[Histonet] histogel

[S Ladd]

Are the items you are embedding in the histogel fixed in formalin?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Andrea
Grantham
Sent: Tuesday, April 13, 2004 11:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] histogel


When I use histogel I do "process as usual" and this does not include
formalin. I put the cassette containing the tissue embedded in histogel
into 70% ETOH. The processor starts with 70% ETOH X 2 and the concentration
of ETOH goes up from there. Haven't had any problems.
Maybe I've just been lucky?
Andi Grantham



At 10:49 AM 4/13/2004 -0400, you wrote:
>Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
>processing it WILL DISSOLVE! I learned this the hard way! The instructions
>say something vague like "process as usual". Of course, I informed Richard
>Allen that many of us in research do not have formalin on our tissue
>processors. Richard Allen said they were going to change the instructions.
>Once you have fixed the histogel you can process it like you would any
other
>piece of tissue.
>Sharron
>University of South Florida
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
>mkempen
>Sent: Tuesday, April 13, 2004 10:18 AM
>To: Histonetlist
>Subject: [Histonet] histogel
>
>
>Dear all,
>
>I have a question concerning histogel!!
>I'm working with fetal lung and I thought histogel would be a nice way
>to handle then while embedding.
>My question is do I have to fix the histogel before processing for
>embedding in paraffine?
>How is the normal procedure for tissues's in histogel??
>
>Thank you for all your kind answers.
>
>Regards,
>Marta.
>
>--
>----------------------------
>*- mkempen -*
>MAILTO:m.vankempen <@t> erasmusmc.nl
>-----------------------------------------
>
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.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


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