[Histonet] RE: Histonet Digest, Vol 5, Issue 40
Robert Fauck
Robert.Fauck <@t> ccdhb.org.nz
Mon Apr 26 18:17:51 CDT 2004
Hi Pam,
Do you know by chance also some job vacancies in the Middle East? Or a
good website for searching for jobs in the Middle East?
Thanking you in advance for some info.
Cheers,
Robert Fauck
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, 27 April 2004 5:03 a.m.
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 5, Issue 40
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Today's Topics:
1. Job Opportunities in Histology Latest Update 04/25/04
(Pam Barker (extension 234))
2. TISSUE SPECIMENS WANTED (Banjo Adesuyi)
3. Re: Biopsy processing schedule (jhnspam <@t> aol.com)
4. detect primordial germ cells in PFA-fixed marsupial
embryos...? (Bruce Abaloz)
5. Basic Info (Asarpota, Hitesh (MED, Intern))
6. RE: beta galactosidase nuclear counterstain (C.M. van der Loos)
7. No Posting Ability (Dawson, Glen)
8. OCT definition (Phil Bergin)
9. RE: OCT definition
(Marshall Terry Dr, Consultant Histopathologist)
10. Re: OCT definition (Petia P Stefanova)
11. Re: OCT definition (Bryan Hewlett)
12. Re: OCT definition (Kathleen Spencer)
13. Re: Awards / Scholarships (SUSANLMCCOY <@t> aol.com)
14. Re: beta galactosidase nuclear counterstain (Gayle Callis)
15. OCT definition (Gayle Callis)
16. Re: OCT definition (Don.Birgerson <@t> leica-microsystems.com)
----------------------------------------------------------------------
Message: 1
Date: Sun, 25 Apr 2004 13:16:31 -0400
From: Pam Barker (extension 234) <pam <@t> ategra.com>
Subject: [Histonet] Job Opportunities in Histology Latest Update
04/25/04
To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1BHnB3-00056T-01 <@t> harrier.mail.pas.earthlink.net>
Content-Type: Text/Plain
Hi Histonet, Here is the latest update on opportunities with some of my
best clients throughout the US who are seeking Histology Supervisors,
Histo Technologists and Histo Technicians. These are positions as
direct employees of our client. There are fulltime 40 hour per week
positions.. As a direct employee of one of our clients you will be
provided with full benefits including Health Insurance, Vacation, Sick
Pay, Relocation money and a lucrative sign-on bonus. I have part time
and temporary positions as well.
I have supervisory, team lead and bench positions. These positions
require HTL or HT certification or registry eligibility.
Here are some of my HOTTEST Histology Supevisory positions:
1. Colorado - Histology Lab Manager
2. Connecticut - Histology Manager
3. Connecticut - Histology Supervisor
4. Maryland - AP Supervisor (Histology/Cytology)
5. California - Histology Technical Coordinator
Here are some of my HOTTEST Histo Tech bench positions:
1. Florida - Histo Tech (full time and part time positions)
2. Maine - Histo Tech
3. Georgia - Histo Tech
4. Pennsylvania - Histo Tech
5. Oregon - Histo Tech
6. Rhode Island - MOHS Tech
7. Wisconsin - MOHS Tech
8. California - Histology Trainer
9. California - Histo Tech
10. California - Lead Histo Tech
11. Nevada - Histo Tech
12. Maryland - Histo Tech
13. Massachusetts - Histo Tech (part time)
14. Colorado - Histo Tech
15. Massachusetts - MOHS Tech
If you are interested in these jobs, please CALL ME ASAP at 800 466 9919
x234. To speed things up, please also send me a copy of your resume, (if
you haven't already done so). If you are interested in jobs outside the
above-mentioned areas, please send me your resume as well. I have
clients throughout the US. I will keep your resume confidential and will
not release it to anyone without your permission (This is Ategra policy
as well as my own). My services are at no charge to you.
