[Histonet] Buffer vs Water

[Amos Brooks]

Hi,
         You would be better off using buffer (PBS or TBS). The reason as 
it was described to me was that the antibodies are more likely to locate 
their binding sites if they don't have a lot of tonicity and VanDerwalls 
force induced agitation of the solutions. The pH of the washes should be as 
close to the pH of the antibody solution. If the antibody has a strong 
affinity to the antigen then you may not notice any difference. It becomes 
important when there isn't a strong attraction between the two.
Hope this helps,
Amos Brooks

At 10:14 AM 4/28/2004, you wrote:
>Message: 1
>Date: Tue, 27 Apr 2004 13:07:09 -0400
>From: Kristopher.Kalleberg <@t> unilever.com
>Subject: [Histonet] (no subject)
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <iss.ca.408e933d.280.bb <@t> edg-001.ed.u4267.unilever.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>When performing IHC, and the stain is combined with water, are better results
>achieved when rinsed with water or should PBS be used