[Histonet] Mesentery tissue
J. A. Kiernan
jkiernan <@t> uwo.ca
Tue Apr 20 09:51:54 CDT 2004
The way I do mesentery is as follows. The
preparations are very well suited to lab
teaching.
1. Remove a rat's small intestine with the
mesentery attached. Rinse in saline
to get rid of blood etc.
2. Put a loop of gut over one end of a
microscope slide, and pull on the
mesentery so that it's stretched over
the slide.
3. Trim round the edges of the glass
with a sharp scalpel or razor blade,
and scrape off any thick bits, such as
larger blood vessels, from the middle
of the slide.
4. Before the preparation dries out
completely, immerse the slide in
a coagulant fixative (Carnoy's is
good) for a few minutes. Store the
slides in 70% alcohol until you're
ready to stain them.
Many methods work really well on such
preparations. Dilute toluidine blue will
provide spectacular metachromatic staining
of mast cells, for example. If you miss
out Step 4 above, you can expose the
preparation to hot formaldehyde vapour
and see the sympathetic nerve fibres by
fluorescence microscopy (a histochemical
test for noradrenaline). You can also use
staining methods for different types of
connective tissue fibres.
--
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan[AT]uwo.ca
http://publish.uwo.ca/~jkiernan/
http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Donna Kumiski wrote:
>
> I am looking for a protocol to stain and mount mesentery.
> In a past discussion I asked about mounting the tissue but I need
> more details about the staining. I would like to do an H&E on the
> tissue then mount it on slides. These slides will be used in a
> Histology
> course for first year medical students
> Donna
>
> ----------------------------------------------
> Donna Kumiski HT (ASCP)
> Medical College of Georgia
> Dept. of Cellular Biology and Anatomy
> CB 1202
> Augusta, Georgia 30912-2000
> (706) 721-6278
> dkumiski <@t> mail.mcg.edu
> -----------------------------------------------
>
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