[Histonet] cutting frozen rat turbs,, a reply

[Gayle Callis]

I am presuming you mean UNDECALCIFIED rat turbinates?  We do mouse
undecalcified turbinates, but need to sue the tape transfer system,
Cryojane from Instrumedics OR you crush delicate turbinate bone, do not get
a flat section that adheres to slide surface hence falling off.  

It requires a d profile (some like c profile) tungsten carbide knife in
extremely sharp condition in order to cut decently through bone. 

Without these tools, we get terrible sections. 

At 09:09 AM 4/16/2004 -0600, you wrote:
>Has anyone out there cut frozen rat turbinates??  We are having trouble
>keeping them on the slides.  Any pointers?
>
>Thanks,
>Antonia
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)