[Histonet] MacNeals Tetrachrome stain solution recipe
Gayle Callis
gcallis <@t> montana.edu
Wed Apr 21 12:24:39 CDT 2004
Dear LaCinda,
Here is the recipe you are looking for. I have stained PMMA embedded bone
sections, thicker/ground with this, but I did use 1% formic acid etching
for 30 seconds in ultrasonicator, a very good water rinse after this, then
dry section and immerse. This stain can also be combined with Toluidine
Blue, Sterchi method for even more brilliant results particularly if you
want to demonstrate ostoid!
MACNEAL'S TETRACHROME
STOCK SOLUTION
0.5g methylene blue
0.8g azure A eosinate
0.1g methylene violet CI# 52041
250 ml methanol
250 glycerin
Stir, leave at 50C for 12 hours, then 37C for 3 days. Filter, store in
dark. It is good for up to years! Older solutions stain very well, if
not better.
WORKING STAINING SOLUTION
5 mls stock stain solution, 95mls distilled water
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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