[Histonet] mounting medium

DiCarlo, Margaret MDiCarlo <@t> KaleidaHealth.Org
Thu Apr 22 07:06:22 CDT 2004


Hello Histonetters

My 5 x 7 inch slides are air dried flat for at least a month before I file
them upright.  The problem I'm having is that once the slides are filed some
of the toluene based mounting medium oozes out.  After I clean the slides
off there is usually a big air bubble where the tissue is and I don't like
to re-coverslip for fear of the tissue falling off since it has happened.  I
spoke to a sales rep and he is sending me different mountants to try ( I
assume all xylene based since I coverslip from xylene) but I was wondering
what everyone else uses to prevent this from happening.   Any suggestions to
eliminate this problem?  

Thanks for your help.
Peggy DiCarlo HT (ASCP)

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