 to make controls or finding control slides for this stain. If anyone has any information about how I can get tissue or slides for this I would be very happy. Please share. Thanks, Noreen Fitzgibbons oink oink / " \___/ " \ | ? ? | \ oo / ~~ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Feb 8 09:29:10 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] CPT coding situation Message-ID: For those of you familiar with CPT coding for surgical pathology, I would like to confirm my interpretation of the following situation: The specimen is a "Left breast, partial mastectomy" and the pathologist found and diagnosed two separate invasive duct carcinomas (tissue blocks 2B and 2K). Ancillary studies (ER, PR, and c-erbB-2) were performed on the two separate tumors. I charged for one set of IHC studies (block 2B), but "no charged" the second set (2K) since both studies were performed on blocks from the same specimen. Is this correct? Thank you. Richard Cartun From aiwaniuk <@t> ualberta.ca Sun Feb 8 16:10:32 2004 From: aiwaniuk <@t> ualberta.ca (Andrew Iwaniuk) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] museum specimens Message-ID: <4028187B@webmail.ualberta.ca> Hello all, I have a question regarding the preparation of museum specimens for histology. I recently obtained access to several museum specimens (formalin fixed and then preserved in 70% ethanol) that we are using for a comparative neuroanatomical study. We are hoping to successfully extract the brains and section the tissue to examine cerebellar structure. I was thinking of taking the brains through a graded series of ethanols to PBS, post-fixing in PFA and gelatin embedding them for frozen sections. Alternatives would be to leave it in 70% ethanol and section it on a vibratome or paraffin embed them. Does anyone have any experience in performing histology on museum specimens like these or suggestions/advice on the matter? Thanks, Andrew Iwaniuk Andrew N. Iwaniuk Post-doctoral Research Fellow Songbird Neuroethology Lab Department of Psychology University of Alberta P217 Biological Sciences Building Edmonton, Alberta, T6G 2E9 Canada Ph: +1 780 492 0323 Fax: +1 780 492 1768 From billions <@t> public1.sz.js.cn Sun Feb 8 18:25:18 2004 From: billions <@t> public1.sz.js.cn (Sinoera Tech) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Re: Alcian Yellow Alcian Blue References: <4028187B@webmail.ualberta.ca> Message-ID: <004701c3eea3$344dcde0$a001a8c0@ming> Dear Sirs, We are one research & development based custom manufacturer in China We can supply quality quality Alcian Blue, Alcian Yellow and Alcian Green in the long run. Please contact us for the dyes. Kind Regards. - Minggeng Wang, Ph.D / President SUZHOU SINOERA CHEM CO., LTD. No.5508, 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68224995 Tel: +86 512 68246939 From Monjes <@t> aol.com Sun Feb 8 20:25:53 2004 From: Monjes <@t> aol.com (Monjes@aol.com) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] museum specimens Message-ID: <158.2d2a7921.2d5849b1@aol.com> A cytotechnologist I have worked with for 20 years has been using A portion of lung which contains lung cancer for teaching purposes,he uses 50% alcohol. And changes alcohol yearly. From Marjorie.Lehman <@t> unilever.com Mon Feb 9 06:39:08 2004 From: Marjorie.Lehman <@t> unilever.com (marjorie lehman) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Skin Immuno Protocols Message-ID: Please post them to Histonet too. Thanks. -----Original Message----- From: Hallada, Teri [SMTP:thallada@noch.org] Sent: Friday, February 06, 2004 8:55 AM To: Histonet Subject: [Histonet] Skin Immuno Protocols Does anyone out there have a Standard Skin Immuno Protocol. I'm particularly interested in protocols set up by groups to be sure that everyone practices the same procedures. Thanks, Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From otis2k <@t> yahoo.com Mon Feb 9 07:51:37 2004 From: otis2k <@t> yahoo.com (tony filips) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Microwave decalcification Message-ID: <20040209135137.75737.qmail@web20603.mail.yahoo.com> Our lab is investigating microwave decal (with/without vacuum). Can anyone direct me to some m-wave procedures. Thanks... Anthony Filipunas Histology Manager MPI Research Mattawan, MI email- otis2k@yahoo.com __________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html From piernoel <@t> health.nb.ca Mon Feb 9 07:58:00 2004 From: piernoel <@t> health.nb.ca (Pierre Noel) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Digital Photography in Pathology Message-ID: <402791E7.A46BBA96@health.nb.ca> Hi everyone! We are a mid size pathology lab looking to purchase digital photographic equipment for general use in specimen and autopsy pictures. Please reply on the best systems available from personal experience. Also, how do you process, store and print your pictures. Thank you! From funderwood <@t> mcohio.org Mon Feb 9 08:17:01 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Does anyone know of a quick methylene blue stain for mastcells? It rings a bell with me, Message-ID: I use a 0.5% toluidine blue in 0.5N HCL. Stain for 30 minutes then rapidly dehydrate in 3 changes of absolute alcohol, clear and coverslip. Granules stain a nice purplish color. Fred >>> "Fran Lemons" 02/06/04 12:58PM >>> Does anyone know of a quick methylene blue stain for mast cells? It rings a bell with me, but can't put a finger on it or find it in any of our reference literature. hay-ulp!!! thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From StarkusL <@t> ummhc.org Mon Feb 9 08:47:58 2004 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Digital Photography in Pathology Message-ID: Do you want microscopic photography or general photography? -----Original Message----- From: piernoel@health.nb.ca [mailto:piernoel@health.nb.ca] Sent: Monday, February 09, 2004 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital Photography in Pathology Hi everyone! We are a mid size pathology lab looking to purchase digital photographic equipment for general use in specimen and autopsy pictures. Please reply on the best systems available from personal experience. Also, how do you process, store and print your pictures. Thank you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Mon Feb 9 08:40:45 2004 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Control probes for chick and mouse embryos and adult tissues Message-ID: We are currently doing ISH techniques for the researchers here and as a matter of quality control, we would like to find a probe or probes that can be used as a positive control in mouse and chick tissues. We'd prefer not to use regularly housekeeping genes because all the tissue elements will stain and that would be difficult to distinguish any sensitivity or specificity issues. What do you use in this situation? Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From cwscouten <@t> myneurolab.com Mon Feb 9 09:13:37 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] museum specimens Message-ID: Depends on how thick you want the specimen sections to be. With a Vibratome, you can cut brain that hardened to 20 micron (if care is used) or thicker sections. Thinner sections requires freezing or embedding. Cells might be in better shape with vibratome sectioning than if you freeze or embed. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com ________________________________ -----Original Message----- From: Andrew Iwaniuk [mailto:aiwaniuk@ualberta.ca] Sent: 08 February 2004 22:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] museum specimens Hello all, I have a question regarding the preparation of museum specimens for histology. I recently obtained access to several museum specimens (formalin fixed and then preserved in 70% ethanol) that we are using for a comparative neuroanatomical study. We are hoping to successfully extract the brains and section the tissue to examine cerebellar structure. I was thinking of taking the brains through a graded series of ethanols to PBS, post-fixing in PFA and gelatin embedding them for frozen sections. Alternatives would be to leave it in 70% ethanol and section it on a vibratome or paraffin embed them. Does anyone have any experience in performing histology on museum specimens like these or suggestions/advice on the matter? Thanks, Andrew Iwaniuk Andrew N. Iwaniuk Post-doctoral Research Fellow Songbird Neuroethology Lab Department of Psychology University of Alberta P217 Biological Sciences Building Edmonton, Alberta, T6G 2E9 Canada Ph: +1 780 492 0323 Fax: +1 780 492 1768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Mon Feb 9 09:06:08 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] CD Markers Message-ID: Dako Cd3 code A0452 work well in mice pretreat with 0.1mM Citrate pH 6.0 Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Linresearch@aol.com [mailto:Linresearch@aol.com] Sent: Friday, February 06, 2004 5:00 PM To: histonet@pathology.swmed.edu Subject: [Histonet] CD Markers Hi, I would appreciate any hints on working-up the following Abs on FFPE rodent tissues: Cd3 Cd45R Cd21 Thanks in advance. lin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cormier <@t> MIT.EDU Mon Feb 9 08:50:47 2004 From: cormier <@t> MIT.EDU (Kathleen Cormier) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Nikon inverted 'scope power supply Message-ID: <5.0.2.1.2.20040209094348.00ac4fe0@hesiod> Hello all, We have a Nikon inverted 'scope an "Eclipse TE 300" that we need a power supply for. (TE-PS100/TE-PSE100 power supply) As the story goes the power supply was lost during storage, and is no where to be found. The power supply is no longer made (!!) by Nikon ( how can this be true?) Is there anyone out there willing to sell (cheaply) or trade for this power supply? We have some real sweet Helicobacter control blocks that might prove to be useful to someone....... Kathy Cormier Histology/Necropsy Supervisor DCM-MIT From donna <@t> phxbio.com Mon Feb 9 09:28:57 2004 From: donna <@t> phxbio.com (Donna Brown) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Control probes for chick and mouse embryos and adult tissues In-Reply-To: References: Message-ID: <6.0.1.1.0.20040209091440.027db230@phxbio.com> Hi Teri- When working with ISH probes to pick up RNA transcripts (vs. DNA sequences) we like to use a poly T probe as the vast majority of mRNA contain a poly A tail. The probe should light up every cell and works quite nicely to check if the pretreatment and hybridization steps make all the cells available for probe hybridization. Our company, Phoenix BioTechnologies (the old Research Genetics crew) sells a poly T probe prelabeled with a tail containing multiple biotins, making the control probe step fast and easy. You can view our in stock probes at http://www.phxbio.com/products-probes.php3 and other solutions, including our popular Stable DAB at http://www.phxbio.com/products-solutions.php3 If you'd like further info about controls for probing DNA targets, please contact me. Best regards- Donna Donna Brown Phoenix BioTechnologies 1000 Meridain St Huntsville, AL 35801 (866) 319-0900 phone (256) 319-0902 fax donna@phxbio.com http://phxbio.com >We are currently doing ISH techniques for the researchers here and as a >matter of quality control, we would like to find a probe or probes that >can be used as a positive control in mouse and chick tissues. We'd >prefer not to use regularly housekeeping genes because all the tissue >elements will stain and that would be difficult to distinguish any >sensitivity or specificity issues. What do you use in this situation? > > >Teri Johnson >Managing Director Histology Facility >Stowers Institute for Medical Research >1000 E. 50th St. >Kansas City, Missouri 64110 >tjj@stowers-institute.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sa.drew <@t> hosp.wisc.edu Mon Feb 9 07:40:20 2004 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] CPT coding situation Message-ID: Unfortunately, that would be our approach too, sigh. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Sunday, February 08, 2004 9:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding situation For those of you familiar with CPT coding for surgical pathology, I would like to confirm my interpretation of the following situation: The specimen is a "Left breast, partial mastectomy" and the pathologist found and diagnosed two separate invasive duct carcinomas (tissue blocks 2B and 2K). Ancillary studies (ER, PR, and c-erbB-2) were performed on the two separate tumors. I charged for one set of IHC studies (block 2B), but "no charged" the second set (2K) since both studies were performed on blocks from the same specimen. Is this correct? Thank you. Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Feb 9 12:55:06 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] museum specimens In-Reply-To: <4028187B@webmail.ualberta.ca> References: <4028187B@webmail.ualberta.ca> Message-ID: <4027D78A.4030908@umdnj.edu> Hi Andrew: I don't know if 'refixing' in PFA will do much to tissue that has already seen formalin and 70% ethanol for extended periods of time. The 'best' way to proceed probably depends on how thick you want the sections to be for the microscopic method you want to use. Paraffin embedding will allow for the thinest sections, less than 20 microns. Frozen/vibratome for thicker sections (20-50 microns), perhaps nitrocellulose for really thick (100 micron) sections for 3D reconstruction of Purkinje cells? I would try one method on one or two brains to see how it works before committing all of the tissue to one fate. Geoff Andrew Iwaniuk wrote: >Hello all, > >I have a question regarding the preparation of museum specimens for histology. > I recently obtained access to several museum specimens (formalin fixed and >then preserved in 70% ethanol) that we are using for a comparative >neuroanatomical study. We are hoping to successfully extract the brains and >section the tissue to examine cerebellar structure. I was thinking of taking >the brains through a graded series of ethanols to PBS, post-fixing in PFA and >gelatin embedding them for frozen sections. Alternatives would be to leave it >in 70% ethanol and section it on a vibratome or paraffin embed them. > >Does anyone have any experience in performing histology on museum specimens >like these or suggestions/advice on the matter? > >Thanks, >Andrew Iwaniuk > >Andrew N. Iwaniuk >Post-doctoral Research Fellow >Songbird Neuroethology Lab >Department of Psychology >University of Alberta >P217 Biological Sciences Building >Edmonton, Alberta, T6G 2E9 >Canada >Ph: +1 780 492 0323 >Fax: +1 780 492 1768 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Don.Birgerson <@t> leica-microsystems.com Mon Feb 9 10:32:28 2004 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] FW: [COMPMED] Cassettes for an AO Spencer 820 rotary microtome Message-ID: Hi Jenny, Have Fernando give me a call. His message is confusing from an AO point of view. I have evolved into the Leica Co from AO, Reichert , and Cambridge side of the cooperate family and think he might be talking about embedding rings. Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 Jenny Oblander > cc: Sent by: Subject: [Histonet] FW: [COMPMED] Cassettes for an AO Spencer 820 rotary microtome histonet-bounces@lists.utsouth western.edu 02/06/2004 03:58 PM I am forwarding this message for a member of the Comp Med list server. Can anyone help him ? Thanks Jenny -----Original Message----- From: Brian Gordon, DVM Sent: Friday, February 06, 2004 3:08 PM To: Jenny Oblander Subject: FW: [COMPMED] Cassetts for an AO Spencer 820 rotary microtome -----Original Message----- From: Fernando Catalan [mailto:catalanf18@yahoo.com] Sent: Friday, February 06, 2004 2:56 PM To: COMPMED@LISTSERV.AALAS.ORG Subject: [COMPMED] Cassetts for an AO Spencer 820 rotary microtome Hello, Would anyone happen to know where I can find tissue embedding cassetts for an American Optical Spencer 820 rotary microtome? These are very small cassetts with maximum dimensions of about 1.5 X 3 X 0.5 cm. Thank you in advance for any information Fernando Catalan Trinity University TX __________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ From StarkusL <@t> ummhc.org Mon Feb 9 10:18:08 2004 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Digital Photography in Pathology Message-ID: For general photography, we use an Olympus C-3000 zoom. It's a great camera and fairly "idiot proof". Pictures are stored on a hard drive and searchable by database (of my design). Computer is backed up each night onto tape so if the computer crashes we have backup. Let me know if you are interested in looking at the database for pictures. It works well and is also fairly "idiot proof". -----Original Message----- From: piernoel@health.nb.ca [mailto:piernoel@health.nb.ca] Sent: Monday, February 09, 2004 11:11 AM To: Starkus, Laurie Subject: Re: [Histonet] Digital Photography in Pathology Sorry! I should have specified, I looking at general photography. Thank you! "Starkus, Laurie" wrote: > Do you want microscopic photography or general photography? > > -----Original Message----- > From: piernoel@health.nb.ca [mailto:piernoel@health.nb.ca] > Sent: Monday, February 09, 2004 8:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Digital Photography in Pathology > > Hi everyone! > We are a mid size pathology lab looking to purchase digital > photographic equipment for general use in specimen and autopsy pictures. > Please reply on the best systems available from personal experience. > Also, how do you process, store and print your pictures. > > Thank you! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From siksik03 <@t> comcast.net Mon Feb 9 10:29:26 2004 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Microwave decalcification In-Reply-To: <20040209135137.75737.qmail@web20603.mail.yahoo.com> References: <20040209135137.75737.qmail@web20603.mail.yahoo.com> Message-ID: Hi HistoNetters Tony Filips asked about microwave decalcification procedures. A laboratory microwave with a vented cavity (for fumes) and temperature control is required for this application. References can be found in Kok & Boon, chapter 16, pp 226-229. In general, I recommend use of a 10% formic acid solution, although 5% formic acid /5 % hydrochloric acid can be used where immunohistochemistry will not be as much of an issue. EDTA can also be used. I use a temperature of 50?C for trephine biopsies and 37?C for larger bone samples in the Milestone microwaves. Some form of agitation is very helpful to keep fresh decalcification fluid always in contact with the specimen. It is critical that proper fixation be caried out, on the bench or in the microwave, prior to decalcification. best regards, Steven Slap Microwave Consultant At 5:51 AM -0800 2/9/04, tony filips wrote: >Our lab is investigating microwave decal (with/without >vacuum). Can anyone direct me to some m-wave >procedures. Thanks... >Anthony Filipunas >Histology Manager >MPI Research >Mattawan, MI > >email- otis2k@yahoo.com > >__________________________________ >Do you Yahoo!? >Yahoo! Finance: Get your refund fast by filing online. >http://taxes.yahoo.com/filing.html > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Mon Feb 9 10:51:30 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] FW: [IHCRG] ck7 and ck20 positive tumors Message-ID: -----Original Message----- From: Patsy Ruegg [mailto:pruegg@msn.com] Sent: Monday, February 09, 2004 9:33 AM To: ihcrg@yahoogroups.com Subject: [IHCRG] ck7 and ck20 positive tumors Dear Friends, Many of those attempting the QIHC exam this go round are in despirate need of tumors positive for cytokeratin 7 and cytokeratin 20. If you can help us obtain any of this tissue for testing we would really appreciate it. I can provide a fed.x number for shippment of tissues to the address listed below. Best regards, Patsy, Chair NSH IHC Resource Group Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 216 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 HP-303-644-4538 hm email pruegg@msn.com wk email pruegg@colobio.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _________________________________________________________________ Get some great ideas here for your sweetheart on Valentine's Day - and beyond. http://special.msn.com/network/celebrateromance.armx ------------------------ Yahoo! Groups Sponsor ---------------------~--> Buy Ink Cartridges or Refill Kits for your HP, Epson, Canon or Lexmark Printer at MyInks.com. Free s/h on orders $50 or more to the US & Canada. http://www.c1tracking.com/l.asp?cid=5511 http://us.click.yahoo.com/mOAaAA/3exGAA/qnsNAA/asSolB/TM ---------------------------------------------------------------------~-> Yahoo! Groups Links <*> To visit your group on the web, go to: http://groups.yahoo.com/group/IHCRG/ <*> To unsubscribe from this group, send an email to: IHCRG-unsubscribe@yahoogroups.com <*> Your use of Yahoo! Groups is subject to: http://docs.yahoo.com/info/terms/ From RFail <@t> Charleston.net Mon Feb 9 10:50:40 2004 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] FW: Buffer/Alkaline phosphatase stainng sytems Message-ID: <001f01c3ef2c$da371bb0$db11a6a5@rena> -----Original Message----- We perform IHC on the DAKO Autostainer, at present we am using a commercial concentrate of TRIS buffer with Tween . We are considering making our buffers. I have been told that phosphate buffers are not compatible with alkaline phosphatase staining systems. Does anyone know of any references to buffer/alkaline phophatase incompatibility? Thank you Rena Fail From syedab <@t> totalise.co.uk Mon Feb 9 10:18:56 2004 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Dako ChemMate Envision kit Message-ID: <004a01c3ef28$6b07a7e0$80c401a3@clneuro.ox.ac.uk> Hi all, I have been using the Dako Envision ChemMate kit for three weeks with no success. I wondered whether I was doing something obviously silly like using the wrong pH. In the protocol it mentions to use the Dako buffer kit. Could someone tell me whether it suddenly needs to change pH half way through the protocol? I am using PBS at pH 7.4 made up from tablets. Thanks in advance Anila Syed --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.580 / Virus Database: 367 - Release Date: 06/02/04 From juan.gutierrez <@t> christushealth.org Mon Feb 9 11:11:17 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Cryostats Message-ID: Just stating facts here. I've used a Microm with the vacutome and it was a dream to cut in it. There was lots of room for specimens inside and for the most part it was pretty reliable. Now I'm in a different lab with the new Leica CM1850. The unit is less than two years old and it already had to be replaced with a new one. I have also worked with the Shandon and Tissue Tek, my favorite is still the Microm. Like with all things what matters is what you feel good about, so I recomend you try them all. If you follow this forum often, you know what I'm talking about. Good luck on your search, Juan -----Original Message----- From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu] Sent: Fri 2/6/2004 11:49 AM To: Histonet Cc: Subject: [Histonet] Cryostats We and our anatomy department is looking for a new cryostat. We would like some input on the different brands. Anatomy has looked at the Brite OTF5000HS, with the 5040 microtome. It is terribly expensive. We have been looking at the Leica and Sakura. However, we haven't looked at the Thermo yet or other brands. Can any of you help us get a handle on what's out there. We will be doing all types of tissues, particularly fat and/or skin. Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Mon Feb 9 11:16:26 2004 From: pruegg <@t> colobio.com (pruegg@colobio.com) Date: Fri Sep 16 15:22:33 2005 Subject: =?US-ASCII?Q?[Histonet]_FW:_[IHCRG]_ck7_and_ck20_?= =?US-ASCII?Q?positive_tumors?= Message-ID: -----Original Message----- From: Patsy Ruegg [mailto:pruegg@msn.com] Sent: Monday, February 09, 2004 9:33 AM To: ihcrg@yahoogroups.com Subject: [IHCRG] ck7 and ck20 positive tumors Dear Friends, Many of those attempting the QIHC exam this go round are in despirate need of tumors positive for cytokeratin 7 and cytokeratin 20. If you can help us obtain any of this tissue for testing we would really appreciate it. I can provide a fed.x number for shippment of tissues to the address listed below. Best regards, Patsy, Chair NSH IHC Resource Group Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 216 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 HP-303-644-4538 hm email pruegg@msn.com wk email pruegg@colobio.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _________________________________________________________________ Get some great ideas here for your sweetheart on Valentine's Day - and beyond. http://special.msn.com/network/celebrateromance.armx ------------------------ Yahoo! Groups Sponsor ---------------------~--> Buy Ink Cartridges or Refill Kits for your HP, Epson, Canon or Lexmark Printer at MyInks.com. Free s/h on orders $50 or more to the US & Canada. http://www.c1tracking.com/l.asp?cid=5511 http://us.click.yahoo.com/mOAaAA/3exGAA/qnsNAA/asSolB/TM ---------------------------------------------------------------------~-> Yahoo! Groups Links <*> To visit your group on the web, go to: http://groups.yahoo.com/group/IHCRG/ <*> To unsubscribe from this group, send an email to: IHCRG-unsubscribe@yahoogroups.com <*> Your use of Yahoo! Groups is subject to: http://docs.yahoo.com/info/terms/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- AdmID:BE6AD80B62FC0A7135901E6F000A7AAC From gcallis <@t> montana.edu Mon Feb 9 11:28:48 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Why not just make TRIS buffer in the lab Message-ID: <3.0.6.32.20040209102848.00bdc0f0@gemini.msu.montana.edu> Instead of making PBS, make up TRIS buffered saline. Make up a 10X solution of TRIS, dilute when you need it 1:10, extremely easy. Here is Chris van der Loos recipe that is excellent, simple - just test pH of 10X - we dilute it 1:10, and the pH is always right on the nose. 10X TRIS (0.5M) Buffered Saline (8.9% to 9%) TRIS base 60.57g/liter Sodium chloride 89 - 90g Conc Hydrochloric acid 30 ml Test pH approx 7.8 We store stock 10X in refrigerator, others may not. Working 0.05M TBS Dilute Stock 10X 1:10 with pure water. We buy our TRIS base from Fisher, with a huge discount. So the buffer is cost effective. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From michael_lafriniere <@t> memorial.org Mon Feb 9 12:26:06 2004 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] CPT coding situation Message-ID: I agree with only one immunohistochemistry charge, however in our lab we would try to place the two sections on one slide to perform the immunohistochemistry, so actually one slide is being stained, thus limiting our cost per slide. Michael LaFriniere PA, HT(ASCP) Pathology Manager Memorial Hospital & Diagnostic Pathology Services -----Original Message----- From: Drew Sally A. [mailto:sa.drew@hosp.wisc.edu] Sent: Monday, February 09, 2004 8:40 AM To: Histonet (E-mail) Subject: RE: [Histonet] CPT coding situation Unfortunately, that would be our approach too, sigh. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Sunday, February 08, 2004 9:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding situation For those of you familiar with CPT coding for surgical pathology, I would like to confirm my interpretation of the following situation: The specimen is a "Left breast, partial mastectomy" and the pathologist found and diagnosed two separate invasive duct carcinomas (tissue blocks 2B and 2K). Ancillary studies (ER, PR, and c-erbB-2) were performed on the two separate tumors. I charged for one set of IHC studies (block 2B), but "no charged" the second set (2K) since both studies were performed on blocks from the same specimen. Is this correct? Thank you. Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbpiontek <@t> hsc.wvu.edu Mon Feb 9 12:55:51 2004 From: dbpiontek <@t> hsc.wvu.edu (Denise Bland-Piontek) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] SLEE Cryostat Message-ID: Does any one know where I can order parts (a 50mm chuck) for the SLEE MEV cryostat? Thanks, Denise Bland-Piontek, HTL(ASCP) West Virginia University From gcallis <@t> montana.edu Mon Feb 9 16:24:16 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Rewrite on TRIS buffer 10X solution In-Reply-To: Message-ID: <3.0.6.32.20040209152416.00bd9060@gemini.msu.montana.edu> Vinnie, I see your point, I didn't indicate volume of water the salts are dissolved in! It is rewritten! TRIS base is Tris(hydorymethl)aminomethane, F.W. aka MW 121.14. 61 g TRIS base and 90 g NaCl is added to approx 800 ml water (these salts go into water very easily! You could use a smaller volume, I have seen 600 ml also), then add 30 mls HCL and bring to final volume (qs) to 1 liter with distilled water. Check pH just before final volume to leave room for either more HCl or sodium hydroxide for pH adjustment. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From naje1972 <@t> yahoo.com Mon Feb 9 16:26:15 2004 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Immunohistochemistry/basic courses Message-ID: <20040209222615.5533.qmail@web41707.mail.yahoo.com> Hello fellow Histonetters I was ask by someone in the lab if there is a community college or university in the Chicagoland or the state of Illinois that teaches immunohistochemistry basic courses. If so would please contact me at 773-342-1559 or e-mail me at cynthiahaynes@hotmail.com Thanks in advance Cynthia Haynes H.T. __________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html From Jenny-Oblander <@t> omrf.ouhsc.edu Fri Feb 6 15:58:21 2004 From: Jenny-Oblander <@t> omrf.ouhsc.edu (Jenny Oblander) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] FW: [COMPMED] Cassettes for an AO Spencer 820 rotary microtome Message-ID: I am forwarding this message for a member of the Comp Med list server. Can anyone help him ? Thanks Jenny -----Original Message----- From: Brian Gordon, DVM Sent: Friday, February 06, 2004 3:08 PM To: Jenny Oblander Subject: FW: [COMPMED] Cassetts for an AO Spencer 820 rotary microtome -----Original Message----- From: Fernando Catalan [mailto:catalanf18@yahoo.com] Sent: Friday, February 06, 2004 2:56 PM To: COMPMED@LISTSERV.AALAS.ORG Subject: [COMPMED] Cassetts for an AO Spencer 820 rotary microtome Hello, Would anyone happen to know where I can find tissue embedding cassetts for an American Optical Spencer 820 rotary microtome? These are very small cassetts with maximum dimensions of about 1.5 X 3 X 0.5 cm. Thank you in advance for any information Fernando Catalan Trinity University TX __________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html From ekaplan <@t> squ.edu.om Mon Feb 9 21:49:06 2004 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] control tissues In-Reply-To: Message-ID: Hi Theresa, Thanks for your reply but I am well familiar with the 'in-house' sytem of control tissues. I am more interested in inocultaing tissues, such as fresh placenta, with 'substances' to create a new control block. Just wondering if anyone has tried it! Regards, Evelyn -----Original Message----- From: Therersa Stegall [mailto:STEGTM@samcstl.org] Sent: Tuesday, February 10, 2004 12:00 AM To: ekaplan@squ.edu.om Subject: Re: [Histonet] control tissues Evelyn: We make many of our own controls, using other cases that are positive for the pathology tested for. Our pathologists keep us informed on + Helicobacter Pylori, and for other things, we use tissue raided from surgicals (such as appendix for Trichrome, non-neoplastic spleen for reticulum, liver for PAS). Good liver for Fe controls is hard to find, some Histonetters in England sent me a batch. I think you could "raid" surgicals, and find nearly any tissue you need for controls. Our computer system allows a natural language search, so it gets easier. Peace, Terre >>> "Evelyn Kaplan" 2/8/2004 12:13:37 AM >>> Good morning, I realise this is a subject which has been previously discussed but I wonder if anyone has had experience of 'manufacturing' their own control blocks? I have a student about to start a project and would apreciate any protocols. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Tue Feb 10 03:05:18 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] RE: Buffer/Alkaline phosphatase stainng sytems Message-ID: Dear Rena, You are right about the fact that phosphate ions inhibit alkaline phosphatase activity. Long time ago we have tested this in practice and since that time we are dedicated TBS users. I have mentioned it in the Immunoenzyme Multiple Staining Methods handbook no.45 (Appendix E.1.2 AP, non-commercial visualization systems). I hope this helps. Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From Rena Fail Date Mon, 09 Feb 2004 11:50:40 -0500 To histonet@lists.utsouthwestern.edu Subject [Histonet] FW: Buffer/Alkaline phosphatase stainng sytems We perform IHC on the DAKO Autostainer, at present we am using a commercial concentrate of TRIS buffer with Tween . We are considering making our buffers. I have been told that phosphate buffers are not compatible with alkaline phosphatase staining systems. Does anyone know of any references to buffer/alkaline phophatase incompatibility? Thank you Rena Fail From wayneholland1959 <@t> msn.com Tue Feb 10 05:56:12 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Basic Immunohistochemical Text for purchase Message-ID: Would anyone have information on a text to buy, on basic immunohistochemical principles. I am looking for a text book on the basics for my staff. We may be introducing automated immuno soon and I would like the staff to have a good introductory text on hand. If anyone has input of what text would be helpful with ordering information, I would be greatful. _________________________________________________________________ Create your own personal Web page with the info you use most, at My MSN. http://click.atdmt.com/AVE/go/onm00200364ave/direct/01/ From syedab <@t> totalise.co.uk Tue Feb 10 05:45:20 2004 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Dako ChemMate Envision kit References: <004a01c3ef28$6b07a7e0$80c401a3@clneuro.ox.ac.uk> Message-ID: <00d801c3efcb$5c90d120$80c401a3@clneuro.ox.ac.uk> Thank you very much for all the helpful suggestions and tips. I've decided that it might be my aliquoted primary that has not been mixed properly and is givign inconsistent results. So I am going to thaw the aliquots and remix and realiquot. Is this a good idea? Anila ----- Original Message ----- From: "Anila Syed" To: Sent: Monday, February 09, 2004 4:18 PM Subject: [Histonet] Dako ChemMate Envision kit > Hi all, > > I have been using the Dako Envision ChemMate kit for three weeks with no > success. I wondered whether I was doing something obviously silly like using > the wrong pH. In the protocol it mentions to use the Dako buffer kit. Could > someone tell me whether it suddenly needs to change pH half way through the > protocol? > I am using PBS at pH 7.4 made up from tablets. > > Thanks in advance > > Anila Syed > > > > --- > Outgoing mail is certified Virus Free. > Checked by AVG anti-virus system (http://www.grisoft.com). > Version: 6.0.580 / Virus Database: 367 - Release Date: 06/02/04 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.580 / Virus Database: 367 - Release Date: 06/02/04 From Jackie.O'Connor <@t> abbott.com Tue Feb 10 06:32:47 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Basic Immunohistochemical Text for purchase Message-ID: Microscopy, Immunohistochemistry and Antigen Retrieval Methods For Light and Electron Microscopy: M.A Hayat 2002 Kluwer Academic/Plenum Publishers, New York I've loaned this book to post-docs who don't have a stitch of IHC experience - it covers all the basics and then some. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics "Wayne Holland" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/10/2004 05:56 AM To: Histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Basic Immunohistochemical Text for purchase Would anyone have information on a text to buy, on basic immunohistochemical principles. I am looking for a text book on the basics for my staff. We may be introducing automated immuno soon and I would like the staff to have a good introductory text on hand. If anyone has input of what text would be helpful with ordering information, I would be greatful. _________________________________________________________________ Create your own personal Web page with the info you use most, at My MSN. http://click.atdmt.com/AVE/go/onm00200364ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Tue Feb 10 06:50:59 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] control tissues Message-ID: At my former lab we tried it with Helicobacter with no success. We did have success with another attempt. I think it was with AFB, but it may have been GMS. My memory is a little hazy this morning as I haven't had my coffee yet. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evelyn Kaplan Sent: Monday, February 09, 2004 9:49 PM To: Therersa Stegall Cc: Histonet Subject: RE: [Histonet] control tissues Hi Theresa, Thanks for your reply but I am well familiar with the 'in-house' sytem of control tissues. I am more interested in inocultaing tissues, such as fresh placenta, with 'substances' to create a new control block. Just wondering if anyone has tried it! Regards, Evelyn -----Original Message----- From: Therersa Stegall [mailto:STEGTM@samcstl.org] Sent: Tuesday, February 10, 2004 12:00 AM To: ekaplan@squ.edu.om Subject: Re: [Histonet] control tissues Evelyn: We make many of our own controls, using other cases that are positive for the pathology tested for. Our pathologists keep us informed on + Helicobacter Pylori, and for other things, we use tissue raided from surgicals (such as appendix for Trichrome, non-neoplastic spleen for reticulum, liver for PAS). Good liver for Fe controls is hard to find, some Histonetters in England sent me a batch. I think you could "raid" surgicals, and find nearly any tissue you need for controls. Our computer system allows a natural language search, so it gets easier. Peace, Terre >>> "Evelyn Kaplan" 2/8/2004 12:13:37 AM >>> Good morning, I realise this is a subject which has been previously discussed but I wonder if anyone has had experience of 'manufacturing' their own control blocks? I have a student about to start a project and would apreciate any protocols. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From aidanhisto <@t> yahoo.co.uk Tue Feb 10 07:46:51 2004 From: aidanhisto <@t> yahoo.co.uk (Aidan Schurr) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] (OT) Back again Message-ID: <004601c3efde$2ae56240$aa671aac@marketinglabpc> Hi Folks, It's been a year or so since I've been on the list, so I though I'd drop a quick note to you all and say a great big "gidday"! For those of you who remember me, I was Section Head of Histology at Hutt Hospital in Wellington New Zealand... Well, in the iterim, I've taken off to the Northern hemisphere, and having been living for the last 12 months in London, England, working as a locum Lab Tech. I've been doing as much travelling as is possible, and generally having a good time... Will be great to chat to you all again. Hope I can still be of some help!! Cheers, Aidan Schurr. From Rcartun <@t> harthosp.org Tue Feb 10 08:01:41 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] control tissues Message-ID: Yes, I frequently inject organisms into fresh lung tissue and then use it as a positive control after formalin fixation and histological processing. Richard Cartun >>> "Evelyn Kaplan" 02/09/04 10:49PM >>> Hi Theresa, Thanks for your reply but I am well familiar with the 'in-house' sytem of control tissues. I am more interested in inocultaing tissues, such as fresh placenta, with 'substances' to create a new control block. Just wondering if anyone has tried it! Regards, Evelyn -----Original Message----- From: Therersa Stegall [mailto:STEGTM@samcstl.org] Sent: Tuesday, February 10, 2004 12:00 AM To: ekaplan@squ.edu.om Subject: Re: [Histonet] control tissues Evelyn: We make many of our own controls, using other cases that are positive for the pathology tested for. Our pathologists keep us informed on + Helicobacter Pylori, and for other things, we use tissue raided from surgicals (such as appendix for Trichrome, non-neoplastic spleen for reticulum, liver for PAS). Good liver for Fe controls is hard to find, some Histonetters in England sent me a batch. I think you could "raid" surgicals, and find nearly any tissue you need for controls. Our computer system allows a natural language search, so it gets easier. Peace, Terre >>> "Evelyn Kaplan" 2/8/2004 12:13:37 AM >>> Good morning, I realise this is a subject which has been previously discussed but I wonder if anyone has had experience of 'manufacturing' their own control blocks? I have a student about to start a project and would apreciate any protocols. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GAshton <@t> PICR.man.ac.uk Tue Feb 10 08:40:24 2004 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] processor Message-ID: Dear all, I am about to buy a new processor. Does anybody have any thoughts / comments about the various ones on the market in the UK, particularly the Shandon models, Leica and Bayer. Many thanks in advance. Garry Paterson Institute Manchester UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From GAshton <@t> PICR.man.ac.uk Tue Feb 10 08:39:22 2004 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] processor Message-ID: Dear all, I am about to buy a new processor. Does anybody have any thoughts / comments about the various ones on the market in the UK, particularly the Shandon models, Leica and Bayer. Many thanks in advance. Garry Paterson Institute Manchester UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From gcallis <@t> montana.edu Tue Feb 10 09:15:09 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] control tissues In-Reply-To: References: Message-ID: <3.0.6.32.20040210081509.00bf05f8@gemini.msu.montana.edu> The original publication on inoculation of placenta is in Histologic, many years ago but available on Sakura Finetek website - a good read. Contact Peggy Wenk, she does this all the time. Also, go to March 2003 issue of J of Histotechnology, a David Young has another tidy method for getting control blocks for Gram staining, Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) At 07:49 AM 2/10/2004 +0400, you wrote: >Hi Theresa, > >Thanks for your reply but I am well familiar with the 'in-house' sytem of >control tissues. I am more interested in inocultaing tissues, such as fresh >placenta, with 'substances' to create a new control block. Just wondering if >anyone has tried it! > >Regards, >Evelyn > >-----Original Message----- >From: Therersa Stegall [mailto:STEGTM@samcstl.org] >Sent: Tuesday, February 10, 2004 12:00 AM >To: ekaplan@squ.edu.om >Subject: Re: [Histonet] control tissues > > >Evelyn: We make many of our own controls, using other cases that are >positive for the pathology tested for. Our pathologists keep us >informed on + Helicobacter Pylori, and for other things, we use tissue >raided from surgicals (such as appendix for Trichrome, non-neoplastic >spleen for reticulum, liver for PAS). Good liver for Fe controls is >hard to find, some Histonetters in England sent me a batch. I think you >could "raid" surgicals, and find nearly any tissue you need for >controls. Our computer system allows a natural language search, so it >gets easier. Peace, Terre > >>>> "Evelyn Kaplan" 2/8/2004 12:13:37 AM >>> > > >Good morning, > >I realise this is a subject which has been previously discussed but I >wonder >if anyone has had experience of 'manufacturing' their own control >blocks? I >have a student about to start a project and would apreciate any >protocols. > >Regards, >Evelyn Kaplan, >Dept of Pathology, >College of Medicine and Health Sciences, >Sultan Qaboos University, >Oman > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From mnunes <@t> ipolisboa.min-saude.pt Tue Feb 10 09:14:58 2004 From: mnunes <@t> ipolisboa.min-saude.pt (Moura Nunes) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Unsubscribe, please Message-ID: <680BB7AA19C9844F9B2F98933335E36331D28C@mail.ipolisboa.min-saude.pt> Unsubscribe J.F. Moura Nunes Lab. Electron Microscopy IPOFG-CROL Lisbon Portugal From DDittus787 <@t> aol.com Tue Feb 10 09:17:26 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] control tissues Message-ID: <25DEC3B5.12515BFB.0A1F969F@aol.com> we have made gram +/_ controls by taking a piece of placenta ,inoculating with a qc culture from our micro dept, with staph and proteus ,incubating to get the buggers to overgrow and then processing. it has worked pretty well for us. dana From gcallis <@t> montana.edu Tue Feb 10 09:21:44 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Basic Immunohistochemical Text for purchase In-Reply-To: Message-ID: <3.0.6.32.20040210082144.00bf05f8@gemini.msu.montana.edu> DAKO has a free IHC manual, titled Immunochemical Staining Methods 3rd Edition, by Boenisch. You can go to DAKOCYTOMATION website and download the pdf file. Polak and van Noorden's new edition is not out yet (over a year late and now will come from a publisher in England, Taylor and Francis. The old but superb first edition is out of print, sadly. Try the free one from DAKO and your DAKO rep might send copies to each of your techs Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Janet.Bonner <@t> FLHOSP.ORG Tue Feb 10 10:15:35 2004 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] GMS Stain Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F5D@fh2k093.fhmis.net> To remove excess silver ,we dip the slides in 4% Chromic acid and rinse in 1% sodium bisulfite, then check under the scope. If too light, start at the silver step again. Remember that generally a solution is first in the process because it affects the remaining steps, either to enhance or remove! Janet -----Original Message----- From: Joyce Cline [mailto:jcline@wchsys.org] Sent: Thursday, February 05, 2004 3:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] GMS Stain I use a coplin jar of distilled water and I add two -three drops of clorox and then dip the slides until the excess silver comes off. Rinse well in water. ----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PM Subject: [Histonet] GMS Stain > I know there is something to use when the slides get left in the silver > solution to long but have forgotten. Can someone refresh my memory? > Thanks > > > > > ********* Email Confidentiality Statement ********* > Visit http://www.saintfrancis.com/emailconf.asp > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From rfail <@t> toolkitmail.com Tue Feb 10 10:12:53 2004 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] preservatives for PBS/TRIS Message-ID: <40290305.90.709e.793846924@toolkitmail.com> Many thanks for the advice re. buffers. Now one more question. Do you have problems with bacterial growth in the non-commercialy prepared concentrated solutions? Do you add anything to retard the growth of bacteria? Rena Fail From SUSANLMCCOY <@t> aol.com Tue Feb 10 10:33:33 2004 From: SUSANLMCCOY <@t> aol.com (SUSANLMCCOY@aol.com) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Ann Preece Message-ID: Hi, Putting together a poster for the 2004 NSH S/C of all the people who's names are attached to awards or scholarships. We have a new award this year from Polysciences in Ann Preece's name. We are looking for a picture of Ann Preece, if you have one you could share or could direct us to someone you think might have one, please let me know. Sincerely, Susan Susan L. McCoy NSH Awards Chairman From dellav <@t> musc.edu Tue Feb 10 11:03:21 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] (OT) Back again Message-ID: Welcome back Aidan. It 's a pleasure to have you back in our ranks even though I am nore than a bit envious of your travel exploits. you'll have to share with us your experiences as a locum tech. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Aidan Schurr" 02/10/04 08:46AM >>> Hi Folks, It's been a year or so since I've been on the list, so I though I'd drop a quick note to you all and say a great big "gidday"! For those of you who remember me, I was Section Head of Histology at Hutt Hospital in Wellington New Zealand... Well, in the iterim, I've taken off to the Northern hemisphere, and having been living for the last 12 months in London, England, working as a locum Lab Tech. I've been doing as much travelling as is possible, and generally having a good time... Will be great to chat to you all again. Hope I can still be of some help!! Cheers, Aidan Schurr. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aidanhisto <@t> yahoo.co.uk Tue Feb 10 11:15:48 2004 From: aidanhisto <@t> yahoo.co.uk (Aidan Schurr) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] (OT) Back again References: Message-ID: <00a401c3eff9$86fdc8e0$aa671aac@marketinglabpc> Hi Vinnie and all, If you are interested in what I've been up to in the last year, check out www.aidanandrach.cjb.net That's our homepage, with lots of photos and stories of where we've been and what we've done. The website imposes a daily limit on traffic, so if you get an error, try again tomorrow ;-) As for being a locum, it was a big, big change from being a manager! I have to say, it was an extremely enlightening experience - the difference between labs in New Zealand and those in the UK is quite profound. The basic methodologies are no different (hands up those who use Bancroft and Stevens?), but the way the systems are implemented is quite distinct. That being said, a month in and it was like I'd been there forever (mostly in a good way, that is!). I was lucky enough to be placed in a long term locum position, with a great bunch of people at UCLH in London, so it made the 12 months go extremely quickly... Regards to all, Aidan ----- Original Message ----- From: "Vinnie Della Speranza" To: ; Sent: Tuesday, February 10, 2004 5:03 PM Subject: Re: [Histonet] (OT) Back again Welcome back Aidan. It 's a pleasure to have you back in our ranks even though I am nore than a bit envious of your travel exploits. you'll have to share with us your experiences as a locum tech. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Aidan Schurr" 02/10/04 08:46AM >>> Hi Folks, It's been a year or so since I've been on the list, so I though I'd drop a quick note to you all and say a great big "gidday"! For those of you who remember me, I was Section Head of Histology at Hutt Hospital in Wellington New Zealand... Well, in the iterim, I've taken off to the Northern hemisphere, and having been living for the last 12 months in London, England, working as a locum Lab Tech. I've been doing as much travelling as is possible, and generally having a good time... Will be great to chat to you all again. Hope I can still be of some help!! Cheers, Aidan Schurr. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Feb 10 11:47:58 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] (no subject) Message-ID: <000801c3effe$2ef44d40$1d2a14ac@wchsys.org> From nick.kirk3 <@t> btopenworld.com Tue Feb 10 12:01:57 2004 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] processor In-Reply-To: Message-ID: Gary I think it's fair to say that most of the models available in the UK do pretty much the same thing, it depends on whether you want a basic processor or one with all the bells and whistles. I currently use the Shandon Pathcentre (I have 2) which is a good workhorse machine. The new Shandon Excelsior looks like a good machine and has several very good Health and Safety features on it. I also have experience of the Leica TP1050 which was a good machine and although I have no experience of their new one with the touch screen, from what I've heard it's a good machine. The Bayer VIP seems to go on forever and is a good workhorse of a machine although I've always been a little concerned about the wax tank on it. Loads have been sold though so it can't be that bad. At the end of the day you "pays your money and you takes your choice" as the saying goes. They all have their pros and cons. My best advice is to have each of them in on demo for a couple of weeks and try them out and see what suits your way of working and more importantly, your price range. Don't ever accept the first quote, always try to barter. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Garry Ashton Sent: 10 February 2004 14:39 To: histonet Subject: [Histonet] processor Dear all, I am about to buy a new processor. Does anybody have any thoughts / comments about the various ones on the market in the UK, particularly the Shandon models, Leica and Bayer. Many thanks in advance. Garry Paterson Institute Manchester UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Feb 10 12:57:41 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] DAKO Handbook Message-ID: <3.0.6.32.20040210115741.00be7d88@gemini.msu.montana.edu> For those of you trying to get this info, use this website: http://www.ihcworld.com/_books/Dako_Handbook.pdf Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From JWEEMS <@t> sjha.org Tue Feb 10 13:57:33 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Liver Pathology Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD630A@exch2.sjha.org> Who would you guys recommend as an expert pathologist in liver disease? (Who would accept consultations.) Thanks, Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From srishan <@t> mail.holyname.org Tue Feb 10 14:11:23 2004 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] (no subject) In-Reply-To: <000801c3effe$2ef44d40$1d2a14ac@wchsys.org> Message-ID: We are currently using a tape coveslipper from sakura. Although this is not a daily occurrence it might affect 10-15% of the slides on certain days. We have tried to follow all the remedies given by sakura and still having the problem. Has anyone else confronted this issue? If so, how was the issue solved? Nirmala Srishan Srishan@mail.holyname.org "Joyce Cline" To Sent by: "Histonet" histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] (no subject) 02/10/2004 12:47 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ljb <@t> medicine.wisc.edu Tue Feb 10 14:33:57 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Liver Pathology Message-ID: Dear Joyce, I would contact Dr. Reza Hafez here at the University of Wisconsin Hospitals and Clinics. He's an incredible physician and the man who strictly critiqued my slides for the practical portion of the HT exam many years ago. If you'd like someone to call to arrange sending materials for consultation I'd call the ladies in specimen receiving at 608-263-8443. Good luck! Cindy LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> "Weems, Joyce" 02/10/04 01:57PM >>> Who would you guys recommend as an expert pathologist in liver disease? (Who would accept consultations.) Thanks, Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Tue Feb 10 15:20:26 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] DAKO Handbook In-Reply-To: <687064.1076423187@jbepc.thurmond-gazes.musc.edu> References: <3.0.6.32.20040210115741.00be7d88@gemini.msu.montana.edu> <3.0.6.32.20040210115741.00be7d88@gemini.msu.montana.edu> Message-ID: <3.0.6.32.20040210142026.00bea970@gemini.msu.montana.edu> I was using Explorer, went to this website http://www.ihcworld.com/_books/Dako_Handbook.pdf AND www.dakocytomation.com (typed word Handbook in search area). I was able to save a copy to my computer via Acrobat Reader 5.0, and then print off the first page without any problems from the ichworld I have no idea what your computer problems are, although I have a Dell PC. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From nena.dimaano <@t> stryker.com Tue Feb 10 15:39:31 2004 From: nena.dimaano <@t> stryker.com (Dimaano, Nena) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Contact Microradiograph film Message-ID: <20BE9059B6FC2D4B9E8FF2A9E2C4EEBD13F260@HOS2KEXCHCL.howost.strykercorp.com> Can anyone recommend a good microradiograph film? thanks a lot, Nena Nena Dimaano,MT/HT(ASCP) Advanced Technology stryker Orthopaedics Mahwah, NJ 07430 tel: 201-831-5338 fax: 201-831-6224 email: Nena.Dimaano@stryker.com From gcallis <@t> montana.edu Tue Feb 10 15:56:36 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Contact Microradiograph film In-Reply-To: <20BE9059B6FC2D4B9E8FF2A9E2C4EEBD13F260@HOS2KEXCHCL.howost. strykercorp.com> Message-ID: <3.0.6.32.20040210145636.00be2888@gemini.msu.montana.edu> Linda Jenkins at jlinda@ces.clemson.edu. Using the technic inside a FAXITRON is interesting and a bit dicey. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From spoulos <@t> saa.ars.usda.gov Tue Feb 10 15:57:44 2004 From: spoulos <@t> saa.ars.usda.gov (Sylvia Poulos) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] polyvinyl sponges in processors Message-ID: Hi all! I have some tissue that has polyvinyl sponge embedded in it. I need to process it but was wondering if anyone knew if it will interfere? I don't mind if it stays there and I cut sections with it in the tissue or if it dissolves in the xylene...just wondering if I should do anything special. If polyvinyl dissolves in xylene will the tissue collapse on itself, and if so, is there anything I can do to prevent this? As always, thanks for the help! Enjoy the day! Sylvia Sylvia P. Poulos USDA-ARS-Animal Physiology Research Unit Athens, GA 30605 706-583-8279 706-542-0399 (fax) From Linke_Noelle <@t> Allergan.com Tue Feb 10 16:08:04 2004 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] DAKO Handbook Message-ID: Ask them to mail you a hard copy. It's MUCH nicer than a printout!!! Noelle Linke, BS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Tuesday, February 10, 2004 1:20 PM To: Joyce Edmonds; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DAKO Handbook I was using Explorer, went to this website http://www.ihcworld.com/_books/Dako_Handbook.pdf AND www.dakocytomation.com (typed word Handbook in search area). I was able to save a copy to my computer via Acrobat Reader 5.0, and then print off the first page without any problems from the ichworld I have no idea what your computer problems are, although I have a Dell PC. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joseph-galbraith <@t> uiowa.edu Tue Feb 10 16:32:20 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Liver Pathology Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2403005B14@uihc-mail1.uihc.uiowa.edu> Joyce: Dr. Frank Mitros at the University of Iowa also has an excellent reputation as a liver pathologist. He also takes consultations. His phone number is 319-356-1760. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Tuesday, February 10, 2004 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver Pathology Who would you guys recommend as an expert pathologist in liver disease? (Who would accept consultations.) Thanks, Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From vbaker60 <@t> yahoo.com Tue Feb 10 18:21:26 2004 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] polyvinyl sponges in processors In-Reply-To: Message-ID: <20040211002126.118.qmail@web12107.mail.yahoo.com> Sylvia, Back in the mid 90's we used these sponges (polyvinyl acetate)as implants in rats for wound healing research. They do not dissolve while processing. Two things though, once we softened the sponges by letting them soak in a very dilute solution of baby shampoo, the results were disastrous. They just disintegrated up on the water bath. The other thing is that if these sponges haven't been softened they can cause havoc on your blade. I used to soak them in a 1% solution of cold ammonium hydroxide for a few minutes before cutting. Also as an extra precaution I let them air dry overnight. Do not use any Histofreeze or other refrigerant. Ice chips can be used in between if you need a little extra moisture. Good luck. Vikki Baker Institute for Cancer Prevention Valhalla, New York 10595 --- Sylvia Poulos wrote: > Hi all! > I have some tissue that has polyvinyl sponge > embedded in it. I need to > process it but was wondering if anyone knew if it > will interfere? I > don't mind if it stays there and I cut sections with > it in the tissue or > if it dissolves in the xylene...just wondering if I > should do anything > special. If polyvinyl dissolves in xylene will the > tissue collapse on > itself, and if so, is there anything I can do to > prevent this? As > always, thanks for the help! > Enjoy the day! Sylvia > > Sylvia P. Poulos > USDA-ARS-Animal Physiology Research Unit > Athens, GA 30605 > 706-583-8279 > 706-542-0399 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Yahoo! Finance: Get your refund fast by filing online. http://taxes.yahoo.com/filing.html From tamamakj <@t> upmc.edu Tue Feb 10 19:10:38 2004 From: tamamakj <@t> upmc.edu (Tamama, Kenichi) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Anti-goat ECL antibody Message-ID: <9122C182D4268F45BCB9E64A10B7331FE8DBBF@1upmc-msx7.isdip.upmc.edu> Dear Histonet members, I would like to know good anti-goat ECL antibody for western blot. We use it from Promega, but the background is very high. Thanks, Kenichi Tamama, M.D., Ph.D. Resident of Laboratory Medicine (Clinical Pathology) University of Pittsburgh From srishan <@t> mail.holyname.org Wed Feb 11 05:34:56 2004 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] (no subject) In-Reply-To: <5D03ED7B9391D4119D9B0008C76B7B2403005B14@uihc-mail1.uihc.uiowa.edu> Message-ID: Hello, We are currently using a tape coverslipper from Sakura. Although this is not a daily occurrence it might affect 10-15% of our slides on certain days. We have tried to follow all the remedy given by Sakura and still having problems. Has anyone else confronted this issue? If so, how was the issue resolved? Nirmala Srishan Srishan@mail.holyname.org From lagehan <@t> hotmail.com Wed Feb 11 08:24:05 2004 From: lagehan <@t> hotmail.com (LORALEE GEHAN) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] over fixing problem Message-ID: I have some samples that were fixed in a rather strong concentration of gluteraldehyde. I know that they are extrememly overfixed. I have cut them with no problem. I am wondering if there is something that I can do to the sections to help with the overfixation. Any advice would be appreciated. Loralee Gehan Orthopaedics Research Lab University of Rochester _________________________________________________________________ Optimize your Internet experience to the max with the new MSN Premium Internet Software. http://click.atdmt.com/AVE/go/onm00200359ave/direct/01/ From pruegg <@t> colobio.com Wed Feb 11 09:29:58 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Colorado Meeting at the Stanley Hotel in Estes Park Message-ID: Don't miss the CSH meeting this year. We are holding a combined meeting with the Mountain States Cytotechnology Group. The Stanely Hotel in Estes Park is a historic land mark in Colorado. Patsy 2004 CSH Meeting Additonal information and registration form are available on the CSH website at http://www.coloradohisto.org Dates: ------ April 23 & 24, 2004 Location: ----------------- The Stanley Hotel (The Shinning was filmed here) 333 Wonderview Ave. Estes Park, CO 80517 Ph: (970) 586-3371 (800) 976-1377 Fx: (970) 586-4964 www.stanleyhotel.com Registration: ------------- Friday: 12:30 - 1:00 Saturday: 7:30 - 8:00 Exhibitors: ----------- Friday: 2:00 - 6:00 Saturday: 9:30 - 5:00 Schedule: --------- Friday, April 23rd ------------------ 12:30 - 1:00 Registration 1:00 - 2:30 Class 1-A or 1-B 2:30 - 3:00 Break in the exhibitor area 3:00 - 4:30 Class 2-A or 2-B 4:30 - 6:00 Social Saturday, April 24th -------------------- 7:30 - 8:00 Registration 8:00 - 9:30 Class 3-A or 3-B 9:30 - 10:00 Break in the exhibitor area 10:00 - 11:30 Class 4-A or 4-C 11:30 - 1:00 Lunch in Exhibitor Area/CSH business meeting 1:00 - 2:30 Class 5-A or 5-B 2:30 - 3:00 Break in the exhibitor area 3:00 - 4:30 Class 6-A or 6-B 4:30 - 5:00 Vendo drawing in the exhibitor area Deadlines: ---------- Hotel Reservations - March 10, 2004 CSH Pre-registration - April 2, 2004 ================================ Friday afternoon, April 23, 2004 ================================ 1-A. HPV Dr. D. Joe Chaffin, M.D. Clinical and Anatomic Pathologist, NCMC This lecture will review the basis of HPV, current techniques utilized for testing and why testing is important. 