Of course, you may be happy in your present job, but it never hurts to
to keep an eye open. Also, if you have friends/peers who might be
interested as well, if you could pass my query & name on to them I'd be
very grateful.
However, if you are interested in any of the jobs above, please call me.
Thank You !!
Pam - 800 466 9919 ext 234
---------------------------------------------------------
Ategra Systems Inc
Specialists in Permanent & Contract Staffing
Learn More About Ategra:
<http://www.ategra.com/service/service.html>
Pam Barker
Senior Lab Recruiter
Ategra Systems Inc
Specialists in Permanent & Contract Staffing
7085 University Blvd.
Winter Park, FL 32792
VOICE: 407-671-5800 ext 234
TOLLFREE: 800-466-9919 ext 234
EMAIL: pam <@t> ategra.com <mailto:pam <@t> ategra.com>
To Learn More About Ategra:
http://www.ategra.com
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Message: 2
Date: Mon, 26 Apr 2004 01:03:47 +0300
From: "Banjo Adesuyi" <badesuyi <@t> hotmail.com>
Subject: [Histonet] TISSUE SPECIMENS WANTED
To: histonet <@t> lists.utsouthwestern.edu
Cc: badesuyi <@t> hotmail.com
Message-ID: <BAY18-F93x6zyCqvDrz0000fb8e <@t> hotmail.com>
Content-Type: text/plain; format=flowed
Hi,
Please I will appreciate it, If you can assist me in getting the
two
tissue specimens listed below for my practical test.
The tissue specimens are;
1) ARTERY - complete cross section.
2) OVARY - 1.0 x 1.0cm, to include cortical
surface along one entire edge.
Wishing you all God's guideance and protection, amen.
BANJO ADESUYI, B.Sc, HT(ASCP)
PATHOLOGY DEPARTMENT,
VAL VERDE REGIONAL MEDICAL CENTER,
801 BEDELL AVENUE, DEL RIO, TEXAS 78840.
_________________________________________________________________
Tired of spam? Get advanced junk mail protection with MSN 8.
http://join.msn.com/?page=features/junkmail
------------------------------
Message: 3
Date: Sun, 25 Apr 2004 21:10:26 EDT
From: jhnspam <@t> aol.com
Subject: Re: [Histonet] Biopsy processing schedule
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1dc.1fde35e6.2dbdbb82 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
What kind of tissue processor are you using?
Pam Johnson
------------------------------
Message: 4
Date: Mon, 26 Apr 2004 12:09:51 +1000
From: Bruce Abaloz <brucea <@t> unimelb.edu.au>
Subject: [Histonet] detect primordial germ cells in PFA-fixed
marsupial embryos...?
To: histonet-bounces <@t> lists.utsouthwestern.edu,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <p06002004bcb21f79f965@[128.250.104.180]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Hi
I have been using alkaline phosphatase histochemistry to detect
primordial germ cells in PFA-fixed marsupial embryos. I use 1mg/ml
Fast Blue salt and 1mg/ml Naphthol AS phosphate sodium salt in 0.2M
Tris buffer, pH 9.4. The staining is good but I have been told that
it is not permanent. Ideally, I would like to dehydrate these stained
specimens, embed them in paraffin and then section and stain them
immunohistochemically for other germ cell markers and several growth
factors. Does anyone have a protocol utilising alkaline phosphatase
histochemistry that would allow me to do this? Thanks for any advice
in advance,
Dr Danielle Hickford
Research Fellow
Department of Zoology, University of Melbourne,
Australia 3010.
hickford <@t> unimelb.edu.au
--
BRUCE ABALOZ PH:61383446282
HISTOLOGIST FAX:61383447909
DEPT.of ZOOLOGY EMAIL: brucea <@t> unimelb.edu.au
THE UNIVERSITY Of MELBOURNE.
