[Histonet] Submit
Ze Lu
lu_ze <@t> sbcglobal.net
Tue Apr 13 15:40:51 CDT 2004
Topic: microtome
Dear histonet friends,
We are seting up a histological lab. Currently we are looking for a woring
microtone for paraffine block tissue sectioning. If someone can help, please
contact wirh me. Thanks.
Ze Lu, Dr.
Optimum Therapeutics, LLC
Email: optimum-t <@t> columbus.rr.com
----- Original Message -----
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 13, 2004 12:01 PM
Subject: Histonet Digest, Vol 5, Issue 20
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> Today's Topics:
>
> 1. RE: histogel (S Ladd)
> 2. Re: Vibratome Considerations (Geoff McAuliffe)
> 3. Histology Manager Opening at Yale University (Ramona Tolliver)
> 4. RE: histogel (Andrea Grantham)
> 5. Zinc formalin (Lorraine Cornett)
> 6. RE: histogel (Connie McManus)
> 7. RE: histogel (S Ladd)
> 8. RE: histogel (S Ladd)
> 9. RE: histogel (Bartlett, Jeanine)
> 10. RE: histogel (Bartlett, Jeanine)
> 11. RE: histogel (Patsy Ruegg)
> 12. Rat Turbinates (Antonia Abeyta)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 13 Apr 2004 10:49:12 -0400
> From: "S Ladd" <sladd <@t> hsc.usf.edu>
> Subject: RE: [Histonet] histogel
> To: "m. van mkempen" <m.vankempen <@t> erasmusmc.nl>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <EDEILFIMBEHLDJPKFIPIMEFECAAA.sladd <@t> hsc.usf.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The instructions
> say something vague like "process as usual". Of course, I informed Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the instructions.
> Once you have fixed the histogel you can process it like you would any
other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen <@t> erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 13 Apr 2004 11:02:38 -0700
> From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] Vibratome Considerations
> To: Danielle Zalinski <nsdnz <@t> neuro.hfh.edu>
> Cc: HistoNet Listserve <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <407C2B3E.40602 <@t> umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=us-ascii
>
> I have done immunos on vibratome sections of rat brain. If you use a 12
> well tissue culture plate (with its lid) on a shaker table overnight at
> room temp. you should get good results. You will need to add some Triton
> or Tween to the primary antibody (and probably subsequent steps) or do
> something to 'permeabilize' the sections (freeze-thaw or alcohol) so you
> get good penetration of the antibodies. If you have access to a sliding
> microtome with a freezing stage you could obtain sections that way.
>
> Geoff
>
> Danielle Zalinski wrote:
>
> > Hello All,
> >Our lab would like to do immunohistochem work in rat brain on vibratome
> >sections instead of paraffin. We are able to get sections about
> >50-60um. Will incubation with primary antibody overnight be sufficient?
> > Is shaking the sections an option? I was hoping some other labs that
> >are working in vibratome sections may have some beginners advice.
> >Thanks,
> >Danielle Zalinski
> >
> >Neurosurgery Research
> >Henry Ford Health System
> >Detroit, MI
> >
> >
> >CONFIDENTIALITY NOTICE: This email contains information from the sender
that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise
protected from disclosure. This email is intended for use only by the person
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> >
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 13 Apr 2004 11:14:18 -0400
> From: Ramona Tolliver <ramona.tolliver <@t> yale.edu>
> Subject: [Histonet] Histology Manager Opening at Yale University
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <6.0.1.1.2.20040413111131.01e75d48 <@t> email.med.yale.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Good morning,
>
> Yale University has a Histology Manager (1st shift) position available
from
> 7:00 a.m. - 3:00 p.m. If you know of anyone who may be interested in an
> outstanding growth opportunity in a department committed to excellence in
> patient care, teaching, and discovery, please forward this information on
> to them.
>
> Salary is commensurate with experience. Relocation assistance is
> available. The position is posted online at :
> http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp-2&job=11326 .
>
> Inquiries should be sent to ramona.tolliver <@t> yale.edu. Thank you for your
time!
>
>
>
> Ramona E. Tolliver
>
> *If you have difficulty with the link provided above, please go to
> www.yale.edu/jobs and search Managerial and Professional last two weeks
and
> Pathology. The position is listed as Manager III.
>
>
>
>
> Ramona E. Tolliver
> Human Resource Manager
> Yale University School of Medicine
> Department of Pathology
> 310 Cedar Street
> New Haven, CT 06520-8023
> Telephone: (203) 785-6689
> Fax: (203) 785-7303
>
> ------------------------------
>
> Message: 4
> Date: Tue, 13 Apr 2004 08:28:11 -0700
> From: Andrea Grantham <algranth <@t> u.arizona.edu>
> Subject: RE: [Histonet] histogel
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <4.3.2.7.2.20040413082229.00cb9f10 <@t> algranth.inbox.email.arizona.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
concentration
> of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky?
> Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
instructions
> >say something vague like "process as usual". Of course, I informed
Richard
> >Allen that many of us in research do not have formalin on our tissue
> >processors. Richard Allen said they were going to change the
instructions.
> >Once you have fixed the histogel you can process it like you would any
other
> >piece of tissue.
> >Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding.
> >My question is do I have to fix the histogel before processing for
> >embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen <@t> erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 13 Apr 2004 11:32:55 -0400
> From: "Lorraine Cornett" <cornettl <@t> hotmail.com>
> Subject: [Histonet] Zinc formalin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY7-F11l7tMmXm4yp40004c0d3 <@t> hotmail.com>
> Content-Type: text/plain; format=flowed
>
> We are currentyl processing our biopsies in 10% neutral buffered formalin
> (short process cycle). Our pathologists miss the nuclear detail they
became
> accustomed to when we were processing in Zinc Formalin. We seem to
remember
> a procedure where you can "mordant" or post fix the actual slides before
> staining. Does anyone have this procedure that they can share with me.
>
> Thanks,
>
> Lorraine Cornett (HT ASCP)
> Blue Ridge Pathology
> Kingsport, TN 37660
> 423 224-5793
>
> _________________________________________________________________
> Is your PC infected? Get a FREE online computer virus scan from McAfee®
> Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 13 Apr 2004 09:24:43 -0600
> From: "Connie McManus" <convmcm <@t> cc.usu.edu>
> Subject: RE: [Histonet] histogel
> To: "'S Ladd'" <sladd <@t> hsc.usf.edu>, "'m. van mkempen'"
> <m.vankempen <@t> erasmusmc.nl>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <000101c4216b$72f8c5e0$4a737b81 <@t> Cygnus>
> Content-Type: text/plain; charset="us-ascii"
>
> Forgive my ignorance, but what is histogel and what is it used for?
> thanx
>
> Connie McManus
> Utah Veterinary Diagnostics Laboratory
> Utah State University
> Logan, UT
> Phone: 435/797-1891
> fax: 435/797-2805
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of S Ladd
> Sent: Tuesday, April 13, 2004 7:49 AM
> To: m. van mkempen
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The
> instructions
> say something vague like "process as usual". Of course, I informed
> Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the
> instructions.
> Once you have fixed the histogel you can process it like you would any
> other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen <@t> erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 13 Apr 2004 11:42:46 -0400
> From: "S Ladd" <sladd <@t> hsc.usf.edu>
> Subject: RE: [Histonet] histogel
> To: "Andrea Grantham" <algranth <@t> u.arizona.edu>
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <EDEILFIMBEHLDJPKFIPIEEFGCAAA.sladd <@t> hsc.usf.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> Are the items you are embedding in the histogel fixed in formalin?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Andrea
> Grantham
> Sent: Tuesday, April 13, 2004 11:28 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
concentration
> of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky?
> Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
instructions
> >say something vague like "process as usual". Of course, I informed
Richard
> >Allen that many of us in research do not have formalin on our tissue
> >processors. Richard Allen said they were going to change the
instructions.
> >Once you have fixed the histogel you can process it like you would any
> other
> >piece of tissue.
> >Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding.
> >My question is do I have to fix the histogel before processing for
> >embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen <@t> erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 13 Apr 2004 11:42:45 -0400
> From: "S Ladd" <sladd <@t> hsc.usf.edu>
> Subject: RE: [Histonet] histogel
> To: "Kyle-Byrne, Carrie - Labvision" <CKByrne <@t> labvision.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <EDEILFIMBEHLDJPKFIPICEFGCAAA.sladd <@t> hsc.usf.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Since your cells were fixed in formalin, perhaps there was enough residual
> formalin to sufficiently fix the histogel? You use only a few drops of
> histogel. My cells were not in formalin and I use about 0.5 mL of
histogel.
> Just for fun, you could try processing a little button of histogel
(without
> formalin fixed cells) and see if it dissolves?
> I use ethanol and xylene on my processor and I also start with 70%
ethanol.
> Sharron
>
> -----Original Message-----
> From: Kyle-Byrne, Carrie - Labvision [mailto:CKByrne <@t> labvision.com]
> Sent: Tuesday, April 13, 2004 11:23 AM
> To: 'S Ladd'
> Subject: RE: [Histonet] histogel
>
>
> Sharron,
> Interesting. I've never heard of this before. I've used Histogel for
years
> and never had to 'fix' it in formalin. I used it to make cell pellets for
> use as IHC control material. I would fix the cells in formalin first then
> transfer to 70% EtOH and hold them until I had several to pellet. I'd
> decant the 70%, put on a few drops of Histogel, mix to distribute the
cells,
> then cool the tube to harden the gel. Once solidified, I'd remove the
> pellet from the tube and breadloaf it like a piece of tissue, place in a
> cassette and onto the processor (we started in 70% EtOH) for overnight
> processing and embed as usual in the morning. It always worked just fine.
