[Histonet] BMP-4 antibody staining in murine tissue

[Johnson, Teri]

Has anybody had any success in immunostaining BMP-4 in mouse tissue?  We
are using EDTA decalcified, formalin fixed and paraffin embedded bone
specimens, and are using DAKO's ARK kit for the mouse on mouse
techniques.  We have tried using trypsin digestion, epitope unmasking
with citrate buffer, and no pretreatment. There are many other
pretreatments to use, but before we unleash the arsenal, I thought I'd
ask.

Thanks for any assistance,

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org