[Histonet] beta galactosidase nuclear counterstain
Shaumik Adhya
shaumik.adhya <@t> ucl.ac.uk
Fri Apr 23 04:46:59 CDT 2004
Hi Histonetters,
I'm trying to stain myocardium for beta galactosidase (resulting in a blue
precipitate), and have been trying to use Nuclear Fast Red as a nuclear
counterstain - whilst this gives me reasonable nuclear staining, the process
of dehydrating and mounting makes me lose all the beta gal. If I use an
aqueous mount such as gycergel, the Nuclear Fast Red dissolves out over the
course of a week, which is less than ideal.
My protocol for dehydrating and mounting is:
10 quick dips in 70% ethanol
2 min in 100% ethanol
5 min in 100% ethanol
5 min in Histoclear
2 min in Histoclear
DPX to coverslip with.
I'm wondering what people use as a nuclear counterstain for beta-gal, and if
you had any tips or advice. Thanks
Shaumik
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