[Histonet] histogel
Bartlett, Jeanine
jqb7 <@t> cdc.gov
Tue Apr 13 10:43:09 CDT 2004
We do the same and have had no problems.
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Grantham
Sent: Tuesday, April 13, 2004 11:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] histogel
When I use histogel I do "process as usual" and this does not include
formalin. I put the cassette containing the tissue embedded in histogel
into 70% ETOH. The processor starts with 70% ETOH X 2 and the
concentration
of ETOH goes up from there. Haven't had any problems.
Maybe I've just been lucky?
Andi Grantham
At 10:49 AM 4/13/2004 -0400, you wrote:
>Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
>processing it WILL DISSOLVE! I learned this the hard way! The
>instructions say something vague like "process as usual". Of course, I
>informed Richard Allen that many of us in research do not have formalin
>on our tissue processors. Richard Allen said they were going to change
>the instructions. Once you have fixed the histogel you can process it
>like you would any other piece of tissue. Sharron
>University of South Florida
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
>mkempen
>Sent: Tuesday, April 13, 2004 10:18 AM
>To: Histonetlist
>Subject: [Histonet] histogel
>
>
>Dear all,
>
>I have a question concerning histogel!!
>I'm working with fetal lung and I thought histogel would be a nice way
>to handle then while embedding. My question is do I have to fix the
>histogel before processing for embedding in paraffine?
>How is the normal procedure for tissues's in histogel??
>
>Thank you for all your kind answers.
>
>Regards,
>Marta.
>
>--
>----------------------------
>*- mkempen -*
>MAILTO:m.vankempen <@t> erasmusmc.nl
>-----------------------------------------
>
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.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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