1-B. Intro to Frozen Sections Ms. Stacey Langenberg, HT(ASCP) QIHC Histologist NCMC This presentation will focus on frozen sections at an introductory level. Topics will include reasons why frozen sections are performed, different procedures, various staining methods, and how far we have come since the beginning of frozen sections. 2-A. Tissue Microarray Dr. Meenakshi Singh, M.D. Associate Director of Surgical Pathology, UCHSC. Tissue microarray (TMA) technology, instrumentation, practical difficulties, potential uses and analysis of TMA on immunohistochemistry sections will be discussed. 2-B. Intro to IHC Ms. Donna Lawson, HT(ASCP) QIHC Technical Trainer, Ventana Medical Systems This introductory level workshop will focus on various types of clones and the basis for which they are chosen. ================================ Saturday Morning, April 24, 2004 ================================ 3-A. Flow Cytometry Dr. Richard Halbert, M.D. Clinical and Anatomic Pathologist NCMC The various uses of Flow Cytometry and the theory behind it will be discussed. 3-B. Ergonomics/Microtomes Ms. Jan Minshew HT(ASCP) HTL Marketing Manager, Leica Microsystems Inc. Correct body mechanics, improved workplace ergonomics as well as proper and safe uses of microtomes will be discussed. 4-A. Melanoma Dr. Richard Halbert, M.D. Clinical and Anatomic Pathologist NCMC This presentation will focus on various markers for melanoma and why they are chosen. 4-B. QIHC Exam Prep Ms. Patsy Ruegg, HT(ASCP) QIHC IHCtech, FitzSimmons Bioscience Park This presentation will familiarize attendees with assignments for the Q-IHC practical exam. Pictures of slides stained for IHC and ISH will be shown. Resources for obtaining Q-IHC exam tissues and reagents that may not be available at many work places will be presented. References for IHC and ASCP application forms will be distributed. ================================== Saturday Afternoon, April 24, 2004 ================================== 5-A. What Stains What Tumor Dr. Alvin W. Martin M.D & Sheron Lear, HT (ASCP) HTL QIHC, University of Louisville, Louisville, KY The presenters will attempt to clarify the use of IHC in diagnostic surgical pathology by demonstrating how antibodies are chosen and how their use impacts diagnosis. A broad range of antibodies will be discussed. Troubleshooting and interpretation difficulties will be addressed. 5-B. CSI/Vegas Ms. Lindsay Sinn, AAPA (Fellow) Manager Surgical Pathology, Laboratory Medicine Consultants, Las Vegas, NV Interesting forensic cases will be discussed as well as how the process works from the standpoint of a death investigator. One case is entitled ?The Silver Bullet?. (Warning, pictures of a graphic nature will be shown.) 6-A. EGFR Dr. Alvin W. Martin M.D & Sheron Lear, HT (ASCP) HTL QIHC, University of Louisville, Louisville, KY Uses of new therapies that are directed at specific mediators of disease, specifically Epidermal Growth Factor Receptor (EGFR) will be discussed. This includes the molecular biology of EGFR , how EGFR functions in tumors, and detection via immunohistochemistry. 6-B. How Dyes Stain Tissue Dr. Dick Dapson, PhD B.S., President, Co-owner, and Director of Research and Marketing Anatech Ltd. Battle Creek, MI. This presentation is designed to bring the topic of dye-tissue interactions to a new level of understanding. The influence of the stain environment (solvent, pH, additives) on dye behavior will be explained as well as the relationship between a dye?s structure and its solubility. Participants will learn how molecular modeling software reveals fascinating information on the ?anatomy and physiology? of dyes, their structure, shape and behavior. From pruegg <@t> colobio.com Wed Feb 11 09:36:07 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy From MAUGER <@t> email.chop.edu Wed Feb 11 10:00:17 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: Patsy, >From my experience it is possible. I would soak the slide in fresh xylene,changing often, until it will lift off without lifting the tissue. The H&E stain can be somewhat lightened with acid alcohol, and 95% alc. The destaining is not really necessary. Depending on the antigen,hopefully it is preserved, the IP staining should work as usual,with a little dirty H&E background. Good luck. Sometimes it takes days for coverslips to come off. Jo >>> "Patsy Ruegg" 02/11/04 10:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Wed Feb 11 13:01:49 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] over fixing problem In-Reply-To: References: Message-ID: <402A7C1D.90101@umdnj.edu> Hi Loralee: Exactly what is the problem with your overfixed tissues? You said that they cut well so ....... Glut fixed tissue does react "abnormally" with some stains, when compared to formalin fixed material. Is that the problem? There are ways to block the aldehyde groups that remain in glut fixed tissue, treatment with sodium borohydride in phos. buffer comes to mind. I can dig up a recipe if you need one. By the way, I have heard the term "overfixed" for many years. I am not really sure that, other than making tissue harder to section, if there really is such a thing for routine light microscopy. Geoff LORALEE GEHAN wrote: > I have some samples that were fixed in a rather strong concentration > of gluteraldehyde. I know that they are extrememly overfixed. I have > cut them with no problem. I am wondering if there is something that I > can do to the sections to help with the overfixation. Any advice > would be appreciated. > > Loralee Gehan > Orthopaedics Research Lab > University of Rochester > > _________________________________________________________________ > Optimize your Internet experience to the max with the new MSN Premium > Internet Software. > http://click.atdmt.com/AVE/go/onm00200359ave/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From m.vankempen <@t> erasmusmc.nl Wed Feb 11 10:09:58 2004 From: m.vankempen <@t> erasmusmc.nl (m. van mkempen) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Flk1,Tal1,Runx and Lmo2 antibodies Message-ID: <402A53D6.2A90143B@erasmusmc.nl> Hello, I am looking for companies that have antibodies against Flk1,Tal1,Runx and Lmo2. Does anybody know were I can find them? Thanks for all your help. Kind Regards! Marta -- ---------------------------- *- mkempen -* MAILTO:m.vankempen@erasmusmc.nl ----------------------------------------- From Terry.Marshall <@t> rothgen.nhs.uk Wed Feb 11 10:15:02 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Flk1,Tal1,Runx and Lmo2 antibodies Message-ID: Requests like this, for things I've never heard of, help keep me humble:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: m. van mkempen [mailto:m.vankempen@erasmusmc.nl] Sent: 11 February 2004 16:10 To: Histonetlist Subject: [Histonet] Flk1,Tal1,Runx and Lmo2 antibodies Hello, I am looking for companies that have antibodies against Flk1,Tal1,Runx and Lmo2. Does anybody know were I can find them? Thanks for all your help. Kind Regards! Marta -- ---------------------------- *- mkempen -* MAILTO:m.vankempen@erasmusmc.nl ----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gsennello <@t> osip.com Wed Feb 11 10:24:13 2004 From: gsennello <@t> osip.com (Sennello, Gina) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Flk1,Tal1,Runx and Lmo2 antibodies Message-ID: R&D and LabVsion sell the Flk-1 Gina Sennello Associate Scientist/ Histotechnologist OSIP 2860 Wilderness Place Boulder, CO 80301 phone 303-546-7739 fax 303-444-0672 -----Original Message----- From: m. van mkempen [mailto:m.vankempen@erasmusmc.nl] Sent: Wednesday, February 11, 2004 9:10 AM To: Histonetlist Subject: [Histonet] Flk1,Tal1,Runx and Lmo2 antibodies Hello, I am looking for companies that have antibodies against Flk1,Tal1,Runx and Lmo2. Does anybody know were I can find them? Thanks for all your help. Kind Regards! Marta -- ---------------------------- *- mkempen -* MAILTO:m.vankempen@erasmusmc.nl ----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Wed Feb 11 10:35:43 2004 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: Patsy, To make the coverslip come off faster, fill a coplin jar with xylene and put the slides in, cover with a top and then put the coplin jar into a 60 degree C. oven and agitate the xylene occaisionally and the coverslip will come off in much less time than soaking at room temperature. It's a little trick I've learned. Peggy DiCarlo HT(ASCP) -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, February 11, 2004 10:36 To: Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: [Histonet] IHC on H&E section I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From RossS <@t> BaylorHealth.edu Wed Feb 11 10:24:44 2004 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: In my experience destaining is not necessary if you are doing a heat epitope retrieval. The retrieval will remove most if not all of the H/E stain. You may have a slight blush of eosin when finished, but it will be easy to distinguish from DAB staining in my experience. I have recently heard that there are some antibodies that may not be preserved after H/E staining, but I have not experienced this personally. AE1/AE3 should be fine. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Wednesday, February 11, 2004 10:00 AM To: pruegg@colobio.com; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: Re: [Histonet] IHC on H&E section Patsy, >From my experience it is possible. I would soak the slide in fresh >xylene,changing often, until it will lift off without lifting the tissue. The H&E stain can be somewhat lightened with acid alcohol, and 95% alc. The destaining is not really necessary. Depending on the antigen,hopefully it is preserved, the IP staining should work as usual,with a little dirty H&E background. Good luck. Sometimes it takes days for coverslips to come off. Jo >>> "Patsy Ruegg" 02/11/04 10:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From MDiCarlo <@t> KaleidaHealth.Org Wed Feb 11 10:28:58 2004 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: Patsy, To make the coverslip come off faster, fill a coplin jar with xylene and put the slides in, cover with a top and then put the coplin jar into a 60 degree C. oven and agitate the xylene occaisionally and the coverslip will come off in much less time than soaking at room temperature. It's a little trick I've learned. Peggy DiCarlo HT(ASCP) -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, February 11, 2004 10:36 To: Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: [Histonet] IHC on H&E section I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From garygill <@t> dcla.com Wed Feb 11 11:01:33 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: Unless the oven's explosion-proof, heating xylene is a potential flammability/explosion hazard -- notwithstanding that the Coplin jar is covered. Gary Gill -----Original Message----- From: DiCarlo, Margaret [mailto:MDiCarlo@KaleidaHealth.Org] Sent: Wednesday, February 11, 2004 11:29 AM To: 'Patsy Ruegg'; Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: RE: [Histonet] IHC on H&E section Patsy, To make the coverslip come off faster, fill a coplin jar with xylene and put the slides in, cover with a top and then put the coplin jar into a 60 degree C. oven and agitate the xylene occaisionally and the coverslip will come off in much less time than soaking at room temperature. It's a little trick I've learned. Peggy DiCarlo HT(ASCP) -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, February 11, 2004 10:36 To: Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: [Histonet] IHC on H&E section I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ALee <@t> mrl.ubc.ca Wed Feb 11 11:32:53 2004 From: ALee <@t> mrl.ubc.ca (Albert Lee) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Pathology Assistant Message-ID: Just wondering how many labs are using certified / trained Pathology Assistants to sign out cases and perform some of the Pathologist's duties? Are there any centres in Canada that are employing these para-pathologists? Any training centres in Canada for this program (only ones I know are at Duke and Indiana State) Thanks to all Albert Lee, BSc., RT (CSMLS) Cardiovascular Registry, the iCAPTURE Centre St. Paul's Hospital Room 166, Burrard Building 1081 Burrard Street, Vancouver, BC Canada V6Z 1Y6 Phone: 604 - 682 - 2344 extension 63572 Fax: 604 - 806 - 8158 From ian.montgomery <@t> bio.gla.ac.uk Wed Feb 11 11:50:54 2004 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Image archiving. Message-ID: <6.0.1.1.2.20040211174024.02d25010@udcf.gla.ac.uk> I'm not advertising, no shares in the company or promise of freebies, but I thought I'd let you know about a new piece of software I've started using for archiving my microscope images. This is the Catalyzer from http://www.axiope.com/. I've been using it since the start of the year and it really is quite superb. An easy and very friendly piece of software, best of all, it won't break the bank. At the moment I'm using it for LM, EM and confocal image archiving but have plans for the future in other areas. Have a look at the web site and try the free trial download, I'm sure you will be impressed. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From settembr <@t> umdnj.edu Wed Feb 11 12:07:35 2004 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: Patsy, Soak in the same reagent that was used before coverslipping. If a xylene substitute was used, soak in that. We use xylene, so we soak in xylene to remove coverslip. Slides coverslipped yesterday come off within an hour. If coverslipped two years ago, it could take days at room temp. I agree with Ross Stapf regarding destaining. It is not really necessary. I use a citra pretreatment in the microwave when running AE1/AE3 and the color left behind is a mild blush. I run immunos on H&E slides often and it works. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> "Stapf, Ross" 2/11/2004 8:24:44 AM >>> In my experience destaining is not necessary if you are doing a heat epitope retrieval. The retrieval will remove most if not all of the H/E stain. You may have a slight blush of eosin when finished, but it will be easy to distinguish from DAB staining in my experience. I have recently heard that there are some antibodies that may not be preserved after H/E staining, but I have not experienced this personally. AE1/AE3 should be fine. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Wednesday, February 11, 2004 10:00 AM To: pruegg@colobio.com; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: Re: [Histonet] IHC on H&E section Patsy, >From my experience it is possible. I would soak the slide in fresh >xylene,changing often, until it will lift off without lifting the tissue. The H&E stain can be somewhat lightened with acid alcohol, and 95% alc. The destaining is not really necessary. Depending on the antigen,hopefully it is preserved, the IP staining should work as usual,with a little dirty H&E background. Good luck. Sometimes it takes days for coverslips to come off. Jo >>> "Patsy Ruegg" 02/11/04 10:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From scoop <@t> mail.nih.gov Wed Feb 11 12:12:44 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] RE: Image archiving. Message-ID: Hi. Thanks for the advice. We need something like this for archiving too. Is Catalyzer like Filemaker Pro or is it different, and, if it's similar, do you have an opinion on which is better? Thanks, Sharon >X-Sender: iom1f@udcf.gla.ac.uk@udcf.gla.ac.uk >Date: Wed, 11 Feb 2004 17:50:54 +0000 >To: histonet@lists.utsouthwestern.edu >From: Ian Montgomery >X-Scan-Signature: caec9e7ca10ae3625b33f2c31db97675 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: , > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 218f69a45ba25cab82cec6de17a14311 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] Image archiving. >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.5 required=6.5 tests=FREE_TRIAL autolearn=no > version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes > > I'm not advertising, no shares in the company or promise of >freebies, but I thought I'd let you know about a new piece of >software I've started using for archiving my microscope images. This >is the Catalyzer from http://www.axiope.com/. I've been using it >since the start of the year and it really is quite superb. An easy >and very friendly piece of software, best of all, it won't break the >bank. At the moment I'm using it for LM, EM and confocal image >archiving but have plans for the future in other areas. > Have a look at the web site and try the free trial download, >I'm sure you will be impressed. >Ian. > >Dr. Ian Montgomery, >Histotechnology, >Graham Kerr Building, >Institute of Biomedical & Life Sciences, >University of Glasgow, >Glasgow, >G12 8QQ. >Tel: 0141 339 8855 >Office: 4652 >Lab: 6644. >Pager: 07625 702883 >e-mail: ian.montgomery@bio.gla.ac.uk > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From northma <@t> ohsu.edu Wed Feb 11 11:54:36 2004 From: northma <@t> ohsu.edu (Mary North) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: When working with a large tumor registry, I was asked to do IHC on many archived H & E slides. To remove the coverslip, I placed the slide on a hot plate until the adhesive softened and then popped off the coverslip by slipping a razor blade under one corner. No tissue was ever lost. While the slide was still slightly warm, I put it into xylene to remove the remaining adhesive. Then I continued down a typical decerating line to 70% ethanol which removed most of the eosin. To remove the hematoxylin, I used periodic acid solution(I don't remember the concentration or time but I think it was similar to the PAS treatment). The pathologist was found that the IHC's on the archived slides where much more brilliant than on freshly cut sections. Mary North, HT(ASCP), HTL Oregon Health & Science University Portland, OR >>> "Patsy Ruegg" 02/11/04 07:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> med.nyu.edu Wed Feb 11 12:40:14 2004 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] NYSHS Call For Nominations (NYSHS Members Only) Message-ID: TO: All New York State Histotechnology Society Members RE: Award nominations for 2004 The NYSHS Awards committee is asking for nominations from its membership for this years awards cycle. In addition to recognition by the society, recipients will also be awarded a small financial grant to further their knowledge and education in the discipline of histotechnology. Applications are due by April 1st 2004 1.Thermo Electron Student Scholarship Award: Is awarded to a histology student or a histotech who wishes to attend a professional meeting. This award is sponsored by thermo Electron inc. and must be used to defray educational expenses.Please send us: 1.. A letter from you, showing evidence of your commitment to continuing education. 2.. Two letters of recommendation from supervisor, pathologists, or histotechnologist 3.. Name and address of your current employer or school, and your current address 2. Dominic Europa Award: Awarded to a long standing NYSHS member (for use towards an educational meeting) who serves as an inspiration to others in the field. Candidate can be a bench tech or a supervisor (preferably not in the limelight). Please submit a nomination and recommendation letter, detailing the nominees contributions . 3.Biogenex Excellence in Education Award: Awarded to any member in good standing, to be used to fund an educational endeavor. This endeavor could be tuition for a class, educational materials or payment for a meeting that is not funded by an employer. To apply for this award, please send a letter outlining how you would benefit educationally from this award, and how it will help you to better serve the profession. This award is sponsored by Biogenex. We encourage e-mail submission of applications and letters. Please submit applications to: Luis Chiriboga NYSHS Awards Chairperson 318 East 15th Street New York, NY 10003 or vial E-mail Luis.chiriboga@med.nyu.edu From lchung <@t> ppmh.org Wed Feb 11 12:39:00 2004 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] IHC on H&E section Message-ID: How do you keep the tissue from falling off the slide after it went through for antigent retrieval? Does it need antigent retrieval process? I put it through "Declere" in the pressure cooker for about 15 minutes. I use regular slide with STA-ON for my H&E stain. Bruce -----Original Message----- From: Dana Settembre [mailto:settembr@umdnj.edu] Sent: Wednesday, February 11, 2004 1:08 PM To: RossS@BaylorHealth.edu; pruegg@colobio.com; MAUGER@email.chop.edu; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: RE: [Histonet] IHC on H&E section Patsy, Soak in the same reagent that was used before coverslipping. If a xylene substitute was used, soak in that. We use xylene, so we soak in xylene to remove coverslip. Slides coverslipped yesterday come off within an hour. If coverslipped two years ago, it could take days at room temp. I agree with Ross Stapf regarding destaining. It is not really necessary. I use a citra pretreatment in the microwave when running AE1/AE3 and the color left behind is a mild blush. I run immunos on H&E slides often and it works. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> "Stapf, Ross" 2/11/2004 8:24:44 AM >>> In my experience destaining is not necessary if you are doing a heat epitope retrieval. The retrieval will remove most if not all of the H/E stain. You may have a slight blush of eosin when finished, but it will be easy to distinguish from DAB staining in my experience. I have recently heard that there are some antibodies that may not be preserved after H/E staining, but I have not experienced this personally. AE1/AE3 should be fine. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Wednesday, February 11, 2004 10:00 AM To: pruegg@colobio.com; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: Re: [Histonet] IHC on H&E section Patsy, >From my experience it is possible. I would soak the slide in fresh >xylene,changing often, until it will lift off without lifting the tissue. The H&E stain can be somewhat lightened with acid alcohol, and 95% alc. The destaining is not really necessary. Depending on the antigen,hopefully it is preserved, the IP staining should work as usual,with a little dirty H&E background. Good luck. Sometimes it takes days for coverslips to come off. Jo >>> "Patsy Ruegg" 02/11/04 10:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tbarnhart <@t> primecare.org Wed Feb 11 13:01:12 2004 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Sep 16 15:22:33 2005 Subject: [Histonet] Pathology Assistant Message-ID: <1779904B5E82D511914C00D0B793339205BFD7D3@exchangent> Pathologist's assistants can not sign out cases, only a Pathologist(MD) may do that. A pathologist's assistant can only do the gross examination of surgical specimens. We use a in-house trained pathologist's assistant to do gross examinations (dictation) and help with autopsy. The trained pathologist's assistant must meet the CLIA educational requirements for high complexity testing. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND tbarnhart@primecare.org -----Original Message----- From: Albert Lee [mailto:ALee@mrl.ubc.ca] Sent: Wednesday, February 11, 2004 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Assistant Just wondering how many labs are using certified / trained Pathology Assistants to sign out cases and perform some of the Pathologist's duties? Are there any centres in Canada that are employing these para-pathologists? Any training centres in Canada for this program (only ones I know are at Duke and Indiana State) Thanks to all Albert Lee, BSc., RT (CSMLS) Cardiovascular Registry, the iCAPTURE Centre St. Paul's Hospital Room 166, Burrard Building 1081 Burrard Street, Vancouver, BC Canada V6Z 1Y6 Phone: 604 - 682 - 2344 extension 63572 Fax: 604 - 806 - 8158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From powell_sa <@t> Mercer.EDU Wed Feb 11 13:21:14 2004 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Pathology Assistant References: Message-ID: <001401c3f0d4$3763db90$e3f2acd1@powellsa1> Hi Albert, Go to www.pathologistsassistants.org for more information on the available programs. Shirley ----- Original Message ----- From: "Albert Lee" To: Sent: Wednesday, February 11, 2004 12:32 PM Subject: [Histonet] Pathology Assistant > Just wondering how many labs are using certified / trained Pathology > Assistants to sign out cases and perform some of the Pathologist's > duties? Are there any centres in Canada that are employing these > para-pathologists? Any training centres in Canada for this program (only > ones I know are at Duke and Indiana State) > > Thanks to all > > Albert Lee, BSc., RT (CSMLS) > Cardiovascular Registry, the iCAPTURE Centre > St. Paul's Hospital > Room 166, Burrard Building > 1081 Burrard Street, Vancouver, BC > Canada V6Z 1Y6 > Phone: 604 - 682 - 2344 extension 63572 > Fax: 604 - 806 - 8158 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROENN <@t> surgery.wisc.edu Wed Feb 11 14:15:55 2004 From: ROENN <@t> surgery.wisc.edu (Drew Allan Roenneburg) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Flk1,Tal1,Runx and Lmo2 antibodies Message-ID: Marta, Chemicon has FLK1 and TALL1 and LMO-4. I don't know if these are the same as your looking for. Drew From juan.gutierrez <@t> christushealth.org Wed Feb 11 14:19:17 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] IHC on H&E section Message-ID: A variation on this theme is using a cigarrette lighter to "melt" the mountant and then pop-off the coverslip with a razor blade. It takes about a minute per slide. Good luck, Juan p.s. You can usually see an air bubble form under the coverslip when is ready to be popped-off. -----Original Message----- From: Mary North [mailto:northma@ohsu.edu] Sent: Wed 2/11/2004 11:54 AM To: pruegg@colobio.com; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: Re: [Histonet] IHC on H&E section When working with a large tumor registry, I was asked to do IHC on many archived H & E slides. To remove the coverslip, I placed the slide on a hot plate until the adhesive softened and then popped off the coverslip by slipping a razor blade under one corner. No tissue was ever lost. While the slide was still slightly warm, I put it into xylene to remove the remaining adhesive. Then I continued down a typical decerating line to 70% ethanol which removed most of the eosin. To remove the hematoxylin, I used periodic acid solution(I don't remember the concentration or time but I think it was similar to the PAS treatment). The pathologist was found that the IHC's on the archived slides where much more brilliant than on freshly cut sections. Mary North, HT(ASCP), HTL Oregon Health & Science University Portland, OR >>> "Patsy Ruegg" 02/11/04 07:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maweber <@t> scripps.edu Wed Feb 11 14:36:58 2004 From: maweber <@t> scripps.edu (Martin Weber) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Ki 67 antibody Message-ID: <402A926A.9080009@scripps.edu> Hello, I want to use a Ki 67 antibody for the detection of human cells in a mouse tissue background. The tissue I want to look at was prefixed with 4% PFA at 4C overnight. I have fresh frozen sections of these tissue samples. It seems to me that for most Ki67 antibodies to work under these circumstances an antigen retrival step is required. Apparantly this is difficult to achieve for fresh frozen sections because they fall off the slide during the heating process. Does anybody know an Ki 67 antibody (polyclonal or monoclonal) that might still work with formalin prefixed fresh frozen sections ? I also heard a rumor about coating slides so that the sections do not fall off anymore or using a special brand of slides ? Should I look rather for a polyclonal or monoclonal antibody ? Any help would be much appreciated. Thanks a lot. Martin Weber M.D. The Scripps Research Institute Dept. of Molecular and Experimental Medicine 10550 North Torrey Pines Road La Jolla, CA 92037 Phone: 858-452-0544 E-Mail: maweber@scripps.edu From wayneholland1959 <@t> msn.com Wed Feb 11 15:14:32 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Pathology Assistant Message-ID: I know this question has been asked from time to time, please forgive me. But, What is the minimum requirement/s for a histologist/histology tech, educational or otherwise, having the OK via CLIA regs, to be allowed to gross non complex specimens? ALso, having not defined non-complex specimens can we define in general these specimens? Help in the correct direction to find these answers, defined in black and white would be greatful . >From: "Barnhart, Tammy" >To: 'Albert Lee' , histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Pathology Assistant >Date: Wed, 11 Feb 2004 13:01:12 -0600 > >Pathologist's assistants can not sign out cases, only a Pathologist(MD) may >do that. A pathologist's assistant can only do the gross examination of >surgical specimens. > >We use a in-house trained pathologist's assistant to do gross examinations >(dictation) and help with autopsy. The trained pathologist's assistant >must >meet the CLIA educational requirements for high complexity testing. > >Tammy Barnhart, BS, HTL(ASCP) >Anatomic Pathology Supervisor >St. Alexius Medical Center >Bismarck, ND >tbarnhart@primecare.org > >-----Original Message----- >From: Albert Lee [mailto:ALee@mrl.ubc.ca] >Sent: Wednesday, February 11, 2004 11:33 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Pathology Assistant > > >Just wondering how many labs are using certified / trained Pathology >Assistants to sign out cases and perform some of the Pathologist's >duties? Are there any centres in Canada that are employing these >para-pathologists? Any training centres in Canada for this program (only >ones I know are at Duke and Indiana State) > >Thanks to all > >Albert Lee, BSc., RT (CSMLS) >Cardiovascular Registry, the iCAPTURE Centre >St. Paul's Hospital >Room 166, Burrard Building >1081 Burrard Street, Vancouver, BC >Canada V6Z 1Y6 >Phone: 604 - 682 - 2344 extension 63572 >Fax: 604 - 806 - 8158 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Confidentiality Notice:This e-mail message is for sole use of intended >recipient(s) and may contain confidential and privileged information. Any >unauthorized review, use, disclosure, distribution, or copying is >prohibited. If you are not the intended recipient, please contact the >sender >by replying to this e-mail and destroy/delete all copies of this e-mail >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get some great ideas here for your sweetheart on Valentine's Day - and beyond. http://special.msn.com/network/celebrateromance.armx From lizchlipala <@t> premierhistology.com Wed Feb 11 15:39:02 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] aniline blue for trichrome Message-ID: <000001c3f0e7$7abaf410$74d48a80@LIZ> Hello all We are having a problem with our trichrome stain, we are not getting the correct differentiation of muscle. We normally don't have any problems with this stain, since we always use fresh reagents. We had an old bottle of aniline blue from EMS that we have been using, I had ordered a new bottle from Harleco they are both the same CI - 42755. We started having problems with the new aniline blue, but I'm not certain that this is the reason why since what is not staining in the tissues is the smooth muscle of vessels. We have run this stain on rat and mouse livers, both from carbon tetrachloride induced liver toxicity studies, the morphological changes in this model include fibrosis, necrosis and degenerating hepatocytes. The rat livers look fine, except that the smooth muscle of the vessels are not staining red. The mouse livers also have this problem, but in addition to that the degenerating hepatocytes are staining slightly blue. Also, when I checked into ordering additional aniline blue from Sigma they use Methyl Blue, water soluable - certified for use as Aniline Blue, but the CI is different its 42780. Any suggestions would be helpful. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Barry.R.Rittman <@t> uth.tmc.edu Wed Feb 11 16:21:30 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] aniline blue for trichrome Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0635956@UTHEVS3.mail.uthouston.edu> Hi Liz Technically methyl blue can be substituted for aniline blue WS. They are both very acidic stains with sulfonate groups, similar absorption and therefore similar color. The problem I had with methyl blue was that it did not give as intense a color to connective tissue as the aniline blue WS. I believe that the reason that methyl blue is substituted for aniline blue WS is that it is easier to control the manufacturing process. Aniline blue WS is a mixture of phenyl pararosanilines and I am told that producing a consistent batch is difficult. I would recommend looking at the original bottle that you had success with and not only checking the CI but also the dye content and the batch number. If you can get another bottle of the same then I would lay in a stock of this. While suppliers don't like to do so they will often supply a dye of specific batch number if you request this and if they have it in stock. Wish I could suggest another solution. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, February 11, 2004 3:39 PM To: 'HistoNet Server' Subject: [Histonet] aniline blue for trichrome Hello all We are having a problem with our trichrome stain, we are not getting the correct differentiation of muscle. We normally don't have any problems with this stain, since we always use fresh reagents. We had an old bottle of aniline blue from EMS that we have been using, I had ordered a new bottle from Harleco they are both the same CI - 42755. We started having problems with the new aniline blue, but I'm not certain that this is the reason why since what is not staining in the tissues is the smooth muscle of vessels. We have run this stain on rat and mouse livers, both from carbon tetrachloride induced liver toxicity studies, the morphological changes in this model include fibrosis, necrosis and degenerating hepatocytes. The rat livers look fine, except that the smooth muscle of the vessels are not staining red. The mouse livers also have this problem, but in addition to that the degenerating hepatocytes are staining slightly blue. Also, when I checked into ordering additional aniline blue from Sigma they use Methyl Blue, water soluable - certified for use as Aniline Blue, but the CI is different its 42780. Any suggestions would be helpful. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.P.Whitaker <@t> uth.tmc.edu Wed Feb 11 17:35:34 2004 From: Bonnie.P.Whitaker <@t> uth.tmc.edu (Bonnie P Whitaker) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] plastic laminate countertops Message-ID: <6937f6a13e.6a13e6937f@uth.tmc.edu> Hi All, Have any of you recently built or remodeled your lab using plastic laminate countertops? If so, did you use chemical-resistant laminate or regular? Open to all opinions and suggestions! Thanks! Bonnie Whitaker From kwuny <@t> email.cs.nsw.gov.au Wed Feb 11 17:40:56 2004 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] IHC on H&E section Message-ID: <01C3F154.B2E75DC0.kwuny@email.cs.nsw.gov.au> Instead of using xylene or heating, another variation on this theme is putting the old slides in the freezer for few hours and the coverslip will be popped-off easy. Young Kwun Concord Hospital Sydney, Australia -----Original Message----- From: Dana Settembre [SMTP:settembr@umdnj.edu] Sent: Thursday, 12 February 2004 05:08 To: RossS@BaylorHealth.edu; pruegg@colobio.com; MAUGER@email.chop.edu; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: RE: [Histonet] IHC on H&E section Patsy, Soak in the same reagent that was used before coverslipping. If a xylene substitute was used, soak in that. We use xylene, so we soak in xylene to remove coverslip. Slides coverslipped yesterday come off within an hour. If coverslipped two years ago, it could take days at room temp. I agree with Ross Stapf regarding destaining. It is not really necessary. I use a citra pretreatment in the microwave when running AE1/AE3 and the color left behind is a mild blush. I run immunos on H&E slides often and it works. Dana Settembre University Hospital - UMDNJ, Newark, NJ >>> "Stapf, Ross" 2/11/2004 8:24:44 AM >>> In my experience destaining is not necessary if you are doing a heat epitope retrieval. The retrieval will remove most if not all of the H/E stain. You may have a slight blush of eosin when finished, but it will be easy to distinguish from DAB staining in my experience. I have recently heard that there are some antibodies that may not be preserved after H/E staining, but I have not experienced this personally. AE1/AE3 should be fine. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Wednesday, February 11, 2004 10:00 AM To: pruegg@colobio.com; histonet@pathology.swmed.edu Cc: ihcrg@yahoogroups.com Subject: Re: [Histonet] IHC on H&E section Patsy, >From my experience it is possible. I would soak the slide in fresh >xylene,changing often, until it will lift off without lifting the tissue. The H&E stain can be somewhat lightened with acid alcohol, and 95% alc. The destaining is not really necessary. Depending on the antigen,hopefully it is preserved, the IP staining should work as usual,with a little dirty H&E background. Good luck. Sometimes it takes days for coverslips to come off. Jo >>> "Patsy Ruegg" 02/11/04 10:36AM >>> I know this has come up before but don't remember the details. I would like to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained and coverslipped for some time now. Please advise. Is there a preferred method for removing the coverslip and maintaining the section integrety? Destaining the H&E? Doing the IHC stain on this section? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Central Sydney Area Health Service." From laurie.colbert <@t> huntingtonhospital.com Wed Feb 11 17:50:53 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Special Stains and Internal Controls Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0CAD@EXCHANGE1.huntingtonhospital.com> We want to stop running control tissue for retic and trichrome stains when the patient tissue is liver since liver is an internal control for these stains. Are there others out there who do this and have you ever had a problem during an inspection? I am concerned about question ANP.21400 on the CAP checklist that says, "Are controls run routinely on all special stains, with reactivity results documented, and are they verified for acceptability before reporting results?" Laurie Colbert Huntington Hospital Pasadena, CA From michael_lafriniere <@t> memorial.org Wed Feb 11 18:49:45 2004 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Special Stains and Internal Controls Message-ID: Hi Laurie, As an inspector myself, I would site a lab with a phase II deficiency if found that the laboratory failed to run a positive control with at least each run (could be several slides for one type of stain) for special stains. It would be important that you and the pathologist know the special stain worked by following the results of your control, which you are familiar and confident with. In addition to, each special stain run demonstrating consistent results using your current and past control material as a guide. I would like to suggest that you run either one control with each run (could be several slides for trichrome stain etc.)or place the control on the same slide as the test tissue thus eliminating additional expense on the automatic stainers, there are many slides available on the market to have a control on the same slide as the test tissue. In addition we often find that we can make a "sausage" control block to use as several different controls (several pieces of different control tissue in the same block) and make hundreds of pre made control slides for special stains using one block, this type of block not only works as a positive control but acts additionally as a negative control. I hope this has helped you dilemma, feel free to call me if I can be of additional assistance. Michael LaFriniere PA,HT(ASCP) Pathology Manager Memorial Hospital & Diagnostic Pathology Services Chattanooga TN 423-495-6117 -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Wednesday, February 11, 2004 6:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stains and Internal Controls We want to stop running control tissue for retic and trichrome stains when the patient tissue is liver since liver is an internal control for these stains. Are there others out there who do this and have you ever had a problem during an inspection? I am concerned about question ANP.21400 on the CAP checklist that says, "Are controls run routinely on all special stains, with reactivity results documented, and are they verified for acceptability before reporting results?" Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> earthlink.net Wed Feb 11 20:21:32 2004 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] IHC on H&E section In-Reply-To: References: Message-ID: <6.0.0.22.0.20040211211246.02a35260@127.0.0.1> Patsy, Don't bother destaining it. It's extra steps that increase the odds of having the tissue fall off. Once you get the coverslip off running the slide back to water thru alcohol will take out most of the eosin. The pretreatment (whatever it is) will probably take out the rest. Unless you are not using hematoxylin as a counterstain just leave it on. Less you need to worry about afterword. Amos Brooks At 12:51 PM 2/11/04, you wrote: >Message: 17 >Date: Wed, 11 Feb 2004 08:36:07 -0700 >From: "Patsy Ruegg" >Subject: [Histonet] IHC on H&E section >To: "Histonet@Pathology. Swmed. Edu" >Cc: "Ihcrg@Yahoogroups. Com" >Message-ID: >Content-Type: text/plain; charset="iso-8859-1" > >I know this has come up before but don't remember the details. I would like >to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained >and coverslipped for some time now. Please advise. Is there a preferred >method for removing the coverslip and maintaining the section integrety? >Destaining the H&E? Doing the IHC stain on this section? >Patsy From jnocito <@t> satx.rr.com Wed Feb 11 22:57:23 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] plastic laminate countertops References: <6937f6a13e.6a13e6937f@uth.tmc.edu> Message-ID: <004f01c3f124$b42ef9c0$70494542@satx.rr.com> Bonnie, for what it's worth, we had chemical resistant laminate put in, but it wasn't stain resistant. Even though we had absorbent, plastic lines lab mats, our special stain counter is really specially stained. Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas ----- Original Message ----- From: "Bonnie P Whitaker" To: Sent: Wednesday, February 11, 2004 3:35 PM Subject: [Histonet] plastic laminate countertops > Hi All, > Have any of you recently built or remodeled your lab using plastic > laminate countertops? If so, did you use chemical-resistant laminate > or regular? Open to all opinions and suggestions! > Thanks! > Bonnie Whitaker > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From antje.marcantonio <@t> pharma.novartis.com Thu Feb 12 02:45:20 2004 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] von Willebrand factor IHC in rat kidneys Message-ID: Hello, I'm dealing with a strange phenomena. I have a nice working protocol for vWf in rat FFPE lung or heart, including a 15 min pretreatment of trypsin, 1 hour incubation of primary antibody (Dako) 1:500 followed by Envision/HRP. I get constantly strong and clean staining. The same protocol used for kidneys (yes, fixed and processed with the exactly same schedule) shows only weak staining in the vessels and very weak signal in the glomeruli. I tried to use the primary more concentrated (up to 1:100) and to prolong the its incubation to 2 hours with only little improvement. Any ideas ? Should I consider another enzyme for pretreatment ? Longer digestion with trypsin simply increased the background ,not the signal. Is there any explanation why a protocol which works nicely in lung and heart is not applicable for the kidneys? Thanks to everybody, Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland From M.Bromley <@t> dgri.scot.nhs.uk Thu Feb 12 05:35:09 2004 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] substance p Message-ID: <7325D637DFE2D211928800902733A7F303B94A39@DGAMTBDCEMS> Hi All Has anyone got a good detection method for substance P using immunohistochemistry on formalin fixed paraffins. Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > From stevemachinuk <@t> yahoo.co.uk Thu Feb 12 06:15:26 2004 From: stevemachinuk <@t> yahoo.co.uk (=?iso-8859-1?q?Steve=20Machin=20UK?=) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Fli-1 antibody Message-ID: <20040212121526.24719.qmail@web25101.mail.ukl.yahoo.com> Does anyone know of a UK supplier of Fli-1. I understand that Santa Cruz does it but I dont have a UK supplier for them. Best Wishes Steve Machin UK ___________________________________________________________ BT Yahoo! Broadband - Free modem offer, sign up online today and save ?80 http://btyahoo.yahoo.co.uk From mprice26 <@t> juno.com Thu Feb 12 08:06:30 2004 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] CPT codes Message-ID: <20040212.060731.25056.2133777@webmail20.nyc.untd.com> Histonetters, Does anyone know what CPT code I need to use for Tease Fiber Preparation & for the Faxitron? Thank you in advance. Marsha Price ________________________________________________________________ The best thing to hit the Internet in years - Juno SpeedBand! Surf the Web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From mariac <@t> creighton.edu Thu Feb 12 09:02:35 2004 From: mariac <@t> creighton.edu (Maria Christensen) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Anti-goat ECL antibody In-Reply-To: <9122C182D4268F45BCB9E64A10B7331FE8DBBF@1upmc-msx7.isdip.upmc.edu> References: <9122C182D4268F45BCB9E64A10B7331FE8DBBF@1upmc-msx7.isdip.upmc.edu> Message-ID: Kenichi: I don't do western blotting, but the person in our lab who does also has had high background with Promega's anti-goat Ab. He recommends Vector's anti-goat Ab, PI-9500, as having lower background and overall better results in his westerns. Hope this helps. Maria Maria A. Christensen Technical Associate Medical Microbiology & Immunology Creighton University School of Medicine 2500 California Plaza Omaha, NE 68178-0404 phone (402) 280-2678 FAX (402) 280-1875 email mariac@creighton.edu >Dear Histonet members, > >I would like to know good anti-goat ECL antibody for western blot. We use it >from Promega, but the background is very high. > >Thanks, > >Kenichi Tamama, M.D., Ph.D. > >Resident of Laboratory Medicine (Clinical Pathology) > >University of Pittsburgh > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Feb 12 09:30:37 2004 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] CPT coding situation Message-ID: Yes that would be correct. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Sunday, February 08, 2004 9:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT coding situation For those of you familiar with CPT coding for surgical pathology, I would like to confirm my interpretation of the following situation: The specimen is a "Left breast, partial mastectomy" and the pathologist found and diagnosed two separate invasive duct carcinomas (tissue blocks 2B and 2K). Ancillary studies (ER, PR, and c-erbB-2) were performed on the two separate tumors. I charged for one set of IHC studies (block 2B), but "no charged" the second set (2K) since both studies were performed on blocks from the same specimen. Is this correct? Thank you. Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Ronnie_Houston <@t> bshsi.com Thu Feb 12 10:03:00 2004 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] CPT codes Message-ID: <530361BF03351B4CAE5270A05D3037B50367307C@bsrexms01.BSHSIR.COM> 88362 for nerve teasing preparations haven't a clue as to Faxitron Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboartories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 (804) 287 7906 - fax ronnie_houston@bshsi.com -----Original Message----- From: mprice26@juno.com [mailto:mprice26@juno.com] Sent: Thursday, February 12, 2004 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT codes Histonetters, Does anyone know what CPT code I need to use for Tease Fiber Preparation & for the Faxitron? Thank you in advance. Marsha Price ________________________________________________________________ The best thing to hit the Internet in years - Juno SpeedBand! Surf the Web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From mprice26 <@t> juno.com Thu Feb 12 10:40:13 2004 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Another Billing Question (CPT) Message-ID: <20040212.084028.25056.2136645@webmail20.nyc.untd.com> Histonetters, I apologize for bombarding you with so many billing questions. I have been out of billing for a while and focused on technical. My question is (if I remember Correctly): 1. With Medicare shouldn't the testing facility charge for the procedures done? Or are there exceptions? This is on Cytogenetics and Flow Cytometry that we send out. Thank you. Marsha Price ________________________________________________________________ The best thing to hit the Internet in years - Juno SpeedBand! Surf the Web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From ian.montgomery <@t> bio.gla.ac.uk Thu Feb 12 11:01:15 2004 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:34 2005 Subject: Fwd: [Histonet] RE: Image archiving. Message-ID: <6.0.1.1.2.20040212165817.02d3feb0@udcf.gla.ac.uk> Sharon, Don't know anything about Filemaker Pro so cannot answer. Why don't you contact the people at Axiope, they're friendly so will be delighted to help. Best person to speak to is Fred Howell fred@axiope.com he's a computer buff so will answer all your questions. Ian. >Hi. Thanks for the advice. We need something like this for archiving >too. Is Catalyzer like Filemaker Pro or is it different, and, if it's >similar, do you have an opinion on which is better? > >Thanks, >Sharon > >>X-Sender: iom1f@udcf.gla.ac.uk@udcf.gla.ac.uk >>Date: Wed, 11 Feb 2004 17:50:54 +0000 >>To: histonet@lists.utsouthwestern.edu >>From: Ian Montgomery >>X-Scan-Signature: caec9e7ca10ae3625b33f2c31db97675 >>X-BeenThere: histonet@lists.utsouthwestern.edu >>X-Mailman-Version: 2.1.3 >>List-Id: For the exchange of information pertaining to histotechnology and >> related fields >>List-Unsubscribe: >>, >> >> >>List-Archive: >>List-Post: >>List-Help: >>List-Subscribe: , >> >>Sender: histonet-bounces@lists.utsouthwestern.edu >>X-Scan-Signature: 218f69a45ba25cab82cec6de17a14311 >>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >>Subject: [Histonet] Image archiving. >>X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >>X-Spam-Level: >>X-Spam-Status: No, hits=0.5 required=6.5 tests=FREE_TRIAL autolearn=no >> version=2.63 >>X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >>X-SA-Exim-Scanned: Yes >> >> I'm not advertising, no shares in the company or promise of >> freebies, but I thought I'd let you know about a new piece of software >> I've started using for archiving my microscope images. This is the >> Catalyzer from http://www.axiope.com/. I've been using it since the >> start of the year and it really is quite superb. An easy and very >> friendly piece of software, best of all, it won't break the bank. At the >> moment I'm using it for LM, EM and confocal image archiving but have >> plans for the future in other areas. >> Have a look at the web site and try the free trial download, I'm >> sure you will be impressed. >>Ian. >> >>Dr. Ian Montgomery, >>Histotechnology, >>Graham Kerr Building, >>Institute of Biomedical & Life Sciences, >>University of Glasgow, >>Glasgow, >>G12 8QQ. >>Tel: 0141 339 8855 >>Office: 4652 >>Lab: 6644. >>Pager: 07625 702883 >>e-mail: ian.montgomery@bio.gla.ac.uk >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >-- >Sharon Cooperman >NIH, NICHD, CBMB 301.435-7735 >Building 18T, room 101 301.402-0078 fax >Bethesda, MD 20892 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From Jacquie.Mack <@t> CLS.ab.ca Thu Feb 12 11:09:33 2004 From: Jacquie.Mack <@t> CLS.ab.ca (Jacquie.Mack@CLS.ab.ca) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] unsubscribe Message-ID: <30C050525B881C4AAFF41E6D16543E6803488B42@mail3.cls.ab.ca> please unsubscribe This is my third attempt to unsubscribe. Is this the proper channel of unsubscribing? Thanks JM Jacqueline Mack Tech III , Anatomic Pathology Foothills Medical Center Calgary Laboratory Services ( (403)-944-4162 fax: (403)-270-4093 pager: 212-8223 # 0540 * Ja cquie.Mack@CLS.ab.ca Laboratory Medicine Enhancing Your Health Care From ramona.tolliver <@t> yale.edu Thu Feb 12 11:14:55 2004 From: ramona.tolliver <@t> yale.edu (Ramona Tolliver) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Yale University - Opening Message-ID: <5.2.0.9.2.20040212120658.01f32998@email.med.yale.edu> Good afternoon, Yale University has a 2nd Shift Histology Supervisor Position available from 3:00 -11:00 p.m. If you know of anyone who may be interested in a great opportunity with advancement potential, please forward this information on to them. The posting is located at : http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=124471. Salary starts at 65K and is commensurate with experience. Relocation assistance is available. Thank you for your time! Ramona Tolliver Ramona E. Tolliver Human Resource Manager Yale University School of Medicine Department of Pathology 310 Cedar Street New Haven, CT 06520-8023 Telephone: (203) 785-6689 Fax: (203) 785-7303 From lefortb <@t> crhsc.umontreal.ca Thu Feb 12 11:29:56 2004 From: lefortb <@t> crhsc.umontreal.ca (Bertrand Lefort) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] RE: Image archiving. In-Reply-To: Message-ID: Hello, I am using Access, as it is provided by the University, to store my pictures path. The advantage is that you can store the protocol at the same time. Filemaker Pro could do exactely the same work. I have heard about IQBASe from MediaCybernetics http://www.mediacy.com/. This software is a little bit expansive but seems to be very powerfull. Bertrand From rcharles <@t> state.pa.us Thu Feb 12 13:11:04 2004 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] IHC background stain Message-ID: Hello all, I do all the IHC for our diagnostic veterinary laboratory. Latley i have been getting a lot of background stain from certain protocols. I also over heard one of the pathologists state the sections are getting thicker. Could thick section cause background stain on IHC? I checked my histologist microtome and it is set at 5um. Could her microtome need calibrated? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From leopold <@t> mnsi.net Thu Feb 12 13:25:54 2004 From: leopold <@t> mnsi.net (Derek and/or Lynda Leopold) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Critical Incident? Message-ID: <402BD342.4040201@mnsi.net> Hi Histonetters, I am hoping to get some opinion off-list from supervisors (and anyone else who may care to comment) on an event which happened recently in our lab. To be as short as possible, the person who was responsible for changing the solutions on our Tissue Tek 5 biopsy processor somehow (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of 95%), and the second alcohol to 88% (instead of 95%). For context, we do a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 changes of 95%, then 3 changes of 100% and on to xylene and paraffin. Now that you have the situation, I have two questions: 1) What sorts of changes, if any, would one expect in the biopsy tissues (endo, cervix, etc) exposed to this miscalculated regimen ? 2) If, as some of us surmise, this mistake in solution calculation could be linked to biopsy tissues that were too dehydrated and brittle to be of any diagnostic use, should that constitute a critical error/reportable mistake? We have apparently never experienced such terrible tissues in recent memory, and there is some debate as to whether the low-concentration alcohols could have been the cause. I look forward to your very expert opinions! Thanks Lynda Leopold Harrow, Ontario From RITA.ANGEL <@t> UC.EDU Thu Feb 12 14:19:58 2004 From: RITA.ANGEL <@t> UC.EDU (Rita Angel) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] massons trichrome Message-ID: <5.1.0.14.2.20040212151129.00b69470@ucmail3.uc.edu> hello all, I was hoping someone could help us with a trichrome problem. We are suddenly having problems with the muscle in heart tissue staining purple instead of red. Our skin control is staining the same way. There are 2 techs in the lab, and we're both having problems. The problems are with complete batches, not slides within the batch. We each stained 2 batches in a row, one batch stained with the purple muscle, and the 2nd batch with red muscle. We're leaving the slides in the solutions the same amount of time and rinsing the same amount of times, etc. We're using the same solutions from the same bottles, etc. (we make all of our own solutions). We are unable to think of what could be causing the problem. Also, our slides are still really black when they are finished rinsing after the wiegert's step. Could this be causing it? I appreciate any advice. Thanks, Rita Angel, HT University of Cincinnati From JSCHUMA1 <@t> FAIRVIEW.ORG Thu Feb 12 14:44:08 2004 From: JSCHUMA1 <@t> FAIRVIEW.ORG (JENNIFER SCHUMACHER) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Critical Incident? Message-ID: Unfortunately, I do not have an answer but another question to add . . . we have had two incidents in the last year in which we believe we had a contamination problem, and our tissues were mostly destroyed. Some samples had to be repeated, and our pathologists are determined to find someone to blame. Has this ever happened to anyone else? Were you able to pinpoint the problem, and how can you "guarantee" it will never happen again? All suggestions are welcome, offline is fine if you are not comfortable answering online. Thanks. Jennifer >>> Derek and/or Lynda Leopold 02/12/04 01:25PM >>> Hi Histonetters, I am hoping to get some opinion off-list from supervisors (and anyone else who may care to comment) on an event which happened recently in our lab. To be as short as possible, the person who was responsible for changing the solutions on our Tissue Tek 5 biopsy processor somehow (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of 95%), and the second alcohol to 88% (instead of 95%). For context, we do a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 changes of 95%, then 3 changes of 100% and on to xylene and paraffin. Now that you have the situation, I have two questions: 1) What sorts of changes, if any, would one expect in the biopsy tissues (endo, cervix, etc) exposed to this miscalculated regimen ? 2) If, as some of us surmise, this mistake in solution calculation could be linked to biopsy tissues that were too dehydrated and brittle to be of any diagnostic use, should that constitute a critical error/reportable mistake? We have apparently never experienced such terrible tissues in recent memory, and there is some debate as to whether the low-concentration alcohols could have been the cause. I look forward to your very expert opinions! Thanks Lynda Leopold Harrow, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> From SJones <@t> cvm.tamu.edu Thu Feb 12 14:52:47 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] plastic laminate countertops Message-ID: Hi Bonnie, Our lab has regular laminate and they have held up well for 20 years. It is black in color, so no problems with stains! Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Bonnie P Whitaker" 2/11/2004 5:35:34 PM >>> Hi All, Have any of you recently built or remodeled your lab using plastic laminate countertops? If so, did you use chemical-resistant laminate or regular? Open to all opinions and suggestions! Thanks! Bonnie Whitaker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Thu Feb 12 15:00:59 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] unsubscribe Message-ID: I think you have to go to this web site: http://lists.utsouthwestern.edu/mailman/listinfo/histonet and follow the instructions. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> 2/12/2004 11:09:33 AM >>> please unsubscribe This is my third attempt to unsubscribe. Is this the proper channel of unsubscribing? Thanks JM Jacqueline Mack Tech III , Anatomic Pathology Foothills Medical Center Calgary Laboratory Services ( (403)-944-4162 fax: (403)-270-4093 pager: 212-8223 # 0540 * Ja cquie.Mack@CLS.ab.ca Laboratory Medicine Enhancing Your Health Care _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Feb 12 15:36:09 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] ER/PR pictures Message-ID: <006901c3f1b0$3b0aaf80$1d2a14ac@wchsys.org> Does anyone know of any IHC book or photographs that show the differences in ER/PR grading 1+,2+,3+ etc. and how to distinguish one from the other? I have paths that are not use to looking at ER/PR and want to know the differences. From peptolab <@t> hamptons.com Thu Feb 12 15:41:09 2004 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Factor VIII staining in the kidney Message-ID: <006e01c3f1b0$f2403c60$95a5bd18@JEFF> Antje- could it be physiological- maybe there is really less signal (less Weibel Palade bodies?) in the endothelia of the renal vascular bed? Jeff Silverman Southside Hospital Bay Shore NY USA From Saundra_Patrick <@t> brown.edu Thu Feb 12 16:19:10 2004 From: Saundra_Patrick <@t> brown.edu (Saundra Patrick) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] freezing microtome disposable blade holder Message-ID: <5.2.1.1.2.20040212170437.028b6cb0@postoffice.brown.edu> Hi Everyone, This is my first experience using histonet. It was suggested to me that I might find through histonet, a blade holder for our very old (but still working well)American Optical 860 freezing microtome. We are presently using a permanant steel blade that needs sharpening periodically. We would like to try using disposable blades but have no disposable blade holder for this microtome. Here are my two questions. 