VICTORIA.AUSTRALIA 3010
Nobody can make you feel inferior without your
permission. - Eleanor Roosevelt
DANCE LIKE NO-ONE'S WATCHING
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Message: 5
Date: Mon, 26 Apr 2004 11:32:47 +0200
From: "Asarpota, Hitesh (MED, Intern)" <Hitesh.Asarpota <@t> med.ge.com>
Subject: [Histonet] Basic Info
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6EDD9DC298BF9C48AC3EF66EA92596BC11819C2D <@t> frbucmsx03medge>
Content-Type: text/plain; charset="iso-8859-1"
Hi..
I am a management student currently on an internship at GE Healthcare.
Part
of my work is researching on anti-bodies and reagents. Could someone
please
guide me to an appropriate URL wherein I could look for detailed
(although
comprehensible to a layman!) info on the same?
Thank you!
Regards,
Hitesh Asarpota
----------------------------------------------------------
GE HealthCare
Business Development
Tel: +33 01 30 70 9224
Cell: +33 06 1171 4370
----------------------------------------------------------
------------------------------
Message: 6
Date: Mon, 26 Apr 2004 11:37:33 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: beta galactosidase nuclear counterstain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2d95662d5286.2d52862d9566 <@t> amc.uva.nl>
Content-Type: text/plain; charset=us-ascii
Hi,
Using X-gal as substrate and ferri/ferro-cyanide as chromogen for
b-galactosidase activity you obtain a turquoise/blue reaction product
that survives everything: alcohol, xylene, cooking in citrate, in situ
procedure.....
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands
---- Original Message -----
>From Shaumik Adhya <shaumik.adhya <@t> ucl.ac.uk>
Date Fri, 23 Apr 2004 10:46:59 +0100
To histonet <@t> lists.utsouthwestern.edu
Subject [Histonet] beta galactosidase nuclear counterstain
Hi Histonetters,
I'm trying to stain myocardium for beta galactosidase (resulting in a
blue
precipitate), and have been trying to use Nuclear Fast Red as a nuclear
counterstain - whilst this gives me reasonable nuclear staining, the
process
of dehydrating and mounting makes me lose all the beta gal. If I use an
aqueous mount such as gycergel, the Nuclear Fast Red dissolves out over
the
course of a week, which is less than ideal.
My protocol for dehydrating and mounting is:
10 quick dips in 70% ethanol
2 min in 100% ethanol
5 min in 100% ethanol
5 min in Histoclear
2 min in Histoclear
DPX to coverslip with.
I'm wondering what people use as a nuclear counterstain for beta-gal,
and if
you had any tips or advice. Thanks
------------------------------
Message: 7
Date: Mon, 26 Apr 2004 07:28:34 -0500
From: "Dawson, Glen" <GDawson <@t> Milw.Dynacare.com>
Subject: [Histonet] No Posting Ability
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B915727D2 <@t> MILW_MAIL1>
Content-Type: text/plain; charset="iso-8859-1"
Sorry,
Just testing to see if anything I post makes it to the hist-net.
G. Dawson
------------------------------
Message: 8
Date: Mon, 26 Apr 2004 15:28:13 +0200
From: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>
Subject: [Histonet] OCT definition
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1082998801.26092.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi everyone,
Does anyone know what OCT stands for, and do people ever define it in
papers
or just call it OCT compound? Seems a silly question- but what do
people
normally do? We normally just call it OCT compound, but I have been
asked
to define it, by the copy editor for a paper.
Thanks
Phil
------------------------------
Message: 9
Date: Mon, 26 Apr 2004 14:40:08 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] OCT definition
To: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F02DC9DED <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
Optimum cutting temperature
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Phil Bergin [mailto:philip.bergin <@t> microbio.gu.se]
Sent: 26 April 2004 14:28
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] OCT definition
Hi everyone,
Does anyone know what OCT stands for, and do people ever define it in
papers
or just call it OCT compound? Seems a silly question- but what do
people
normally do? We normally just call it OCT compound, but I have been
asked
to define it, by the copy editor for a paper.
