[BULK] - [Histonet] Coverslipping tape

Fred Underwood funderwood <@t> mcohio.org
Fri Aug 27 14:35:38 CDT 2004


Hi Pam,

Immerse the slide in acetone for about 5 minutes.  The film will swell
up and come right off the slide.  Swish the slide in alcohol then back
into xylene(a couple of changes) and coverslip.

Fred

>>> pam plumlee <paw555 <@t> yahoo.com> 08/27/04 12:04PM >>>
Hi all:  I know this has been asked before, is there a
quick fix for removing tissue from coverslipping tape,
reattaching to the slide, covering with a new glass
coverslip so the tissue can be photographed?  Also,
does anyone know a good alertnate fixative for osmium
tetroxide?  We are trying to keep it out of the lab. 
We have been asked to use it for a post-fix for fatty
tissue and on an EM processor.  Thanks, Pam


		
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