[Histonet] baking bone slides for heat retrieval techniques?

[Garlits, John]

I have tried repeatedly to use boiling or heated antigen retrieval techniques on my bone slides, but the bone always peels off.  (The marrow stays.)

I did hear somewhere that in this case one can heat the slides at around 55 degrees C overnight, which makes the bone adhere better to potentially survive boiling antigen retrieval.  Has anyone tried this or know if this works?

Thanks!

John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
Phone: 901-495-3961
Fax: 901-495-2176