[Histonet] Trypan Blue Removal For Protein Analysis

[Timothy Lewis Liparulo]

I am currently using Trypan Blue as a measure for injury in rat 
skeletal muscle cells.  The animals were preinjected with the dye 24 
hrs prior to sacrifice. I would like to perform a protein analysis on 
the tissue but am afraid that the dye in the tissue will be problematic 
for western blot analysis.  Does anyone know of a way to remove the dye 
pigment without significantly affecting the integrity of the muscle 
protein?