[Histonet] Mallory's PTAH

[UniPath IHC]

I actually already tried to use formalin-fixed muscle.  You're exactly
right, it sectioned great, but I could never get any decent staining.  I
tried post-fixing for 24 hrs. in Zenker's vs. post-fixing for 3 Hrs in
Zenker's at 56 degrees.  I tried all three protocols in Sheehan and the
protocol in Carson.  I'm at a loss for what to do next if I can't make the
Zenker fixed muscle work.  At least I've learned a lot about the stain.  :-)

 

Brianna Jackson, BS, QIHC

UniPath, LLC

Denver, CO