[Histonet] alcian blue pH

[Tom Schaer]

Greetings List:

I would like to stain some intervertebral disc x-sections (rat, decalcified) with alcian blue.  Interest in cellular morphology of nucleus pulposus, transition and annulus cells.

What pH of the stain should I use.

Many thanks, 

tom
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Thomas P. Schär, VMD
Asst  Prof  School of Biomedical Engineering, Drexel University
& Comparative Orthopaedic Research & Tissue Engineering
Department of Clinical Studies
University of Pennsylvania New Bolton Center
382 West Street Road
Kennett Square, PA 19348
tel. 610-444-5800 (x6261 office)
tel. 610-444-5800 (x6131 lab)
fax. 610-925-8100
tpschaer <@t> mail.vet.upenn.edu
http://www2.vet.upenn.edu/labs/corl/drtps.html