1)Does anyone know where we might purchase a blade holder that will fit our microtome? It is the grandfather of microtomes. 2)Would we be compromising quality of the sectioned slice by using disposable blades instead of using our permanent blade? We use the microtome for slicing frozen paraformaldehyde-fixed rodent brain tissue at 30 to 80 um thickness. Thanks, Saundy From dellav <@t> musc.edu Thu Feb 12 16:42:01 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] massons trichrome Message-ID: Rita, jt is difficult to give you anything better than a guess without seeing what the stain looks like. However, it is entirely possible that what may appear to be aniline blue in the muscle cells is actually weigert's hematoxylin. which can remain if the water washes following weigerts hematoxylin staining are insufficient to remove excess stain from non-nuclear tissues. I like to rinse weigert's stained sections in 50- 70% ethanol (8-10 dips in a coplin jar) following the wiegert's stain which is then followed by the water rinse in order to achieve a more thorough removal of the excess stain. You will note that the working weigerts solution contains approx 50% alcohol and will more readily come out in a dilute alcohol than water alone. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> Rita Angel 02/12/04 03:19PM >>> hello all, I was hoping someone could help us with a trichrome problem. We are suddenly having problems with the muscle in heart tissue staining purple instead of red. Our skin control is staining the same way. There are 2 techs in the lab, and we're both having problems. The problems are with complete batches, not slides within the batch. We each stained 2 batches in a row, one batch stained with the purple muscle, and the 2nd batch with red muscle. We're leaving the slides in the solutions the same amount of time and rinsing the same amount of times, etc. We're using the same solutions from the same bottles, etc. (we make all of our own solutions). We are unable to think of what could be causing the problem. Also, our slides are still really black when they are finished rinsing after the wiegert's step. Could this be causing it? I appreciate any advice. Thanks, Rita Angel, HT University of Cincinnati _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Thu Feb 12 16:59:07 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Critical Incident? Message-ID: Hi Lynda, I personally would think that the discrepancy in the initial alcohols would not cause that much of a problem, since they are followed by 3 absolutes (were these ok?) and xylene. If anything they would have caused an underprocessing problem and you said the tissues were "too dehydrated and brittle to be of any diagnostic use". It sounds to me to possibly be a heat problem rather than dehydration. Maybe there was a processor malfunction that just coincidentally happened at the same time. I think it would be best to withhold a reprimand until the cause is positively worked out. Maybe some sample tissues could be run with the erroneous alcohol percentages and see if the problem with the tissue is reproducible. I had a run of small tissues get fried on the tissue processor a few years ago (on a short 15 minute run) and it was just a variation of 1-2 degrees misread on the sensor on the paraffins. It was reading the proper temp on screen, but in reality, the paraffin temp was too high. They were as you said "too dehydrated and brittle to be of any diagnostic use". I couldn't save them even using the "mummified tissue retrieving" protocol and they were finally trashed. Overly dehydrated tissues can usually just be soaked longer on an ice tray, but once they are freedom fried, they are not salvageable. Hope this helps. Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "JENNIFER SCHUMACHER" 2/12/2004 2:44:08 PM >>> Unfortunately, I do not have an answer but another question to add . . . we have had two incidents in the last year in which we believe we had a contamination problem, and our tissues were mostly destroyed. Some samples had to be repeated, and our pathologists are determined to find someone to blame. Has this ever happened to anyone else? Were you able to pinpoint the problem, and how can you "guarantee" it will never happen again? All suggestions are welcome, offline is fine if you are not comfortable answering online. Thanks. Jennifer >>> Derek and/or Lynda Leopold 02/12/04 01:25PM >>> Hi Histonetters, I am hoping to get some opinion off-list from supervisors (and anyone else who may care to comment) on an event which happened recently in our lab. To be as short as possible, the person who was responsible for changing the solutions on our Tissue Tek 5 biopsy processor somehow (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of 95%), and the second alcohol to 88% (instead of 95%). For context, we do a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 changes of 95%, then 3 changes of 100% and on to xylene and paraffin. Now that you have the situation, I have two questions: 1) What sorts of changes, if any, would one expect in the biopsy tissues (endo, cervix, etc) exposed to this miscalculated regimen ? 2) If, as some of us surmise, this mistake in solution calculation could be linked to biopsy tissues that were too dehydrated and brittle to be of any diagnostic use, should that constitute a critical error/reportable mistake? We have apparently never experienced such terrible tissues in recent memory, and there is some debate as to whether the low-concentration alcohols could have been the cause. I look forward to your very expert opinions! Thanks Lynda Leopold Harrow, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RFail <@t> Charleston.net Thu Feb 12 16:54:52 2004 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] massons trichrome In-Reply-To: <5.1.0.14.2.20040212151129.00b69470@ucmail3.uc.edu> Message-ID: <000901c3f1bb$3a89e5c0$ab10a6a5@rena> Incomplete washing after Weigert's will cause the purple staining. And Make sure the Weigert's was correctly made. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rita Angel Sent: Thursday, February 12, 2004 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] massons trichrome hello all, I was hoping someone could help us with a trichrome problem. We are suddenly having problems with the muscle in heart tissue staining purple instead of red. Our skin control is staining the same way. There are 2 techs in the lab, and we're both having problems. The problems are with complete batches, not slides within the batch. We each stained 2 batches in a row, one batch stained with the purple muscle, and the 2nd batch with red muscle. We're leaving the slides in the solutions the same amount of time and rinsing the same amount of times, etc. We're using the same solutions from the same bottles, etc. (we make all of our own solutions). We are unable to think of what could be causing the problem. Also, our slides are still really black when they are finished rinsing after the wiegert's step. Could this be causing it? I appreciate any advice. Thanks, Rita Angel, HT University of Cincinnati _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From scoop <@t> mail.nih.gov Thu Feb 12 18:11:58 2004 From: scoop <@t> mail.nih.gov (Cooperman, Sharon (NIH/NICHD)) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] mouse perfusion Message-ID: <6000BB14AFA9A741BC2315A598837ED50C61F3DE@nihexchange4.nih.gov> Dear Histoneteers, I am having trouble with mouse perfusion. We do an intracardiac (left ventricle perfusion). I start out fine, but about halfway through the perfusion I get a bad hand cramp (I have writer's cramp) and can't hold the needle well. I often wind up punching through the septum or the anterior wall and the rest of the perfusion doesn't go well, so I get good flushing of blood but poor fixation. Does anyone know of a method for holding the needle without hand fatigue? There are people in my lab who don't have writer's cramp who have much less trouble with perfusions, but I can't change the writer's cramp stuff. Thanks, Sharon From j.browne <@t> auckland.ac.nz Thu Feb 12 18:06:43 2004 From: j.browne <@t> auckland.ac.nz (Jeremy Browne) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe me. thanks jeremy From cfavara <@t> niaid.nih.gov Thu Feb 12 18:24:43 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] mouse perfusion Message-ID: Sharon, I use a butterfly needle to perfuse, roll up some 4x4 gauze to tiny cylinders. Support the body of the mouse with the rolls of gauze and tape the needle with white surgical tape. You can perfuse to your hearts content-tee hee! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Cooperman, Sharon (NIH/NICHD) Sent: Thursday, February 12, 2004 5:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] mouse perfusion Dear Histoneteers, I am having trouble with mouse perfusion. We do an intracardiac (left ventricle perfusion). I start out fine, but about halfway through the perfusion I get a bad hand cramp (I have writer's cramp) and can't hold the needle well. I often wind up punching through the septum or the anterior wall and the rest of the perfusion doesn't go well, so I get good flushing of blood but poor fixation. Does anyone know of a method for holding the needle without hand fatigue? There are people in my lab who don't have writer's cramp who have much less trouble with perfusions, but I can't change the writer's cramp stuff. Thanks, Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From r.lederer <@t> uq.edu.au Fri Feb 13 00:58:05 2004 From: r.lederer <@t> uq.edu.au (Rose Lederer) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] antifeline pro-insulin antibody In-Reply-To: <20031119230900.17446.52503.Mailman@swlx167.swmed.edu> Message-ID: <000001c3f1fe$bb041520$89a6a8c0@vet.uq.edu.au> Sorry to abuse your expertise again, but I have been looking for pro-insulin antibodies and glucagon antibodies that work on a variety of mammals or best: on feline pancreas! Does anyone have experience with this /has used one that is still on the market? (Prof. Augstein had one proinsulin AB made in his lab 10 years ago, but I couldn't contact him so far about it) Thank you! Rose From aidanhisto <@t> yahoo.co.uk Fri Feb 13 03:54:26 2004 From: aidanhisto <@t> yahoo.co.uk (=?iso-8859-1?q?Aidan=20Schurr?=) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] IHC background stain In-Reply-To: Message-ID: <20040213095426.29276.qmail@web25204.mail.ukl.yahoo.com> Hi Roger, 5um does seem a little on the thick side, although if this is what you always have done, then obviously something else has changed. I tend to cut all my IHC slides at 3um... Other reasons for excessive background could be: * incorrect pH of buffer * antibody / detection system 'off'/expired (has it been left out on the bench too often?!) * sections dried out * new batch of antibody - may need retitering * Have you switched retrieval solutions? EDTA can give notiveable background on its own... These are just a few ideas - as you know, IHC is a bit of a black art, so your best bet to to look closely for any changes... Best of Luck Aidan "Charles, Roger" wrote: Hello all, I do all the IHC for our diagnostic veterinary laboratory. Latley i have been getting a lot of background stain from certain protocols. I also over heard one of the pathologists state the sections are getting thicker. Could thick section cause background stain on IHC? I checked my histologist microtome and it is set at 5um. Could her microtome need calibrated? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- BT Yahoo! Broadband - Free modem offer, sign up online today and save ?80 From AFeatherstone <@t> KaleidaHealth.Org Fri Feb 13 05:09:54 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] helicobactor pylori Message-ID: Our lab is doing a "gazillion" Warthin Starry's and I was wondering about an antibody for the ventana instead. Any suggestions about vendor and is it expensive? Annette Ht/MLT CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From AFeatherstone <@t> KaleidaHealth.Org Fri Feb 13 05:14:27 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Cutting 20-50 micron sections Message-ID: Any suggestions for cutting thick (20-50) micron paraffin sections. They curl when they hit the blade and though I am perservering, I was hoping for a tip or two, or three or four or five ................. Annette FeatherstoneHT/MLT Kaleida Health CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Barry.R.Rittman <@t> uth.tmc.edu Fri Feb 13 06:08:59 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Cutting 20-50 micron sections Message-ID: <566FB0B522443D43AF02D2ADBE35A6F063595E@UTHEVS3.mail.uthouston.edu> Annette Depending on your tissue, you may wish to consider embedding in a lower melting point paraffin wax. This will generally enable you to cut much thicker sections will less curling and cracking. You can lower the MP of paraffin wax with a variety of additives such as beeswax or buy a lower MP wax from the supplier. Other approaches would be to use a thin layer beeswax on the top and bottom of the block for better adherence between sections as they are being cut so that they remain flatter; or use a block of lower MP wax wiping a thin layer of this on the surface of the block between sections. The latter is very time consuming and you have to be careful to be squeaky clean but it helps especially with sections that tend to crack. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Featherstone, Annette Sent: Fri 2/13/2004 5:14 AM To: Histonet (E-mail) Cc: Subject: [Histonet] Cutting 20-50 micron sections Any suggestions for cutting thick (20-50) micron paraffin sections. They curl when they hit the blade and though I am perservering, I was hoping for a tip or two, or three or four or five ................. Annette FeatherstoneHT/MLT Kaleida Health CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Fri Feb 13 06:56:23 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] helicobactor pylori Message-ID: To all, In reference to warthin starry- if you are looking for h pylori there are good antibodies for camplobactor. I don't know if any are prediluted for the bar coded ventana, but if you have the new one, they say you can use your own antibodies. >>> "Featherstone, Annette" 02/13/04 06:09AM >>> Our lab is doing a "gazillion" Warthin Starry's and I was wondering about an antibody for the ventana instead. Any suggestions about vendor and is it expensive? Annette Ht/MLT CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Salvacion.DeLovino <@t> cshs.org Fri Feb 13 06:56:13 2004 From: Salvacion.DeLovino <@t> cshs.org (DeLovino, Salvacion S.) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Cutting 20-50 micron sections Message-ID: Try putting the blocks to be cut in warm water (your water bath will do) for a few seconds right before cutting. Never put them on ice. Don't use new blades. Good luck........ Salvacion S. Delovino -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Featherstone, Annette Sent: Friday, February 13, 2004 3:14 AM To: Histonet (E-mail) Subject: [Histonet] Cutting 20-50 micron sections Any suggestions for cutting thick (20-50) micron paraffin sections. They curl when they hit the blade and though I am perservering, I was hoping for a tip or two, or three or four or five ................. Annette FeatherstoneHT/MLT Kaleida Health CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Warning: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 -- and destroy the related message. Thank you for your cooperation. From ROENN <@t> surgery.wisc.edu Fri Feb 13 08:13:07 2004 From: ROENN <@t> surgery.wisc.edu (Drew Allan Roenneburg) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] helicobactor pylori Message-ID: Hi Annette, Instead of doing the warthin-starry or IHC you can try doing an Alcian yellow, toluidine blue. It's fast and gives good results. It doesn't stain all spirochetes though. Drew From coyne <@t> tpc.tulane.edu Fri Feb 13 09:19:49 2004 From: coyne <@t> tpc.tulane.edu (Carol Coyne) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] cutting 20-50 micron sections Message-ID: <5.2.0.9.0.20040213091338.00a3aec0@rhesus.tpc.tulane.edu> I've had the best results cutting 25 micron sections with the blade angle almost straight up. (@6 on the microm) The sections were at room temp. and embedded in paraplast plus paraffin. From mcauliff <@t> umdnj.edu Fri Feb 13 11:50:58 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] mouse perfusion In-Reply-To: <6000BB14AFA9A741BC2315A598837ED50C61F3DE@nihexchange4.nih.gov> References: <6000BB14AFA9A741BC2315A598837ED50C61F3DE@nihexchange4.nih.gov> Message-ID: <402D0E82.8000906@umdnj.edu> Hi Sharon: You may be able to shorten the time of perfusion and avoid hand cramps by reducing the amount of washout solution. In my experience many people spend way too much time and solution washing blood out of the circulatory system. 10 ml of buffer or saline is plenty to wash the blood out of a mouse. After that it is on to fixative. You will never get all of the blood out of every vessel so why try. Geoff Cooperman, Sharon (NIH/NICHD) wrote: >Dear Histoneteers, > >I am having trouble with mouse perfusion. We do an intracardiac (left >ventricle perfusion). I start out fine, but about halfway through the >perfusion I get a bad hand cramp (I have writer's cramp) and can't hold the >needle well. I often wind up punching through the septum or the anterior >wall and the rest of the perfusion doesn't go well, so I get good flushing >of blood but poor fixation. Does anyone know of a method for holding the >needle without hand fatigue? There are people in my lab who don't have >writer's cramp who have much less trouble with perfusions, but I can't >change the writer's cramp stuff. > >Thanks, >Sharon > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Fri Feb 13 08:54:40 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] massons trichrome In-Reply-To: <000901c3f1bb$3a89e5c0$ab10a6a5@rena> References: <5.1.0.14.2.20040212151129.00b69470@ucmail3.uc.edu> Message-ID: <3.0.6.32.20040213075440.00be8db0@gemini.msu.montana.edu> The Weigerts may also be old - we made up our Weigerts fresh each time since the oxidizer continues to do just that, oxidize the hematoxylin until it no longer stains properly. This is explained in Sheehan and Hrapchak book, even though some people use their Weigerts longer, we never took the chance. At 05:54 PM 2/12/2004 -0500, you wrote: >Incomplete washing after Weigert's will cause the purple staining. And >Make sure the Weigert's was correctly made. >Rena Fail > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From kemlo <@t> tiscali.co.uk Fri Feb 13 09:12:08 2004 From: kemlo <@t> tiscali.co.uk (Kemlo Rogerson) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Critical Incident? In-Reply-To: Message-ID: <000c01c3f243$c2e67680$bf362850@KEMLOS> Define contamination? What with? Destroyed? How and by what? Blame? Why? We are a no blame culture and anyway the system should catch these problems Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 0208 970 8414 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts: kemlo@tiscali.co.uk or kemlo1@btinternet.com Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JENNIFER SCHUMACHER Sent: 12 February 2004 20:44 To: histonet@lists.utsouthwestern.edu; leopold@mnsi.net Subject: Re: [Histonet] Critical Incident? Unfortunately, I do not have an answer but another question to add . . . we have had two incidents in the last year in which we believe we had a contamination problem, and our tissues were mostly destroyed. Some samples had to be repeated, and our pathologists are determined to find someone to blame. Has this ever happened to anyone else? Were you able to pinpoint the problem, and how can you "guarantee" it will never happen again? All suggestions are welcome, offline is fine if you are not comfortable answering online. Thanks. Jennifer >>> Derek and/or Lynda Leopold 02/12/04 01:25PM >>> Hi Histonetters, I am hoping to get some opinion off-list from supervisors (and anyone else who may care to comment) on an event which happened recently in our lab. To be as short as possible, the person who was responsible for changing the solutions on our Tissue Tek 5 biopsy processor somehow (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of 95%), and the second alcohol to 88% (instead of 95%). For context, we do a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 changes of 95%, then 3 changes of 100% and on to xylene and paraffin. Now that you have the situation, I have two questions: 1) What sorts of changes, if any, would one expect in the biopsy tissues (endo, cervix, etc) exposed to this miscalculated regimen ? 2) If, as some of us surmise, this mistake in solution calculation could be linked to biopsy tissues that were too dehydrated and brittle to be of any diagnostic use, should that constitute a critical error/reportable mistake? We have apparently never experienced such terrible tissues in recent memory, and there is some debate as to whether the low-concentration alcohols could have been the cause. I look forward to your very expert opinions! Thanks Lynda Leopold Harrow, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Fri Feb 13 09:33:42 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Fli-1 antibody Message-ID: Insight Biotechnology PO Box 520. Wembley, Middlesex 0208 3850308 -----Original Message----- From: Steve Machin UK [mailto:stevemachinuk@yahoo.co.uk] Sent: 12 February 2004 12:15 To: Histonet Histonet Histonet Subject: [Histonet] Fli-1 antibody Does anyone know of a UK supplier of Fli-1. I understand that Santa Cruz does it but I dont have a UK supplier for them. Best Wishes Steve Machin UK ___________________________________________________________ BT Yahoo! Broadband - Free modem offer, sign up online today and save ?80 http://btyahoo.yahoo.co.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Auld <@t> whnt.nhs.uk Fri Feb 13 09:46:18 2004 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Re critical incident Message-ID: Lynda How can you overdehydrate? excessive times in alcohol and xylene will harden tissue but this is not what happened in your case. I would not have thought that the increase in concentrations would have made any difference to your tissues. It is certainly worth recording somewhere what happened in case it reoccurs. John John Auld FIBMS MSc Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral U.K. Tel 0151 604 7025 Message: 3 Date: Thu, 12 Feb 2004 14:25:54 -0500 From: Derek and/or Lynda Leopold Subject: [Histonet] Critical Incident? To: histonet@lists.utsouthwestern.edu Message-ID: <402BD342.4040201@mnsi.net> Content-Type: text/plain; charset=us-ascii; format=flowed Hi Histonetters, I am hoping to get some opinion off-list from supervisors (and anyone else who may care to comment) on an event which happened recently in our lab. To be as short as possible, the person who was responsible for changing the solutions on our Tissue Tek 5 biopsy processor somehow (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of 95%), and the second alcohol to 88% (instead of 95%). For context, we do a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 changes of 95%, then 3 changes of 100% and on to xylene and paraffin. Now that you have the situation, I have two questions: 1) What sorts of changes, if any, would one expect in the biopsy tissues (endo, cervix, etc) exposed to this miscalculated regimen ? 2) If, as some of us surmise, this mistake in solution calculation could be linked to biopsy tissues that were too dehydrated and brittle to be of any diagnostic use, should that constitute a critical error/reportable mistake? We have apparently never experienced such terrible tissues in recent memory, and there is some debate as to whether the low-concentration alcohols could have been the cause. I look forward to your very expert opinions! Thanks Lynda Leopold Harrow, Ontario From pruegg <@t> colobio.com Fri Feb 13 09:58:31 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] von Willebrand factor IHC in rat kidneys In-Reply-To: Message-ID: Antje, I too notice the different staining intensity for factor 8 in different organs. I do use proteinase K from DAKO for 5 min. instead of trypsin, you might also try enhancing the DAB reaction with something like copper sulfate. Perhaps there is a difference of expression of the protein in kidney cells. I see the strongest F8 expression in lung. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of antje.marcantonio@pharma.novartis.com Sent: Thursday, February 12, 2004 1:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] von Willebrand factor IHC in rat kidneys Hello, I'm dealing with a strange phenomena. I have a nice working protocol for vWf in rat FFPE lung or heart, including a 15 min pretreatment of trypsin, 1 hour incubation of primary antibody (Dako) 1:500 followed by Envision/HRP. I get constantly strong and clean staining. The same protocol used for kidneys (yes, fixed and processed with the exactly same schedule) shows only weak staining in the vessels and very weak signal in the glomeruli. I tried to use the primary more concentrated (up to 1:100) and to prolong the its incubation to 2 hours with only little improvement. Any ideas ? Should I consider another enzyme for pretreatment ? Longer digestion with trypsin simply increased the background ,not the signal. Is there any explanation why a protocol which works nicely in lung and heart is not applicable for the kidneys? Thanks to everybody, Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Feb 13 09:59:45 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] antifeline pro-insulin antibody References: <000001c3f1fe$bb041520$89a6a8c0@vet.uq.edu.au> Message-ID: <001601c3f24a$667d5f60$78065486@vdl220FAC> DAKO's anti-glucagon works on all the mammals I've tried it on. (Its anti-insulin also works fine.) Jan Shivers U of MN Vet Diag Lab ----- Original Message ----- From: "Rose Lederer" To: Sent: Friday, February 13, 2004 12:58 AM Subject: [Histonet] antifeline pro-insulin antibody > Sorry to abuse your expertise again, > > but I have been looking for pro-insulin antibodies and glucagon > antibodies that work on a variety of mammals or best: on feline > pancreas! > Does anyone have experience with this /has used one that is still on the > market? > > (Prof. Augstein had one proinsulin AB made in his lab 10 years ago, > but I couldn't contact him so far about it) > > Thank you! > Rose > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mark.lewis <@t> thermo.com Fri Feb 13 10:09:16 2004 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Monitoring Xylene Substitutes Message-ID: Does anyone have any information as to whether or not Xylene substitute exposure needs to be monitored like Xylene does ? In particular Aliphatic Hydrocarbons... Thanks ! Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com From leopold <@t> mnsi.net Fri Feb 13 10:09:41 2004 From: leopold <@t> mnsi.net (Derek and/or Lynda Leopold) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Thanks for responses re Critical Incident Message-ID: <402CF6C5.70401@mnsi.net> Hi All, Just wanted to publicly thank all who sent me some words of advice. My original thought was that jumping from 88% to 3 changes of absolute might have caused the dehydration to happen too rapidly and therefore cause the shrinkage we saw, but it seems that the more likely reason was under-infiltration of paraffin. We are a lab of fairly inexperienced staff, due to a couple of retirements happening at the same time, so we are learning VERY quickly!! This list is an incredible resource for us; we discuss the digests nearly every day. So, I just want to head off any more tying up the netspace with what seemed like an obvious answer (to everyone but me, ha ha.); you can still send me info off-list if you want. Thanks again!! Lynda From shive003 <@t> umn.edu Fri Feb 13 10:56:14 2004 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] von Willebrand factor IHC in rat kidneys References: Message-ID: <006001c3f252$4a4b8850$78065486@vdl220FAC> Antje, I have also noticed decreased staining intensity with vWF in kidney glomeruli, compared to other sites. I use DAKO's product with Proteinase K enzyme digestion for 5 minutes at room temperature, primary antibody at 1:600 for 30 minutes, LSAB2 detection system, AEC chromogen. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Laboratory USA ----- Original Message ----- From: To: Sent: Thursday, February 12, 2004 2:45 AM Subject: [Histonet] von Willebrand factor IHC in rat kidneys > Hello, > > I'm dealing with a strange phenomena. > I have a nice working protocol for vWf in rat FFPE lung or heart, including a 15 min pretreatment of trypsin, 1 hour incubation of primary > antibody (Dako) 1:500 > followed by Envision/HRP. I get constantly strong and clean staining. > The same protocol used for kidneys (yes, fixed and processed with the > exactly same schedule) shows only weak staining in the vessels and very > weak signal in the glomeruli. > I tried to use the primary more concentrated (up to 1:100) and to prolong > the its incubation to 2 hours with only little improvement. > > Any ideas ? Should I consider another enzyme for pretreatment ? Longer > digestion with trypsin simply increased the background ,not the signal. > Is there any explanation why a protocol which works nicely in lung and > heart is not applicable for the kidneys? > > Thanks to everybody, > > Antje Marcantonio > Novartis Pharma AG > BU Transplantation Research > Basel, Switzerland > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Bujakbarb <@t> aol.com Fri Feb 13 11:18:40 2004 From: Bujakbarb <@t> aol.com (Bujakbarb@aol.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Re: Histonet Digest, Vol 3, Issue 16 Message-ID: <3C317B86.175B65F8.0234048C@aol.com> Please change my email address to the following: barbara.bujak@pharma.novartis.com If I should contact someone else about this request please let me know. Thank you Barbara Bujak From gentras <@t> vetmed.auburn.edu Fri Feb 13 10:25:41 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:34 2005 Subject: Fwd: [Histonet] Critical Incident? Message-ID: <6.0.1.1.0.20040213101058.0257b590@mailhost.vetmed.auburn.edu> Hello, I would suggest you rehydrate and process using appropriate solutions. The rehydration procedure that works well for me is from the Sheehan/Mosby 2nd or 3rd edition of Theory and Practice of Histotechnology. Please see as follows: place tissue in following solution overnight: 0.6g Sodium Carbonate, 42ml distilled H2O, 18m Absolute alcohol, and process the following day on a standard procedure, starting in 80% alcohol. Hope this helps. Atoska >Date: Thu, 12 Feb 2004 14:25:54 -0500 >From: Derek and/or Lynda Leopold >User-Agent: Mozilla/5.0 (Windows; U; Win98; en-US; > rv:1.0.2) Gecko/20030208 Netscape/7.02 >X-Accept-Language: en-us, en >To: histonet@lists.utsouthwestern.edu >X-Scan-Signature: ff0f5448b767a158ef77d41af1533689 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: cf68ae42b5c1cad19b735e8c35ea8abb >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] Critical Incident? >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.1 required=6.5 tests=RCVD_IN_SORBS autolearn=no > version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes > >Hi Histonetters, > I am hoping to get some opinion off-list from supervisors (and anyone > else who may care to comment) on an event which happened recently in our > lab. To be as short as possible, the person who was responsible for > changing the solutions on our Tissue Tek 5 biopsy processor somehow > (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of > 95%), and the second alcohol to 88% (instead of 95%). For context, we do > a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 > changes of 95%, then 3 changes of 100% and on to xylene and > paraffin. Now that you have the situation, I have two questions: 1) What > sorts of changes, if any, would one expect in the biopsy tissues (endo, > cervix, etc) exposed to this miscalculated regimen ? 2) If, as some of > us surmise, this mistake in solution calculation could be linked to > biopsy tissues that were too dehydrated and brittle to be of any > diagnostic use, should that constitute a critical error/reportable mistake? > We have apparently never experienced such terrible tissues in recent > memory, and there is some debate as to whether the low-concentration > alcohols could have been the cause. >I look forward to your very expert opinions! >Thanks >Lynda Leopold >Harrow, Ontario > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From WesterM <@t> MedImmune.com Fri Feb 13 14:55:05 2004 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] RE: Plastic laminate countertops Message-ID: <83899F0EC7671543B305FB5694024DE6BAD2B1@medimmune4.medimmune.com> I worked in a Histo lab where we had white laminate countertops! They weren't white for long! Other than the color issues they seemed to hold up just fine, even against occasional xylene spills, etc. Good Luck, Martha S. Wester Associate Scientist MedImmune, Inc. Gaithersburg, MD 20878 (240) 632-4794 From nperson211 <@t> comcast.net Fri Feb 13 15:28:15 2004 From: nperson211 <@t> comcast.net (nperson211@comcast.net) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Protein Kinase antibodies Message-ID: <021320042128.12042.680e@comcast.net> Hello, Does anyone have any experience with antibodies for Protein Kinase C? Help!! Nancy Lemke Hermelin Brain Tumor Center Henry Ford Hospital Detroit, MI p From shahina <@t> dcnet2000.com Fri Feb 13 18:19:37 2004 From: shahina <@t> dcnet2000.com (shahina sobhan) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Retic Stain Kit Message-ID: <001801c3f290$58e1a0a0$9490fea9@w7z6v8> Hi Could anybody share their exprience of using commercially made retic stain kit, we have been using it for a while but without any good result,pathologists as well as the techs are not at all happy with the staining results. The company is Poly Scientific. Thanks to all in advance . From gudrun.lang <@t> aon.at Sat Feb 14 03:34:19 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] nexes stainer Message-ID: <006801c3f2dd$b88eb400$eeeea8c0@SERVER> A question to those, who work with the nexes special stainer: We are going to test the nexes. We have max. 20 slides to stain per day. For example 8 PAS, 3 CAB (trichrom), 3 NASD, 3 BB, 2 Retic., 3 Jones-Methenamin and so on. And some of the stains can't be performed on the nexes. Doing it by hand we need most of the time about 1 to 1,5 hours in the morning. Can you give me an advice, what amount of stains per day makes sense to use an automatic stainer like the nexes? And how long do you need from preparing the slides until the ready stains.? Thanks in advance Gudrun Lang, Austria From Andrew.Gray <@t> vcp.monash.edu.au Sat Feb 14 05:48:10 2004 From: Andrew.Gray <@t> vcp.monash.edu.au (Andrew Gray) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Re: mouse perfusion References: Message-ID: <203.217.48.247.1076758756.98223@my.monash.edu.au> Hi Sharon, I do perfusions on rats, and I place a surgical clamp over the heart to fix the needle (blunt, 18 gauge) in place. The clamp I use resembles this: http://www.edmo.com/s14/42H.asp , but has a curved nose. The needle is attached to a tube supplying the solutions; this keeps the liquid reservoirs well out of the way. I use an electrical peristaltic pump to push the liquids through. Best wishes Andrew Gray PhD student From wayneholland1959 <@t> msn.com Sat Feb 14 13:25:22 2004 From: wayneholland1959 <@t> msn.com (Wayne Holland) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] H&E Prep Only Cost?? (Survey) Message-ID: I have all the figures for the actual cost of getting the job done. But can you all, the laboratories that do povide such a service, tell me what the current charge is for an H&E prep? What I mean is from specimen bottle to finished slide, "period". This does not include any interpretation. Your input would be extremely helpful, once again thanks much. _________________________________________________________________ Let the advanced features & services of MSN Internet Software maximize your online time. http://click.atdmt.com/AVE/go/onm00200363ave/direct/01/ From jstaruk <@t> masshistology.com Sat Feb 14 15:51:47 2004 From: jstaruk <@t> masshistology.com (Mass Histology Service) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] H&E Prep Only Cost?? (Survey) In-Reply-To: Message-ID: It depends on how the tissue is submitted. Basically, the more work you do in preparing the tissue, the cheaper it is for an H&E-stained slide. Check out our homepage for a detailed pricelist. Jim ____________________________ James E. Staruk, HT(ASCP) Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wayne Holland Sent: Saturday, February 14, 2004 2:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Prep Only Cost?? (Survey) I have all the figures for the actual cost of getting the job done. But can you all, the laboratories that do povide such a service, tell me what the current charge is for an H&E prep? What I mean is from specimen bottle to finished slide, "period". This does not include any interpretation. Your input would be extremely helpful, once again thanks much. _________________________________________________________________ Let the advanced features & services of MSN Internet Software maximize your online time. http://click.atdmt.com/AVE/go/onm00200363ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gudrun.lang <@t> aon.at Sun Feb 15 03:46:50 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] nexes stainer References: Message-ID: <000a01c3f3a8$a2938ce0$eeeea8c0@SERVER> Mary, BB is the german abbriviation for "Berliner Blau", that is prussian blue in English. Do you think, it is saving of time to do part of the stains on the nexes and the others by hand? Are the advantages of a standardized stain so big in spite of doing all by hand? Gudrun ----- Original Message ----- From: MARY T HODGES To: gudrun.lang@aon.at Sent: Saturday, February 14, 2004 10:56 PM Subject: RE: [Histonet] nexes stainer Gudrun, I think bb you mean brown and brinm. I would do pas,tri,retic joneson the stainer.do as many as possible on the stainer. The reproducablity is great.I would only do the stains that the instrument would not do by hand. Thanks Tere Hodges >From: "Gudrun Lang" >To: "Histonetliste" >Subject: [Histonet] nexes stainer >Date: Sat, 14 Feb 2004 10:34:19 +0100 > >A question to those, who work with the nexes special stainer: >We are going to test the nexes. We have max. 20 slides to stain per day. For example 8 PAS, 3 CAB (trichrom), 3 NASD, 3 BB, 2 Retic., 3 Jones-Methenamin and so on. >And some of the stains can't be performed on the nexes. >Doing it by hand we need most of the time about 1 to 1,5 hours in the morning. > >Can you give me an advice, what amount of stains per day makes sense to use an automatic stainer like the nexes? >And how long do you need from preparing the slides until the ready stains.? > >Thanks in advance >Gudrun Lang, Austria >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Click here for a FREE online computer virus scan from McAfee. From a-lisowski <@t> northwestern.edu Sun Feb 15 08:27:30 2004 From: a-lisowski <@t> northwestern.edu (a-lisowski@northwestern.edu) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Varistain wells? Message-ID: <200402151427.i1FERXpt006794@hecky.it.northwestern.edu> Hi! Does anyone have a varistain staining well willing to give away a sell? Thanks Andrew Lisowski a-lisowski@northwestern.edu From a-lisowski <@t> northwestern.edu Sun Feb 15 08:27:35 2004 From: a-lisowski <@t> northwestern.edu (a-lisowski@northwestern.edu) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Varistain wells? Message-ID: <200402151427.i1FERhWi006986@hecky.it.northwestern.edu> Hi! Does anyone have a varistain staining well willing to give away a sell? Thanks Andrew Lisowski a-lisowski@northwestern.edu From becky.garrison <@t> jax.ufl.edu Sun Feb 15 13:28:56 2004 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Turn around times Message-ID: <38F928258973D711977200D0B7DB9D74018DE3FB@mail.umc.ufl.edu> Question about TAT statistics and how they are compiled. To meet the CAP guidelines of a 2 working day TAT for routine surgical path specimens, do other LIS systems have a program which eliminates those cases with special stains, IHC's, decals, levels, recuts prolonged fixation, etc.and report a TAT of routine cases that are signed out based on original H&E only? or are all cases included and notations made of numbers of cases that may cause a variance from the 2 working day TAT? I am interested to know how other departments pull data for TAT statistics. We currently separate biopsies from non-biopsies and run TAT's for each. However, add on procedures are included in and affect the final TAT. Also weekends are included in the final numbers. Our LIS is HBOC and has been in use 9+ ish years. The TAT table is from the clinical module and makes no allowances for add on procedures. Are there HBO users out there that can share how they generate Anatomic TAT statistics? Any other LIS users that can share how the data is pulled? Thanks in advance for any and all input. Becky Garrison Pathology Suprevisor Shands Jacksonville Jacksonville, FL 32209 From georgecole <@t> ev1.net Sun Feb 15 14:55:38 2004 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] special videos Message-ID: <000001c3f406$1190a8a0$0a4dbad0@hppav> Histotechs who have received the muscle and nerve packet. Several of you histotechs out there that have asked for the muscle and nerve packets have talked about showing the videos to others. After all the practicing it took to make a video for a new comer like me to computers, I was glad to get the video together. But after a bit of working with the discs, I noticed several shots were not as clear and darker in the video than in the digital tape that was fed to the computer to make the video. It turns out there is quite a compromise in quality getting motion pictures from tape to discs. And any enlargement of the video pictures is wavy and not of the same clarity and brightness as a smaller version of the scene. So, I have come to see that sharing the video with any kind of audience will not be very good to look at. I wish to offer to any of you that have received a video, if you wish to use it for showing to any kind of group that requires enlarged projection onto a screen I will be glad to send you a VHS or digital 8 tape of the video much better suited to showing on a larger screen for more than one person at a time. This will also be without charge. Let me know what form you can use. georgecole@ev1.net From caroline.stott <@t> anatomy.otago.ac.nz Sun Feb 15 15:37:09 2004 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] IHC background stain Message-ID: <5.2.1.1.0.20040216103326.0264c800@anatomy.otago.ac.nz> Hi Roger, I don't think the 5 micron sections should be too much of a problem. We cut a lot of immuno sections at 7 microns and they come up with great results. Are you using a polyclonal antibody? We had trouble with background staining when using them. It might be a good idea to calibrate the microtome though. Caroline Hi Roger, 5um does seem a little on the thick side, although if this is what you always have done, then obviously something else has changed. I tend to cut all my IHC slides at 3um... Other reasons for excessive background could be: * incorrect pH of buffer * antibody / detection system 'off'/expired (has it been left out on the bench too often?!) * sections dried out * new batch of antibody - may need retitering * Have you switched retrieval solutions? EDTA can give notiveable background on its own... These are just a few ideas - as you know, IHC is a bit of a black art, so your best bet to to look closely for any changes... Best of Luck Aidan "Charles, Roger" wrote: Hello all, I do all the IHC for our diagnostic veterinary laboratory. Latley i have been getting a lot of background stain from certain protocols. I also over heard one of the pathologists state the sections are getting thicker. Could thick section cause background stain on IHC? I checked my histologist microtome and it is set at 5um. Could her microtome need calibrated? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 V> Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From cwscouten <@t> myneurolab.com Sun Feb 15 16:54:07 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] mouse perfusion Message-ID: In the rat, I snip off the bottom of the heart, and insert a gavage needle (a feeding needle, with a round or oval ball on the end) up through the left ventricle into the ascending aorta. You can't miss, it channels you there. And no chance of the round ball penetrating out. Then put a clamp (forceps?) on the aorta behind the ball, and remove your hands from the area. No need to hold the needle as perfusion progresses. I have no reason to think this won't work in mice as well. The Perfusion One apparatus comes with a set of gavage needles suitable for mice, rats, or larger rodents. See the following link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21 You can buy the needles separately. They are listed at the website. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooperman, Sharon (NIH/NICHD) Sent: Thursday, February 12, 2004 6:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] mouse perfusion Dear Histoneteers, I am having trouble with mouse perfusion. We do an intracardiac (left ventricle perfusion). I start out fine, but about halfway through the perfusion I get a bad hand cramp (I have writer's cramp) and can't hold the needle well. I often wind up punching through the septum or the anterior wall and the rest of the perfusion doesn't go well, so I get good flushing of blood but poor fixation. Does anyone know of a method for holding the needle without hand fatigue? There are people in my lab who don't have writer's cramp who have much less trouble with perfusions, but I can't change the writer's cramp stuff. Thanks, Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Sun Feb 15 16:58:08 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] mouse perfusion Message-ID: You can get ALL the blood out, if sufficient pressure is used. It is not about time or volume of fluid, but about pressure. See the pictures at the following link. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, February 13, 2004 11:51 AM To: Cooperman, Sharon (NIH/NICHD) Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] mouse perfusion Hi Sharon: You may be able to shorten the time of perfusion and avoid hand cramps by reducing the amount of washout solution. In my experience many people spend way too much time and solution washing blood out of the circulatory system. 10 ml of buffer or saline is plenty to wash the blood out of a mouse. After that it is on to fixative. You will never get all of the blood out of every vessel so why try. Geoff Cooperman, Sharon (NIH/NICHD) wrote: >Dear Histoneteers, > >I am having trouble with mouse perfusion. We do an intracardiac (left >ventricle perfusion). I start out fine, but about halfway through the >perfusion I get a bad hand cramp (I have writer's cramp) and can't hold >the needle well. I often wind up punching through the septum or the >anterior wall and the rest of the perfusion doesn't go well, so I get >good flushing of blood but poor fixation. Does anyone know of a method >for holding the needle without hand fatigue? There are people in my >lab who don't have writer's cramp who have much less trouble with >perfusions, but I can't change the writer's cramp stuff. > >Thanks, >Sharon > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From antje.marcantonio <@t> pharma.novartis.com Mon Feb 16 02:21:54 2004 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] thanks a lot - von Willebrand factor IHC in rat kidneys Message-ID: Thank you so much for all your inputs to Patsy, Jan, George, Nidal, Jeff, Tim and Nancy !!! You confirm my experiences. I will do some more trials. Soon I will let you know about further insights. Histonet - what a valuable forum ! Have a nice day. Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland From Conny.Gysemans <@t> med.kuleuven.ac.be Mon Feb 16 03:55:10 2004 From: Conny.Gysemans <@t> med.kuleuven.ac.be (Conny Gysemans) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] help Message-ID: <6.0.0.22.0.20040216105436.01b08fd0@u0024364.kuleuven.be> Dear histonetters, We are a small lab in Belgium trying to start with laser capture microdissection on pancreatic tissue. We would use the Leica LMD system which uses mounting of sections on frame foil membranes (membranes sealed around the edges with nail varnish). We would like to use Mayers' hematoxylin - eosin staining to identify both infiltrating immune cells and endocrine cells in the pancreatic islets. Can anyone give us some ideas on how to proceed? 1. preparation of tissue (fixation?) 2. applying specimen on slides (can tissue adhesive be used if we want to do RNA extraction thereafter?) 3. which protocol for staining? 30 sec 100% ethonol (3x) 30 sec 96% ethanol (1x) 30 sec 70% ethanol (1x) 30 sec distilled water (1x) 1 min hematoxylin rinse in water 1 min eosine rinse in water (remove excess water by placing a filter on the sample) 30 min air dry at 37?C 4. best protocol for RNA extraction? RNeasy Mini kit from QIAGEN? All suggestions and in-house knowledges are welcome..... Best regards From bernaweston <@t> hotmail.com Mon Feb 16 07:25:44 2004 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Loss of Nuclear Detail in Bone Marrow Message-ID: Our bone marrow latey are losing their nuclear detail, both in the core and aspirate. They are processed in 2 different processors as 2 different fixatives are used. Histochoice for the aspirate and B+ for the core. Our B+ cores have been coming our beautifully but the last few have developed solid blue nuclei with no chromatin detail. The aspirate goes immediately into Histochoice, the core is in B+ for 2 and half hours, in Decal Stat until they float, 15 minutes to an hour. The program is a 13 overnight process. Bernadette Weston HT Barberton Citizens Hospital Barberton, OH _________________________________________________________________ Choose now from 4 levels of MSN Hotmail Extra Storage - no more account overload! http://click.atdmt.com/AVE/go/onm00200362ave/direct/01/ From HOOVER_JENNIFER <@t> LILLY.COM Mon Feb 16 07:48:22 2004 From: HOOVER_JENNIFER <@t> LILLY.COM (Jennifer Hoover) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Storage of Primary Antibodies! Message-ID: Histonet, I have a question regarding the storage (short or longer term) of primary antibodies. Is it conducive to aliquot and store primary antibodies at -80C?? Is there the potential for diminished activity after storing the antibody for several weeks to several months? The aliquots that were used were thawed and used for one day only. Any volume that was not used was disgarded so freeze/thawing is not the problem. I would welcome any insight. Thank you very much! Jennifer Hoover BI Research Eli Lilly and Company From daniel.eberhard <@t> uni-bielefeld.de Mon Feb 16 08:12:37 2004 From: daniel.eberhard <@t> uni-bielefeld.de (DANIEL EBERHARD) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] apoptosis control tissue Message-ID: <4030CFD5.7060904@UNI-BIELEFELD.DE> Dear Histo-Listers, I would like to check some transgenic mouse tissues for apoptotic activities. Thus I?m looking for nice control tissues in which apoptosis is frequent - e.g . I thought of intestinal epithelium (cypts and villi). Can you recommend me other tissues/what you do use? Any suggestions would be helpful, Thanks in advance Daniel. -- (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V= Developmental Biology & Molecular Pathology Graduate Programe on Pattern Formation University of Bielefeld D 33501 Bielefeld/Germany FAX: xx49(0)521-106-5654 (-)-(-) --------------------------- \"/ --- =V= From HOOVER_JENNIFER <@t> LILLY.COM Mon Feb 16 08:41:46 2004 From: HOOVER_JENNIFER <@t> LILLY.COM (Jennifer Hoover) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Storage of Primary Antibodies! Message-ID: Hi Pam, Thank you for the timely reply. This particular antibody is an "in-house" antibody so I cannot readily give out the clone information. My reasoning for aliquoting and freezing was my concern that there are no preservatives (like sodium azide) added. However, I conducted one titration experiment with beautiful results. Then it was about 2 1/2 to 3 weeks later until I could conduct the complete experiment with all time points included. At this time I started having problems with the primary antibody dilutions. I suspect that the antibody does not like being frozen. I am using the same procedure and all the same reagents. Jennifer Hoover BI Research Eli Lilly and Company Pamela Marcum 02/16/2004 09:22 AM To: Jennifer Hoover cc: Subject: RE: [Histonet] Storage of Primary Antibodies! Hi Jennifer, Some antibodies are fine for storage at -80 and some are not. I think this will depend on the antibody. It may be useful to let us now which antibodies are not doing well as other people may have had experience with them and have suggestions for you. Thanks, Pam Marcum? > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer > Hoover > Sent: Monday, February 16, 2004 8:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Storage of Primary Antibodies! > > > Histonet, > > I have a question regarding the storage (short or longer > term) of primary antibodies. Is it conducive to aliquot and store primary > antibodies at -80C?? Is there the potential for diminished activity after > storing the antibody for several weeks to several months? The aliquots > that were used were thawed and used for one day only. Any volume that was > not used was disgarded so freeze/thawing is not the problem. I would > welcome any insight. Thank you very much! > > Jennifer Hoover > BI Research > Eli Lilly and Company > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcline <@t> wchsys.org Mon Feb 16 09:18:18 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] nexes stainer References: <006801c3f2dd$b88eb400$eeeea8c0@SERVER> Message-ID: <001101c3f4a0$1be45780$1d2a14ac@wchsys.org> I use the Ventana, we perform several different stains at a time, but there is a problem of not being able to run silver stains along with others because you run out of space for the stain vials. I find that even though we sometimes run the Ventana 2 - 3 times a day, it saves by not having all these stains done by hand, which frees my techs to perform other areas of the job. The quality is excellant, sometimes you have to tweek the stain to satisfy your paths. ----- Original Message ----- From: "Gudrun Lang" To: "Histonetliste" Sent: Saturday, February 14, 2004 4:34 AM Subject: [Histonet] nexes stainer A question to those, who work with the nexes special stainer: We are going to test the nexes. We have max. 20 slides to stain per day. For example 8 PAS, 3 CAB (trichrom), 3 NASD, 3 BB, 2 Retic., 3 Jones-Methenamin and so on. And some of the stains can't be performed on the nexes. Doing it by hand we need most of the time about 1 to 1,5 hours in the morning. Can you give me an advice, what amount of stains per day makes sense to use an automatic stainer like the nexes? And how long do you need from preparing the slides until the ready stains.? Thanks in advance Gudrun Lang, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pam <@t> ategra.com Mon Feb 16 09:59:11 2004 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Happy Valentines Day From Pam Message-ID: Happy Valentines Day, Histonetters ! I've attached a Valentines Day card (as a jpeg file). As well, here is the latest update on opportunities with some of my best clients throughout the US who are seeking Histology Supervisors, Histo Technologists and Histo Technicians. These are positions as direct employees of our client. There are fulltime 40 hour per week positions.. As a direct employee of one of our clients you will be provided with full benefits including Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative sign-on bonus. I have part time and temporary positions as well. I have supervisory, team lead and bench positions. These positions require HTL or HT certification or registry eligibility. Here are my NEW openings: 1. Indiana - Histo Tech 2. Nevada - Histo Tech 3. Texas - Histo Tech 4. Kentucky - Histo Tech These are some of my HOTTEST Histology Supevisory positions: 1. Maine - Histology Supervisor 2. New York - Histology Supervisor 3. Indiana - Histology Supervisor 4. Missouri - Histology Supervisor 5. Virginia - Histology Supervisor 6. Colorado - Histology Supervisor Here are some of my HOTTEST Histo Tech bench positions: 1. Florida - Histotechnologists 2. Virginia - Histo Tech 3. Pennsylvania - Histo Tech 4. Oregon - Histo Tech 5. Texas - Histo Tech 6. Massachusetts - Histo Tech (part time 2 positions) 7. Massachusetts - MOHS Tech 8. New York - Histo Tech 9. Indiana - Lead Histo Tech 10. Virginia - Lead Histo Tech 11. Colorado - Histo Tech 12. Maine - Lead Lab Assistant 13. Ohio - Histo Tech If you are interested in these jobs, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well. I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who do not have an email address, if you could pass my query & name on to them I'd be very grateful. I don't want to be a bother - I was told that you were a hands-on Histo Tech or a Lab Supervisor. If you are no longer working in a lab please send me an email and I will remove you from my list of people to contact. However, if you are interested in any of the jobs above, please call me. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: Pam Barker Senior Lab Recruiter Ategra Systems Inc Specialists in Permanent & Contract Staffing 7085 University Blvd. Winter Park, FL 32792 VOICE: 407-671-5800 ext 234 TOLLFREE: 800-466-9919 ext 234 EMAIL: pam@ategra.com To Learn More About Ategra: http://www.ategra.com --------------------------------------------------------------------------------------------- If you do not wish to be contacted, please hit the reply button and let me know. I will then delete your name from my prospect list. --------------------------------------------------------------------------------------------- From gcallis <@t> montana.edu Mon Feb 16 10:17:28 2004 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Storage of Primary Antibodies! In-Reply-To: Message-ID: <3.0.6.32.20040216091728.00befd08@gemini.msu.montana.edu> I generally store antibodies at -27C, never colder, probably more due to ready freezer space. My immunologist who develops monoclonals/polyclonals also stores his in house antibodies at this temperature, but NEVER is a self defrosting freezer. If the antibody is an inhouse supernate, we add 1 ul 1M sodium azide per ml of supernate. Aliquots are made of supernates, and in general we don't bother to purify or concentrate these, they are totally clean. We tend to use these as a supernate, often never diluted further. We have also had supernates with azide added stay potent for YEARS at 4C temps, as long as they are stored in sterile tubes, and aliquoted under sterile conditions in a biohood. The amount of azide is not terribly significant with HRP since so many reagents and rinses take care of getting rid of it before HRP step. We are careful to use good tight screw top tubes, have had problems with flip tops - evaporation was a pain. I think that some antibodies are fussy about storage, per this discussion. At 09:41 AM 2/16/2004 -0500, you wrote: > Hi Pam, > > Thank you for the timely reply. This particular antibody >is an "in-house" antibody so I cannot readily give out the clone >information. My reasoning for aliquoting and freezing was my concern that >there are no preservatives (like sodium azide) added. However, I conducted >one titration experiment with beautiful results. Then it was about 2 1/2 >to 3 weeks later until I could conduct the complete experiment with all >time points included. At this time I started having problems with the >primary antibody dilutions. I suspect that the antibody does not like >being frozen. I am using the same procedure and all the same reagents. > >Jennifer Hoover >BI Research >Eli Lilly and Company > > > > >Pamela Marcum >02/16/2004 09:22 AM > > > To: Jennifer Hoover > cc: > Subject: RE: [Histonet] Storage of Primary Antibodies! > > >Hi Jennifer, > >Some antibodies are fine for storage at -80 and some are not. I think >this >will depend on the antibody. It may be useful to let us now which >antibodies are not doing well as other people may have had experience with >them and have suggestions for you. > >Thanks, > >Pam Marcum? > > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer >> Hoover >> Sent: Monday, February 16, 2004 8:48 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Storage of Primary Antibodies! >> >> >> Histonet, >> >> I have a question regarding the storage (short or longer >> term) of primary antibodies. Is it conducive to aliquot and store >primary >> antibodies at -80C?? Is there the potential for diminished activity >after >> storing the antibody for several weeks to several months? The aliquots >> that were used were thawed and used for one day only. Any volume that >was >> not used was disgarded so freeze/thawing is not the problem. I would >> welcome any insight. Thank you very much! >> >> Jennifer Hoover >> BI Research >> Eli Lilly and Company >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From bill501 <@t> mindspring.com Mon Feb 16 10:33:34 2004 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Storage of Primary Antibodies! In-Reply-To: References: Message-ID: At 8:48 AM -0500 2/16/04, Jennifer Hoover wrote: > I have a question regarding the storage (short or longer >term) of primary antibodies. IN the good days before monoclonals and kits we made GFAP the old fashion wat by injecting rabits, harvesting antibodies and absorbing until we got good specific staining. About a litre of good antibody was aliquoted and stored in a Revco (I think -70?) and it remained good for at least 8 years. If I had a Revco now, I would do this will all antibodies and validate them per case by using controls. -- _______________ Bill Blank, MD Heartland Lab, Inc From Terry.Marshall <@t> rothgen.nhs.uk Mon Feb 16 10:49:21 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Storage of Primary Antibodies! Message-ID: A Revco being? