[Histonet] Re: Histonet Digest, Vol 5, Issue 11

[Patsy Ruegg]

Amos I had the same experience as you with VEGF's until I switched to
monoclonal Vegf from Santa Cruz, it now appears very specific and reliable
to me.  I use pepsin or proteinase K digestion and stay away from HIER.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Amos
Brooks
Sent: Thursday, April 08, 2004 4:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 5, Issue 11


Linda,
         Sorry to say there's no such thing. VEGF, despite any
specifications, just doesn't work properly. We've tried at least 4
different vendors with hardly any success. Either it didn't label at all,
labeled in all the wrong places, or the negative control looked the same as
the test tissue. Check the controls closely if you try this. It's just not
a reliable antibody.
Regards,
Amos Brooks

At 09:20 AM 4/8/2004, you wrote:
>Message: 2
>Date: Wed, 7 Apr 2004 13:00:57 -0500
>From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
>Subject: [Histonet] VEGF antibody
>To: "Histonet (E-mail)" <histonet <@t> pathology.swmed.edu>
>Message-ID:
>
<D6B654003615874B873E15BA680E2D22F80AEB <@t> uwhis-xchng1.hosp.wisc.edu>
>Content-Type: text/plain;       charset="iso-8859-1"
>
>Hi again,
>
>Wondering if anyone can recommend a good, reliable VEGF (vascular
>endothelial growth factor) antibody?  If you've run yours on a Ventana
>instrument, so much the better.
>
>Thanks for the help,
>
>Linda A. Sebree
>University of Wisconsin Hospital & Clinics
>IHC/ISH Clinical & Research Laboratory
>DM223-VA
>600 Highland Ave.
>Madison, WI 53792
>(608)265-6596
>FAX:  (608)262-7174



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