[Histonet] Best Marker for Proliferation?
Jackie.O'Connor <@t> abbott.com
Jackie.O'Connor <@t> abbott.com
Fri Apr 16 07:13:16 CDT 2004
My vote is with Ki67. I work with human xenografts in mice, and you'll
find in a lit search that Ki67 (MIB1) is the most commonly used marker for
proliferation. Most manufacturers antibodies work well with HIER -
tough to find one that is human specific, tho - which means you get
staining in the stroma, but for the most part it's inconsequential.
Jackie O'Connor
"Isaiah G. Schauer" <is135475 <@t> bcm.tmc.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/15/2004 10:41 PM
To: histonet <@t> lists.utsouthwestern.edu
cc:
Subject: [Histonet] Best Marker for Proliferation?
Histonetters,
Good day. Thanks to all of you that replied to my previous message on
CD31/PECAM-1 staining. I now have another question to pose.
What is the 'best' marker to use for immunohistochemical detection of
cell proliferation on tissue slides from 4% paraformaldehyde-fixed,
paraffin-embedded mouse xenograft tissue (human prostate epithelial
cells mixed with mouse prostate stromal cells)? (Or, what is the most
current, widely-accepted method)
The markers I know of are Ki67, PCNA, Cdc2 and Brdu-kits (non-tritiated
thymidine method, ex: Roche cat 1 299 964), although I'm open to any
other experienced suggestions.
Thanks for your time and advice.
Sincerely,
Isaiah Schauer
3rd year graduate student
Lab of David R. Rowley, Ph.D.
Baylor College of Medicine
Department of Molecular and Cellular Biology
One Baylor Plaza, room 326D
Houston, TX 77030
lab: 713-798-6221
fax: 713-790-1275
3rd year graduate student
Lab of David R. Rowley, Ph.D.
Baylor College of Medicine
Department of Molecular and Cellular Biology
One Baylor Plaza, room 326D
Houston, TX 77030
lab: 713-798-6221
fax: 713-790-1275
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