[Histonet] Re: Histonet Digest, Vol 5, Issue 11

[Patti Loykasek]

Amos & all - I couldn't have said it better myself. The only difference is
that we have tried >6 clones. Be very careful of the staining, read up on
the expected positivity, and pick your positive controls carefully.

Patti Loykasek


> Linda,
>        Sorry to say there's no such thing. VEGF, despite any
> specifications, just doesn't work properly. We've tried at least 4
> different vendors with hardly any success. Either it didn't label at all,
> labeled in all the wrong places, or the negative control looked the same as
> the test tissue. Check the controls closely if you try this. It's just not
> a reliable antibody.
> Regards,
> Amos Brooks
> 
> At 09:20 AM 4/8/2004, you wrote:
>> Message: 2
>> Date: Wed, 7 Apr 2004 13:00:57 -0500
>> From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
>> Subject: [Histonet] VEGF antibody
>> To: "Histonet (E-mail)" <histonet <@t> pathology.swmed.edu>
>> Message-ID:
>>         <D6B654003615874B873E15BA680E2D22F80AEB <@t> uwhis-xchng1.hosp.wisc.edu>
>> Content-Type: text/plain;       charset="iso-8859-1"
>> 
>> Hi again,
>> 
>> Wondering if anyone can recommend a good, reliable VEGF (vascular
>> endothelial growth factor) antibody?  If you've run yours on a Ventana
>> instrument, so much the better.
>> 
>> Thanks for the help,
>> 
>> Linda A. Sebree
>> University of Wisconsin Hospital & Clinics
>> IHC/ISH Clinical & Research Laboratory
>> DM223-VA
>> 600 Highland Ave.
>> Madison, WI 53792
>> (608)265-6596
>> FAX:  (608)262-7174
> 
> 
> 
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