> What to you use for clearing?
>
> Carrie Kyle-Byrne
> Sr. Reseach Assoc., Pathology Lab
> Lab Vision, Corp.
> 47791 Westinghouse Ave.
> Fremont, CA 94539
> ckbyrne <@t> labvision.com
> www.labvision.com
>
>
>
> -----Original Message-----
> From: S Ladd [mailto:sladd <@t> hsc.usf.edu]
> Sent: Tuesday, April 13, 2004 7:49 AM
> To: m. van mkempen
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The instructions
> say something vague like "process as usual". Of course, I informed Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the instructions.
> Once you have fixed the histogel you can process it like you would any
other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen <@t> erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 13 Apr 2004 11:43:09 -0400
> From: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] histogel
> To: "Andrea Grantham" <algranth <@t> u.arizona.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CB857F6460D42E4AAEA195054A25406C02F5E4CD <@t> m-ncid-2.ncid.cdc.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> We do the same and have had no problems.
>
> Jeanine Bartlett, HT(ASCP)
> Centers for Disease Control
> Infectious Disease Pathology Activity
> 1600 Clifton Road, MS/G-32
> Atlanta, GA 30333
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
> Grantham
> Sent: Tuesday, April 13, 2004 11:28 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
> concentration
> of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky?
> Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
> >instructions say something vague like "process as usual". Of course, I
> >informed Richard Allen that many of us in research do not have formalin
>
> >on our tissue processors. Richard Allen said they were going to change
> >the instructions. Once you have fixed the histogel you can process it
> >like you would any other piece of tissue. Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding. My question is do I have to fix the
> >histogel before processing for embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen <@t> erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 13 Apr 2004 11:47:04 -0400
> From: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] histogel
> To: "S Ladd" <sladd <@t> hsc.usf.edu>, "Andrea Grantham"
> <algranth <@t> u.arizona.edu>
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CB857F6460D42E4AAEA195054A25406C02F5E4CF <@t> m-ncid-2.ncid.cdc.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> Yes.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of S Ladd
> Sent: Tuesday, April 13, 2004 11:43 AM
> To: Andrea Grantham
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> Are the items you are embedding in the histogel fixed in formalin?
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Andrea
> Grantham
> Sent: Tuesday, April 13, 2004 11:28 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
> concentration of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky? Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
> >instructions say something vague like "process as usual". Of course, I
> >informed Richard Allen that many of us in research do not have formalin
>
> >on our tissue processors. Richard Allen said they were going to change
> >the instructions. Once you have fixed the histogel you can process it
> >like you would any
> other
> >piece of tissue.
> >Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding. My question is do I have to fix the
> >histogel before processing for embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen <@t> erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 13 Apr 2004 10:00:05 -0600
> From: "Patsy Ruegg" <pruegg <@t> colobio.com>
> Subject: RE: [Histonet] histogel
> To: "S Ladd" <sladd <@t> hsc.usf.edu>, "m. van mkempen"
> <m.vankempen <@t> erasmusmc.nl>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <NGBBIMHHKLGNCOCPDKBOEEHBCGAA.pruegg <@t> colobio.com>
> Content-Type: text/plain; charset="us-ascii"
>
> I use histogel all the time without fixing in formalin. Like Andi I start
> with tissue already fixed and then placed in 70% alcohol. I embed this
> tissue in histogel and put it back in the 70% and then process starting
with
> 70% alcohol, no formalin. I have not had the histogel ever dissolve.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of S Ladd
> Sent: Tuesday, April 13, 2004 8:49 AM
> To: m. van mkempen
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The instructions
> say something vague like "process as usual". Of course, I informed Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the instructions.
> Once you have fixed the histogel you can process it like you would any
other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen <@t> erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 13 Apr 2004 10:57:18 -0600
> From: "Antonia Abeyta" <ALAbeyta <@t> salud.unm.edu>
> Subject: [Histonet] Rat Turbinates
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s07bc799.041 <@t> salud.unm.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi all,
>
> I am planning on staining some rat turbinates with Alcian Blue, and
> counter-staining with Hematoxylin & Eosin. Some of the literature
> suggests also adding a Periodic Acid Schiff (PAS) step into the
> protocol. What exactly does this step change and what is the PAS step
> useful for?
>
> Also, if anyone has some pointers for keeping the rat turbs from
> floating off the slides...we are currently using charged slides but they
> don't seem to be working.
>
> Thanks.
>
> Antonia L. Abeyta
> Health Sciences Tech. III
> Community Environmental Health Program
> University of New Mexico
> Surge Bldg. Room 140
> Albuquerque, NM 87131
> (505) 272-4028
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 5, Issue 20
> ***************************************
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