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: 16 February 2004 16:34 To: Jennifer Hoover; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Storage of Primary Antibodies! At 8:48 AM -0500 2/16/04, Jennifer Hoover wrote: > I have a question regarding the storage (short or longer >term) of primary antibodies. IN the good days before monoclonals and kits we made GFAP the old fashion wat by injecting rabits, harvesting antibodies and absorbing until we got good specific staining. About a litre of good antibody was aliquoted and stored in a Revco (I think -70?) and it remained good for at least 8 years. If I had a Revco now, I would do this will all antibodies and validate them per case by using controls. -- _______________ Bill Blank, MD Heartland Lab, Inc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgin <@t> pen.eiu.edu Mon Feb 16 11:20:33 2004 From: cgin <@t> pen.eiu.edu (ikenwosu) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Thank You Message-ID: <4030fbe1.362.4e3d.12959@pen.eiu.edu> hell all!! I just want to thank everybody who helped me out last month by offering leads to getting a good used cryostat. I appreciate the warm and sincere replies that I got. From SohrabB <@t> wmmcpo.ah.org Mon Feb 16 12:08:59 2004 From: SohrabB <@t> wmmcpo.ah.org (Behnaz Sohrab) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Carpet Message-ID: I am looking for a carpet in histlogy room to trap paraffins from going out . Any suggestion ? Currently we are using 3m which is very expensive to replace ! (budget crisis). thanks From dobbin <@t> upei.ca Mon Feb 16 08:10:02 2004 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] apoptosis control tissue In-Reply-To: <4030CFD5.7060904@UNI-BIELEFELD.DE> Message-ID: <4030A50A.8156.573467@localhost> Hi Daniel, Teste is a good choice. Sperm cells are constantly being produced and "apoptosed". Greg Date sent: Mon, 16 Feb 2004 15:12:37 +0100 From: DANIEL EBERHARD To: histonet@pathology.swmed.edu Copies to: Subject: [Histonet] apoptosis control tissue > Dear Histo-Listers, > I would like to check some transgenic mouse tissues for apoptotic > activities. > Thus I?m looking for nice control tissues in which apoptosis is frequent - > e.g . I thought of intestinal epithelium (cypts and villi). > Can you recommend me other tissues/what you do use? > Any suggestions would be helpful, > Thanks in advance > Daniel. > > -- > (-)-(-) > --------------------------- \"/ --- > Dr. Daniel Eberhard =V= > > Developmental Biology > & Molecular Pathology > > Graduate Programe on Pattern Formation > > University of Bielefeld > D 33501 Bielefeld/Germany > > FAX: xx49(0)521-106-5654 (-)-(-) > --------------------------- \"/ --- > =V= > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Greg Dobbin Pathology Lab Atlantic Veterinary College, U.P.E.I. 550 University Ave. Charlottetown, P.E.I. Canada, C1A 4P3 Phone: (902)566-0744 Fax: (902)566-0851 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Making a living is getting; making a life is giving. From Jackie.O'Connor <@t> abbott.com Mon Feb 16 08:29:54 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] apoptosis control tissue Message-ID: I use murine small intestine routinely for an apoptosis control - I have used liver from treated animals in which apoptosis has been induced, but endogenous biotin is sometimes too strong. Overall, I prefer the GI. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics DANIEL EBERHARD Sent by: histonet-bounces@lists.utsouthwestern.edu 02/16/2004 08:12 AM To: histonet@pathology.swmed.edu cc: Subject: [Histonet] apoptosis control tissue Dear Histo-Listers, I would like to check some transgenic mouse tissues for apoptotic activities. Thus I?m looking for nice control tissues in which apoptosis is frequent - e.g . I thought of intestinal epithelium (cypts and villi). Can you recommend me other tissues/what you do use? Any suggestions would be helpful, Thanks in advance Daniel. -- (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V= Developmental Biology & Molecular Pathology Graduate Programe on Pattern Formation University of Bielefeld D 33501 Bielefeld/Germany FAX: xx49(0)521-106-5654 (-)-(-) --------------------------- \"/ --- =V= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Feb 15 16:14:07 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:34 2005 Subject: [Histonet] Cutting 20-50 micron sections Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E12B@simba.kids> Annette, Try warming the block up, in fact don't bother cooling the block at all. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org] Sent: Friday, 13 February 2004 10:14 PM To: Histonet (E-mail) Subject: [Histonet] Cutting 20-50 micron sections Any suggestions for cutting thick (20-50) micron paraffin sections. They curl when they hit the blade and though I am perservering, I was hoping for a tip or two, or three or four or five ................. Annette FeatherstoneHT/MLT Kaleida Health CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From hborgeri <@t> wfubmc.edu Mon Feb 16 12:06:44 2004 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Osteoclasts isolated from blood Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8EA39@EXCHVS2.medctr.ad.wfubmc.edu> Hello everyone, I received the following e-mail request from a researcher in India. However, not being familiar with the type of work she is doing, I thought I would post her questions to the Histonet to see if anyone can help her out. "I am working on expression of salmon calictonin in E.coli and then assaying its action, for which i will be isolating osteoclasts from pheripheral blood by using Histopaque 1077, and M-Csf, etc.Then, i would stain them with TRAP stain,as guided by you, for osteoclasts isolating confirmation. Later, I would be staining with touilidine blue, for analysing the decrease in resorption pits with calcitonin action(both number and radius). I need to enquire: 1.) whether i can do TRAP with osteoclasts isolated from blood. 2.)Incubation period required for TRAP assay and analysing resorption pits. 3.)Is it possible to keep osteoclasts in MEM with Recombinant M_CSF, ODF, FBS,etc for 8-10 days ,during the study, with out using any cell line." Any input would be greatly appreciated. Hermina Hermina M. Borgerink, BA, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax (336) 716-1515 e-mail hborgeri@wfubmc.edu From settembr <@t> umdnj.edu Mon Feb 16 12:15:42 2004 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Revco Message-ID: A Revco is a brand of deep freezers , usually around -80 degrees celcius. Dana Settembre University Hospital, UMNDJ Newark, NJ >>> "Marshall Terry Dr, Consultant Histopathologist" 2/16/2004 8:49:21 AM >>> A Revco being? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: 16 February 2004 16:34 To: Jennifer Hoover; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Storage of Primary Antibodies! At 8:48 AM -0500 2/16/04, Jennifer Hoover wrote: > I have a question regarding the storage (short or longer >term) of primary antibodies. IN the good days before monoclonals and kits we made GFAP the old fashion wat by injecting rabits, harvesting antibodies and absorbing until we got good specific staining. About a litre of good antibody was aliquoted and stored in a Revco (I think -70?) and it remained good for at least 8 years. If I had a Revco now, I would do this will all antibodies and validate them per case by using controls. -- _______________ Bill Blank, MD Heartland Lab, Inc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Mon Feb 16 12:20:19 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Loss of Nuclear Detail in Bone Marrow Message-ID: Nuclear meltdown artefact, you may find text referenced under that title. Maybe it is shortened fixation, maybe it is the presence of organic solvents before the fixation is complete, maybe it is organics contaminating the formalin starting point of processing. Thermo I believe have/or did have advice to the effect of doing a water rinse after the flush cycle to remove traces of eg. xylene/citrosol from the chamber. Best wishes Dave -----Original Message----- From: Bernadette Weston [mailto:bernaweston@hotmail.com] Sent: 16 February 2004 13:26 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Loss of Nuclear Detail in Bone Marrow Our bone marrow latey are losing their nuclear detail, both in the core and aspirate. They are processed in 2 different processors as 2 different fixatives are used. Histochoice for the aspirate and B+ for the core. Our B+ cores have been coming our beautifully but the last few have developed solid blue nuclei with no chromatin detail. The aspirate goes immediately into Histochoice, the core is in B+ for 2 and half hours, in Decal Stat until they float, 15 minutes to an hour. The program is a 13 overnight process. Bernadette Weston HT Barberton Citizens Hospital Barberton, OH _________________________________________________________________ Choose now from 4 levels of MSN Hotmail Extra Storage - no more account overload! http://click.atdmt.com/AVE/go/onm00200362ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ritta69 <@t> iwon.com Mon Feb 16 13:04:55 2004 From: ritta69 <@t> iwon.com (ritta69@iwon.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] fluorojade Message-ID: <20040216190455.B1EDF27C64@email.iwon.com> Has anyone out there done fluorojade B? I have a background problem. I tried increasing time in potassium permanganate and I guess I need to decrease the concentraion of fluorojade. Is it ok to use gelatin coated slides? Any advice would be appreciated. Thanks, Brad _______________________________________________ From dobbin <@t> upei.ca Mon Feb 16 13:43:51 2004 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Images of IHC artefacts Message-ID: <4030F349.12204.188E63A@localhost> Hello Everyone, If any of you have digital images right there on your hard drive (that is to say don't go to any trouble), of some of the more common artefacts encountered when doing IHC staining (eg's positive staining of rbs's due to inadequate quenching, endogenous biotin). A "before and after" or "right and wrong" set for comparison would be extremely helpful. I am going to Mexico this week to offer a wet lab workshop and (at the last minute!) I have been snookered into doing a presentation at the same time. It will be very informal I understand, but a presentation of any kind with out images will soon put everyone to sleep I'm sure! I don't have time to get the images myself before I go, so I am hoping some of you can come through for me. (incidentally, I am not being paid for this workshop-just travel expenses). Thanks in advance if you can help. Greg ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Greg Dobbin Pathology Lab Atlantic Veterinary College, U.P.E.I. 550 University Ave. Charlottetown, P.E.I. Canada, C1A 4P3 Phone: (902)566-0744 Fax: (902)566-0851 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Making a living is getting; making a life is giving. From nperson211 <@t> comcast.net Mon Feb 16 15:12:19 2004 From: nperson211 <@t> comcast.net (nperson211@comcast.net) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] VEGF in rat xenografts Message-ID: <021620042112.22689.18b0@comcast.net> Histonetters, Does anyone do VEGF IHC on FFPE sections of rats that have been implanted with human tumor cell lines? If anyone does and would be willing to share their experiences, I would really appreciate it. Thanks in advance (and with great hope!), Nancy Lemke Hermelin Brain Tumor Center Henry Ford Hospital Detroit, MI From JMyers1 <@t> aol.com Mon Feb 16 15:26:34 2004 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] RE: helicobactor pylori (antibody) Message-ID: Annette (and fellow Histonetters): Biocare Medical offers a prediluted polyclonal Ab to H. pylori that's prediluted especially for Ventana stainers. According to the latest catalog, its $152.00 for a 50-test (5ml) vial, or $278.00 for two (2) 5 ml vials. I hope this info is helpful... J.D. Myers From JWEEMS <@t> sjha.org Mon Feb 16 16:10:28 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] GMS Stain Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD6363@exch2.sjha.org> I've had a few questions regarding the perm solution. I knew I had read something somewhere - p445 Sheehan! She says to remove silver stains from your hands with perm solution, so I thought it should work on tissue as well. It has been quite a while ago. I believe I used it full strengh and kept water close by to swish and dip until it looked just right. j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner, Janet Sent: Tuesday, February 10, 2004 11:16 AM To: 'Joyce Cline'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Stain To remove excess silver ,we dip the slides in 4% Chromic acid and rinse in 1% sodium bisulfite, then check under the scope. If too light, start at the silver step again. Remember that generally a solution is first in the process because it affects the remaining steps, either to enhance or remove! Janet -----Original Message----- From: Joyce Cline [mailto:jcline@wchsys.org] Sent: Thursday, February 05, 2004 3:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] GMS Stain I use a coplin jar of distilled water and I add two -three drops of clorox and then dip the slides until the excess silver comes off. Rinse well in water. ----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PM Subject: [Histonet] GMS Stain > I know there is something to use when the slides get left in the silver > solution to long but have forgotten. Can someone refresh my memory? > Thanks > > > > > ********* Email Confidentiality Statement ********* > Visit http://www.saintfrancis.com/emailconf.asp > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From bills <@t> icpmr.wsahs.nsw.gov.au Mon Feb 16 16:33:52 2004 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] GMS Stain In-Reply-To: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD6363@wsahs.nsw.gov.au> Message-ID: <001201c3f4dc$f4cfd6f0$3187080a@wsahs.nsw.gov.au> Perming solution contains thyoglycolate, if you purchase this in pure form you then dilute it to about 1% and use it the same way as perming solution. Just remember to dilute it in the fume cupboard as the smell is atrocious. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Tuesday, 17 February 2004 9:10 AM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Stain I've had a few questions regarding the perm solution. I knew I had read something somewhere - p445 Sheehan! She says to remove silver stains from your hands with perm solution, so I thought it should work on tissue as well. It has been quite a while ago. I believe I used it full strengh and kept water close by to swish and dip until it looked just right. j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner, Janet Sent: Tuesday, February 10, 2004 11:16 AM To: 'Joyce Cline'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Stain To remove excess silver ,we dip the slides in 4% Chromic acid and rinse in 1% sodium bisulfite, then check under the scope. If too light, start at the silver step again. Remember that generally a solution is first in the process because it affects the remaining steps, either to enhance or remove! Janet -----Original Message----- From: Joyce Cline [mailto:jcline@wchsys.org] Sent: Thursday, February 05, 2004 3:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] GMS Stain I use a coplin jar of distilled water and I add two -three drops of clorox and then dip the slides until the excess silver comes off. Rinse well in water. ----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PM Subject: [Histonet] GMS Stain > I know there is something to use when the slides get left in the silver > solution to long but have forgotten. Can someone refresh my memory? > Thanks > > > > > ********* Email Confidentiality Statement ********* > Visit http://www.saintfrancis.com/emailconf.asp > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From JWEEMS <@t> sjha.org Mon Feb 16 16:39:59 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] GMS Stain Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD6367@exch2.sjha.org> Next time I get a perm I'll have my hair dresser put me in the fume hood... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill Sinai Sent: Monday, February 16, 2004 5:34 PM To: histonet (E-mail) Subject: RE: [Histonet] GMS Stain Perming solution contains thyoglycolate, if you purchase this in pure form you then dilute it to about 1% and use it the same way as perming solution. Just remember to dilute it in the fume cupboard as the smell is atrocious. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Tuesday, 17 February 2004 9:10 AM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Stain I've had a few questions regarding the perm solution. I knew I had read something somewhere - p445 Sheehan! She says to remove silver stains from your hands with perm solution, so I thought it should work on tissue as well. It has been quite a while ago. I believe I used it full strengh and kept water close by to swish and dip until it looked just right. j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner, Janet Sent: Tuesday, February 10, 2004 11:16 AM To: 'Joyce Cline'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Stain To remove excess silver ,we dip the slides in 4% Chromic acid and rinse in 1% sodium bisulfite, then check under the scope. If too light, start at the silver step again. Remember that generally a solution is first in the process because it affects the remaining steps, either to enhance or remove! Janet -----Original Message----- From: Joyce Cline [mailto:jcline@wchsys.org] Sent: Thursday, February 05, 2004 3:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] GMS Stain I use a coplin jar of distilled water and I add two -three drops of clorox and then dip the slides until the excess silver comes off. Rinse well in water. ----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PM Subject: [Histonet] GMS Stain > I know there is something to use when the slides get left in the silver > solution to long but have forgotten. Can someone refresh my memory? > Thanks > > > > > ********* Email Confidentiality Statement ********* > Visit http://www.saintfrancis.com/emailconf.asp > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From pruegg <@t> colobio.com Mon Feb 16 17:24:02 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] VEGF in rat xenografts In-Reply-To: <021620042112.22689.18b0@comcast.net> Message-ID: Nancy, I do VEGF on rat tissue all the time, xenographs and human tumor cell implanted. I use monoclonal vegf from Santa Cruz at 1:200 with proteinase k digestion for 5 min. I am using the the same vegf on sheep tissue now with good results. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of nperson211@comcast.net Sent: Monday, February 16, 2004 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VEGF in rat xenografts Histonetters, Does anyone do VEGF IHC on FFPE sections of rats that have been implanted with human tumor cell lines? If anyone does and would be willing to share their experiences, I would really appreciate it. Thanks in advance (and with great hope!), Nancy Lemke Hermelin Brain Tumor Center Henry Ford Hospital Detroit, MI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.browne <@t> auckland.ac.nz Mon Feb 16 17:58:55 2004 From: j.browne <@t> auckland.ac.nz (Jeremy Browne) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] unsubscribe Message-ID: 2nd attempt. Please. Jeremy Browne Liggins Institute University of Auckland NZ (09) 373 7599 extn 86444 j.browne@auckland.ac.nz From Rcartun <@t> harthosp.org Mon Feb 16 17:25:16 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] NPHS1 antibody Message-ID: Is anyone aware of an antibody to the "Congenital nephrotic syndrome (NPHS1)" gene product? Thank you. Richard Cartun From weber.326 <@t> osu.edu Mon Feb 16 22:30:57 2004 From: weber.326 <@t> osu.edu (Nancy E Weber) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Artefact in Technovit embedded sections Message-ID: <017201c3f50e$d6b3a2f0$64996ba4@JOHNSONLAB> Hello to fellow histonetters, I am processing some canine jaw samples and taking sections of teeth and the surrounding bone. I am using the Exakt system and embedding in the Technovit 7200. I am grinding down to a range around 70 - to 100 microns. Under the microscope I'm seeing dark areas that look like the plastic may be cracking all thru the sections. Could it be that I'm just not getting good infiltration of the Technovit during processing? Any insight would be appreciated. Thanks Nancy. Nancy Weber Research Associate Veterinary Clinical Sciences Ohio State University From ekaplan <@t> squ.edu.om Tue Feb 17 01:54:11 2004 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] buying control materials Message-ID: Good morning, Can anyone give me information on suppliers who sell control slides for histochemistry? Thanks in advance, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman From nyilmaz <@t> mersin.edu.tr Tue Feb 17 04:15:12 2004 From: nyilmaz <@t> mersin.edu.tr (=?windows-1254?Q?Nejat_Y=FDlmaz?=) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] normal serum preparation Message-ID: <000801c3f53e$ee3d5440$4f01a8c0@mersin.edu.tr> Dear Histonetters... Does anybody know how can I prepare the normal serum from fresh animal blood for immunohistochemistry? Thanks in advance... Dr. Necat Y?lmaz From docmichel <@t> netbulmail.com Tue Feb 17 05:24:22 2004 From: docmichel <@t> netbulmail.com (izzet oguz) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] (no subject) Message-ID: <2477.193.255.128.130.1077017062.webmail@mail1.netbulmail.com> Is there any problem about subscription? From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Feb 17 07:27:05 2004 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Carpet Message-ID: <3D502BBF5356D31184650090275B750D0346C6F9@mail.cooley-dickinson.org> Contact Mats and Matting Company (Division of CMS,Inc.) 1-800-872-9700 -----Original Message----- From: Behnaz Sohrab [mailto:SohrabB@wmmcpo.ah.org] Sent: Monday, February 16, 2004 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Carpet I am looking for a carpet in histlogy room to trap paraffins from going out . Any suggestion ? Currently we are using 3m which is very expensive to replace ! (budget crisis). thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From rmarcos <@t> icbas.up.pt Tue Feb 17 07:33:45 2004 From: rmarcos <@t> icbas.up.pt (ricardo marcos) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Cutting 20-50 micron sections In-Reply-To: <1CF2E2E5BB36D5119E7A0008C791F3740800E12B@simba.kids> Message-ID: <000001c3f55a$b0898490$3505a8c0@porto.icbas.up.pt> I agree...I've worked with 30 micron paraffin liver sections...you should try to cut with a very slow velocity, using a non disposable microtome blade (I've used a plane wedge type) and warm the block, by breathing on, as you cut every section. Hope this helps. Ricardo Marcos Lab Histology and Embryology ICBAS - Univ Porto, Portugal -----Mensagem original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Em nome de Tony Henwood Enviada: domingo, 15 de Fevereiro de 2004 22:14 Para: 'Featherstone, Annette'; Histonet (E-mail) Assunto: RE: [Histonet] Cutting 20-50 micron sections Annette, Try warming the block up, in fact don't bother cooling the block at all. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org] Sent: Friday, 13 February 2004 10:14 PM To: Histonet (E-mail) Subject: [Histonet] Cutting 20-50 micron sections Any suggestions for cutting thick (20-50) micron paraffin sections. They curl when they hit the blade and though I am perservering, I was hoping for a tip or two, or three or four or five ................. Annette FeatherstoneHT/MLT Kaleida Health CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lfidgen <@t> vt.edu Tue Feb 17 08:34:11 2004 From: lfidgen <@t> vt.edu (Laura Fidgen) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] artery sectioning Message-ID: <6.0.0.22.0.20040217093225.025e7808@pop.vt.edu> I am having a difficult time sectioning artery for the HT practical. I have increased the temperature of my water bath but the folds still persist. Any suggestions? Laura L. Fidgen, MScF, BSc Laboratory and Research Practioner VMRCVM, Laboratory Central Receiving Blacksburg, VA 24060-0443 From HOOVER_JENNIFER <@t> LILLY.COM Tue Feb 17 08:49:20 2004 From: HOOVER_JENNIFER <@t> LILLY.COM (Jennifer Hoover) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Cartilage Marker for IHC? Message-ID: Histonet, I am looking for suggestions for an IHC marker that would be constitutively expressed in the cartilage of rat knee joints. Can anyone recommend a clone and the Company to purchase the antibody from. Thank you very much! Jennifer Hoover Bone and Inflammation Research Eli Lilly and Company From HOOVER_JENNIFER <@t> LILLY.COM Tue Feb 17 08:58:31 2004 From: HOOVER_JENNIFER <@t> LILLY.COM (Jennifer Hoover) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Cartilage Marker for IHC? Message-ID: From AInman <@t> stmarygj.com Tue Feb 17 08:58:24 2004 From: AInman <@t> stmarygj.com (Anna Inman) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Rapid Tissue Processors / Automated Embedding Message-ID: We are in the early stages of looking at Tissue-Tek's Rapid Tissue Processor and Automated Embedding System. Does anyone have any knowledge / experience with these they would be willing to share? If anyone has any thoughts on the Slide and Block Labelers from Tissue-Tek that would also be great. Thank you in advance. Anna Inman Dept of Pathology St Mary's Hospital Grand Junction, CO (970) 244-7624 fax (970)244-2100 ainman@stmarygj.com This electronic message and all contents contain information from St. Mary's Hospital which may be attorney-client privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender immediately and destroy the original message and all copies. Thank you. From AInman <@t> stmarygj.com Tue Feb 17 09:14:12 2004 From: AInman <@t> stmarygj.com (Anna Inman) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Dotting pens Message-ID: The dotting pens our Pathologists and Cytotechs like are being discontinued by Pilot (Extra Fine Point Permanent Marker). We have tried the Ultra Fine PinPoint Pen from Eberhard Faber but without success. Does anyone have any recommendations? Thank you. Anna Inman SMH Pathology (970)244-7624 fax (970) 244-2100 ainman@stmarygj.com This electronic message and all contents contain information from St. Mary's Hospital which may be attorney-client privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender immediately and destroy the original message and all copies. Thank you. From spoulos <@t> saa.ars.usda.gov Tue Feb 17 09:20:50 2004 From: spoulos <@t> saa.ars.usda.gov (Sylvia Poulos) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Dotting pens Message-ID: I got the colony counter from scienceware (F37862-0000) if you actually need to keep a running count of your dots. More expensive than a marker but it prevents cramped thumbs from the bus driver clickers we had before. Sylvia Sylvia P. Poulos USDA-ARS-Animal Physiology Research Unit Athens, GA 30605 706-583-8279 706-542-0399 (fax) From JLE <@t> rice.willmar.mn.us Tue Feb 17 09:20:32 2004 From: JLE <@t> rice.willmar.mn.us (Jennifer Englin) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] nexes stainer Message-ID: We also have the Nexxus and are having problems with the pH (7.0 or greater) of the special stain wash it should be 6.0. Has anyone else had this problem?? If so how can we solve it? Jennifer Englin HT(ASCP), PA Rice Memorial Hospital Willmar, MN >>> "Joyce Cline" 02/16/04 09:18AM >>> I use the Ventana, we perform several different stains at a time, but there is a problem of not being able to run silver stains along with others because you run out of space for the stain vials. I find that even though we sometimes run the Ventana 2 - 3 times a day, it saves by not having all these stains done by hand, which frees my techs to perform other areas of the job. The quality is excellant, sometimes you have to tweek the stain to satisfy your paths. ----- Original Message ----- From: "Gudrun Lang" To: "Histonetliste" Sent: Saturday, February 14, 2004 4:34 AM Subject: [Histonet] nexes stainer A question to those, who work with the nexes special stainer: We are going to test the nexes. We have max. 20 slides to stain per day. For example 8 PAS, 3 CAB (trichrom), 3 NASD, 3 BB, 2 Retic., 3 Jones-Methenamin and so on. And some of the stains can't be performed on the nexes. Doing it by hand we need most of the time about 1 to 1,5 hours in the morning. Can you give me an advice, what amount of stains per day makes sense to use an automatic stainer like the nexes? And how long do you need from preparing the slides until the ready stains.? Thanks in advance Gudrun Lang, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GAshton <@t> PICR.man.ac.uk Tue Feb 17 09:17:19 2004 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] H&E stainers Message-ID: Dear all, Following on from my message last week about the pro's and con's of tissue processors available in the UK, does anybody have any tips / advice or experience of H&E slide stainers. I'm more interested in the models like the Shandon Gemini model, not the conventional linistain. Many thanks in advance. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From jcline <@t> wchsys.org Tue Feb 17 09:42:49 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Behnaz Sohrab / carpet in Histology Message-ID: <004801c3f56c$b31867a0$1d2a14ac@wchsys.org> I use disposable stickey mats in front of each door to catch the paraffin on the bottom of the shoes. I peal each layer off when it gets coated with paraffin or dirt. (each mat contains about 30 sheets) I get these from a cleaning supply company. From sladd <@t> hsc.usf.edu Tue Feb 17 09:39:22 2004 From: sladd <@t> hsc.usf.edu (Sharron Ladd) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] artery sectioning In-Reply-To: <6.0.0.22.0.20040217093225.025e7808@pop.vt.edu> References: <6.0.0.22.0.20040217093225.025e7808@pop.vt.edu> Message-ID: <403235AA.6030100@hsc.usf.edu> Patience...patience....patience.... Rather than increasing water bath temperature I decreased it. I left my artery sections (5 micron not 4) on a low temp. (about 37-42 degrees C) water bath for up to an hour....then dried the slides vertically at room temp. overnight. I probably cut 50 or more slides before I found the perfect section (of course then I had to get the perfect stain). People had told me to put a cap full of methanol in my water bath but I didn't have any success with that. Another trick was to put Downy fabric softener in the water bath but I didn't know how much and it just made a big mess of everything. Good luck. Sharron P.S. I recently did the HT and the HTL and passed them both. Feel free to email me if you need any more help. Laura Fidgen wrote: > I am having a difficult time sectioning artery for the HT practical. > I have increased the temperature of my water bath but the folds still > persist. Any suggestions? > > > Laura L. Fidgen, MScF, BSc > Laboratory and Research Practioner > VMRCVM, Laboratory Central Receiving > Blacksburg, VA 24060-0443 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From davenhv <@t> hotmail.com Tue Feb 17 10:19:17 2004 From: davenhv <@t> hotmail.com (Dave McClister) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] double immunofluorescent staining Message-ID: I'm in search of a protocol for double staining. I'm currrently testing: First Primary= CD-4 First Secondary= FITC labeled Second Primary= MMP-8 Second Secondary= Rhodamine Any help would be greatly appreciated. Dave McClister 843-789-6826 wk 843-792-0590 pg#1-1274 davenhv@hotmail.com _________________________________________________________________ Stay informed on Election 2004 and the race to Super Tuesday. http://special.msn.com/msn/election2004.armx From Linke_Noelle <@t> Allergan.com Tue Feb 17 10:31:23 2004 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] collagen antibodies Message-ID: Does anyone have a working protocol/supplier for collagen antibodies on formalin-fixed paraffin embedded monkey tissue? Thank you!!! Noelle Linke, BS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From DDittus787 <@t> aol.com Tue Feb 17 11:36:09 2004 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] buying control materials Message-ID: <1C20FCD8.61939519.0A1F969F@aol.com> zymed sells very nice IHC control slides for some of the more difficult to find controls, contact them 1-800-874-4494 dana From dmnelson <@t> iastate.edu Tue Feb 17 11:59:01 2004 From: dmnelson <@t> iastate.edu (Diane Gerjets) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Re: H & E Prep Cost In-Reply-To: References: Message-ID: <6.0.1.1.2.20040217114823.01ae1988@dmnelson.mail.iastate.edu> >Today's Topics: > > 1. H&E Prep Only Cost?? (Survey) (Wayne Holland) > 2. RE: H&E Prep Only Cost?? (Survey) (Mass Histology Service) > 3. Re: nexes stainer (Gudrun Lang) > 4. Varistain wells? (a-lisowski@northwestern.edu) > 5. Varistain wells? (a-lisowski@northwestern.edu) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Sat, 14 Feb 2004 14:25:22 -0500 >From: "Wayne Holland" >Subject: [Histonet] H&E Prep Only Cost?? (Survey) >To: Histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; format=flowed > >I have all the figures for the actual cost of getting the job done. >But can you all, the laboratories that do povide such a service, tell me >what the current charge is for an H&E prep? What I mean is from specimen >bottle to finished slide, "period". This does not include any >interpretation. Your input would be extremely helpful, once again thanks >much. >Wayne, We charge $9.00 per slide. That includes grossing, processing, embedding, cutting, staining and coverslipping. I hope this is helpful. Diane Gerjets Iowa State University College of Veterinary Medicine Ames, Iowa 50011 >essage: 4 >Date: Sun, 15 Feb 2004 8:27:30 -0600 >From: a-lisowski@northwestern.edu >Subject: [Histonet] Varistain wells? >To: histonet@lists.utsouthwestern.edu >Message-ID: <200402151427.i1FERXpt006794@hecky.it.northwestern.edu> >Content-Type: text/plain > > >Hi! >Does anyone have a varistain staining well willing to give away a sell? >Thanks >Andrew Lisowski >a-lisowski@northwestern.edu > > > > > >------------------------------ > >Message: 5 >Date: Sun, 15 Feb 2004 8:27:35 -0600 >From: a-lisowski@northwestern.edu >Subject: [Histonet] Varistain wells? >To: histonet@lists.utsouthwestern.edu >Message-ID: <200402151427.i1FERhWi006986@hecky.it.northwestern.edu> >Content-Type: text/plain > > >Hi! >Does anyone have a varistain staining well willing to give away a sell? >Thanks >Andrew Lisowski >a-lisowski@northwestern.edu > > > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 3, Issue 19 >*************************************** From dmnelson <@t> iastate.edu Tue Feb 17 12:18:38 2004 From: dmnelson <@t> iastate.edu (Diane Gerjets) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Fite's Acid-Fast Stain for Nocardia Message-ID: <6.0.1.1.2.20040217121136.01b4b628@dmnelson.mail.iastate.edu> Hi, We use the fite's stain for nocardia. We are having trouble with blotchy background staining due to the xylene/peanut oil used in the stain. We blot our slides and rinse them with warm water, but still have the background blotchy staining. Thank-you for any help that you could give me. Diane Gerjets Iowa State University College of Veterinary Medicine Ames, Iowa 50011 From rfail <@t> toolkitmail.com Tue Feb 17 12:29:15 2004 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Fite's Acid-Fast Stain for Nocardia Message-ID: <40325d7b.2e1.7735.1458989308@toolkitmail.com> Hell Diane, We do the Fite's routinely with a little modification. After xylene/peanut oil, we put the slides in xylene for 10 minutes, then wash in running water. NO BLOTTING. The stain comes out as clean as the Kinyoun's procedure for both Nocardia and Leprosy with no loss of staining. Rena Fail Hi, > We use the fite's stain for nocardia. We are having > trouble with blotchy background staining due to the > xylene/peanut oil used in the stain. We blot our slides > and rinse them with warm water, but still have the > background blotchy staining. Thank-you for any help that > you could give me. > > Diane > Gerjets > > Iowa State > University > > College of > Veterinary Medicine > > Ames, > Iowa 50011 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Tue Feb 17 12:51:27 2004 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Xylene Substitue / Formula 83 Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BDE7@EXCHANGE1.huntingtonhospital.com> Has anyone tried the new xylene substitute from CBG Biotech called Formula 83? They claim it's faster and safer than xylene, it dries faster than xylene, dissolves paraffin faster than xylene, etc. etc. Laurie Colbert Huntington Hospital From AFeatherstone <@t> KaleidaHealth.Org Tue Feb 17 12:46:50 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Beta 2 Microglobulin Message-ID: Is any kidney good for this control(Beta 2 Microglobulin) or does it have to be a dialysis kidney? Thanks in advance! Annette FeatherstoneHT/MLT CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Feb 17 13:05:54 2004 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Xylene Substitue / Formula 83 Message-ID: <3D502BBF5356D31184650090275B750D0346C6FA@mail.cooley-dickinson.org> We just received our free sample. I'll be happy to tell you of our progress as we test it. Stacy -----Original Message----- From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Sent: Tuesday, February 17, 2004 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitue / Formula 83 Has anyone tried the new xylene substitute from CBG Biotech called Formula 83? They claim it's faster and safer than xylene, it dries faster than xylene, dissolves paraffin faster than xylene, etc. etc. Laurie Colbert Huntington Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From rfail <@t> toolkitmail.com Tue Feb 17 13:44:04 2004 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Re: TYPO is my face red!!!! Should of course be Hello Message-ID: <40326f04.1bf.308f.953840733@toolkitmail.com> I apologize to the list and to Diane Rena Fail Hell Diane, > We do the Fite's routinely with a little modification. > After xylene/peanut oil, we put the slides in xylene for > 10 minutes, then wash in running water. NO BLOTTING. The > stain comes out as clean as the Kinyoun's procedure for > both Nocardia and Leprosy with no loss of staining. > Rena Fail > Hi, > > We use the fite's stain for nocardia. We are having > > trouble with blotchy background staining due to the > > xylene/peanut oil used in the stain. We blot our slides > > and rinse them with warm water, but still have the > > background blotchy staining. Thank-you for any help that > > you could give me. > > > > > > Diane Gerjets > > > > Iowa > > State University > > > > College of > > Veterinary Medicine > > > > Ames, > > Iowa 50011 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Feb 17 14:04:04 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Re: TYPO is my face red!!!! Should of course be He llo Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD637D@exch2.sjha.org> But its so funny! Thanks for the laugh. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of rfail Sent: Tuesday, February 17, 2004 2:44 PM To: rfail@TOOLKITMAIL.COM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: TYPO is my face red!!!! Should of course be Hello I apologize to the list and to Diane Rena Fail Hell Diane, > We do the Fite's routinely with a little modification. > After xylene/peanut oil, we put the slides in xylene for > 10 minutes, then wash in running water. NO BLOTTING. The > stain comes out as clean as the Kinyoun's procedure for > both Nocardia and Leprosy with no loss of staining. > Rena Fail > Hi, > > We use the fite's stain for nocardia. We are having > > trouble with blotchy background staining due to the > > xylene/peanut oil used in the stain. We blot our slides > > and rinse them with warm water, but still have the > > background blotchy staining. Thank-you for any help that > > you could give me. > > > > > > Diane Gerjets > > > > Iowa > > State University > > > > College of > > Veterinary Medicine > > > > Ames, > > Iowa 50011 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From Dave.Pizi <@t> carolinashealthcare.org Tue Feb 17 14:44:12 2004 From: Dave.Pizi <@t> carolinashealthcare.org (Pizi, Dave) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Heidenhain Hematoxylin for Amoeba Message-ID: <7E22BEDC45D51449B847E15983E99BAD721D69@dcr-xchg-04.Carolinas.org> Hey We have been looking for this procedure without any luck. We have the solutions but not the protocol (?). If anyone can share one I would appreciate it. Thanks Dave Pizi Charlotte ----------------------------------------- This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From haldana <@t> unimoron.edu.ar Tue Feb 17 15:36:21 2004 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Artery section (Laura Fidgen) Message-ID: <001e01c3f59e$1850eb20$7504a8c0@um.edu> Dear Laura I work with sections of the aorta. First of all I cut 5?m, then I use a tipicall water bath with hot water and when the sections lies over the slide I press the section with my finger (puting a humedified (with the wather of the bath) filter paper betwen the section and the finger) then dried the slides vertically at room or hot temeprature. My english is not good i hope that you understand me Thanks Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html From yangpw <@t> umich.edu Tue Feb 17 16:15:41 2004 From: yangpw <@t> umich.edu (yangpw@umich.edu) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] few questions about IHC DAB staining Message-ID: <1077056141.4032928d6b996@mail.umich.edu> HI, All, I have been doing c-Fos IHC stainging and using Vector ABC kit and DAB as chromagen. THe protocol I use worked fine in the past, but recently I have confronted couple problems. ANy suggestion would be greatly appreciated!! 1) The staining is kind of light. I have incubated tissues (rat brain, 30 um, cryostat-sliced, 4%PFA perfused and 30% sucrose cryopretected before slicing, free floating) in DAB color reaction solu for one and a half hours, and the cell staining is ok under microscope but still light comparing to the intensity I used to get. Background is ok. In the past I used same protocol, same concentration of antibody, and only need eight minutes to reach pretty decent intensity. 2) The sections tend to stick to each other during the incubation of primary antibody (at 4c for 48 hours), which cause uneven staining. The overlaped tissue tends to be light stained. I use specific staining plate, which has mesh under the wells to facilitate the tissue transfering during washings. Is it possible related to the staining plate, or to shaking speed? How can I avoid that? 3) Recently I am going to try calbindin (D-28k) staining on rat brain to delineate the core and shell of nucleus accumbens. I have searched some papers and it seems many people use monoclonal mouse anti-calbindin (Sigma). But I am kind of concerned about the nonspecific crossreactivity between secondary antibody and rat tissue, since rat and mouse are close. Some people use polyclonal rabbit anti calbindin, but it will crossreact with calrentinin, which might compromise the clear delineation between core and shell. Any opinion is very welcome. Thanks in advance. Pengwei Yang Biopsychology Program Psychology Depart. University of Michigan From mcauliff <@t> umdnj.edu Tue Feb 17 19:09:41 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] staining of anterior pituitary? In-Reply-To: <4030F349.12204.188E63A@localhost> References: <4030F349.12204.188E63A@localhost> Message-ID: <4032BB55.3000308@umdnj.edu> Deal Histonetters: I need the experience and opinions of those of you who do clinical work. What sort of staining is used for anterior pituitary glands these days? Immuno looking for specific cell types or is there still some PAS+Orange G, Azan, Herlant's, etc. staining going on? Thanks! Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From AnthonyH <@t> chw.edu.au Tue Feb 17 16:57:44 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] normal serum preparation Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E136@simba.kids> Nejat, Collect the blood in a sterile 10ml plain centrifuge tube. Allow to clot (5-10 minutes should do it) Centrifuge 10min 1500rpm in a standard centrifuge Collect supernatant (the serum) Remember do not add anti-coagulant (eg EDTA, Citrate or Heparin). When you centrifuge the blood, you will have plasma. Ask for advice from your Haematology Department. Regards Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Nejat Yilmaz [mailto:nyilmaz@mersin.edu.tr] Sent: Tuesday, 17 February 2004 9:15 PM To: histonet Subject: [Histonet] normal serum preparation Dear Histonetters... Does anybody know how can I prepare the normal serum from fresh animal blood for immunohistochemistry? Thanks in advance... Dr. Necat Y?lmaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From c.m.vanderloos <@t> amc.uva.nl Wed Feb 18 03:25:16 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] RE: double immunofluorescent staining Message-ID: <18c875184fcb.184fcb18c875@amc.uva.nl> Dear Dave, Given that your first and second primary are both mouse antibodies you may try the following: MMP-8, Ms anti-Ms/Rhodamine Normal Mouse serum (1:10, 15 min, do not wash) CD4/FITC (it's not difficult to purchase this one) Lots of success with your staining! Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- Date Tue, 17 Feb 2004 16:19:17 +0000 >From "Dave McClister" To histonet@lists.utsouthwestern.edu Subject [Histonet] double immunofluorescent staining I'm in search of a protocol for double staining. I'm currrently testing: First Primary= CD-4 First Secondary= FITC labeled Second Primary= MMP-8 Second Secondary= Rhodamine Any help would be greatly appreciated. Dave McClister 843-789-6826 wk 843-792-0590 pg#1-1274 davenhv@hotmail.com From antje.marcantonio <@t> pharma.novartis.com Wed Feb 18 03:28:52 2004 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] vWf in rat kidneys - the answer Message-ID: Hello Histonetters vWf staining in kidneys is not a mystery anymore ! Adapting my protocol to the procedure I found in the report mentioned below , I got wonderful strong staining even in the glomeruli ! Pepsin was the trick ...(Proteinase K didn't help) So now we run two completely different protocols one for kidneys and one for lungs. 1: Kidney Int. 2000 Dec;58(6):2599-610. The vasculopathy of autosomal dominant polycystic kidney disease: insights from animal models. Arnaout MA. Best regards, Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland From c.m.vanderloos <@t> amc.uva.nl Wed Feb 18 03:31:45 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] RE: VEGF in rat xenografts Message-ID: <215470214dc7.214dc7215470@amc.uva.nl> Dear Nancy, We have been using VEGF from Santa Cruz (A-20, sc-152) rabbit antibody successfully on human FFPE tissues (HIER with EDTA9.0, 1:200 overnight 4C, EnVision+ detection). A mamma tumor serves as a good positive control. The data sheet said there is a cross-reaction with other species including rat. Hope this helps. Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From nperson211@comcast.net Date Mon, 16 Feb 2004 21:12:19 +0000 To histonet@lists.utsouthwestern.edu Subject [Histonet] VEGF in rat xenografts Histonetters, Does anyone do VEGF IHC on FFPE sections of rats that have been implanted with human tumor cell lines? If anyone does and would be willing to share their experiences, I would really appreciate it. Thanks in advance (and with great hope!), Nancy Lemke Hermelin Brain Tumor Center Henry Ford Hospital Detroit, MI From v.lamanna <@t> abdn.ac.uk Wed Feb 18 04:11:12 2004 From: v.lamanna <@t> abdn.ac.uk (Vincenzo La Manna) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Collagen IV & Integrin alpha6 Message-ID: <4544.139.133.196.188.1077099072.squirrel@www.abdn.ac.uk> Hi Histonetters, Does anyone know any supllier or have any information about antibodies anti-collagen IV and Integrin alpha6 raised in mouse wiyh a good specificity for bovine tissue? Thanks, -- La Manna Vincenzo Department of Agriculture and Forestry, University of Aberdeen, Hilton Place block M AB24 4FA, Aberdeen , UK. Telephone; 01224 274259 Fax; 01224 273731 e-mail v.lamanna@abdn.ac.uk From Lizbeth_Kelly <@t> hgsi.com Wed Feb 18 07:20:43 2004 From: Lizbeth_Kelly <@t> hgsi.com (Lizbeth_Kelly@hgsi.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] IHC markers Message-ID: Does anyone have a source for anti C3A or C5a complement proteins, NK cells(CD56), or macrophage and monocyte markers that will cross react with Guinea Pig on either frozen or paraffin sections? From twheelock <@t> mclean.harvard.edu Wed Feb 18 08:01:03 2004 From: twheelock <@t> mclean.harvard.edu (Timothy R. Wheelock) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] NITRIC ACID SUBSTITUTE? Message-ID: <4033701F.9070003@mclean.harvard.edu> Hi Everyone: I have been using pure Nitric Acid for years to acid-clean glassware for my 20% silver nitrate Bielschowsky stain. I would like to find a safer substitute for it. Any ideas? What do other people use to acid-clean their glassware? Thanks for any advice you can give. Tim Wheelock Brain Bank McLean Hospital Belmont MA Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From DOOLEEO <@t> shands.ufl.edu Wed Feb 18 09:35:33 2004 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] folate receptor antibody Message-ID: Dear Histonet, Does anyone know of a folate receptor antibody that works well in formalin fixed paraffin embedded tissue? Vendor? Pretreatments? Thanks in advance Elaine Dooley Shands Hospital Gainesville FL 352-265-0111 ext 7-2114 From Hedley.Glencross <@t> CMMC.nhs.uk Wed Feb 18 10:38:54 2004 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] dotting pens Message-ID: Hi Anna We use Pilot "ulta fine super colour markers" here in Manchester. They are available from Pilot UK, as we've only recently bought some. 4 colours, black, blue, green & red. These are very similar to those given away by Shandon, Cytyc and SurePath. I'm not sure if these are the same as the ones you say are discontinued in the US. I hope this helps Hedley Glencross Manchester Cytology Centre UK Date: Tue, 17 Feb 2004 08:14:12 -0700 From: Anna Inman Subject: [Histonet] Dotting pens To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain The dotting pens our Pathologists and Cytotechs like are being discontinued by Pilot (Extra Fine Point Permanent Marker). We have tried the Ultra Fine PinPoint Pen from Eberhard Faber but without success. Does anyone have any recommendations? Thank you. Anna Inman SMH Pathology (970)244-7624 fax (970) 244-2100 ainman@stmarygj.com From dmnelson <@t> iastate.edu Wed Feb 18 12:56:37 2004 From: dmnelson <@t> iastate.edu (Diane Gerjets) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Thank-you for solution to fite's stain Message-ID: <6.0.1.1.2.20040218125052.01b31f08@dmnelson.mail.iastate.edu> Rena, Thank-you for the solution to the blotchy background on the fite's stain. The straight xylene after the peanut oil/xylene solution was the trick! Thanks again!! Diane Gerjets Iowa Sate University College of Veterinary Medicine From maweber <@t> scripps.edu Wed Feb 18 13:19:51 2004 From: maweber <@t> scripps.edu (Martin Weber) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] alkaline phosphatase Message-ID: <4033BAD7.1050101@scripps.edu> Hello, I might need to do a dual staing procedure on paraformaldehyde fixed fresh frozen sections. One marker will be stained with peroxidase (DAB). As a second marker for IHC I read about alkaline phosphatase (FastRed) which gives rise to a red color in addition to the brown color of the peroxidase staining. To me this combination seems to be a little bit unfortunate because the colors are sort of overlapping. Does anybody know of an alternative and complimentary method for dual staining which might give rise to a different color other than red ? Unfortunately due to special circumstances Immunofluorecence is not an option. Any help is much appreciated. Thanks a lot ! Martin Weber The Scripps Research Institute Dept. of Molecular and Experimental Medicine 10550 North Torey Pines Road La Jolla, 92037 Phone: 858-784-8612 Fax: 858-784-2030 E-Mail: maweber@scripps.edu -------------- next part -------------- Martin Weber M.D. Work address: The Scripps Research Institute Department of Molecular and Experimental Medicine 10550 North Torrey Pines Road, Mail drop MEM 175 La Jolla, CA. 92037, U.S.A. Phone at work: 001-858-784-8612 Fax at work: 001-858-784-2030 E-Mail: maweber@scripps.edu Home address: 7265 Charmant Drive, Apt 628 San Diego, CA., 92122, U.S.A. Phone at home: 001-858-452-0544 From mbryhan <@t> NORTHERNHEALTH.ORG Wed Feb 18 13:25:12 2004 From: mbryhan <@t> NORTHERNHEALTH.ORG (Mary Bryhan) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] CLIA Message-ID: Hi, Can anyone point me in the direction of the section of the Federal Register where it discusses the level of testing complexity for routine Histology? Mary From kspencer <@t> utmem.edu Wed Feb 18 13:53:31 2004 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] alkaline phosphatase In-Reply-To: <4033BAD7.1050101@scripps.edu> Message-ID: <20E0A111-624C-11D8-99DB-000393967904@utmem.edu> Yes, use plain DAB for your first AB and DAB nickel sulfate for your second. The procedure takes 4 days because you do them one at a time (with overnight incubation) You get brown for one and blue black for the other. Anyway, that is what we do. Kathleen Spencer, HT Lab Manager UTHSC On Wednesday, February 18, 2004, at 01:19 PM, Martin Weber wrote: > Hello, > I might need to do a dual staing procedure on paraformaldehyde fixed > fresh frozen sections. One marker will be stained with peroxidase > (DAB). As a second marker for IHC I read about alkaline phosphatase > (FastRed) which gives rise to a red color in addition to the brown > color of the peroxidase staining. To me this combination seems to be a > little bit unfortunate because the colors are sort of overlapping. > Does anybody know of an alternative and complimentary method for dual > staining which might give rise to a different color other than red ? > Unfortunately due to special circumstances Immunofluorecence is not an > option. > Any help is much appreciated. Thanks a lot ! > > Martin Weber > > The Scripps Research Institute > Dept. of Molecular and Experimental Medicine > 10550 North Torey Pines Road > La Jolla, 92037 > Phone: 858-784-8612 > Fax: 858-784-2030 > E-Mail: maweber@scripps.edu > Martin Weber M.D. > > Work address: > The Scripps Research Institute > Department of Molecular and Experimental Medicine > 10550 North Torrey Pines Road, Mail drop MEM 175 > La Jolla, CA. 92037, U.S.A. > Phone at work: 001-858-784-8612 > Fax at work: 001-858-784-2030 > E-Mail: maweber@scripps.edu > > > Home address: > 7265 Charmant Drive, Apt 628 > San Diego, CA., 92122, U.S.A. > Phone at home: 001-858-452-0544 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Wed Feb 18 14:15:01 2004 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] alkaline phosphatase In-Reply-To: <20E0A111-624C-11D8-99DB-000393967904@utmem.edu> Message-ID: <000601c3f65b$e47b79a0$3601a8c0@brownpathology.net> Martin, There are other alk phos chromogens available such as NBT/BCIP which yields a blue-black color product, and Vector has several chromogens for HRP, including a brick red, a purple, and it seems like some others. I personally like using the brick red HRP and the blue-black AP chromogens. If you want to use 2 HRP protocols you can do as Kathleen suggested. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Spencer Sent: Wednesday, February 18, 2004 1:54 PM To: Martin Weber Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] alkaline phosphatase Yes, use plain DAB for your first AB and DAB nickel sulfate for your second. The procedure takes 4 days because you do them one at a time (with overnight incubation) You get brown for one and blue black for the other. Anyway, that is what we do. Kathleen Spencer, HT Lab Manager UTHSC On Wednesday, February 18, 2004, at 01:19 PM, Martin Weber wrote: > Hello, > I might need to do a dual staing procedure on paraformaldehyde fixed > fresh frozen sections. One marker will be stained with peroxidase > (DAB). As a second marker for IHC I read about alkaline phosphatase > (FastRed) which gives rise to a red color in addition to the brown > color of the peroxidase staining. To me this combination seems to be a > little bit unfortunate because the colors are sort of overlapping. > Does anybody know of an alternative and complimentary method for dual > staining which might give rise to a different color other than red ? > Unfortunately due to special circumstances Immunofluorecence is not an > option. > Any help is much appreciated. Thanks a lot ! > > Martin Weber > > The Scripps Research Institute > Dept. of Molecular and Experimental Medicine > 10550 North Torey Pines Road > La Jolla, 92037 > Phone: 858-784-8612 > Fax: 858-784-2030 > E-Mail: maweber@scripps.edu > Martin Weber M.D. > > Work address: > The Scripps Research Institute > Department of Molecular and Experimental Medicine > 10550 North Torrey Pines Road, Mail drop MEM 175 > La Jolla, CA. 92037, U.S.A. > Phone at work: 001-858-784-8612 > Fax at work: 001-858-784-2030 > E-Mail: maweber@scripps.edu > > > Home address: > 7265 Charmant Drive, Apt 628 > San Diego, CA., 92122, U.S.A. > Phone at home: 001-858-452-0544 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Fauck <@t> ccdhb.org.nz Wed Feb 18 14:26:09 2004 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] RE: Histonet Digest, Vol 3, Issue 22 Message-ID: <9952FA35C61B4F4F90DC0B2D58FE68A960E5AE@WN0NTEML01.hiq.net.nz> Hi Anna, I have search for years for a suitable replacement marking pen for our cytology filters that we use for our cyto preparations of all non-gynae cases and marking of our smears etc We use with great satifaction now Super-Fine Pentel SF70 USA Hope that you can locate ( try otherwise their website ) them in the USA Cheers, Robert Fauck Histo/Cyto Lab Wellington Hospital New Zealand Message: 2 Date: Tue, 17 Feb 2004 08:14:12 -0700 From: Anna Inman Subject: [Histonet] Dotting pens To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain The dotting pens our Pathologists and Cytotechs like are being discontinued by Pilot (Extra Fine Point Permanent Marker). We have tried the Ultra Fine PinPoint Pen from Eberhard Faber but without success. Does anyone have any recommendations? Thank you. Anna Inman SMH Pathology (970)244-7624 fax (970) 244-2100 ainman@stmarygj.com C&C DHB Secure Mail Server. ******************************************************************************** This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital and Coast District Health Board. (AC_S001) No Viruses were detected in this message. ******************************************************************************** From djohnson14 <@t> hotmail.com Wed Feb 18 14:32:11 2004 From: djohnson14 <@t> hotmail.com (Dave Johnson) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Marking pen Message-ID: Pacific SW Labs carries an excellent marking pen. I have been told this pen lasts up to at least 3 times longer than some of the other pens on the market and doesnt have the tendency to dry up, etc. Their 800# is 866-429-0618 _________________________________________________________________ Find and compare great deals on Broadband access at the MSN High-Speed Marketplace. http://click.atdmt.com/AVE/go/onm00200360ave/direct/01/ From brett_connolly <@t> merck.com Wed Feb 18 14:23:44 2004 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] alkaline phosphatase Message-ID: Martin, Another possibility comes to mind. You might consider changing the peroxidase substrate. I have used TrueBlue Peroxidase Substrate from Kirkegaard & Perry Laboratories, it's more sensitive than DAB and if you play with the conditions you can end up with a turquoise reaction product. http://www.kpl.com/webpromotions/index.cfm?pID=60 Regards, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin Weber Sent: Wednesday, February 18, 2004 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alkaline phosphatase Hello, I might need to do a dual staing procedure on paraformaldehyde fixed fresh frozen sections. One marker will be stained with peroxidase (DAB). As a second marker for IHC I read about alkaline phosphatase (FastRed) which gives rise to a red color in addition to the brown color of the peroxidase staining. To me this combination seems to be a little bit unfortunate because the colors are sort of overlapping. Does anybody know of an alternative and complimentary method for dual staining which might give rise to a different color other than red ? Unfortunately due to special circumstances Immunofluorecence is not an option. Any help is much appreciated. Thanks a lot ! Martin Weber The Scripps Research Institute Dept. of Molecular and Experimental Medicine 10550 North Torey Pines Road La Jolla, 92037 Phone: 858-784-8612 Fax: 858-784-2030 E-Mail: maweber@scripps.edu ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From joseph-galbraith <@t> uiowa.edu Wed Feb 18 15:13:11 2004 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] NITRIC ACID SUBSTITUTE? Message-ID: <5D03ED7B9391D4119D9B0008C76B7B24030085F0@uihc-mail1.uihc.uiowa.edu> Tim: We rinse with Contrad 70 after a thorough washing with alconox in a dishwasher running very high quality distilled/deionized H2O. This Contrad 70 (it is a liquid) rinse takes place just before using the glassware. We pour the Contrad 70 in and out of its alloquat container using the glassware to be cleaned. I like to let a film of Contrad set on the glass surface for 30 seconds after pouring back and forth, then you must completely rinse off any residual Contrad 70 with DI before use. The Contrad 70 can be reused (from alloquat) many times but discard it if it darkens or gets contaminated with any dyes. A key element in this approach is that the glassware must be well cleaned with Alconox and high quality water before proceding to the Contrad 70. We have not used any strong acids to clean glassware for many years since switching to this method and we perform numerous silver based stains including Biel's. Good luck, Joe Galbraith -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Timothy R. Wheelock Sent: Wednesday, February 18, 2004 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NITRIC ACID SUBSTITUTE? Hi Everyone: I have been using pure Nitric Acid for years to acid-clean glassware for my 20% silver nitrate Bielschowsky stain. I would like to find a safer substitute for it. Any ideas? What do other people use to acid-clean their glassware? Thanks for any advice you can give. Tim Wheelock Brain Bank McLean Hospital Belmont MA Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From hfedor <@t> jhmi.edu Wed Feb 18 15:35:21 2004 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Marking pen Message-ID: Do you know if that is this a xylene free marker? thanks, Helen Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. >>> Dave Johnson 02/18/04 03:32PM >>> Pacific SW Labs carries an excellent marking pen. I have been told this pen lasts up to at least 3 times longer than some of the other pens on the market and doesnt have the tendency to dry up, etc. Their 800# is 866-429-0618 _________________________________________________________________ Find and compare great deals on Broadband access at the MSN High-Speed Marketplace. http://click.atdmt.com/AVE/go/onm00200360ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DeBari <@t> mail.holyname.org Wed Feb 18 15:37:19 2004 From: DeBari <@t> mail.holyname.org (DeBari@mail.holyname.org) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] 88305 BILLING In-Reply-To: Message-ID: When the cytotechnologist goes on a cat scan guided biopsy, she makes 2 touch preps from the biopsy. the cytology touch preps are evaluated for adequacy and the biopsy is evaluated the next day. In this case, the patient is charged 88161 for the cytology specimen and 88305 for the surgical biopsy specimen. Billing is telling us that 88161 needs a 59 modifier. Is this how other labs are billing this? Any help is greatly appreciated. Christina Holy Name Hospital Cytology From amarusk1 <@t> FAIRVIEW.ORG Wed Feb 18 16:21:44 2004 From: amarusk1 <@t> FAIRVIEW.ORG (ANN MARUSKA) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] caspase-3 Message-ID: Hi histonetters, I have a researcher who will be doing a study using caspase-3 as an IHC stain. The specimens will be colected in Turkey and processed here in the US. Do you forsee any problems if the specimens are collected and fixed in 10% NBF and then transferred to 70% alcohol and shipped here? They would probably be in alcohol about 2-3 weeks. Thanks for your help! Ann Ann Maruska Fairview-University Medical Center Mpls. MN 55454 amarusk1@fairview.org 612-273-9119 The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:ANN MARUSKA EMAIL;WORK;PREF;NGW:AMARUSK1@FAIRVIEW.ORG ORG:;LAB N:MARUSKA;ANN TEL;WORK:612-273-9119 END:VCARD From j.browne <@t> auckland.ac.nz Wed Feb 18 16:38:33 2004 From: j.browne <@t> auckland.ac.nz (Jeremy Browne) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] please unsubscribe (3rd attempt) Message-ID: Please!! From yangpw <@t> umich.edu Wed Feb 18 17:59:50 2004 From: yangpw <@t> umich.edu (yangpw@umich.edu) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Thanks! (about few questions about IHC DAB staining) Message-ID: <1077148790.4033fc76941f5@mail.umich.edu> HI, All, Thanks so much for the warm replies I got. They are very helpful. About the light staining with DAB, I have some more details I would like to supplement. 