[Histonet] histogel

[Connie McManus]

Forgive my ignorance, but what is histogel and what is it used for?
thanx

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of S Ladd
Sent: Tuesday, April 13, 2004 7:49 AM
To: m. van mkempen
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] histogel

Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
processing it WILL DISSOLVE! I learned this the hard way! The
instructions
say something vague like "process as usual". Of course, I informed
Richard
Allen that many of us in research do not have formalin on our tissue
processors. Richard Allen said they were going to change the
instructions.
Once you have fixed the histogel you can process it like you would any
other
piece of tissue.
Sharron
University of South Florida

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of m. van
mkempen
Sent: Tuesday, April 13, 2004 10:18 AM
To: Histonetlist
Subject: [Histonet] histogel


Dear all,

I have a question concerning histogel!!
I'm working with fetal lung and I thought histogel would be a nice way
to handle then while embedding.
My question is do I have to fix the histogel before processing for
embedding in paraffine?
How is the normal procedure for tissues's in histogel??

Thank you for all your kind answers.

Regards,
Marta.

--
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*- mkempen -*
MAILTO:m.vankempen <@t> erasmusmc.nl
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