[Histonet] detach cells from a slide....again
Favara, Cynthia (NIH/NIAID)
cfavara <@t> niaid.nih.gov
Wed Apr 14 15:30:20 CDT 2004
Proteinase K at a high conc. should do it.
c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
-----Original Message-----
From: Dolors Fuster [mailto:d.fuster <@t> ub.edu]
Sent: Wednesday, April 14, 2004 10:50 AM
To: HISTONET
Subject: [Histonet] detach cells from a slide....again
In my previous mail I forgot to tell you the mechanical method (scrape them
off
with a sharp razor blade) is made....but too slow for at least 600
slides...difficult to recover all stuff...some of recovered adheres to the
eppendorf.... :o(
Could the mehod not include trypsin in its procedure :o) ??
Thanks again
Any idea to detach cells from an hematologic slide?
They are dryed...stained with May Grünwald Giemsa...dehidrated, cleared and
mounted with DPX.
The idea is to use them for a DNA extraction.
Thanks in advance
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----- Fi del missatge reenviat -----
Dolors Fuster
Tècnic Especialista en Anatomia Patològica i Citologia
Departament d'Anatomia i Embriologia Humana
Facultat de Medicina (HCP)
Universitat de Barcelona
ICQ 14146798
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