[Histonet] BK and JC by IHC

[Stapf, Ross]

We do BK and just started to do JC.  We get JC from Oncogene(sp)
1-858-450-5558, cat# DP02 200ug.

We just got it to work using Dako HighPH TR, 1:100 overnight.  Then used
a Ventana Detection kit to finish it off on the machine after the
overnight incubation.  The slides are still not perfect yet so you will
definitely have to tweek this, but it gives you someplace to start.

We have been doing BK for a little while.  Let me know if you have any
questions about that one and I'll find out.  Our immuno techs here do a
great job.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS <@t> baylorhealth.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lynn
McKee
Sent: Tuesday, April 06, 2004 11:56 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] BK and JC by IHC


Hi everyone,

    Is anyone doing IHC for BK and JC virus? We do INSITU but our Dr.
wants to do IHC now. I found CHEMICON carries the BK but I have had no
luck with JC. Thanks in advance for your help.

Lynn McKee
University of Cininnati


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