[Histonet] VEGF
Sebree Linda A.
la.sebree <@t> hosp.wisc.edu
Sat Apr 10 16:09:09 CDT 2004
Jim,
Did you find there was a problem with oxidation? A researcher has brought us a number of renal biopsy slides for VEGF staining, some which have been cut for a couple years and I presume stored at R. T., and I'm afraid we may have an issue with antigenicity of the epitope. What do you think?
Linda Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
-----Original Message-----
From: Mass Histology Service [mailto:jstaruk <@t> masshistology.com]
Sent: Fri 4/9/2004 3:57 PM
To: Patsy Ruegg; Amos Brooks; histonet <@t> lists.utsouthwestern.edu
Cc:
Subject: RE: [Histonet] VEGF
We just completed a large study on human prostate biopsies using the Santa
Cruz monoclonal VEGF (C1) following pronase pre-treatment and they all came
out wonderful: Very clean and the negative controls (no primary) were
completely negative.
Jim
____________________
James Staruk, HT(ASCP)
Mass Histology Service
www.masshistology.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Patsy
Ruegg
Sent: Friday, April 09, 2004 3:38 PM
To: Amos Brooks; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF
Amos,
I totally agree with you about Santa Cruz's poly VEGF but the mono was much
better. I have a customer who still wants to use the poly vegf on sheep
placenta and I think it is awful but you have to give the customer what they
want. I did a comparison with the poly and mono for them but they still
opted for the poly because it matched their molecular studies. Oh well. To
me it is all over the place but they seem to think that is expected for
their samples, they think is is real and call it "an expression antibody" or
something like that.
Patsy
(snip)
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