Thanks
Phil
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mon, 26 Apr 2004 09:41:45 -0400
From: "Petia P Stefanova" <pstefanova <@t> sten.sunnybrook.utoronto.ca>
Subject: Re: [Histonet] OCT definition
To: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005d01c42b94$3aad5a10$9d194c8e <@t> WS21203>
Content-Type: text/plain; charset="iso-8859-1"
HI Phil,
O.C.T. means optimal cutting temperature.
Good luck!
Petia
----- Original Message -----
From: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, April 26, 2004 9:28 AM
Subject: [Histonet] OCT definition
> Hi everyone,
>
>
>
> Does anyone know what OCT stands for, and do people ever define it in
papers
> or just call it OCT compound? Seems a silly question- but what do
people
> normally do? We normally just call it OCT compound, but I have been
asked
> to define it, by the copy editor for a paper.
>
>
>
> Thanks
>
>
>
> Phil
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 11
Date: Mon, 26 Apr 2004 09:48:09 -0400
From: "Bryan Hewlett" <bhewlett <@t> cogeco.ca>
Subject: Re: [Histonet] OCT definition
To: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701c42b95$30865d10$6500a8c0 <@t> mainbox>
Content-Type: text/plain; charset="iso-8859-1"
Hi Phil,
OCT stands for Optimal Cuttting Temperature compound.
Check out the following;
http://www.tedpella.com/msds_html/27050msd.htm
Regards,
Bryan
----- Original Message -----
From: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, April 26, 2004 9:28 AM
Subject: [Histonet] OCT definition
> Hi everyone,
>
>
>
> Does anyone know what OCT stands for, and do people ever define it in
papers
> or just call it OCT compound? Seems a silly question- but what do
people
> normally do? We normally just call it OCT compound, but I have been
asked
> to define it, by the copy editor for a paper.
>
>
>
> Thanks
>
>
>
> Phil
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 12
Date: Mon, 26 Apr 2004 09:38:17 -0500
From: Kathleen Spencer <kspencer <@t> utmem.edu>
Subject: Re: [Histonet] OCT definition
To: Phil Bergin <philip.bergin <@t> microbio.gu.se>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5B7FAC88-978F-11D8-A6E7-000393967904 <@t> utmem.edu>
Content-Type: text/plain; format=flowed; charset=US-ASCII
Optimal Cutting Temperature. Never did make sense to me.
-Kathleen
On Monday, April 26, 2004, at 08:28 AM, Phil Bergin wrote:
> Hi everyone,
>
>
>
> Does anyone know what OCT stands for, and do people ever define it in
> papers
> or just call it OCT compound? Seems a silly question- but what do
> people
> normally do? We normally just call it OCT compound, but I have been
> asked
> to define it, by the copy editor for a paper.
>
>
>
> Thanks
>
>
>
> Phil
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Mon, 26 Apr 2004 11:25:07 -0400
From: SUSANLMCCOY <@t> aol.com
Subject: Re: [Histonet] Awards / Scholarships
To: lpwenk <@t> covad.net, histonet <@t> lists.utsouthwestern.edu
Message-ID: <01F6E1D9.6450FC86.3C887B41 <@t> aol.com>
Content-Type: text/plain; charset=iso-8859-1
HI PEGGY,
I AGREE, APPLY! APPLY! APPLY!
THANK YOU FOR YOUR MESSAHE TO THE MEMBERS.
SUSAN
------------------------------
Message: 14
Date: Mon, 26 Apr 2004 09:37:44 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] beta galactosidase nuclear counterstain
To: Shaumik Adhya <shaumik.adhya <@t> ucl.ac.uk>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040426093744.00be7e08 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Just air dry sections after final water rinse (after nuclear fast red
counterstain). You can do this in front of a fan and up to 2 hours.
Mount
a permanent coverslip and avoid dehydration through alcohols, etc.