1)The DAB I used is from Sigma (DAB tablet D5905, 10 mg/tablet, store at -20 c). I made fresh DAB solution about one hour before I used it (10 mg DAB, 10ul 30% H2O2 in 30ml TBS, I add DAB first and stir until resolve and filter it using 0.2 um filter, add H2O2 right before using it). The interesting thing is after 1.5 hours incubation in DAB solu, the background did not enhanced much and actually was relatively low. I will try ordering a new batch of DAB and H2O2. At the same time, do I need to increse the concentration of DAB or make other changes to my DAB protocol? There are also some commercial liquid DAB available, are they reliable after long-term storage? 2) The stains I got are doubtless c-Fos staining and I store aliquoted primary antibody in the -20 c freezer. So there is no repeat thaw/freeze problem and the same batch of primary antoibody was good in previous staining. THe antibody came in with 200 ul and i just simply divided it into several 10 uls and stored at -20c. I notice some labs use glycerol to dilute the antibogy before put in the freezer. Does that help to sustain the reactivity of antibody? 3) The Vector ABC kit I used is still before expiring date. The concentration of ABC reagent I used though was 1:1,000 of both A and B reagent instead of what the company recommend. And I have tried this kind of concentration many times before and it worked fine. But I am not sure if I need to increase the concentraion since the ABC kit I used has been in refrig for several months. Any more suggestion is appreciated. Thanks. Pengwei Yang Biopsychology Program Psychology Depart. University of Michigan From bicresource <@t> hotmail.com Wed Feb 18 18:13:46 2004 From: bicresource <@t> hotmail.com (J Bueno) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] TEST Message-ID: TEST _________________________________________________________________ Find and compare great deals on Broadband access at the MSN High-Speed Marketplace. http://click.atdmt.com/AVE/go/onm00200360ave/direct/01/ From bicresource <@t> hotmail.com Wed Feb 18 18:25:15 2004 From: bicresource <@t> hotmail.com (J Bueno) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Available Message-ID: Available Leitz 1400 Slege Microtome in excellent condition. Looking for best offer. Mike MacDougall (800) 783-9424 Ext.114 _________________________________________________________________ Find and compare great deals on Broadband access at the MSN High-Speed Marketplace. http://click.atdmt.com/AVE/go/onm00200360ave/direct/01/ From KAELPERS <@t> aol.com Wed Feb 18 20:21:31 2004 From: KAELPERS <@t> aol.com (KAELPERS@aol.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Behnaz Sohrab / carpet in Histology Message-ID: <80.5a1f6d0.2d6577ab@aol.com> Yes, we just started using these too at our microtome and embedding stations. They can be purchased at Pacific Southwest in white or blue and different sizes. We use white since paraffin is and so far they are working out nicely. LaDonna From KAELPERS <@t> aol.com Wed Feb 18 20:33:24 2004 From: KAELPERS <@t> aol.com (KAELPERS@aol.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Retic Stain Kit Message-ID: <147.22771267.2d657a74@aol.com> I used the Polyscientific's retic stain for a registry slide a while ago. I thought the stain was nice,(boring but nice), one of the few I had problems with. The pathologist reviewed the slide for me too with no poor feedback. Are you not getting good differentiation? Are you using a counterstain? What are the problems? LaDonna From rocan <@t> mac.com Thu Feb 19 00:46:23 2004 From: rocan <@t> mac.com (rocan@mac.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] NITRIC ACID SUBSTITUTE? In-Reply-To: <4033701F.9070003@mclean.harvard.edu> References: <4033701F.9070003@mclean.harvard.edu> Message-ID: <550E1EE0-62A7-11D8-B830-000A9589219E@mac.com> Try RBS-35 Detergent Concentrate from Pierce at http://www.piercenet.com. I have been using it for years with great results. Their claim is that this detergent is as good as chromic acid mixture. M. Rocio Sierra-Honigmann, MD, Ph.D. Lab Director Engineered Wound Repair Laboratory Division of Plastic Surgery Cedars Sinai Research Institute Los Angeles, CA On Feb 18, 2004, at 6:01 AM, Timothy R. Wheelock wrote: > Hi Everyone: > > I have been using pure Nitric Acid for years to acid-clean glassware > for my 20% silver nitrate Bielschowsky stain. > I would like to find a safer substitute for it. > Any ideas? > What do other people use to acid-clean their glassware? > Thanks for any advice you can give. > > Tim Wheelock > Brain Bank > McLean Hospital > Belmont MA > > > > Any information, including protected health information (PHI), > transmitted > in this email is intended only for the person or entity to which it is > addressed and may contain information that is privileged, confidential > and or > exempt from disclosure under applicable Federal or State law. Any > review, > retransmission, dissemination or other use of or taking of any action > in > reliance upon, protected health information (PHI) by persons or > entities other > than the intended recipient is prohibited. If you received this email > in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From antje.marcantonio <@t> pharma.novartis.com Thu Feb 19 00:53:51 2004 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] vWf in rat kidneys - correct reference Message-ID: Hello, yesterday I sent you first the wrong reference and the mail with the correct one obviously didn't come through. Please find here the right reference. Sorry again for the mistake. Neutralizing TGF-1 antibody infusion in neonatal rat delays in vivo glomerular capillary formation Ailian Liu, Alan Dardik, and Barbara J. Ballermann Kidney International Volume 56 Issue 4 Page 1334 - October 1999 http://www.blackwell-synergy.com/links/doi/10.1046/j.1523-1755.1999.00661.x/pdf Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland Tel. +41 61 324 6730 From i_stain <@t> yahoo.com Thu Feb 19 02:11:40 2004 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] CD Markers Message-ID: <20040219081140.781.qmail@web42001.mail.yahoo.com> Hi, Should try Zymed's polyclonal CD3, with pepsin digestion on FFPE. The Ab is highly sensitive & specific. Scott -----Original Message----- From: Linresearch@aol.com [mailto:Linresearch@aol.com] Sent: Friday, February 06, 2004 5:00 PM To: histonet@pathology.swmed.edu Subject: [Histonet] CD Markers Hi, I would appreciate any hints on working-up the following Abs on FFPE rodent tissues: Cd3 Cd45R Cd21 Thanks in advance. lin __________________________________ Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. http://antispam.yahoo.com/tools From i_stain <@t> yahoo.com Thu Feb 19 02:17:25 2004 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Re: VEGF in rat xenografts Message-ID: <20040219081725.84631.qmail@web42004.mail.yahoo.com> Hi Nancy, Should polyclonal VEGF (18-0254) from Zymed on FFPE with 15 min HIER, citrate pH 6.0 Scott CSU Message: 10 Date: Mon, 16 Feb 2004 21:12:19 +0000 From: nperson211@comcast.net Subject: [Histonet] VEGF in rat xenografts To: histonet@lists.utsouthwestern.edu Message-ID: <021620042112.22689.18b0@comcast.net> Histonetters, Does anyone do VEGF IHC on FFPE sections of rats that have been implanted with human tumor cell lines? If anyone does and would be willing to share their experiences, I would really appreciate it. Thanks in advance (and with great hope!), Nancy Lemke Hermelin Brain Tumor Center Henry Ford Hospital Detroit, MI __________________________________ Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. http://antispam.yahoo.com/tools From i_stain <@t> yahoo.com Thu Feb 19 02:27:20 2004 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Re: DAKO synaptophysin on Ventana ES Message-ID: <20040219082720.67824.qmail@web42002.mail.yahoo.com> Hi, We use ready-to-use polyclonal Synaptophysin (08-4130) from Zymed stained pancreas on the Ventana with Protease I 8 min, 32 min protocol with wonderful staining. Scott SCU Message: 18 Date: Thu, 29 Jan 2004 20:06:24 -0500 From: "david moses" Subject: [Histonet] DAKO synaptophysin on Ventana ES To: Message-ID: <002d01c3e6cd$48fa6740$d4f63ad0@turbo> Content-Type: text/plain; charset="iso-8859-1" Is anyone using DAKO's synaptophysin monoclonal (sy38) on their Ventana ES or NexES? I used citrate buffer in a decloaker at a 1:20 titer, with no staining at all in a pancreas control. Renee Boston-Moses HT Virtua Health Ststems Voorhees,New Jersey __________________________________ Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. http://antispam.yahoo.com/tools From ttroyer <@t> pcllab.com Thu Feb 19 05:49:03 2004 From: ttroyer <@t> pcllab.com (Travis Troyer) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] toluidine blue Message-ID: <002d01c3f6de$5f0c2570$6601010a@Peterson.local> I was wondering if anyone had any tips for controls and procedures for the toluidine blue stain for mast cells. I am not seeing any differentiation in my stain. Thanks. From Jeannette.Mitchell <@t> vtmednet.org Thu Feb 19 07:33:46 2004 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Thanks! (about few questions about IHC DAB staining) Message-ID: Does anyone out there do the EGFR test? I know that Dakocytomation is coming out with it but someone here is requesting and wants it now. Jeannette Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From Delventh3 <@t> aol.com Thu Feb 19 07:47:13 2004 From: Delventh3 <@t> aol.com (Delventh3@aol.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Substitute for acid cleaning Message-ID: <139.2b499f04.2d661861@aol.com> Hello to all - I found that cleaning glass with 10% chlorox for at least 30 minutes and follow with several changes of distilled deionized water will clean glassware for silver staining. When I have time I will soak overnight, rinse well, dry, and then cover with parafilm untill time to use. Hopefully it will work for you. I have used this method in several different labs throughout the country (I do temp histology) and it has work well each time. On an aside, I thought the typo was funny --made my day. It's like those times when the bad words come out unexpectedly under stress and you can't take them back!!!!! Have a good day everyone. We are lucky to be in such an interesting, varied field. Priscilla - Still in Central Wyoming From Ronnie_Houston <@t> bshsi.com Thu Feb 19 07:57:38 2004 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Thanks! (about few questions about IHC DAB stainin g) Message-ID: <530361BF03351B4CAE5270A05D3037B5036730A2@bsrexms01.BSHSIR.COM> Erbitux is the monoclonal antibody therapy that has been FDA approved for treatment of colon cancers. It specifically binds to EGFR, and effectively blocks some kinases resulting in inhibiting cell growth, inducing apoptosis, and decreasing MMP and VEGF production. EGFR is expressed in many normal epithelia. It is over-expressed in many human cancers including colon and rectal. The clinical studies to date, and those that gained FDA approval of the drug, have required positive expression of EGFR for enrollment. The results were based on the use of DakoCytomation's EGFR pharmDx kit. One concern of oncologists at present is that the clinical trails never included any patients who had negative expression of EGFR, so they do not know if these patients will respond to the drug also. Dako's kit has been about for some time now. Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 (804) 287 7906 - fax ronnie_houston@bshsi.com -----Original Message----- From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org] Sent: Thursday, February 19, 2004 8:34 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thanks! (about few questions about IHC DAB staining) Does anyone out there do the EGFR test? I know that Dakocytomation is coming out with it but someone here is requesting and wants it now. Jeannette Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From RFail <@t> Charleston.net Thu Feb 19 08:06:23 2004 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Thanks! (about few questions about IHC DAB staining) In-Reply-To: Message-ID: <000001c3f6f1$8fa87900$9311a6a5@rena> Yes, Jeanette We purchase it from Zymed, dilute 1:150, HIER at pH 6.0, with envision + on the DAKO autostainer Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Jeannette M Sent: Thursday, February 19, 2004 8:34 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thanks! (about few questions about IHC DAB staining) Does anyone out there do the EGFR test? I know that Dakocytomation is coming out with it but someone here is requesting and wants it now. Jeannette Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan <@t> mail.holyname.org Thu Feb 19 08:22:46 2004 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Fw: cornflaking on slides - using sakura tape coverslipper Message-ID: ----- Forwarded by Nirmala Srishan/HNH on 02/19/2004 09:22 AM ----- Nirmala Srishan/HNH To 02/17/2004 10:09 histonet@lists.utsouthwestern.edu AM cc Subject cornflaking on slides - using sakura tape coverslipper Hello, We are currently using a tape coverslipper from Sakura. Although this is not a daily occurrence it might affect 10-15% of the slides on certain days. We have tried to follow all the remedy given by Sakura and still having problems. Has anyone else confronted this issue? If so, how was the issue resolved? Thank you Nirmala Srishan From djohnson14 <@t> hotmail.com Thu Feb 19 08:32:43 2004 From: djohnson14 <@t> hotmail.com (Dave Johnson) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Fw: cornflaking on slides - using sakura tapecoverslipper Message-ID: Have you tried the tape that Mercedes Medical carries. Try calling Chad at 800-331-2716 THanks Dave Johnson Pacific SW Labs www.psl-equip.com >From: srishan@mail.holyname.org >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Fw: cornflaking on slides - using sakura >tapecoverslipper >Date: Thu, 19 Feb 2004 09:22:46 -0500 > > > > > > >----- Forwarded by Nirmala Srishan/HNH on 02/19/2004 09:22 AM ----- > > Nirmala > Srishan/HNH > To > 02/17/2004 10:09 histonet@lists.utsouthwestern.edu > AM cc > > Subject > cornflaking on slides - using > sakura tape coverslipper > > > > > > > > > >Hello, > >We are currently using a tape coverslipper from Sakura. Although this is >not a daily occurrence it might affect 10-15% of the slides on certain >days. We have tried to follow all the remedy given by Sakura and still >having problems. Has anyone else confronted this issue? If so, how was >the issue resolved? > >Thank you >Nirmala Srishan > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get fast, reliable access with MSN 9 Dial-up. Click here for Special Offer! http://click.atdmt.com/AVE/go/onm00200361ave/direct/01/ From bwhitaker <@t> brownpathology.com Thu Feb 19 09:08:16 2004 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Substitute for acid cleaning In-Reply-To: <139.2b499f04.2d661861@aol.com> Message-ID: <001001c3f6fa$338de520$3601a8c0@brownpathology.net> I also also used the chlorox with good results, but I just wanted to caution people to not leave any metal instruments soaking in there overnight, or for very long. I have ruined more forceps than I care to count by dropping them in the dishwater to disinfect them, and forgetting and leaving them there all night :) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Delventh3@aol.com Sent: Thursday, February 19, 2004 7:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Substitute for acid cleaning Hello to all - I found that cleaning glass with 10% chlorox for at least 30 minutes and follow with several changes of distilled deionized water will clean glassware for silver staining. When I have time I will soak overnight, rinse well, dry, and then cover with parafilm untill time to use. Hopefully it will work for you. I have used this method in several different labs throughout the country (I do temp histology) and it has work well each time. On an aside, I thought the typo was funny --made my day. It's like those times when the bad words come out unexpectedly under stress and you can't take them back!!!!! Have a good day everyone. We are lucky to be in such an interesting, varied field. Priscilla - Still in Central Wyoming _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Thu Feb 19 09:07:42 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] A Few Things I learned About Cutting Thick Sections Message-ID: Thanks to everyone for all the tips for cutting 20-50 micron sections!! 1. Warm blocks in water bath for a few seconds. 2. Hold down section with a brush 3. Lay section on room temp water bath first. Use a non treated slide to transfer. 4. Lay section on 42 degree water bath and watch it uncurl!! Annette Featherstone HT/MLT CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From AFeatherstone <@t> KaleidaHealth.Org Thu Feb 19 09:04:19 2004 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] A Message-ID: CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From v.lamanna <@t> abdn.ac.uk Thu Feb 19 09:18:52 2004 From: v.lamanna <@t> abdn.ac.uk (Vincenzo La Manna) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] primers ER alpha & beta, PR, TGFbeta Message-ID: <1617.139.133.196.188.1077203932.squirrel@www.abdn.ac.uk> I'm looking for primers to ER alpha & beta, PR and TGFbeta for RT PCR in bovine tissue. First time I'm gonna try PCR :-( so, I would be very pleased about any suggestion, reference or help from anyone! thanks in advance, -- La Manna Vincenzo Department of Agriculture and Forestry, University of Aberdeen, Hilton Place block M AB24 4FA, Aberdeen , UK. Telephone; 01224 274259 Fax; 01224 273731 e-mail v.lamanna@abdn.ac.uk From JMacDougall <@t> CuraGen.com Thu Feb 19 09:37:09 2004 From: JMacDougall <@t> CuraGen.com (MacDougall, John) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] DAKO CSA II kit - alternate source for reagents Message-ID: <41B4AA6F8065BA49AF3B9B877824EB380145CAC2@ENTERPRISE.CURAGEN.COM> Hi All I'm looking for an alternate source for the so-called "amplification reagent" in the DAKO CSA II kit. This amplification reagent is a fluorescein-tyramide conjugate, that upon catalysis by HRP deposits fluorescein in the tissue section. This can then be detected by fluorescence microscopy or IHC using anti-fluorescein. Thanks for any and all help. Sincerely John R. MacDougall, Ph.D. Project Leader Preclinical Development - Toxicology/Pathology CuraGen Corporation 322 East Main St. Branford, CT, 06405 Voice: 203-871-4320 Fax: 203-481-1102 LEGAL NOTICE: Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From funderwood <@t> mcohio.org Thu Feb 19 09:32:11 2004 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] toluidine blue Message-ID: I use a 0.5% toluidine blue in 0.5NHCl (90ml. DH2O, 4.2ml conc. HCl) for 30 minutes. Rapidly dehydrate in 3 changes of absolute alcohol, xylene, and coverslip. A section of skin works good for control. fred >>> "Travis Troyer" 02/19/04 06:49AM >>> I was wondering if anyone had any tips for controls and procedures for the toluidine blue stain for mast cells. I am not seeing any differentiation in my stain. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victoria.spoon <@t> bassett.org Thu Feb 19 10:24:47 2004 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Autopsy competency assessment/training Message-ID: <1D9402BE940DF64E965196B5162C800108DC68@exchange.bassett.org> Does anyone have a competency assessment or training document for autopsy/morgue responsibilities that you would be willing to share with me? I'm looking for something to train morgue assistants (not PA's). Or if you can offer articles or suggestions of where to look for information, I'd appreciate that as well. I already checked CAP website and didn't really find what I was looking for. Thanks in advance for any help. Vicki Spoon Bassett Hospital Cooperstown, NY From maweber <@t> scripps.edu Thu Feb 19 10:36:02 2004 From: maweber <@t> scripps.edu (Martin Weber) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Ki67 and dual staining Message-ID: <4034E5F2.2030003@scripps.edu> Good morning everybody, I wanted to thank again everybody who responded to my two questions. I got a lot of helpful advice. Thanks a lot ! Martin From rocan <@t> mac.com Thu Feb 19 10:41:48 2004 From: rocan <@t> mac.com (rocan@mac.com) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] NITRIC ACID SUBSTITUTE? Message-ID: <8307AEF2-62FA-11D8-B830-000A9589219E@mac.com> Try RBS-35 Detergent Concentrate from Pierce at http://www.piercenet.com. I have been using it for years with great results. Their claim is that this detergent is as good as chromic acid mixture. M. Rocio Sierra-Honigmann, MD, Ph.D. Lab Director Engineered Wound Repair Laboratory Division of Plastic Surgery Cedars Sinai Research Institute Los Angeles, CA From StarkusL <@t> ummhc.org Thu Feb 19 11:43:31 2004 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Best fixative for Mouse lung Message-ID: For those of you doing rodent lung, what is your preferred fixative and I assume perfusion is your best method? From Rcartun <@t> harthosp.org Thu Feb 19 11:54:10 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Thanks! (about few questions about IHC DAB staining) Message-ID: Yes, we test for EGFr by IHC. Richard Cartun >>> "Mitchell, Jeannette M." 02/19/04 08:33AM >>> Does anyone out there do the EGFR test? I know that Dakocytomation is coming out with it but someone here is requesting and wants it now. Jeannette Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lizchlipala <@t> premierhistology.com Thu Feb 19 11:59:57 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] Best fixative for Mouse lung In-Reply-To: Message-ID: <000001c3f712$33089ba0$74d48a80@LIZ> Laurie We would fix in either 10% NBF or paraformaldehyde. We would inflate the lungs with the fixative and then place the entire lungs in a container with fixative. You can do this with a blunt tipped syringe. Carefully remove the sternum and dissect around the trachea and lungs, you can leave the heart attached. You do not want to knick the lungs or you will not get good results. For rats we would remove the lungs heart and trachea in their entirety, you need to make sure you have a good portion of the trachea present, we would then place the blunt end of the needle into the trachea and tie the needle in place with suture, then we would inflate the lungs with fixative and then remove the needle carefully and tie off the trachea so the solution would remain in the lungs and then place the lungs and heart in fixative. For mice we would do the same thing only that we inflated the lungs while they were in the chest cavity. Hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Starkus, Laurie Sent: Thursday, February 19, 2004 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best fixative for Mouse lung For those of you doing rodent lung, what is your preferred fixative and I assume perfusion is your best method? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timothy_m_coskran <@t> groton.pfizer.com Thu Feb 19 12:24:07 2004 From: timothy_m_coskran <@t> groton.pfizer.com (Coskran, Timothy M) Date: Fri Sep 16 15:22:35 2005 Subject: [Histonet] amplification reagent Message-ID: <75FBD3E83B69D711A9E600080261980C02BC9CA5@groexmb04bak.pfizer.com> John, I don't know of an alternate source for the "amplification reagent" in the CSA II kit, but we've been using Cy5-tyramide from Perkin Elmer with very good results. Tim Coskran Pfizer PGRD Groton, CT LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From t-sherman <@t> comcast.net Thu Feb 19 12:44:13 2004 From: t-sherman <@t> comcast.net (Todd Sherman) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Re: Best fixative for Mouse lung [from Histonet Digest, Vol 3, Issue 25] In-Reply-To: References: Message-ID: <403503FD.6060908@comcast.net> -----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hello Laurie, It's been a while since I've done the fixation but I would cast my vote for 4% paraformaldehyde (fresh) in PBS buffer. Perfusion would be ideal, but if the tissue is small enough, immersion works fine. We usually immersed/fixed the tissue in cool fixative (i.e. refrigerated temperatures) until it was time to process and paraffin infiltrate in an automated processor. At that point, routine automated processing in 10% NBF was the norm (if any buffered formalin steps were used at all). Hope this helps, Todd Todd Sherman President HistoSoft Corporation www.histosoft.com - Home www.myhistosoft.com - Member Services histonet-request@lists.utsouthwestern.edu wrote: | When replying, please edit your Subject line so it is more specific | than "Re: Contents of Histonet digest..." | | | Today's Topics: | | 10. Best fixative for Mouse lung (Starkus, Laurie) | ---------------------------------------------------------------------- Message: 10 Date: Thu, 19 Feb 2004 12:43:31 -0500 From: "Starkus, Laurie" Subject: [Histonet] Best fixative for Mouse lung To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" For those of you doing rodent lung, what is your preferred fixative and I assume perfusion is your best method? - ------------------------------ -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.2.3 (MingW32) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org iD8DBQFANQP7EmHXdrslGRcRArh1AJ4si0JM/AHO2VGih8c5y/6q48tFRgCfWVEQ Da/fYIytUcIlppppUKfVUnI= =C9dd -----END PGP SIGNATURE----- From joeamateur <@t> hotmail.com Thu Feb 19 13:06:19 2004 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] while we're talking about von Willebrand Factor... Message-ID: Another REALLY basic question from the Rookie... We're about to do our first vWf assay today, and none of us in this lab have done Ab staining before. We've got primary Ab and secondary Ab, and I'm reasonably certain I can get it to work once I get past the Ag retrieval step. For that, the only enzyme we have in the lab right now is 0.25% trypsin/0.1mM EDTA mix. I've tried to check the references supplied previously, but all require subscriptions that I don't have access to. Here's my question: is that concentration of trypsin adequate for Ag retrieval, and if so, how long would it incubate for (assume 37 degrees)? (For that matter, would the chelative effects of the added EDTA mess things up?) >From what I gather, proteinase K digestion would be the best way to go, but unfortunately time is of the essence and we can't get it fast enough. So the more important question, I guess, is whether I tell my PI that it can't be done with what we have on hand, or whether it can be done (though not perfectly). Can anyone out there advise? --Many, many thanks, Jack England _________________________________________________________________ Dream of owning a home? Find out how in the First-time Home Buying Guide. http://special.msn.com/home/firsthome.armx From giorgia.setti <@t> kcl.ac.uk Thu Feb 19 12:58:38 2004 From: giorgia.setti <@t> kcl.ac.uk (Giorgia Setti) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] (no subject) Message-ID: Giorgia Setti mailto:giorgia.setti@kcl.ac.uk Dear All, I am desperate because I am looking for an antibody against 5-lipoxygenase to use on rat tissue (immunohistochemistry,frozen section);I can't find it.........Did anybody look for this antibody?? Thank you very much for all your help!! Giorgia From scoop <@t> mail.nih.gov Thu Feb 19 13:37:32 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] bone marrow IHC Message-ID: Hi, thanks to all the people who helped me with perfusions. I have another question related to a technique I haven't done before: immunofluorescence on bone marrow. I'm trying to use F4/80 to stain for macrophages and ter-119 to stain for erythroid lineage cells as well as various other antibodies. Do people have an opinion on what is the best way to fix the tissue, and does anyone have suggestions on the F4/80 and ter-119 (including suggestions of other antibodies to try)? I get a lot of background with these markers (although maybe it's real and not background). Is immunofluorescence the way to go for bone marrow or is immunohistochemistry better? Are smears better or should I be doing paraffin embedded sections? Thanks, I appreciate any and all suggestions! Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From dmnelson <@t> iastate.edu Thu Feb 19 14:27:01 2004 From: dmnelson <@t> iastate.edu (Diane Gerjets) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Toluidine Blue O Message-ID: <6.0.1.1.2.20040219141926.01b78c10@dmnelson.mail.iastate.edu> Travis, This is the procedure that we use in our lab. 1. Deparaffinize and rehydrate to water. 2. Stain in 1% Toluidine Blue O for 1 minute. ( Mix toluidine blue with distilled water ). 3. Tap water rinse. 4. Dehydrate, clear and mount. This has worked fine for us. We just use a mast cell control. Diane Gerjets Iowa Sate University College of Veterinary Medicine Ames, Iowa 50011 From ljb <@t> medicine.wisc.edu Thu Feb 19 15:02:10 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] amplification reagent Message-ID: Dear Histonetters, At an NSA meeting a few years ago I remember hearing that Perkin Elmer owned the patent for this amplification technology. At that time I was also told that only Dako had purchased the rights to sell the system under their name CAT. If I am wrong about this would a PerkinElmer's rep please let us know? LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> "Coskran, Timothy M" 02/19/04 12:24PM >>> John, I don't know of an alternate source for the "amplification reagent" in the CSA II kit, but we've been using Cy5-tyramide from Perkin Elmer with very good results. Tim Coskran Pfizer PGRD Groton, CT LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zandra <@t> gwu.edu Thu Feb 19 15:34:02 2004 From: zandra <@t> gwu.edu (Alexandra Allison de Sousa) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] fragile tissue for immunohistochemisty Message-ID: <40352BCA.8030309@gwu.edu> Hello! I have been doing immunohistochemistry on some very fragile and large sections of ape brain. The procedure requires so many transfers (washing and 2 pretreatments in addition to primary antibody, secondary...) that the tissue is shredded by the time it is stained and I am ready to mount it on a slide. I assume that I could save the tissue some damage by mounting first before -- has anyone tried this? Would it work? Any other ideas? Thanks! Alexandra From AnthonyH <@t> chw.edu.au Thu Feb 19 16:33:31 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] alkaline phosphatase Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E139@simba.kids> Try using cobalt chloride to change the colour of the DAB Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Martin Weber [mailto:maweber@scripps.edu] Sent: Thursday, 19 February 2004 6:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alkaline phosphatase Hello, I might need to do a dual staing procedure on paraformaldehyde fixed fresh frozen sections. One marker will be stained with peroxidase (DAB). As a second marker for IHC I read about alkaline phosphatase (FastRed) which gives rise to a red color in addition to the brown color of the peroxidase staining. To me this combination seems to be a little bit unfortunate because the colors are sort of overlapping. Does anybody know of an alternative and complimentary method for dual staining which might give rise to a different color other than red ? Unfortunately due to special circumstances Immunofluorecence is not an option. Any help is much appreciated. Thanks a lot ! Martin Weber The Scripps Research Institute Dept. of Molecular and Experimental Medicine 10550 North Torey Pines Road La Jolla, 92037 Phone: 858-784-8612 Fax: 858-784-2030 E-Mail: maweber@scripps.edu ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From weneng <@t> hotmail.com Thu Feb 19 16:42:56 2004 From: weneng <@t> hotmail.com (Wendy England) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] angiogenesis study Message-ID: Dear histonetters, We are going to have a study that check angiogenesis after drug treatment. I have three questions: a, what tissue we should look at, is eye a good one? b, how to fix this tissue? c, what stain, or what antibody, we should use? Any advice is highly appreciated. Wendy _________________________________________________________________ Say “good-bye” to spam, viruses and pop-ups with MSN Premium -- free trial offer! http://click.atdmt.com/AVE/go/onm00200359ave/direct/01/ From nick.kirk3 <@t> btopenworld.com Fri Feb 20 00:58:29 2004 From: nick.kirk3 <@t> btopenworld.com (=?iso-8859-1?q?NICK=20KIRK?=) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] angiogenesis study In-Reply-To: Message-ID: <20040220065829.44053.qmail@web86305.mail.ukl.yahoo.com> Hi Wendy If you're checking angiogenisis after drug treatment, surely you should be comparing tissue before and after the drug treatment to validate any change in blood vessel growth due to the drug? In which case eyes would be a bad choice of tissue. To my mind you should be looking at whatever tissue the particular drug effects, especially if it's cytotoxic/anti cancer etc. A few years ago I suppervised a Masters Degree project on angiogenisis in breast carcinomas and CD31 and CD34 seemed to be the best antibodies to demonstrate small blood vessels. EGF (endothelial growth factor) is another one to consider. The tissue used was standard formalin fixed and HIER was used to unmask the epitopes. Best advice I suppose, is to scan the literature to see what the current thinking is. There's been a lot of papers published on angiogenesis in the recent past, so there's plenty of info available. Good luck Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England Wendy England wrote: Dear histonetters, We are going to have a study that check angiogenesis after drug treatment. I have three questions: a, what tissue we should look at, is eye a good one? b, how to fix this tissue? c, what stain, or what antibody, we should use? Any advice is highly appreciated. Wendy _________________________________________________________________ Say ?good-bye? to spam, viruses and pop-ups with MSN Premium -- free trial offer! http://click.atdmt.com/AVE/go/onm00200359ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Walker <@t> sanofi-synthelabo.com Fri Feb 20 04:22:50 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] AP binding Message-ID: Hello, We're trying a new binding technique using Alkaline Phosphatase fusion proteins. This technique makes it possible to do binding experiments with proteins that are unavailble as a radioiodinated form. For initial trials we are using Neurotensin-AP, for which we have radioactive binding tests to verify the AP results. Protocol: Staining of tissue sections : 1. Rehydrate the sections for 10 min in 100 ?l of HBHA. 2. Incubate for 2 hours in the AP fusion Neurotensin at 100 nM diluted in HBS (cover all sections on the slide) at room temperature in a moist chamber. 3. Rinse 4 times in cold HBS. 4. Fix for 15 sec in Acetone-Formaldehyde fixative. 5. Wash the sections 6 times in HBS. 6. Incubate the sections in preheated HBS, in a 65?C water bath, for 15 min. 7. Wash the sections once in AP reaction buffer. 8. Add BCIP/TNBT substrate to cover the sections on the slide. Incubate in the dark at room temperature. Recipe: ? HBHA (Hepes-Buffered Hanks' Solution) : BSA????..0.5 mg/ml NaN3????0.1% (w/v) Hepes????20 mM Adjust pH to 7. Prepare solution in Hanks'. ? HBS (Hepes Buffered Saline) NaCl????.150 mM Hepes????20 mM Adjust pH to 7. Prepare solution in distilled water. ? Acetone-Formaldehyde Fixative : Acetone?????.?.60% (v/v) Formaldehyde????3% (v/v) Hepes???????.20 mM Adjust pH to 7. Prepare solution in distilled water. ? AP Reaction Buffer : Tris-HCl??????100 mM NaCl???????...100 mM MgCl2???????.50 mM Adjust pH to 9.5. Prepare solution in distilled water. BCIP/TNBT : Substrate delivered by CEMICON INTERNATIONAL. First 2 trials we got nothing......... Does anyone have a protocol or suggestions?? thanks and have a good weekend, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From mikael.niku <@t> helsinki.fi Fri Feb 20 04:46:22 2004 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] angiogenesis study In-Reply-To: Message-ID: <000101c3f79e$c835fa60$8c0fd680@ekk1116> Dear Wendy, to recommend a tissue to look at, I think you need to be a bit more specific. Are you expecting a systemic effect on angiogenesis? Or are you going to apply the drug locally and look for local effects? Are you looking for increased angiogenesis, or inhibition? In the latter case, you of course need a tissue where you either have angiogenesis normally (which are not too many in a healthy animal!), or where you can easily induce it. I think mouse or rabbit ear is a common model to study angiogenesis induction. The staining methods recommended by Nick are probably the most commonly used. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ + Mikael Niku + University of Helsinki, Dept. Basic Veterinary Sciences + URL: www.helsinki.fi/~mniku/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! (Gandhi) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of Wendy England > Sent: 20. helmikuuta 2004 0:43 > To: histonet@pathology.swmed.edu > Subject: [Histonet] angiogenesis study > > > Dear histonetters, > > We are going to have a study that check angiogenesis after > drug treatment. > I have three questions: a, what tissue we should look at, is > eye a good one? > b, how to fix this tissue? c, what stain, or what antibody, > we should use? > > Any advice is highly appreciated. > Wendy > > _________________________________________________________________ > Say ?good-bye? to spam, viruses and pop-ups with MSN Premium > -- free trial > offer! http://click.atdmt.com/AVE/go/onm00200359ave/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listin> fo/histonet > From David.Edmondson <@t> christie-tr.nwest.nhs.uk Fri Feb 20 06:51:35 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] while we're talking about von Willebrand Factor... Message-ID: Hi Jack, I wonder that one should not be doing the assay before trying out the system. One assumes that all the components of the Ab stages are OK. You appear to be working nearly blind, so go get DAKOs method book in pdf format if you need the basics, dakocytomation.co.uk is the place this side of the water. dakocytomation.us over there Trypsinisation, for twenty odd years we have been using 40mg of Trypsin in 100ml of 0.1% Calcium Chloride (0.04%)8 to 12 mins digestion, So your 0.25% seems more than strong enough. I wonder at the EDTA, which would appear to chelate any Calcium that might be around as a co-enzyme. Dave Manchester UK -----Original Message----- From: Jack England [mailto:joeamateur@hotmail.com] Sent: 19 February 2004 19:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] while we're talking about von Willebrand Factor... Another REALLY basic question from the Rookie... We're about to do our first vWf assay today, and none of us in this lab have done Ab staining before. We've got primary Ab and secondary Ab, and I'm reasonably certain I can get it to work once I get past the Ag retrieval step. For that, the only enzyme we have in the lab right now is 0.25% trypsin/0.1mM EDTA mix. I've tried to check the references supplied previously, but all require subscriptions that I don't have access to. Here's my question: is that concentration of trypsin adequate for Ag retrieval, and if so, how long would it incubate for (assume 37 degrees)? (For that matter, would the chelative effects of the added EDTA mess things up?) >From what I gather, proteinase K digestion would be the best way to go, but unfortunately time is of the essence and we can't get it fast enough. So the more important question, I guess, is whether I tell my PI that it can't be done with what we have on hand, or whether it can be done (though not perfectly). Can anyone out there advise? --Many, many thanks, Jack England _________________________________________________________________ Dream of owning a home? Find out how in the First-time Home Buying Guide. http://special.msn.com/home/firsthome.armx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> mail.vetmed.lsu.edu Fri Feb 20 07:58:57 2004 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Mast cell staining Message-ID: We do toluidine blue staining regularly. We have found that the biggest cause for poor staining is the pH of the solution. It should be about 2.5. Our solution is simple: Toluidine blue, 0.5 gm; 20% alcohol, 100 ml. Place slides in T blue solution - 2 min. Rinse in water. Differentiate in 2 changes each 95% alcohol and absolute alcohol - 1 min each. Air dry slides. Coverslip. It is important to air dry the slides as the alcohol left on the slides can continue differentiation. Results: Mast cells: blue with magenta granules. Hope this helps. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 From Evelyn.Flynn <@t> childrens.harvard.edu Fri Feb 20 08:45:46 2004 From: Evelyn.Flynn <@t> childrens.harvard.edu (Flynn, Evelyn) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] angiogenesis study Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Wendy England Sent: Thu 2/19/2004 5:42 PM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] angiogenesis study Dear histonetters, We are going to have a study that check angiogenesis after drug treatment. I have three questions: a, what tissue we should look at, is eye a good one? b, how to fix this tissue? c, what stain, or what antibody, we should use? Any advice is highly appreciated. Wendy _________________________________________________________________ Say ?good-bye? to spam, viruses and pop-ups with MSN Premium -- free trial offer! http://click.atdmt.com/AVE/go/onm00200359ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dear Wendy, For about ten years I worked in a lab which studied angiogenesis. The eye (rabbit and mouse) was used with an assay whereby a pellet (usually containing FGF or VEGF) was implanted in the cornea. Otherwise, as part of a study on tumor/tissue effects, vessel counts were done on sections stained with CD31 or CD34. In the past when von Willebrand factor antibody was used, Carnoy's was the best fixative although CD31 antibody will not work on Carnoy's-fixed tissue. Tumors which decreased in volume as a result of anti-angiogenic agents had the same proliferation index as control tumors (counts done sections stained with PCNA or other proliferation antibodies), but a slightly higher index of apoptosis (TUNEL assay), which over time resulted in a smaller tumor volume. Hope this helps, Evelyn Evelyn Flynn Children's Hospital Boston, MA 02115 From labby992000 <@t> yahoo.com Fri Feb 20 12:21:22 2004 From: labby992000 <@t> yahoo.com (linda Ross) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] antibody elution solution Message-ID: <20040220182122.31795.qmail@web21405.mail.yahoo.com> Hi, Does anyone know if there is a commercially available reagent to elute an antibody complex for a doublestaining procedure? I am trying to double label two mouse primarys using a MOM kit. I know that Dako includes a doublestain block in a kit but it is not available by itself. I also called zymed and inquired about their doublestain enhancer. The tech rep said that this product does not remove the complex and could not tell me how it works....just that it might help me. Has anyone used this product before? Thank you....Linda --------------------------------- Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. From weneng <@t> hotmail.com Fri Feb 20 12:24:14 2004 From: weneng <@t> hotmail.com (Wendy England) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Thanks a lot! Message-ID: Thank you all who give me important advice on angiogenesis study. Originally this experiment is for other purpose but later on they also want to check if this drug will induce the angiogenesis. So I was asked which tissue would be the best candidate to look on. I did many literature research and I really appreciated the advice from all expertises of you. Thanks again. Wendy _________________________________________________________________ Take off on a romantic weekend or a family adventure to these great U.S. locations. http://special.msn.com/local/hotdestinations.armx From MAUGER <@t> email.chop.edu Fri Feb 20 12:39:02 2004 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] antibody elution solution Message-ID: Linda, I too would like to buy or make the double stain block myself. A trusted sales rep told me that it is a weak soln. of HCL + methanol. I don't know thw %, but it can't hurt to try. I haven' yet had the chance myself. Jo >>> "linda Ross" 02/20/04 01:21PM >>> Hi, Does anyone know if there is a commercially available reagent to elute an antibody complex for a doublestaining procedure? I am trying to double label two mouse primarys using a MOM kit. I know that Dako includes a doublestain block in a kit but it is not available by itself. I also called zymed and inquired about their doublestain enhancer. The tech rep said that this product does not remove the complex and could not tell me how it works....just that it might help me. Has anyone used this product before? Thank you....Linda --------------------------------- Do you Yahoo!? Yahoo! Mail SpamGuard - Read only the mail you want. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Feb 20 12:52:36 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] re amplification reagent Message-ID: <000a01c3f7e2$b4da70b0$24268551@home> Some people in our Centre regularly use the NEN kit. Mind you, I make my own, using the excellent recipe published by George King a few years ago. Very cheap, very reliable. Costs about ?150 to make and lasts.......ages. Depending on usage of course. From KMMerril <@t> LancasterGeneral.org Fri Feb 20 13:26:35 2004 From: KMMerril <@t> LancasterGeneral.org (Merrill, Kathleen M) Date: Fri Sep 16 15:22:36 2005 Subject: FW: [Histonet] Fw: cornflaking on slides - using sakuratapecoverslipper Message-ID: -----Original Message----- From: Merrill, Kathleen M Sent: Thu 2/19/2004 10:07 AM To: Dave Johnson Cc: Subject: RE: [Histonet] Fw: cornflaking on slides - using sakuratapecoverslipper We turned up the volume of xylene that is dispensed onto the slide, just slightly. That seemed to take care of the problem for us. -----Original Message----- From: Dave Johnson [mailto:djohnson14@hotmail.com] Sent: Thu 2/19/2004 9:32 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: RE: [Histonet] Fw: cornflaking on slides - using sakuratapecoverslipper Have you tried the tape that Mercedes Medical carries. Try calling Chad at 800-331-2716 THanks Dave Johnson Pacific SW Labs www.psl-equip.com >From: srishan@mail.holyname.org >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Fw: cornflaking on slides - using sakura >tapecoverslipper >Date: Thu, 19 Feb 2004 09:22:46 -0500 > > > > > > >----- Forwarded by Nirmala Srishan/HNH on 02/19/2004 09:22 AM ----- > > Nirmala > Srishan/HNH > To > 02/17/2004 10:09 histonet@lists.utsouthwestern.edu > AM cc > > Subject > cornflaking on slides - using > sakura tape coverslipper > > > > > > > > > >Hello, > >We are currently using a tape coverslipper from Sakura. Although this is >not a daily occurrence it might affect 10-15% of the slides on certain >days. We have tried to follow all the remedy given by Sakura and still >having problems. Has anyone else confronted this issue? If so, how was >the issue resolved? > >Thank you >Nirmala Srishan > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get fast, reliable access with MSN 9 Dial-up. Click here for Special Offer! http://click.atdmt.com/AVE/go/onm00200361ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jincan <@t> itsa.ucsf.edu Fri Feb 20 23:04:47 2004 From: jincan <@t> itsa.ucsf.edu (jincan) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] antibody elution solution In-Reply-To: <20040220182122.31795.qmail@web21405.mail.yahoo.com> Message-ID: <5.1.1.6.0.20040220205214.00b1afa8@itsa.ucsf.edu> Hi Linda, Any solution of low pH will work to some extend. The principle is to elute first primary antibody (and thus also the secondary antibodies attached to it) from sections. Many commercial kits use 0.1N HCL. However, I found 0.1M glycine buffer, pH2.0 worked better (5-10 minutes each, two times). The buffered solution is milder to the tissue (in preserving morphology) and will not reveal as many new epitopes or background as 0.1N HCL. Nevertheless, removal of first antibody is hardly complete but only to reduce it to a level that would allow differentiation. Good luck! James Guo Lab Manager ImmunoVision Technologies, Co. 100 North Hill Drive, #32 Brisbane, CA 94005 Tel: 650-302-4622 Fax: 650-558-9621 Website: www.immunovisiontech.com At 10:21 AM 2/20/2004 -0800, you wrote: >Hi, >Does anyone know if there is a commercially available reagent to elute an >antibody complex for a doublestaining procedure? I am trying to double >label two mouse primarys using a MOM kit. I know that Dako includes a >doublestain block in a kit but it is not available by itself. I also >called zymed and inquired about their doublestain enhancer. The tech rep >said that this product does not remove the complex and could not tell me >how it works....just that it might help me. Has anyone used this product >before? Thank you....Linda > > >--------------------------------- >Do you Yahoo!? >Yahoo! Mail SpamGuard - Read only the mail you want. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mark1 <@t> roper74.freeserve.co.uk Sun Feb 22 07:00:38 2004 From: mark1 <@t> roper74.freeserve.co.uk (Mark Roper) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] artery sectioning References: <6.0.0.22.0.20040217093225.025e7808@pop.vt.edu> Message-ID: <000201c3f944$05c08020$3bab4e51@oemcomputer> Hi Laura Another trick to get rid of folds in tricky tissues is float them out on 50% alcohol first. Place a clean slide on the side of the waterbath and pour a few ml of 50% alcohol onto it. Then float the artery section onto this slide and gently lower it into the waterbath. I think the alcohol expands quickly when it hits the warm water - pulling out any creases. Regards Mark Roper CBMS Histopathology Scarborough Hospital England > I am having a difficult time sectioning artery for the HT practical. I > have increased the temperature of my water bath but the folds still > persist. Any suggestions? > > > Laura L. Fidgen, MScF, BSc > Laboratory and Research Practioner > VMRCVM, Laboratory Central Receiving > Blacksburg, VA 24060-0443 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Myri37 <@t> aol.com Sun Feb 22 07:41:44 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] MMA Message-ID: <7DC6C84A.63952D6C.0005167B@aol.com> hello everyone am writting you about MMA embedding i had a protocol using only MMA with peroxide benzoyle, embedding at 27 C?, tissue was preserved, but immunostainnig was impossible and we found another protocol using MMA, methylbenzoate, PEG 400, butylmetacrylate, this one preserves tissue for immunostaining,and it's embedded at -20?C does anyone use the first or the other, wich one is better for light microscopy ? ou do you have other protocols ? i would like to preserve mineralized tissue, and its interface with a metal biomaterial any information or advice would be helpfull Thank you Myriam From int09018 <@t> alphahunt.com Sun Feb 22 11:22:28 2004 From: int09018 <@t> alphahunt.com (HCS) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Fw: Looking for parts, for a shandon embeddeing center older model Message-ID: <001801c3f968$725df0e0$6501a8c0@hp> Hi, my heating unit on the storage side of the embedder has gone out. Does anyone have a Shandon embedding center they would like to sell of perhaps a parts machine. This is the mostly white embedder with the black front. Thanks, LeRoy Brown HT(ASCP) HTL Histology Consultation Services Everson, WA 98247 1-360-966-7300 www.histocs.com From nancymaronto <@t> sbcglobal.net Sun Feb 22 14:20:47 2004 From: nancymaronto <@t> sbcglobal.net (Nancy Maronto) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] MMA Message-ID: <20040222202047.28798.qmail@web80408.mail.yahoo.com> Myriam, I have used the MMA/butylmethacrylate/methylbenzoate/peg 400/ benzoyl peroxide (BPO) combination to embed undecalcified bone and small stented arteries. It is an easy combination to make up and use. I expect you have three changes suggested in your procedure. Mix each about 4 hours before use. It seems I have the best results with this. The infiltration time will have to be worked out and if vacuum is used, it should be limited to a couple of hours after each change of plastic. I make prepolymerized containers to place the tissue on and add the embedding plastic on. It will keep down the bubbles. When embedding, the air in the embedding container with the liquid plastic will need to be flushed out with C02 (easiest to use), you can also use gaseous nitrogen. It takes one to two days for complete polymerization in the freezer. Things that can cause problems with polymerization: Containers not flushed enough that have oxygen in them will not fully polymerize. I place the flushed embedding containers in a bell jar, flush jar with CO2 and seal. This goes in the freezer. Humid conditions can cause poorly polymerization ---some hard--some still liquid. Usually have to replace the embedding plastic, flush with CO2 and try again. You can use drierite in the bottom to help this. After the blocks are hard, the plastic is trimmed a little to the block size I want to use in the microtome. I wet cut 4 micron sections and place them on a chrome-gelatin coated slide with a couple of drops of 70% alcohol, on a slide warmer. The section flattens out, I put a piece of plastic on it, gently roll with a small wall paper roller. when I get them done I clamp them with c-clamps. It is good idea to have a small piece of wood and extra glass slide on each end. It prevents slides from breaking. They get placed in the oven overnight. The clamps removed, plastic removed, they are ready to deplasticize with xylene and ready for staining. You may want to deplasticize with other agents such as 2-methoxyethyl acetate for immunostaining. Your procedure may give you more info for immunostaining. I remember doing Brdu with success, however, we would get a halo effect around the possitive cells, and I could not find anyone that had a solution to this. I did find that certain antibody diluents and blockers were a key in the success of this. I hope this helps. Nancy MPI Research Mattawan, MI From v.lamanna <@t> abdn.ac.uk Mon Feb 23 04:09:13 2004 From: v.lamanna <@t> abdn.ac.uk (Vincenzo La Manna) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] primers ER alpha & beta, PR, TGFbeta Message-ID: <5.2.1.1.0.20040223100702.00aa1d88@mailms.abdn.ac.uk> Hi Histonetters, I'm looking for primers to ER alpha & beta, PR & TGFbeta for RT PCR in bovine tissue. First time I try PCR :-( so, I would be very pleased about any suggestion, reference or help from anyone! thanks in advance, ------------ Vincenzo La Manna Hilton Place, block M, AB24 4FA, Aberdeen Tel. 0044 (0)1224 274259 Fax. 0044 (0)1224 273731 Mobile 0044 (0)7952 637728 e-mail: v.lamanna@abdn.ac.uk From c.m.vanderloos <@t> amc.uva.nl Mon Feb 23 04:30:27 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Re: antibody elution solution Message-ID: <1d11c61d1ff4.1d1ff41d11c6@amc.uva.nl> Hi, I fully agree with James that 0.1M glycine buffer pH2.0 may work well for eluting bound antibodies from a tissue specimen for double staining purposes. Indeed the removal of the first antibody is hardly complete, not to say isn't working at all! Using glycine-HCl buffer you may remove your second step reagent from a tissue section, but especially high affinity antibodies just stay nicely bound at the antigen in the tissue section. Problem is that the affinity characteristic of a primary antibody isn't mentioned in data sheets! Long time ago investigators tried many ways to get rid of the first set of antibodies and leaving the enzymatic reaction product. Lots of chemicals were tested including oxidation of antibodies and even a kind of electrophoresis system. All procedures shared the problem of high affinity primaries that stayed on the tissue section. There is some older literature available on this point. The only way I came across for excellent removal of antibodies is a short heat-induced antigen retrieval method using citrate6.0 (Lan et al. JHC 43:97-102, 1985). Indeed not a single antibody will survive cooking! Obviously this can be done for FFPE sections only, not for cryo's! The story above doesn't say that sequential double staining isn't working at all. When applying DAB in the first staining sequence the reaction product shelters the first set of immunoreagents quite effectively. The second primary and second set of detection reagents can no longer cross-react with the first immunostaining sequence. This characteristic makes the DAB reaction product suitable for at least double staining two different cell types. However, if you wish to look at mixed-colors marking co-localization of two antigens at one cell, the sequential double staining using DAB in the first sequence is not safe. In this case you need to consider other double staining systems! Chris van der Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From jincan Date Fri, 20 Feb 2004 21:04:47 -0800 To linda Ross Cc Histonet@lists.utsouthwestern.edu Subject Re: [Histonet] antibody elution solution Hi Linda, Any solution of low pH will work to some extend. The principle is to elute first primary antibody (and thus also the secondary antibodies attached to it) from sections. Many commercial kits use 0.1N HCL. However, I found 0.1M glycine buffer, pH2.0 worked better (5-10 minutes each, two times). The buffered solution is milder to the tissue (in preserving morphology) and will not reveal as many new epitopes or background as 0.1N HCL. Nevertheless, removal of first antibody is hardly complete but only to reduce it to a level that would allow differentiation. Good luck! James Guo Lab Manager ImmunoVision Technologies, Co. 100 North Hill Drive, #32 Brisbane, CA 94005 Tel: 650-302-4622 Fax: 650-558-9621 Website: www.immunovisiontech.com At 10:21 AM 2/20/2004 -0800, you wrote: >Hi, >Does anyone know if there is a commercially available reagent to elute an >antibody complex for a doublestaining procedure? I am trying to double >label two mouse primarys using a MOM kit. I know that Dako includes a >doublestain block in a kit but it is not available by itself. I also >called zymed and inquired about their doublestain enhancer. The tech rep >said that this product does not remove the complex and could not tell me >how it works....just that it might help me. Has anyone used this product >before? Thank you....Linda From daniel.eberhard <@t> uni-bielefeld.de Mon Feb 23 04:22:51 2004 From: daniel.eberhard <@t> uni-bielefeld.de (DANIEL EBERHARD) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] ki67 or pcna (anti-mouse) Message-ID: <4039D47B.7000604@UNI-BIELEFELD.DE> Dear All, sorry if this question has been ask before, I?m searching for a ki67 or pcna (anti-mouse) antibody which works on pFA fixed tissue. Thanks for any hints. Daniel. -- (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V= Developmental Biology & Molecular Pathology Graduate Programe on Pattern Formation University of Bielefeld D 33501 Bielefeld/Germany FAX: xx49(0)521-106-5654 (-)-(-) --------------------------- \"/ --- =V= From barbara.bublava <@t> meduniwien.ac.at Mon Feb 23 07:23:28 2004 From: barbara.bublava <@t> meduniwien.ac.at (Ing. Barbara Bublava) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Cryosectioning of Lung Message-ID: <000501c3fa10$3963fa20$9865fea9@GERICHTS9XOZZ8> Hi everybody, I?m having problems with the cryosectioning of lung, the air in it causes some trouble. I had the idea to get rid of it using vaccum but our pathologist thinks that this woul affekt oedemas. I have to section formalin-fixated and native lung. I did try to work at -20?C for native and -30?C for fixated lung. I would be very glad for any hints. Thanx in advance Barbara Bublava Inst. f. Forensic Medicine Vienna From cfavara <@t> niaid.nih.gov Mon Feb 23 07:21:49 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] GFAP raised in goat Message-ID: I am trying to do a double stain with GFAP and an in house AB that has been raised in rabbit. I have found GFAP raised in mouse and chicken but wonder if anyone knows of any GFAP raised in goat? Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 From ames1 <@t> breathe.com Mon Feb 23 08:48:13 2004 From: ames1 <@t> breathe.com (ames1@breathe.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Cryostat sections Message-ID: Hi all, I am experiencing problems with my cryostat sections. They keep floating off the slide when I am using antibody staining. I have tried removing the acetone for fixing, but that makes no difference. My slides are the same as I have always used and I was in the middle of a pack when this problem surfaced. I was wondering if anyone had any tips on how to keep the sections on the slide during staining. Thanks Amy Greenhalgh Scientist Sequani Ltd Bromyard Rd Ledbury HR8 1LH From a-lisowski <@t> northwestern.edu Mon Feb 23 08:51:15 2004 From: a-lisowski <@t> northwestern.edu (a-lisowski@northwestern.edu) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Destaing H&E Message-ID: <200402231451.i1NEpUwf027054@hecky.it.northwestern.edu> What is the simple method of destaining H&E slides? Most of the precious slides are too dark.. thanks Andrew From ames1 <@t> breathe.com Mon Feb 23 08:56:05 2004 From: ames1 <@t> breathe.com (ames1@breathe.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] re:cryostat sections Message-ID: Hi All, Sorry should have said I'm using positive charge slides. Thanks Amy Greenhalgh Scientist Sequani Ltd Bromyard Rd Ledbury HR8 1LH From garygill <@t> dcla.com Mon Feb 23 09:07:05 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Destaing H&E Message-ID: After removing the cover glass, proceed as follows: No. Solution Time Note 1-2 Xylene 1 min/each Removes traces of mountant. Dip repeatedly; inspect surface. A wavy rather than a smoothly glistening surface denotes incomplete rinsing and indicates further dipping. 3-5 Abs. EtOH 1 min/each Prepares slides for next step. 6 1.0% HCl* min-1 hr Removes hematoxylin. Monitor the progress of decolorization by periodic microscopic inspection. 7 1.5% NH4OH in 70% ethanol** 1 min Removes eosin. Time required may vary and should be adjusted as needed. Repeated dipping aids uniform decolorization. 8-9 Tap water 1 min/each To H&E stain * 0.23 mL HCl concentrated (i.e., 36.5-38% w/w, S.G. 1.1854-1.1923, or 5.5 mL N/2 HCl) in water q.s. 100 mL ** 5.7 mL NH4OH concentrated, 29.2% w/w, S.G. 0.900, in 75 mL 95% ethanol, and water q.s. 100 mL Gary Gill -----Original Message----- From: a-lisowski@northwestern.edu [mailto:a-lisowski@northwestern.edu] Sent: Monday, February 23, 2004 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destaing H&E What is the simple method of destaining H&E slides? Most of the precious slides are too dark.. thanks Andrew _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stephen.Eyres <@t> sanofi-synthelabo.com Mon Feb 23 09:27:50 2004 From: Stephen.Eyres <@t> sanofi-synthelabo.com (Stephen.Eyres@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Users of Leica slide writer Message-ID: Hi, We are looking at purchasing a slide writer this year. We had a demo of the Leica instrument last year, and was impressed. However, the laser flash was irritating, but users may get use to that. What are the feelings of current users? I'd be particulary interested in Pharmaceutical and CRO users ways of working with slidewriters generally on how they accommodate the need for recuts and processing additional samples. Thanks Steve "Favara, Cynthia (NIH/NIAID)" To: "HistoNet Server (E-mail)" Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] GFAP raised in goat western.edu 23/02/2004 13:21 I am trying to do a double stain with GFAP and an in house AB that has been raised in rabbit. I have found GFAP raised in mouse and chicken but wonder if anyone knows of any GFAP raised in goat? Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Mon Feb 23 09:46:11 2004 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Cryosectioning of Lung Message-ID: The next issue of HistoLogic will include a comprehensive article by Gayle Callis on the handling of tissues intended for cryosectioning. Gayle may respond to this query herself but have you considered perfusing the lung with OCT before freezing? The OCT can, in this manner, occupy the spaces filled with air that will provide the support the tissue needs during sectioning. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Ing. Barbara Bublava" 02/23/04 08:23AM >>> Hi everybody, I m having problems with the cryosectioning of lung, the air in it causes some trouble. I had the idea to get rid of it using vaccum but our pathologist thinks that this woul affekt oedemas. I have to section formalin-fixated and native lung. I did try to work at -20?C for native and -30?C for fixated lung. I would be very glad for any hints. Thanx in advance Barbara Bublava Inst. f. Forensic Medicine Vienna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ames1 <@t> breathe.com Mon Feb 23 10:40:53 2004 From: Ames1 <@t> breathe.com (Ames1@breathe.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Re: Ki 67 or PCNA (antimouse) Message-ID: Hi Daniel, BD Biosciences Pharmingen do PCNA antibody for the mouse, The contact in Germany is (49) 6221 305 551. We have recently used this antibody for mouse guts and liver, it worked well. Hope this helps. Amy Greenhalgh Scientist Sequani Ltd Bromyard Road Ledbury HR8 1LH From lizchlipala <@t> premierhistology.com Mon Feb 23 10:48:22 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] ki67 or pcna (anti-mouse) In-Reply-To: <4039D47B.7000604@UNI-BIELEFELD.DE> Message-ID: <000d01c3fa2c$dd867f30$74d48a80@LIZ> Daniel Dako has a mouse anti Ki-67 (clone TEC-3) that works well in formalin fixed paraffin embedded tissue. Steam retrieval, primary antibody 1:50 for 30 minutes room temp, then Dako's Biotinylated Rabbit anti-rat at 1:200 for 30 minutes, then streptavidin/HRP for 30 minutes, if you find you need more amplification you can substitute the streptavidin/HRP with Dako's Envision + rabbit, but you might need to change the dilution of the primary. Hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL EBERHARD Sent: Monday, February 23, 2004 3:23 AM To: histonet@pathology.swmed.edu Subject: [Histonet] ki67 or pcna (anti-mouse) Dear All, sorry if this question has been ask before, I?m searching for a ki67 or pcna (anti-mouse) antibody which works on pFA fixed tissue. Thanks for any hints. Daniel. -- (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V= Developmental Biology & Molecular Pathology Graduate Programe on Pattern Formation University of Bielefeld D 33501 Bielefeld/Germany FAX: xx49(0)521-106-5654 (-)-(-) --------------------------- \"/ --- =V= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Mon Feb 23 11:40:53 2004 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] ki67 or pcna (anti-mouse) References: <4039D47B.7000604@UNI-BIELEFELD.DE> Message-ID: <00be01c3fa34$2f8032c0$27955c82@patho.unibe.ch> Hi Daniel I assume you want to stain (para)formaldehyde-fixed, paraffin-embedded mouse tissue, right? Try rat-anti-mouse Ki-67, clone TEC-3 (DakoCytomation M 7249). We use it after HIER in a pressure cooker with citrate buffer, pH 6.0. Working dilution is 1:100 - 1:200, when using a 3-step protocol like ABC, LSAB or similar. Visualization: use a x-a-rat Ig/Biotin secondary that has been absorbed with mouse Ig to avoid demonstration of endogenous mouse Ig in your tissue sections (e.g. DakoCytomation E 0468 at 1:300 - no, I don't have any shares). Good luck Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "DANIEL EBERHARD" To: Sent: Monday, February 23, 2004 11:22 AM Subject: [Histonet] ki67 or pcna (anti-mouse) > Dear All, > sorry if this question has been ask before, > I?m searching for a ki67 or pcna (anti-mouse) antibody > which works on pFA fixed tissue. > Thanks for any hints. > Daniel. > > -- > (-)-(-) > --------------------------- \"/ --- > Dr. Daniel Eberhard =V= > > Developmental Biology > & Molecular Pathology > > Graduate Programe on Pattern Formation > > University of Bielefeld > D 33501 Bielefeld/Germany > > FAX: xx49(0)521-106-5654 (-)-(-) > --------------------------- \"/ --- > =V= > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From padunnje <@t> iupui.edu Mon Feb 23 12:07:14 2004 From: padunnje <@t> iupui.edu (Dunn-Jena, Patsy A) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Would Recruiter who had opening in Indianapolis, IN Message-ID: Please contact me privately at padunnje@hotmail.com. I would appreciate it. Patsy Dunn-Jena padunnje@hotmail.com From bicresource <@t> hotmail.com Mon Feb 23 12:19:11 2004 From: bicresource <@t> hotmail.com (* B.I.C*) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Available Leitz 1400 Sledge Microtome Message-ID: Leitz 1400 Sledge Microtome in excellent condition. Looking for best offer. Mike MacDougall (800)783-9424 Ext.114 _________________________________________________________________ Dream of owning a home? Find out how in the First-time Home Buying Guide. http://special.msn.com/home/firsthome.armx From DCoulter <@t> Lifespan.org Mon Feb 23 12:23:11 2004 From: DCoulter <@t> Lifespan.org (Coulter, Diane) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Using Poly-L-Lysine to coat slides Message-ID: We're presently a commercially prepared, liquid Poly-L-Lysine as an adhesive for some of our slides. Is anyone using a dry form to make up their own solution? If so, where are you purchasing from and what concentration do you use? (We use these slides for special stains.) Thanks in advance, Diane Diane Coulter, RIH Histology From c.m.vanderloos <@t> amc.uva.nl Mon Feb 23 12:37:26 2004 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] RE: ki67 or pcna (anti-mouse) Message-ID: <3958ab398a63.398a633958ab@amc.uva.nl> Daniel, During the Animal Research Kit workshop at NSH 2003 in Louisville last October I used a wonderful Ki67 antibody named CDC47 from Neomarkers. It worked wonderful on FFPE mouse intestinal tissue sections after EDTA9.0 HIER with all workshop attendees! Because CDC47 is raised in mouse, you need the ARKit for detection. Hope this helps. Chris vand er Loos, PhD Dept. of Pathology Academical Medical Center Amsterdam - The Netherlands -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL EBERHARD Sent: Monday, February 23, 2004 3:23 AM To: histonet@pathology.swmed.edu Subject: [Histonet] ki67 or pcna (anti-mouse) Dear All, sorry if this question has been ask before, I?m searching for a ki67 or pcna (anti-mouse) antibody which works on pFA fixed tissue. Thanks for any hints. Daniel. (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V= Developmental Biology & Molecular Pathology Graduate Programe on Pattern Formation University of Bielefeld D 33501 Bielefeld/Germany FAX: xx49(0)521-106-5654 From ramona.tolliver <@t> yale.edu Mon Feb 23 12:47:49 2004 From: ramona.tolliver <@t> yale.edu (Ramona Tolliver) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Yale University - Opening Message-ID: <5.2.0.9.2.20040223134732.0122d248@email.med.yale.edu> Good afternoon, Yale University has a 2nd Shift Histology Supervisor Position available from 3:00 -11:00 p.m. If you know of anyone who may be interested in a great opportunity with advancement potential, please forward this information on to them. The posting is located at : http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=124471. Salary starts at 65K and is commensurate with experience. Relocation assistance is available. Thank you for your time! Ramona Tolliver Ramona E. Tolliver Human Resource Manager Yale University School of Medicine Department of Pathology 310 Cedar Street New Haven, CT 06520-8023 Telephone: (203) 785-6689 Fax: (203) 785-7303 From WesterM <@t> MedImmune.com Mon Feb 23 15:19:47 2004 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] TRAP stain components Message-ID: <83899F0EC7671543B305FB5694024DE6BAD2BE@medimmune4.medimmune.com> I am gearing up to do some TRAP staining, over a period of time. Can anyone tell me if it is possible to make up and store the various stock solutions, how to store them and for what period of time they remain usable? It would help me out a great deal not to have to devote 1-2 hrs just before every incubation period to make up fresh stocks. Thanks for your help! Martha S. Wester Associate Scientist MedImmune, Inc. Gaithersburg, MD 20878 (240) 632-4794 From DOOLEEO <@t> shands.ufl.edu Mon Feb 23 15:43:32 2004 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Perforin Message-ID: Dear histonetters, Does anyone out there have a antibody called Perforin that works well in formalin fixed paraffin embedded tissues? Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 From DOOLEEO <@t> shands.ufl.edu Mon Feb 23 15:43:32 2004 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Perforin Message-ID: Dear histonetters, Does anyone out there have a antibody called Perforin that works well in formalin fixed paraffin embedded tissues? Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 From ramona.tolliver <@t> yale.edu Mon Feb 23 15:48:22 2004 From: ramona.tolliver <@t> yale.edu (Ramona Tolliver) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Yale University - Opening Message-ID: <6.0.1.1.2.20040223164739.01dca2b8@email.med.yale.edu> I'm resending this due to trouble with the URL for the position. It can also be reached by going to www.yale.edu/jobs and searching Pathology. Thanks, Ramona ~~~~~~~~~~~ Good afternoon, Yale University has a 2nd Shift Histology Supervisor Position available from 3:00 -11:00 p.m. If you know of anyone who may be interested in a great opportunity with advancement potential, please forward this information on to them. The posting is located at : http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=151919. Salary starts at 65K and is commensurate with experience. Relocation assistance is available. Thank you for your time! Ramona Tolliver Ramona E. Tolliver Human Resource Manager Yale University School of Medicine Department of Pathology 310 Cedar Street New Haven, CT 06520-8023 Telephone: (203) 785-6689 Fax: (203) 785-7303 From marilyn.johnson <@t> gov.ab.ca Mon Feb 23 15:55:05 2004 From: marilyn.johnson <@t> gov.ab.ca (marilyn.johnson@gov.ab.ca) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] CMC mounting media Message-ID: Hi Histonetters, I am looking for a "CMC" combined stain/mounting medium. Apparently, this soln. can be pretinted with acid fuchsin or aniline blue to the mounting medium. This soln. is required to demonstrate minute radulae. Any replies would be greatly appreciated. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada From j.browne <@t> auckland.ac.nz Mon Feb 23 17:46:18 2004 From: j.browne <@t> auckland.ac.nz (Jeremy Browne) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] unsubscribe please In-Reply-To: <20040222202047.28798.qmail@web80408.mail.yahoo.com> Message-ID: <006401c3fa67$3ba6d2b0$91c7d882@ssc.auckland.ac.nz> 4th attempt!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Maronto Sent: Monday, February 23, 2004 9:21 AM To: histonet@lists.utsouthwestern.edu; Myri37@aol.com Subject: Re: [Histonet] MMA Myriam, I have used the MMA/butylmethacrylate/methylbenzoate/peg 400/ benzoyl peroxide (BPO) combination to embed undecalcified bone and small stented arteries. It is an easy combination to make up and use. I expect you have three changes suggested in your procedure. Mix each about 4 hours before use. It seems I have the best results with this. The infiltration time will have to be worked out and if vacuum is used, it should be limited to a couple of hours after each change of plastic. I make prepolymerized containers to place the tissue on and add the embedding plastic on. It will keep down the bubbles. When embedding, the air in the embedding container with the liquid plastic will need to be flushed out with C02 (easiest to use), you can also use gaseous nitrogen. It takes one to two days for complete polymerization in the freezer. Things that can cause problems with polymerization: Containers not flushed enough that have oxygen in them will not fully polymerize. I place the flushed embedding containers in a bell jar, flush jar with CO2 and seal. This goes in the freezer. Humid conditions can cause poorly polymerization ---some hard--some still liquid. Usually have to replace the embedding plastic, flush with CO2 and try again. You can use drierite in the bottom to help this. After the blocks are hard, the plastic is trimmed a little to the block size I want to use in the microtome. I wet cut 4 micron sections and place them on a chrome-gelatin coated slide with a couple of drops of 70% alcohol, on a slide warmer. The section flattens out, I put a piece of plastic on it, gently roll with a small wall paper roller. when I get them done I clamp them with c-clamps. It is good idea to have a small piece of wood and extra glass slide on each end. It prevents slides from breaking. They get placed in the oven overnight. The clamps removed, plastic removed, they are ready to deplasticize with xylene and ready for staining. You may want to deplasticize with other agents such as 2-methoxyethyl acetate for immunostaining. Your procedure may give you more info for immunostaining. I remember doing Brdu with success, however, we would get a halo effect around the possitive cells, and I could not find anyone that had a solution to this. I did find th at certain antibody diluents and blockers were a key in the success of this. I hope this helps. Nancy MPI Research Mattawan, MI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linresearch <@t> aol.com Mon Feb 23 19:50:44 2004 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] (no subject) Message-ID: <1e9.19a5a825.2d6c07f4@aol.com> Hello, I am still trying to find a CD21 that works on FFPE rat tissues. I would appreciate any suggestions. Lin From Linresearch <@t> aol.com Mon Feb 23 19:52:24 2004 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] CD21 Message-ID: <8a.4306d47.2d6c0858@aol.com> Hello, I am still tryinmg to find a CD21 that works on FFPE rat tissues. Any suggestions would be appreciated. Lin From shaumik.adhya <@t> ucl.ac.uk Tue Feb 24 04:16:44 2004 From: shaumik.adhya <@t> ucl.ac.uk (Shaumik Adhya) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] cutting frozen sections of myocardium Message-ID: <1077617804.403b248cc5e70@www.webmail.ucl.ac.uk> Hi Histonetters, I'm a clinician trying to get to grips with histotechnology techniques for research. I'm having a lot of difficulty cutting frozen sections of rat myocardium. I seem to be cutting sections with a lot of holes in them, and the fibres seem poorly arrayed. I'm cutting rat myocardium to practice before human myocardial biopsies. The rats are killed, the hearts harvested, without fixation. They're cut into small pieces before staining for 20 hours in senescence associated beta galactosidase solution (essentially x-gal solution at pH 6). They are then moutned into OCT, frozen in isopentane and cut on a cryotome at -20 centigrade. The slides are then fixed in 3% paraformaldehyde, wawshed in distilled water, before Haemotxylin & Eosin staining. Any advice, tips or tricks will be much appreciated. Shaumik Adhya From Nancy.Walker <@t> sanofi-synthelabo.com Tue Feb 24 05:46:29 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] filtering stains (what paper?) Message-ID: What grade of filter paper is good for filtering hemalun? The paper we recently bough(Whatman 113V) seems to retain too much (very slow filtering with rapid loss of staining quality). How many times do you refilter stains before discarding? thanks again, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From M.Bromley <@t> dgri.scot.nhs.uk Tue Feb 24 06:51:30 2004 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] substance p Message-ID: <7325D637DFE2D211928800902733A7F303B94A40@DGAMTBDCEMS> Hi All Has anyone immunostained for substance P on formalin fixed paraffins. I would appreciate info re: antibody type and source, retrieval etc Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > From NSEARCY <@t> swmail.sw.org Tue Feb 24 06:51:28 2004 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Isopentane Message-ID: <04Feb24.065158cst.119053@healthcare2.sw.org> For Vinny- or anyone. I am trying to substitute liquid nitrogen for Isopentane in the frozen lab due to volatility. Could not access Histologic article from May regarding laboratory accident. Anyone have any other incidents? Help would be appreciated. From Nancy.Walker <@t> sanofi-synthelabo.com Tue Feb 24 07:23:50 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] filtering stains (what paper?) Message-ID: ----- R?achemin? par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04 14:14 ----- Nancy Walker Pour : histonet@lists.utsouthwestern.edu 24/02/04 12:46 cc : Objet : filtering stains (what paper?)(Document link: Nancy Walker) What grade of filter paper is good for filtering hemalun? The paper we recently bough(Whatman 113V) seems to retain too much (very slow filtering with rapid loss of staining quality). How many times do you refilter stains before discarding? thanks again, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From Myri37 <@t> aol.com Tue Feb 24 07:37:17 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] (no subject) Message-ID: <0F0551F2.71392D2B.0005167B@aol.com> hello i'ev heard of villanueva stain, that is a good stain for bonea do you have any protocol, how to prepare it ? or where could i buy it ? thank you very much Myriam From garygill <@t> dcla.com Tue Feb 24 07:45:00 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] filtering stains (what paper?) Message-ID: Filtration per se does not cause "rapid loss of staining quality" -- regardless of pore size. However, the "very slow filtering" you've described suggests you're using Harris hematoxylin, which typically throws a surface precipitate when exposed to atmospheric oxygen. That precipitate is actually aluminum-hematein, which is the active complex responsible for staining. It forms because oxygen continues to oxidize initially unoxidized hematoxylin, which forms so much more aluminum-hematein that it exceeds its solubility limit in water. Repeatedly removing this complex by filtering over-and-over again causes the rapid loss of staining quality. Mixing three parts of Harris hematoxylin with 1 part ethylene glycol fixes the problem. Aluminum-hematein is several times more soluble in 20% ethylene glycol than it is in water. Gary Gill -----Original Message----- From: Nancy.Walker@sanofi-synthelabo.com [mailto:Nancy.Walker@sanofi-synthelabo.com] Sent: Tuesday, February 24, 2004 8:24 AM To: Histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] filtering stains (what paper?) ----- R?achemin? par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04 14:14 ----- Nancy Walker Pour : histonet@lists.utsouthwestern.edu 24/02/04 12:46 cc : Objet : filtering stains (what paper?)(Document link: Nancy Walker) What grade of filter paper is good for filtering hemalun? The paper we recently bough(Whatman 113V) seems to retain too much (very slow filtering with rapid loss of staining quality). How many times do you refilter stains before discarding? thanks again, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garygill <@t> dcla.com Tue Feb 24 07:45:00 2004 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] filtering stains (what paper?) Message-ID: Filtration per se does not cause "rapid loss of staining quality" -- regardless of pore size. However, the "very slow filtering" you've described suggests you're using Harris hematoxylin, which typically throws a surface precipitate when exposed to atmospheric oxygen. That precipitate is actually aluminum-hematein, which is the active complex responsible for staining. It forms because oxygen continues to oxidize initially unoxidized hematoxylin, which forms so much more aluminum-hematein that it exceeds its solubility limit in water. Repeatedly removing this complex by filtering over-and-over again causes the rapid loss of staining quality. Mixing three parts of Harris hematoxylin with 1 part ethylene glycol fixes the problem. Aluminum-hematein is several times more soluble in 20% ethylene glycol than it is in water. Gary Gill -----Original Message----- From: Nancy.Walker@sanofi-synthelabo.com [mailto:Nancy.Walker@sanofi-synthelabo.com] Sent: Tuesday, February 24, 2004 8:24 AM To: Histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] filtering stains (what paper?) ----- R?achemin? par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04 14:14 ----- Nancy Walker Pour : histonet@lists.utsouthwestern.edu 24/02/04 12:46 cc : Objet : filtering stains (what paper?)(Document link: Nancy Walker) What grade of filter paper is good for filtering hemalun? The paper we recently bough(Whatman 113V) seems to retain too much (very slow filtering with rapid loss of staining quality). How many times do you refilter stains before discarding? thanks again, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Tue Feb 24 10:10:18 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: [Histonet] cutting frozen sections of myocardium Message-ID: <6.0.1.1.0.20040224100656.025955a0@mailhost.vetmed.auburn.edu> Hello is it possible for you to fix overnight or for 24hrs and then cryoprotect in sucrose/fixative or sucrose/PB before freezing for cryosectioning? If you know that fixation won't destroy the enzyme you might try this method. Best wishes. Atoska >Date: Tue, 24 Feb 2004 10:16:44 +0000 >From: Shaumik Adhya >To: histonet@lists.utsouthwestern.edu >User-Agent: Internet Messaging Program (IMP) 3.2.1 >X-Originating-IP: 128.40.253.73 >X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, > helpdesk@ucl.ac.uk for more information >X-UCL-MailScanner: Found to be clean >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 05628b3a735271e1629bc35bb8abda07 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] cutting frozen sections of myocardium >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes > >Hi Histonetters, > >I'm a clinician trying to get to grips with histotechnology techniques for >research. I'm having a lot of difficulty cutting frozen sections of rat >myocardium. I seem to be cutting sections with a lot of holes in them, and >the fibres seem poorly arrayed. I'm cutting rat myocardium to practice >before >human myocardial biopsies. > >The rats are killed, the hearts harvested, without fixation. They're cut >into >small pieces before staining for 20 hours in senescence associated beta >galactosidase solution (essentially x-gal solution at pH 6). They are then >moutned into OCT, frozen in isopentane and cut on a cryotome at -20 >centigrade. The slides are then fixed in 3% paraformaldehyde, wawshed in >distilled water, before Haemotxylin & Eosin staining. > >Any advice, tips or tricks will be much appreciated. > >Shaumik Adhya > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From dennijc <@t> vetmed.auburn.edu Tue Feb 24 10:22:25 2004 From: dennijc <@t> vetmed.auburn.edu (John C. Dennis) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] substance p In-Reply-To: <7325D637DFE2D211928800902733A7F303B94A40@DGAMTBDCEMS> Message-ID: Mike I used a rabbit polyclonal from Chemicon. It worked well. John Carroll Dennis Anatomy, Physiology, and Pharmacology 109 Greene Hall Auburn University, AL 36849 On Tue, 24 Feb 2004, Mike Bromley wrote: > Hi All > Has anyone immunostained for substance P on formalin fixed paraffins. I > would appreciate info re: antibody type and source, retrieval etc > > Best Wishes > > Mike Bromley > > Chief Biomedical Scientist > Pathology > Dumfries & Galloway Royal Infirmary > Scotland, UK > > > This e-mail and any files transmitted with it are private and intended > > solely for the use of the individual or entity to whom they are addressed. > > If you have received this e-mail in error please return it to the address > > it > > came from telling them it is not for you and then delete it from your > > system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gentras <@t> vetmed.auburn.edu Tue Feb 24 10:12:15 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: [Histonet] Isopentane Message-ID: <6.0.1.1.0.20040224101051.0259e070@mailhost.vetmed.auburn.edu> Hello, I don't believe isopentane can be substituted for liquid nitrogen, they're usually used in conjunction. However, dry ice can be substituted for liquid nitrogen. Best wishes. Atoska >X-Mailer: Novell GroupWise Internet Agent 6.5.1 >Date: Tue, 24 Feb 2004 06:51:28 -0600 >From: "Nita Searcy" >To: >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.3 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] Isopentane >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63 >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) >X-SA-Exim-Scanned: Yes > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for >Isopentane in the frozen lab due to volatility. Could not access >Histologic article from May regarding laboratory accident. Anyone >have any other incidents? Help would be appreciated. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From mward <@t> wfubmc.edu Tue Feb 24 11:02:30 2004 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] please unsubscribe Message-ID: <61135F0455D33347B5AAE209B903A304076A4DD5@EXCHVS2.medctr.ad.wfubmc.edu> I will out of the lab from 2/24/2004 to 3/8/2004 Martha Ward Wake Forest University Baptist Medical Center From gentras <@t> vetmed.auburn.edu Tue Feb 24 11:23:43 2004 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: Re: Fwd: [Histonet] Isopentane Message-ID: <6.0.1.1.0.20040224112043.025921c0@mailhost.vetmed.auburn.edu> Hello, I'm not aware of a procedure for quick freezing in isopentane alone, we quick freeze by immerging the sample in isopentane chilled by liquid nitrogen. Please will you share with me your protocol for quick freezing in isopentane. Thanks, Atoska >X-Mailer: Novell GroupWise Internet Agent 6.5.1 >Date: Tue, 24 Feb 2004 10:50:58 -0600 >From: "Nita Searcy" >To: >Subject: Re: Fwd: [Histonet] Isopentane > >Isopentane is used to "quick freeze" frozen sections- can't liquid >nitrogen be use in its place? No specialty work (muscles, etc.) but >routine frozens. > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > >>> > >Hello, I don't believe isopentane can be substituted for liquid >nitrogen, >they're usually used in conjunction. However, dry ice can be >substituted >for liquid nitrogen. Best wishes. Atoska > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > >From: "Nita Searcy" > >To: > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to histotechnology >and > > related fields > >List-Unsubscribe: > >, > > > > > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: >, > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] Isopentane > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no >version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > >Isopentane in the frozen lab due to volatility. Could not access > >Histologic article from May regarding laboratory accident. Anyone > >have any other incidents? Help would be appreciated. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From Barry.R.Rittman <@t> uth.tmc.edu Tue Feb 24 11:40:54 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: [Histonet] Isopentane Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FCA4@UTHEVS3.mail.uthouston.edu> The purpose of using isopentane cooled with liquid nitrogen is to dissipate heat quickly and thus prevent the formation of a vapor barrier around the tissues. When nitrogen is used alone, the vapor barrier that is formed significantly decreases the rate of cooling and therefore increases the possibility of ice crystal formation and tissue damage. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry Sent: Tuesday, February 24, 2004 11:24 AM To: Histonet Subject: Fwd: Re: Fwd: [Histonet] Isopentane Hello, I'm not aware of a procedure for quick freezing in isopentane alone, we quick freeze by immerging the sample in isopentane chilled by liquid nitrogen. Please will you share with me your protocol for quick freezing in isopentane. Thanks, Atoska >X-Mailer: Novell GroupWise Internet Agent 6.5.1 >Date: Tue, 24 Feb 2004 10:50:58 -0600 >From: "Nita Searcy" >To: >Subject: Re: Fwd: [Histonet] Isopentane > >Isopentane is used to "quick freeze" frozen sections- can't liquid >nitrogen be use in its place? No specialty work (muscles, etc.) but >routine frozens. > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > >>> > >Hello, I don't believe isopentane can be substituted for liquid >nitrogen, they're usually used in conjunction. However, dry ice can be >substituted >for liquid nitrogen. Best wishes. Atoska > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > >From: "Nita Searcy" > >To: > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to > >histotechnology >and > > related fields > >List-Unsubscribe: > >, > > > > >> > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: >, > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] Isopentane > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no >version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > >Isopentane in the frozen lab due to volatility. Could not access > >Histologic article from May regarding laboratory accident. Anyone > >have any other incidents? Help would be appreciated. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Tue Feb 24 11:52:45 2004 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] substance p Message-ID: Hello Mike, I have stained for Substance P using a company I had never used before; The Arnel Products Co. P. O. Box 20045/ New York, NY 10021 800-333-6078 I used it with DakoCytomation's ready to use Prot. K for 5 min. at room temp. The dilution I used was at 1:3000, using a human brain as a control. Next time I might try Chemicon, as the other person on the Histonet mentioned. Good Luck Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Mike Bromley 2/24/2004 4:51:30 AM >>> Hi All Has anyone immunostained for substance P on formalin fixed paraffins. I would appreciate info re: antibody type and source, retrieval etc Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbowden <@t> ucsd.edu Tue Feb 24 12:19:48 2004 From: kbowden <@t> ucsd.edu (K. Bowden) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Re:(no subject) Villanueva References: <0F0551F2.71392D2B.0005167B@aol.com> Message-ID: <403B95C4.1050105@ucsd.edu> Myriam; You can buy Villanueva Osteochrome Bone Stain from Polysciences, Inc. When you buy it a protocol is provided with the stain. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-277-4970 CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Myri37@aol.com wrote: > hello > i'ev heard of villanueva stain, that is a good stain for bonea > do you have any protocol, how to prepare it ? or where could i buy it ? > thank you very much > Myriam > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cwscouten <@t> myneurolab.com Tue Feb 24 12:04:00 2004 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: [Histonet] Isopentane Message-ID: Isopentane can be chilled in a refrigeration device like the Clini RF, which also encloses it to prevent evaporation except when in use. It is great for quick freezing tissue, does not require the hazard of liquid nitrogen, and does not freeze so fast it cracks the block. See the following link for the Clini-RF http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=476401&catdesc=Histology+Equipment&CatThreeID=650&CatOneID=4&subcatdesc=Freezing+Devices&idsubcategory=187 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry Sent: Tuesday, February 24, 2004 11:24 AM To: Histonet Subject: Fwd: Re: Fwd: [Histonet] Isopentane Hello, I'm not aware of a procedure for quick freezing in isopentane alone, we quick freeze by immerging the sample in isopentane chilled by liquid nitrogen. Please will you share with me your protocol for quick freezing in isopentane. Thanks, Atoska >X-Mailer: Novell GroupWise Internet Agent 6.5.1 >Date: Tue, 24 Feb 2004 10:50:58 -0600 >From: "Nita Searcy" >To: >Subject: Re: Fwd: [Histonet] Isopentane > >Isopentane is used to "quick freeze" frozen sections- can't liquid >nitrogen be use in its place? No specialty work (muscles, etc.) but >routine frozens. > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > >>> > >Hello, I don't believe isopentane can be substituted for liquid >nitrogen, they're usually used in conjunction. However, dry ice can be >substituted for liquid nitrogen. Best wishes. Atoska > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > >From: "Nita Searcy" > >To: > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to > >histotechnology >and > > related fields > >List-Unsubscribe: > >, > > > > >> > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: >, > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] Isopentane > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no >version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > >Isopentane in the frozen lab due to volatility. Could not access > >Histologic article from May regarding laboratory accident. Anyone > >have any other incidents? Help would be appreciated. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Myri37 <@t> aol.com Tue Feb 24 11:59:42 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Villanueva stain Message-ID: <88.43cda7d.2d6ceb0e@aol.com> hello i'ev heard of villanueva stain, that is a good stain for bone do you have any protocol, how to prepare it ? or where could i buy it ? thank you very much Myriam From brett_connolly <@t> merck.com Tue Feb 24 12:43:10 2004 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] substance p Message-ID: Are you all talking about Substance P or Substance P receptor?? Just curious. Brett -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Tuesday, February 24, 2004 12:53 PM To: M.Bromley@dgri.scot.nhs.uk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] substance p Hello Mike, I have stained for Substance P using a company I had never used before; The Arnel Products Co. P. O. Box 20045/ New York, NY 10021 800-333-6078 I used it with DakoCytomation's ready to use Prot. K for 5 min. at room temp. The dilution I used was at 1:3000, using a human brain as a control. Next time I might try Chemicon, as the other person on the Histonet mentioned. Good Luck Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Mike Bromley 2/24/2004 4:51:30 AM >>> Hi All Has anyone immunostained for substance P on formalin fixed paraffins. I would appreciate info re: antibody type and source, retrieval etc Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From Sw918890 <@t> aol.com Tue Feb 24 13:03:31 2004 From: Sw918890 <@t> aol.com (Sw918890@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Re: GFP Antibody Message-ID: <19D29B11.44E7295E.0082A251@aol.com> -- We are in the process of working up the Anti-Green Fluorescent Protein (GFP) on mouse brains. First of all, what source would you recommend for this antibody? We would prefer to use a polyclonal antibody since we are doing the stain on frozen tissue. Any protocols/optimal methodology would be appreciated. On the rare case where we get the antibody to work, we are experiencing severe photo bleaching within seconds of looking at the section. We are currently using Vectashield for our mounting media. Any other alternatives? Would you recommend cutting the sections and staining them on the same day? Any other special handling of the tissue would also provide the insight that I am needing. Thank you in advance, Steven R. Westra University of Iowa Health Care From Jackie.O'Connor <@t> abbott.com Tue Feb 24 13:43:36 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: [Histonet] Isopentane Message-ID: Charles and others - Did I read the original email wrong? I thought the original author (I've lost track who it is - Atoska?) wanted to get rid of the isopentane and use LiN2 instead? "due to the volatility"?? Jackie O' "Charles Scouten" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/24/2004 12:04 PM To: "Atoska S. Gentry" , "Histonet" cc: Subject: RE: Re: Fwd: [Histonet] Isopentane Isopentane can be chilled in a refrigeration device like the Clini RF, which also encloses it to prevent evaporation except when in use. It is great for quick freezing tissue, does not require the hazard of liquid nitrogen, and does not freeze so fast it cracks the block. See the following link for the Clini-RF http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=476401&catdesc=Histology+Equipment&CatThreeID=650&CatOneID=4&subcatdesc=Freezing+Devices&idsubcategory=187 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry Sent: Tuesday, February 24, 2004 11:24 AM To: Histonet Subject: Fwd: Re: Fwd: [Histonet] Isopentane Hello, I'm not aware of a procedure for quick freezing in isopentane alone, we quick freeze by immerging the sample in isopentane chilled by liquid nitrogen. Please will you share with me your protocol for quick freezing in isopentane. Thanks, Atoska >X-Mailer: Novell GroupWise Internet Agent 6.5.1 >Date: Tue, 24 Feb 2004 10:50:58 -0600 >From: "Nita Searcy" >To: >Subject: Re: Fwd: [Histonet] Isopentane > >Isopentane is used to "quick freeze" frozen sections- can't liquid >nitrogen be use in its place? No specialty work (muscles, etc.) but >routine frozens. > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > >>> > >Hello, I don't believe isopentane can be substituted for liquid >nitrogen, they're usually used in conjunction. However, dry ice can be >substituted for liquid nitrogen. Best wishes. Atoska > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > >From: "Nita Searcy" > >To: > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to > >histotechnology >and > > related fields > >List-Unsubscribe: > >, > > > > >> > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: >, > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] Isopentane > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no >version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > >Isopentane in the frozen lab due to volatility. Could not access > >Histologic article from May regarding laboratory accident. Anyone > >have any other incidents? Help would be appreciated. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Feb 24 13:20:20 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Perforin Message-ID: I have never used them, but both USBiological and Novocastra have a perforin ab that is supose to work on ffpe tissue. Good luck, Juan -----Original Message----- From: Elaine Dooley [mailto:DOOLEEO@shands.ufl.edu] Sent: Mon 2/23/2004 3:43 PM To: Histonet@pathology.swmed.edu Cc: Subject: [Histonet] Perforin Dear histonetters, Does anyone out there have a antibody called Perforin that works well in formalin fixed paraffin embedded tissues? Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronnie_Houston <@t> bshsi.com Tue Feb 24 14:07:00 2004 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] 2004 Executive War College on Lab and Pathology Management Message-ID: <530361BF03351B4CAE5270A05D3037B5036730CC@bsrexms01.BSHSIR.COM> Anyone going to this meeting in New Orleans, April 27-29? There is a lot on Anatomic Pathology, and Molecular Diagnostics - see agenda at http://www.darkreport.com/ewc/agenda.pdf Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 (804) 287 7906 - fax ronnie_houston@bshsi.com ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From marilyn.johnson <@t> gov.ab.ca Tue Feb 24 15:27:26 2004 From: marilyn.johnson <@t> gov.ab.ca (marilyn.johnson@gov.ab.ca) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] CMC mounting media Message-ID: Hi Histonetters, I am looking for a "CMC" combined stain/mounting medium. Apparently, this soln. can be pretinted with acid fuchsin or aniline blue to the mounting medium. This soln. is required to demonstrate minute radulae. Any replies would be greatly appreciated. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada From Barry.R.Rittman <@t> uth.tmc.edu Tue Feb 24 16:05:30 2004 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] CMC mounting media Message-ID: <566FB0B522443D43AF02D2ADBE35A6F063597F@UTHEVS3.mail.uthouston.edu> Marilyn For my information, I hate to appear ignorant but are radulae the teeth of mollusks? If so do they have calcium or are they primarily chitinous? I checked on the CMC dye mountant and believe that the stain is an acid fast stain - a carbol fuchsin. There is an interesting site that mentions this at http://www.gladescropcare.com/prvsm.html Thanks Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of marilyn.johnson@gov.ab.ca Sent: Tuesday, February 24, 2004 3:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CMC mounting media Hi Histonetters, I am looking for a "CMC" combined stain/mounting medium. Apparently, this soln. can be pretinted with acid fuchsin or aniline blue to the mounting medium. This soln. is required to demonstrate minute radulae. Any replies would be greatly appreciated. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amarusk1 <@t> FAIRVIEW.ORG Tue Feb 24 16:22:11 2004 From: amarusk1 <@t> FAIRVIEW.ORG (ANN MARUSKA) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] antibody MCP-1 Message-ID: Hi Histonetters, Has anyone worked with the antibody MCP-1? I am hoping to use ths with a researcher to stain ovarian tumors. We will be testing it in both mouse and human tissue. Any experience or info you might have with it would be most appreciated. As always, thanks for all your help! Ann Ann Maruska Fairview-University Medical Center Mpls. MN 55454 amarusk1@fairview.org 612-273-9119 The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:ANN MARUSKA EMAIL;WORK;PREF;NGW:AMARUSK1@FAIRVIEW.ORG ORG:;LAB N:MARUSKA;ANN TEL;WORK:612-273-9119 END:VCARD From AnthonyH <@t> chw.edu.au Tue Feb 24 17:00:07 2004 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:36 2005 Subject: Fwd: [Histonet] Isopentane Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E13D@simba.kids> Barry, We do not have this problem. When we freeze specimens in liquid nitrogen we shake the specimen and chuck vigorously in the liquid nitrogen and no ice crystal artifact. My experience is that Liquid nitrogen is faster than isopentane/N2. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Barry R Rittman [mailto:Barry.R.Rittman@uth.tmc.edu] Sent: Wednesday, 25 February 2004 4:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: Re: Fwd: [Histonet] Isopentane The purpose of using isopentane cooled with liquid nitrogen is to dissipate heat quickly and thus prevent the formation of a vapor barrier around the tissues. When nitrogen is used alone, the vapor barrier that is formed significantly decreases the rate of cooling and therefore increases the possibility of ice crystal formation and tissue damage. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry Sent: Tuesday, February 24, 2004 11:24 AM To: Histonet Subject: Fwd: Re: Fwd: [Histonet] Isopentane Hello, I'm not aware of a procedure for quick freezing in isopentane alone, we quick freeze by immerging the sample in isopentane chilled by liquid nitrogen. Please will you share with me your protocol for quick freezing in isopentane. Thanks, Atoska >X-Mailer: Novell GroupWise Internet Agent 6.5.1 >Date: Tue, 24 Feb 2004 10:50:58 -0600 >From: "Nita Searcy" >To: >Subject: Re: Fwd: [Histonet] Isopentane > >Isopentane is used to "quick freeze" frozen sections- can't liquid >nitrogen be use in its place? No specialty work (muscles, etc.) but >routine frozens. > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > >>> > >Hello, I don't believe isopentane can be substituted for liquid >nitrogen, they're usually used in conjunction. However, dry ice can be >substituted >for liquid nitrogen. Best wishes. Atoska > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > >From: "Nita Searcy" > >To: > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to > >histotechnology >and > > related fields > >List-Unsubscribe: > >, > > > > >> > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: >, > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] Isopentane > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on >swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no >version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > >Isopentane in the frozen lab due to volatility. Could not access > >Histologic article from May regarding laboratory accident. Anyone > >have any other incidents? Help would be appreciated. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Atoska S. Gentry B.S., HT(ASCP) >Research Assistant III >Scott-Ritchey Research Center >College of Veterinary Medicine >Auburn University, AL 36849 >Phone# (334)844-5579 Fax# (334)844-5850 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Marysia33 <@t> aol.com Tue Feb 24 21:35:59 2004 From: Marysia33 <@t> aol.com (Marysia33@aol.com) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] Any job openings in Las Vegas area? Message-ID: <116.2f20b264.2d6d721f@aol.com> I would appreciate it if any one knows of any histo jobs avail in the Las Vegas area please let me know. Thanks! Marysia From carl.hobbs <@t> kcl.ac.uk Wed Feb 25 02:35:00 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:36 2005 Subject: [Histonet] re ki67 Message-ID: <001001c3fb7a$424cfef0$e8345c9f@Carlos> Excellent information from Cattoretti, as usual, here http://icg.cpmc.columbia.edu/cattoretti/Protocol/Mouse_IHC/Antibodies_for_mouse_IHC.html Note his warning of cross-reactivity with one of the Ab reagents . Generally , I would steer clear of PCNA as a marker for proliferation as it's half life is many days. Ki67( 1/2 -life around 30mins) and H3( mitotic cells only) are my antigens to target. From G.A.McHardy <@t> arh.grampian.scot.nhs.uk Wed Feb 25 02:40:53 2004 From: G.A.McHardy <@t> arh.grampian.scot.nhs.uk (G.A.McHardy@arh.grampian.scot.nhs.uk) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] RE: Histonet Digest, Vol 3, Issue 30 [Scanned By SOPHOS Anti-Viru s] Message-ID: We are trying to source a meter suitable for checking air levels of Xylene. Does anyone know of a UK supplier? Thanks Graham McHardy Path Dept ARI Aberdeen G.A.McHardy@arh.Grampian.scot.nhs.uk > -----Original Message----- > From: histonet-request@lists.utsouthwestern.edu > [SMTP:histonet-request@lists.utsouthwestern.edu] > Sent: 24 February 2004 18:00 > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 3, Issue 30 [Scanned By SOPHOS > Anti-Virus] > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Would Recruiter who had opening in Indianapolis, IN > (Dunn-Jena, Patsy A) > 2. Available Leitz 1400 Sledge Microtome (* B.I.C*) > 3. Using Poly-L-Lysine to coat slides (Coulter, Diane) > 4. RE: ki67 or pcna (anti-mouse) (C.M. van der Loos) > 5. Yale University - Opening (Ramona Tolliver) > 6. TRAP stain components (Wester, Martha) > 7. Perforin (Elaine Dooley) > 8. Perforin (Elaine Dooley) > 9. Yale University - Opening (Ramona Tolliver) > 10. CMC mounting media (marilyn.johnson@gov.ab.ca) > 11. unsubscribe please (Jeremy Browne) > 12. (no subject) (Linresearch@aol.com) > 13. CD21 (Linresearch@aol.com) > 14. cutting frozen sections of myocardium (Shaumik Adhya) > 15. filtering stains (what paper?) > (Nancy.Walker@sanofi-synthelabo.com) > 16. substance p (Mike Bromley) > 17. Isopentane (Nita Searcy) > 18. filtering stains (what paper?) > (Nancy.Walker@sanofi-synthelabo.com) > 19. (no subject) (Myri37@aol.com) > 20. RE: filtering stains (what paper?) (Gary Gill) > 21. RE: filtering stains (what paper?) (Gary Gill) > 22. Fwd: [Histonet] cutting frozen sections of myocardium > (Atoska S. Gentry) > 23. Re: substance p (John C. Dennis) > 24. Fwd: [Histonet] Isopentane (Atoska S. Gentry) > 25. please unsubscribe (Martha Ward) > 26. Fwd: Re: Fwd: [Histonet] Isopentane (Atoska S. Gentry) > 27. RE: Re: Fwd: [Histonet] Isopentane (Barry R Rittman) > 28. Re: substance p (Dana Settembre) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 23 Feb 2004 13:07:14 -0500 > From: "Dunn-Jena, Patsy A" > Subject: [Histonet] Would Recruiter who had opening in Indianapolis, > IN > To: "histol-histopathol@lg.ehu.es" > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > > Please contact me privately at padunnje@hotmail.com. I would appreciate > it. > > Patsy Dunn-Jena > padunnje@hotmail.com > > > > ------------------------------ > > Message: 2 > Date: Mon, 23 Feb 2004 18:19:11 +0000 > From: "* B.I.C*" > Subject: [Histonet] Available Leitz 1400 Sledge Microtome > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > Leitz 1400 Sledge Microtome in excellent condition. > > Looking for best offer. > > Mike MacDougall > (800)783-9424 Ext.114 > > _________________________________________________________________ > Dream of owning a home? Find out how in the First-time Home Buying Guide. > http://special.msn.com/home/firsthome.armx > > > > > ------------------------------ > > Message: 3 > Date: Mon, 23 Feb 2004 13:23:11 -0500 > From: "Coulter, Diane" > Subject: [Histonet] Using Poly-L-Lysine to coat slides > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > > > Content-Type: text/plain; charset="iso-8859-1" > > We're presently a commercially prepared, liquid Poly-L-Lysine as an > adhesive > for some of our slides. Is anyone using a dry form to make up their own > solution? If so, where are you purchasing from and what concentration do > you use? (We use these slides for special stains.) > > > Thanks in advance, > Diane > > Diane Coulter, RIH Histology > > > > > ------------------------------ > > Message: 4 > Date: Mon, 23 Feb 2004 19:37:26 +0100 > From: "C.M. van der Loos" > Subject: [Histonet] RE: ki67 or pcna (anti-mouse) > To: histonet@pathology.swmed.edu > Message-ID: <3958ab398a63.398a633958ab@amc.uva.nl> > Content-Type: text/plain; charset=iso-8859-1 > > Daniel, > During the Animal Research Kit workshop at NSH 2003 in Louisville last > October I used a wonderful Ki67 antibody named CDC47 from Neomarkers. It > worked wonderful on FFPE mouse intestinal tissue sections after EDTA9.0 > HIER with all workshop attendees! Because CDC47 is raised in mouse, you > need the ARKit for detection. > Hope this helps. > Chris vand er Loos, PhD > Dept. of Pathology > Academical Medical Center > Amsterdam - The Netherlands > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL > EBERHARD > Sent: Monday, February 23, 2004 3:23 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] ki67 or pcna (anti-mouse) > > Dear All, > sorry if this question has been ask before, > I?m searching for a ki67 or pcna (anti-mouse) antibody > which works on pFA fixed tissue. > Thanks for any hints. > Daniel. > (-)-(-) > --------------------------- \"/ --- > Dr. Daniel Eberhard =V= > > Developmental Biology > & Molecular Pathology > > Graduate Programe on Pattern Formation > > University of Bielefeld > D 33501 Bielefeld/Germany > > FAX: xx49(0)521-106-5654 > > > > > ------------------------------ > > Message: 5 > Date: Mon, 23 Feb 2004 13:47:49 -0500 > From: Ramona Tolliver > Subject: [Histonet] Yale University - Opening > To: histonet@lists.utsouthwestern.edu > Message-ID: <5.2.0.9.2.20040223134732.0122d248@email.med.yale.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Good afternoon, > > Yale University has a 2nd Shift Histology Supervisor Position available > from 3:00 -11:00 p.m. If you know of anyone who may be interested in a > great opportunity with advancement potential, please forward this > information on to them. > > The posting is located at : > http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=124471. > > Salary starts at 65K and is commensurate with experience. Relocation > assistance is available. > > Thank you for your time! > > Ramona Tolliver > > > Ramona E. Tolliver > Human Resource Manager > Yale University School of Medicine > Department of Pathology > 310 Cedar Street > New Haven, CT 06520-8023 > Telephone: (203) 785-6689 > Fax: (203) 785-7303 > > ------------------------------ > > Message: 6 > Date: Mon, 23 Feb 2004 16:19:47 -0500 > From: "Wester, Martha" > Subject: [Histonet] TRAP stain components > To: > Message-ID: > <83899F0EC7671543B305FB5694024DE6BAD2BE@medimmune4.medimmune.com> > Content-Type: text/plain; charset="iso-8859-1" > > I am gearing up to do some TRAP staining, over a period of time. Can > anyone tell me if it is possible to make up and store the various stock > solutions, how to store them and for what period of time they remain > usable? It would help me out a great deal not to have to devote 1-2 hrs > just before every incubation period to make up fresh stocks. Thanks for > your help! > > Martha S. Wester > Associate Scientist > MedImmune, Inc. > Gaithersburg, MD 20878 > (240) 632-4794 > > > > > ------------------------------ > > Message: 7 > Date: Mon, 23 Feb 2004 16:43:32 -0500 > From: "Elaine Dooley" > Subject: [Histonet] Perforin > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Dear histonetters, > > Does anyone out there have a antibody called Perforin that works well in > formalin fixed paraffin embedded tissues? > > > Elaine Dooley > Shands Teaching Hospital > Gainesville FL > > 352-265-0111 ext 72117 > > > > > ------------------------------ > > Message: 8 > Date: Mon, 23 Feb 2004 16:43:32 -0500 > From: "Elaine Dooley" > Subject: [Histonet] Perforin > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Dear histonetters, > > Does anyone out there have a antibody called Perforin that works well in > formalin fixed paraffin embedded tissues? > > > Elaine Dooley > Shands Teaching Hospital > Gainesville FL > > 352-265-0111 ext 72117 > > > > > ------------------------------ > > Message: 9 > Date: Mon, 23 Feb 2004 16:48:22 -0500 > From: Ramona Tolliver > Subject: [Histonet] Yale University - Opening > To: histonet@lists.utsouthwestern.edu > Message-ID: <6.0.1.1.2.20040223164739.01dca2b8@email.med.yale.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > I'm resending this due to trouble with the URL for the position. It can > also be reached by going to www.yale.edu/jobs and searching Pathology. > > Thanks, > > Ramona > > > ~~~~~~~~~~~ > > Good afternoon, > > Yale University has a 2nd Shift Histology Supervisor Position available > from 3:00 -11:00 p.m. If you know of anyone who may be interested in a > great opportunity with advancement potential, please forward this > information on to them. > > The posting is located at : > http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=151919. > > Salary starts at 65K and is commensurate with experience. Relocation > assistance is available. > > Thank you for your time! > > Ramona Tolliver > > > Ramona E. Tolliver > Human Resource Manager > Yale University School of Medicine > Department of Pathology > 310 Cedar Street > New Haven, CT 06520-8023 > Telephone: (203) 785-6689 > Fax: (203) 785-7303 > > ------------------------------ > > Message: 10 > Date: Mon, 23 Feb 2004 14:55:05 -0700 > From: marilyn.johnson@gov.ab.ca > Subject: [Histonet] CMC mounting media > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Hi Histonetters, > I am looking for a "CMC" combined stain/mounting medium. Apparently, this > soln. can be pretinted with acid fuchsin or aniline blue to the mounting > medium. This soln. is required to demonstrate minute radulae. > Any replies would be greatly appreciated. > Thanks in advance. > > Marilyn Johnson > Alberta Agriculture > Edmonton, Alberta, Canada > > > > > > > > > ------------------------------ > > Message: 11 > Date: Tue, 24 Feb 2004 12:46:18 +1300 > From: "Jeremy Browne" > Subject: [Histonet] unsubscribe please > To: > Message-ID: <006401c3fa67$3ba6d2b0$91c7d882@ssc.auckland.ac.nz> > Content-Type: text/plain; charset="us-ascii" > > 4th attempt!