[Histonet] tissue slicer

[Katy Whalley]

Hi,

Thanks to everyone for your advice and suggestions. It seems I might have
to re-think the thickness of the slices required - in retrospect, I was
probably aiming too low anyway. Basically, the reason we want to cut them
quickly is in order to culture the slices later, so I'm not sure a
vibratome or cryostat would be appropriate as I think the tissue would
have to be embedded/ frozen first. Overall the 'egg-slicer' or matrix type
of device seem like they may be the best option, but I may try to make my
own, as described by Geoff, to cut costs a bit.

Katy

 Bad vibes, man.
>
> But, Geoff, your device would work, but as you note, not for less
> than 1000 micron slices, definitely not 30 - 300 micron, and there'd
> be more tissue damage from compression. A vibratome is pretty much
> the only choice, there is no "all slices at once" instrument that
> I've seen.
>
> Phil
>
>>Always wondered what a vibratome was.
>>I've got a wifatome.
>>
>>Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
>>  Consultant Pathologist
>>  Rotherham General Hospital
>>  South Yorkshire
>>  England
>>         terry.marshall <@t> rothgen.nhs.uk
>>
>>-----Original Message-----
>>From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu]
>>Sent: 19 April 2004 19:08
>>To: k.whalley <@t> ich.ucl.ac.uk
>>Cc: histonet <@t> lists.utsouthwestern.edu
>>Subject: Re: [Histonet] tissue slicer
>>
>>
>>Hi Katy:
>>
>>     You could buy a Vibratome, a device with a vibrating blade that will
>>cut fixed or unfixed tissue at a thickness you select. I think there are
>>several models and vendors.
>>     Or, you could make an inexpensive device for little more than pocket
>>change. Buy some high-quality double-edge razor blades and some material
>>to use for spacing the blades. For 1 mm or more use square aluminum rod,
>>for 0.5 mm or less use "shim stock". A well-stocked hardware store or
>>maching shop will have these items. Use "super-glue" to glue up a
>>"blade-spacer-blade-spacer-blade ..." tool with as many blades as your
>>project demands. One 'application' of the tool to the sample will give
>>you uniform and reproducable slices.  Be sure to  cut off or mask the
>>edge of the blade not in use so you won't cut yourself.
>>
>>Geoff
>>
>>Katy Whalley wrote:
>>
>>>Hi,
>>>
>>>We are looking for a device which can be used to cut tissue quickly into
>>>slices of an even thickness. We're not sure yet exactly how thick these
>>>will be but something in the range 30-300 microns is likely. My
>>> supervisor
>>>has in mind something in which several blades are attached to a holder
>>>that keeps them the correct distance apart, so that all the slices are
>>> cut
>>>at once. Has anyone ever used/ seen this kind of thing, or anything else
>>>which would do the job?
>>>
>>>thanks,
>>>Katy, UCL
>
> --
> Philip Oshel
> Supervisor, BBPIC microscopy facility
> Department of Animal Sciences
> University of Wisconsin
> 1675 Observatory Drive
> Madison,  WI  53706 - 1284
> voice: (608) 263-4162
> fax: (608) 262-5157 (dept. fax)
>
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