[Histonet] Rat Turbinates

[Antonia Abeyta]

Hi all,

I am planning on staining some rat turbinates with Alcian Blue, and
counter-staining with Hematoxylin & Eosin.  Some of the literature
suggests also adding a Periodic Acid Schiff (PAS) step into the
protocol.  What exactly does this step change and what is the PAS step
useful for?

Also, if anyone has some pointers for keeping the rat turbs from
floating off the slides...we are currently using charged slides but they
don't seem to be working.

Thanks.

Antonia L. Abeyta
Health Sciences Tech. III
Community Environmental Health Program
University of New Mexico
Surge Bldg. Room 140
Albuquerque, NM  87131
(505) 272-4028