[Histonet] IF on frozen sections
GUTIERREZ, JUAN
juan.gutierrez <@t> christushealth.org
Tue Apr 20 07:55:17 CDT 2004
I've always used OCT on my skins with no adverse effect. It's so much easier.
Happy cutting,
Juan
-----Original Message-----
From: Gudrun Lang [mailto:gudrun.lang <@t> aon.at]
Sent: Monday, April 19, 2004 11:34 AM
To: Histonetliste
Subject: [Histonet] IF on frozen sections
A question about immunfluorescence on frozen skin sections:
We mount the skin with buffer on the sample-table to freeze it. After sectioning we let the cuts airdry and do the IF (Immunglobulins).
Is there any reason, why we cannot use OCT for mounting? I think sectioning would be easier.
Perhaps someone can help me?
Lang Gudrun
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