[Histonet] staining frozen sections

[Michael Hetzer]

Lori,

Staining frozen sections is a lot like staining paraffin sections
except that there is no need to dewax. (OCT will rinse off during the
staining process.) Dehyrdration is the critical factor with fresh frozen
tissue. For fatty sections, be sure that the slides are dry before
fixing them in 95% ETOH for  three minutes. (You can fix the slides in
10% NBF but then you must really be sure to thoroughly rinse the slides
before proceeding with the staining process.) We use Harris Hematoxylin
and alcoholic Eosin and stain regressively but you can use your own H&E
protocol. 

Mike Hetzer
Mohs Surgery
Madison, WI