At 10:46 AM 4/23/2004 +0100, you wrote:
>
>
>Hi Histonetters,
>
>I'm trying to stain myocardium for beta galactosidase (resulting in a
blue
>precipitate), and have been trying to use Nuclear Fast Red as a nuclear
>counterstain - whilst this gives me reasonable nuclear staining, the
process
>of dehydrating and mounting makes me lose all the beta gal. If I use
an
>aqueous mount such as gycergel, the Nuclear Fast Red dissolves out over
the
>course of a week, which is less than ideal.
>
>My protocol for dehydrating and mounting is:
>
>10 quick dips in 70% ethanol
>2 min in 100% ethanol
>5 min in 100% ethanol
>5 min in Histoclear
>2 min in Histoclear
>DPX to coverslip with.
>
>I'm wondering what people use as a nuclear counterstain for beta-gal,
and if
>you had any tips or advice. Thanks
>
>Shaumik
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 15
Date: Mon, 26 Apr 2004 10:08:58 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] OCT definition
To: "Phil Bergin" <philip.bergin <@t> microbio.gu.se>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040426100858.00be7e08 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Optimal Cutting Temperature, clearly stated on the dispensing bottle.
Dr.
McCormick was in on the development of this cryoembedding media with
Miles
aka Tissue Tek, now Sakura Finetek. The bottle also says it contains
polyvinyl alcohol, polyethylene glycol and inert ingredients. The PVA
and
PEG concentration and molecular weights are proprietary.
Via private discussion with him, he said one could adjust the PVA and/or
PEG to give different cutting qualities at different temperatures and
different hardness to the media. The mixture we buy today is what they
came
up with. It has a good temperature range (we use anywhere from -16C to
-35C). It is designed to surround the tissue for support and not
infiltrate (as one does with paraffin). Some do this, but it must be
pointed out the if the MW of the major ingredients is large,
infiltration
would probably be minimal.
Interesting, I have never been asked to define OCT in great detail for
any
publication! Usually it is stated tissue placed in O.C.T. cryoembedding
media (be sure to put in trademark, Sakura Finetek, Torrance CA) and
snap
frozen at certain temperature. Methinks your editor asketh for too much
when it is not really necessary to explain OCT is such detail.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 16
Date: Mon, 26 Apr 2004 11:16:59 -0500
From: Don.Birgerson <@t> leica-microsystems.com
Subject: Re: [Histonet] OCT definition
To: Kathleen Spencer <kspencer <@t> utmem.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu, Phil Bergin
<philip.bergin <@t> microbio.gu.se>
Message-ID:
<OF2D079602.87B5A865-ON86256E82.0057B612-86256E82.0059592F <@t> e-leica.com>
Content-Type: text/plain; charset=us-ascii
Kathleen,
The original compound came in three temperature ranges. O.C.T. Roman
numeral( l) for 0 to -15 degrees, , O.C.T.( II ) for -15 to -30, and
O.C.T.( III ) for -30 to -60. Today you have only one choice. I think
"O.C.T." Compound was a copyright name at one time and competitors now
refer to "freezing compound" for their products.
Don Birgerson
Leica Microsystems
Technical Assistance Center
Don.Birgerson <@t> Leica-Microsystems.Com
1-800-248-0123 ext 5918
Kathleen Spencer
<kspencer <@t> utmem.edu> To:
Phil Bergin <philip.bergin <@t> microbio.gu.se>
Sent by: cc:
histonet <@t> lists.utsouthwestern.edu
histonet-bounces <@t> lists.utsouth Subject:
Re: [Histonet] OCT definition
western.edu
04/26/2004 09:38 AM
Optimal Cutting Temperature. Never did make sense to me.
-Kathleen
On Monday, April 26, 2004, at 08:28 AM, Phil Bergin wrote:
> Hi everyone,
>
>
>
> Does anyone know what OCT stands for, and do people ever define it in
> papers
> or just call it OCT compound? Seems a silly question- but what do
> people
> normally do? We normally just call it OCT compound, but I have been
> asked
> to define it, by the copy editor for a paper.
>
>
>
> Thanks
>
>
>
> Phil
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 5, Issue 40
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