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy > Maronto > Sent: Monday, February 23, 2004 9:21 AM > To: histonet@lists.utsouthwestern.edu; Myri37@aol.com > Subject: Re: [Histonet] MMA > > Myriam, > I have used the MMA/butylmethacrylate/methylbenzoate/peg 400/ benzoyl > peroxide (BPO) combination to embed undecalcified bone and small stented > arteries. It is an easy combination to make up and use. I expect you > have three changes suggested in your procedure. Mix each about 4 hours > before use. It seems I have the best results with this. The > infiltration time will have to be worked out and if vacuum is used, it > should be limited to a couple of hours after each change of plastic. I > make prepolymerized containers to place the tissue on and add the > embedding plastic on. It will keep down the bubbles. When embedding, > the air in the embedding container with the liquid plastic will need to > be flushed out with C02 (easiest to use), you can also use gaseous > nitrogen. It takes one to two days for complete polymerization in the > freezer. > Things that can cause problems with polymerization: > Containers not flushed enough that have oxygen in them will not fully > polymerize. I place the flushed embedding containers in a bell jar, > flush jar with CO2 and seal. This goes in the freezer. > Humid conditions can cause poorly polymerization ---some hard--some > still liquid. Usually have to replace the embedding plastic, flush with > CO2 and try again. You can use drierite in the bottom to help this. > > After the blocks are hard, the plastic is trimmed a little to the block > size I want to use in the microtome. I wet cut 4 micron sections and > place them on a chrome-gelatin coated slide with a couple of drops of > 70% alcohol, on a slide warmer. The section flattens out, I put a piece > of plastic on it, gently roll with a small wall paper roller. when I > get them done I clamp them with c-clamps. It is good idea to have a > small piece of wood and extra glass slide on each end. It prevents > slides from breaking. They get placed in the oven overnight. The > clamps removed, plastic removed, they are ready to deplasticize with > xylene and ready for staining. You may want to deplasticize with other > agents such as 2-methoxyethyl acetate for immunostaining. Your > procedure may give you more info for immunostaining. I remember doing > Brdu with success, however, we would get a halo effect around the > possitive cells, and I could not find anyone that had a solution to > this. I did find th > at > certain antibody diluents and blockers were a key in the success of > this. > > I hope this helps. > Nancy > MPI Research > Mattawan, MI > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 12 > Date: Mon, 23 Feb 2004 20:50:44 EST > From: Linresearch@aol.com > Subject: [Histonet] (no subject) > To: histonet@pathology.swmed.edu > Message-ID: <1e9.19a5a825.2d6c07f4@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > I am still trying to find a CD21 that works on FFPE rat tissues. > I would appreciate any suggestions. > Lin > > > ------------------------------ > > Message: 13 > Date: Mon, 23 Feb 2004 20:52:24 EST > From: Linresearch@aol.com > Subject: [Histonet] CD21 > To: histonet@pathology.swmed.edu > Message-ID: <8a.4306d47.2d6c0858@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > I am still tryinmg to find a CD21 that works on FFPE rat tissues. > Any suggestions would be appreciated. > Lin > > > ------------------------------ > > Message: 14 > Date: Tue, 24 Feb 2004 10:16:44 +0000 > From: Shaumik Adhya > Subject: [Histonet] cutting frozen sections of myocardium > To: histonet@lists.utsouthwestern.edu > Message-ID: <1077617804.403b248cc5e70@www.webmail.ucl.ac.uk> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Histonetters, > > I'm a clinician trying to get to grips with histotechnology techniques for > > research. I'm having a lot of difficulty cutting frozen sections of rat > myocardium. I seem to be cutting sections with a lot of holes in them, > and > the fibres seem poorly arrayed. I'm cutting rat myocardium to practice > before > human myocardial biopsies. > > The rats are killed, the hearts harvested, without fixation. They're cut > into > small pieces before staining for 20 hours in senescence associated beta > galactosidase solution (essentially x-gal solution at pH 6). They are > then > moutned into OCT, frozen in isopentane and cut on a cryotome at -20 > centigrade. The slides are then fixed in 3% paraformaldehyde, wawshed in > distilled water, before Haemotxylin & Eosin staining. > > Any advice, tips or tricks will be much appreciated. > > Shaumik Adhya > > > > > > ------------------------------ > > Message: 15 > Date: Tue, 24 Feb 2004 12:46:29 +0100 > From: Nancy.Walker@sanofi-synthelabo.com > Subject: [Histonet] filtering stains (what paper?) > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=iso-8859-1 > > > What grade of filter paper is good for filtering hemalun? The paper we > recently bough(Whatman 113V) seems to retain too much (very slow filtering > with rapid loss of staining quality). > > How many times do you refilter stains before discarding? > > thanks again, > > Nancy Walker > Molecular Biology Scientist > > Sanofi-Synthelbo Research > B.P. 37 Lab?ge Innopole > 31676 LABEGE CEDEX FRANCE > > nancy.walker@sanofi-synthelabo.com > tel : (33)561004179 fax :(33)561004001 > > > > > > > ------------------------------ > > Message: 16 > Date: Tue, 24 Feb 2004 12:51:30 -0000 > From: Mike Bromley > Subject: [Histonet] substance p > To: "Histonet (E-mail)" > Message-ID: <7325D637DFE2D211928800902733A7F303B94A40@DGAMTBDCEMS> > Content-Type: text/plain > > Hi All > Has anyone immunostained for substance P on formalin fixed paraffins. I > would appreciate info re: antibody type and source, retrieval etc > > Best Wishes > > Mike Bromley > > Chief Biomedical Scientist > Pathology > Dumfries & Galloway Royal Infirmary > Scotland, UK > > > This e-mail and any files transmitted with it are private and intended > > solely for the use of the individual or entity to whom they are > addressed. > > If you have received this e-mail in error please return it to the > address > > it > > came from telling them it is not for you and then delete it from your > > system. > > > > > > > ------------------------------ > > Message: 17 > Date: Tue, 24 Feb 2004 06:51:28 -0600 > From: "Nita Searcy" > Subject: [Histonet] Isopentane > To: > Message-ID: <04Feb24.065158cst.119053@healthcare2.sw.org> > Content-Type: text/plain; charset=US-ASCII > > For Vinny- or anyone. I am trying to substitute liquid nitrogen for > Isopentane in the frozen lab due to volatility. Could not access > Histologic article from May regarding laboratory accident. Anyone > have any other incidents? Help would be appreciated. > > > > ------------------------------ > > Message: 18 > Date: Tue, 24 Feb 2004 14:23:50 +0100 > From: Nancy.Walker@sanofi-synthelabo.com > Subject: [Histonet] filtering stains (what paper?) > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=iso-8859-1 > > > ----- R?achemin? par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04 > 14:14 ----- > > > Nancy Walker > > Pour : > histonet@lists.utsouthwestern.edu > 24/02/04 12:46 cc : > > Objet : filtering stains > (what paper?)(Document link: Nancy Walker) > > > > > > What grade of filter paper is good for filtering hemalun? The paper we > recently bough(Whatman 113V) seems to retain too much (very slow filtering > with rapid loss of staining quality). > > How many times do you refilter stains before discarding? > > thanks again, > > Nancy Walker > Molecular Biology Scientist > > Sanofi-Synthelbo Research > B.P. 37 Lab?ge Innopole > 31676 LABEGE CEDEX FRANCE > > nancy.walker@sanofi-synthelabo.com > tel : (33)561004179 fax :(33)561004001 > > > > > > > > ------------------------------ > > Message: 19 > Date: Tue, 24 Feb 2004 08:37:17 -0500 > From: Myri37@aol.com > Subject: [Histonet] (no subject) > To: histonet@pathology.swmed.edu > Message-ID: <0F0551F2.71392D2B.0005167B@aol.com> > Content-Type: text/plain; charset=iso-8859-1 > > hello > i'ev heard of villanueva stain, that is a good stain for bonea > do you have any protocol, how to prepare it ? or where could i buy it ? > thank you very much > Myriam > > > > ------------------------------ > > Message: 20 > Date: Tue, 24 Feb 2004 08:45:00 -0500 > From: Gary Gill > Subject: RE: [Histonet] filtering stains (what paper?) > To: "'Nancy.Walker@sanofi-synthelabo.com'" > , > Histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Filtration per se does not cause "rapid loss of staining quality" -- > regardless of pore size. However, the "very slow filtering" you've > described suggests you're using Harris hematoxylin, which typically throws > a > surface precipitate when exposed to atmospheric oxygen. That precipitate > is > actually aluminum-hematein, which is the active complex responsible for > staining. It forms because oxygen continues to oxidize initially > unoxidized > hematoxylin, which forms so much more aluminum-hematein that it exceeds > its > solubility limit in water. Repeatedly removing this complex by filtering > over-and-over again causes the rapid loss of staining quality. > > Mixing three parts of Harris hematoxylin with 1 part ethylene glycol fixes > the problem. Aluminum-hematein is several times more soluble in 20% > ethylene glycol than it is in water. > > Gary Gill > > -----Original Message----- > From: Nancy.Walker@sanofi-synthelabo.com > [mailto:Nancy.Walker@sanofi-synthelabo.com] > Sent: Tuesday, February 24, 2004 8:24 AM > To: Histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] filtering stains (what paper?) > > > > ----- R?achemin? par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04 > 14:14 ----- > > > Nancy Walker > > Pour : > histonet@lists.utsouthwestern.edu > 24/02/04 12:46 cc : > > Objet : filtering stains > (what paper?)(Document link: Nancy Walker) > > > > > > What grade of filter paper is good for filtering hemalun? The paper we > recently bough(Whatman 113V) seems to retain too much (very slow filtering > with rapid loss of staining quality). > > How many times do you refilter stains before discarding? > > thanks again, > > Nancy Walker > Molecular Biology Scientist > > Sanofi-Synthelbo Research > B.P. 37 Lab?ge Innopole > 31676 LABEGE CEDEX FRANCE > > nancy.walker@sanofi-synthelabo.com > tel : (33)561004179 fax :(33)561004001 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 21 > Date: Tue, 24 Feb 2004 08:45:00 -0500 > From: Gary Gill > Subject: RE: [Histonet] filtering stains (what paper?) > To: "'Nancy.Walker@sanofi-synthelabo.com'" > , > Histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Filtration per se does not cause "rapid loss of staining quality" -- > regardless of pore size. However, the "very slow filtering" you've > described suggests you're using Harris hematoxylin, which typically throws > a > surface precipitate when exposed to atmospheric oxygen. That precipitate > is > actually aluminum-hematein, which is the active complex responsible for > staining. It forms because oxygen continues to oxidize initially > unoxidized > hematoxylin, which forms so much more aluminum-hematein that it exceeds > its > solubility limit in water. Repeatedly removing this complex by filtering > over-and-over again causes the rapid loss of staining quality. > > Mixing three parts of Harris hematoxylin with 1 part ethylene glycol fixes > the problem. Aluminum-hematein is several times more soluble in 20% > ethylene glycol than it is in water. > > Gary Gill > > -----Original Message----- > From: Nancy.Walker@sanofi-synthelabo.com > [mailto:Nancy.Walker@sanofi-synthelabo.com] > Sent: Tuesday, February 24, 2004 8:24 AM > To: Histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] filtering stains (what paper?) > > > > ----- R?achemin? par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04 > 14:14 ----- > > > Nancy Walker > > Pour : > histonet@lists.utsouthwestern.edu > 24/02/04 12:46 cc : > > Objet : filtering stains > (what paper?)(Document link: Nancy Walker) > > > > > > What grade of filter paper is good for filtering hemalun? The paper we > recently bough(Whatman 113V) seems to retain too much (very slow filtering > with rapid loss of staining quality). > > How many times do you refilter stains before discarding? > > thanks again, > > Nancy Walker > Molecular Biology Scientist > > Sanofi-Synthelbo Research > B.P. 37 Lab?ge Innopole > 31676 LABEGE CEDEX FRANCE > > nancy.walker@sanofi-synthelabo.com > tel : (33)561004179 fax :(33)561004001 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 22 > Date: Tue, 24 Feb 2004 10:10:18 -0600 > From: "Atoska S. Gentry" > Subject: Fwd: [Histonet] cutting frozen sections of myocardium > To: Histonet > Message-ID: > <6.0.1.1.0.20040224100656.025955a0@mailhost.vetmed.auburn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > Hello is it possible for you to fix overnight or for 24hrs and then > cryoprotect in sucrose/fixative or sucrose/PB before freezing for > cryosectioning? If you know that fixation won't destroy the enzyme you > might try this method. Best wishes. Atoska > > >Date: Tue, 24 Feb 2004 10:16:44 +0000 > >From: Shaumik Adhya > >To: histonet@lists.utsouthwestern.edu > >User-Agent: Internet Messaging Program (IMP) 3.2.1 > >X-Originating-IP: 128.40.253.73 > >X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, > > helpdesk@ucl.ac.uk for more information > >X-UCL-MailScanner: Found to be clean > >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to histotechnology > and > > related fields > >List-Unsubscribe: > >, > > > > > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: > , > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 05628b3a735271e1629bc35bb8abda07 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] cutting frozen sections of myocardium > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on > swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no > version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >Hi Histonetters, > > > >I'm a clinician trying to get to grips with histotechnology techniques > for > >research. I'm having a lot of difficulty cutting frozen sections of rat > >myocardium. I seem to be cutting sections with a lot of holes in them, > and > >the fibres seem poorly arrayed. I'm cutting rat myocardium to practice > >before > >human myocardial biopsies. > > > >The rats are killed, the hearts harvested, without fixation. They're cut > > >into > >small pieces before staining for 20 hours in senescence associated beta > >galactosidase solution (essentially x-gal solution at pH 6). They are > then > >moutned into OCT, frozen in isopentane and cut on a cryotome at -20 > >centigrade. The slides are then fixed in 3% paraformaldehyde, wawshed in > >distilled water, before Haemotxylin & Eosin staining. > > > >Any advice, tips or tricks will be much appreciated. > > > >Shaumik Adhya > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > > > ------------------------------ > > Message: 23 > Date: Tue, 24 Feb 2004 10:22:25 -0600 (CST) > From: "John C. Dennis" > Subject: Re: [Histonet] substance p > To: Mike Bromley > Cc: "Histonet \(E-mail\)" > Message-ID: > Content-Type: TEXT/PLAIN; charset=US-ASCII > > Mike > > I used a rabbit polyclonal from Chemicon. It worked well. > > John Carroll Dennis > Anatomy, Physiology, and Pharmacology > 109 Greene Hall > Auburn University, AL 36849 > > > On Tue, 24 Feb 2004, Mike Bromley wrote: > > > Hi All > > Has anyone immunostained for substance P on formalin fixed paraffins. I > > would appreciate info re: antibody type and source, retrieval etc > > > > Best Wishes > > > > Mike Bromley > > > > Chief Biomedical Scientist > > Pathology > > Dumfries & Galloway Royal Infirmary > > Scotland, UK > > > > > This e-mail and any files transmitted with it are private and intended > > > solely for the use of the individual or entity to whom they are > addressed. > > > If you have received this e-mail in error please return it to the > address > > > it > > > came from telling them it is not for you and then delete it from your > > > system. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 24 > Date: Tue, 24 Feb 2004 10:12:15 -0600 > From: "Atoska S. Gentry" > Subject: Fwd: [Histonet] Isopentane > To: Histonet > Message-ID: > <6.0.1.1.0.20040224101051.0259e070@mailhost.vetmed.auburn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > Hello, I don't believe isopentane can be substituted for liquid nitrogen, > they're usually used in conjunction. However, dry ice can be substituted > for liquid nitrogen. Best wishes. Atoska > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > >From: "Nita Searcy" > >To: > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.3 > >List-Id: For the exchange of information pertaining to histotechnology > and > > related fields > >List-Unsubscribe: > >, > > > > > >List-Archive: > >List-Post: > >List-Help: > > >List-Subscribe: > , > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: [Histonet] Isopentane > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on > swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no > version=2.63 > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > >X-SA-Exim-Scanned: Yes > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > >Isopentane in the frozen lab due to volatility. Could not access > >Histologic article from May regarding laboratory accident. Anyone > >have any other incidents? Help would be appreciated. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > > > ------------------------------ > > Message: 25 > Date: Tue, 24 Feb 2004 12:02:30 -0500 > From: "Martha Ward" > Subject: [Histonet] please unsubscribe > To: > Message-ID: > > <61135F0455D33347B5AAE209B903A304076A4DD5@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="us-ascii" > > I will out of the lab from 2/24/2004 to 3/8/2004 > Martha Ward > Wake Forest University Baptist Medical Center > > > ------------------------------ > > Message: 26 > Date: Tue, 24 Feb 2004 11:23:43 -0600 > From: "Atoska S. Gentry" > Subject: Fwd: Re: Fwd: [Histonet] Isopentane > To: Histonet > Message-ID: > <6.0.1.1.0.20040224112043.025921c0@mailhost.vetmed.auburn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > Hello, I'm not aware of a procedure for quick freezing in isopentane > alone, > we quick freeze by immerging the sample in isopentane chilled by liquid > nitrogen. Please will you share with me your protocol for quick freezing > in > isopentane. Thanks, Atoska > > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 10:50:58 -0600 > >From: "Nita Searcy" > >To: > >Subject: Re: Fwd: [Histonet] Isopentane > > > >Isopentane is used to "quick freeze" frozen sections- can't liquid > >nitrogen be use in its place? No specialty work (muscles, etc.) but > >routine frozens. > > > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > > >>> > > > >Hello, I don't believe isopentane can be substituted for liquid > >nitrogen, > >they're usually used in conjunction. However, dry ice can be > >substituted > >for liquid nitrogen. Best wishes. Atoska > > > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > > >From: "Nita Searcy" > > >To: > > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > > >X-BeenThere: histonet@lists.utsouthwestern.edu > > >X-Mailman-Version: 2.1.3 > > >List-Id: For the exchange of information pertaining to histotechnology > >and > > > related fields > > >List-Unsubscribe: > > >, > > > > > > > > >List-Archive: > > >List-Post: > > >List-Help: > > > > >List-Subscribe: > >, > > > > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > > >Subject: [Histonet] Isopentane > > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on > >swlx162.swmed.edu > > >X-Spam-Level: > > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no > >version=2.63 > > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > > >X-SA-Exim-Scanned: Yes > > > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > > >Isopentane in the frozen lab due to volatility. Could not access > > >Histologic article from May regarding laboratory accident. Anyone > > >have any other incidents? Help would be appreciated. > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Atoska S. Gentry B.S., HT(ASCP) > >Research Assistant III > >Scott-Ritchey Research Center > >College of Veterinary Medicine > >Auburn University, AL 36849 > >Phone# (334)844-5579 Fax# (334)844-5850 > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > > > ------------------------------ > > Message: 27 > Date: Tue, 24 Feb 2004 11:40:54 -0600 > From: "Barry R Rittman" > Subject: RE: Re: Fwd: [Histonet] Isopentane > To: > Message-ID: > <566FB0B522443D43AF02D2ADBE35A6F077FCA4@UTHEVS3.mail.uthouston.edu> > Content-Type: text/plain; charset="us-ascii" > > > The purpose of using isopentane cooled with liquid nitrogen is to > dissipate heat quickly and thus prevent the formation of a vapor barrier > around the tissues. When nitrogen is used alone, the vapor barrier that > is formed significantly decreases the rate of cooling and therefore > increases the possibility of ice crystal formation and tissue damage. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska > S. Gentry > Sent: Tuesday, February 24, 2004 11:24 AM > To: Histonet > Subject: Fwd: Re: Fwd: [Histonet] Isopentane > > > > Hello, I'm not aware of a procedure for quick freezing in isopentane > alone, > we quick freeze by immerging the sample in isopentane chilled by liquid > nitrogen. Please will you share with me your protocol for quick freezing > in > isopentane. Thanks, Atoska > > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > >Date: Tue, 24 Feb 2004 10:50:58 -0600 > >From: "Nita Searcy" > >To: > >Subject: Re: Fwd: [Histonet] Isopentane > > > >Isopentane is used to "quick freeze" frozen sections- can't liquid > >nitrogen be use in its place? No specialty work (muscles, etc.) but > >routine frozens. > > > > >>> "Atoska S. Gentry" 02/24/04 10:12AM > > >>> > > > >Hello, I don't believe isopentane can be substituted for liquid > >nitrogen, they're usually used in conjunction. However, dry ice can be > >substituted > >for liquid nitrogen. Best wishes. Atoska > > > > >X-Mailer: Novell GroupWise Internet Agent 6.5.1 > > >Date: Tue, 24 Feb 2004 06:51:28 -0600 > > >From: "Nita Searcy" > > >To: > > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b > > >X-BeenThere: histonet@lists.utsouthwestern.edu > > >X-Mailman-Version: 2.1.3 > > >List-Id: For the exchange of information pertaining to > > >histotechnology > >and > > > related fields > > >List-Unsubscribe: > > >, > > > > > > > >> > > >List-Archive: > > >List-Post: > > >List-Help: > > > > >List-Subscribe: > >, > > > > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8 > > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > > >Subject: [Histonet] Isopentane > > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on > >swlx162.swmed.edu > > >X-Spam-Level: > > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no > >version=2.63 > > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003) > > >X-SA-Exim-Scanned: Yes > > > > > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for > > >Isopentane in the frozen lab due to volatility. Could not access > > >Histologic article from May regarding laboratory accident. Anyone > > >have any other incidents? Help would be appreciated. > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Atoska S. Gentry B.S., HT(ASCP) > >Research Assistant III > >Scott-Ritchey Research Center > >College of Veterinary Medicine > >Auburn University, AL 36849 > >Phone# (334)844-5579 Fax# (334)844-5850 > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 28 > Date: Tue, 24 Feb 2004 12:52:45 -0500 > From: Dana Settembre > Subject: Re: [Histonet] substance p > To: M.Bromley@dgri.scot.nhs.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Hello Mike, > I have stained for Substance P using a company I had never used before; > The Arnel Products Co. > P. O. Box 20045/ New York, NY 10021 800-333-6078 > > I used it with DakoCytomation's ready to use Prot. K for 5 min. at room > temp. > The dilution I used was at 1:3000, using a human brain as a control. > > Next time I might try Chemicon, as the other person on the Histonet > mentioned. > > Good Luck > Dana Settembre > University Hospital - UMDNJ > Newark, NJ > > > >>> Mike Bromley 2/24/2004 4:51:30 AM >>> > Hi All > Has anyone immunostained for substance P on formalin fixed paraffins. > I > would appreciate info re: antibody type and source, retrieval etc > > Best Wishes > > Mike Bromley > > Chief Biomedical Scientist > Pathology > Dumfries & Galloway Royal Infirmary > Scotland, UK > > > This e-mail and any files transmitted with it are private and > intended > > solely for the use of the individual or entity to whom they are > addressed. > > If you have received this e-mail in error please return it to the > address > > it > > came from telling them it is not for you and then delete it from > your > > system. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 3, Issue 30 > *************************************** From carl.hobbs <@t> kcl.ac.uk Wed Feb 25 03:03:39 2004 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] re GFP AB Message-ID: <002701c3fb7e$425d55d0$e8345c9f@Carlos> I use Molecular Probe's rabbit poly A6455; superb on FFPW sections after TRIS pH10 M/W treatment. Around 1/700 for ABC- DAB and indirect fluorescence using ALEXA fluorochromes( pwax sections mounted in Mowiol containing DABCO and Azide to retard fading) From daniel.eberhard <@t> uni-bielefeld.de Wed Feb 25 04:50:44 2004 From: daniel.eberhard <@t> uni-bielefeld.de (DANIEL EBERHARD) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] GFP AB In-Reply-To: <002701c3fb7e$425d55d0$e8345c9f@Carlos> References: <002701c3fb7e$425d55d0$e8345c9f@Carlos> Message-ID: <403C7E04.4050104@UNI-BIELEFELD.DE> After trying several GFP AB?s, I use MBL?s rabbit polyclonal GFP -antibody (No 598). It works perfect on pFA fixed tissue in 1:100 to 1:500 dilutions without any special section treatments. Daniel >I use Molecular Probe's rabbit poly A6455; superb on FFPW sections after >TRIS pH10 M/W treatment. Around 1/700 for ABC- DAB and indirect fluorescence >using ALEXA fluorochromes( pwax sections mounted in Mowiol containing DABCO >and Azide to retard fading) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- (-)-(-) --------------------------- \"/ --- Dr. Daniel Eberhard =V= Developmental Biology & Molecular Pathology Graduate Programe on Pattern Formation University of Bielefeld D 33501 Bielefeld/Germany FAX: xx49(0)521-106-5654 (-)-(-) --------------------------- \"/ --- =V= From gudrun.lang <@t> aon.at Wed Feb 25 06:47:33 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] AChe Mb.Hirschsprung Message-ID: <005201c3fb9d$89db8c50$eeeea8c0@SERVER> A question about the performing of AChe on Rectumbiopsies: We have difficulties to purchase the isoOMPA (inhibitor of unspecific Cholinesterase). Can anyone tell me, what happens in the cuts of rectumbiopsies, when we omit the iso-OMPA? What other inhibitors work for the same purpose? (recipe?) Thanks in advance Gudrun Lang, Austria From ronald <@t> klinipath.nl Wed Feb 25 07:40:54 2004 From: ronald <@t> klinipath.nl (Ronald Kusters) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] dotting pens In-Reply-To: Message-ID: <002c01c3fba4$fed38830$3b01a8c0@server.klinipath.nl> Dear Histonetters, As specialised supplier for Pathology and Cytology products in Europe we also sell the Pilot Dotting pens in the 4 colours available. If you want to order these pens you can contact our US distributor Mercedes Medical in Sarasota Florida www.mercedesmedical.com I've checked with Pilot but in Europe these pens are NOT discontinued, only for the US for some strange reason but nevertheless. Please ask Scott Gerber for more information. Our Cat# is 4050-? Z for Black B for Blue R for Red G for Green The KLINIMARKER Pens are packed per 12 in a box. Hope this will help some of you. Kind regards, Klinipath bv Benelux Ronald Kusters The dotting pens our Pathologists and Cytotechs like are being discontinued by Pilot (Extra Fine Point Permanent Marker). We have tried the Ultra Fine PinPoint Pen from Eberhard Faber but without success. Does anyone have any recommendations? Thank you. Anna Inman SMH Pathology (970)244-7624 fax (970) 244-2100 ainman@stmarygj.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfail <@t> toolkitmail.com Wed Feb 25 08:36:40 2004 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Cresyl echt violet stain Message-ID: <403cb2f8.57.3c38.1171669674@toolkitmail.com> I would appreciate it if someone could help me out. I need the cresyl echt violet procedure from Frieda Carson's book. Thank you From Myri37 <@t> aol.com Wed Feb 25 02:58:32 2004 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] help Message-ID: <2254941A.1CD1D4FC.0005167B@aol.com> hello do you have any information about TRAP stain for osteoblasts, and ALP for osteoclasts Thanking you Myriam natural implant From Fulvio.Magara <@t> ibcm.unil.ch Wed Feb 25 10:24:34 2004 From: Fulvio.Magara <@t> ibcm.unil.ch (Fulvio Magara) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] purkinje cells Message-ID: <4.2.0.58.20040225162130.01748898@pop-server.unil.ch> HAllo, any suggestion for the best way to fix and cut a mouse cerebellum to evidentiate loss of Purkinje cells? ( I thought of paraformaldehyde fixation, dehydration and inclusion in paraffine, cutting parasagittal 8 microns, staining Cresylviolet or HE...) Fulvio Magara, Ph.D. Institut de Biologie Cellulaire et Morphologie Rue du Bugnon, 9 CH - 1005 Lausanne Tel. +41 +21 692 51 53 Office 692 52 55 FAX +78 860 65 78 Mobile From juan.gutierrez <@t> christushealth.org Wed Feb 25 10:52:57 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Adenovirus & RSV Message-ID: Is anyone running this two immunos? I need help getting them to work either on the Ventana or DAKO stainers. Also if somebody has a source for controls, I would appreciate an address. Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare San Antonio, TX 78207 (210)704-2533 From bwhitaker <@t> brownpathology.com Wed Feb 25 11:15:24 2004 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] non-gyn cytology specimens Message-ID: <000001c3fbc2$f50923f0$3601a8c0@brownpathology.net> Hi Everyone! As someone who has always preferred to avoid cytology specimens like the plague, I now find myself in the position of outfitting a lab from scratch that must deal with non-gyn cytologies in relatively low numbers. Can anyone offer their opinions and suggestions regarding ThinPrep vs Cytospins and anything else that may be out there that I am not aware of? Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 From Terry.Marshall <@t> rothgen.nhs.uk Wed Feb 25 11:22:19 2004 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] non-gyn cytology specimens Message-ID: I was an early user and supporter of cytospin. No longer. They have become expensive and cumbersome/safety oriented, without in any way addressing the problems of getting an even thin spread that is neither too wet or too dry when it emerges. If you get a good preparation you could cover it up with a blouse button. *Furthermore*, as Spriggs insisted long ago, it does nothing that can't be done easier in a conventional centrifuge with proper technique. Enter ThinPrep - ah, bliss. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: 25 February 2004 17:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-gyn cytology specimens Hi Everyone! As someone who has always preferred to avoid cytology specimens like the plague, I now find myself in the position of outfitting a lab from scratch that must deal with non-gyn cytologies in relatively low numbers. Can anyone offer their opinions and suggestions regarding ThinPrep vs Cytospins and anything else that may be out there that I am not aware of? Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fulvio.Magara <@t> ibcm.unil.ch Wed Feb 25 11:31:44 2004 From: Fulvio.Magara <@t> ibcm.unil.ch (Fulvio Magara) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] thanks for your answer _ Purkinje cells Message-ID: <4.2.0.58.20040225172929.0174fb70@pop-server.unil.ch> Hallo and goodbye, looks like the best is anti-calbindin immunostaining on paraffin sections as thin as 2 microns ( I will however try frozen 14 micron sections on half the brain). Thank yee everybody, F Magara From sa.drew <@t> hosp.wisc.edu Wed Feb 25 11:39:26 2004 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Adenovirus & RSV Message-ID: We're running NeoMarker's adenovirus on the Nexus/ES after HIER in citrate...1:200 dilution... -----Original Message----- From: GUTIERREZ, JUAN [mailto:juan.gutierrez@christushealth.org] Sent: Wednesday, February 25, 2004 10:53 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Adenovirus & RSV Is anyone running this two immunos? I need help getting them to work either on the Ventana or DAKO stainers. Also if somebody has a source for controls, I would appreciate an address. Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare San Antonio, TX 78207 (210)704-2533 From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Feb 25 12:05:50 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] thanks for your answer _ Purkinje cells Message-ID: Or Calretinin, perhaps -----Original Message----- From: Fulvio Magara [mailto:Fulvio.Magara@ibcm.unil.ch] Sent: 25 February 2004 17:32 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thanks for your answer _ Purkinje cells Hallo and goodbye, looks like the best is anti-calbindin immunostaining on paraffin sections as thin as 2 microns ( I will however try frozen 14 micron sections on half the brain). Thank yee everybody, F Magara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Feb 25 12:33:03 2004 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] non-gyn cytology specimens In-Reply-To: References: Message-ID: At 5:22 PM +0000 2/25/04, Marshall Terry Dr, Consultant Histopathologist wrote: >*Furthermore*, as Spriggs insisted long ago, it does nothing that >can't be done easier in a conventional centrifuge with proper >technique. > >Enter ThinPrep - ah, bliss. Agreed Terry, but for a small lab ThinPrep is too expensive unless one is also using it for PAPs. Even then it may be too expensive considering the cost of small numbers of kits. We do about 150-200 non PAP cytologies a year and use old fashioned, conventional techniques. Our major problem is not with our labs technical considerations, but getting appropriately fixed material from the hospitals and doctors offices. Cytec is now approaching smaller labs like mine and is giving an instrument ($54,000) for use for a 3 year contract for a given number of kits per year. The PAP kits cost more (I presume) at $10 per kit, but it still made economic sense for me. Besides, my Gyns were demanding it and I would have lost tissue accounts if I did not provide it. I have not yet determined yet if the non-gyn kits will make sense economically. -- _______________ Bill Blank, MD Heartland Lab, Inc From donna <@t> phxbio.com Wed Feb 25 12:40:52 2004 From: donna <@t> phxbio.com (Donna Brown) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] ISH reference books In-Reply-To: <4.2.0.58.20040225162130.01748898@pop-server.unil.ch> References: <4.2.0.58.20040225162130.01748898@pop-server.unil.ch> Message-ID: <6.0.1.1.0.20040225123719.027e19f8@phxbio.com> We're expanding our reference library and I'm hoping some of you involved with in situ hybridization will have a few recommendations on good lab manuals and general methods books for hybridization procedures and probe labeling. Thanks for your help in advance- Donna Donna Brown Phoenix BioTechnologies 1000 Meridian Street Huntsville, AL 35801 http://phxbio.com From asuarez <@t> mrl.ubc.ca Wed Feb 25 13:28:57 2004 From: asuarez <@t> mrl.ubc.ca (Agripina Suarez) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] ISH reference books Message-ID: Hi Donna, I find Roche Molecular Biochemicals' Nonradioactive In Situ Hybridzation Application Manual very, very useful if you're doing ISH. Agripina C. Suarez McDonald Research Laboratories iCAPTURE Centre, St Paul's Hospital 1081 Burrard St, Vancouver B.C., Canada V6Z 1Y6 Tel no. 604 682 2344 local 62703 Fax no. 604 806 8351 From jmitchell <@t> neurology.wisc.edu Wed Feb 25 13:29:24 2004 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean A.)) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Iowa/Minnesota/Wisconsin Tri-State Symposium Message-ID: <061D5F3CF2DFDF4EADF5611CC1218C4512A6D4@nrl-lorenz.neurology.wisc.edu> The histology societies of Iowa, Minnesota & Wisconsin will be hosting their first ever Tri-State Histology Symposium: April 28-30, 2004, Holiday Inn Five Flags - Dubuque, Iowa Registration Fee: $100 (includes Wed. night pizza party,lunches,"Murder Mystery Theme Banquet",workshops) Room Rates: $75.00/standard guest room Workshop & topic list include: The Chemistry of Processing & Staining - Jerry Fredenburgh, Preparation for the HT Examination - Bob Brunner, Antibodies 2004 - Dave Tacha, The Current State of Technology & the Advantages of Automating Special Stains, Microwave: The Whole Enchilada - Jan Minshew & Donna Willis, MonKEY Business: The KEY to Delegation - Jan Gardner & Judi Stasko, Design, Construction & Preliminary Validation of Tissue Midiarrays - David Hicks, For more information please contact one of the following organizers: Iowa: Judi Stasko, jstasko@nadc.ars.usda.gov Minnesota: Pam Hesch, hesch.pamela@mayo.edu Wisconsin: Jean Mitchell, jmitchell@neurology.wisc.edu From jcline <@t> wchsys.org Wed Feb 25 13:47:21 2004 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] fat fixation Message-ID: <001601c3fbd8$2fbe1540$1d2a14ac@wchsys.org> We are fixing our breast cases overnight in 10% buffered formalin, then placing the cassettes into Penfix, then process normally that night. I have a penfix station on the processor after two formalins. We are still having trouble with the breast tissue that is extremely fatty. ANY SUGGESTIONS PLEASE From Rcartun <@t> harthosp.org Wed Feb 25 13:56:40 2004 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Adenovirus & RSV Message-ID: Should everyone be doing every immunoperoxidase available? It is my opinion that if you don't do an immunoperoxidase stain on a regular basis, you shouldn't be doing it! I see more problems when labs attempt to do an IP stain when they don't get many requests for it. I realize that pressure may come from your pathologist(s), but you simply tell them that we don't get enough request for this IP stain to make it cost-effective, or that you don't have the technical expertise or positive control material to guarantee quality control. Richard Cartun Director, Immunopathology Hartford Hospital Hartford, CT 06001 >>> "GUTIERREZ, JUAN" 02/25/04 11:52AM >>> Is anyone running this two immunos? I need help getting them to work either on the Ventana or DAKO stainers. Also if somebody has a source for controls, I would appreciate an address. Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare San Antonio, TX 78207 (210)704-2533 From spoulos <@t> saa.ars.usda.gov Wed Feb 25 14:06:53 2004 From: spoulos <@t> saa.ars.usda.gov (Sylvia Poulos) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] fat fixation Message-ID: Hi Joyce, We routinely fix fatty tissues (brain and fat) in Bouin's and never have a problem. Not sure if Bouin's is considered too risky to have in a clinical setting, but it'd be my first suggestion. Good luck, Sylvia Sylvia P. Poulos USDA-ARS-Animal Physiology Research Unit Athens, GA 30605 706-583-8279 706-542-0399 (fax) From ploykasek <@t> phenopath.com Wed Feb 25 14:28:41 2004 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Slide writers Message-ID: I would be interested in any feedback on the various slide "writers" that are out there. I've talked with reps, but would like some user feedback. There was a posting on this topic last week, but I didn't see any replies. Thanks. Patti Loykasek Phenopath Laboratories Seattle, WA From mari.ann.mailhiot <@t> leica-microsystems.com Wed Feb 25 14:56:02 2004 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] fat fixation Message-ID: Joyce It sounds like your fixation is good, unless the specimens are too thick.. You didn't say whether you are using xylene or xylene substitute and the time you would keep them in solution during processing. Another thing with fatty breast is sometimes the wax has not infiltrated enough. Try placing the breast blocks back in paraffin for a couple of hours. They end up cutting very well once infiltrated with wax. Best of luck. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Joyce Cline" To: "Histonet" Sent by: cc: histonet-bounces@lists.utsouth Subject: [Histonet] fat fixation western.edu 02/25/2004 01:47 PM We are fixing our breast cases overnight in 10% buffered formalin, then placing the cassettes into Penfix, then process normally that night. I have a penfix station on the processor after two formalins. We are still having trouble with the breast tissue that is extremely fatty. ANY SUGGESTIONS PLEASE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From lizchlipala <@t> premierhistology.com Wed Feb 25 15:21:47 2004 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] fat fixation In-Reply-To: <001601c3fbd8$2fbe1540$1d2a14ac@wchsys.org> Message-ID: <000001c3fbe5$65711c20$74d48a80@LIZ> Joyce We used a technique that has been published in the Journal of Histotechnology 1999, volume 22 - by Dr Ruby. We would use this on lumpectomy and breast biopsy specimens. We would ink the entire specimens with marking ink and then slice thin sections through the entire breast biopsy placing each section on a folded paper towel in the order that it was sectioned. We would then roll up the paper towel with the breast slices within it, secure it with rubber bands and place it in alcoholic formalin overnight, we would then continue the next morning with grossing in of the specimen. The sections cut beautifully. I hope this helps. Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Wednesday, February 25, 2004 12:47 PM To: Histonet Subject: [Histonet] fat fixation We are fixing our breast cases overnight in 10% buffered formalin, then placing the cassettes into Penfix, then process normally that night. I have a penfix station on the processor after two formalins. We are still having trouble with the breast tissue that is extremely fatty. ANY SUGGESTIONS PLEASE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmalc <@t> unimelb.edu.au Wed Feb 25 16:35:39 2004 From: cmalc <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] endogenous peroxidases in liver sections Message-ID: <5.2.1.1.2.20040226092755.0275ac60@mail.staff.unimelb.edu.au> Hi, Has anyone got any good method for blocking endogenous peroxidases in mouse liver sections? We seem to have a lot of red and white blood cells and plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit (mouse on mouse and universal kits). Some pearls of wisdom will be very much appreciated. Thanks Cat From ABurnet <@t> chw.edu Wed Feb 25 14:54:50 2004 From: ABurnet <@t> chw.edu (Burnette, Andrew - MHA) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Slide writers Message-ID: <9342C1401745A147A1DD93AAEB29C24B01AFCDCA@aznv-msg-002.chw.edu> We've been using Sakura's slide writer for about 6 months now and have been fairly happy with it. The software, depending on your needs of course, is much better than earlier generations and with a few minor modifications, we could call it excellent for our needs. Mechanically, it has been reliable with a few "disconcerting" noises and the occasional slide misfeed. Sakura has been responsive to our concerns. I'll withhold comment on how responsive until I see upcoming software revisions, and until I hear some more definitive response to a few minor mechanical concerns. But in short, from what I've seen so far, I would buy the unit again. Hope that helps! Andy Burnette Section Head Anatomic Pathology St. Joseph's Hospital Phoenix AZ -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, February 25, 2004 1:29 PM To: histonet Subject: [Histonet] Slide writers I would be interested in any feedback on the various slide "writers" that are out there. I've talked with reps, but would like some user feedback. There was a posting on this topic last week, but I didn't see any replies. Thanks. Patti Loykasek Phenopath Laboratories Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KYAMAMOTO <@t> chromavision.com Wed Feb 25 16:48:52 2004 From: KYAMAMOTO <@t> chromavision.com (Yamamoto, Karen) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Job Opening Message-ID: > SUMMARY > ChromaVision is seeking a Quality Assurance / Medical Technologist. This > position requires extensive knowledge of science and laboratory processes, > specialized medical diagnostic laboratory tests, blood test procedures, > and medical terminology in order to perform medical laboratory diagnostic > tests. The successful candidate will be responsible for assisting in the > administration of the quality / regulatory department to ensure the > company is in compliance with all quality and regulatory requirements of > agencies governing the clinical laboratory industry including CAP, CLIA, > GLP, FDA, etc. To qualify, candidates must possess 3+ years of > administrative support experience ideally working in a clinical or > laboratory environment and possess strong working knowledge in the MS > Office suite specifically: Word, Excel, Outlook, and PowerPoint. A B.S. > degree, or equivalent, in physical or life sciences is required with a > minimum of 3 years of relevant experience in medical technology and / or > quality control in the in vitro diagnostic, medical device, or > pharmaceutical industry. Must be a California licensed Clinical Laboratory > Scientist (CLS). > Please submit cv to: ChromaVision Medical Systems 33171 Paseo Cerveza San Juan Capistrano, CA 92675 949.443.3355 attn: David Davis DDavis@chromavision.com > > From burdo <@t> salk.edu Wed Feb 25 17:20:01 2004 From: burdo <@t> salk.edu (Joe Burdo) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Fixation of frozen tissue? Message-ID: <000001c3fbf5$e7d59780$cec80a0a@JoesLaptop> All: I have some dry ice frozen human brain samples that I would like to section for IHC. We do not have a Cryostat in our lab, but we do have a freezing microtome. Can the frozen brain chunks be fixed in 4% PF, cryoprotected in 30% sucrose and then sectioned on the microtome for free floating IHC? I have never cut tissue that has been fixed after freezing, only tissue that has been fixed immediately after removal. Thanks, Joe ----------------------------- Joseph Burdo, Ph.D. Postdoctoral Associate Cellular Neurobiology Laboratory The Salk Institute for Biological Studies 10010 N. Torrey Pines Rd. La Jolla, CA, 92037 From pruegg <@t> colobio.com Wed Feb 25 18:27:05 2004 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] endogenous peroxidases in liver sections In-Reply-To: <5.2.1.1.2.20040226092755.0275ac60@mail.staff.unimelb.edu.au> Message-ID: Are you sure your staining is from endogenous peroxidase and not endogenous biotin? Are you using an avidin/biotin detection system? there is a lot of endogenous biotin in liver. try and non biotin detection system and see if the problem goes away. I have not been able to block biotin in mouse liver even with the most agressive and reported best a/b block reagents. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cathy Malcontenti-Wilson Sent: Wednesday, February 25, 2004 3:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] endogenous peroxidases in liver sections Hi, Has anyone got any good method for blocking endogenous peroxidases in mouse liver sections? We seem to have a lot of red and white blood cells and plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit (mouse on mouse and universal kits). Some pearls of wisdom will be very much appreciated. Thanks Cat _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From EJS1853393 <@t> aol.com Wed Feb 25 18:42:21 2004 From: EJS1853393 <@t> aol.com (EJS1853393@aol.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Please unsubscribe Message-ID: <69.41d3d947.2d6e9aed@aol.com> From burdo <@t> salk.edu Wed Feb 25 19:01:12 2004 From: burdo <@t> salk.edu (Joe Burdo) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Fixation of frozen tissue? In-Reply-To: <000001c3fbff$7fee6cf0$0b4dbad0@hppav> Message-ID: <000101c3fc04$0a8409c0$cec80a0a@JoesLaptop> Thanks George. I have fixed frozen sections before as well in another lab on a Cryostat, but have never done it at ambient room temp on a freezing microtome. Can the sections be taken off of the knife with a paintbrush and placed onto slides at room temp without destroying the tissue? All of my microtome experience is with free floating sections, i.e. taking the sections off of the knife and into wells of glycol for storage until staining, so getting the sections to lay out flat on a slide wasn't an issue. Thanks, Joe ----------------------------- Joseph Burdo, Ph.D. Postdoctoral Associate Cellular Neurobiology Laboratory The Salk Institute for Biological Studies 10010 N. Torrey Pines Rd. La Jolla, CA, 92037 -----Original Message----- From: George Cole [mailto:georgecole@ev1.net] Sent: Wednesday, February 25, 2004 4:29 PM To: 'Joe Burdo' Subject: RE: [Histonet] Fixation of frozen tissue? Joe; In the 6,359 muscles and 2000plus nerves, I always froze the tissues fresh and sectioned them. I did all fixation and processing on the fresh frozen sections. You can bolster your brain tissues that you want to cut by surrounding the tissues with OCT a little at a time, freezing it as you put it on, to build up a neat block with square edges on all 4 sides around the tissue. You can make sections then, putting the sections on slides as you go. The quality of the freezing job is unknown, but brain isn't as bad as muscle for suffering ice crystal artifact from poor freezing technique. But brain can get a little difficult to section sometimes. If the brain does look like it's full of holes, you can try thawing it, then freezing it again, with liquid nitrogen and isopentane---this worked with muscles. Fixation after freezing was successful in my lab about 120,000 times. The freezing job on the brain as it comes to you is the unknown here. All you can do is cross your fingers and try to go ahead. I hope it works all right. georgecole@ev1.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Burdo Sent: Wednesday, February 25, 2004 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation of frozen tissue? All: I have some dry ice frozen human brain samples that I would like to section for IHC. We do not have a Cryostat in our lab, but we do have a freezing microtome. Can the frozen brain chunks be fixed in 4% PF, cryoprotected in 30% sucrose and then sectioned on the microtome for free floating IHC? I have never cut tissue that has been fixed after freezing, only tissue that has been fixed immediately after removal. Thanks, Joe From SJones <@t> cvm.tamu.edu Wed Feb 25 23:17:46 2004 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Cresyl echt violet stain Message-ID: >>> rfail 2/25/2004 8:36:40 AM >>> I would appreciate it if someone could help me out. I need the cresyl echt violet procedure from Frieda Carson's book.Thank you There are two procedures from Carson's book, I've included them both here. I've also added a procedure I prefer from Bancroft's book. Sarah Cresyl Echt Violet Method for Nissl Substance Fixative: 10% neutral buffered formalin, cut paraffin sections at 6-8 microns Reagents Stock Cresyl Echt Violet Solution 0.5 gm Cresyl echt violet (C.I. 51010) 80 ml distilled water 20 ml absolute alcohol warm the distilled water, add the Cresyl echt violet, mix, and then add the alcohol. Working Cresyl Echt Violet Solution 45 ml Cresyl echt violet stock solution 15 drops glacial acetic acid Procedure 1. Deparaffinize and hydrate the sections to distilled water. 2. Stain sections in working Cresyl echt violet for 8 minutes. 3. Dehydrate sections with 95% and absolute alcohol, two changes each 4. Clear in two changes of xylene and mount in synthetic resin. Results: Nissl substance and nuclei-blue-purple, background-colorless Cresyl echt Violet from LFB procedure 0.1% Cresyl Echt Violet 0.1 gm Cresyl echt violet 100 ml distilled water Just before use, add 15 drops of 10% acetic acid solution, filter, and preheat. This solution is not very stable. Procedure 1. Deparaffinize sections and hydrate to distilled water. 2. Place in Cresyl echt violet solution for 6 minutes. Filter and preheat Cresyl echt violet solution to 57 degrees C. just before use. Keep hot during staining. 3. Differentiate in several changes of 95% alcohol. 4. Dehydrate in absolute alcohol, clear and mount. Results: Nissl substance and nuclei-violet Ref. Histotechnology, A Self-Instructional Text, by Freida Carson, pages 160, 161, 175 and now for something completely different...................... ;) I personally prefer this procedure from Theory and Practice of Histological Techniques, by John Bancroft and Alan Stevens, page 353 & 354 Cresyl Violet Acetate for Nissl Substance Fixation: Formalin fixed tissue Sectioning: Paraffin sections cut from 6-15 microns Solutions: 0.1% Cresyl Violet Stain 0.1 gm Cresyl Violet Acetate (Sigma Catalog No. C-5042) 100 ml Distilled water Dissolve the dye in water. Add 0.25 ml of Glacial Acetic Acid to each 100 ml of dye. This stain is stable for years. (until you get your tombstone shaped sticker from ASCP) Cresyl Violet Differentiator 44 ml 95% ethanol 60 ml chloroform 9 drops of Glacial Acetic Acid Technic: 1. Deparaffinize sections and hydrate to distilled water. 2. Stain in 0.1 % Cresyl Violet Stain for 5 minutes. 3. Wash in tap water. 4. Differentiate in Cresyl Violet Differentiator, dipping until color no longer runs off. This will take 5-10 dips. 5. Check differentiation under the microscope. (Nissl and nuclei only should be stained) 6. Dehydrate, clear and mount. Results: Nissl substance-blue Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From IKirbis <@t> onko-i.si Thu Feb 26 00:35:40 2004 From: IKirbis <@t> onko-i.si (Kirbis Srebotnik Irena) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] non-gyn cytology specimens Message-ID: it is not so difficult to prepare good slide from cytology samples, but it takes time to find the most suitable technique among variety of them. what kind of samples will you get?, FNAB samples or just different body fluids?, in our lab different approach/ techniques is used depending on sample - cytocentrifuge is great for low cellular /minimal volume samples, centrifuge for high volume samples, sometimes combination, for urines we use membrane filtration, there is nice and simple device available for membrane filtration http://www.emsdiasum.com/microscopy/products/histology/monoprep.aspx Irena Kirbis Dept. of cytopathology -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, February 25, 2004 6:22 PM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] non-gyn cytology specimens I was an early user and supporter of cytospin. No longer. They have become expensive and cumbersome/safety oriented, without in any way addressing the problems of getting an even thin spread that is neither too wet or too dry when it emerges. If you get a good preparation you could cover it up with a blouse button. *Furthermore*, as Spriggs insisted long ago, it does nothing that can't be done easier in a conventional centrifuge with proper technique. Enter ThinPrep - ah, bliss. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: 25 February 2004 17:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-gyn cytology specimens Hi Everyone! As someone who has always preferred to avoid cytology specimens like the plague, I now find myself in the position of outfitting a lab from scratch that must deal with non-gyn cytologies in relatively low numbers. Can anyone offer their opinions and suggestions regarding ThinPrep vs Cytospins and anything else that may be out there that I am not aware of? Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Walker <@t> sanofi-synthelabo.com Thu Feb 26 01:55:58 2004 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:37 2005 Subject: =?iso-8859-1?Q?R=E9f=2E_=3A_[Histonet]_Re=3A__GFP_Antibody?= Message-ID: Just wondering...do you have GFP mice??? in this case your brains should be fluorescent without any antibodies! yours truely, Nancy From kemlo <@t> tiscali.co.uk Thu Feb 26 03:02:45 2004 From: kemlo <@t> tiscali.co.uk (Kemlo Rogerson) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] non-gyn cytology specimens In-Reply-To: Message-ID: <002701c3fc47$507c5930$53062850@KEMLOS> ThinPrep? Only ThinPrep? You don't actually mean Liquid based Cytology do you? No? Only ThinPrep? Surely not.................. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 0208 970 8414 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts: kemlo@tiscali.co.uk or kemlo1@btinternet.com Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 25 February 2004 17:22 To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] non-gyn cytology specimens I was an early user and supporter of cytospin. No longer. They have become expensive and cumbersome/safety oriented, without in any way addressing the problems of getting an even thin spread that is neither too wet or too dry when it emerges. If you get a good preparation you could cover it up with a blouse button. *Furthermore*, as Spriggs insisted long ago, it does nothing that can't be done easier in a conventional centrifuge with proper technique. Enter ThinPrep - ah, bliss. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: 25 February 2004 17:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-gyn cytology specimens Hi Everyone! As someone who has always preferred to avoid cytology specimens like the plague, I now find myself in the position of outfitting a lab from scratch that must deal with non-gyn cytologies in relatively low numbers. Can anyone offer their opinions and suggestions regarding ThinPrep vs Cytospins and anything else that may be out there that I am not aware of? Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Thu Feb 26 05:44:46 2004 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] endogenous peroxidases in liver sections Message-ID: Following on from Patsy, we went on to using DAKO Envision detection system kit because of all the Biotin blocking that we were doing. And now things are clean. 3% Hydrogen Peroxide was a standard system for blocking endogenous peroxidase. Commercially available products are in the catalogues And I remember Methanolic peroxide years ago, where we had 4ml of 30% Hydrogen peroxide in 250ml of Methanol and blocked for thirty minutes! Which seems a little aggressive/desparate. Dave Manchester -----Original Message----- From: Cathy Malcontenti-Wilson [mailto:cmalc@unimelb.edu.au] Sent: 25 February 2004 22:36 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] endogenous peroxidases in liver sections Hi, Has anyone got any good method for blocking endogenous peroxidases in mouse liver sections? We seem to have a lot of red and white blood cells and plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit (mouse on mouse and universal kits). Some pearls of wisdom will be very much appreciated. Thanks Cat _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Thu Feb 26 08:02:49 2004 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] endogenous peroxidases in liver sections Message-ID: I work on a lot of mouse and have found the best thing is if you can insist on perfusion. Does not necessarily get rid of everything but makes background problems less troublesome. I usually us a 3% peroxide in buffer of choice or methanol. I do avoid BSA as a blocking agent as I find it is more problematic than helpful. I will frequently use no blocking and have found this to be just fine. If you are not using mouse on mouse a mouse adsorbed secondary is also helpful. No pearls or great wisdom but hopefully helpful! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Cathy Malcontenti-Wilson [mailto:cmalc@unimelb.edu.au] Sent: Wednesday, February 25, 2004 3:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] endogenous peroxidases in liver sections Hi, Has anyone got any good method for blocking endogenous peroxidases in mouse liver sections? We seem to have a lot of red and white blood cells and plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit (mouse on mouse and universal kits). Some pearls of wisdom will be very much appreciated. Thanks Cat _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RStaskiewicz <@t> agr.state.il.us Thu Feb 26 08:39:02 2004 From: RStaskiewicz <@t> agr.state.il.us (RStaskiewicz@agr.state.il.us) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Illinois Spring Meeting Message-ID: The Illinois Society for Histotechnologists announces the program for their annual Spring Meeting. It will be held May 13-14, 2004 at the Hilton Lisle/Naperville, which is just outside of Chicago. Speakers and topics include: Idiosyncrasies of Immunohistochemistry, Part One----------Ethel Macrea, Ventana Medical Products Understanding and Applying In Situ Hybridization in the Histology Lab-----Julie Saetelle, Dakocytomation MonKey Business-The Key to Delegation----Jan Gardner and Judi Stasko Microtomy: The Melding of Art and Science-----Jan Minshew, Leica Ht(ASCP) Examination Readiness Workshop----Roberta Smith Controlling Procedures------Dr. Freida Carson Double Immunostaing------Rebecca Orr, Biocare A Comprohensive Review of the Transimissible Spongiform Diseases: History, Pathology, Epidemiology and Safety---------------Dr. Richard French Laser Capture Microdissection, Specimen Preparation and Cell Acquisition--------Diane Sterchi History of Anatomy and Pathology-------------Dr. Thomas Haas and Sharon Schmetzer Mystery Theater II, "The Sequel" Troubleshooting Special Stains---------Joan Vesey, Richard-Allan Plus one morning of lectures, vendor exhibits and more!!! If you would like a program, please contact Dana Spears at dspears@agr.state.il.us or call at 309-344-2451 From mcauliff <@t> umdnj.edu Thu Feb 26 11:54:37 2004 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Fixation of frozen tissue? In-Reply-To: <000001c3fbf5$e7d59780$cec80a0a@JoesLaptop> References: <000001c3fbf5$e7d59780$cec80a0a@JoesLaptop> Message-ID: <403E32DD.1040803@umdnj.edu> Hi Joe: Since the brain is already frozen I think you should consider cutting it first, mounting the sections on slides, then fixing and staining. Since the brain was frozen without fixation the morphology won't be optimal no matter what you do. Also, there may be some diffusion of immunoractive things. On the other hand, you could thaw the tissue, especially if it is too big to fit on the stage of the microtome, fix, cryoprotect and then cut but I don't think your results will be any better, perhaps worse. If you do thaw the brain, I would do it in cold fix so the tissue gets fixed as it thaws. The other thing that occurs to me is to make a formalin/alcohol/acetic acid fix and chill that to below freezing, and put the tissue in that for 24 hours, then thaw. This last idea is pure speculation on my part! Geoff Joe Burdo wrote: >All: > > I have some dry ice frozen human brain samples that I would like to >section for IHC. We do not have a Cryostat in our lab, but we do have a >freezing microtome. Can the frozen brain chunks be fixed in 4% PF, >cryoprotected in 30% sucrose and then sectioned on the microtome for >free floating IHC? I have never cut tissue that has been fixed after >freezing, only tissue that has been fixed immediately after removal. > >Thanks, > >Joe > >----------------------------- >Joseph Burdo, Ph.D. >Postdoctoral Associate >Cellular Neurobiology Laboratory >The Salk Institute for Biological Studies >10010 N. Torrey Pines Rd. >La Jolla, CA, 92037 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From juan.gutierrez <@t> christushealth.org Thu Feb 26 09:39:10 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Adenovirus & RSV Message-ID: We are a non-profit private children's hospital that unfortunately does get a few cases of both. Right now we have a very sick kid and we can't start treatment for adeno without an accurate diagnosis(the treatment is very harsh on the patient). In general I agree with your statement, but then you get into the argument about how much is one life worth. Besides somebody has to run it so that everybody else can send their cases to them. Maybe we should have a database of all the tests that are available from the different labs that contact the histonet so that we know where we can send a specific test without having to waste time asking around and having to wait for replies. Just a thought. Juan -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Wed 2/25/2004 1:56 PM To: GUTIERREZ, JUAN; histonet@pathology.swmed.edu Cc: Subject: Re: [Histonet] Adenovirus & RSV Should everyone be doing every immunoperoxidase available? It is my opinion that if you don't do an immunoperoxidase stain on a regular basis, you shouldn't be doing it! I see more problems when labs attempt to do an IP stain when they don't get many requests for it. I realize that pressure may come from your pathologist(s), but you simply tell them that we don't get enough request for this IP stain to make it cost-effective, or that you don't have the technical expertise or positive control material to guarantee quality control. Richard Cartun Director, Immunopathology Hartford Hospital Hartford, CT 06001 >>> "GUTIERREZ, JUAN" 02/25/04 11:52AM >>> Is anyone running this two immunos? I need help getting them to work either on the Ventana or DAKO stainers. Also if somebody has a source for controls, I would appreciate an address. Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare San Antonio, TX 78207 (210)704-2533 From Doug.Geddes <@t> lhsc.on.ca Thu Feb 26 10:10:04 2004 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Leder's Method (re: Sodium barbiturate) Message-ID: Question for my technical specialist. Leder's method for neutrophilic myeloid and tissue mast cells for paraffin sections and blood films - Michaelis veronal acetate buffer requires sodium barbiturate. Sodium barbiturate can't be obtained in Canada or brought across the boarder. Any substitutions? Variations? Please help! Doug Geddes BSc. MLT London Health Sciences Centre London, Ontario, Canada From RFail <@t> Charleston.net Thu Feb 26 04:00:01 2004 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Cresyl echt violet stain In-Reply-To: Message-ID: <000401c3fc4f$4dc6b570$9f11a6a5@rena> Thank you Sarah -----Original Message----- From: Sarah Jones [mailto:SJones@cvm.tamu.edu] Sent: Thursday, February 26, 2004 12:18 AM To: histonet@lists.utsouthwestern.edu; rfail@TOOLKITMAIL.COM Subject: Re: [Histonet] Cresyl echt violet stain >>> rfail 2/25/2004 8:36:40 AM >>> I would appreciate it if someone could help me out. I need the cresyl echt violet procedure from Frieda Carson's book.Thank you There are two procedures from Carson's book, I've included them both here. I've also added a procedure I prefer from Bancroft's book. Sarah Cresyl Echt Violet Method for Nissl Substance Fixative: 10% neutral buffered formalin, cut paraffin sections at 6-8 microns Reagents Stock Cresyl Echt Violet Solution 0.5 gm Cresyl echt violet (C.I. 51010) 80 ml distilled water 20 ml absolute alcohol warm the distilled water, add the Cresyl echt violet, mix, and then add the alcohol. Working Cresyl Echt Violet Solution 45 ml Cresyl echt violet stock solution 15 drops glacial acetic acid Procedure 1. Deparaffinize and hydrate the sections to distilled water. 2. Stain sections in working Cresyl echt violet for 8 minutes. 3. Dehydrate sections with 95% and absolute alcohol, two changes each 4. Clear in two changes of xylene and mount in synthetic resin. Results: Nissl substance and nuclei-blue-purple, background-colorless Cresyl echt Violet from LFB procedure 0.1% Cresyl Echt Violet 0.1 gm Cresyl echt violet 100 ml distilled water Just before use, add 15 drops of 10% acetic acid solution, filter, and preheat. This solution is not very stable. Procedure 1. Deparaffinize sections and hydrate to distilled water. 2. Place in Cresyl echt violet solution for 6 minutes. Filter and preheat Cresyl echt violet solution to 57 degrees C. just before use. Keep hot during staining. 3. Differentiate in several changes of 95% alcohol. 4. Dehydrate in absolute alcohol, clear and mount. Results: Nissl substance and nuclei-violet Ref. Histotechnology, A Self-Instructional Text, by Freida Carson, pages 160, 161, 175 and now for something completely different...................... ;) I personally prefer this procedure from Theory and Practice of Histological Techniques, by John Bancroft and Alan Stevens, page 353 & 354 Cresyl Violet Acetate for Nissl Substance Fixation: Formalin fixed tissue Sectioning: Paraffin sections cut from 6-15 microns Solutions: 0.1% Cresyl Violet Stain 0.1 gm Cresyl Violet Acetate (Sigma Catalog No. C-5042) 100 ml Distilled water Dissolve the dye in water. Add 0.25 ml of Glacial Acetic Acid to each 100 ml of dye. This stain is stable for years. (until you get your tombstone shaped sticker from ASCP) Cresyl Violet Differentiator 44 ml 95% ethanol 60 ml chloroform 9 drops of Glacial Acetic Acid Technic: 1. Deparaffinize sections and hydrate to distilled water. 2. Stain in 0.1 % Cresyl Violet Stain for 5 minutes. 3. Wash in tap water. 4. Differentiate in Cresyl Violet Differentiator, dipping until color no longer runs off. This will take 5-10 dips. 5. Check differentiation under the microscope. (Nissl and nuclei only should be stained) 6. Dehydrate, clear and mount. Results: Nissl substance-blue Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From a-lisowski <@t> northwestern.edu Thu Feb 26 10:37:23 2004 From: a-lisowski <@t> northwestern.edu (a-lisowski@northwestern.edu) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Pricing on histo services??? Message-ID: <200402261637.i1QGboLT007589@hecky.it.northwestern.edu> I was wandering if many of you would contract out excess of the work. What is (or should be) the approximate pricing (in USA) per slide if the contact lab is given blocks (1) or samples (2) in an fixative to be trimmed, processed and sectioned. Any input would be appreciated thanks Andrew Lisowski Dir. Molecular Path Labs Northwestern University From Linke_Noelle <@t> Allergan.com Thu Feb 26 11:09:59 2004 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] working standards for techs in industry Message-ID: Hi everyone, This is a question for anyone in the research/pharma/biotech industry. What types of standards do you use for evaluating technicians for performance? For example, how fast should technicians have a study completed (trimming, cutting, etc)? We are looking for evaluation standards, but since we cannot be compared to hospital or reference lab standards, we are stuck! Any input would be greatly appreciated!! Thank you, Noelle Linke, BS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From powell_sa <@t> Mercer.EDU Thu Feb 26 12:35:41 2004 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] GSH meeting Message-ID: <002901c3fc97$5630dd10$e3f2acd1@powellsa1> The Georgia Society for Histotechnology 2004 annual meeting/symposium will be held at St. Simons Island, Georgia April 2-4, 2004. This is a combined meeting with the Georgia Society of Cytology the same as last year. It will be held at the Sea Palms Golf and Tennis Resort. To make immediate reservations call 1-800-841-6268 and contact me by email powell_sa@mercer.edu for the complete meeting registration form and hotel information. The deadline for the special hotel rate is March 2, which is next Tuesday, so call soon. Shirley Powell From ccdub <@t> earthlink.net Thu Feb 26 13:33:08 2004 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: I just love this list. It has helped me so much to do the best job I can. Now to my question: How do other labs prepare their prostate biopsies? I am talking about do you separate each biopsy into its' own cassette (whether they are received this way or not)? Or do you put everything that is received in one specimen bottle into one cassette (inking them)? We have a debate going on at our lab right now as to which way is best. I like to know when I am embedding that there will only be one biopsy in each cassette rather than having to look up the case to find out how many there are suppose to be and making sure I get them all in the mold and on the same plane. I have one pathologist who agrees with me. My co-workers and the PA want them one block per each container so there are less blocks to process and cut. Our other two pathologists say they don't have a preference. I would like to know what the consensus is on these two methods, or does anyone else have a better way of processing these things. Thank you in advance for your help in this matter, Cindy DuBois, HT ASCP DELTA PATHOLOGY ASSOC. STOCKTON, CA From Jackie.O'Connor <@t> abbott.com Thu Feb 26 14:05:11 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: When I worked in a hospital lab where we did multiple needle biopsy cases on a daily basis, the number of cores per vial (usually 4-6 as I recall) was documented by the surgeon, and the pathologist documented the same (hopefully). They were carefully aligned and laid flat wrapped in lens paper for processing. The number of cores was written on the cassette, so the persons embedding and cutting would know how many cores to find without looking at the paperwork. On a side note, only the very skilled technicians could embed and cut prostate cores - with multiple cores it was imperative that they all be on the same plane in the block - big difference embedding these opposed to hunks o' uterus. Microtomy was the same - if you were afraid to cut one - we didn't let you. We had a pretty good system going there. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Cindy DuBois Sent by: histonet-bounces@lists.utsouthwestern.edu 02/26/2004 01:33 PM To: Histonet cc: Subject: [Histonet] Prostate Needle Biopsies I just love this list. It has helped me so much to do the best job I can. Now to my question: How do other labs prepare their prostate biopsies? I am talking about do you separate each biopsy into its' own cassette (whether they are received this way or not)? Or do you put everything that is received in one specimen bottle into one cassette (inking them)? We have a debate going on at our lab right now as to which way is best. I like to know when I am embedding that there will only be one biopsy in each cassette rather than having to look up the case to find out how many there are suppose to be and making sure I get them all in the mold and on the same plane. I have one pathologist who agrees with me. My co-workers and the PA want them one block per each container so there are less blocks to process and cut. Our other two pathologists say they don't have a preference. I would like to know what the consensus is on these two methods, or does anyone else have a better way of processing these things. Thank you in advance for your help in this matter, Cindy DuBois, HT ASCP DELTA PATHOLOGY ASSOC. STOCKTON, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TrogadisJ <@t> smh.toronto.on.ca Thu Feb 26 15:59:45 2004 From: TrogadisJ <@t> smh.toronto.on.ca (Judy Trogadis) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] health and safety guidelines Message-ID: Dear Histonetters I have recently been asked to help implement and monitor stricter health and safety guidelines in cell/molecular biology research laboratories that have been, well, sort of casual about these matters in the past. Are there any published rules & regulations that most labs follow? I searched past Histonet archives and found one posting that mentioned "CAP guidelines". What are these and does anyone know any web sites where I could find more information? Thank you Judy Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 7Queen 30 Bond St. Toronto, ON M5B 1W8 Canada ph: 416-864-6060 x6337 pager: 416-685-9219 fax: 416-864-6043 trogadisj@smh.toronto.on.ca From mckeela <@t> ucmail.uc.edu Thu Feb 26 16:19:48 2004 From: mckeela <@t> ucmail.uc.edu (Lynn McKee) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Influenza B control slides Message-ID: <200402262213.ASE04884@mprelay.uc.edu> Does anyone know of a supplier for Influenza B control slides? We have exhausted our block and need to find something fast. Thanks in advance. Lynn McKee University of Cincinnati From gudrun.lang <@t> aon.at Thu Feb 26 16:22:46 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Re: Prostate Needle Biopsies Message-ID: <002701c3fcb7$0f72eab0$eeeea8c0@SERVER> We get 10 container per case (5 from the right side and 5 from the left side of prostata). We put them between sponges in the cassettes. If there are more than one biopsie in one container, we wright the number on the side of the cassette. Usually with all needle biopsies we don't embed more than two in one mold. We use sliding microtoms and have good results. Gudrun Lang, Austria A little thing besides: It's now 23:20 in Austria. So I will fall in my bed in the next minutes. I hope you have a nice day out there. > ----- Original Message ----- > From: "Cindy DuBois" > To: "Histonet" > Sent: Thursday, February 26, 2004 8:33 PM > Subject: [Histonet] Prostate Needle Biopsies > > > > I just love this list. It has helped me so much to do the best job I can. > > Now to my question: > > How do other labs prepare their prostate biopsies? I am talking about > > do you separate each biopsy into its' own cassette (whether they are > > received this way or not)? Or do you put everything that is received in > one > > specimen bottle into one cassette (inking them)? > > > > We have a debate going on at our lab right now as to which way is best. > > > > I like to know when I am embedding that there will only be one biopsy in > > each cassette rather than having to look up the case to find out how many > > there are suppose to be and making sure I get them all in the mold and on > > the same plane. I have one pathologist who agrees with me. > > > > My co-workers and the PA want them one block per each container so there > are > > less blocks to process and cut. > > > > Our other two pathologists say they don't have a preference. > > > > I would like to know what the consensus is on these two methods, or does > > anyone else have a better way of processing these things. > > > > Thank you in advance for your help in this matter, > > > > Cindy DuBois, HT ASCP > > DELTA PATHOLOGY ASSOC. > > STOCKTON, CA > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mckeela <@t> ucmail.uc.edu Thu Feb 26 16:30:18 2004 From: mckeela <@t> ucmail.uc.edu (Lynn McKee) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Kappa & Lambda by ISH Message-ID: <200402262224.ASE08541@mprelay.uc.edu> Has anyone had any luck running Kappa and Lambda by ISH on the Ventana Benchmarks? If so I would appreciate any information you can give me. Thanks. Lynn McKee University of Cincinnati From ernestinemiddleton <@t> yahoo.ca Thu Feb 26 18:24:28 2004 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Position opening Message-ID: <20040227002428.45090.qmail@web41605.mail.yahoo.com> Hello, I am posting this ad for a former Director of Pathology. HISTOLOGY SUPERVISOR. Excellent postion available at teaching hospital in Brooklyn, NY with four histotechnologists in CAP-approved laboratory. Competitive salary and benefits. Minimum 5 years experience, ASCP certification preferred. Please send CV to J. Li, MD, Fax: (718) 780-2740 or johnli@chpnet.org. You can alway send it to me and I will forward it to Dr. Li. Ernestine Middleton HT/HTL,MPA Lab. Manager Montefiore Med. Ct. Bronx, NY (718) 920-4157 Fax: (718) 547-1920 --------------------------------- Post your free ad now! Yahoo! Canada Personals From bhewlett <@t> cogeco.ca Thu Feb 26 19:30:33 2004 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Leder's Method (re: Sodium barbiturate) References: Message-ID: <004901c3fcd1$4b9d99d0$6400a8c0@bryanmainbox> Hi Doug, We always obtained our Na barbiturate directly from hospital pharmacy (they have a license!). However, you have at least two options. My first choice is; 1) Use The Napthol AS-D chloroacetate method of Li, C. Y., Lam, K.W. and Yam, , L.T. (1973). "Esterases in human leucocytes". J. Histochem. Cytochem., 21; 1-12 Briefly, it is as follows; Solution A 4% Pararosaniline in 2N HCl.................0.1mL 4% Aq. Sodium nitrite...........................0.1mL Mix and add to Sorensen's phosphate buffer, pH 6.3......35.0mL Solution B dissolve 20mg Napthol AS-D chloroacetate in 1.0 mL N,N-dimethylformamide. Mix solutions A & B, shake and filter directly into a Coplin jar. Method; a) sections to water b) Incubate in substrate at R.T. for 2 hours (Mast cells are faster to come up). c) rinse in water d) counterstain in Mayer's hemalum for 2 minutes. e) blue in Scott's 2 minutes. f) wash, DCM Easy-peasy!! Or you can; 2) Switch to Burstone's Napthol AS-D acetate/Fast blue RR technique, this uses 0.2M Tris buffer at about pH 6.8 (Must be below pH7.1). Problem is that you have to aqueous mount in glycerin jelly!!!!! Cheers my old China. Bryan ----- Original Message ----- From: "Doug Geddes" To: Sent: Thursday, February 26, 2004 11:10 AM Subject: [Histonet] Leder's Method (re: Sodium barbiturate) > Question for my technical specialist. > > Leder's method for neutrophilic myeloid and tissue mast cells for > paraffin sections and blood films - Michaelis veronal acetate buffer > requires sodium barbiturate. > > Sodium barbiturate can't be obtained in Canada or brought across the > boarder. > > Any substitutions? Variations? Please help! > > Doug Geddes BSc. MLT > London Health Sciences Centre > London, Ontario, Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> covad.net Thu Feb 26 19:16:11 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] control tissues References: Message-ID: <092101c3fcd4$3d065f80$ac544f44@domainnotset.invalid> Start with fresh, unfixed placenta or lung. - If lung, slightly edematous works best. - If placenta, press between towels several times, to try to get the excess RBCs out. Gross tissue into 2-3 mm cubes. Contact microbiology to let them know you are on your way (after previously talking with the supervisor days earlier about your needs). Have the microbiology prepare tubes of liquid incubating media (appropriate for the type(s) of micro-organism(s) you need). - Gram negative (E. coli works well) - Gram positive - AFB (non-pathogenic) - Fungus - Spirochetes (rarely is it ever syphilis. Usually some type of large spirochete, unfortunately. When large spirochetes are positive with the silver stains, the small syphilis are not being demonstrated yet.) (Helicobacter, as far as I know, cannot be grown in incubating media in routine microbiology labs.) Incubate tissue in culture media in 37 degree C. incubator overnight. Pour 10% NBF in tubes in morning, and allow to fix for 30-60 minutes. Pour out formalin/incubating media mixture, and pour in fresh NBF. Allow to fix all day. Place tissues in cassettes, label, process as usual. Embed. Write up cost containment report, on how you make X number of blocks of control tissues, which would equal Y number of slides. Which, if you had to buy them from an outside source would have cost you $Z amount of money. And the cost for you to do this procedures was a little bit of tech time, a few cassettes and some paraffin. Therefore, you just saved your facility lots of money! Document the collaboration between Histology and Microbiology. Everyone looks like a winner. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Evelyn Kaplan" To: "Therersa Stegall" Cc: "Histonet" Sent: Monday, February 09, 2004 10:49 PM Subject: RE: [Histonet] control tissues > Hi Theresa, > > Thanks for your reply but I am well familiar with the 'in-house' sytem of > control tissues. I am more interested in inocultaing tissues, such as fresh > placenta, with 'substances' to create a new control block. Just wondering if > anyone has tried it! > > Regards, > Evelyn > > -----Original Message----- > From: Therersa Stegall [mailto:STEGTM@samcstl.org] > Sent: Tuesday, February 10, 2004 12:00 AM > To: ekaplan@squ.edu.om > Subject: Re: [Histonet] control tissues > > > Evelyn: We make many of our own controls, using other cases that are > positive for the pathology tested for. Our pathologists keep us > informed on + Helicobacter Pylori, and for other things, we use tissue > raided from surgicals (such as appendix for Trichrome, non-neoplastic > spleen for reticulum, liver for PAS). Good liver for Fe controls is > hard to find, some Histonetters in England sent me a batch. I think you > could "raid" surgicals, and find nearly any tissue you need for > controls. Our computer system allows a natural language search, so it > gets easier. Peace, Terre > > >>> "Evelyn Kaplan" 2/8/2004 12:13:37 AM >>> > > > Good morning, > > I realise this is a subject which has been previously discussed but I > wonder > if anyone has had experience of 'manufacturing' their own control > blocks? I > have a student about to start a project and would apreciate any > protocols. > > Regards, > Evelyn Kaplan, > Dept of Pathology, > College of Medicine and Health Sciences, > Sultan Qaboos University, > Oman > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Tbarnhart <@t> primecare.org Fri Feb 27 06:51:46 2004 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] In-situ hybridization for HPV Message-ID: <1779904B5E82D511914C00D0B793339205BFD7E6@exchangent> I have been asked to develop in-situ testing for HPV on cervical biopsies. Our cytology lab already performs HPV testing on PAP smears. We have not, in the past, performed this or had our cervical biposies sent out to other labs for this testing. So, I'm curious as to how may labs perform this testing. I am also wondering, for labs who do this testing, do you perform in-situ HPV screening and then in-situ sub-typing testing or only a screening test? The procedures I have reviewed do not seem to be very standardized. I have seen procedures that mimic our immunohistochemical bench closely and some that are quite different. If you perform this testing, I would be interested in your protocols. Any and all input would be very much appreciated. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND tbarnhart@primecare.org Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From ian.montgomery <@t> bio.gla.ac.uk Fri Feb 27 07:32:31 2004 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice. Message-ID: <6.0.1.1.2.20040227132756.02d4bcb0@udcf.gla.ac.uk> Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From kbradshaw <@t> lcpath.com Fri Feb 27 08:31:28 2004 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Kappa & Lambda by ISH In-Reply-To: <200402262224.ASE08541@mprelay.uc.edu> Message-ID: Hi Everyone, We have had excellent results with Kappa and Lambda by ISH on the Benchmark. It's much cleaner and more definitive than IHC. If you have any technical questions feel free to contact me. Kari Bradshaw, HT(ASCP) Laboratory Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 (360)425-5620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lynn McKee Sent: Thursday, February 26, 2004 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa & Lambda by ISH Has anyone had any luck running Kappa and Lambda by ISH on the Ventana Benchmarks? If so I would appreciate any information you can give me. Thanks. Lynn McKee University of Cincinnati _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Feb 27 08:36:43 2004 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice. Message-ID: This would be a good time for the manufacturers to chime in. When I worked where we manufactured antibodies (not all for IHC) we followed strict freeze/thaw guidelines for antibodies. The maximum number of freeze thaw cycles for bulk antibodies was 3. After that, the potency of the antibodies deteriorated (per stability testing results). The potency of frozen as well as refrigerated antibodies was carefully monitored by pre-determined testing points to ensure reproducible testing results by customers. The expiration date of refrigerated antibodies and other reagents was determined when the potency started to drop off. The potency of antibodies was tested up to a month after the determined expiration date, so we could ensure customers would continue to get satisfactory results right up to the expiration date. Jackie O' Ian Montgomery Sent by: histonet-bounces@lists.utsouthwestern.edu 02/27/2004 07:32 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Advice. Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronnie_Houston <@t> bshsi.com Fri Feb 27 09:12:00 2004 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice. Message-ID: <530361BF03351B4CAE5270A05D3037B5036730E3@bsrexms01.BSHSIR.COM> I think the important factor though,is that antibodies are aliquoted into useable quantities to negate the freeze- thaw process affecting the antibody's stability. Never had any problems with any antibodies that have been frozen, including some >10 years old (before I get shot down, these antibodies were for research purposes!) Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 (804) 287 7906 - fax ronnie_houston@bshsi.com -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Friday, February 27, 2004 9:37 AM To: Ian Montgomery Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Advice. This would be a good time for the manufacturers to chime in. When I worked where we manufactured antibodies (not all for IHC) we followed strict freeze/thaw guidelines for antibodies. The maximum number of freeze thaw cycles for bulk antibodies was 3. After that, the potency of the antibodies deteriorated (per stability testing results). The potency of frozen as well as refrigerated antibodies was carefully monitored by pre-determined testing points to ensure reproducible testing results by customers. The expiration date of refrigerated antibodies and other reagents was determined when the potency started to drop off. The potency of antibodies was tested up to a month after the determined expiration date, so we could ensure customers would continue to get satisfactory results right up to the expiration date. Jackie O' Ian Montgomery Sent by: histonet-bounces@lists.utsouthwestern.edu 02/27/2004 07:32 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Advice. Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From ljb <@t> medicine.wisc.edu Fri Feb 27 09:53:33 2004 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:37 2005 Subject: Fwd: Re: [Histonet] Advice. Message-ID: >>> LaCinda Burchell 02/27/04 09:36AM >>> Dear Ian, We dilute our antibodies with glyceol, aliquote them, and then store them at -20F. That way they are kept very cold without the potential damage of ice crystal formation. Once an aliquote is thawed it is never put back into the freezer. So far this method has served very well. LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> Ian Montgomery 02/27/04 07:32AM >>> Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Fri Feb 27 11:03:05 2004 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: Thank God I don't have that problem anymore. When I did we use to: in one place I worked we put eosin in the processor to better visualize the cores and we put them all in one cassette; at another place we inked each bx a different color and submitted everything in one cassette also. The key to get them all in one plain was to use tissue thumpers when embedding. To make them fit into the small molds I groung the corners of the thumper with a Dremel tool, worked great. Can you tell we didn't want to have too many blocks? We use to get a bunch of them on Wednesdays so somebody always called in sick on Thursdays. Good luck. Juan -----Original Message----- From: Cindy DuBois [mailto:ccdub@earthlink.net] Sent: Thu 2/26/2004 1:33 PM To: Histonet Cc: Subject: [Histonet] Prostate Needle Biopsies I just love this list. It has helped me so much to do the best job I can. Now to my question: How do other labs prepare their prostate biopsies? I am talking about do you separate each biopsy into its' own cassette (whether they are received this way or not)? Or do you put everything that is received in one specimen bottle into one cassette (inking them)? We have a debate going on at our lab right now as to which way is best. I like to know when I am embedding that there will only be one biopsy in each cassette rather than having to look up the case to find out how many there are suppose to be and making sure I get them all in the mold and on the same plane. I have one pathologist who agrees with me. My co-workers and the PA want them one block per each container so there are less blocks to process and cut. Our other two pathologists say they don't have a preference. I would like to know what the consensus is on these two methods, or does anyone else have a better way of processing these things. Thank you in advance for your help in this matter, Cindy DuBois, HT ASCP DELTA PATHOLOGY ASSOC. STOCKTON, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rschoon <@t> email.unc.edu Fri Feb 27 11:05:02 2004 From: rschoon <@t> email.unc.edu (rschoon@email.unc.edu) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice on antibody storage In-Reply-To: <6.0.1.1.2.20040227132756.02d4bcb0@udcf.gla.ac.uk> References: <6.0.1.1.2.20040227132756.02d4bcb0@udcf.gla.ac.uk> Message-ID: <1077901502.403f78be83dfd@webmail8.isis.unc.edu> Ian, As I work in a research setting there are some antibodies that I may only use every 6 to 12 months. I make an estimate of how many slides I may have to do IHC on per run and then freeze aliquots of the antibody at -80 oC to do one run. To date (knock on wood), this has not failed me yet and some of these antibodies are over 10 years old and I'm getting comparable results to 10 years ago. Sometimes better as my methods have changed. Once they have been thawed I find that remain usable for about a month or so. I allways use them within a month but it could be that they might last a bit longer. Time to go as the University is closed due to weather and I need to shovel some snow.... Regards, Bob Robert Schoonhoven Quoting Ian Montgomery : > Antibody, store at 4C, do not freeze, stable for 1 year. > What's > the current thinking. Are these antibodies affected by freezing? Or > is it > just the company covering themselves against users thaw/freezing. In > the > past I've just aliquoted and frozen anyway. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > Graham Kerr Building, > Institute of Biomedical & Life Sciences, > University of Glasgow, > Glasgow, > G12 8QQ. > Tel: 0141 339 8855 > Office: 4652 > Lab: 6644. > Pager: 07625 702883 > e-mail: ian.montgomery@bio.gla.ac.uk > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tpmorken <@t> labvision.com Fri Feb 27 11:33:12 2004 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice. Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA217734A@usca0082k08.labvision.apogent.com> Ian, The vast majority of antibodies can be frozen, but I think you are on the right track in thinking the antibody manufacturer is trying to avoid problems with freeze/thaw of the vial they sell. If you aliquot, you will avoid that problem. However, if sodium azide is added, the refrigerator shelf life can be years. Whether to refigerate or freeze depends on how fast you will use the antibody up. Even if bacteria or mold grows it can often be filtered out and the antibody performs fine. For the most part, serum and ascites can be aliquoted and frozen as-is (they are well buffered and have loads of protein). Hybridoma supernatent requires addition of buffer, but that is the manufacturers job. Purified antibody is generally ok to freeze if at a concentration above 1mg/ml, but usually that is the concentration a manufacturer works from. A user will have a much lower concentration and so needs addition of a protein, such as 1% BSA to prevent adsorbtion to the vial surface. Most of the time that is added by the manufacturer. If you buy an antibody without azide or BSA you will have to consider that when storing the antibody. Precipitates in frozen or sometimes even refrigerated antibodies are usually cryoproteins that clump up. Filtering removes them but leaves the antibody. In rare cases an antibody is a cryoprotein and will precipitate. In those cases you cannot even refrigerate the antibody. Glycerol does not "preserve" the antibody the same way azide does (prevent organism growth), but stabilizes it in solution, as does BSA. Glycerol is nice to use for freezer aliquoting, however, because it stays liquid and you don't have to wait for it to thaw. It may sound complicated, but in over 20 years I have only had a handful of antibodies go bad from storage. In general if the antibody will be used up within two years, then make sure it has azide and just keep it in the fridge. If you want to keep it longer, aliquot and freeze. -20 is fine, but -80 is better for really long term storage. Tim Morken Lab Vision / Neomarkers www.labvision.com -----Original Message----- From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk] Sent: Friday, February 27, 2004 5:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Advice. Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histolog <@t> fcv.unl.edu.ar Fri Feb 27 11:55:26 2004 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice.....secondary-biotinylated??? In-Reply-To: <530361BF03351B4CAE5270A05D3037B5036730E3@bsrexms01.BSHSIR.COM> Message-ID: <000001c3fd5a$e1d93ba0$5a0cd2aa@unl.edu.ar> And the concentrated secondary-biotinylated antibodies?? -----Mensaje original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] En nombre de Houston, Ronnie Enviado el: Viernes, 27 de Febrero de 2004 12:12 p.m. Para: 'Jackie.O'Connor@abbott.com'; Ian Montgomery CC: histonet@lists.utsouthwestern.edu Asunto: RE: [Histonet] Advice. I think the important factor though,is that antibodies are aliquoted into useable quantities to negate the freeze- thaw process affecting the antibody's stability. Never had any problems with any antibodies that have been frozen, including some >10 years old (before I get shot down, these antibodies were for research purposes!) Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 (804) 287 7906 - fax ronnie_houston@bshsi.com -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Friday, February 27, 2004 9:37 AM To: Ian Montgomery Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Advice. This would be a good time for the manufacturers to chime in. When I worked where we manufactured antibodies (not all for IHC) we followed strict freeze/thaw guidelines for antibodies. The maximum number of freeze thaw cycles for bulk antibodies was 3. After that, the potency of the antibodies deteriorated (per stability testing results). The potency of frozen as well as refrigerated antibodies was carefully monitored by pre-determined testing points to ensure reproducible testing results by customers. The expiration date of refrigerated antibodies and other reagents was determined when the potency started to drop off. The potency of antibodies was tested up to a month after the determined expiration date, so we could ensure customers would continue to get satisfactory results right up to the expiration date. Jackie O' Ian Montgomery Sent by: histonet-bounces@lists.utsouthwestern.edu 02/27/2004 07:32 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Advice. Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ ________________________________________________________ ________________________________________________________________________ ________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihc <@t> unipathllc.com Fri Feb 27 12:10:11 2004 From: ihc <@t> unipathllc.com (IHC@unipathllc.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] C1q and Properdin Message-ID: Does anyone know of a source for unconjugated C1q and Properdin abs for use in FFPE? Thanks, Brianna Jackson, BS, QIHC UniPath, LLC Denver, CO 303-512-2220 ihc@unipathllc.com From Kathy.Johnston <@t> CLS.ab.ca Fri Feb 27 12:20:05 2004 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Acid Fuchsin stain for Ischemic Neurons Message-ID: <30C050525B881C4AAFF41E6D16543E68015D0362@mail3.cls.ab.ca> One of our residents is looking for this particular stain. We have found quite a few references to it in journals and on the Internet, but no recipe. Does anyone in Histoland do this particular stain, and if they do, would they be so kind as to forward their recipe! Thanks very much in advance! Kathy Johnston Tech II - Special Stains Anatomic Pathology - FMC Calgary Laboratory Services 1403-29 Street NW Calgary AB, Canada T2N 2T9 403-944-4760 403-290-4093 fax kathy.johnston@cls.ab.ca From ameeker <@t> mail.jhmi.edu Fri Feb 27 13:04:50 2004 From: ameeker <@t> mail.jhmi.edu (ALAN MEEKER) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: <3d67ee3db686.3db6863d67ee@jhmimail.jhmi.edu> Regarding the treatment of prostate needle biopsies, a paper recently appeared in the journal UROLOGY addressing this question. Here is the reference: Individual submission and embedding of prostate biopsies decreases rates of equivocal pathology reports. Chakshu Gupta, Jian Z. Rena and Kirk J. Wojnoa Urology Vol. 63, No.1, January 2004, pp. 83-86 This is the Conclusion from the abstract: "Multiple needle biopsies submitted in 1 to 2 containers tend to entangle and fragment and are difficult to embed in a single plane during processing. The resulting loss of tissue surface area makes a definitive diagnosis difficult on small foci of atypical glands, resulting in equivocal pathology reports. The results of our study indicate that individual submission and processing of prostate biopsies in 6 to 12 container kits reduces the monthly rates of equivocal diagnoses." -Alan Meeker From Gillian.Barlow <@t> cshs.org Fri Feb 27 13:19:11 2004 From: Gillian.Barlow <@t> cshs.org (Barlow, Gillian) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Antibody to m-cadherin? Message-ID: <3CFAA0108952D111A5BF00805FA6FB0F0794745C@PEDSNTAS.csmc.edu> Dear all Can anybody recommend a good antibody to m-cadherin for immunohistochemistry? We want to compare with n- and e-cadherin in sections of developing mouse embryos Many thanks! Gillian Gillian M. Barlow, PhD Postdoctoral Fellow Laboratory of Julie Korenberg, PhD, MD Cedars-Sinai Medical Center Davis Bldg, Lab 2007 110 George Burns Rd Los Angeles, CA 90048 Phone: (310) 423 7650 Fax: (310) 423 0302 From info <@t> instrumedics.com Fri Feb 27 14:16:14 2004 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Re: Histonet Digest, Vol 3, Issue 35 References: Message-ID: <018101c3fd6e$8d0bb350$6501a8c0@INSTRUMEDICS22> Anyone have a recommendation for the best paraffin for embedding hard bone. Any suggestions would be appreciated. Bernice schiller@instrumedics.com ----- Original Message ----- From: To: Sent: Friday, February 27, 2004 1:00 PM Subject: Histonet Digest, Vol 3, Issue 35 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > ---------------------------------------------------------------------------- ---- > Today's Topics: > > 1. GSH meeting (Shirley Powell) > 2. Prostate Needle Biopsies (Cindy DuBois) > 3. Re: Prostate Needle Biopsies (Jackie.O'Connor@abbott.com) > 4. health and safety guidelines (Judy Trogadis) > 5. Influenza B control slides (Lynn McKee) > 6. Re: Prostate Needle Biopsies (Gudrun Lang) > 7. Kappa & Lambda by ISH (Lynn McKee) > 8. Position opening (Ernestine Middleton) > 9. Re: Leder's Method (re: Sodium barbiturate) (Bryan Hewlett) > 10. Re: control tissues (Lee & Peggy Wenk) > 11. In-situ hybridization for HPV (Barnhart, Tammy) > 12. Advice. (Ian Montgomery) > 13. RE: Kappa & Lambda by ISH (Kari Bradshaw) > 14. Re: Advice. (Jackie.O'Connor@abbott.com) > 15. RE: Advice. (Houston, Ronnie) > 16. Fwd: Re: [Histonet] Advice. (LaCinda Burchell) > 17. RE: Prostate Needle Biopsies (GUTIERREZ, JUAN) > 18. Advice on antibody storage (rschoon@email.unc.edu) > 19. RE: Advice. (Morken, Tim - Labvision) > 20. RE: Advice.....secondary-biotinylated??? (Lab Histologia) > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> covad.net Fri Feb 27 19:08:02 2004 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] staining of anterior pituitary? References: <4030F349.12204.188E63A@localhost> <4032BB55.3000308@umdnj.edu> Message-ID: <01b701c3fd97$50e85bc0$792df944@domainnotset.invalid> I don't see any replies, so I'll take a stab at this. I think most people are doing IHC. If not within their own lab, then they are sending it out. Most labs don't get very many pituitary tumors, so buying the primary antibodies is very expensive for most labs that will do a pituitary IHC series once every X years. The problem with these other histology special stains is two-fold, in my opinion: 1. NOT TOO SPECIFIC: The best one can determine is if a particular tumor cell is acidophilic (staining with orange G, acid fuchsin, eosin, etc.) or basophilic (staining with Schiff, blue dyes of trichromes, etc.). Acidophil pituitary cells produce either growth hormones or prolactin producing cells. Basophil pituitary cells produce thyroid stimulating hormone, adrenal cortical stimulating hormones, or the gonadotrophins (follicle stimulating hormones, leutinizing hormone). So the best these beautiful histology stains could do it put the cells into a category. So if the tumor staining positive with acid fucsin and orange G, then it must be an acidophil tumor. But WHICH acidophil cell tumor - the cell producing growth hormone, or the cell producing prolactin hormone? The histology stains could not further differentiate than acidophil or basophil. So how did the pathologists determine this differentiation? Well - what were the symptoms of the patient? Was the patient growing taller (so must be a growth hormone tumor) or were their breasts lactating (prolactin tumor)? 2. CHROMOPHOBES: In any normal pituitary adenohypophysis (glandular portion, not the neurohypophysis part), about 10% of the cells are basophils, about 40% are acidophils, and about 50% are chromophobes - in other words, they don't stain with histology special stains. These chromophobes are any of the acidophils or basophils, but are simply not staining at that time. Possibly they are "immature" with only a few chemicals/hormones, or possibly they have recently released most of their chemicals/hormones, or possibly they are waiting in reserve so don't have a lot of these chemicals/hormones. For whatever the reason(s), these chromophobe cells do not have a lot of granules, so don't stain well with histology special stains (or not at all). And often, cancer tumor cells are so busy multiplying, they "forget" to make the granules and hormones, so as a result, in my pre-IHC pituitary tumor experience, the majority of pituitary tumors seem to be chromophobes. Therefore, the advantages of IHC for pituitary are: 1. Can demonstrate the low levels of granules and hormones in chromophobes. 2. Can further separate pituitary acidophils and basophils into exactly what hormone they are producing. Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Geoff McAuliffe" Cc: Sent: Tuesday, February 17, 2004 8:09 PM Subject: [Histonet] staining of anterior pituitary? > Deal Histonetters: > > I need the experience and opinions of those of you who do clinical > work. What sort of staining is used for anterior pituitary glands these > days? Immuno looking for specific cell types or is there still some > PAS+Orange G, Azan, Herlant's, etc. staining going on? > Thanks! > > Geoff > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histolog <@t> fcv.unl.edu.ar Sat Feb 28 09:04:32 2004 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Advice.....secondary-biotinylated??? Message-ID: <002601c3fe0c$2cdf4c00$5a0cd2aa@unl.edu.ar> And the concentrated secondary-biotinylated antibodies?? -----Mensaje original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] En nombre de Houston, Ronnie Enviado el: Viernes, 27 de Febrero de 2004 12:12 p.m. Para: 'Jackie.O'Connor@abbott.com'; Ian Montgomery CC: histonet@lists.utsouthwestern.edu Asunto: RE: [Histonet] Advice. I think the important factor though,is that antibodies are aliquoted into useable quantities to negate the freeze- thaw process affecting the antibody's stability. Never had any problems with any antibodies that have been frozen, including some >10 years old (before I get shot down, these antibodies were for research purposes!) Ronnie Houston Director of Anatomic Pathology Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 (804) 287 7906 - fax ronnie_houston@bshsi.com -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Friday, February 27, 2004 9:37 AM To: Ian Montgomery Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Advice. This would be a good time for the manufacturers to chime in. When I worked where we manufactured antibodies (not all for IHC) we followed strict freeze/thaw guidelines for antibodies. The maximum number of freeze thaw cycles for bulk antibodies was 3. After that, the potency of the antibodies deteriorated (per stability testing results). The potency of frozen as well as refrigerated antibodies was carefully monitored by pre-determined testing points to ensure reproducible testing results by customers. The expiration date of refrigerated antibodies and other reagents was determined when the potency started to drop off. The potency of antibodies was tested up to a month after the determined expiration date, so we could ensure customers would continue to get satisfactory results right up to the expiration date. Jackie O' Ian Montgomery Sent by: histonet-bounces@lists.utsouthwestern.edu 02/27/2004 07:32 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Advice. Antibody, store at 4C, do not freeze, stable for 1 year. What's the current thinking. Are these antibodies affected by freezing? Or is it just the company covering themselves against users thaw/freezing. In the past I've just aliquoted and frozen anyway. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ ________________________________________________________ ________________________________________________________________________ ________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KAELPERS <@t> aol.com Sat Feb 28 10:41:14 2004 From: KAELPERS <@t> aol.com (KAELPERS@aol.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] working standards for techs in industry Message-ID: <140.23384331.2d721eaa@aol.com> I work in research and this is something that takes time and study...but can be a great evaluation tool. First start with having your technicians document their daily activities. This gives you an idea of productivity of each of your technicians. Then you can talk with them as a group and individually about what is expected. Trimming - what species or specimen, how many, time spent on each. Most technicians of the same experience level should be within 1 (work order) of each other. Also use this a guide to see if that one person whom is excelling (or speedy) is consistent and providing quality work. If the work of this one individual is good have them explain to the other individuals what makes them so productive - a good trainer for the lab in fact...and less time for individuals who may be struggling for technique reasons.....the thing to enforce is that good things can come from this. Embedding - set standards for unloading, cleaning the processor, reloading, embedding, scrapping, sorting and accessioning blocks, pulling or preparing slides, even retrims. Give them a time frame in which all of this should be complete. Say the standard is 2.0 - 2.5 hours for 2 technicians embedding 300 blocks with the above tasks completed. Then they take their 1st break (start time 7:30, 10 minute break at 9:30-10:00, sounds reasonable to me, then they are refreshed and ready to begin the next task). Microtoming - Again have them document time shaving and number of blocks microtomed and on slides. Try to set a standard.....expect that someone whom is on the microtome (proficient) all day should get between 120-180 blocks shaved and on slides (with the exception of bone and eyes which should be shaved much slower for quality purposes). Shave down next series of blocks while the waterbath is warming and blocks are chilling. As one set of blocks are cut add the next shaved set so that their is a flow to what is occurring. Staining/coverslipping - Number of slides in a period of time...ex. 300 slides in 2 hours. Use QC of slides as a good evaluation tool as this should be done prior to submission to the pathologist anyway. Log slides nonacceptable and document the recut and artifact...great evaluation tool for correcting errors. Technicians initially may not like it but if they see that it is a quality issue they would rather it be the manager than the pathologist you can bet the bank on that. I think the key to success (throughout the evaluation period) is not having your technician trying to do too many different task throughout the day. This is when you start to see unproductive times or confusion about what is actually occurring in the laboratory. This can or will change with proficient and self conscious staff over time. Another important thing is to have all things pertinent to the study done prior to the start. I call this study preparation - cassettes, slides and all paperwork prepared before the start of the study so that people are not waiting to use the printers at the same time to print their cassettes or slides prior to working on their specimens - also avoids any transcription errors with people constantly changing the printers. Another thing to consider is having staff validate or do verification first thing in the morning, change out decal or solutions...those type things that are quick and over....suggest that the person whom may do this put their blocks to be cut on ice and set up their waterbath first so that those things that require time are occurring while they are concentrating on getting other things done in the lab - not change out decal, then set up waterbaths and go sit at your desk or 15-20 minutes. It can be very difficult to teach someone how to use their time effectively in the laboratory without just getting them accustomed to a standard or set expectations - as sometimes self motivation is the issue. Hope this helps, and I would gladly take suggestions as anything is helpful for productivity and evaluation! lge From jnocito <@t> satx.rr.com Sat Feb 28 14:37:03 2004 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies References: Message-ID: <00a701c3fe3a$9fe8d3a0$70494542@satx.rr.com> Cindy, we make one cassette for each container. Sometimes there are 3 biopsies in one container. Most of the times the urologists that send us cases submit 12 containers per case. We now have a client that requested 16-vial biopsy kits (yea), but we haven't received any from him yet (thank God). Each container is labeled to a different area, so identification is not a problem with us. Just the gazillion number of slides per case. Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas ----- Original Message ----- From: "Cindy DuBois" To: "Histonet" Sent: Thursday, February 26, 2004 11:33 AM Subject: [Histonet] Prostate Needle Biopsies > I just love this list. It has helped me so much to do the best job I can. > Now to my question: > How do other labs prepare their prostate biopsies? I am talking about > do you separate each biopsy into its' own cassette (whether they are > received this way or not)? Or do you put everything that is received in one > specimen bottle into one cassette (inking them)? > > We have a debate going on at our lab right now as to which way is best. > > I like to know when I am embedding that there will only be one biopsy in > each cassette rather than having to look up the case to find out how many > there are suppose to be and making sure I get them all in the mold and on > the same plane. I have one pathologist who agrees with me. > > My co-workers and the PA want them one block per each container so there are > less blocks to process and cut. > > Our other two pathologists say they don't have a preference. > > I would like to know what the consensus is on these two methods, or does > anyone else have a better way of processing these things. > > Thank you in advance for your help in this matter, > > Cindy DuBois, HT ASCP > DELTA PATHOLOGY ASSOC. > STOCKTON, CA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> earthlink.net Sat Feb 28 16:46:17 2004 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Re: Histonet Digest, Vol 3, Issue 36 In-Reply-To: References: Message-ID: <6.0.0.22.1.20040228164052.01aebc90@pop.earthlink.net> Alan, I've never really had a problem with that. I've seen problems similar to those described, however they were always caused by someone doing something like not using a tamper while embedding the tissue or if a sponge was used instead of a tissue or tea bag for processing. If the tissue is properly treated there will be no problem. I've seen 5-7 pieces in a block with no tissue loss even on recuts. The trick is to be REALLY careful. Don't believe everythnig you read. Amos Brooks At 12:00 PM 2/28/2004, you wrote: >Message: 3 >Date: Fri, 27 Feb 2004 14:04:50 -0500 >From: ALAN MEEKER >Subject: [Histonet] Prostate Needle Biopsies >To: histonet@lists.utsouthwestern.edu >Message-ID: <3d67ee3db686.3db6863d67ee@jhmimail.jhmi.edu> >Content-Type: text/plain; charset=us-ascii > >Regarding the treatment of prostate needle biopsies, a paper recently >appeared in the journal UROLOGY addressing this question. Here is the >reference: > >Individual submission and embedding of prostate biopsies decreases rates >of equivocal pathology reports. Chakshu Gupta, Jian Z. Rena and Kirk J. Wojnoa > >Urology Vol. 63, No.1, January 2004, pp. 83-86 > > >This is the Conclusion from the abstract: > >"Multiple needle biopsies submitted in 1 to 2 containers tend to entangle >and fragment and are difficult to embed in a single plane during >processing. The resulting loss of tissue surface area makes a definitive >diagnosis difficult on small foci of atypical glands, resulting in >equivocal pathology reports. The results of our study indicate that >individual submission and processing of prostate biopsies in 6 to 12 >container kits reduces the monthly rates of equivocal diagnoses." > >-Alan Meeker From RSRICHMOND <@t> aol.com Sat Feb 28 23:59:54 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: <166.2c36c651.2d72d9da@aol.com> Cindy DuBois, HT ASCP at Delta Pathology Associates in Stockton, California, asks about embedding of prostate needle biopsy specimens. A few words from the pathologist who has to read them: I want to see the entire length of every specimen submitted, since prostate cancer is often focal and present in very small quantities. When you gross the specimen, you need to record the length of each specimen (the big prostate mills are very careful about this). I'll be measuring the length of specimens on the slide, and the length of the tumor present within them. Tamping the individual specimens flat in the embedding mold is essential. I'd prefer you use as few cassettes as possible, but it's more important that I see the entire length of each specimen than that you conserve cassettes and slides. Some labs specify no more than two cores per block. I'm going to want a high molecular weight cytokeratin (34BE12) immunostain on about one case in ten. I need to see the section that's right next to the H & E section I'm looking at, so you need to cut extra unstained slides on every case. All prostate biopsy specimens look alike in the gross, and you need to take extraordinary care not to mix up patients, particularly if you're accessioning nothing else but prostate biopsy specimens. Cassettes of different colors, indexed to slides of the same colors, cost nothing extra and help prevent mixups. So does the use of colored marking inks. Bob Richmond Samurai Pathologist Gastonia NC From becky.garrison <@t> jax.ufl.edu Sun Feb 29 11:49:35 2004 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: <38F928258973D711977200D0B7DB9D74018DE427@mail.umc.ufl.edu> Just this week, our Urology Center, in reviewing its QA measures, wanted to know if its Ok for them to ink the specimen by color to reduce specimen ID mix ups. My first reaction was no because of concerns that unknown inks might interfere with fixation and ultimately IHC and the possibility of crush atefact introduced by "non-pathology" hands. In reading messages to this list, apparently there are additional measures taken to prevent specimen ID mix ups. Any other Pathology labs receiving prostate biopsies already inked (or perhaps the formalin tinted) by the Urologist?? Any other measures taken (in addition to the standard precautions of inspecting each container for correct patient/specimen ID before submission to Pathology? All input is appreciated. Becky Garrison Pathology Supervisor Jacksonville, FL -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: Sunday, February 29, 2004 1:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Needle Biopsies Cindy DuBois, HT ASCP at Delta Pathology Associates in Stockton, California, asks about embedding of prostate needle biopsy specimens. A few words from the pathologist who has to read them: I want to see the entire length of every specimen submitted, since prostate cancer is often focal and present in very small quantities. When you gross the specimen, you need to record the length of each specimen (the big prostate mills are very careful about this). I'll be measuring the length of specimens on the slide, and the length of the tumor present within them. Tamping the individual specimens flat in the embedding mold is essential. I'd prefer you use as few cassettes as possible, but it's more important that I see the entire length of each specimen than that you conserve cassettes and slides. Some labs specify no more than two cores per block. I'm going to want a high molecular weight cytokeratin (34BE12) immunostain on about one case in ten. I need to see the section that's right next to the H & E section I'm looking at, so you need to cut extra unstained slides on every case. All prostate biopsy specimens look alike in the gross, and you need to take extraordinary care not to mix up patients, particularly if you're accessioning nothing else but prostate biopsy specimens. Cassettes of different colors, indexed to slides of the same colors, cost nothing extra and help prevent mixups. So does the use of colored marking inks. Bob Richmond Samurai Pathologist Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo20 <@t> hotmail.com Sun Feb 29 12:21:12 2004 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] (no subject) Message-ID: Hi Cindy! We also receive quite a few prostate biopsies A-M, but mostly A-F. Anyway, the policy at our institution is no more than 3 biopsy pieces per cassette, so if an A prostate biopsy contained 7 cores, then the PAs would make two cassettes, one with 3 and other with 4 pieces. As a tech, I find this a little difficult, since both A blocks would have to be embedded together to ensure that 7 pieces were indeed found. The PAs also "ink" the tips of each biopsy with a color dye. We use yellow, black, blue and green. We found that the red ink color bled out to other biopsies. We do this because we sometimes accession several prostate cases back to back, and although they are grossed at different work stations, this is another way to ensure that the accessioning is correct and that patients did not inadvertently get reversed. Paula Wilder St. Joseph Medical Center Towson, MD. 21204 _________________________________________________________________ Find and compare great deals on Broadband access at the MSN High-Speed Marketplace. http://click.atdmt.com/AVE/go/onm00200360ave/direct/01/ From gudrun.lang <@t> aon.at Sun Feb 29 13:16:55 2004 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Acetylcholinesterase -Kit? Message-ID: <001601c3fef8$98178a90$eeeea8c0@SERVER> Can anyone recommend a Kit to perform Acetylcholinesterase on rectum biopsies? Can anyone tell me a substitute for iso-OMPA with this reaction? thanks in advance Gudrun Lang, Austria From mariatere <@t> infovia.com.ar Sun Feb 29 20:24:13 2004 From: mariatere <@t> infovia.com.ar (Teresa Dominguez) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Hi everyone! Message-ID: <000a01c3ff34$4bafcaa0$277f46c8@FAMILIA> Hi All, I am a new suscriber in this list, I work in a Pathology Lab at the Hospital of the city where I live. I am glade to be here, and I would like to share with all of you my experiences, and ask to you whatever I need as an advice. See you in the list messages, bye for now, Maria P.S: sorry if I write something wrong, I am still studying English... H.T Maria T Dominguez Anatomy Pathology Service Hospital Regional R?o Grande, R?o Grande, Tierra del Fuego, Argentina 54- 02964-422086/88 Ext. 143 From RSRICHMOND <@t> aol.com Sun Feb 29 21:23:47 2004 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Prostate Needle Biopsies Message-ID: <1db.1b50c90c.2d7406c3@aol.com> Becky Garrison in Jacksonville FL observes: >>Just this week, our Urology Center, in reviewing its QA measures, wanted to know if it's OK for them to ink the specimen by color to reduce specimen identification mixups. My first reaction was no because of concerns that unknown inks might interfere with fixation and ultimately IHC and the possibility of crush artefact introduced by "non-pathology" hands.<< I agree - no. I want them to handle the specimen as little as possible - the ink needs to be added after fixation. They could use colored pens to mark the outsides of the containers with a set of laboratory-prescribed colors that the lab would use to select cassette and slide frosting colors. I know that there are services that pre-ink the specimens, but I've never actually seen it done. Bob Richmond Samurai Pathologist Gastonia NC From JWEEMS <@t> sjha.org Sun Feb 29 21:39:37 2004 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Hi everyone! Message-ID: <8C54DF3F71F6E34A80B1DE628C7EF4DD01DD63E1@exch2.sjha.org> Welcome, Maria! Don't worry about your English. We're still studying too! And we still get it wrong. Just hop in when you have a question. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 2/29/2004 9:24 PM Subject: [Histonet] Hi everyone! Hi All, I am a new suscriber in this list, I work in a Pathology Lab at the Hospital of the city where I live. I am glade to be here, and I would like to share with all of you my experiences, and ask to you whatever I need as an advice. See you in the list messages, bye for now, Maria P.S: sorry if I write something wrong, I am still studying English... H.T Maria T Dominguez Anatomy Pathology Service Hospital Regional R?o Grande, R?o Grande, Tierra del Fuego, Argentina 54- 02964-422086/88 Ext. 143_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histon Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From scoop <@t> mail.nih.gov Sun Feb 29 21:41:34 2004 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:37 2005 Subject: [Histonet] Inga Hansson and ED1 antibody Message-ID: Hi. I'm writing to say thank you to Inga Hansson who told me about using the ED1 antibody from Serotec for microglia on FFPE tissue with citrate antigen retrieval. It also works great on bone marrow macrophages and knowing about it saved me a huge amount of time. I can't find Inga's email address to email her directly, so if you're out there Inga, thanks. -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892