[Histonet] VEGF

[Kyle-Byrne, Carrie - Labvision]

Luis,
I would think the quickest way to have a good idea is to check the aa
alignments of human v mouse.  the Santa Cruz mono antibody is raised to aa
1-140 of human VEGF.  I'd suggest having a look at the mouse alignment and
see what the homology is.  According to Santa Cruz, it should cross react,
but my own experience with Santa Cruz antibodies in general has mirrored
that of many others....you just won't really know until you try it.


Carrie Kyle-Byrne
Lab Vision, Corp.
www.labvision.com



-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
Sent: Monday, April 12, 2004 8:49 AM
To: Luis Chiriboga; Mass Histology Service; Amos Brooks;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


Luis,
I have not tried the mono vegf on mice but I have used it successfully on
rat tissue.  In my hands the poly vegf is so non-specific I could not ever
get it cleaned up enough to be worth anything.
Patsy

-----Original Message-----
From: Luis Chiriboga [mailto:Luis.Chiriboga <@t> med.nyu.edu]
Sent: Monday, April 12, 2004 9:39 AM
To: Patsy Ruegg; Mass Histology Service; Amos Brooks;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


Have any of you tried it on mouse?????? I have stuck with the polyclonal (on
mouse tissue) to avoid the species issue but it's so dirty anyway.  I'm
figuring I can at least try and manipulate the species issue (high dilution,
absorbed secondary, blocking, biotinylating primary etc...) and get better
results. Would you recommend?

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