[Histonet] Microwave Processing

[Willis, Donna]

Terry,
Saline will not fix your tissue.  It will only stabilize it.  If you do not
post fix in formalin after stabilizing in saline then your tissue will be
fixed in the alcohol when processed.  Saline works great to firm up the
tissue to assist in dissection.

Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth, Tx

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Marshall
Terry Dr, Consultant Histopathologist
Sent: Friday, April 16, 2004 9:59 AM
To: kevin williams; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Microwave Processing 


Sorry to but in - but on a related question - does anyone do microwave
fixation in saline as described by for instance the Melbourne group, which
involves bringing up to 65C in saline?
Have tried it this week and the sections are even crummier than usual.
When I was in Tasmania, a local private lab. did it and their sections were
fine.

Dr Terry L (why can't I get a good H&E) Marshall, B.A.(Law),
M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: kevin williams [mailto:akwilliams75 <@t> hotmail.com]
Sent: 16 April 2004 15:33
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Microwave Processing and Derm Shaves



   Is anyone out there doing any processing on derm shaves with microwave
   processing? How is it and how are the results?
   Thanks.

   A. Kevin Williams
   Vermont Dermatopathology.
     _________________________________________________________________

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References

   1. http://g.msn.com/8HMBENUS/2752??PS=
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