[Histonet] Problems with Fluorojade staining

[Barlow, Gillian]

Hi there

Does anyone out there have experience with Fluorojade staining of
paraformaldehyde-fixed, paraffin-embedded mouse brains?  We are getting
quite high background following the published protocol, can anyone recommend
changes/modifications?  Sections are only 4 microns.

Many thanks!
Gillian

Gillian M. Barlow, PhD
Postdoctoral Fellow
Laboratory of Julie Korenberg, PhD, MD
Cedars-Sinai Medical Center
Davis Bldg, Lab 2007
110 George Burns Rd
Los Angeles, CA 90048

Phone: (310) 423 7650
Fax: (310) 423 0302