[Histonet] HELP!!!

[Jorge Ivan Zapata Valencia]

Hello everybody.
Cordial greetings.
I have been trying immunostaining for CD markers on mouse tissue samples. I 
have gotten some really nice slides but............sometimes the edges of 
the samples turn brown but the center continues completely clear. Even the 
negative control sometimes is completely clear and the other samples brown 
edges. I do not know if my problem is that I am allowing the samples to dry 
up, I am not completely covering samples with the antibody solutions or 
............????. May one help me to find what is the problem and how to 
solve it??
Thank You very much.
Regards,


JORGE IVAN ZAPATA
Universidad del Valle

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